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2 results on '"Shannon Ginty"'

1. Protocol and statistical analysis plan for the PREventing cardiovascular collaPse with Administration of fluid REsuscitation during Induction and Intubation (PREPARE II) randomised clinical trial

Author

Shekhar Ghamande, Bruno Pereira, Christopher J Lindsell, Victor E Ortega, Aaron M Joffe, Heath D White, Muhammad Ali, David R Janz, Kevin M Dischert, Emily Adams, James M Dargin, A M Joffe, Akram Khan, Simanta Dutta, Joanne M Wozniak, Susan Stempek, Olivia F Krol, Brian E Driver, Joseph M Brewer, Stephen P Peters, Rita N Bakhru, Scott Bauer, Christina R Bellinger, Amanda M Brown, Blair Brown, Jerri Brown, Caitlin Bumgarner, Wendy Butcher, Megan Caudle, Arjun B Chatterjee, David J Chodos, Gerardo Corcino, Nathan S Cutler, Travis L Dotson, Daniel C Files, Jonathan L Forbes, Katherine A Gershner, Shannon Ginty, Kiadrick R Hood, April Hazelwood, Katherine Hendricks, Kelly Jacobus, Jonathan T Jaffe, Stacy Kay, Chad A Kloefkorn, Jennifer Krall, Margo T Lannan, Cornelia Lane, Cynthia Lanning, Jessica Lyons, William I Mariencheck, Chad R Marion, Matthew A Maslonka, Sara McClintock, Nathaniel M Meier, Matthew C Miles, Peter J Miller, Sophia Mitchell, Wendy C Moore, Katherine Moss, Andrew M Namen, Dustin L Norton, Stella B Ogake, Jill A Ohar, Jessica A Palakshappa, Rodolfo M Pascual, Sandi Pascual, Aaron Pickens, Adam R Schertz, Matt Strong, Alexander O Sy, Braghadheeswar Thyagarajan, Amy Townsend, Russell Worthen, Michael Wlodarski, Charles Yarbrough, Caroline York, James Dargin, Joanne Wozniak, Christopher Adler, Ahmed Agameya, Michael Colancecco, Daniel Fitelson, Joshua Giaccotto, Gena Han, Louise Kane, Ezra Miller, Timothy Noland, Jaqueline Price, Joseph Plourde, Fraser Mackay, Laura Mahoney, Avignat Patel, Michael Plourde, Zena Saadeh, Sara Shadchehr, Sandeep Somalaraju, Eleanor Summerhill, Ryan Webster, Jordan Winnicki, Ekaterina Yavarovich, D Sheylan, Alejandro C Arroliga, Tasnim Lat, Stephanie Nonas, Milad K Jouzestani, Raya Adi, Chandani Anandkat, Hanae Benchbani, Matthew G Drake, Makrina N Kamel, Ramanpreet Randhawa, and Jessica L Tsui

Subjects
Medicine
Published
2020
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2. DNA Fingerprint Analysis of Three Short Tandem Repeat (STR) Loci for Biochemistry and Forensic Science Laboratory Courses.

Author

Kathleen McNamara-Schroeder, Cheryl Olonan, Simon Chu, Maria C. Montoya, Mahta Alviri, Shannon Ginty, and John J. Love

Subjects
DNA fingerprinting, FORENSIC genetics techniques, FORENSIC sciences, CHEMICAL laboratories, BIOCHEMISTRY, CURRICULUM laws & legislation, CRIMINAL justice system, NATIONAL security
Abstract

We have devised and implemented a DNA fingerprinting module for an upper division undergraduate laboratory based on the amplification and analysis of three of the 13 short tandem repeat loci that are required by the Federal Bureau of Investigation Combined DNA Index System (FBI CODIS) data base. Students first collect human epithelial (cheek) cells using sterile buccal swabs and then utilize commercially available kits and materials to extract genomic DNA. This is followed by the PCR amplification of three specific short tandem repeat loci (i.e. CSFIPO, TPOX, THO1). Polyacrylamide gel electrophoresis is used to resolve the allelic bands associated with the three short tandem repeat loci, and the results are statistically analyzed in the context of human population genetics. In addition, DNA was collected from a family, and the children's allele sets were compared with those of the parents to help illustrate paternal and maternal relatedness. This module enables students to use the materials and methods employed by actual law enforcement agencies and therefore can be used for laboratory exercises in traditional biochemistry curricula as well as for the growing field of forensic science and education. [ABSTRACT FROM AUTHOR]

Published
2006
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