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This study investigated the biofilm-forming capabilities of Enterococcus isolates from Portuguese traditional cheeses with protected designation of origin (PDO) status, specifically Azeitão and Nisa. Given the absence of added starter cultures in the cheesemaking process, the characteristics of these cheeses are intrinsically linked to the autochthonous microbiota present in the raw materials and the production environment. Our findings demonstrate that all isolates possess biofilm production abilities, which are crucial for their colonization and persistence within cheese factories, thereby maintaining factory-specific microbial heritage. Through an integrated analysis utilizing principal component analysis (PCA), a direct correlation between biofilm formation and cell viability was established. Notably, these results underscore the adaptive capacity of enterococci to survive environmental fluctuations and their role in the unique characteristics of Portuguese traditional cheeses. Overall, this research enhances our understanding of the microbial dynamics in cheese production and highlights the importance of enterococci in preserving cheese quality and heritage. [ABSTRACT FROM AUTHOR]

This letter to the editor published in the Annals of Laboratory Medicine discusses the measurement of low-density lipoprotein cholesterol (LDL-C) levels and compares different methods for accuracy and speed. The study found that the direct LDL-C assay and Martin-Hopkins equation were the most accurate alternatives to the reference method, particularly in patients with high triglyceride levels. However, for individuals with low LDL-C levels or high triglyceride levels, other equations tended to underestimate LDL-C levels. The study concludes that the direct LDL-C assay is the best choice for individuals with specific lipid profiles. [Extracted from the article]

The spread of pathogenic and food spoilage microorganisms through the food chain still faces major mitigation challenges, despite modern advances. Although multiple cleaning and disinfection procedures are available for microbial load reduction in food-related settings, microbes can still remain on surfaces, equipment, or machinery, especially if they have the ability to form biofilms. The present study assessed the biofilm-forming properties of pure and mixed cultures of foodborne and spoilage bacteria (Listeria monocytogenes, Enterococcus faecalis, Aeromonas hydrophila, Brochothrix thermosphacta), using polystyrene and stainless steel contact surfaces. Subsequently, the antimicrobial and antibiofilm properties of Thymus vulgaris and Cymbopogon flexuosus essential oils—EOs—were evaluated against these bacteria. Moreover, in silico prediction of the absorption and toxicity values of the EOs' major constituents was also performed, perceiving the putative application in food-related settings. Overall, biofilm formation was observed for all microbes under study, at different temperatures and both contact surfaces. In polystyrene, at 25 °C, when comparing pure with mixed cultures, the combination Listeria–Aeromonas achieved the highest biofilm biomass. Moreover, at 4 °C, increased biofilm formation was detected in stainless steel. Regarding thyme, this EO showed promising antimicrobial features (especially against A. hydrophila, with a MIC of 0.60 µg/µL) and antibiofilm abilities (MBEC of 110.79 µg/µL against L. monocytogenes, a major concern in food settings). As for lemongrass EO, the highest antimicrobial activity, with a MIC of 0.49 µg/µL, was also observed against L. monocytogenes. Overall, despite promising results, the in situ effectiveness of these essential oils, alone or in combination with other antimicrobial compounds, should be further explored. [ABSTRACT FROM AUTHOR]

Copyright of Canadian Veterinary Journal / Revue Vétérinaire Canadienne is the property of Canadian Veterinary Medical Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de la Asociación Odontológica Argentina is the property of Asociacion Odontologica Argentina and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Archivos Venezolanos de Farmacología y Terapéutica is the property of Archivos Venezolanos de Farmacologia y Terapeutica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Archivos Venezolanos de Farmacología y Terapéutica is the property of Archivos Venezolanos de Farmacologia y Terapeutica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Rammed earth is considered a very sustainable construction system due to its low embodied energy, long service life and high recyclability. However, authors found that there is a lack of experimental results at real scale regarding rammed earth thermal behaviour. For this reason, this paper is first focused on the characterization of two different types of earth in order to check the suitability of being used in rammed earth walls. After the characterization, two experimental cubicle-shape buildings were built in Barcelona and Puigverd de Lleida (Spain) in order to test the thermal behaviour of their walls in two different climatic conditions. Temperature profiles inside walls have been monitored using thermocouples and temperature profile of southern walls was analysed in free floating conditions during summer and winter periods of 2013. Results show that thermal amplitude from outside to inside temperatures are decreased by rammed earth walls, achieving constant temperatures in inner surface of southern walls. [ABSTRACT FROM AUTHOR]

Microorganisms colonize surfaces and develop biofilms through interactions that are not yet thoroughly understood, with important implications for water and wastewater systems. This study investigated the interactions between N-acyl homoserine lactone (AHL)-producing bacteria, yeasts and protists, and their contribution to biofilm development. Sixty-one bacterial strains were isolated from activated sludge and screened for AHL production, with Aeromonas sp. found to be the dominant AHL producer. Shewanella xiamenensis, Aeromonas allosaccharophila, Acinetobacter junii and Pseudomonas aeruginosa recorded the highest adherence capabilities, with S. xiamenensis being the most effective in surface colonization. Additionally, highly significant interactions (i.e. synergic or antagonistic) were described for dual and multistrain mixtures of bacterial strains (P. aeruginosa, S. xiamenensis, A. junii and Pseudomonas stutzeri), as well as for strongly adherent bacteria co-cultured with yeasts. In this last case, the adhered biomass in co-cultures was lower than the monospecific biofilms of bacteria and yeast, with biofilm observations by microscopy suggesting that bacteria had an antagonist effect on the whole or part of the yeast population. Finally, protist predation by Euplotes sp. and Paramecium sp. on Aeromonas hydrophila biofilms not only failed to reduce biofilm formation, but also recorded unexpected results leading to the development of aggregates of high density and complexity. [ABSTRACT FROM AUTHOR]

Buildings should be understood as a process that consumes energy in all their phases (design, construction, use and end-of-life) and, more specifically, the building envelope is clearly involved in all of them. For this reason, the International Energy Agency defines in its latest publication the improvement of building envelopes as one of the key points to reduce the energy consumption in buildings. In the present study, two sustainable construction systems based on rammed earth walls are adapted to modern requirements to be thermally tested and compared against three Mediterranean conventional systems under summer conditions. The experimentation was done by performing several experiments in free floating and controlled temperature conditions at real scale in five cubicle-shape buildings with inner dimensions 2.4 × 2.4 × 2.4 m. The purpose of this study is to demonstrate that more sustainable construction systems can be used instead of conventional ones, with higher embodied energy, and achieve similar thermal response. Results show that the reduction of rammed earth wall thickness strongly penalizes its thermal behavior. However, similar thermal response than conventional systems is reached when 6 cm of wooden insulation panels are added in the outer face of the cubicle-shape building. [ABSTRACT FROM AUTHOR]

Reactive amendments such as Portland and super-sulfate cements offer a promising technology for immobilizing metalloid contaminants such as mercury (Hg) in soils and sediments through sequestration in less bioavailable solid forms. Tidal marsh sediments were reacted with dissolved Hg(II) in synthetic seawater and fresh water solutions, treated with Portland cement and FeSO 4 amendment, and aged for up to 90 days. Reacted solids were analyzed with bulk sequential extraction methods and characterized by powder X-ray diffraction (XRD), electron microscopy, and synchrotron X-ray absorption spectroscopy at the Hg L III - and S K-edge. In amended sediments, XRD, SEM and sulfur K-edge XANES indicated formation of gypsum in seawater experiments or ettringite-type (Ca 6 Al 2 (SO 4 ) 3 (OH) 12 . 26H 2 O) phases in fresh water experiments, depending on the final solution pH (seawater ∼8.5; freshwater ∼10.5). Analysis of Hg EXAFS spectra showed Cl and Hg ligands in the first- and second-coordination shells at distances characteristic of a polynuclear chloromercury(II) salt, perhaps as a nanoparticulate phase, in both seawater and fresh water experiments. In addition to the chloromercury species, a smaller fraction (∼20–25%) of Hg was bonded to O atoms in fresh water sample spectra, suggesting the presence of a minor sorbed Hg fraction. In the absence of amendment treatment, Hg sorption and resistance to extraction can be accounted for by relatively strong binding by reduced S species present in the marsh sediment detected by S XANES. Thermodynamic calculations predict stable aqueous Hg–Cl species at seawater final pH, but higher final pH in fresh water favors aqueous Hg-hydroxide species. The difference in Hg coordination between aqueous and solid phases suggests that the initial Hg–Cl coordination was stabilized in the cement hydration products and did not re-equilibrate with the bulk solution with aging. Collectively, results suggest physical encapsulation of Hg as a polynuclear chloromercury(II) salt as the primary immobilization mechanism. [ABSTRACT FROM AUTHOR]

Six by-products are used as additives in adobe bricks to study the variability of mechanical properties by a three level design of experiments and to evaluate optima formulations. Corn plant, fescue, straw, and grounded olive stones are agricultural by-products; and rubber crumbs and polyurethane are wastes used as transport and appliances by-products. Results show that the three-level design of experiments defines properly the governing equations of flexural and compression strength, furthermore it shows the interaction between them. A maximum improvement of 89% and 26% of flexural strength in corn plant and fescue types, respectively, is achieved. [ABSTRACT FROM AUTHOR]

Gypsum has two important states (fresh and hardened states), and the addition of phase change materials (PCM) can vary the properties of the material. Many authors have extensively studied properties in the hardened state; however, the variation of fresh state properties due to the addition of Micronalr® DS 5001 X PCM into gypsum has been the object of few investigations. Properties in fresh state define the workability, setting time, adherence and shrinkage, and, therefore the possibility of implementing the material in building walls. The aim of the study is to analyze, compare and evaluate the variability of fresh state properties after the inclusion of 10% PCM. PCM are added into a common gypsum matrix by three different methods: adding microencapsulated PCM, making a suspension of PCM/water, and incorporating PCM through a vacuum impregnation method. Results demonstrate that the inclusion of PCM change completely the water required by the gypsum to achieve good workability, especially the formulation containing Micronalr DS 5001 X: the water required is higher, the retraction is lower (50% less) due to the organic nature of the PCM with high elasticity and, the adherence is reduced (up to 45%) due to the difference between the porosity of the different surfaces as well as the surface tension difference. [ABSTRACT FROM AUTHOR]

The yeast community was studied in a municipal full-scale membrane bioreactor wastewater treatment plant (MBR-WWTP). The unexpectedly high diversity of yeasts indicated that the activated sludge formed a suitable environment for them to proliferate, with cellular concentrations of 2.2 ± 0.8 × 103 CFU ml−1. Sixteen species of seven genera were present in the biological reactor, with Ascomycetes being the most prevalent group (93%). Most isolates were able to grow in a synthetic wastewater medium, adhere to polyethylene surfaces, and develop biofilms of variable complexity. The relationship between yeast populations and the protists in the MBR-WWTP was also studied, revealing that some protist species preyed on and ingested yeasts. These results suggest that yeast populations may play a role in the food web of a WWTP and, to some extent, contribute to membrane biofouling in MBR systems. [ABSTRACT FROM AUTHOR]

Highlights: [•] Three types of stabilized rammed earth doped with microencapsulated PCM are studied. [•] The stabilizers used were straw, pneumatic fibres, lime, and alabaster. [•] DoE was used to optimize the material evaluating compressive strength. [•] Thermal properties of the optimum formulations were analyzed by a homemade device. [•] Optimum environmental impact was evaluated by LCA during the manufacturing phase. [Copyright &y& Elsevier]

Uptake and molecular speciation of dissolved Hg during formation of Al- or Fe-ettringite-type and high-pH phases were investigated in coprecipitation and sorption experiments of sulfate-cement treatments used for soil and sediment remediation. Ettringite and minor gypsum were identified by XRD as primary phases in Al systems, whereas gypsum and ferrihydrite were the main products in Hg-Fe precipitates. Characterization of Hg-Al solids by bulk Hg EXAFS, electron microprobe, and microfocused-XRF mapping indicated coordination of Hg by Cl ligands, multiple Hg and Cl backscattering atoms, and concentration of Hg as small particles. Thermodynamic predictions agreed with experimental observations for bulk phases, but Hg speciation indicated lack of equilibration with the final solution. Results suggest physical encapsulation of Hg as a polynuclear chloromercury(II) salt in ettringite as the primary immobilization mechanism. In Hg-Fe solids, structural characterization indicated Hg coordination by O atoms only and Fe backscattering atoms that is consistent with inner-sphere complexation of Hg(OH)20 coprecipitated with ferrihydrite. Precipitation of ferrihydrite removed Hg from solution, but the resulting solid was sufficiently hydrated to allow equilibration of sorbed Hg species with the aqueous solution. Electron microprobe XRF characterization of sorption samples with low Hg concentration reacted with cement and FeSO4 amendment indicated correlation of Hg and Fe, supporting the interpretation of Hg removal by precipitation of an Fe(III) oxide phase. [ABSTRACT FROM AUTHOR]