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We announce the sequence of the Escherichia coli MTR_GS_S1457 strain isolated from a soil sample of a vegetable gardening system for the first time in Bangladesh. With a length of 4,918,647 bp, this strain contained one plasmid, two CRISPR arrays, 54 predicted antibiotic resistance genes, and 81 predicted virulence factor genes.

This study announces the genome sequence of the Shigella flexneri MTR_GR_V146 strain isolated from a tomato (Solanum lycopersicum) sample in Bangladesh. This strain has a 4,624,521 bp genome length (coverage: 73.07×), 2 CRISPR arrays, 1 plasmid, 52 predicted antibiotic resistance genes, and 53 virulence factor genes.

We announce a genome sequence of Citrobacter freundii MTR_GS_V1777 strain isolated from a vegetable sample in Bangladesh. This strain had a genome size of 4,997,753 bp (58.7× genome coverage) and contained two plasmids, typed as sequence type ST124, 38 predicted antibiotic resistance genes, and 77 predicted virulence factor genes.

Reports indicate that vegetables are becoming a source of multidrug-resistant (MDR) bacteria, including Escherichia coli. Here, we present genome sequences of five MDR E. coli strains to assist future genomic analysis of this bacterium. These E. coli strains were isolated from vegetable samples of different gardening systems in Dhaka, Bangladesh.

Sustainable food systems require mechanisms that assure consumers about the sustainability of agricultural production. Building on the existing literature on the impact of sustainability standards, this study is the first to assess the effectiveness of participatory guarantee systems (PGS) for the certification of organic produce. The study uses representative farm-level data on local vegetable value chains in northern Vietnam and uses a broad set of sustainability outcomes as well as counterfactual analysis, including systematic robustness checks. The results show that PGS significantly improves farm profitability (+117%), agroecology performance (+40%), and gives farmers more choice of sales channels (+23%). However, PGS had no significant effect on returns to labour and reduced the average crop yield. Capacity development on nursery practices, transplanting of healthy seedlings rather than direct seeding, reduced tillage, and collective crop planning and management are some of the innovations that can counter adverse effects on crop yields, increase soil health as well as improve returns to labour, and thus attract more youth to farming. Overall, the study shows that organic PGS can make vegetable production more economically viable and more agroecologically sustainable. [ABSTRACT FROM AUTHOR]

Background: Integrated school and home garden interventions can improve health outcomes in low-income countries, but rigorous evidence remains scarce, particularly for school-aged children and to reduce anemia. Objective: We test if an integrated school and home garden intervention, implemented at pilot stage, improves hemoglobin levels among school children (aged 9-13 years) in a rural district in the mid-hills of Nepal. Methods: We use a cluster randomized controlled trial with 15 schools each in the control and treatment groups (n = 680 school children). To test if nutritional improvements translate into a reduction of anemia prevalence, hemoglobin data were collected 6 months after intervention support had ended. Using structural equation modeling, we estimate the direct and indirect effects of the treatment through several pathways, including nutritional knowledge, good food and hygiene practices, and dietary diversity. Results: The integrated school and home garden intervention did not lead to a direct significant reduction in anemia. Causal positive changes of the treatment on nutritional outcomes, although significant, are not strong enough to impact hemoglobin levels. The program improved hemoglobin levels indirectly for children below 12 by increasing the use of good food and hygiene practices at home. These practices are associated with higher hemoglobin levels, particularly for girls, young children, and in households where caregivers are literate. Conclusions: Even integrated school and home garden interventions are not sufficient to reduce anemia among school children. Incorporating behavioral change components around food and hygiene practices into integrated garden interventions is important to unlocking their health impacts. Plain language title: Effect of a Joint School and Home Garden Programme on Child Health Among School-Aged Children in Nepal Plain language summary: The effects of agricultural and nutritional support programs on health are not fully understood. This article looks at school and home gardens, which are gaining attention in the research and development community, but evidence for their effects is limited, particularly for lower-income countries. We examine whether a joint school and home garden program improves blood iron levels among school children (aged 9-13 years) in rural Nepal. Low levels are an indicator for anemia—a common nutritional disorder in South Asia. Our data come from an experiment involving 30 schools and 680 children and was measured via a finger prick method 6 months after program support ended. We also evaluate if the program influenced blood iron levels through the nutritional knowledge of children and their caregivers; through the use of good food and hygiene practices at home and the children's dietary diversity. Our results indicate that the program did not lead to a direct reduction in anemia. However, for children under the age of 12, the program indirectly improved blood iron levels through behavioral changes in the use of good food and hygiene practices (e.g., washing hands before eating). We therefore argue that including education on nutrition and good food and hygiene practices as part of integrated garden support programs is key to improving children's health. [ABSTRACT FROM AUTHOR]

Farmers in sub-Saharan Africa do not have much choice of quality seed of vegetable cultivars adapted to local growing conditions and consumer demand. Only a handful of vegetable seed companies invest in local breeding research, while nearly all rely on seed imports. Our objective was to analyse to what extent existing seed laws and regulations enable or restrict the development of a research-based vegetable seed sector in Africa. Using a regulatory value chain approach, we analysed written laws and regulations for 13 countries and interviewed private and public sector stakeholders. We generally find that countries have taken a government-centred approach to seed quality control without recognizing that government agencies have little capacity to do this properly for vegetable crops. Mandatory Value for Cultivation and Use (VCU) trials and state-controlled seed certification are the two examples of regulatory approaches that may work for cereals but are not well suited for vegetables. No country has vegetable breeding as a goal in their national seed policy, and only four set out specific objectives for the vegetable seed sector. Tailoring seed policies, laws and regulations more specifically to vegetables could stimulate local variety development and seed production and benefit smallholder farmers and consumers at large. [ABSTRACT FROM AUTHOR]

India experienced a rapid rise in COVID-19 infections from March 2021. States imposed varying levels of lockdowns and curfews to curb the spread of the disease. These restrictions severely affected the functioning of food systems. The objective of this study was to analyze how COVID-19 continues to affect agricultural production, food security and household diets of vegetable farmers. A phone-based survey was conducted with 595 vegetable farmers in the states of Andhra Pradesh, Assam, Jharkhand, Karnataka and Odisha, 60% of whom had been interviewed a year earlier. Overall, 60% of farmers experienced decreased vegetable production; over 80% reported a reduction in consumption of at least one food group; and 45% reported some level of food insecurity between May 2020 and May 2021. Farmers who reported decreased staples production, difficulty accessing seeds/seedlings, or reduced their household spending were more likely to report decreased vegetable production. Vegetable consumption was positively associated with receipt of COVID-19 relief benefits, borrowing money, or having home gardens. Farmers who received public agricultural assistance, or had reduced expenses, were more likely to have lower vegetable consumption. Greater severity of food insecurity was associated with farmers belonging to underprivileged social groups, non-Hindus, or those who experienced decrease in livestock production, weather related disruptions or received COVID-19 assistance. This is one of few studies that have conducted a longitudinal assessment of the impacts across multiple waves of COVID-19. COVID-19 is seen to be one among several shocks experienced by farm households, and exacerbated existing issues within agriculture and food security. There is a need for public policy support to strengthen both production and consumption of vegetables. [ABSTRACT FROM AUTHOR]