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The flexibility in growth of Betula pubescens is taken as example for possible erroneous imaginations of Late Glacial–Early Holocene living conditions. The article highlights the factors influencing the phenotype of the contemporary plants, as well as the small-scale patchiness of the distribution of the vegetation in the landscape. The area of investigation is the southern Weichselian younger morainic area of Schleswig-Holstein, including a major Late Glacial archaeological type locality – the Ahrensburg tunnel valley. The period under investigation spans the time between 11 700 and 11 400 cal BP and hence the Late Glacial–Early Holocene transition, which is characterized by a significant shift in the vegetation. This shift has often been seen as a simple transition from treeless tundra to birch forest. The aim of the article is to provide a more realistic image of plant cover of the late Dryas 3 period and the Early Holocene period before the Preboreal oscillation by considering past natural growth limits of Betula pubescens in the study area. The intention is to provide a more accessible interpretation of palaeobotanical data to non-specialists, especially in archaeology.

The optimisation of the power output of wind turbines requires the consideration of various aspects including turbine design, wind farm layout and more. An improved understanding of the interaction of wind turbines with the atmospheric boundary layer is an essential prerequisite for such optimisations. With numerical simulations, a variety of different situations and turbine designs can be compared and evaluated. For such a detailed analysis, the output of an extensive number of turbine and flow parameters is of great importance. In this paper a coupling of the aeroelastic code FAST (fatigue, aerodynamics, structures, and turbulence) and the large-eddy simulation tool PALM (parallelised large-eddy simulation model) is presented. The advantage of the coupling of these models is that it enables the analysis of the turbine behaviour, among others turbine power, blade and tower loads, under different atmospheric conditions. The proposed coupling is tested with the generic National Renewable Energy Laboratory (NREL) 5 MW turbine and the operational eno114 3.5 MW turbine. Simulating the NREL 5 MW turbine allows for a first evaluation of our PALM–FAST coupling approach based on characteristics of the NREL turbine reported in the literature. The basic test of the coupling with the NREL 5 MW turbine shows that the power curve obtained is very close to the one when using FAST alone. Furthermore, a validation with free-field measurement data for the eno114 3.5 MW turbine for a site in northern Germany is performed. The results show a good agreement with the free-field measurement data. Additionally, our coupling offers an enormous reduction of the computing time in comparison to an actuator line model, in one of our cases by 89 %, and at the same time an extensive output of turbine data.

Organic matter production by cyanobacteria blooms is a major environmental concern for the Baltic Sea, as it promotes the spread of anoxic zones. Partial pressure of carbon dioxide (pCO2) measurements carried out on Ships of Opportunity (SOOP) since 2003 have proven to be a powerful tool to resolve the carbon dynamics of the blooms in space and time. However, SOOP measurements lack the possibility to directly constrain depth-integrated net community production (NCP) in moles of carbon per surface area due to their restriction to the sea surface. This study tackles the knowledge gap through (1) providing an NCP best guess for an individual cyanobacteria bloom based on repeated profiling measurements of pCO2 and (2) establishing an algorithm to accurately reconstruct depth-integrated NCP from surface pCO2 observations in combination with modelled temperature profiles. Goal (1) was achieved by deploying state-of-the-art sensor technology from a small-scale sailing vessel. The low-cost and flexible platform enabled observations covering an entire bloom event that occurred in July–August 2018 in the Eastern Gotland Sea. For the biogeochemical interpretation, recorded pCO2 profiles were converted to CT*, which is the dissolved inorganic carbon concentration normalised to alkalinity. We found that the investigated bloom event was dominated by Nodularia and had many biogeochemical characteristics in common with blooms in previous years. In particular, it lasted for about 3 weeks, caused a CT* drawdown of 90 µmol kg−1, and was accompanied by a sea surface temperature increase of 10 ∘C. The novel finding of this study is the vertical extension of the CT* drawdown up to the compensation depth located at around 12 m. Integration of the CT* drawdown across this depth and correction for vertical fluxes leads to an NCP best guess of ∼1.2 mol m−2 over the productive period. Addressing goal (2), we combined modelled hydrographical profiles with surface pCO2 observations recorded by SOOP Finnmaid within the study area. Introducing the temperature penetration depth (TPD) as a new parameter to integrate SOOP observations across depth, we achieve an NCP reconstruction that agrees to the best guess within 10 %, which is considerably better than the reconstruction based on a classical mixed-layer depth constraint. Applying the TPD approach to almost 2 decades of surface pCO2 observations available for the Baltic Sea bears the potential to provide new insights into the control and long-term trends of cyanobacteria NCP. This understanding is key for an effective design and monitoring of conservation measures aiming at a Good Environmental Status of the Baltic Sea.

Marginal seas are directly subjected to anthropogenic and natural influences from land in addition to receiving inputs from the atmosphere and open ocean. Together these lead to pronounced gradients and strong dynamic changes. However, in the case of mercury emissions from these seas, estimates often fail to adequately account for the spatial and temporal variability of the elemental mercury concentration in surface water (Hg0wat). In this study, a method to measure Hg0wat at high resolution was devised and subsequently validated. The better-resolved Hg0wat dataset, consisting of about one measurement per nautical mile, yielded insight into the sea's small-scale variability and thus improved the quantification of the sea's Hg0 emission. This is important because global marine Hg0 emissions constitute a major source of atmospheric mercury. Research campaigns in the Baltic Sea were carried out between 2011 and 2015 during which Hg0 both in surface water and in ambient air were measured. For the former, two types of equilibrators were used. A membrane equilibrator enabled continuous equilibration and a bottle equilibrator assured that equilibrium was reached for validation. The measurements were combined with data obtained in the Baltic Sea in 2006 from a bottle equilibrator only. The Hg0 sea–air flux was newly calculated with the combined dataset based on current knowledge of the Hg0 Schmidt number, Henry's law constant, and a widely used gas exchange transfer velocity parameterization. By using a newly developed pump–CTD with increased pumping capability in the Hg0 equilibrator measurements, Hg0wat could also be characterized in deeper water layers. A process study carried out near the Swedish island Øland in August 2015 showed that the upwelling of Hg0-depleted water contributed to Hg0 emissions of the Baltic Sea. However, a delay of a few days after contact between the upwelled water and light was apparently necessary before the biotic and abiotic transformations of ionic to volatile Hg0 produced a distinct sea–air Hg0 concentration gradient. This study clearly showed spatial, seasonal, and interannual variability in the Hg0 sea–air flux of the Baltic Sea. The average annual Hg0 emission was 0.90 ± 0.18 Mg for the Baltic proper and extrapolated to 1.73 ± 0.32 Mg for the entire Baltic Sea, which is about half the amount entrained by atmospheric deposition. A comparison of our results with the Hg0 sea–air fluxes determined in the Mediterranean Sea and in marginal seas in East Asia were to some extent similar but they partly differed in terms of the deviations in the amount and seasonality of the flux.

Die Analyse zweier Bohrkerne von Grund der Nordsee und dem in Analogie mit Doggerland bezeichneten ehemaligen Nordseefestland boten die Möglichkeit eines besseren Verständnisses pleistozäner und frühholozäner Vegetationsgeschichte. Basierend auf hochauflösenden Pollendiagrammen an Basalen Torfen wurden Vegetationsentwicklungen und Umweltbedingungen untersucht. Die Diagramme zeigen, dass sich die Mitteleuropäische Grundsukzession in weiten Teilen nachverfolgen lässt. Eine Bestimmung der Holzkohlekonzentrationen wurde vorgenommen, jedoch konnten keine sicheren Nachweise für menschliche Aktivität erbracht werden.

In geological outcrops and drill cores from reef frameworks, the skeletons of scleractinian corals are usually leached and more or less completely transformed into sparry calcite because the highly porous skeletons formed of metastable aragonite (CaCO3) undergo rapid diagenetic alteration. Upon alteration, ghost structures of the distinct annual growth bands often allow for reconstructions of annual extension ( = growth) rates, but information on skeletal density needed for reconstructions of calcification rates is invariably lost. This report presents the bulk density, extension rates and calcification rates of fossil reef corals which underwent minor diagenetic alteration only. The corals derive from unlithified shallow water carbonates of the Florida platform (south-eastern USA), which formed during four interglacial sea level highstands dated approximately 3.2, 2.9, 1.8, and 1.2 Ma in the mid-Pliocene to early Pleistocene. With regard to the preservation, the coral skeletons display smooth growth surfaces with minor volumes of marine aragonite cement within intra-skeletal porosity. Within the skeletal structures, voids are commonly present along centres of calcification which lack secondary cements. Mean extension rates were 0.44 ± 0.19 cm yr−1 (range 0.16 to 0.86 cm yr−1), mean bulk density was 0.96 ± 0.36 g cm−3 (range 0.55 to 1.83 g cm−3) and calcification rates ranged from 0.18 to 0.82 g cm−2 yr−1 (mean 0.38 ± 0.16 g cm−2 yr−1), values which are 50 % of modern shallow-water reef corals. To understand the possible mechanisms behind these low calcification rates, we compared the fossil calcification rates with those of modern zooxanthellate corals (z corals) from the Western Atlantic (WA) and Indo-Pacific calibrated against sea surface temperature (SST). In the fossil data, we found a widely analogous relationship with SST in z corals from the WA, i.e. density increases and extension rate decreases with increasing SST, but over a significantly larger temperature window during the Plio-Pleistocene. With regard to the environment of coral growth, stable isotope proxy data from the fossil corals and the overall structure of the ancient shallow marine communities are consistent with a well-mixed, open marine environment similar to the present-day Florida Reef Tract, but variably affected by intermittent upwelling. Upwelling along the platform may explain low rates of reef coral calcification and inorganic cementation, but is too localised to account also for low extension rates of Pliocene z corals throughout the tropical WA region. Low aragonite saturation on a more global scale in response to rapid glacial–interglacial CO2 cyclicity is also a potential factor, but Plio-Pleistocene atmospheric pCO2 is generally believed to have been broadly similar to the present day. Heat stress related to globally high interglacial SST only episodically moderated by intermittent upwelling affecting the Florida platform seems to be another likely reason for low calcification rates. From these observations we suggest some present coral reef systems to be endangered from future ocean warming.

Clay mineral assemblages in a sediment core from the distal Nile discharge plume off Israel have been used to reconstruct the late Quaternary Nile sediment discharge into the eastern Mediterranean Sea (EMS). The record spans the last ca. 140 kyr. Smectite abundances indicate the influence of the Blue Nile and the Atbara River that have their headwaters in the volcanic rocks of the Ethiopian Highlands. Kaolinite abundances indicate the influence of wadis, which contribute periodically to the suspension load of the Nile. Due to the geographical position, the climate and the sedimentary framework of the EMS is controlled by two climate systems. The long-term climate regime was governed by the African monsoon that caused major African humid periods (AHPs) with enhanced sediment discharge at 132 to 2 kyr), S4 (3.5 kyr), and S3 (5 kyr). During the last glacial period (Marine Isotope Stages (MISs) 4–2), the long-term changes in the monsoonal system were superimposed by millennial-scale changes in an intensified midlatitude glacial system. This climate regime caused short but pronounced drought periods in the Nile catchment, which are linked to Heinrich events and alternate with more humid interstadials. The clay mineral record further implies that feedback mechanisms between vegetation cover and sediment discharge of the Nile are detectable but of minor importance for the sedimentary record in the southeastern Mediterranean Sea during the investigated African humid periods.

The fast growing calcareous skeletons of zooxanthellate reef corals (z corals) represent unique environmental proxy archives through their oxygen and carbon stable isotope composition (δ18O, δ13C). In addition, the accretion of the skeleton itself is ultimately linked to the environment and responds with variable growth rates (extension rate) and density to environmental changes. Here we present classical proxy data (δ18O, δ13C) in combination with calcification records from 15 massive z corals. The z corals were sampled from four interglacial units of the Florida carbonate platform (USA) dated approximately 3.2, 2.9, 1.8 and 1.2 Ma (middle Pliocene to early Pleistocene). The z corals (Solenastrea, Orbicella, Porites) derive from unlithified shallow marine carbonates and were carefully screened for primary preservation suited for proxy analysis. We show that skeletal accretion responded with decreasing overall calcification rates (decreasing extension rate but increasing density) to warmer water temperatures. Under high annual water temperatures, inferred from sub-annually resolved δ18O data, skeletal bulk density was high, but extension rates and overall calcification rates were at a minimum (endmember scenario 1). Maximum skeletal density was reached during the summer season giving rise to a growth band of high density within the annually banded skeletons (“high density band”, HDB). With low mean annual water temperatures (endmember scenario 2), bulk skeletal density was low but extension rates and calcification rates reached a maximum, and under these conditions the HDB formed during winter. Although surface water temperatures in the Western Atlantic warm pool during the interglacials of the late Neogene were ∼ 2 °C higher than they are in the present day, intermittent upwelling of cool, nutrient-rich water mitigated water temperatures off south-western Florida and created temporary refuges for z coral growth. Based on the sub-annually resolved δ18O and δ13C records, the duration of the upwelling episodes causing the endmember 2 conditions was variable and lasted from a few years to a number of decades. The episodes of upwelling were interrupted by phases without upwelling (endmember 1) which lasted for at least a few years and led to high surface water temperatures. This variable environment is likely one of the reasons why the coral fauna is dominated by the eurytopic genus Solenastrea, also a genus resistant to high turbidity. Over a period of ∼ 50 years, the oldest sub annually resolved proxy record available (3.2 Ma) documents a persistent occurrence of the HDB during winter. In contrast, the HDB forms in summer in modern z corals from the Florida reef tract. We suggest this difference should be tested as being the expression of a tendency towards decreasing interglacial upwelling since the middle Pliocene. The number of z coral sclerochronological records for the Plio-Pleistocene is still rather low, however, and requires more data and an improved resolution, through records from additional time slices. Nonetheless, our calcification data from the warm periods of past interglacials may contribute to predicting the effects of future ocean warming on z coral health along the Florida reef tract. The inconsistent timing of the HDB within single coral records or among specimens and time slices is unexpected and contrasts the common practice of establishing chronologies on the basis of the density banding.

Fe isotope ratios and concentrations of dissolved Fe (Fedis, < 0.45 μm) and of suspended particulate Fe (FeSPM) were analyzed from a depth profile through the anoxic Eastern Gotland Basin water column, Baltic Sea. Results show a sharp gradient in δ56Fedis across the ferruginous layer with δ56Fedis = −0.4‰ in the euxinic deep basin and δ56Fedis = &plus;0.3‰ in the oxic upper water column. The isotopic gradient overlaps with a strong concentration gradient of Fedis, a concentration maximum in FeSPM and lower δ56FeSPM values than δ56Fedis. These features indicate preferential loss of light Fe isotopes from solution to suspended iron-oxyhydroxides (FeIOH) during typical oxidative precipitation across the redox interface. The sign of the overall fractionation, Δ56FeIOH-Fe(II)(aq) < 0‰, is in contrast to similar, mostly non-marine redox environments, where Δ56FeIOH-Fe(II)(aq) > 0‰. The difference appears to be the result of isotope exchange dominated by reaction kinetics in the marine water column, rather than equilibrium fractionation generally inferred for oxidative Fe precipitation elsewhere. High residual δ56Fedis immediately above the oxic–ferruginous interface and throughout the oxic water column suggests that any potential dissolved Fe export from marine reducing waters into the oxic open water column is enriched in the heavy isotopes. In the deep, mildly euxinic water column above the level of Fe sulfide saturation, a decreasing δ56FeSPM trend with depth and a generally low δ56Fedis are comparable to trends generally observed in marine anoxic sediment profiles where microbial reductive Fe dissolution occurs. The isotope composition of the redox-cycled Fe inventory in anoxic marine basins mainly reflects the balance between external fluxes, driving the composition towards crustal δ56Fe values, and intensity of internal recycling, driving δ56Fe towards negative values.

The conception of the manufacturing process to be initially applied in the parts rapid production targeting a first embodiment of an idea (prototypes) and without many demands on strength and accuracy is called rapid prototyping. New rapid prototyping technologies are more accessible under the aspects of cost, accuracy, usability, environmental and prototypes started to be increasingly explored in the sustainable product development process. This paper proposes the application of hierarchical analysis method to support the decision-making in Rapid Prototyping processes based on the analysis of product and RP technology and prototype characteristics. The application of the developed method consists of assigning numeric values to the product characteristics and to the important characteristics for users in order to build the comparison matrix to perform the assessment and the prioritization steps of the relevant features. Performing the prioritization of features considered most important along with the characteristics of the chosen prototyping technology make possible the analysis of the effect of the characteristics prioritization. The concepts of product development, rapid prototyping and analytic hierarchy process for the sustainable product were used in a structure (lightweight dual) to evaluate the proposed strategy. The lightweight dual product from the 3D printing technology has been successful to show the product’s purpose and functionality due to its lower cost, short execution time, good surface finishing and dimensional accuracy. The results show that the analysis of the sustainable product for rapid prototyping technology based on characteristics prioritization strategy improves the product design.

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Background: Lactose intolerance with adult-onset is due to the inadequate enzymatic activity of lactase-phlorizin hydrolase (LPH). It is frequently seen in patients with Crohn disease, but the mechanism remains to be elucidated. Two DNA genotypes, C/C -13910 and G/G -22018 , located upstream from the LCT locus, the gene encoding for LPH, were recently identified as representing genetic markers for lactose intolerance. We utilized these two DNA genotypes to study their role in inflammatory bowel disease. Methods: We investigated the prevalence of these two DNA variants using specific restriction enzyme digest assays in 166 patients with Crohn disease, in 120 healthy first-degree relatives of Crohn disease patients, in 63 patients with ulcerative colitis and in 187 healthy individuals. Results: The analysis revealed a frequency of 21.4% of the 2 genotypes for adult-type hypolactasia in our studied German cohort of healthy individuals, which is higher than previously reported (15%) based on the hydrogen (H 2 ) breath test. This might indicate a higher sensitivity of genotyping, but it has to be confirmed in larger cohorts. No significant difference was detectable in the frequency of the C/C -13910 and G/G -22018 genotypes in patients with Crohn disease (C/C -13910 : 21.7%; G/G -22018 : 22.3%) compared to first-degree relatives (C/C -13910 : 21.7%; G/G -22018 : 20.8%), patients with ulcerative colitis (C/C -13910 : 20.3%; G/G -22018 : 20.3%) and healthy individuals (C/C -13910 : 21.4%; G/G -22018 : 21.4%). Conclusion: The C/C -13910 and G/G -22018 genotype of adult-type hypolactasia is not associated with susceptibility to the pathogenesis of Crohn disease and ulcerative colitis. [ABSTRACT FROM AUTHOR]

Inherited susceptibility to breast cancer is associated with an early onset and bilateral disease. The extent of familial risks has not, however, been fully assessed in population-based incidence studies. The purpose of the study was to quantify the risks for cancers of the breast, ovary and other sites of close relatives of women in whom breast cancer was diagnosed at an early age. Records collected between 1943 and 1990 at the Danish Cancer Registry were searched, and 2860 women were found in whom breast cancer was diagnosed before age 40. Population registers and parish records were used to identify 14 973 parents, siblings and offspring of these women. Cancer occurrence through to 31 December 1993 was determined within the Cancer Registry's files and compared with national incidence rates. Women with early-onset breast cancer were at a nearly fourfold increased risk of developing a new cancer later in life (268 observed vs 68.9 expected). The excess risk was most evident for second cancer of the breast (181 vs 24.5) and for ovarian cancer (20 vs 3.3). For mothers and sisters, risks for cancers of the breast and ovary were significantly increased by two- to threefold. Bilateral breast cancer and breast-ovarian cancer were very strong predictors of familial risks, with one in four female relatives predicted to develop breast and/or ovarian cancer by age 75. Mothers had a slightly increased risk of colon cancer, but not endometrial cancer. The risk for breast cancer was also increased among fathers (standardized incidence ratio 2.5; 95% CI 0.5-7.4) and especially brothers (29; 7.7-74), although based on small numbers. The risk for prostatic cancer was unremarkable. In this large population-based survey, the first-degree relatives of women who developed breast cancer before age 40 were prone to ovarian cancer as well as male and female breast cancer, but not other tumours that may share susceptibility genes with breast cancer. [ABSTRACT FROM AUTHOR]

A major task of the newly established "Southern African Science Service Centre for Climate Change and Adaptive Land Management" (SASSCAL; www.sasscal.org) and its partners is to provide science-based environmental information and knowledge which includes the provision of consistent and reliable climate data for Southern Africa. Hence, SASSCAL, in close cooperation with the national weather authorities of Angola, Botswana, Germany and Zambia as well as partner institutions in Namibia and South Africa, supports the extension of the regional meteorological observation network and the improvement of the climate archives at national level. With the ongoing rehabilitation of existing weather stations and the new installation of fully automated weather stations (AWS), altogether 105 AWS currently provide a set of climate variables at 15, 30 and 60 min intervals respectively. These records are made available through the SASSCAL WeatherNet, an online platform providing near-real time data as well as various statistics and graphics, all in open access. This effort is complemented by the harmonization and improvement of climate data management concepts at the national weather authorities, capacity building activities and an extension of the data bases with historical climate data which are still available from different sources. These activities are performed through cooperation between regional and German institutions and will provide important information for climate service related activities.

Heritage events serve as a way for enthusiasts to come together and celebrate their shared heritage interests. The Sandstone Estate is home to one of the largest collections of functioning vintage agricultural, railway and military artefacts in private ownership. Amos was used to provide statistical support for the Structural Equation Model. The data provided support for the Structural Equation Model, which produced an acceptable model fit. The Structural Equation Model indicates that travel motives, life domains and life domains overall have an influence on tourist’s Quality of Life attending a heritage event. Travel motives, life domains such as: travel life, social life, leisure and recreation life, self-life, life domains overall and Quality of Life attained acceptable reliabilities. The purpose of this study is to examine whether travel motives and life domains have an influence on tourists’ QoL attending a heritage event. Permission was given by management to send a cover letter explaining the purpose of the study. Tourists had been asked to leave their contact emails at free will with the estate to participate in this study. A link to a self-administrated online questionnaire was attached to the e-mail for tourists who chose to participate anonymously in this study. Data from 119 (N) completed questionnaires were captured and analysed using SPSS. From the study`s findings, managerial recommendations were made with the aim of maximising attending heritage tourists` Quality of Life. The research contributes to literature of tourism and QoL.

We report the case of a 55-year-old male patient who presented with non-specific pulmonary symptoms (cough, haemoptysis, fever up to 39 degrees C, night sweats and weight loss). After empirical antibiotic therapy prescribed by his primary care physician, the patient showed no improvement in symptoms. Laboratory findings were: elevated C-reactive protein and C-ANCA, leukocytosis and thrombocytosis, and anaemia. Chest radiography showed disseminated nodules bilaterally. On multidetector-row computed tomography (MDCT), the bronchial walls showed a significant thickening and extensive peribronchiolar consolidations. Bronchoscopy revealed diffuse erythema of the tracheobronchial mucosa with diffusely scattered white plaques. Histopathology described a multifocal ulcerative bronchitis with underlying chronic bronchitis. These findings in combination with the laboratory data lead to the diagnosis of Wegener's granulomatosis. Consequently, we started with an immunosuppressive therapy. Chest radiography after 10 days showed marked resolution of the infiltrates. Within 1 month, the patient became asymptomatic. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this paper is to measure tourists' satisfaction with quality of life issues at an arts festival - the Aardklop National Arts Festival in Potchefstroom. Problem investigated: Understanding satisfaction with quality of life issues could assist Festival organizers and other businesses to improve services for tourists by tailoring these to meet their needs. Satisfied tourists spread positive word-of-mouth communication and are more likely to return in the future. Methodology: A self-administered survey developed and validated by Neal, Sirgy and Uysal (1999:156 & 2004:245) was fielded at the 2007 Aardklop National Arts Festival in Potchefstroom. The target population included all visitors to the Festival who were staying for two or more days, and who had travelled to Potchefstroom from elsewhere. Findings and implications: Respondents were satisfied with travel / tourism services and experiences at the Festival, and with their leisure time and life in general. However, certain things at the Festival led to lowered levels of satisfaction. Demographically, respondents did not differ significantly in their levels of satisfaction with travel / tourism services, travel / tourism experiences, leisure life, and life in general at the Festival except in terms of population group and region from which they came. Organizers of the Festival and other businesses should take cognizance of the fact that all parties involved in providing services to tourists attending an arts festival impact on their satisfaction levels with travel / tourism services and experiences - and ultimately also on their quality of life. Tourists attending the arts festival are not homogenous and differ substantially in terms of their demographic profile. Tourist service providers should tailor their offerings in order to satisfy the varied needs of the different types of tourist attending the Festival. Originality and value of the research: This research focused on measuring tourists' satisfaction with quality of life issues at an arts festival. Such research has never before been conducted in South Africa; the results contribute to the deeper insight of interested parties (such as the tourism industry and arts festival managers) into what they need to offer at such events.

Notwithstanding family businesses being a prevalent phenomenon in the economies of most countries, insight into this form of enterprise is still limited and it has been largely ignored as a study field. Interest has now grown in identifying and understanding those facets of business that support the superior performance of family firms. As interest in the field of family business has expanded, so have the theories that support these views. Theory therefore provides a guideline to investigate causalities and link information. It indicate that presently there is no single, generally accepted theory defining the family-firm concept and that rigorous work on such theory is only just starting. The purpose of this article is to review the different theories underlying family businesses. It is concluded that the orthodox theories, that regard the business and the family as separate units, do not acknowledge the complexity of the different relationships that exist in family businesses. A Conceptual Familiness* Transmission of Capital Model is suggested in the current study as representative of the functioning of family businesses as an interactive system. It embraces the heterodox views that the family and the business cannot be separated, but are rather seen as an interactive system with unique, collectable resources. It fulfils the need of the main deficiency in the family organisation literature, namely a theory that explains the developmental stages of each generation succession. *Used in the context of Habbershon and Williams (1999:1)

We investigate properties of algorithms that are used to solve coupled evolutionary partial differential equations posed on neighboring, nonoverlapping domains, where the solutions are coupled by continuity of state and normal flux through a shared boundary. The algorithms considered are based on the widely used approach of iteratively exchanging boundary condition data on the shared boundary at each time step. There exists a significant and sophisticated numerical analysis of such methods. However, computations for practical applications are often carried out under conditions under which it is unclear if rigorous results apply while relatively few iterations are used per time step. To examine this situation, we derive exact matrix expressions for the propagation of the error due to incomplete iteration that can be readily evaluated for specific discretization parameters. Using the formulas, we show that the universal validity of several tenants of the practitioner’s conventional wisdom are not universally valid.

Purpose: This paper describes the implementation of and lessons learnt with an action research project on management teaching and learning in a 21st century transitional university. The project focuses on the problem of how to elicit and shape students' tacit knowing for meta-innovation and is part of a drive to find a new identity for the newly merged comprehensive University of Johannesburg (UJ). Design/methodology/approach: The project under discussion focuses on an undergraduate module, Developing and Managing Innovation, presented by UJ since 2003 as part of the B.Com Intrapreneurial Management degree. This degree has been developed in the light of the recent requirements placed upon managers by the innovation era. Creating new knowledge is not simply a matter of processing objective information but rather of tapping tacit and often highly subjective insights, intuitions and hunches. To find ways to elicit and shape tacit knowing for meta-innovation, an extensive literature study was conducted and a model identified for this purpose. An action research spiral was constructed to validate the teaching and learning interventions. Findings: The paper presents a teaching and learning framework to build theory that is in accord with the African Ubuntu spirit. The framework supports students within powerful learning environments to develop meta-cognition skills by focusing not only on the acquisition of explicit knowledge, but also on ways to elicit and shape tacit knowing. Implications: A community of practice is the bedrock of powerful learning environments in which action and learning, improvisation and experimentation, tacit and explicit knowledge feed on each other to stretch the students' capacity for meta-innovation. This enables them to continually deploy their talents, knowledge, resourcefulness and creativity to best effect as managers and to transform their life and that of their business and of others. Originality/value: The innovative curriculum and instructional design model generated in this project will assist UJ and other universities in transition to become engaged 21st century universities of excellence that can meet the societal, cultural and economic needs and interests of the national transformative agenda, aimed at shared growth and wealth in Africa.

While family businesses are known to consistently outperform non-family businesses in financial terms over the long run, family businesses have received comparatively little attention from researchers. In this article an explanation is offered for this superior performance in the form of the concept of "familiness" - the unique contribution that family involvement brings to any business (which is divided into founder capital and family capital). It is explained that family businesses possess no general competitive advantage over non-family businesses. The unique strength of successful family businesses does not lie in their espoused advantages, but in their ability to sustain and adapt, through family capital, the culture created by the founder. An evolutionary conceptual model of the creation and transmission of familiness is provided to explain how this unique strength influences family business performance over the long run.

South African organisations must undergo a mind shift and adhere to certain prerequisites to survive and be successful. It is evident that companies not changing their mindsets will not survive and be able to create a sustainable competitive advantage and to compete in world markets. Companies have to solve new problems with new paradigms, constantly create something better, something new, create new markets as opposed to increasing market share. The Third Wave development will lead to societal transformation. Moving to Third Wave will imply growth organisations to act like small entrepreneurial businesses that will have the benefit of speed and simplicity but also be able to implement strategy more effectively. Time is of the essence. South African companies have no other option but to move swiftly. The transformation from second to third wave is inevitable.

This paper describes a study aimed at increasing an organisation's ability to implement a strategy for establishing an intrapreneurial orientation effectively. Establishing an intrapreneurial orientation will be treated from a strategic management point of view, with the emphasis on the implementation phase of strategic management. As such, this study seeks to integrate theory and practice from the fields of strategic management and entrepreneurship.

No abstract available.

It has been shown that large numbers of low-income SA households use wood for domestic cooking and spaceheating purposes and are exposed to high concentrations of air pollutants emitted from the unsophisticated appliances being utilised. Although the related problem of exposure to coal smoke from domestic fires has been and is being addressed, wood smoke exposure remains a pressing problem. The design of a more efficient wood using appliance therefore has the potential to reduce the particulate matter exposure of a considerable portion of the SA population. This paper presents the design of a natural draft wood gasification stove for domestic use based on the inverted downdraft principle. Prototypes have been built in two sizes, suitable for relatively unsophisticated manufacturing techniques. The results of performance testing as well emission factor determination are presented. It is shown that simple wood gasification stoves can at least be used for cooking, with a considerable reduction in exposure of household members to particulate matter inhalation.

Business ethics in business training: Oratory or the actuality. This article is the culmination of an in-depth literature study. On the one hand an attempt is made to incorporate the views of different authors, while on the other hand an attempt is made to take part in the debate which is initiated by the current renewal of interest in the subject Business Ethics. Within this framework attention is paid to the question of whether business ethics can be taught and if so, to what extent it's influence will be felt. Secondly, an insight into the teaching of business ethics in the future is provided. Within this context the approach to the teaching, the content, the role of the student and the responsibility of the educator in particular are addressed. Opsomming Hierdie artikel is die resultaat van 'n indringende literatuurstudie. Daar word gepoog om enersyds verskillende skrywers se standpunte saam te vat, maar andersyds ook kritiese kommentaar te lower en deel te neem aan die debat wat deur die huidige opiewing in die belangstelling in Bestuursetiek bestaan. Binne die raamwerk sal aandag aan die volgende geskenk word: Die beantwoording van die vraag of Bestuursetiek onderrig kan word en indien wel die trefwydte daarvan. Tweedens 'n toekomsblik op die onderrig van Bestuursetiek. Binne die konteks word die benadering tot die onderrig/ die inhoud en die rol van die student en die verantwoordelikheid van die dosent bekvk.

Third Wave management: A deliberation on the principles The postmodern understanding of reality revolves around the idea that there are no absolute values - everything is in flux. Postmodernism is a revolutionary epoch in man's existence and implies a transitionary state from modernism to a new era of thinking. The science of management, being dynamic, is continually interpreting the status of presentday companies. Toffler proposed a managerial theory called Third Wave management. This theory embodies the current postmodern understanding of reality. While a superficial similarity exists between Third Wave management and the New Age Movement, it does not necessarily mean that Third Wave management shares the ideological and spiritual presuppositions of the New Age Movement. Third Wave management theory can be interpreted as part of a post-modernist paradigm which eventually precipitates in middle range management science (a mesoparadigm). Several managerial models and structures can thus be developed.

Affirmative action: an ethical perspective. The scrapping of certain relevant laws of the apartheid era can not immediately undo the wrongs of apartheid. What is essentially needed is compensation of some kind for every individual or group who has been affected by it. The question is whether affirmative action can be ethically justified; is it inherently 'right or wrong’? In this paper different approaches to affirmative action and the normal phases of implementation of affirmative action are investigated. The best known ethical theories are then used to adjudicate the relevance ami ethical justification of affirmative action. In the final analysis six different levels of ethical decision-making are considered. The conclusion arrived at is that affirmative action is unavoidable but that there are a number o f pitfalls awaiting the unwary. To implement affirmative action successfully certain prerequisites must be met.

The pursuit of high productivity is not a goal in itself, but rather regulates the attempt to raise a country's standard of living. Since the standard of living is dependent on the relationship between the total output and input of all businesses, the productivity of a single business has a great influence. Increasing productivity is considered to be an extremely important management function. I f productivity is regarded as the measuring instrument of successful management, then the management process and, more specifically, management by objectives is the method for achieving this. It is management’s responsibility to create the necessary climate of productivity and increased productivity. The approach must be one which achieves a balance between task-orientation and people-orientation. Consequently the purpose and meaning of work should also be reconsidered. Work is not a goal in itself but must always be seen within the wider context of God’s cultural mandate to man. When everyone understands the meaning of work we will emerge from the so-called ‘reward psychosis’, and reward will be offered according to need and achievement. Reward and work fulfilment will lead to the creation of a work environment which is meaningful of the worker, which promotes increased productivity, and which allows self-actualization toflourish. Management is capable of creating and maintaining these circumstances which will increase productivity.

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Unsere zunehmende Abhängigkeit von Informationstechnik erhöht kontinuierlich die Safety- und Security-Anforderungen bei deren Einsatz. Ein zentrales Problem hierbei sind Schwachstellen von Hard- und Software. Marktkräfte konnten diese Situation bislang nicht grundsätzlich beheben. Eine Gegenstrategie sollte deshalb folgende Optionen erwägen: (1) private und staatliche Förderung offener und sicherer IT‑Produktion, (2) Verbesserung der souveränen Kontrolle bei der Produktion aller kritischen IT‑Komponenten innerhalb eines Wirtschaftsraumes sowie (3) verbesserte und durchgesetzte Regulierung. Dieser Beitrag analysiert Vor- und Nachteile dieser Optionen. Es wird vorgeschlagen, die Sicherheit der Schlüsselkomponenten einer Lieferkette durch weltweit verteilte, offene und ggf. mathematisch bewiesene Komponenten zu gewährleisten. Der beschriebene Ansatz erlaubt die Nutzung existierender und neuer proprietärer Komponenten.

INTRODUÇÃO: A orientação dietética e o uso adequado de quelantes de fósforo são a base do tratamento da hiperfosfatemia. Assim, seu sucesso depende essencialmente da habilidade do paciente em entender e aderir ao plano dietético e ao uso dos quelantes. OBJETIVO: Avaliar a adesão e o conhecimento de pacientes hiperfosfatêmicos em hemodiálise sobre o tratamento da hiperfosfatemia. METODOLOGIA: Estudo transversal. Foram incluídos 112 pacientes em hemodiálise (60 homens; idade = 49,3 ± 13,3 anos), de cinco unidades de diálise, que apresentaram média de fósforo sérico > 5,5 mg/dL entre julho e dezembro de 2008 (média = 6,57 ± 0,73 mg/dL). Foi aplicado um questionário que incluía questões fechadas sobre consequências da hiperfosfatemia, alimentos ricos em fósforo, uso adequado dos quelantes e opinião do paciente sobre os motivos do insucesso do tratamento. Os parâmetros laboratoriais avaliados foram: fósforo, cálcio, paratormônio e ureia séricos, e a eficiência da diálise por meio do Kt/V. RESULTADOS : A média de acertos das questões do questionário foi de 78,5%. Com relação às razões do insucesso do tratamento da hiperfosfatemia, 87% dos pacientes assinalaram a resposta "porque eu como mais fósforo do que eu deveria" e/ ou "porque eu não tomo o quelante de fósforo como eu deveria". Entre os que afirmaram não utilizar o quelante corretamente, a maioria (62%) justificou o esquecimento como motivo. O fósforo sérico correlacionou-se diretamente com a ureia sérica (R = 0,33; p < 0,01) e inversamente com o Kt/V (R = -0,20; p < 0,05). Não houve correlação entre a fosfatemia, o nível de escolaridade e a pontuação no questionário. CONCLUSÃO: Os pacientes estudados apresentaram um bom nível de conhecimento sobre o tratamento da hiperfosfatemia, mas a maioria afirmou não ser aderente ao mesmo. Estratégias para melhorar a adesão ao tratamento são necessárias para diminuir a ocorrência da hiperfosfatemia nessa população.INTRODUCTION: Adequate dietary phosphorus intake and the use of phosphorus binders are the main tools for treating hyperphosphatemia. Thus, its success depends essentially on the patient's ability to understand and adhere to the dietary plan and the use of phosphate binders. OBJECTIVE: To evaluate hyperphosphatemic patients adherence and knowledge about phosphate control treatment. METHODS: This is a cross-sectional study. One hundred and twelve patients on hemodialysis (60 males; age = 49.3 ± 13.3 years), from five dialysis centers with mean serum phosphorus > 5.5 mg/dL between July and December of 2008 (mean = 6.57 ± 0.73 mg/dL) were included. A questionnaire with questions about the consequences of hyperphosphataemia, foods high in phosphorus, appropriate use of phosphate binders and patient's opinion about reasons for treatment failure was administered. Laboratory parameters assessed were serum urea, calcium, phosphorus and parathormony (PTH), and dialysis adequacy by means of urea Kt/V. RESULTS: The average score of questionnaire was 78.5%. Regarding the reasons for the failure of the treatment of hyperphosphataemia, 87% indicated the response "because I eat more phosphorus than I should" and / or "because I do not take the phosphate binder as I should". Among those who said they did not use phosphate binder correctly, most (62%) justified to forget as the reason. The serum phosphorus correlated directly with serum urea (R = 0.33, p < 0.01) and inversely with Kt/V (R = -0.20, p < 0.05). There was no correlation between the phosphorus, the education level and the scores on questionnaire. CONCLUSION: Patients showed a good level of knowledge about the hyperphosphatemia treatment, but the vast majority were noncompliant to that. Strategies to improve compliance are necessary to decrease the incidence of hyperphosphatemia in hemodialysis patients.

The place of Empedocles in metaphysical-mystical tradition This article argues that Empedocles was more than a pre-Socratic philosopher. His thinking was also essentially mystical and should be situated on a large map of metaphysical-mystical continuities with the following dimensions: A historically discernable cultural and religious pool, encompassing not only South-Eastern Europe, Asia Minor and Mediterranean Africa, but also the north-eastern Eurasian shamanic tradition, and India; an historically largely inaccesible esoteric tradition; a set of structural elements of the human psyche, running under and across historical religions through time; and the development of a new convergence of previously historically unconnected mystical traditions in the social and cultural circumstances of today. In particular, the article investigates similarities and differences between Empedocles and Indian (specifically Buddhist) views on various issues, such as the four roots and the cyclical dialectic of love and strife. In that context the article notes the remarkable interpretation of Empedocles by Peter Kingsley which seems to draw Empedocles closer to Buddhism, but without explicating this implication of his reception.

The objective of this study was to investigate the impact of a hunting trip on hunters‟ quality of life (QoL) as measured by life domains. A number of life domains that are directly related to a hunting trip may affect the QoL of hunters. A web-based survey was conducted and 158 responses were received. The statistical analysis consisted of descriptive statistics of the demographic profile of hunters, Cronbach‟s alpha, mean-inter-item-correlations, mean/standard deviation and a structural equation model. The results have indicated that social and travel life domains have a statistically significant effect on life domains overall, as well as on the QoL of hunters on a hunting trip as measured by life domains. The main contributions of the research are twofold: Firstly, it makes a contribution to the current literature on hunters and QoL; and secondly, it has identified the intangible aspects of hunting that may be used by owners of hunting products in marketing a hunting trip.

The main aim of this study is to report on the associations between age, critical skills and work perspectives (job satisfaction, career/organizational commitment and job characteristics) as perceived by resort employees. It highlights that age and critical skills play an important role towards work perspectives. A descriptive research design approach was followed. Three hundred and eighteen fully completed questionnaires were included in the statistical analysis, which included exploratory factor analysis, Spearman's rho and a structural equation model. Resort employees of different ages do not experience job characteristics differently. Older employees are often more experienced in the work environment, which contributes to an increase in job satisfaction, while younger employees who start building a career in the hospitality sector experience less job satisfaction. Older employees are more committed to their careers than younger employees. Critical skills have no influence on participants' perception of job characteristics. However, resort employees who have a variety of critical skills experience an increase in job satisfaction.

There is so far no available data about how the additive, synergistic, or antagonistic effects of the combined form of alpha-lactalbumin (α-La) and bacteriophages might modulate the cellular milieu of the host-pathogen interface. A co-culture of colonocytes and hepatocytes was stimulated with Pseudomonas aeruginosa PAO1 in the presence of KPP22 phage and incubated for 6 hours in medium alone or medium supplemented with bovine milk-origin α-La. The combination of KPP22 phage and α-La significantly inhibited P.a PAO1-elicited secretion of IL-1β, IL-6, and ICAM-1, which are the mediators and enzymes associated with the inflammatory response to an infectious-inflamed milieu. Cell viability was higher in the P.a PAO1+ KPP22 phage group compared to the P.a PAO1alone group. KPP22 phage and α-La, either alone or in combination, rescued P.a PAO1-induced aberrant PGE1/PGE2 production ratios. The convergence of ingested α-La and phages mitigates pro-inflammatory mediators. α-La leads to an increased sensitivity of opportunistic pathogenic bacteria to phages. Structural, functional, or immunological similarities between ingested α-La and phages play an important role in the mitigation of infection-driven pathobiological processes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Background: Gaps in optimal COPD management have been identified in clinical practice, with discrepancies between guideline recommendations and routine care. The reasons for such discrepancies are incompletely understood. The ELETHON survey aimed to identify physicians' attitudes towards general concepts of COPD management and, in particular, initiation of inhaled triple therapies., Method: ELETHON was a nationwide cross-sectional survey with general practitioners (GP) and pulmonary specialists (PS) working in the ambulatory outpatient setting in Germany, using a structured 17-item questionnaire (single or multiple choice questions) addressing the topics of secondary prevention, exacerbation detection, strategies for therapy escalation, choice of inhaled triple therapies and evaluation of treatment benefits., Results: Questionnaires filled by n=2028 GPs and n=371 PS were analyzed. In both groups, secondary prevention was deemed important in COPD care (GP/PS 76.4%/90.6%), with inhalation technique, vaccination status, and appropriate inhaled pharmacotherapy as key components. Activity/rehabilitation was rarely mentioned by GPs (48.3% vs. 84.5%). Exacerbations and symptomatic worsening were the main triggers for therapy escalation, but were not recorded in a structured way. "Hospitalization" and "≥2 ambulatory exacerbations" were mentioned most frequently as thresholds. Neither GPs nor PS measured eosinophils in the majority of patients. Fixed triple combinations were preferred, with availability of different treatment steps in the same device as important decision aid. Treatment success was evaluated by exacerbations, quality of life, symptoms, lung function and rescue medication use, while COPD Assessment Test (CAT) score was rarely used by GPs and PS., Discussion and Conclusion: The ELETHON survey identified gaps in COPD management in Germany. While secondary prevention is deemed important, escalation of inhaled therapy is undertaken rather late, the reported importance of vaccinations does not match current quota in German COPD patients, and non-pharmacological measures are often unused. Exacerbation and symptom documentation is rather subjective, validated questionnaires and blood eosinophils are of minor relevance. These results provide evidence of barriers and hidden potentials towards optimization of routine ambulatory care for COPD patients in Germany., Competing Interests: Referentenhonorare und ggf. Reisekosten (persönlich oder Institution): AstraZeneca, Berlin Chemie, Chiesi, Clario, GSK, Hormosan, MSD, Novartis, Orion Pharma, Pohl Boskamp, Forum Institut. Vergütung wissenschaftlicher Beratung (persönlich oder Institution): AstraZeneca, Berlin Chemie, Chiesi, Clario, GSK, MSD, Novartis, Orion Pharma, Pohl Boskamp, Sanofi, Sterna. Vergütung für die Durchführung klinischer Studien (Institution): AstraZeneca, Chiesi, GSK, Novartis, Pearl Therapeutics, Sterna GmbH., (The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).)

In vitro, spheroid models have become well established in cancer research because they can better mimic certain characteristics of in vivo tumours. However, interaction with the tumour microenvironment, such as cancer-associated fibroblasts, plays a key role in tumour progression. We initially focused on the interaction of tumour cells with fibroblasts. To model this interaction, we developed a spheroid model of ovarian cancer and fibroblasts. To this end, ovarian cancer cell lines and ex vivo primary cells were simultaneously and sequentially seeded with fibroblasts in a scaffold-free system at different ratios and subsequently characterized with respect to changes in morphology, proliferation, and viability. We demonstrated that co-cultures are able to form by far more compact spheroids, especially in cells that form aggregates in mono-culture. In addition, the co-cultures were able to increase proliferation and sensitivity to cisplatin. Simultaneous seeding led fibroblasts invade the core in both cell lines and primary cells. These results show differences in formation, firmness, and size between co-culture and mono-culture. Our model is designed to better represent and characterize the mutual influencing factors of fibroblasts and tumour cells. Fibroblast-supplemented multicellular spheroids are a valuable tool for tumour microenvironment interaction and new drug discovery., (© 2024. The Author(s).)

There is growing concern about the rising levels of dissolved organic matter (DOM) in surface waters across the Northern hemisphere. However, only limited research has been conducted to unveil its precise origin. Compositional changes along terrestrial-aquatic pathways can help determine the terrestrial sources of DOM in streams. Stream water, soil water and soil horizons were sampled at four sites representing typical settings within a forested catchment in the Ore Mountains (Erzgebirge, Germany) from winter 2020 to spring 2022. The samples were analyzed using pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). The resulting data were successfully subjected to semi-automatic processing of the molecular composition of DOM, reaching a percentage of identified peaks up to 98 %. Principal component analysis (PCA) and cluster analyses were carried out to identify distinct differences between DOM from the potential sources and in the streams. According to the PCA, organic soil horizons, soil water, and stream water samples could be clearly distinguished. Cluster analysis revealed that soil water DOM at all depths of Peats and deeper horizons of the Peaty Gleysols contributed the most to DOM in the stream section dominated by organic soils. In areas dominated by mineral soils, stream DOM resembled the DOM from the deeper mineral horizons of Cambisols and Podzols. Overall, our results suggested that most of the DOM exported from the catchment was derived from deeper mineral soil horizons, with little contribution of DOM derived from organic soils. Therefore, DOM fingerprint analysis of in-situ soil water proved to be a promising approach for tracing back the main sources of stream water DOM., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

In up to 18% of patients, recurrence is observed after curative radical cystectomy. Tumour recurrence occurs in most cases in the locoregional lymph node, as well as in the remaining urinary tract, but very rarely in the orthotopic neobladder. We report a case of multiple recurrence of urothelial carcinoma solely in the orthotopic neobladder of a male patient, 10 years after surgery, without involvement of the urethra or upper urinary tract. Therefore, the patient underwent resection of the neobladder with conversion to a sigmoid conduit. Nevertheless approximately 6 months later the patient developed widespread metastases and died a few months later. Currently, no guidelines are available for the treatment of these rare cases. We present our experience, together with revision of the current literature on this issue., Competing Interests: Die Autorinnen/Autoren geben an, dass kein Interessenkonflikt besteht., (Thieme. All rights reserved.)

The centrosome of the amoebozoan model Dictyostelium discoideum provides the best-established model for an acentriolar centrosome outside the Opisthokonta . Dictyostelium exhibits an unusual centrosome cycle, in which duplication is initiated only at the G2/M transition and occurs entirely during the M phase. Little is known about the role of conserved centrosomal kinases in this process. Therefore, we have generated knock-in strains for Aurora (AurK), CDK1, cyclin B, Nek2, and Plk, replacing the endogenous genes with constructs expressing the respective green fluorescent Neon fusion proteins, driven by the endogenous promoters, and studied their behavior in living cells. Our results show that CDK1 and cyclin B arrive at the centrosome first, already during G2, followed by Plk, Nek2, and AurK. Furthermore, CDK1/cyclin B and AurK were dynamically localized at kinetochores, and AurK in addition at nucleoli. The putative roles of all four kinases in centrosome duplication, mitosis, cytokinesis, and nucleolar dynamics are discussed.

We report on a 66-year-old neuro-urological female patient who, three years after implantation of a neurostimulator, experienced cecal necrosis due to strangulation caused by the cable of the device., Competing Interests: Die Autorinnen/Autoren geben an, dass kein Interessenkonflikt besteht., (Thieme. All rights reserved.)

Background: The choice between immunotherapy with a checkpoint inhibitor (CPI) and chemo-/immunotherapy (CIT) in patients with NSCLC stage IV is often discussed. There is some data that the effect of chemotherapy is influenced by TTF-1 expression. Little is known about the influence of thyreoid transcription factor 1 (TTF-1) expression on CIT and CPI therapy. We aimed to investigate the relationship between tumor TTF-1 expression and efficacy of CIT and CPI therapy., Patients and Methods: We retrospectively analysed 130 patients (age 68 ± 7 y) with NSCLC stage IV. Only patients with lung adenocarcinoma were included. Patients with ALK, ROS1, RET, MET, NTRK, EGFR, BRAF mutation were excluded. Patients were treated according to the guidelines with either CPI alone (pembrolizumab, nivolumab, atezolizumab, cemiplimab) or CIT (Carboplatin/Pemetrexed/Pembrolizumab, Carboplatin/Paclitaxel/Atezolizumab). We registered patients' characteristics including TTF-1 expression. Group 1 consisted of 40 patients with CPI and TTF-1 expression, group 2 were 26 patients with CPI and with no TTF-1 expression. Group 3 consisted of 41 patients with CIT and TTF-1 expression, group 4 were 23 patients with CIT and with no TTF-1 expression., Results: Group 1-4 showed comparable patients characteristics. Using cox-regression analysis, we found that TTF-1 expression resulted in an improved progression free survival (PFS) compared to patients with CPI and no TTF-1 expression (18 ± 3,15 vs. 5 ± 0,85 months, p = 0.004, 95% CI: 0,23 - 0,984). In patients, who were treated with CIT, PFS was also increased in patients with TTF-1 expression (9 ± 3,17 vs. 3 ± 0,399 months, p = 0.001, 95% CI: 0,23 - 0,85)., Conclusions: In a real-life setting, we found that TTF-1 expression is associated with an increased PFS. Patients with chemo-/immunotherapy and immunotherapy seem to have a better therapy response in pulmonary adenocarcinoma with TTF-1 expression., (© 2024. The Author(s).)

Peptide fibrillization is crucial in biological processes such as amyloid-related diseases and hormone storage, involving complex transitions between folded, unfolded, and aggregated states. We here employ light to induce reversible transitions between aggregated and nonaggregated states of a peptide, linked to the parathyroid hormone (PTH). The artificial light-switch 3-{[(4-aminomethyl)phenyl]diazenyl}benzoic acid (AMPB) is embedded into a segment of PTH, the peptide PTH 25-37 , to control aggregation, revealing position-dependent effects. Through in silico design, synthesis, and experimental validation of 11 novel PTH 25-37 -derived peptides, we predict and confirm the amyloid-forming capabilities of the AMPB-containing peptides. Quantum-chemical studies shed light on the photoswitching mechanism. Solid-state NMR studies suggest that β-strands are aligned parallel in fibrils of PTH 25-37 , while in one of the AMPB-containing peptides, β-strands are antiparallel. Simulations further highlight the significance of π-π interactions in the latter. This multifaceted approach enabled the identification of a peptide that can undergo repeated phototriggered transitions between fibrillated and defibrillated states, as demonstrated by different spectroscopic techniques. With this strategy, we unlock the potential to manipulate PTH to reversibly switch between active and inactive aggregated states, representing the first observation of a photostimulus-responsive hormone.