Search

Your search keyword '"Rousselle J."' showing total 93 results
Start Over Author "Rousselle J." Publication Type Academic Journals
93 results on '"Rousselle J."'

1. Detection of the Crab Nebula with the 9.7 m prototype Schwarzschild-Couder telescope

Author

Adams, C.B., Alfaro, R., Ambrosi, G., Ambrosio, M., Aramo, C., Arlen, T., Batista, P.I., Benbow, W., Bertucci, B., Bissaldi, E., Biteau, J., Bitossi, M., Boiano, A., Bonavolontà, C., Bose, R., Bouvier, A., Brill, A., Brown, A.M., Buckley, J.H., Byrum, K., Cameron, R.A., Canestrari, R., Capasso, M., Caprai, M., Covault, C.E., Depaoli, D., Errando, M., Fegan, S., Feng, Q., Fiandrini, E., Foote, Juniper, Fortin, P., Funk, S., Furniss, A., Garfias, F., Gent, A., Giglietto, N., Giordano, F., Giro, E., González, M.M., Guarino, V., Halliday, R., Hervet, O., Holder, J., Hughes, G., Humensky, T.B., Ionica, M., Iriarte, A., Jin, W., Johnson, C.A., Kaaret, P., Kieda, D., Kim, B., Kuznetsov, A., Lapington, J.S., Licciulli, F., Loporchio, S., Masone, V., Meagher, K., Meures, T., Mode, B.A.W., Mognet, S.A.I., Mukherjee, R., Nguyen, T., Nieto, D., Okumura, A., Otte, N., La Palombara, N., Pantaleo, F.R., Paoletti, R., Pareschi, G., Petrashyk, A., Di Pierro, F., Pueschel, E., Reynolds, P.T., Ribeiro, D., Richards, G., Roache, E., Ross, D., Rousselle, J., Rugliancich, A., Ruíz-Díaz-Soto, J., Santander, M., Schlenstedt, S., Schneider, M., Scuderi, S., Shang, R., Sironi, G., Stevenson, B., Stiaccini, L., Tajima, H., Taylor, L.P., Thornhill, J., Tosti, L., Tovmassian, G., Vagelli, V., Valentino, M., Vandenbroucke, J., Vassiliev, V.V., Di Venere, L., Wakely, S.P., Watson, J.J., White, R., Wilcox, P., Williams, D.A., Wood, M., Yu, P., and Zink, A.

Published
2021
Full Text
View/download PDF

2. Introducing the CTA concept

Author

Acharya, B.S., Actis, M., Aghajani, T., Agnetta, G., Aguilar, J., Aharonian, F., Ajello, M., Akhperjanian, A., Alcubierre, M., Aleksić, J., Alfaro, R., Aliu, E., Allafort, A.J., Allan, D., Allekotte, I., Amato, E., Anderson, J., Angüner, E.O., Antonelli, L.A., Antoranz, P., Aravantinos, A., Arlen, T., Armstrong, T., Arnaldi, H., Arrabito, L., Asano, K., Ashton, T., Asorey, H.G., Awane, Y., Baba, H., Babic, A., Baby, N., Bähr, J., Bais, A., Baixeras, C., Bajtlik, S., Balbo, M., Balis, D., Balkowski, C., Bamba, A., Bandiera, R., Barber, A., Barbier, C., Barceló, M., Barnacka, A., Barnstedt, J., Barres de Almeida, U., Barrio, J.A., Basili, A., Basso, S., Bastieri, D., Bauer, C., Baushev, A., Becerra, J., Becherini, Y., Bechtol, K.C., Becker Tjus, J., Beckmann, V., Bednarek, W., Behera, B., Belluso, M., Benbow, W., Berdugo, J., Berger, K., Bernard, F., Bernardino, T., Bernlöhr, K., Bhat, N., Bhattacharyya, S., Bigongiari, C., Biland, A., Billotta, S., Bird, T., Birsin, E., Bissaldi, E., Biteau, J., Bitossi, M., Blake, S., Blanch Bigas, O., Blasi, P., Bobkov, A., Boccone, V., Boettcher, M., Bogacz, L., Bogart, J., Bogdan, M., Boisson, C., Boix Gargallo, J., Bolmont, J., Bonanno, G., Bonardi, A., Bonev, T., Bonifacio, P., Bonnoli, G., Bordas, P., Borgland, A., Borkowski, J., Bose, R., Botner, O., Bottani, A., Bouchet, L., Bourgeat, M., Boutonnet, C., Bouvier, A., Brau-Nogué, S., Braun, I., Bretz, T., Briggs, M., Bringmann, T., Brook, P., Brun, P., Brunetti, L., Buanes, T., Buckley, J., Buehler, R., Bugaev, V., Bulgarelli, A., Bulik, T., Busetto, G., Buson, S., Byrum, K., Cailles, M., Cameron, R., Camprecios, J., Canestrari, R., Cantu, S., Capalbi, M., Caraveo, P., Carmona, E., Carosi, A., Carr, J., Carton, P.-H., Casanova, S., Casiraghi, M., Catalano, O., Cavazzani, S., Cazaux, S., Cerruti, M., Chabanne, E., Chadwick, P., Champion, C., Chen, A., Chiang, J., Chiappetti, L., Chikawa, M., Chitnis, V.R., Chollet, F., Chudoba, J., Cieślar, M., Cillis, A., Cohen-Tanugi, J., Colafrancesco, S., Colin, P., Colome, J., Colonges, S., Compin, M., Conconi, P., Conforti, V., Connaughton, V., Conrad, J., Contreras, J.L., Coppi, P., Corona, P., Corti, D., Cortina, J., Cossio, L., Costantini, H., Cotter, G., Courty, B., Couturier, S., Covino, S., Crimi, G., Criswell, S.J., Croston, J., Cusumano, G., Dafonseca, M., Dale, O., Daniel, M., Darling, J., Davids, I., Dazzi, F., De Angelis, A., De Caprio, V., De Frondat, F., de Gouveia Dal Pino, E.M., de la Calle, I., De La Vega, G.A., de los Reyes Lopez, R., De Lotto, B., De Luca, A., de Mello Neto, J.R.T., de Naurois, M., de Oliveira, Y., de Oña Wilhelmi, E., de Souza, V., Decerprit, G., Decock, G., Deil, C., Delagnes, E., Deleglise, G., Delgado, C., Della Volpe, D., Demange, P., Depaola, G., Dettlaff, A., Di Paola, A., Di Pierro, F., Díaz, C., Dick, J., Dickherber, R., Dickinson, H., Diez-Blanco, V., Digel, S., Dimitrov, D., Disset, G., Djannati-Ataï, A., Doert, M., Dohmke, M., Domainko, W., Dominis Prester, D., Donat, A., Dorner, D., Doro, M., Dournaux, J.-L., Drake, G., Dravins, D., Drury, L., Dubois, F., Dubois, R., Dubus, G., Dufour, C., Dumas, D., Dumm, J., Durand, D., Dyks, J., Dyrda, M., Ebr, J., Edy, E., Egberts, K., Eger, P., Einecke, S., Eleftheriadis, C., Elles, S., Emmanoulopoulos, D., Engelhaupt, D., Enomoto, R., Ernenwein, J.-P., Errando, M., Etchegoyen, A., Evans, P., Falcone, A., Fantinel, D., Farakos, K., Farnier, C., Fasola, G., Favill, B., Fede, E., Federici, S., Fegan, S., Feinstein, F., Ferenc, D., Ferrando, P., Fesquet, M., Fiasson, A., Fillin-Martino, E., Fink, D., Finley, C., Finley, J.P., Fiorini, M., Firpo Curcoll, R., Flores, H., Florin, D., Focke, W., Föhr, C., Fokitis, E., Font, L., Fontaine, G., Fornasa, M., Förster, A., Fortson, L., Fouque, N., Franckowiak, A., Fransson, C., Fraser, G., Frei, R., Albuquerque, I.F.M., Fresnillo, L., Fruck, C., Fujita, Y., Fukazawa, Y., Fukui, Y., Funk, S., Gäbele, W., Gabici, S., Gabriele, R., Gadola, A., Galante, N., Gall, D., Gallant, Y., Gámez-García, J., García, B., Garcia López, R., Gardiol, D., Garrido, D., Garrido, L., Gascon, D., Gaug, M., Gaweda, J., Gebremedhin, L., Geffroy, N., Gerard, L., Ghedina, A., Ghigo, M., Giannakaki, E., Gianotti, F., Giarrusso, S., Giavitto, G., Giebels, B., Gika, V., Giommi, P., Girard, N., Giro, E., Giuliani, A., Glanzman, T., Glicenstein, J.-F., Godinovic, N., Golev, V., Gomez Berisso, M., Gómez-Ortega, J., Gonzalez, M.M., González, A., González, F., González Muñoz, A., Gothe, K.S., Gougerot, M., Graciani, R., Grandi, P., Grañena, F., Granot, J., Grasseau, G., Gredig, R., Green, A., Greenshaw, T., Grégoire, T., Grimm, O., Grube, J., Grudzinska, M., Gruev, V., Grünewald, S., Grygorczuk, J., Guarino, V., Gunji, S., Gyuk, G., Hadasch, D., Hagiwara, R., Hahn, J., Hakansson, N., Hallgren, A., Hamer Heras, N., Hara, S., Hardcastle, M.J., Harris, J., Hassan, T., Hatanaka, K., Haubold, T., Haupt, A., Hayakawa, T., Hayashida, M., Heller, R., Henault, F., Henri, G., Hermann, G., Hermel, R., Herrero, A., Hidaka, N., Hinton, J., Hoffmann, D., Hofmann, W., Hofverberg, P., Holder, J., Horns, D., Horville, D., Houles, J., Hrabovsky, M., Hrupec, D., Huan, H., Huber, B., Huet, J.-M., Hughes, G., Humensky, T.B., Huovelin, J., Ibarra, A., Illa, J.M., Impiombato, D., Incorvaia, S., Inoue, S., Inoue, Y., Ioka, K., Ismailova, E., Jablonski, C., Jacholkowska, A., Jamrozy, M., Janiak, M., Jean, P., Jeanney, C., Jimenez, J.J., Jogler, T., Johnson, T., Journet, L., Juffroy, C., Jung, I., Kaaret, P., Kabuki, S., Kagaya, M., Kakuwa, J., Kalkuhl, C., Kankanyan, R., Karastergiou, A., Kärcher, K., Karczewski, M., Karkar, S., Kasperek, J., Kastana, D., Katagiri, H., Kataoka, J., Katarzyński, K., Katz, U., Kawanaka, N., Kellner-Leidel, B., Kelly, H., Kendziorra, E., Khélifi, B., Kieda, D.B., Kifune, T., Kihm, T., Kishimoto, T., Kitamoto, K., Kluźniak, W., Knapic, C., Knapp, J., Knödlseder, J., Köck, F., Kocot, J., Kodani, K., Köhne, J.-H., Kohri, K., Kokkotas, K., Kolitzus, D., Komin, N., Kominis, I., Konno, Y., Köppel, H., Korohoda, P., Kosack, K., Koss, G., Kossakowski, R., Kostka, P., Koul, R., Kowal, G., Koyama, S., Kozioł, J., Krähenbühl, T., Krause, J., Krawzcynski, H., Krennrich, F., Krepps, A., Kretzschmann, A., Krobot, R., Krueger, P., Kubo, H., Kudryavtsev, V.A., Kushida, J., Kuznetsov, A., La Barbera, A., La Palombara, N., La Parola, V., La Rosa, G., Lacombe, K., Lamanna, G., Lande, J., Languignon, D., Lapington, J., Laporte, P., Lavalley, C., Le Flour, T., Le Padellec, A., Lee, S.-H., Lee, W.H., Leigui de Oliveira, M.A., Lelas, D., Lenain, J.-P., Leopold, D.J., Lerch, T., Lessio, L., Lieunard, B., Lindfors, E., Liolios, A., Lipniacka, A., Lockart, H., Lohse, T., Lombardi, S., Lopatin, A., Lopez, M., López-Coto, R., López-Oramas, A., Lorca, A., Lorenz, E., Lubinski, P., Lucarelli, F., Lüdecke, H., Ludwin, J., Luque-Escamilla, P.L., Lustermann, W., Luz, O., Lyard, E., Maccarone, M.C., Maccarone, T.J., Madejski, G.M., Madhavan, A., Mahabir, M., Maier, G., Majumdar, P., Malaguti, G., Maltezos, S., Manalaysay, A., Mancilla, A., Mandat, D., Maneva, G., Mangano, A., Manigot, P., Mannheim, K., Manthos, I., Maragos, N., Marcowith, A., Mariotti, M., Marisaldi, M., Markoff, S., Marszałek, A., Martens, C., Martí, J., Martin, J.-M., Martin, P., Martínez, G., Martínez, F., Martínez, M., Masserot, A., Mastichiadis, A., Mathieu, A., Matsumoto, H., Mattana, F., Mattiazzo, S., Maurin, G., Maxfield, S., Maya, J., Mazin, D., Mc Comb, L., McCubbin, N., McHardy, I., McKay, R., Medina, C., Melioli, C., Melkumyan, D., Mereghetti, S., Mertsch, P., Meucci, M., Michałowski, J., Micolon, P., Mihailidis, A., Mineo, T., Minuti, M., Mirabal, N., Mirabel, F., Miranda, J.M., Mirzoyan, R., Mizuno, T., Moal, B., Moderski, R., Mognet, I., Molinari, E., Molinaro, M., Montaruli, T., Monteiro, I., Moore, P., Moralejo Olaizola, A., Mordalska, M., Morello, C., Mori, K., Mottez, F., Moudden, Y., Moulin, E., Mrusek, I., Mukherjee, R., Munar-Adrover, P., Muraishi, H., Murase, K., Murphy, A., Nagataki, S., Naito, T., Nakajima, D., Nakamori, T., Nakayama, K., Naumann, C., Naumann, D., Naumann-Godo, M., Nayman, P., Nedbal, D., Neise, D., Nellen, L., Neustroev, V., Neyroud, N., Nicastro, L., Nicolau-Kukliński, J., Niedźwiecki, A., Niemiec, J., Nieto, D., Nikolaidis, A., Nishijima, K., Nolan, S., Northrop, R., Nosek, D., Nowak, N., Nozato, A., O’Brien, P., Ohira, Y., Ohishi, M., Ohm, S., Ohoka, H., Okuda, T., Okumura, A., Olive, J.-F., Ong, R.A., Orito, R., Orr, M., Osborne, J., Ostrowski, M., Otero, L.A., Otte, N., Ovcharov, E., Oya, I., Ozieblo, A., Padilla, L., Paiano, S., Paillot, D., Paizis, A., Palanque, S., Palatka, M., Pallota, J., Panagiotidis, K., Panazol, J.-L., Paneque, D., Panter, M., Paoletti, R., Papayannis, A., Papyan, G., Paredes, J.M., Pareschi, G., Parks, G., Parraud, J.-M., Parsons, D., Paz Arribas, M., Pech, M., Pedaletti, G., Pelassa, V., Pelat, D., Perez, M.d.C., Persic, M., Petrucci, P.-O., Peyaud, B., Pichel, A., Pita, S., Pizzolato, F., Platos, Ł., Platzer, R., Pogosyan, L., Pohl, M., Pojmanski, G., Ponz, J.D., Potter, W., Poutanen, J., Prandini, E., Prast, J., Preece, R., Profeti, F., Prokoph, H., Prouza, M., Proyetti, M., Puerto-Gimenez, I., Pühlhofer, G., Puljak, I., Punch, M., Pyzioł, R., Quel, E.J., Quinn, J., Quirrenbach, A., Racero, E., Rajda, P.J., Ramon, P., Rando, R., Rannot, R.C., Rataj, M., Raue, M., Reardon, P., Reimann, O., Reimer, A., Reimer, O., Reitberger, K., Renaud, M., Renner, S., Reville, B., Rhode, W., Ribó, M., Ribordy, M., Richer, M.G., Rico, J., Ridky, J., Rieger, F., Ringegni, P., Ripken, J., Ristori, P.R., Riviére, A., Rivoire, S., Rob, L., Roeser, U., Rohlfs, R., Rojas, G., Romano, P., Romaszkan, W., Romero, G.E., Rosen, S., Rosier Lees, S., Ross, D., Rouaix, G., Rousselle, J., Rousselle, S., Rovero, A.C., Roy, F., Royer, S., Rudak, B., Rulten, C., Rupiński, M., Russo, F., Ryde, F., Sacco, B., Saemann, E.O., Saggion, A., Sahakian, V., Saito, K., Saito, T., Saito, Y., Sakaki, N., Sakonaka, R., Salini, A., Sanchez, F., Sanchez-Conde, M., Sandoval, A., Sandaker, H., Sant’Ambrogio, E., Santangelo, A., Santos, E.M., Sanuy, A., Sapozhnikov, L., Sarkar, S., Sartore, N., Sasaki, H., Satalecka, K., Sawada, M., Scalzotto, V., Scapin, V., Scarcioffolo, M., Schafer, J., Schanz, T., Schlenstedt, S., Schlickeiser, R., Schmidt, T., Schmoll, J., Schovanek, P., Schroedter, M., Schultz, C., Schultze, J., Schulz, A., Schure, K., Schwab, T., Schwanke, U., Schwarz, J., Schwarzburg, S., Schweizer, T., Schwemmer, S., Segreto, A., Seiradakis, J.-H., Sembroski, G.H., Seweryn, K., Sharma, M., Shayduk, M., Shellard, R.C., Shi, J., Shibata, T., Shibuya, A., Shum, E., Sidoli, L., Sidz, M., Sieiro, J., Sikora, M., Silk, J., Sillanpää, A., Singh, B.B., Sitarek, J., Skole, C., Smareglia, R., Smith, A., Smith, D., Smith, J., Smith, N., Sobczyńska, D., Sol, H., Sottile, G., Sowiński, M., Spanier, F., Spiga, D., Spyrou, S., Stamatescu, V., Stamerra, A., Starling, R., Stawarz, Ł., Steenkamp, R., Stegmann, C., Steiner, S., Stergioulas, N., Sternberger, R., Sterzel, M., Stinzing, F., Stodulski, M., Straumann, U., Strazzeri, E., Stringhetti, L., Suarez, A., Suchenek, M., Sugawara, R., Sulanke, K.-H., Sun, S., Supanitsky, A.D., Suric, T., Sutcliffe, P., Sykes, J., Szanecki, M., Szepieniec, T., Szostek, A., Tagliaferri, G., Tajima, H., Takahashi, H., Takahashi, K., Takalo, L., Takami, H., Talbot, G., Tammi, J., Tanaka, M., Tanaka, S., Tasan, J., Tavani, M., Tavernet, J.-P., Tejedor, L.A., Telezhinsky, I., Temnikov, P., Tenzer, C., Terada, Y., Terrier, R., Teshima, M., Testa, V., Tezier, D., Thuermann, D., Tibaldo, L., Tibolla, O., Tiengo, A., Tluczykont, M., Todero Peixoto, C.J., Tokanai, F., Tokarz, M., Toma, K., Torii, K., Tornikoski, M., Torres, D.F., Torres, M., Tosti, G., Totani, T., Toussenel, F., Tovmassian, G., Travnicek, P., Trifoglio, M., Troyano, I., Tsinganos, K., Ueno, H., Umehara, K., Upadhya, S.S., Usher, T., Uslenghi, M., Valdes-Galicia, J.F., Vallania, P., Vallejo, G., van Driel, W., van Eldik, C., Vandenbrouke, J., Vanderwalt, J., Vankov, H., Vasileiadis, G., Vassiliev, V., Veberic, D., Vegas, I., Vercellone, S., Vergani, S., Veyssiére, C., Vialle, J.P., Viana, A., Videla, M., Vincent, P., Vincent, S., Vink, J., Vlahakis, N., Vlahos, L., Vogler, P., Vollhardt, A., von Gunten, H.-P., Vorobiov, S., Vuerli, C., Waegebaert, V., Wagner, R., Wagner, R.G., Wagner, S., Wakely, S.P., Walter, R., Walther, T., Warda, K., Warwick, R., Wawer, P., Wawrzaszek, R., Webb, N., Wegner, P., Weinstein, A., Weitzel, Q., Welsing, R., Werner, M., Wetteskind, H., White, R., Wierzcholska, A., Wiesand, S., Wilkinson, M., Williams, D.A., Willingale, R., Winiarski, K., Wischnewski, R., Wiśniewski, Ł., Wood, M., Wörnlein, A., Xiong, Q., Yadav, K.K., Yamamoto, H., Yamamoto, T., Yamazaki, R., Yanagita, S., Yebras, J.M., Yelos, D., Yoshida, A., Yoshida, T., Yoshikoshi, T., Zabalza, V., Zacharias, M., Zajczyk, A., Zanin, R., Zdziarski, A., Zech, A., Zhao, A., Zhou, X., Ziętara, K., Ziolkowski, J., Ziółkowski, P., Zitelli, V., Zurbach, C., and Żychowski, P.

Published
2013
Full Text
View/download PDF

16. Hydrothermal method of preparation of actinide(IV) phosphate hydrogenphosphate hydrates and study of their conversion into actinide(IV) phosphate diphosphate solid solutions

Author

Dacheux, N., Grandjean, S., Rousselle, J., and Clavier, N.

Subjects
Actinide elements -- Chemical properties, Infrared spectroscopy -- Technology application, Phosphoric acid -- Chemical properties, X-rays -- Diffraction, X-rays -- Technology application, Technology application, Chemistry
Abstract

The stabilization of the tetravalent oxidation state of actinides in phosphate-based gels obtained from the mixture of acidic solutions containing cations and phosphoric acid is discussed. Results suggest the formation of crystallized compounds of actinide phosphate hydrogenphosphate hydrate due to the heat-treatment of gelatinous phases under hydrothermal conditions.

Published
2007

18. Analysis of the Seasonal Nature of Extreme Floods Across Canada.

Author

Rémillard, L., Rousselle, J., Ashkar, F., and Sparks, D.

Subjects
FLOODS, NATURAL disasters, HYDRAULIC measurements, HYDROMETER, FLUID mechanics
Abstract

The purpose of this paper is to study the seasonal characteristics of floods in Canada. Its principal objective is to judge the relevance of seasonal analysis and to attempt to present a unified set of methodologies for handling the problems of seasonally varied flows. Another objective is to broadly identify various regions of Canada for which seasonal analysis would be appropriate. Partial duration series and the exceedance model are used because they are well suited to this type of analysis. The focus will be on improved estimation of low-frequency/large-magnitude flood events. Two methods are investigated as a means for grouping flood series on a seasonal basis in a meaningful way: One method is based on flood generating phenomena, and the other divides the year into “homogeneous” periods, which could be called “seasons.” Seasonal analyses were performed on 166 hydrometric stations in all regions of Canada. Synthesis of the results has made it possible to broadly identify regions for which an analysis of seasonal flood variations may be the most relevant. In general, these are the southern regions of the country. Specifically, there is an indication from the results obtained for the Maritime provinces, southern Ontario and the Prairie provinces, that these regions are well suited to seasonal analysis. Also, the results show that frequency analysis for stations recording one or more rare events, or having flood subpopulations that are clearly distinct, may be significantly improved by using a seasonal approach. [ABSTRACT FROM AUTHOR]

Published
2004
Full Text
View/download PDF

19. Performance of Neural Networks in Daily Streamflow Forecasting.

Author

Birikundavyi, S., Labib, R., Trung, H. T., and Rousselle, J.

Subjects
STREAMFLOW, ARTIFICIAL neural networks
Abstract

Feed-forward multilayer neural networks are widely used as predictors in several fields of applications. The purpose of this study is to investigate the performance of neural networks as potential models capable of forecasting daily streamflows. Once an appropriate network has been identified, a comparison approach is used to evaluate it against a conceptual model presently in use by the Alcan Company. The Mistassibi River, located in northeastern Quebec, serves as the case study, and results based on mean square errors and Nash coefficients show that artificial neural networks outperform the deterministic model PREVIS for up to 5-day-ahead forecasts. Moreover, the results obtained with the neural network are also superior to the ones obtained with a classic autoregressive model coupled with a Kalman filter. [ABSTRACT FROM AUTHOR]

Published
2002
Full Text
View/download PDF

21. Improving Forecasts of Nile Flood Using SST Inputs in TFN Model.

Author

Awadallah, A. G. and Rousselle, J.

Subjects
WATER supply, STREAMFLOW, EL Nino, PARAMETER estimation
Abstract

Egypt depends on the Nile River for all of its water resources. Using the streamflows' history, the large fluctuations of the Nile flood cause the best predictions to be unsatisfactory. The purpose of this paper is to stochastically forecast the Nile summer runoff one-season-ahead using, as inputs, an El Nin˜o-southern oscillation (ENSO) sea surface temperatures (SSTs) signal in the East Pacific and SSTs in the South Indian Ocean. Causality between inputs and outputs is established, and a multiple-input transfer function with noise (TFN) model is built for forecasting purposes. The model explains 63% of the variability of the Nile flood with relatively stable parameters. The mean of absolute percentage error of forecasts is 6% calculated on a data set that was not used in the parameter estimation. The model is parsimonious, and its behavior agrees with the most recent studies in climatology. The forecasting ability of the model is high for extreme floods and severe drought years, except when the South Atlantic Ocean displays a strong warm signal opposite to the El Nin˜o-southern oscillation cold signal. [ABSTRACT FROM AUTHOR]

Published
2000
Full Text
View/download PDF

22. Hybrid Stochastic Model for Daily Flows Simulation in Semiarid Climates.

Author

Evora, N. D. and Rousselle, J.

Subjects
STOCHASTIC processes, RIVERS, ARID regions, FLOODS
Abstract

An innovative stochastic model for the purpose of daily flows simulations is presented in this paper. This model is particularly well suited for the generation of daily river flows in semiarid climates. The previous models have not preserved basic features of daily flow hydrographs, such as peaks and recessions, and have failed to reproduce the long low water and short flood periods due to the temporal distribution of precipitation in semiarid climates such as Sahelian regions. This hybrid stochastic model uses a product model that simulates an intermittent impulse series. The impulse magnitude is the flow increment from one day to the next. The intermittent impulse series enables the definition over time of alternate rising and falling limbs. On the rising limbs, the generated impulse magnitudes help to produce the daily flows. However, on the falling limbs, the flows are produced by a deterministic model using a time-varying recession coefficient that is, for the first time, introduced in stochastic modeling of daily flows. Application of this model for daily flows simulation at the Bakel Station on the Senegal River (West Africa) has provided good results as shown by the analysis of the synthetic daily flow series. [ABSTRACT FROM AUTHOR]

Published
2000
Full Text
View/download PDF

24. Use of Partial Duration Series for Single-Station and Regional Analysis of Floods.

Author

Birikundavyi, S. and Rousselle, J.

Subjects
FLOODS, POISSON distribution, HYDROGRAPHY
Abstract

Techniques for flood estimation at a single station and in a regional context using partial duration series are analyzed. This involves using a Poisson distribution to describe the occurrence of floods, while the generalized Pareto distribution is used for flood magnitudes. The fitting technique used is that of probability-weighted moments, and the regionalization scheme adopted is the index flood method. The problem of threshold choice is also analyzed. Examples given throughout the paper use partial-duration series abstracted from daily hydrographs recorded at hydrometric stations in the province of Ontario, Canada. [ABSTRACT FROM AUTHOR]

Published
1997
Full Text
View/download PDF

27. Some Problems of Flood Analysis.

Author

Todorovic, P. and Rousselle, J.

Abstract

This paper is a continuation of a previous one in which a stochastic model for analyzing excessive streamflows was presented. The model was based on the assumption that flood peak exceedances are independent, identically distributed random variables and that their occurrence is subject to the Poisson law. In the present paper a model is developed for nonidentically distributed exceedances on the assumption that only those exceedances during a particular season may be considered identically distributed. From this hypothesis the distribution function of the maximum flood peak exceedance in an arbitrary interval of time (0, t] is determined. The results are then applied to the 72-year record of the Greenbrier River at Alderson, West Virginia. The theoretical and observed results agree reasonably well. [ABSTRACT FROM AUTHOR]

Published
1971
Full Text
View/download PDF

38. New markers in Anopheles gambiae salivary glands after Plasmodium berghei infection.

Author

Zocevic A, Carmi-Leroy A, Sautereau J, d'Alayer J, Lenormand P, Rousselle JC, Namane A, and Choumet V

Subjects
Animals, Anopheles metabolism, Biomarkers, Chromatography, High Pressure Liquid, Electrophoresis, Gel, Two-Dimensional, Female, Host-Parasite Interactions, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Anopheles microbiology, Plasmodium berghei physiology, Salivary Glands metabolism
Abstract

In malaria, mosquito saliva and salivary glands play central roles in the multi-faceted interactions that occur among the parasite, its vector, and its host. Analyzing the processes involved in the survival and maintenance of the Plasmodium parasite in mosquito organs, and in its transmission into vertebrate hosts, may lead to the identification of new molecular targets for parasite control. We used comparative two-dimensional gel polyacrylamide electrophoresis (2D-PAGE), surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS), and high-performance liquid chromatography (HPLC), followed by Edman sequencing, to study saliva and salivary gland samples from Anopheles gambiae mosquitoes infected or not with Plasmodium berghei. Quantitative 2D-PAGE profile analysis showed that the intensities of seven spots were affected by the presence of the parasite in the salivary glands. Most of the proteins identified possessed a signal peptide. SELDI-TOF-MS revealed 32 proteins/peptides whose peak intensities differed between the Plasmodium-infected and non-infected control groups. Quantitative comparison of HPLC profiles of low-molecular-weight components from salivary gland extracts revealed several peptides and proteins with levels that were modulated by parasite infection. The results of these complementary approaches suggest that the infection of female A. gambiae mosquitoes by P. berghei alters the production levels of several salivary gland proteins and peptides, some of which (e.g., protein cE5, B3VDI9_ANOGA, and AGAP008216-PA) are known or predicted to be secreted in saliva and involved in blood feeding.

Published
2013
Full Text
View/download PDF

39. Mast cell-dependent B and T lymphocyte activation is mediated by the secretion of immunologically active exosomes.

Author

Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, David B, Namane A, and Mécheri S

Subjects
Animals, Antigens analysis, Antigens metabolism, Cell Fractionation, Cell Line, Cell-Free System immunology, Cells, Cultured, Cytokines biosynthesis, Cytokines physiology, Cytoplasmic Vesicles ultrastructure, Electrophoresis, Polyacrylamide Gel, Mast Cells ultrastructure, Mice, Mice, Inbred A, Mice, Inbred C57BL, Mice, Inbred DBA, Mice, Knockout, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spleen cytology, Spleen immunology, Spleen metabolism, Tumor Cells, Cultured, Ultracentrifugation, B-Lymphocytes immunology, Cytoplasmic Vesicles immunology, Cytoplasmic Vesicles metabolism, Lymphocyte Activation immunology, Mast Cells immunology, Mast Cells metabolism, T-Lymphocytes immunology
Abstract

Mitogenic activity of bone marrow-derived mouse mast cells and mast cell lines P815 and MC/9 on B and T lymphocytes is present in their culture supernatants. To identify this activity, mast cells were incubated in serum-free medium and the supernatant was subjected to differential centrifugation, which resulted in two fractions, the hypodense and dense fraction (pellet). When analyzed for their mitogenic activity on spleen cells, all activity was found to be associated with the dense fraction. Electron microscopy studies revealed the presence in this fraction of small vesicles called exosomes with a heterogeneous size from 60 to 100 nm of diameter. When cocultured with spleen cells, purified exosomes induced blast formation, proliferation, as well as IL-2 and IFN-gamma production, but no detectable IL-4. Similar data were obtained by injecting exosomes into naive mice. In contrast to mast cell lines, a pretreatment with IL-4 is required for bone marrow-derived mast cells to secrete active exosomes. Structurally, exosomes were found to harbor immunologically relevant molecules such as MHC class II, CD86, LFA-1, and ICAM-1. These findings indicate that mast cells can represent a critical component of the immunoregulatory network through secreted exosomes that display mitogenic activity on B and T lymphocytes both in vitro and in vivo.

Published
2001
Full Text
View/download PDF

40. Nonspecific B and T cell-stimulatory activity mediated by mast cells is associated with exosomes.

Author

Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, Namane A, David B, and Mécheri S

Subjects
Animals, Antigens, CD analysis, B7-2 Antigen, Cells, Cultured, Cytokines biosynthesis, Histocompatibility Antigens Class II analysis, Intercellular Adhesion Molecule-1 analysis, Lymphocyte Function-Associated Antigen-1 analysis, Membrane Glycoproteins analysis, Mice, Mice, Inbred DBA, Secretory Vesicles chemistry, B-Lymphocytes immunology, Lymphocyte Activation, Mast Cells immunology, Secretory Vesicles physiology, T-Lymphocytes immunology
Abstract

Bone marrow-derived mouse mast cells (BMMC) and mast cell lines P815 and MC9 have recently been shown to induce antigen-independent B and T lymphocyte activation. It has been demonstrated that a physical contact between mast cells and B and T lymphocytes is not necessary since mast cell supernatants contain full activity. Electron microscopy studies revealed the presence in mast cell supernatants of small vesicles called exosomes with a heterogeneous size from 60 to 100 nm of diameter. When cocultured with spleen cells, purified exosomes induce B and T cell blast formation, proliferation as well as IL-2 and IFN-gamma production. In contrast to P815 and MC9 mast cell lines, a pretreatment with IL-4 is required for BMMC to produce active exosomes. Structurally, these exosomes were found to harbor immunologically relevant molecules such as MHC class II, CD86, LFA-1 and ICAM-1. Here we provide for the first time the evidence that mast cells use exosomes as sophisticated messengers to communicate with cells of the immune system., (Copyright 2001 S. Karger AG, Basel)

Published
2001
Full Text
View/download PDF

41. Structural requirements of 5-hydroxytryptamine-moduline analogues to interact with the 5-hydroxytryptamine1B receptor.

Author

Plantefol M, Rousselle JC, Massot O, Bernardi E, Schoofs AR, Pourrias B, Ollivier R, and Fillion G

Subjects
Animals, Binding Sites, Binding, Competitive, Chemical Phenomena, Chemistry, Physical, Male, Neuropeptides metabolism, Oligopeptides metabolism, Protein Binding, Protein Conformation, Rats, Rats, Wistar, Receptor, Serotonin, 5-HT1B, Receptors, Serotonin chemistry, Receptors, Serotonin drug effects, Structure-Activity Relationship, Neuropeptides chemistry, Oligopeptides chemistry, Receptors, Serotonin metabolism
Abstract

5-Hydroxytryptamine-moduline is an endogenous cerebral tetrapeptide that regulates the activity of 5-hydroxytryptamine1B receptors. Direct binding of 5-[3H]hydroxytryptamine-moduline on rat brain homogenate evidenced the existence of two interacting sites for the peptide, very likely corresponding to different conformations of the 5-hydroxytryptamine1B receptor: The peptide first binds to a low-affinity state of the receptor (pIC50 = 7.68+/-0.14) and then induces (or stabilizes) a high-affinity complex (pIC50 = 11.62+/-0.18). This work focuses on the ability of 5-hydroxytryptamine-moduline analogues to recognize the high- and low-affinity sites for 5-hydroxytryptamine-moduline. The results obtained show that the two conformers of the 5-hydroxytryptamine1B receptor have similar but not identical binding pockets for 5-hydroxytryptamine-moduline. These two sites proved to be stereoselective and selective for tetrapeptides and favored the binding of peptides with hydrophobic amino acids in positions 1 and 4, serine in position 2, and a short amino acid in position 3. However, the serine in position 2 seems to be more important for the interaction of the peptide with the low-affinity site than the high-affinity one, which only needs a short hydrophobic amino acid in position 2.

Published
1999
Full Text
View/download PDF

42. 5-HT1B receptors: a novel target for lithium. Possible involvement in mood disorders.

Author

Massot O, Rousselle JC, Fillion MP, Januel D, Plantefol M, and Fillion G

Subjects
3T3 Cells, Animals, Antidepressive Agents pharmacology, Antidepressive Agents therapeutic use, Binding, Competitive, Blood Platelets metabolism, CHO Cells, Cations metabolism, Cricetinae, Dose-Response Relationship, Drug, Guanosine 5'-O-(3-Thiotriphosphate) metabolism, Guinea Pigs, Humans, Lithium therapeutic use, Mice, Mood Disorders drug therapy, Psychomotor Performance drug effects, Rats, Receptor, Serotonin, 5-HT1B, Receptors, Serotonin drug effects, Serotonin metabolism, Serotonin Antagonists pharmacology, Serotonin Antagonists therapeutic use, Sulfur Radioisotopes, Synaptosomes drug effects, Synaptosomes metabolism, Lithium pharmacology, Mood Disorders metabolism, Receptors, Serotonin metabolism
Abstract

Lithium ion is widely used to treat depressive patients, often as an initial helper for antidepressant drugs or as a mood stabilizer; however, the toxicity of the drug raises serious problems, because the toxic doses of lithium are quite close to the therapeutic ones. Thus, precise characterization of the target(s) involved in the therapeutic activity of lithium is of importance. The present work, carried out at molecular, cellular, and in vivo levels, demonstrates that 5-HT1B receptor constitutes a molecular target for lithium. Several reasons suggest that this interaction is more likely related to the therapeutic properties of lithium than to its undesirable effects. First, the observed biochemical and functional interaction occurs at concentrations that precisely correspond to effective therapeutic doses of lithium. Second, 5-HT1B receptors are well characterized as controlling the activity of the serotonergic system, which is known to be involved in affective disorders and the mechanism of action of various antidepressants. These findings represent progress in our knowledge of the mechanism of action of lithium that may facilitate clinical use of the ion and also open new directions in the research of antidepressant therapies.

Published
1999
Full Text
View/download PDF

43. Endoproteolytic activity in mammalian brain membranes cleaves 5-hydroxytryptamine-moduline into dipeptides.

Author

Plantefol M, Rousselle JC, Bernardi E, Schoofs AR, Pourrias B, and Fillion G

Subjects
Animals, Binding Sites, In Vitro Techniques, Leucine analogs & derivatives, Leucine pharmacology, Ligands, Male, Membranes, Neuropeptides chemical synthesis, Neuropeptides chemistry, Neuropeptides pharmacology, Oligopeptides chemical synthesis, Oligopeptides chemistry, Oligopeptides pharmacology, Protease Inhibitors chemical synthesis, Protease Inhibitors chemistry, Protease Inhibitors metabolism, Protease Inhibitors pharmacology, Rats, Rats, Wistar, Structure-Activity Relationship, Brain metabolism, Dipeptides metabolism, Endopeptidases metabolism, Neuropeptides metabolism, Oligopeptides metabolism
Abstract

This work was intended to determine which enzymatic activities from crude synaptosomal mammalian brain membranes could qualify for the status of 5-hydroxytryptamine-moduline (5-HT-moduline, LSAL, Leu-Ser-Ala-Leu) inactivating enzymes. An enzymatic assay for 5-HT-moduline metabolism was developed using [3H]5-HT-moduline measurement and high performance liquid chromatography (HPLC) technique to identify and quantify 5-HT-moduline metabolites. 5-HT-moduline metabolism displayed all characteristics of metalloprotease activity: sensitivity to divalent ion chelators, reactivation by Zn2+ ions and a pH optimum in the 7-8 range. Bestatin, an aminopeptidase inhibitor, allowed the identification of two enzymatic activities responsible for this metabolism: a bestatin-sensitive aminopeptidase and an endoprotease cleaving 5-HT-moduline into LS (Leu-Ser) and AL (Ala-Leu) dipeptides. This latter enzyme was shown to have a Km of 37.1 +/- 3.6 microM and a Vmax of 5.5 micromol min(-1) l(-1) per mg of protein. Moreover, this enzyme was insensitive to peptidyl dipeptidase A (angiotensin converting enzyme, EC 3.4.15.1), endothelin converting enzyme and neutral endopeptidase (neprylisin, EC 3.4.24.11) inhibitors and displayed some specificity among 5-HT-moduline-analogues and in particular recognized only tetrapeptides. These results, together with the isolation of the LS and AL metabolites [Rousselle, J.C., Massot, O., Delepierre, M., Zifa, E., Rousseau, B., Fillion, G., 1996. Isolation and characterization of an endogenous peptide from rat brain interacting specifically with the serotonergic 1B receptor subtypes. J. Biol. Chem. 271, 726-735] during the purification process of 5-HT-moduline are strong arguments for the physiological implication of this endoprotease in 5-HT-moduline metabolism.

Published
1999
Full Text
View/download PDF

44. Functional properties of 5-HT-moduline in the immune system: a model for central nervous system investigation.

Author

Grimaldi B, Sibella-Arguelles C, Bonnin A, Fillion MP, Massot O, Rousselle JC, Seznec JC, and Fillion G

Subjects
Animals, Antibody Formation, Brain immunology, Cell Line, Cerebral Ventricles drug effects, Cerebral Ventricles physiology, Cerebral Ventricles physiopathology, Gene Expression Regulation immunology, Gene Expression Regulation physiology, Humans, Injections, Intraventricular, Mice, Rats, Receptor, Serotonin, 5-HT1B, Stress, Psychological immunology, Brain physiopathology, Lymphocytes physiology, Neuropeptides immunology, Neuropeptides pharmacology, Oligopeptides immunology, Oligopeptides pharmacology, Receptors, Serotonin physiology
Published
1998
Full Text
View/download PDF

45. 5-HT-moduline, a 5-HT(1B/1D) receptor endogenous modulator, interacts with dopamine release measured in vivo by microdialysis.

Author

Bentué-Ferrer D, Reymann JM, Rousselle JC, Massot O, Bourin M, Allain H, and Fillion G

Subjects
Animals, Brain metabolism, Drug Interactions, Male, Microdialysis, Pyridines pharmacology, Pyrroles pharmacology, Rats, Rats, Sprague-Dawley, Receptor, Serotonin, 5-HT1B, Receptor, Serotonin, 5-HT1D, Receptors, Serotonin drug effects, Serotonin Receptor Agonists pharmacology, Brain drug effects, Dopamine metabolism, Neuropeptides pharmacology, Oligopeptides pharmacology
Abstract

5-Hydroxytryptamine-moduline (5-HT-moduline) is an endogenous tetrapeptide (Leu-Ser-Ala-Leu) recently isolated and characterized from mammalian brain. This compound interacts with 5-HT1B receptors as a non-competitive, high-affinity antagonist and has the properties of an allosteric modulator. 5-HT-moduline could play an important role in the regulation of serotonergic transmission and also, through heteroreceptors, dopaminergic transmission. The aim of this work was to examine the potential ability of 5-HT-moduline to modify the basal extracellular concentration of dopamine and its metabolites (3-methoxytyramine, dihydroxyphenylacetic acid and homovanillic acid), in the rat striatum and to determine its potential interaction with the stimulating activity of a specific 5-HT1B receptor agonist, 3-(1,2,5,6-tetrahydropyrid-4-yl) pyrrolo [3,2-b] pyrid-5-one (CP-93,129), on the release of dopamine. The technique is based on in vivo microdialysis using probes implanted in the striatum of the conscious rat. Results showed that the perfusion of 5-HT-moduline directly into this structure (1.25 mM) increased the striatal level of dopamine by two-fold (104% of the absolute basal release values, P = 0.0015) and that of 3-methoxytyramine by 3-fold (293%, P = 0.0001) without any change in the terminal metabolite concentrations. The intrastriatal administration of CP-93,129 induced a statistically significant, dose-dependent increase of dopamine levels (P < 0.0001). Coperfusion of 5-HT-moduline did not significantly alter the effect of CP-93,129 at 0.1 and 0.5 mM, but appeared to have an additive effect on the lowest dose (P = 0.0406). The results obtained show that 5-HT-moduline directly administered into the striatum increases the release of dopamine in this area. Presumably, this effect results from the desensitization of 5-HT1B receptors located on dopamine terminals. However, the fact that a 5-HT1B receptor agonist (CP-93,129) also increased the release of dopamine in the striatum and that 5-HT-moduline exhibited a slight additive effect with that of a low concentration of CP-93,129 suggests that the two substances interact with different mechanisms.

Published
1998
Full Text
View/download PDF

46. Borreliacidal antibody production against outer surface protein C of Borrelia burgdorferi.

Author

Rousselle JC, Callister SM, Schell RF, Lovrich SD, Jobe DA, Marks JA, and Wieneke CA

Subjects
Animals, Antibodies, Bacterial classification, Antigens, Bacterial genetics, Antigens, Bacterial immunology, Bacterial Outer Membrane Proteins genetics, Female, Humans, Lyme Disease blood, Mice, Mice, Inbred C3H, Temperature, Antibodies, Bacterial immunology, Bacterial Outer Membrane Proteins immunology, Borrelia burgdorferi Group immunology, Lyme Disease immunology
Abstract

Early Lyme borreliosis sera with significant titers of anti-outer surface protein C (OspC) borreliacidal antibodies were identified. Human anti-OspC borreliacidal antibodies could be either IgM or IgG. Significant concentrations of borreliacidal activity were detected after vaccination of mice with OspC. Detection of anti-OspC borreliacidal activity was dependent on surface expression of OspC by Borrelia burgdorferi isolate 50772. The ability of OspC to induce borreliacidal antibodies in vivo and after vaccination offers another possible explanation for the ability of vaccination with OspC to protect against infection with B. burgdorferi. Furthermore, detection of anti-OspC borreliacidal antibodies, especially IgM antibodies, in early Lyme borreliosis sera provides additional evidence that borreliacidal antibody detection may be useful for the serodiagnosis of early Lyme borreliosis.

Published
1998
Full Text
View/download PDF

47. Specific interaction of 5-HT-moduline with human 5-HT1b as well as 5-HT1d receptors expressed in transfected cultured cells.

Author

Rousselle JC, Plantefol M, Fillion MP, Massot O, Pauwels PJ, and Fillion G

Subjects
Allosteric Regulation drug effects, Animals, Binding Sites drug effects, Cells, Cultured, Dose-Response Relationship, Drug, Free Radical Scavengers metabolism, GTP-Binding Proteins metabolism, Humans, Neuroglia drug effects, Neuroglia metabolism, Rats, Receptor, Serotonin, 5-HT1B, Receptor, Serotonin, 5-HT1D, Receptors, Serotonin metabolism, Serotonin metabolism, Transfection drug effects, Neuropeptides pharmacology, Oligopeptides pharmacology, Receptors, Serotonin drug effects
Abstract

5-HT1B receptors are the predominant auto- and heteroreceptors located on serotonergic and non-serotonergic terminals where they regulate the neuronal release of neurotransmitters. 5-HT-moduline (Leu-Ser-Ala-Leu) has been shown to specifically interact with a very high apparent affinity and in a non-competitive manner with 5-HT1B receptors (Massot et al. 1996; Rousselle et al. 1996). Using transfected cells expressing either 5-HT1B or 5-HT1D receptors, it was shown that 5-HT-moduline prevents the binding of [3H]5-HT to 5-HT1B as well as to 5-HT1D receptors with similar biochemical characteristics: the IC50 of the peptide was 1.2x10(-12) M for 5-HT1B and 9x10(-13) M for 5-HT1D receptors. The observed effect corresponds to a marked decrease of the maximal binding for [3H]5-HT on 5-HT1B (-51.2 +/- 1%) as well as 5-HT1D binding (-47.2 +/- 7.7% of the control binding) whereas the affinity of 5-HT is increased by a factor close to 3. No effect is observed using the "scrambled" peptide (Ala-Leu-Leu-Ser). Parallel assays using transfected cells expressing 5-HT1A or 5-ht6 receptors did not show any significant change induced by the peptide under similar assay conditions. The interaction of the peptide was also studied on the functional activity related to the stimulation of the receptors as measured by the increase in [35S]GTPgammaS binding reflecting the coupling of the receptor to the G-protein. 5-HT-moduline yields an antagonistic effect on the 5-HT induced coupling with a corresponding IC50 = 1.2 +/- 0.7x10(-12) M for 5-HT1B and 9.8 +/- 4.0x10(-12) M for 5-HT1D receptors, respectively. The present results demonstrate that 5-HT-moduline interacts with 5-HT1D as well as 5-HT1B receptors and possesses a non-competitive antagonistic activity, likely corresponding to its role of endogenous allosteric modulator, specific for both 5-HT1B and 5-HT1D receptors.

Published
1998
Full Text
View/download PDF

48. Mapping of 5-HT-moduline binding sites in guinea-pig brain by film and digital autoradiography.

Author

Cloëz-Tayarani I, Cardona A, Sarhan H, Rousselle JC, Massot O, Edelman L, and Fillion G

Subjects
Animals, Autoradiography methods, Binding Sites physiology, Guinea Pigs, Image Processing, Computer-Assisted, In Vitro Techniques, Tissue Distribution, Brain metabolism, Neuropeptides metabolism, Oligopeptides metabolism
Abstract

5-HT-moduline is a cerebral tetrapeptide [Leu-Ser-Ala-Leu] that was recently isolated from bovine brain tissue and shown to interact specifically with 5-HT1B receptors, particularly in rodents. The pharmacological properties of 5-HT1B receptors in rodents are different from those in other species. In order to better understand the role of this peptide in non-rodent species, we determined the distribution of 5-HT-moduline binding sites in guinea-pig brain using both the film autoradiography and digital autoradiography with a newly developed high resolution beta-imaging techniques. We found that 5-HT-moduline binding sites were expressed in various brain regions. Quantitative analysis showed that densities of binding sites were similar to those observed previously in rat brain. Regions with the highest labelling included cortex, septum, hippocampus and some regions of basal ganglia. Our results extend previous data and show that 5-HT-moduline interacts with the two forms of 5-HT1B receptors that are distinct pharmacologically. By this interaction, 5-HT-moduline may play an important role in regulating the functional activity of 5-HT1B receptors, thereby contributes to the pathophysiology of serotonergic transmission., (Copyright 1998 Elsevier Science B.V. All rights reserved.)

Published
1998
Full Text
View/download PDF

49. Autoradiographic characterization of [3H]-5-HT-moduline binding sites in rodent brain and their relationship to 5-HT1B receptors.

Author

Cloëz-Tayarani I, Cardona A, Rousselle JC, Massot O, Edelman L, and Fillion G

Subjects
Animals, Autoradiography, Binding Sites, Cattle, Male, Mice, Rats, Rats, Wistar, Receptors, Serotonin analysis, Tritium, Brain metabolism, Neuropeptides metabolism, Oligopeptides metabolism, Receptors, Serotonin metabolism
Abstract

5-HT-moduline is an endogenous tetrapeptide [Leu-Ser-Ala-Leu (LSAL)] that was first isolated from bovine brain tissue. To understand the physiological role of this tetrapeptide, we studied the localization of 5-HT-moduline binding sites in rat and mouse brains. Quantitative data obtained with a gaseous detector of beta-particles (beta-imager) indicated that [3H]-5-HT-moduline bound specifically to rat brain sections with high affinity (Kd = 0.77 nM and Bmax = 0. 26 dpm/mm2). Using film autoradiography in parallel, we found that 5-HT-moduline binding sites were expressed in a variety of rat and mouse brain structures. In 5-HT1B receptor knock-out mice, the specific binding of [3H]-5-HT-moduline was not different from background labeling, indicating that 5-HT-moduline targets are exclusively located on the 5-HT1B receptors. Although the distribution of 5-HT-moduline binding sites was similar to that of 5-HT1B receptors, they did not overlap totally. Differences in distribution patterns were found in regions containing either high levels of 5-HT1B receptors such as globus pallidus and subiculum that were poorly labeled or in other regions such as dentate gyrus of hippocampus and cortex where the relative density of 5-HT-moduline binding sites was higher than that of 5-HT1B receptors. In conclusion, our data, based on autoradiographic localization, indicate that 5-HT-moduline targets are located on 5-HT1B receptors present both on 5-HT afferents and postsynaptic neurons. By interacting specifically with 5-HT1B receptors, this tetrapeptide may play a pivotal role in pathological states such as stress that involves the dysfunction of 5-HT neurotransmission.

Published
1997
Full Text
View/download PDF

50. Immunocytochemical localization of neurons expressing 5-HT-moduline in the mouse brain.

Author

Grimaldi B, Fillion MP, Bonnin A, Rousselle JC, Massot O, and Fillion G

Subjects
Animals, Antibodies immunology, Brain anatomy & histology, Immunohistochemistry, Mice, Neuropeptides immunology, Oligopeptides immunology, Brain metabolism, Neurons metabolism, Neuropeptides metabolism, Oligopeptides metabolism
Abstract

The localization of 5-HT-moduline, an endogenous cerebral tetrapeptide (LSAL) which specifically interacts with 5-HT1B receptors (Rousselle et al., 1996; Massot et al., 1996) was examined in mouse brain using an immunocytochemistry technique with a polyclonal anti-peptide antibody highly specific for this tetrapeptide. Highest levels of 5-HT-moduline immunoreactivity were observed in the cerebral cortex including cingulate, retrosplenial, parietal and pyriform cortical areas and in the basal ganglia. Intense immunoreactivity also occurred in the hippocampus, subiculum, various hypothalamic and thalamic nuclei and in some midbrain regions such as the substantia nigra and the superior colliculi. Immunoreactive neurons generally showed intense and extensive labelling of the perikarya and dendritic arborizations with moderate to heavy characteristic deposits of reaction product along plasma membranes and within cytoplasm while the nuclei were devoid of reaction product. The results obtained indicated that 5-HT-moduline immunoreactivity was heterogenously distributed in neuronal structures of mouse brain. The distribution of 5-HT-moduline immunoreactivity closely correlated with that of 5-HT-moduline specific binding sites as visualized by autoradiography (Massot et al., 1996). Moreover, it seems to overlap with the distribution of serotonergic innervation and also with that of 5-HT1B receptors in mouse brain (Boschert et al., 1994; Bruinvels et al., 1994; Chopin et al., 1994; Langlois et al., 1995). These data provide evidence that 5-HT-moduline immunoreactivity is located in cells with the morphological appearance of neurones. Its localization in brain areas which also contain 5-HT1B receptors, is in good agreement with previous demonstrations that this peptide specifically interacts with 5-HT1B receptors to regulate their functional activity and accordingly controls the modulatory activity of the serotoninergic system on various CNS functions.

Published
1997
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results