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To date, there has been a lag between the rise in E-cigarette use and an understanding of the long-term health effects. Inhalation of E-cigarette aerosol delivers high doses of nicotine, raises systemic cytokine levels, and compromises cardiopulmonary function. The consequences for muscle function have not been thoroughly investigated. The present study tests the hypothesis that exposure to nicotine-containing aerosol impairs locomotor muscle function, limits exercise tolerance, and interferes with muscle repair in male mice. Nicotine-containing aerosol reduced the maximal force produced by the extensor digitorum longus (EDL) by 30%-40% and, the speed achieved in treadmill running by 8%. Nicotine aerosol exposure also decreased adrenal and increased plasma epinephrine and norepinephrine levels, and these changes in catecholamines manifested as increased muscle and liver glycogen stores. In nicotine aerosol exposed mice, muscle regenerating from overuse injury only recovered force to 80% of noninjured levels. However, the structure of neuromuscular junctions (NMJs) was not affected by e-cigarette aerosols. Interestingly, the vehicle used to dissolve nicotine in these vaping devices, polyethylene glycol (PG) and vegetable glycerin (VG), decreased running speed by 11% and prevented full recovery from a lengthening contraction protocol (LCP) injury. In both types of aerosol exposures, cardiac left ventricular systolic function was preserved, but left ventricular myocardial relaxation was altered. These data suggest that E-cigarette use may have a negative impact on muscle force and regeneration due to compromised glucose metabolism and contractile function in male mice.NEW & NOTEWORTHY In male mice, nicotine-containing E-cigarette aerosol compromises muscle contractile function, regeneration from injury, and whole body running speeds. The vehicle used to deliver nicotine, propylene glycol, and vegetable glycerin, also reduces running speed and impairs the restoration of muscle function in injured muscle. However, the predominant effects of nicotine in this inhaled aerosol are evident in altered catecholamine levels, increased glycogen content, decreased running capacity, and impaired recovery of force following an overuse injury.

This study compared school-aged monolingual and bilingual English-as-a-foreign-language (EFL) learners in terms of understanding metaphors on recall, multiple-choice, and reasoning tasks. It also examined the relationship between cognitive capacity and understanding metaphors on different measures. A hundred and thirty Persian–Turkish early bilinguals and 122 monolingual Persian-speaking EFL learners took three different tests of metaphor comprehension and the Figural Intersections Test, a test of cognitive capacity. Bilinguals outperformed monolinguals in terms of cognitive capacity and understanding metaphors on two of the tasks, though with a small effect size. Furthermore, there was a significant positive relationship between cognitive capacity and the scores on the multiple-choice and reasoning tests, but not the recall test. Results suggest that bilingual L3 learners have an edge in understanding metaphors, reflecting a cognitive advantage. [ABSTRACT FROM AUTHOR]

Background: Some menopausal symptoms can negatively affect people's daily activities. The work ability index (WAI) investigates employees at risk of work-related disabilities. The current study, conducted at Gonabad University of Medical Sciences, evaluated the association between the severity of symptoms during the menopausal transition and the work ability index. Materials & Methods: In this descriptive study, 170 female subjects in the age range of menopause transition (42 to 54 years) were selected from the list of female employees at Gonabad University of Medical Sciences, using the simple random sampling method. Data were collected using a socio-demographic characteristics form, the work ability questionnaire, and the severity of menopause symptoms questionnaire. Finally, the data were analyzed in SPSS software (version 22). ANOVA and Pearson correlation coefficient were applied for data analysis. Results: The mean±SD of the WAI score was 38.88±6.55. The mean ± SD of intensity of physical, mental, and urogenital menopausal symptoms were 7.44±2.96, 6.46±2.67, and 5.32±2.30, respectively. The Pearson correlation coefficient between menopausal symptom severity and work ability was significantly negative (p<0.001, r=-0.418). Conclusion: The reduced work ability of female employees may cause increased absenteeism, additional costs for the employer, reduced employee productivity, and increased production costs, especially in the industry and service sectors. [ABSTRACT FROM AUTHOR]

We are presenting a 35‐year‐old woman with past medical history of disseminated leiomyomatosis who presented with heart failure symptoms and was found to have post‐capillary pulmonary hypertension and high cardiac output state in right heart catheterization secondary to a huge pelvic arterio‐venous fistula. [ABSTRACT FROM AUTHOR]

INTRODUCTION. Coronavirus disease 2019 (COVID-19) mainly involves the respiratory system but can also affect the digestive system and cause several gastrointestinal manifestations. Acute pancreatitis has been reported as one of the rare presentations of COVID-19. This study aimed to systematically review case reports on COVID-19-associated acute pancreatitis. METHODS. Publications were retrieved through a comprehensive search in four databases on October 1, 2021. Eligible ones that demonstrated the potential association of acute pancreatitis and COVID-19 were included for data extraction. RESULTS. After screening 855 citations, 82 articles containing 95 cases were included, and their data were extracted. The most common presentation was abdominal pain (88/95, 92.6%), followed by nausea/vomiting (61/95, 64.2%). Mortality was reported in 10.5% of cases. The initial presentation was acute pancreatitis, COVID-19, and concomitant in 32.6% (31/95), 48.4% (46/95), and 18.9% (18/95) of cases, respectively. Among the included cases, acute pancreatitis severity was associated with ICU admission, COVID-19 severity, and the outcome. Also, the initial presentation was associated with COVID-19 severity (P values =0.05). CONCLUSIONS. Current evidence indicates that acute pancreatitis can present before, after, or concomitant with COVID-19. Appropriate investigations should be performed in cases with suspicious clinical presentations. Longitudinal studies should address whether or not, there is a causative relationship between COVID-19 and acute pancreatitis. [ABSTRACT FROM AUTHOR]

An efficient and rapid fabrication procedure for ZIF-8 crystals through ultrasonic assisted strategy (30min) was reported. Additionally, the crystallinity, morphology, chemical bonding, and porosity of ZIF-8 were fully characterized by XRD, and FT-IR. The Response surface methodology approach was established to optimize a series of adsorption conditions: initial concentration (10-400 mg/L), dose (0.02-1 g/L), and time (20-60 min) for Red 141 and Violet-5r removals. Particularly, plausible adsorption mechanisms (electrostatic interactions and π--π stacking) were elucidated, and isotherm models were rigorously studied by three-parameter equations (Langmuir, Freundlich, Tempkin). The adsorption isotherm data showed the adsorption of reactive dyes by ZIF-8, was consistent with the Langmuir isotherm model. The kinetics parameters were in accord with the pseudo-second-order equation, which implied that the adsorption rate was mainly controlled by the chemisorption mechanism. Through advantageous effectiveness involving good reusability (4 recycles), and maximum adsorption capacities (250-200 mg/g), it is recommendable to utilize ZIF-8 as a good adsorbent for the dyes remediation. [ABSTRACT FROM AUTHOR]

Coronavirus 2019 (COVID‐19) is a global concern for public health. Thus, early and accurate diagnosis is a critical step in management of this infectious disease. Currently, RT‐PCR is routine diagnosis test for COVID‐19, but it has some limitations and false negative results. enzyme‐linked immunosorbent assay (ELISA) against SARS‐CoV‐2 antigens seems to be an appropriate approach for serodiagnosis of COVID‐19. In the current study, an ELISA system, using a recombinant nucleocapsid (N) protein, was developed for the detection of IgM and IgG antibodies to SARS‐CoV‐2. The related protein was expressed, purified, and used in an ELISA system. Sera samples (67) for COVID‐19 patients, as well as sera samples from healthy volunteers (112), along with sera samples from non‐COVID‐19 patients were examined by the ELISA system. The expression and purity of the recombinant N protein were approved by SDS‐PAGE and Western blotting. The sensitivity of ELISA system was 91.04 and 92.53% for the detection of IgG and IgM antibodies, respectively. Moreover, the specificity of the developed ELISA system for IgG and IgM were 98.21 and 97.32%, respectively. Our developed ELISA system showed satisfactory sensitivity and specificity for the detection of antiSARS‐CoV‐2 IgM and IgG antibodies and could be used as a complementary approach for proper diagnosis of COVID‐19. [ABSTRACT FROM AUTHOR]

Escherichia coli (E. coli) is shocked by various temperature processes in milk, which forces the organism to make proteins as a result of changes in the synthesis of enzymes that might give the strain special characteristics. The purpose of this study was to investigate the effects of the heat shock factor on changing the results of biochemical and molecular tests among E. coli strains obtained from ice cream and non-pasteurized milk when compared to a reference strain from the American-type culture collection (ATCC) in order to determine the phenotypic variation caused by the temperature conditions of the manufacturing process. Furthermore, isolates with characteristics similar to E. coli were discovered, but they were not E. coli and caused some ambiguity. To test the E. coli contamination of traditional and industrial ice cream, 82 samples were chosen at random. SDS-PAGE and 16S rDNA sequencing were carried out, as well as phenotypic testing. Isolated strains did not exactly match the reference strain. The results of biochemical testing and protein analysis revealed that the isolates were diverse. Samples E. coli phenons were classified. In the electrophoresis, the ice cream strain had two protein bands in the 20.75 and 23.59 kDa ranges that were distinct from the reference strain. These isolates appear to experience alterations in enzyme characteristics and structural proteins as a result of being exposed to various temperature conditions, such as pasteurization and frigidity. When compared to the reference strain, the calculated similarity percentage of the elicited isolate varied from 60 to 70%. The electrophoretic patterns of E. coli isolated elicited from milk samples differed from E. coli isolated obtained from the ice cream. The distinctions were in the intensity or position of the bands. The results also revealed that when isolates are subjected to thermal stresses, they exhibit a pattern similar to that of ice cream isolates. These considerations are made because a change in protein composition might result in a change in biochemical features, resulting in uncertainty in its identification. Sequences revealed that the sequences were related to E. coli 16S rDNA, despite differences in phenotypic and electrophoretic features between the isolated bacteria and the reference strain E. coli ATCC 25922. Our findings revealed that 16S rDNA could potentially be used to instantly implement an appropriate preventive measure for the purpose of identifying this type of bacteria and avoid some ambiguity. [ABSTRACT FROM AUTHOR]

Background: Cardiovascular diseases, with an estimated 18.6 million deaths per year, are the leading cause of death worldwide. One of the major risk factors is elevated blood low-density lipoprotein cholesterol (LDL-C) secondary to multiple environmental and genetic factors. Genes involved in LDL-C metabolism are the targets of the most common treatment options. Advanced molecular techniques could pave the way for identifying novel targets in dyslipidemia therapies. The LIM domain and actin-binding 1 (LIMA1) gene binds to the NPC1L1 protein and facilitates its more efficient recycling to the plasma membrane. Inhibition of LIMA1 could disrupt cellular cholesterol hemostasis with a probable decrease in blood LDL-C levels. Objectives: The present study was designed to knock out exon 2 of the LIMA1 gene using lentiviruses as an in vitro model for reducing cholesterol absorption. Methods: A CRISPR/Cas9 system with dual guide RNAs (gRNAs) was designed to completely excise exon 2 of LIMA1. Two gRNAs (gRNA1 and gRNA2) were cloned in the LentiCRISPR v2 vector. LentiCRISPR viruses were produced in the HEK293T cell line to encode the CRISPR/Cas9 complex structure. HepG2 cell lines were transduced with two different LentiCRISPR viruses simultaneously. Results: Exon 2 deletion was detected by PCR, gel electrophoresis, and subsequent Sanger sequencing of the PCR product. Exon 2 deletion caused a frameshift mutation, and the subsequent production of nonfunctional transcripts led to gene knockout. The dual gRNA CRISPR/Cas9 system could be used in gene editing setups. Conclusions: The in vitro knockout model of LIMA1 could be considered as preliminary work to study the role and mechanism of action of the LIMA1 protein, along with its potential as a target for hypercholesterolemia therapy. [ABSTRACT FROM AUTHOR]

Introduction: Preparedness a social behavior with public trust the prerequisite for proper social functioning can reduce disaster sufferings. The aim of this study was to determine the public trust, preparedness, and the influencing factors regarding the COVID-19 pandemic situation in Iran. Methods: This online cross-sectional study conducted on Tehran residents (≥18 years) during the fifth epidemic wave. The tool designed to assess the intention to prepare, public trust, and the subscales. T-test used to compare the means and linear regression to determine the factors influencing on the outcomes. Results: About 26.5% of 407 participants (mean age standard deviation: 40.8 (12.8) years) showed low trust. The mean levels of total trust (P = 0.011), general trust (P = 0.048), and trust to managing authorities (P = 0.018) were significantly lower in men. Adjusted to confounding variables, total trust was lower in men (ß = −3.8, P = 0.01) and less educated (ß = −6.48, P = 0.02) but higher in high-income (ß = 5.7, P = 0.02) people. Only 20% of people were highly prepared. Intention to prepare was higher in families having the elderly (ß = 5.72.8, P = 0.048). Conclusion: Low trust in the managing authorities and their provided information tend to less considering health and preparedness measures in society. [ABSTRACT FROM AUTHOR]