14 results on '"Ramaswamy, Raghavendran"'
Search Results
2. Parasitic nematode fatty acid- and retinol-binding proteins compromise host immunity by interfering with host lipid signaling pathways.
- Author
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Parks, Sophia C, Nguyen, Susan, Nasrolahi, Shyon, Bhat, Chaitra, Juncaj, Damian, Lu, Dihong, Ramaswamy, Raghavendran, Dhillon, Harpal, Fujiwara, Hideji, Buchman, Anna, Akbari, Omar S, Yamanaka, Naoki, Boulanger, Martin J, and Dillman, Adler R
- Subjects
Animals ,Animals ,Genetically Modified ,Drosophila melanogaster ,Fatty Acid-Binding Proteins ,Helminth Proteins ,Host-Parasite Interactions ,Nematoda ,Nematode Infections ,Retinol-Binding Proteins ,Infectious Diseases ,Prevention ,Inflammatory and immune system ,Infection ,Virology ,Microbiology ,Immunology ,Medical Microbiology - Abstract
Parasitic nematodes cause significant morbidity and mortality globally. Excretory/secretory products (ESPs) such as fatty acid- and retinol- binding proteins (FARs) are hypothesized to suppress host immunity during nematode infection, yet little is known about their interactions with host tissues. Leveraging the insect parasitic nematode, Steinernema carpocapsae, we describe here the first in vivo study demonstrating that FARs modulate animal immunity, causing an increase in susceptibility to bacterial co-infection. Moreover, we show that FARs dampen key components of the fly immune response including the phenoloxidase cascade and antimicrobial peptide (AMP) production. Our data also reveal that FARs deplete lipid signaling precursors in vivo as well as bind to these fatty acids in vitro, suggesting that FARs elicit their immunomodulatory effects by altering the availability of lipid signaling molecules necessary for an efficient immune response. Collectively, these data support a complex role for FARs in immunosuppression in animals and provide detailed mechanistic insight into parasitism in phylum Nematoda.
- Published
- 2021
3. A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites.
- Author
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Suarez, Catherine, Lentini, Gaëlle, Ramaswamy, Raghavendran, Maynadier, Marjorie, Aquilini, Eleonora, Berry-Sterkers, Laurence, Cipriano, Michael, Chen, Allan, Bradley, Peter, Striepen, Boris, Boulanger, Martin, and Lebrun, Maryse
- Subjects
Animals ,Carrier Proteins ,Cell Line ,Exocytosis ,Fibroblasts ,Host-Parasite Interactions ,Humans ,Microscopy ,Electron ,Transmission ,Microscopy ,Fluorescence ,Organelles ,Parasites ,Phospholipids ,Plasmodium falciparum ,Protozoan Proteins ,Toxoplasma - Abstract
Members of the Apicomplexa phylum, including Plasmodium and Toxoplasma, have two types of secretory organelles (micronemes and rhoptries) whose sequential release is essential for invasion and the intracellular lifestyle of these eukaryotes. During invasion, rhoptries inject an array of invasion and virulence factors into the cytoplasm of the host cell, but the molecular mechanism mediating rhoptry exocytosis is unknown. Here we identify a set of parasite specific proteins, termed rhoptry apical surface proteins (RASP) that cap the extremity of the rhoptry. Depletion of RASP2 results in loss of rhoptry secretion and completely blocks parasite invasion and therefore parasite proliferation in both Toxoplasma and Plasmodium. Recombinant RASP2 binds charged lipids and likely contributes to assembling the machinery that docks/primes the rhoptry to the plasma membrane prior to fusion. This study provides important mechanistic insight into a parasite specific exocytic pathway, essential for the establishment of infection.
- Published
- 2019
4. Structural and mechanistic insights into the function of the unconventional class XIV myosin MyoA from Toxoplasma gondii
- Author
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Powell, Cameron J., Ramaswamy, Raghavendran, Kelsen, Anne, Hamelin, David J., Warshaw, David M., Bosch, Jürgen, Burke, John E., Ward, Gary E., and Boulanger, Martin J.
- Published
- 2018
5. Proteomic Profiling Reveals the Transglutaminase-2 Externalization Pathway in Kidneys after Unilateral Ureteric Obstruction
- Author
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Furini, Giulia, Schroeder, Nina, Huang, Linghong, Boocock, David, Scarpellini, Alessandra, Coveney, Clare, Tonoli, Elisa, Ramaswamy, Raghavendran, Ball, Graham, Verderio, Claudia, Johnson, Timothy S., and Verderio, Elisabetta A.M.
- Published
- 2018
- Full Text
- View/download PDF
6. The structure of Plasmodium falciparum 3D7_0606800 reveals a bi‐lobed architecture that supports re‐annotation as a Venus Flytrap protein
- Author
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Parker, Michelle L., Ramaswamy, Raghavendran, van Gordon, Kyle, Powell, Cameron J., Bosch, Jürgen, and Boulanger, Martin J.
- Published
- 2017
- Full Text
- View/download PDF
7. The Trypanosoma brucei MISP family of invariant proteins is co-expressed with BARP as triple helical bundle structures on the surface of salivary gland forms, but is dispensable for parasite development within the tsetse vector.
- Author
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Casas-Sanchez, Aitor, Ramaswamy, Raghavendran, Perally, Samïrah, Haines, Lee R., Rose, Clair, Aguilera-Flores, Marcela, Portillo, Susana, Verbeelen, Margot, Hussain, Shahid, Smithson, Laura, Yunta, Cristina, Lehane, Michael J., Vaughan, Sue, van den Abbeele, Jan, Almeida, Igor C., Boulanger, Martin J., and Acosta-Serrano, Álvaro
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TRYPANOSOMA , *SALIVARY glands , *HELICAL structure , *TRYPANOSOMA brucei , *SURFACE structure , *MEMBRANE glycoproteins - Abstract
Trypanosoma brucei spp. develop into mammalian-infectious metacyclic trypomastigotes inside tsetse salivary glands. Besides acquiring a variant surface glycoprotein (VSG) coat, little is known about the metacyclic expression of invariant surface antigens. Proteomic analyses of saliva from T. brucei-infected flies identified, in addition to VSG and Brucei Alanine-Rich Protein (BARP) peptides, a family of GPI-anchored surface proteins herein named as Metacyclic Invariant Surface Proteins (MISP) because of its predominant expression on the surface of metacyclic trypomastigotes. The MISP family is encoded by five paralog genes with >80% protein identity, which are exclusively expressed by salivary gland stages of the parasite and peak in metacyclic stage, as shown by confocal microscopy and immuno-high resolution scanning electron microscopy. Crystallographic analysis of a MISP isoform (MISP360) and a high confidence model of BARP revealed a triple helical bundle architecture commonly found in other trypanosome surface proteins. Molecular modelling combined with live fluorescent microscopy suggests that MISP N-termini are potentially extended above the metacyclic VSG coat, and thus could be tested as a transmission-blocking vaccine target. However, vaccination with recombinant MISP360 isoform did not protect mice against a T. brucei infectious tsetse bite. Lastly, both CRISPR-Cas9-driven knock out and RNAi knock down of all MISP paralogues suggest they are not essential for parasite development in the tsetse vector. We suggest MISP may be relevant during trypanosome transmission or establishment in the vertebrate's skin. Author summary: The Trypanosoma brucei group of parasites are exclusively transmitted to the vertebrate host by the tsetse vector alongside insect saliva. To better understand trypanosome transmission, we investigated the protein composition of T. brucei-infected tsetse saliva using a mass spectrometry proteomics approach. We found that, in addition to proteins from tsetse saliva and Sodalis glossinidius (a bacterial tsetse symbiont), trypanosome-infected saliva contains several parasite surface glycoproteins, including a partially characterized family of invariant proteins herein named Metacyclic Invariant Surface Proteins (MISP). We show that MISP is primarily expressed, together with mVSG and BARP, on the surface of the infectious metacyclic stage of T. brucei. Its triple helix bundle architecture appears tethered to the outer membrane by an extended GPI-anchored C-terminal tail that putatively projects MISP above the VSG coat. Our findings provide new insights into the surface architecture of the T. brucei metacyclic stage and describes the challenges associated with developing transmission-blocking vaccines against tsetse-transmitted trypanosomes. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
8. Identification and Functional Characterization of Peptides With Antimicrobial Activity From the Syphilis Spirochete, Treponema pallidum.
- Author
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Houston, Simon, Schovanek, Ethan, Conway, Kate M. E., Mustafa, Sarah, Gomez, Alloysius, Ramaswamy, Raghavendran, Haimour, Ayman, Boulanger, Martin J., Reynolds, Lisa A., and Cameron, Caroline E.
- Subjects
ANTIMICROBIAL peptides ,SPIROCHETES ,SYPHILIS ,TREPONEMA pallidum ,PEPTIDE antibiotics ,ANTI-infective agents ,GRAM-negative bacteria ,GRAM-positive bacteria - Abstract
The etiological agent of syphilis, Treponema pallidum ssp. pallidum , is a highly invasive "stealth" pathogen that can evade the host immune response and persist within the host for decades. This obligate human pathogen is adept at establishing infection and surviving at sites within the host that have a multitude of competing microbes, sometimes including pathogens. One survival strategy employed by bacteria found at polymicrobial sites is elimination of competing microorganisms by production of antimicrobial peptides (AMPs). Antimicrobial peptides are low molecular weight proteins (miniproteins) that function directly via inhibition and killing of microbes and/or indirectly via modulation of the host immune response, which can facilitate immune evasion. In the current study, we used bioinformatics to show that approximately 7% of the T. pallidum proteome is comprised of miniproteins of 150 amino acids or less with unknown functions. To investigate the possibility that AMP production is an unrecognized defense strategy used by T. pallidum during infection, we developed a bioinformatics pipeline to analyze the complement of T. pallidum miniproteins of unknown function for the identification of potential AMPs. This analysis identified 45 T. pallidum AMP candidates; of these, Tp0451a and Tp0749 were subjected to further bioinformatic analyses to identify AMP critical core regions (AMPCCRs). Four potential AMPCCRs from the two predicted AMPs were identified and peptides corresponding to these AMPCCRs were experimentally confirmed to exhibit bacteriostatic and bactericidal activity against a panel of biologically relevant Gram-positive and Gram-negative bacteria. Immunomodulation assays performed under inflammatory conditions demonstrated that one of the AMPCCRs was also capable of differentially regulating expression of two pro-inflammatory chemokines [monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8)]. These findings demonstrate proof-of-concept for our developed AMP identification pipeline and are consistent with the novel concept that T. pallidum expresses AMPs to defend against competing microbes and modulate the host immune response. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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9. Structural characterization of Treponema pallidum Tp0225 reveals an unexpected leucine‐rich repeat architecture.
- Author
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Ramaswamy, Raghavendran, Houston, Simon, Loveless, Bianca, Cameron, Caroline E., and Boulanger, Martin J.
- Subjects
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TREPONEMA pallidum , *SPIROCHETES , *PATHOGENIC bacteria , *LEUCINE , *GLOBUS pallidus , *PROTEIN-protein interactions - Abstract
The phylogenetically divergent spirochete bacterium Treponema pallidum subsp. pallidum is the causative agent of syphilis. Central to the capacity of T. pallidum to establish infection is the ability of the pathogen to attach to a diversity of host cells. Many pathogenic bacteria employ leucine‐rich repeat (LRR) domain‐containing proteins to mediate protein–protein interactions, including attachment to host components and establishment of infection. Intriguingly, T. pallidum expresses only one putative LRR domain‐containing protein (Tp0225) with an unknown function. In an effort to ascribe a function to Tp0225, a comprehensive phylogenetic analysis was first performed; this investigation revealed that Tp0225 clusters with the pathogenic clade of treponemes. Its crystal structure was then determined to 2.0 Å resolution using Pt SAD phasing, which revealed a noncanonical architecture containing a hexameric LRR core with a discontinuous β‐sheet bridged by solvent molecules. Furthermore, a surface‐exposed, hydrophobic pocket, which was found in Tp0225 but is largely absent in canonical LRR domains from other pathogenic bacteria, may serve to coordinate a hydrophobic ligand. Overall, this study provides the first structural characterization of the sole LRR domain‐containing protein from T. pallidum and offers insight into the unique molecular landscape of this important human pathogen. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
10. A Toxoplasma gondii locus required for the direct manipulation of host mitochondria has maintained multiple ancestral functions.
- Author
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Blank, Matthew L., Parker, Michelle L., Ramaswamy, Raghavendran, Powell, Cameron J., English, Elizabeth D., Adomako‐Ankomah, Yaw, Pernas, Lena F., Workman, Sean D., Boothroyd, John C., Boulanger, Martin J., and Boyle, Jon P.
- Subjects
TOXOPLASMA gondii ,MITOCHONDRIA ,STRUCTURAL analysis (Science) ,ADENOSINE diphosphate ribose ,HOST-bacteria relationships - Abstract
Summary: The Toxoplasma gondii locus mitochondrial association factor 1 (MAF1) encodes multiple paralogs, some of which mediate host mitochondrial association (HMA). Previous work showed that HMA was a trait that arose in T. gondii through neofunctionalization of an ancestral MAF1 ortholog. Structural analysis of HMA‐competent and incompetent MAF1 paralogs (MAF1b and MAF1a, respectively) revealed that both paralogs harbor an ADP ribose binding macro‐domain, with comparatively low (micromolar) affinity for ADP ribose. Replacing the 16 C‐terminal residues of MAF1b with those of MAF1a abrogated HMA, and we also show that only three residues in the C‐terminal helix are required for MAF1‐mediated HMA. Importantly these same three residues are also required for the in vivo growth advantage conferred by MAF1b, providing a definitive link between in vivo proliferation and manipulation of host mitochondria. Co‐immunoprecipitation assays reveal that the ability to interact with the mitochondrial MICOS complex is shared by HMA‐competent and incompetent MAF1 paralogs and mutants. The weak ADPr coordination and ability to interact with the MICOS complex shared between divergent paralogs may represent modular ancestral functions for this tandemly expanded and diversified T. gondii locus. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
11. Structural characterization reveals a novel bilobed architecture for the ectodomains of insect stage expressed Trypanosoma brucei PSSA-2 and Trypanosoma congolense ISA.
- Author
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Ramaswamy, Raghavendran, Goomeshi Nobary, Sarah, Eyford, Brett A., Pearson, Terry W., and Boulanger, Martin J.
- Abstract
African trypanosomiasis, caused by parasites of the genus Trypanosoma, is a complex of devastating vector-borne diseases of humans and livestock in sub-Saharan Africa. Central to the pathogenesis of African trypanosomes is their transmission by the arthropod vector, Glossina spp. (tsetse fly). Intriguingly, the efficiency of parasite transmission through the vector is reduced following depletion of Trypanosoma brucei Procyclic-Specific Surface Antigen-2 ( TbPSSA-2). To investigate the underlying molecular mechanism of TbPSSA-2, we determined the crystal structures of its ectodomain and that of its homolog T. congolense Insect Stage Antigen ( TcISA) to resolutions of 1.65 Å and 2.45 Å, respectively using single wavelength anomalous dispersion. Both proteins adopt a novel bilobed architecture with the individual lobes displaying rotational flexibility around the central tether that suggest a potential mechanism for coordinating a binding partner. In support of this hypothesis, electron density consistent with a bound peptide was observed in the inter-lob cleft of a TcISA monomer. These first reported structures of insect stage transmembrane proteins expressed by African trypanosomes provide potentially valuable insight into the interface between parasite and tsetse vector. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
12. Structural and Functional Divergence of the Aldolase Fold in Toxoplasma gondii.
- Author
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Tonkin, Michelle L., Halavaty, Andrei S., Ramaswamy, Raghavendran, Ruan, Jiapeng, Igarashi, Makoto, Ngô, Huân M., and Boulanger, Martin J.
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ALDOLASES , *PARASITIC protozoa , *INTRACELLULAR pathogens , *TOXOPLASMA gondii , *DEOXYRIBOSE , *GLYCOLYSIS , *GENE expression - Abstract
Parasites of the phylum Apicomplexa are highly successful pathogens of humans and animals worldwide. As obligate intracellular parasites, they have significant energy requirements for invasion and gliding motility that are supplied by various metabolic pathways. Aldolases have emerged as key enzymes involved in these pathways, and all apicomplexans express one or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA). Intriguingly, Toxoplasma gondii , a highly successful apicomplexan parasite, expresses F16BP aldolase ( Tg ALD1), d5RP aldolase ( Tg DERA), and a divergent dR5P aldolase-like protein ( Tg DPA) exclusively in the latent bradyzoite stage. While the importance of Tg ALD1 in glycolysis is well established and Tg DERA is also likely to be involved in parasite metabolism, the detailed function of Tg DPA remains elusive. To gain mechanistic insight into the function of different T. gondii aldolases, we first determined the crystal structures of Tg ALD1 and Tg DPA. Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with divergent secondary structure elements. Structural comparison of Tg ALD1 and Tg DPA with members of their respective enzyme families revealed that, while the active-site residues are conserved in Tg ALD1, key catalytic residues are absent in Tg DPA. Consistent with this observation, biochemical assays showed that, while Tg ALD1 was active on F16BP, Tg DPA was inactive on dR5P. Intriguingly, both aldolases are competent to bind polymerized actin in vitro . Altogether, structural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization of the classic TIM barrel aldolase fold. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
13. Dissecting the molecular assembly of the Toxoplasma gondii MyoA motility complex.
- Author
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Powell CJ, Jenkins ML, Parker ML, Ramaswamy R, Kelsen A, Warshaw DM, Ward GE, Burke JE, and Boulanger MJ
- Subjects
- Animals, Calcium metabolism, Cell Movement, Crystallography, X-Ray, Nonmuscle Myosin Type IIA metabolism, Protein Binding, Protein Conformation, Protozoan Proteins metabolism, Toxoplasma growth & development, Nonmuscle Myosin Type IIA chemistry, Protozoan Proteins chemistry, Toxoplasma metabolism
- Abstract
Apicomplexan parasites such as Toxoplasma gondii rely on a unique form of locomotion known as gliding motility. Generating the mechanical forces to support motility are divergent class XIV myosins (MyoA) coordinated by accessory proteins known as light chains. Although the importance of the MyoA-light chain complex is well-established, the detailed mechanisms governing its assembly and regulation are relatively unknown. To establish a molecular blueprint of this dynamic complex, we first mapped the adjacent binding sites of light chains MLC1 and ELC1 on the MyoA neck (residues 775-818) using a combination of hydrogen-deuterium exchange mass spectrometry and isothermal titration calorimetry. We then determined the 1.85 Å resolution crystal structure of MLC1 in complex with its cognate MyoA peptide. Structural analysis revealed a bilobed architecture with MLC1 clamping tightly around the helical MyoA peptide, consistent with the stable 10 nm K
d measured by isothermal titration calorimetry. We next showed that coordination of calcium by an EF-hand in ELC1 and prebinding of MLC1 to the MyoA neck enhanced the affinity of ELC1 for the MyoA neck 7- and 8-fold, respectively. When combined, these factors enhanced ELC1 binding 49-fold (to a Kd of 12 nm). Using the full-length MyoA motor (residues 1-831), we then showed that, in addition to coordinating the neck region, ELC1 appears to engage the MyoA converter subdomain, which couples the motor domain to the neck. These data support an assembly model where staged binding events cooperate to yield high-affinity complexes that are able to maximize force transduction., (© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.)- Published
- 2017
- Full Text
- View/download PDF
14. Structural characterization reveals a novel bilobed architecture for the ectodomains of insect stage expressed Trypanosoma brucei PSSA-2 and Trypanosoma congolense ISA.
- Author
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Ramaswamy R, Goomeshi Nobary S, Eyford BA, Pearson TW, and Boulanger MJ
- Subjects
- Animals, Antigens, Protozoan genetics, Antigens, Protozoan metabolism, Protein Domains, Protozoan Proteins genetics, Protozoan Proteins metabolism, Trypanosoma brucei brucei genetics, Trypanosoma brucei brucei metabolism, Trypanosoma congolense genetics, Trypanosoma congolense metabolism, Tsetse Flies metabolism, Tsetse Flies parasitology, Antigens, Protozoan chemistry, Protozoan Proteins chemistry, Trypanosoma brucei brucei chemistry, Trypanosoma congolense chemistry
- Abstract
African trypanosomiasis, caused by parasites of the genus Trypanosoma, is a complex of devastating vector-borne diseases of humans and livestock in sub-Saharan Africa. Central to the pathogenesis of African trypanosomes is their transmission by the arthropod vector, Glossina spp. (tsetse fly). Intriguingly, the efficiency of parasite transmission through the vector is reduced following depletion of Trypanosoma brucei Procyclic-Specific Surface Antigen-2 (TbPSSA-2). To investigate the underlying molecular mechanism of TbPSSA-2, we determined the crystal structures of its ectodomain and that of its homolog T. congolense Insect Stage Antigen (TcISA) to resolutions of 1.65 Å and 2.45 Å, respectively using single wavelength anomalous dispersion. Both proteins adopt a novel bilobed architecture with the individual lobes displaying rotational flexibility around the central tether that suggest a potential mechanism for coordinating a binding partner. In support of this hypothesis, electron density consistent with a bound peptide was observed in the inter-lob cleft of a TcISA monomer. These first reported structures of insect stage transmembrane proteins expressed by African trypanosomes provide potentially valuable insight into the interface between parasite and tsetse vector., (© 2016 The Protein Society.)
- Published
- 2016
- Full Text
- View/download PDF
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