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When a steel frame is subjected to an abnormal load, a sudden local failure due to the loss of a load carrying member may result in progressive collapse of the entire structure. In this study, experiments and numerical simulations were performed to investigate the anti-collapse performance of double half-span assemblies (a column connected with two half-span beams) with welded unreinforced flange–bolted web (WUF-B) connections in a column removal scenario. Three types of bolted webs—two bolts in one row, three bolts in one row, and four bolts in two rows—were considered. For the post-peak response, the connections exhibited a significant resistance recovery owing to the catenary action. The bolt-bearing areas on the beam web were prone to fracture owing to excessive compression, and the failure modes of the web varied significantly with the bolt arrangement. The test results demonstrate that increasing the number of bolt rows is a fairly effective approach for enhancing the anti-collapse performance of the WUF-B connections. Numerical models were used to further investigate the effects of the bolt arrangement on the anti-collapse performance of the WUF-B connections. The results indicate that the bolt arrangement can directly affect the response of the connections after the first peak load, especially in the catenary phase. [ABSTRACT FROM AUTHOR]