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The Microwave Radiation Imager (MWRI) on board the FengYun-3A/B satellites observes the Earth atmosphere at 10.65, 18.7, 23.8, 36.5, and 89.0 GHz with each having dual polarization. Its calibration system is uniquely designed with a main reflector viewing both cold and hot calibration targets. Two quasi-optical reflectors are used to reflect the radiation from the hot load and cold space to the main reflector. In the MWRI calibration process, a radiation loss in the beam transmission path must be taken into account. The loss factor in the hot load transmission path is derived using the antenna pattern data measured on ground and satellite data observing over the Amazon forest where the scene temperature is steady and close to the hot load. The instrument nonlinearity factors at different channels are also evaluated over a wide range of brightness temperatures and compared with the results from the ground vacuum test. After a cross-calibration with Windsat data, atmospheric products are derived from MWRI brightness temperatures with the accuracy similar to those from the legacy sensors (e.g., the Special Sensor Microwave/Imager). [ABSTRACT FROM AUTHOR]