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BackgroundBovine paratuberculosis is a chronic infectious disease of ruminants primarily caused by Mycobacterium avium subsp. paratuberculosis (MAP). It is essentially a chronic granulomatous enteritis characterized by intractable diarrhea, progressive lethargy, and thickening of the intestinal mucosa with the formation of crumpled pouches. Bovine paratuberculosis not only adversely affects milk production and the quality of dairy products but also poses a significant threat to the economic development of dairy farming and human food security. This systematic review and meta-analysis was conducted to assess the prevalence of MAP infection among cattle herds in mainland ChinaResultsA total of 62 studies with data from 102,340 cattle in 24 provinces in China were selected after matching the assessment criteria. In China, the overall estimated prevalence of MAP infection in cattle was 8%(7727/102340). Interestingly, the MAP infection rate in cattle in southern China was estimated to be 2% (6/281), which was significantly lower compared with other regions of China, and the highest infection rate was 12% (1914/16008) in eastern China. MAP infection rates were related to age, average herd size, type of use, season, detection method, and sample type. Moreover, the MAP infection rate in cattle did not correlate with the publication date of the studies.ConclusionThe analysis identified age, average herd size, type of use, and season as significant potential risk factors associated with PTB pool positivity. In addition, the detection method and sample type can also potentially affect the incidence of detected PTB.

For the frequent occurrence of harmful algal blooms (HABs) in the Qinhuangdao coastal sea (QCS) of the Bohai Sea in summer, we tested the hypothesis that high-biodiversity HAB species exist in the area, and a series of censuses of HAB species were conducted in the QCS in the summers of 2014–2019. Through morphological identification, we found 100 algae species representing 42 genera in 3 phytoplankton phyla in this study, among which Bacillariophyta was the most dominant phylum. We also found that the population density of Dinoflagellata increased from 2016 to 2019. In total, 59 HAB species were annotated in this study, including 39 of Bacillariophyta, 18 of Dinoflagellata and 2 of Ochrophyta, of which 13 HAB species were reported in the Bohai Sea for the first time, and most HAB species were widely distributed in the QCS in summer. Notably, four dominant HAB species displayed unique temporal and spatial distribution characteristics, while their distribution ranges and population densities increased from 2014 to 2019. The distributions of five environmental factors were different in the QCS, while the temperature, salinity, and dissolved inorganic nitrogen might be the key environmental factors influencing the distribution of dominant HAB species in the summer. In conclusion, this study provides a detailed evaluation of phytoplankton diversity and interannual variation in the QCS. The existence of a high level of biodiversity of algal bloom species suggests the need for long-term monitoring in order to further study and prevent potential HABs. [ABSTRACT FROM AUTHOR]

Lacustrine shale, represented by the Middle Permian Lucaogou Formation in the Jimsar Depression in the eastern Junggar Basin, has become one of the main areas of shale oil exploration in China. In this study, we used 137 samples of shale from the Lucaogou Formation, drawn from 14 wells in the Jimsar Depression, to investigate their characteristics of pyrolysis, organic carbon and soluble organic matter content, biomarkers, organic microscopic composition, and vitrinite reflectance. Basin simulation and hydrocarbon generation thermal simulation experiments were also conducted in a closed system. The results of this study indicate that the input of an algae source was dominant in the source rocks of the Lucaogou Formation, that the water in which the rocks were deposited had high salinity and strong reducibility, and that the source rocks were oil-prone. The Lucaogou source rocks generally had good hydrocarbon generation capability, but showed significant heterogeneity. At the end of the Cretaceous period, the shales in the Lucaogou Formation entered the oil-generation window as a whole. Currently, the shales of the Lucaogou Formation are generally in the high-maturity stage in the deep part of the depression, producing a large amount of high-maturity oil and condensate gas, while those in the shallow part have relatively low maturity and can only produce a large amount of conventional crude oil. The maximum crude oil generation rate of the Lucaogou Formation shale obtained from the thermal simulation results was 220.2 mg/g of the total organic carbon (TOC), and the maximum hydrocarbon expulsion efficiency was estimated to be 59.3–76.4%. [ABSTRACT FROM AUTHOR]

The superposition of artificial intelligence (AI) and non-state power has aggravated changes in state functions, and has downgraded centralism of political state. The “Intelligent Leviathan” will persist in objectifying the “individual.” The relationship mode of “human-human” being transformed into the mode of “human)technology-human, ” is giving rise to a new social structure, while state power together with non-state power, law and the algorithm, as well as ethics and technologies, are creating a new order. The major threat to freedoms and rights is horizontal non-state power, instead of vertical state power. Going through profound changes, legal scenarios need reconstruction of “kindness” dimension for balancing the “rights priority” theory, while the “obligations standard” should be recovered in the sense of legal technology to rectify the “rights standard” and to resolve the “rights disability.” [ABSTRACT FROM AUTHOR]

A 6‐year‐old female bottlenose dolphin (Tursiops truncatus) kept in dolphinarium died after a 3.5‐month period of lethargy and inappetence despite antibiotics and supportive care. At necropsy, gross findings included diffuse varying‐sized nodules in the lungs and scattered nodules throughout the heart, spleen, mesenteric and hilar lymph node and kidney. Microscopically, the lesions were characterised by disseminated fungal pyogranulomas with numerous intralesional Mucor‐like fungi. The fungi structures were demonstrated by Periodic acid‐Schiff and Gomori methenamine silver stain. Molecular analyses of the fungi were Rhizopus microsporus by PCR sequencing 18S ribosomal RNA gene. Ziehl–Neelsen stain failed to show acid‐fast bacterial infection. Based on pathological and molecular examination, systemic granulomatous mucormycosis was diagnosed. To our knowledge, this is the first reported case of systemic mucormycosis caused by Rhizopus microsporus in bottlenose dolphin. [ABSTRACT FROM AUTHOR]

Mixed lineage leukemia protein (MLL1 protein) recognizes the CpG site via its CXXC domain and is frequently associated with leukemia. The specific recognition is abolished by C1188D mutation, which also prevents MLL‐related leukemia. In this paper, multiple molecular dynamic (MD) simulations were performed to investigate the mechanism of recognition and influences of C1188D mutation. Started from fully dissociated DNA and MLL1‐CXXC domain, remarkably, the center of mass (COM) of MLL1‐CXXC domain quickly concentrates on the vicinity of the CpG site in all 53 short MD simulations. Extended simulations of the wild type showed that the native complex formed in 500 ns among 4 of 53 simulations. In contrast, the C1188D mutant COM distributed broadly around the DNA and the native complex was not observed in any of the extended simulations. Simulations on the apo MLL1‐CXXC domain further suggest that the wild type protein remained predominantly in an open form that closely resembles its structure in the native complex whereas C1188D mutant formed predominantly compact structures in which the N‐ terminal bends to D1188. This conformational switch hinders the formation of encounter complex, thus abolishes the recognition. Our study also provides clues to the study mechanism of recognition, by the CXXC domain from proteins like DNA methyltransferase and ten‐eleven translocation enzymes. [ABSTRACT FROM AUTHOR]

Although the effective transmission of the H5N1 virus from humans to humans has yet to be further observed, humans are at increased risk of a pandemic caused by H5N1. In order to fully evaluate the seroprevalence and risk factor of highly pathogenic avian influenza A (H5N1) virus infection among in mainland China, we performed a systematic review and meta‐analysis. In this review, we searched literature on the seroprevalence of H5N1 infection among humans in mainland China from 1 January 1997 to 20 October 2018 in English and Chinese databases, including PubMed, Google scholar, Cochrane library, Clinical Trial, VIP, CNKI and WanFang database. We made a selection according to the title and the abstract of paper, and then, we excluded duplicated literature, and data incomplete literature according to the exclusion criteria we formulated. Finally, we extracted how many humans have H5N1 infection from the obtained studies to establish the seroprevalence of H5N1 infection among humans in mainland China. A total of 56 studies (including data of 35,159 humans) were compliant with our criteria. In China, the overall seroprevalence of H5N1 infection among humans was 2.45% (862/35,159), while the seroprevalence of H5N1 infection among humans from central China was 7.32% (213/2,911), higher than those in other regions of China. The seroprevalence of H5N1 infection was associated with test method, sampling time and demographic characteristics of humans. However, there was no significant difference in the effect of gender on the seroprevalence of H5N1 among humans in China. The purpose of this review was to better understand the real infection rate of H5N1 virus among humans and evaluate the potential risk factors for the zoonotic spread of H5N1 virus to humans. Sufficient epidemiological data are important to explore and understand the prevalent status of AIVs throughout the country and to disease control. [ABSTRACT FROM AUTHOR]

Appropriate flowering and maturity time are important for soybean production. Four maturity genes E1, E2, E3 and E4 have been molecularly identified and found to play major roles in the control of flowering and maturity of soybean. Here, to further investigate the effect of different allele combinations of E1-E4, we performed Kompetitive Allele Specific PCR (KASP) assays based on single nucleotide polymorphisms (SNPs) at these four E loci, and genotyped E1-E4 genes across 308 Chinese cultivars with a wide range of maturity groups. In total, twenty-one allele combinations for E1-E4 genes were identified across these Chinese cultivars. Various combinations of mutations at four E loci gave rise to the diversity of flowering and maturity time, which were associated with the adaptation of soybean cultivars to diverse geographic regions and farming systems. In particular, the cultivars with mutations at all four E loci reached flowering and maturity very early, and adapted to high-latitude cold regions. The allele combinations e1-as/e2-ns/e3-tr/E4, E1/e2-ns/E3/E4 and E1/E2/E3/E4 played important roles in the Northeast China, Huang-Huai-Hai (HHH) Rivers Valley and South China regions, respectively. Notably, E1 and E2, especially E2, affected flowering and maturity time of soybean significantly. Our study will be beneficial for germplasm evaluation, cultivar improvement and regionalization of cultivation in soybean production. [ABSTRACT FROM AUTHOR]

The aim of this study was to establish isothermal multiple self-matching initiated amplification (IMSA) and cross-priming amplification (CPA) methods to detect heat-stable I enterotoxin (STa)-producing enterotoxigenic Escherichia coli (ETEC). To avoid cross-contamination of aerosols, a closed independent isothermal amplification tube (IAT) was used to perform the assays. Optimal amplification conditions for IMSA and CPA were selected for specificity and sensitivity, respectively, and for clinical relevance. Both IMSA and CPA assays could specifically recognize all 3-STa positive strains in which they fluoresced green under UV light, but not in the 11 non-STa strains. The results of the sensitivity analysis indicated that the detection limit of the IMSA assay was 1.5 ×102 CFU, comparable to real-time PCR, but 10-fold more sensitive than CPA and LAMP. Further evaluation of the detection methods of swine diarrhea samples demonstrated that both could successfully identify the DNA of STa-producing ETEC in clinical specimens, consistent with LAMP and qPCR methods. The results demonstrated that the IMSA and CPA methods had high specificity and sensitivity with rapid detection of ETEC, so having great potential in clinical applications. [ABSTRACT FROM AUTHOR]

Wild birds are natural hosts of avian influenza viruses (AIV) and can transmit viruses to poultry and other species. To monitor the prevalence of AIV antibodies, 211 eggs from wild Mallards (Anas platyrhynchos) and 177 from wild White-winged Terns (Chlidonias leucopterus) were collected from Zhalong Wetland and Xianghai Wetland in northeastern Republic of China from April to September, 2016. A hemagglutinin inhibition test detected the presence of H1, H3, H5, and H7 subtype-specific antibodies. The prevalences of AIV antibodies of subtypes H1 and H3 were relatively high while the prevalences of H5 and H7 AIV subtype antibody were low. In Zhalong Wetland, the prevalence of H1 AIV subtype antibody in Mallards was the highest, with a percentage of 11.0%. Prevalence of all AIV subtype-specific antibodies in Mallards was higher than those in White-winged Terns. [ABSTRACT FROM AUTHOR]

Background: Respiratory syncytial virus (RSV) is an established cause of serious lower respiratory disease in infants, elderly and high-risk populations. The OUTSMART surveillance program aims to characterize patient populations and currently circulating RSV strains, and monitor temporal and geographic evolution of RSV F and G proteins in the U.S. Methods: The OUTSMART 2016–17 study collected RSV-positive samples from 25 RSVAlert® laboratories from 4 U.S. regions and Puerto Rico during November 2016 through March 2017. Frequencies of A and B subtypes and genotypes were determined for several demographic and geographic variables. To gauge the representativeness of the OUTSMART patients, results were compared to discharge data from the NEDS and NIS databases. Results: A total of 1,041 RSV-positive samples with associated demographic data were obtained and the RSV F gene and second variable region of the G gene were sequenced. The majority of samples (76.0%) came from children under 2 years old: <1 year (48.4%), 1–2 years (27.6%). The OUTSMART patient sample was similar to NEDS and NIS for age, gender, and geographic location. Both OUTSMART and national RSV cases peaked in January. Of OUTSMART samples, 45.3% were subtype A, 53.7% were subtype B and 1.0% were mixed A and B. The percentage of RSV B cases increased with increasing age. Hospitalization (length of hospital stay, LOS, >24 hrs) occurred in 29.0% of patients of which 52.0% had RSV B. Outpatients (LOS <24 hrs) were 64.4% of total of which 73.3% were diagnosed in the ER and discharged, while only 6% were diagnosed in other outpatient settings. Conclusions: The OUTSMART 2016–17 study was representative of the U.S. RSV experience. Geographic and temporal information from the RSV surveillance program will be used to establish a molecular baseline of RSV F and G sequence variability and to help inform development of novel agents for RSV prophylaxis and treatment. [ABSTRACT FROM AUTHOR]

Herein, we conducted a meta-analysis aimed at comprehensively assessing avian influenza A (H9N2) virus infection seroprevalence and infection-related risk factors among humans in China. We reviewed published studies pertaining to H9N2 seroprevalence of in China from inception to March 20, 2020, with PubMed, Clinical Trial, VIP, CNKI and databases being used to identify English and Chinese articles. After excluding the incomplete literature and data, 45 studies about risk factor of human H9N2 viral infections in China were analyzed by systematic review and meta-analysis. Our results showed that 45 studies (59,590 total patients) met the inclusion criteria. Overall H9N2 infection seroprevalence in China was estimated to be 5.56% (95%CI = 3.88–7.28), while that from central China the seroprevalence was 22.72% (95%CI = 12.18–33.84), with this prevalence being greater than that observed in other regions. H9N2 infection seroprevalence was related to sampling time, testing methodology, gender, and other demographic factors. This review will provide a basis for further understanding the risk factors of H9N2 infections in China, and it is necessary to study how to formulate strict and targeted measures to prevent the spread of the virus. • The data of 45 articles (including data on 59,590 humans) met the selection criteria in this meta-analysis. • The pooled seroprevalence of H9N2 infection among humans in China was 5.56%. • The seroprevalence of H9N2 infection among humans in different regions, and demographic characteristics, varied considerably. • Continuous surveillance the seroprevalence of H9N2 infection among humans in China is necessary. [ABSTRACT FROM AUTHOR]

• Cu induced oxidative stress and inhibited osteoblast mineralization. • Cu inhibited TGF-β1/Smad3 pathway in the osteoblast. • Cu toxicity inhibited osteoblast function through induction of the inflammation. To explore the relationship of oxidative stress and TGF-β 1/Smad3 pathway in the inhibition of osteoblast mineralization by copper chloride (CuCl 2), the osteoblasts were treated with CuCl 2 (0, 50 μM, 100 μM, 150 μM CuCl 2 5H 2 O) for 24 h. We found that Cu impaired the osteoblast structure, inhibited the glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, alkaline phosphatase (ALP) content, mRNA expression of collagen I (COL-I), osteocalcin (OCN), insulin-like growth factor I (IGF-I), bone morphogenetic protein-2 (BMP-2), transforming growth factor β1 (TGF-β1) and core-binding factor α1 (Cbfα1), promoted the reactive oxygen species (ROS) production, inactivated the TGF-β1/Smad3 pathway. It indicates that the inactivated TGF-β1/Smad3 pathway leads to osteoblast impairment by CuCl 2. It will contribute to clarify the influence of CuCl 2 on the osteoblast mineralization. [ABSTRACT FROM AUTHOR]

Over the past decade, various avian influenza viruses have been isolated from wild ducks found in the northeast of China. To monitor the prevalence of multiple subtype specific AIVs antibodies, 1705 wild ducks' eggs from six wetlands of northeast China were analyzed for surveillance of H1, H3, H5, and H7 AIVs antibodies by c-ELISA and HI test from Jan 2017 to Dec 2019. The results show that the combined frequency of multiple subtype specific AIVs antibodies were H1 (12.32%), H3 (8.15%), H5 (2.05%), and H7 (3.46%) respectively. Therefore, a detailed analysis of the geographical distribution of AIVs in China, and the risk factors for human infection is of vital importance. This study provides basic data for other researchers to deeply study AIVs distribution characteristics, and for governments to develop detailed measures to control the spread of AIVs. • This is first time long-term monitoring of multiple subtype antibodies of AIVs in egg yolk of wild duck in China. • The pooled prevalence of H1, H3, H5, and H7 subtype AIVs antibodies was 12.32%, 8.15%, 2.05%, and 3.46%, respectively. • The results showed that the wild ducks in northeast China had a risk for H5 and H7 subtype AIVs infection. [ABSTRACT FROM AUTHOR]

Nicotine as a major component of addiction in cigarettes has been reported to play protective roles in some pathological processes. It is reported that activation of the nicotinic acetylcholine receptor also has a cardioprotective effect. Thus, in our study, we investigated the effect and mechanism of nicotine on the autophagy of cardiomyocytes, and whether nicotine protects cardiomyocytes against palmitic acid (PA) injury. The results indicated that low-dose nicotine promoted neonatal mouse cardiac myocytes (NMCMs) autophagy and accelerated autophagic flux while inhibiting NMCMs apoptosis, but high-dose nicotine inhibited autophagy and promoted apoptosis. Moreover, low-dose nicotine upregulated heme oxygenase-1 (HO-1) expression and knocking down HO-1 abolished the effects of nicotine on the autophagy and apoptosis of NMCMs. Methyllycaconitine citrate (α7-nAChR blocker, MLA) inhibited HO-1 expression and the effects of nicotine on autophagy and apoptosis of NMCMs. Furthermore, low-dose nicotine improved the inhibited autophagy and increased apoptosis induced by palmitic acid (PA) in NMCMs and these effects were reversed by knocking down HO-1. In conclusion, our data suggested that low-dose nicotine promoted autophagy and inhibited apoptosis of cardiomyocytes by upregulating HO-1. • Different doses of nicotine have different effects on autophagy and apoptosis of cardiomyocytes. • Low-dose nicotine promotes autophagy and inhibits apoptosis in cardiomyocytes. • Low-dose nicotine regulates autophagy and apoptosis through α7-nAChRs/HO-1 pathway in cardiomyocytes. • Low-dose nicotine rescues the inhibition of autophagy and enhancement of apoptosis caused by palmitate. [ABSTRACT FROM AUTHOR]

Bovine herpesvirus 1 (BHV-1), an important pathogen of cattle, can cause severe clinical syndromes including respiratory disease, genital disease, and late-term abortions, as well as neurological and systemic disease in cattle. For assessing the prevalence of BHV-1 infection in mainland China, a systematic review and meta-analysis was conducted. In this systematic review and meta-analysis, we searched English and Chinese literature databases for published paper regarding the prevalence of BHV-1 in cattle in China from inception to May 20, 2018. Search strings included if they reported the cattle samples of more than 30 cattle and provided information that allowed us to establish the prevalence of BHV-1. Moreover, we excluded repeated studies, reviews, other hosts studies, as well as studies with inconsistent data, incomplete information or only provided prevalence data, and out of mainland China data. We extracted how many cattle have BHV-1 infection from the obtained studies, moreover, and calculated pooled prevalence of BHV-1 infection in cattle. The data of 41 articles (including data on 43,441 cattle) are compliant with the standards. The pooled prevalence of BHV-1 in cattle in China was 40%, the pooled prevalence of BHV-1 in cattle from Northeast China (24%) was significant lower than those from other regions. In addition, the prevalence of BHV-1 was associated with publication time of paper, detection methods, age of cattle, and clinical symptoms (pneumonia, abortion etc.).Our findings suggest that BHV-1 is common in cattle in mainland China. It is necessary to monitor the prevalence of BHV-1 in cattle and the powerful and effective regulatory measures should be taken out to prevent the spread of BHV-1. [ABSTRACT FROM AUTHOR]

Staphylococcus aureus is one of the most common mastitis-causing bacteria in dairy cows. It is associated with reduced production performance in animals and with huge financial losses for the dairy industry worldwide. An accurate and sensitive method for the early diagnosis and identification of Staph. aureus in milk samples is essential. The present study aimed to establish a closed-tube isothermal multiple self-matching-initiated amplification (IMSA) technique for visual confirmation of the presence of Staph. aureus targeting the nuc conserved sequence gene. The specific primers successfully amplified the target sequence within 45 min at 63°C reaction temperature and using the optimal components of the reaction system. The positive amplicon showed bright green fluorescence under UV light when mixed with the chromogenic substrate SYBR Green I, and the negative samples remained orange in color. We observed fluorescence and a ladder-like pattern in the IMSA amplicon for all Staph. aureus strains, and we observed no significant change for the non– Staph. aureus strains. The IMSA assay had high specificity compared with loop-mediated isothermal amplification (LAMP): it confirmed the presence of all 7 Staph. aureus strains, and we found no false-positive results for the 12 non– Staph. aureus strains. The lower limit of detection for the IMSA assay was 1 × 102 cfu/mL, 10-fold more sensitive than the results obtained using LAMP. We also successfully applied the IMSA assay to confirm the presence of Staph. aureus in milk samples of cows with mastitis, and the results were consistent with those of LAMP and real-time PCR. The present study reports the use of IMSA to confirm the presence of Staph. aureus and provides a potentially useful method for rapid preliminary screening for Staph. aureus. [ABSTRACT FROM AUTHOR]

Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants (POPs) that pose serious challenges to aquatic animals and environments. Compared with BDE-47 which was one of the most toxic congeners known to date, BDE-209 is less toxic with higher abundance in biotic and abiotic samples. In this study, we have explored the effects of BDE-47 and BDE-209 at different concentrations on the radical oxygen species (ROS) levels and the antioxidant defense system of Brachionus plicatilis. Antioxidant indexes were measured, including total protein content (TSP), the activities of antioxidant enzymes, lipid peroxidation and DNA damage. The results indicated that while low concentrations of PBDEs could activate the antioxidant defense mechanisms, prolonged exposure to higher concentrations of PBDEs could impair the antioxidative capacity of B.plicatilis (P < 0.05). The overwhelming of the B.plicatilis antioxidant defense mechanism led to an accumulation of free radicals, resulting in the overactivation of lipid peroxidation and the increased frequency of DNA damage (P < 0.05). By studying the toxicity of PBDEs and the detoxification mechanism of B.plicatilis , our research has revealed useful indexes for detecting and monitoring the level of BDE-47 and BDE-209 in the future. Altogether, this study holds immense value in the field of ecotoxicology and environmental safety and will aid in the proper management of PBDEs pollution. [Display omitted] • Minor PBDE exposure upregulates antioxidant activities and DNA repairs. • SeverePBDEexposurecausesantioxidative enzymedeficiency and releases radicals. • Excessive radicals inducelipid peroxidation and DNA damage. • PBDEs induce a cascade effect ofROS,TSP, antioxidative enzyme activity, lipid peroxidation and DNA integrity. • SOD, CAT and POD in rotifers are potential bioindicators of PBDE contamination. [ABSTRACT FROM AUTHOR]

Bovine rotavirus is the primary pathogen causing diarrhea in cattle and can be transmitted vertically through the placenta. It mainly presents with clinical signs such as depression, loss of appetite, diarrhea, vomiting, and dehydration. A systematic review and meta-analysis were conducted to assess the prevalence of BRV infection in mainland China. We conducted a literature search on the prevalence of BRV infection in pigs between Jan 1, 1979 and Dec 31, 2021 in English and Chinese databases, including PubMed, Google Scholar, Cochrane library, Clinical Trials, VIP, CNKI, and WanFang database. Selections were made based on the title and the abstract of the paper, Search strings included if they reported the cattle samples of more than 15 cattle and provided information that allowed us to establish the prevalence of BRV. Moreover, we excluded repeated studies, reviews, other hosts. Finally, we extracted the number of cattle with BRV infection from the obtained studies and provided information that permitted us to estimate the prevalence of BRV infection in cattle in mainland China. The data of 29 articles (including data on 10677 cattle) are compliant with the standards. The pooled prevalence of BRV in cattle in China was 46%（6635/10677）, the pooled prevalence of BRV in cattle from Northeast China (40%) was significantly lower than those from other regions. In addition, the prevalence of BRV was associated with publication time of paper, detection methods, age of cattle, and clinical symptoms（diarrhea, etc.). Our findings suggest that BRV infection is common among cattle in China. It is, therefore, necessary to carry out further research and monitor the prevalence of BRV infection. Furthermore, powerful and effective regulatory measures should be taken to prevent the transmission and spread of BRV among cattle populations. • The data of 29 articles met the selection criteria, and the prevalence of BRV was 46% in China. • Cattle's age was a risk factor associated with the prevalence rate of BRV. • This study may help to control treatment costs and overuse of antibiotics to improve food safety and public health. [ABSTRACT FROM AUTHOR]