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UNLABELLED: Fermentation of pectin-rich biomass by Saccharomyces cerevisiae can produce bioethanol as a fuel replacement to combat carbon dioxide emissions from the combustion of fossil fuels. Saccharomyces cerevisiae UCDFST 09-448 produces its own pectinase enzymes potentially eliminating the need for commercial pectinases during fermentation. This research assessed growth, pectinase activity, and fermentative activity of S. cerevisiae UCDFST 09-448 and compared its performance to an industrial yeast strain, S. cerevisiae XR122N. Saccharomyces cerevisiae UCDFST 09-448s growth was inhibited by osmotic stress (xylose concentrations above 1 M), ethanol concentrations greater than 5% v/v, and temperatures outside of 30°C-37°C. However, S. cerevisiae UCDFST 09-448 was able to consistently grow in an industrial pH range (3-6). It was able to metabolize glucose, sucrose, and fructose but was unable to metabolize arabinose, xylose, and galacturonic acid. The pectinase enzyme produced by S. cerevisiae UCDFST 09-448 was active under typical fermentation conditions (35°C-37°C, pH 5.0). Regardless of S. cerevisiae UCDFST 09-448s limitations when compared to S. cerevisiae XR122N in 15% w/v peach fermentations, S. cerevisiae UCDFST 09-448 was still able to achieve maximum ethanol yields in the absence of commercial pectinases (44.7 ± 3.1 g/L). Under the same conditions, S. cerevisiae XR122N produced 39.5 ± 3.1 g/L ethanol. While S. cerevisiae UCDFST 09-448 may not currently be optimized for industrial fermentations, it is a step toward a consolidated bioprocessing approach to fermentation of pectin-rich biomass. ONE-SENTENCE SUMMARY: Saccharomyces cerevisiae UCDFST 09-448 demonstrates the potential to ferment pectin-rich biomass as part of a consolidated bioprocess, but is sensitive to industrial stressors.

SUMMARY Vascular plant one‐zinc finger (VOZ) transcription factors (TFs) play crucial roles in plant immunity. Nevertheless, how VOZs modulate defense signaling in response to elicitor‐induced resistance is not fully understood. Here, the defense elicitor β‐aminobutyric acid (BABA) resulted in the visible suppression of Rhizopus rot disease of peach fruit caused by Rhizopus stolonifer. Defense priming by BABA was notably associated with increased levels of salicylic acid (SA) and SA‐dependent gene expression. Data‐independent acquisition proteomic analysis revealed that two VOZ proteins (PpVOZ1 and PpVOZ2) were substantially upregulated in BABA‐induced resistance (BABA‐IR). Furthermore, the interaction of PpVOZ1 and PpVOZ2 and their potential target of the TEOSINTE‐BRANCHED1/CYCLOIDEA/PCF (TCP)‐family protein PpTCP2 screened from protein–protein interaction networks was confirmed by yeast two‐hybrid (Y2H), luciferase complementation imaging and glutathione S‐transferase pull‐down assays. Furthermore, subcellular localization, yeast one‐hybrid, electrophoretic mobility shift assay and dual‐luciferase reporter assays demonstrated that nuclear localization of both PpVOZ1 and PpVOZ2 was critical for their contribution to BABA‐IR, as these proteins potentiated the PpTCP2‐mediated transcriptional activation of isochorismate synthase genes (ICS1/2). The overexpression of both PpVOZ1 and PpVOZ2 could activate the transcription of SA‐dependent genes and provide disease resistance in transgenic Arabidopsis. In contrast, the ppvoz1cas9 and ppvoz2cas9 loss‐of‐function mutations and the voz1cas9 voz2cas9 double mutation attenuated BABA‐IR against R. stolonifer. Therefore, the three identified positive TFs, PpVOZ1, PpVOZ2, and PpTCP2, synergistically contribute to the BABA‐activated priming of systemic acquired resistance in postharvest peach fruit by a VOZ‐TCP‐ICS regulatory module. [ABSTRACT FROM AUTHOR]

Taphrina deformans is a plant-pathogenic fungus and a responsible agent for causing peach leaf curl disease. Taphrina deformans affects peach fruit production and contributes to global economic losses. Commercial fungicides may provide temporary relief; however, their overuse resulted in adverse environmental consequences as well as led to drug-resistant strains of T. deformans. Therefore, the discovery of novel drug targets for the future synthesis of antifungal drugs against Taphrina deformans is needed. Here we studied Taphrina deformans by computational proteomics approaches. The whole genome and proteome of T. deformans were subjected to subtractive proteomics, high-throughput virtual screening, and molecular dynamic simulations. We employed subtractive proteomics analysis of 4,659 proteins extracted from UniProtKB database; after filtering out homologous and non-essential proteins, we identified 189 essential ones, including nine that participated in the crucial metabolic pathways of the pathogen. These proteins were categorized as nuclear (n = 116), cytoplasmic (n = 37), and membrane (n = 36). Of those essential proteins, glutamate–cysteine ligase (GCL) emerged as one promising target due to its essential function for glutathione biosynthesis process which facilitates T. deformans survival and pathogenicity. To validate GCL as an antifungal target, virtual screening and molecular docking studies with various commercial fungicides were carried out to better characterize GCL as a drug target. The data showed strong binding affinities for polyoxin D, fluoxastrobin, trifloxystrobin, and azoxystrobin within the active site of GCL. Polyoxin D showed a strong affinity when the measured docking score was at -7.34 kcal/mol, while molecular dynamics simulations confirmed stable interactions (three hydrogen bonds, two hydrophobic bonds, and one salt bridge interaction), supporting our findings that GCL represents an excellent target for antifungal drug development efforts. The results showed that GCL, as an innovative target for future fungicide designs to combat T. deformans infections , provides an avenue toward creating more effective peach leaf curl disease treatments while mitigating environmental harm caused by its current use. [ABSTRACT FROM AUTHOR]

Peach (Prunus persica L.) is one of the most important and oldest stone fruits grown in China. Though Diaporthe species have more commonly been reported as plant pathogens, endophytes and saprophytes with a wide range of plant hosts, little is known about the Diaporthe species associated with peach trunk diseases in China. In the present study, forty-four Diaporthe isolates were obtained from trees with peach branch canker, shoot blight and gummosis symptoms in four provinces in China. Based on a combination of morphology and multi-locus sequence analysis of the rDNA internal transcribed spacer region (ITS), calmodulin (cal), translation elongation factor 1-α (tef1) and β-tubulin (tub2), these Diaporthe isolates were assigned to four species. Detailed descriptions and illustrations of all of the species, D. arecae, D. caulivora, D. discoidispora and D. eres, are provided. This study further reports the first host association of D. caulivora and D. discoidispora on peaches worldwide. The pathogenicity experiment results revealed that D. arecae was the most aggressive species, whereas D. discoidispora was the least aggressive on detached peach shoots. This study provides new insights into the fungi associated with peach trunk diseases in China, and the results of this study may help to facilitate routine diagnosis and planning of suitable plant disease management strategies. [ABSTRACT FROM AUTHOR]

WRKY transcription factors play key roles in plant resistance to various stresses, but their roles in fruit ripening remain largely unknown. Here, we report a WRKY gene PpWRKY14 involved in the regulation of fruit ripening in peach. The expression of PpWRKY14 showed an increasing trend throughout fruit development. PpWRKY14 was a target gene of PpNAC1, a master regulator of peach fruit ripening. PpWRKY14 could directly bind to the promoters of PpACS1 and PpACO1 to induce their expression, and this induction was greatly enhanced when PpWRKY14 formed a dimer with PpNAC1. However, the transcription of PpNAC1 could be directly suppressed by two EIN3/EIL1 genes, PpEIL2 and PpEIL3. The PpEIL2/3 genes were highly expressed at the early stages of fruit development, but their expression was programmed to decrease significantly during the ripening stage, thus derepressing the expression of PpNAC1. These results suggested a PpEIL2/3–PpNAC1–PpWRKY14 module that regulates fruit ripening by modulating ethylene production in peach. Our results provided an insight into the regulatory roles of EIN3/EIL1 and WRKY genes in fruit ripening. [ABSTRACT FROM AUTHOR]

Small RNAs (sRNAs) control a wide range of development and physiological pathways in plants. To address the response of sRNA biogenesis to drought stress, we identified sRNA biogenesis genes, including 11 encoding argonautes (AGO), 8 encoding Dicer-like proteins (DCL), and 9 encoding RNA-dependent RNA polymerases (RDR) in the peach genome. Notably, the largest numbers of sRNA biogenesis genes are located to chromosome 1. The PAZ, PIWI, and MID domains were identified in PpAGOs, while the ribonuclease IIIa and IIIb domains were characterized in PpDCLs. The RDRP domain was recognized in PpRDRs. Orthologous similarity and collinearity analyses between Arabidopsis and peach revealed 5, 1, and 2 collinear blocks in AGOs, DCLs, and RDRs, respectively. Moreover, 41, 40, and 42 cis-acting elements were located in the promoters of PpAGOs, PpDCLs, and PpRDRs, respectively, with the majority related to drought stress response. Analysis of RNA sequencing (RNA-seq) data revealed that sRNA biogenesis genes were involved in drought stress response in different tissues. Furthermore, the expression of candidate genes was verified in two peach cultivars, Beijing 2-7 (BJ2-7) and Sinai (SN), which are tested as drought-tolerant and sensitive cultivars, respectively, based on the physiological and biochemical analyses, which revealed that the Chinese peach cultivar 'BJ2-7' exhibits greater drought resistance compared to the Egyptian peach cultivar 'SN'. Interestingly, the expression of PpAGO2b, PpDCL2b, PpDCL4, and PpRDR4 genes was induced in 'BJ2-7' but inhibited in 'SN' under drought stress. Overall, this study provides insight into the roles of sRNA biogenesis genes in response to drought stress in peach. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Peach (Prunus persica (L.) Batsch) trees are prone to heavy cropping, but crop load management options are limited. 1-aminocyclopropane-1-carboxylic acid (ACC) has been suggested to reduce crop load and improve fruit quality in peaches, but many questions remain concerning the role of endogenous ethylene in the abscission response and other side effects. Here, the use of ACC as a chemical thinner in peach trees was studied at different rates (350, 500, and 750 mg L−1) and timings [at full bloom (FB) and after petal fall (AP) when the fruit was approximately 15–20 mm in diameter] by comparing the results to those of an untreated control and a hand-thinning treatment as a reference. The abscission response and ethylene emission were related to the ACC concentration. ACC-induced ethylene production, as well as some degree of defoliation, was time-dependent, with the highest ethylene emission peaks and the lowest defoliation degree occurring when ACC was applied at FB. On the other hand, the intra-annual differences in the abscission response between the FBs and APs varied depending on the season. AP-treated fruits produced more endogenous ethylene than did untreated fruits up to harvest, which could have influenced fruit color. Finally, our results indicate that ACC in the range of 500 and 350 mg L−1 can be used in 'Flatbeauti' peaches at FB and AP, respectively, to induce adequate levels of fruit crop load without or with minor undesired effects. [ABSTRACT FROM AUTHOR]

Cold tolerance was measured midwinter in a New Hampshire, USA, orchard collection of 33 peach and nectarine cultivars over 3 years using natural and artificial freezing methods. Flower bud survival and xylem and cambium hardiness were based on the inflection point from nonlinear regression, as well as comparisons of injury at about 220 (C. Flower bud hardiness was greatest in ‘BuenOs’, ‘Contender’, ‘Cresthaven’, ‘Redhaven’, and ‘Scarlet Rose’. The lowest hardiness occurred in ‘Brigantine’, ‘Desiree’, ‘Emeraude’, ‘Evelynn’, ‘Galaxy’, ‘Glenglo’, ‘Jade’, ‘PF5D’, ‘PF23’, ‘Silverglo’, ‘Spring Snow’, and ‘Sugar May’. Other cultivars had either intermediate or inconsistent year-to-year bud hardiness. Bud hardiness in 2021 was weakly correlated with bud hardiness in 2023, but neither were correlated with bud mortality in 2022 after a severe freeze. Cambial hardiness in one year was not correlated with hardiness in other years. ‘August Rose’, ‘BuenOs’, ‘Cresthaven’, ‘Desiree’, and ‘Silverglo’ had the hardiest cambium which was consistent from year to year. Xylem hardiness was greatest in ‘BuenOs’, ‘Contender’, ‘Desiree’, ‘John Boy’, ‘PF17’, ‘Redhaven’, ‘Silvergem’, and ‘TangOs’. Xylem injury was significantly correlated across years indicating that this tissue responds more consistently to midwinter freezing temperatures than flower buds and cambium. [ABSTRACT FROM AUTHOR]

Peach production faces significant pre-harvest challenges, including low moisture, nutrient deficiencies, flower drop, physical damage, and surface discoloration, which can limit yield and fruit quality. To mitigate these issues, the present study hypothesized that foliar applications of silicon and zinc could enhance peach growth, yield, and quality due to their known roles in improving stress tolerance, nutrient uptake, and antioxidant activity. Therefore, this research aimed to identify optimal concentrations of silicon and zinc for quality peach production. Ten-year-old peach trees of uniform size were sprayed with four levels of silicon (0%, 0.1%, 0.2%, and 0.3%) and zinc (0%, 0.25%, 0.50%, and 0.75%) for two consecutive growing seasons, at the berry and pit hardening stages, using a Randomized Complete Block Design (RCBD) with three replications. The averaged data from the two years showed that the pre-harvest foliar application of silicon significantly improved all yield and quality attributes of peaches. The foliar application of silicon at 0.3% notably enhanced fruit growth, yield, and biochemical attributes. Additionally, the highest fruit growth, yield, and quality of peach fruits were observed at the 0.75% zinc concentration. Maximum antioxidant activity, flavonoid content, proline content, and catalase activity were observed in fruits from plants treated with 0.3% silicon, which were statistically on par with 0.2% silicon. However, peroxidase activity was highest at 0.2% silicon. Regarding zinc levels, antioxidant activity, flavonoid content, proline content, and peroxidase activity were highest in fruits treated with 0.75% zinc, while catalase activity was superior when fruits were sprayed with 0.50% zinc. The interaction between silicon and zinc concentrations was found to be non-significant for most parameters, except for titratable acidity, TSS–acid ratio, ascorbic acid content, antioxidant activity, flavonoid content, and peroxidase activity. In conclusion, the foliar application of 0.3% silicon and 0.75% zinc independently enhanced all yield and quality characteristics of peaches. For the agro-climatic conditions of Peshawar, 0.2% silicon and 0.50% zinc are recommended for optimal peach production. [ABSTRACT FROM AUTHOR]

As compared to conventional physical and chemical techniques, the biological method (green synthesis) is an economical, nontoxic, fast, eco-friendly and cost-effective technique that utilizes plant extract to generate highly stable and biocompatible nanoparticles. The existing study focuses on the synthesis of zinc sulphide nanoparticles (ZnS NPs) using P. persica leaf extract which is used as a reducing agent and their associated properties. The prepared samples were examined in terms of structural, optical, morphological and compositional properties. The SEM images showed the agglomerated spherical particles while XRD spectra confirmed the hexagonal (wurtzite) phase of synthesized ZnS samples with average crystallite sizes found to vary from 12.45 to 14.36 nm. The optical absorption spectra displayed the absorption peaks at 331 nm (4.26 eV) and 339 nm (4.10 eV). This blue shift in the absorption band suggests the formation of ZnS NPs compared with bulk ZnS (∼ 3. 6 8 eV) material. The FTIR peaks from 612– to 630 cm − 1 and 860– to 1022 cm − 1 are because of the symmetric bending vibrations of the Zn–S bands. Electrical measurements showed that the resistivity is found to be decreasing with increasing temperature, signifying the semiconducting behavior of the synthesized ZnS NPs. The findings of this study suggest the feasible use of P. persica leaves extract for the production of ZnS NPs which could be utilized as multifunctional material in medical applications, biosensors, photocatalytic and electrical applications. [ABSTRACT FROM AUTHOR]

The peach tree “Prunus persica” is widely cultivated in India, and its fruits which have been used for centuries in traditional Indian medicine, are also known as the Queen of the Fruits. Prunus persica has immense therapeutic potential, widespread use in folklore and ancient systems of medicine for its anti-urolithic and other medicinal properties, and is included in the Pashanbheda group of herbs mentioned in Ayurveda for the treatment of kidney stones. Moreover, with growing interest in phytopharmaceuticals in the present world, there always remains an urge for a comprehensive understanding of the phytochemicals responsible for the medicinal activity of herbal drugs, giving rise to the importance of conducting bioassay-guided fractionation. In this study, the anti-urolithiasis activity of different fractions of Prunus persica fruit extracts was evaluated via Nucleation, Aggregation, and Growth assays, and fraction III showed the highest effects in vitro. This study provides a pharmacological basis for using Prunus persica fruit extract in treating kidney stones. [ABSTRACT FROM AUTHOR]

Copyright of BIOAGRO is the property of Revista BIOAGRO and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Calcium foliar applications are known to effectively enhance peach quality; however, the optimal implementation strategy regarding fruit developmental stages and cultivars remains unclear. In this study, three different moments of fruit Ca applications in peach and nectarine are tested: Early season, Mid-season, and Late season. For this aim, the 44Ca isotope was used as a tracer, enabling the quantification and location of the Ca derived from the foliar fertilizer. Stone, flesh, and skin 44Ca enrichment was separately analyzed at harvest. The results indicate that Ca absorption in the fruits from external CaCl2 applications was influenced by the timing of the application during fruit development, with Late-season applications proving to be the most effective in increasing the Ca content in the fruit, corresponding with a higher fruit size at the application moment. Nevertheless, no differences in the absorption efficiency were found between the three timings of the application. Furthermore, the Ca from the foliar fertilizer in the fruit predominately remained in the flesh, followed by the skin. The Ca derived from the foliar fertilizer reached the stone in all of the experimental situations, but the Early- and Mid-season applications resulted in the highest amount of Ca derived from the fertilizer in this part of the fruit. Interestingly, the peach exhibited a higher Ca absorption efficiency compared to the nectarine, likely due to the presence of trichomes that retain the foliar fertilizer on the fruit surface. In conclusion, the Ca absorption and distribution in peaches depends on the cultivar and timing of the Ca application. [ABSTRACT FROM AUTHOR]

In the Vase system, the most common training system for peach-growing countries for more than a century, light distribution to the canopy is uneven, and access to the canopy for pruning, thinning, and harvest labor is difficult. It is important to identify alternative systems to the Vase system considering the cultivar and growing environment to facilitate labor and enhance productivity and quality. In Türkiye, one of the important centers of peach growing worldwide, detailed research has yet to be published on the applicability of training systems alternative to the widely used Vase system. Therefore, this study aimed to evaluate the effect of different training systems (Vase, Catalan Vase, Quad-V, Tri-V) on growth, yield, fruit quality, and labor costs of peach cultivars (ExtremeVR 314, ExtremeVR 436, ExtremeVR 568). The experiment was conducted from 2017 to 2022. Although the distance between rows in all training systems is 5 m, the distance between trees on the row is determined as 4 m in Vase, 3 m in Catalan Vase, 2.5 m in Quad-V, and 2 m in Tri-V. In the experiment, vegetative development parameters, such as canopy volume, trunk sectional area, and the amount of winter pruning weights, differed according to the training system. In the final year, the Vase system, which produces the most pruning weight, generates 48.0% more pruning weight compared with the Tri-V system, which produces the least. Concerning yield per tree and hectare, trained to the Vase system yielded higher fruit per tree regardless of cultivar, while the Quad-V and Tri-V systems yielded more fruit per hectare. The training system and cultivar affected the fruit size; the largest fruits were obtained from the ExtremeVR 568 cultivar trained according to the Vase system. The most time needed for winter pruning was obtained from the Vase (79.4 min/tree) system, and the Tri-V (57.4 min/tree) and Quad-V (60.3 min/tree) systems required the least time. The Catalan Vase (31.1 min/tree) system required the least time for summer pruning. The most fruit harvest in an hour was obtained from the trees trained according to the Tri-V (164.5 kg/h) and Quad-V (132.02 kg/h) systems. These results suggest that Quad-V and Catalan Vase systems performed well and could be alternatives to the Vase system. [ABSTRACT FROM AUTHOR]

The current study was performed on a 6-year-old "Florida" peach cultivar (Prunus persica) during 2022 and 2023 respectively. Twenty-four peach trees uniformly were selected and sprayed two times at the beginning of pit starts hardening and at the end of phase two of fruit growth with the following treatments: the control, glycine betaine at 400 mg l-1, potassium silicate at 200 mg l-1, potassium silicate at 400 mg l-1, potassium silicate at 200 mg l-1 + glycine betaine at 400 mg l-1, potassium silicate at 400 mg l-1 + glycine betaine at 400 mg l-1, proline at 400 mg l-1 and potassium silicate at 200 mg l-1 + proline at 400 mg l-1. The results indicated that both treatments of potassium silicate at 200 mg l-1 + proline at 400 mg l-1 and potassium silicate at 400 mg l-1 + glycine betaine at 400 mg l-1 resulted in a significant increase in concentrations of anthocyanin and total carotenoid contents in the skin. Moreover, total soluble solid, vitamin C and total soluble solid / acidity were shown with high concentrations as compared with the control. Overall, the use of potassium silicate at 200 mg l-1 + proline at 400 mg l-1 or potassium silicate at 400 mg l-1 + glycine betaine at 400 mg l-1 two times is recommended. [ABSTRACT FROM AUTHOR]

'BRS Sarau' is a highly productive peach cultivar widely adapted to the Southern and Southeast region of Brazil. It produces fruits of excellent flavor and shape, with attractive skin and flesh color, and its harvest starts near the Christmas holidays, when the demand for peaches is high. [ABSTRACT FROM AUTHOR]

In the period from 2020 - 2022 on the territory of the Fruit-Growing Institute – Plovdiv a study was conducted to determine the impact of climate change on phenological development. Observations were carried out on 12 peach cultivars, 5 of which were indigenous and the rest introduced. In 2020, the phenological development started between 15 and 19 February, the earliest flowering occurred in the cultivars Filina and Elegant Lady on 14 March and on 25 March in the cultivar Evmolpia. In 2021, mass bud development was recorded from 1 to 5 March, the latest cultivar to enter this phase was Evmolpia on 10 March. The onset of flowering was recorded from 30 March to 2 April. In 2022, bud swell was recorded earliest in the Maycrest cultivar on 3 March and latest in the Evmolpia cultivar on 7 March. The phase of the beginning of flowering was first established in the cultivars Flavia, Filina, Elegant Lady, Ruby Rich and Royal Jam on 29 March. In the other cultivars observed, the same phenological phase occurred between 30 and 31 March. [ABSTRACT FROM AUTHOR]

Pattern-triggered immunity (PTI) is a critical defense mechanism employed by plants against pathogen attacks. This study explores the role of PTI induced by the Xyn11/eix fungal elicitor in two commercially valuable Rosaceae species, Prunus persica (peach) and Prunus avium (sweet cherry). Our findings demonstrate that Xyn11/eix triggers two specific defense responses: the increase in ethylene production and the induction of cell death. Furthermore, Xyn11/eix-mediated PTI significantly reduces the susceptibility to Botrytis cinerea infection in both species. The study reveals changes in gene expression patterns after Xyn11/eix treatment. Notably, ACO1 and SARDEF1 genes, involved in ethylene and salycilic acid biosynthesis, respectively, are upregulated in P. persica, but not in P. avium at the time point analyzed. This result suggests a potential role for the ethylene and salicylic acid signaling in Xyn11/mix-mediated PTI in P. persica. Additionally, the research identified functional orthologues of LeEIX2, the receptor for Xyn11/eix in Solanum lycopersicum, within both Prunes genomes. Altogether, these results suggest a remarkable functional convergence between Rosaceae and Solanaceae plants in the Xyn11/eix mediated defense responses although not at the transcriptional level, and opens new avenues for developing novel disease control strategies for stone fruits.

Taphrina deformans is a plant-pathogenic fungus and a responsible agent for causing peach leaf curl disease. Taphrina deformans affects peach fruit production and contributes to global economic losses. Commercial fungicides may provide temporary relief; however, their overuse resulted in adverse environmental consequences as well as led to drug-resistant strains of T. deformans. Therefore, the discovery of novel drug targets for the future synthesis of antifungal drugs against Taphrina deformans is needed. Here we studied Taphrina deformans by computational proteomics approaches. The whole genome and proteome of T. deformans were subjected to subtractive proteomics, high-throughput virtual screening, and molecular dynamic simulations. We employed subtractive proteomics analysis of 4,659 proteins extracted from UniProtKB database; after filtering out homologous and non-essential proteins, we identified 189 essential ones, including nine that participated in the crucial metabolic pathways of the pathogen. These proteins were categorized as nuclear (n = 116), cytoplasmic (n = 37), and membrane (n = 36). Of those essential proteins, glutamate–cysteine ligase (GCL) emerged as one promising target due to its essential function for glutathione biosynthesis process which facilitates T. deformans survival and pathogenicity. To validate GCL as an antifungal target, virtual screening and molecular docking studies with various commercial fungicides were carried out to better characterize GCL as a drug target. The data showed strong binding affinities for polyoxin D, fluoxastrobin, trifloxystrobin, and azoxystrobin within the active site of GCL. Polyoxin D showed a strong affinity when the measured docking score was at -7.34 kcal/mol, while molecular dynamics simulations confirmed stable interactions (three hydrogen bonds, two hydrophobic bonds, and one salt bridge interaction), supporting our findings that GCL represents an excellent target for antifungal drug development efforts. The results showed that GCL, as an innovative target for future fungicide designs to combat T. deformans infections, provides an avenue toward creating more effective peach leaf curl disease treatments while mitigating environmental harm caused by its current use.

To prevent frost damage in fruit trees, growers employ passive and active methods, and one of these second methods is the use of biostimulant compounds against abiotic stress. In this study, two trials were conducted to evaluate the effectiveness of a multi-attribute approach biostimulant—containing α-tocopherol, boron, and glycols, in peach ('UFO-4' cultivar) and almond ('Vairo' cultivar) trees. In a first trial, one-year-old shoots with flowers were collected after 24 h, 48 h, and 96 h of the biostimulant applications. Two different application rates of the product (1000 and 2000 cc ha−1) were tested and compared to an untreated control. In a second trial, one-year-old shoots with fruitlets were collected after 24 h of the biostimulant applications. In this case, only an application rate (2000 cc ha−1) was tested. In the two trials, the collected one-year-old shoots were subjected to different frost temperatures using a controlled environment chamber. The damage level was assessed by a morphological analysis of the flowers and fruitlets 96 h after each frost cycle simulation. The lethal temperatures (LT10, LT50, and LT90) of each treatment were calculated by probit analysis. The product applied 24 h and 48 h before the frost simulations significantly decreased the LT10 and LT50 in 1.5 °C in peach flowers, and 2.5 °C in almond flowers (a temperature reduction of 50% and 75%, respectively). These results were more consistent when the application volume was 2000 cc ha−1, instead of 1000 cc ha−1. Significant differences between treated and non-treated fruitlets were observed only in almond fruitlets, with LT10 and LT50 being 0.5 °C lower in treated fruitlets (20% reduction). In conclusion, the multi-attribute approach biostimulant applied 24 or 48 h before the frost reduced the mortality of peach and almond flowers, but its effectiveness to protect fruitlets after bloom was inconsistent. [ABSTRACT FROM AUTHOR]

Peaches are susceptible to various environmental stresses. Particularly in late spring, freezing temperatures can damage peaches and consequently, affect their productivity. Therefore, flowering delay is a prominent strategy for avoiding spring frost damage. Our previous study confirmed that treatment with 5% sodium alginate and 100 mM CaCl2 (5AG) to avoid frost damage during the blooming stage delays flowering. To reveal the flowering delay mechanism of peaches, this study systematically analyzed the modification of amino acid profiles in control and 5AG-treated peach plants at different day intervals. Our findings indicate that arginine (Arg), glutamate (Glu), and proline (Pro) levels differed between the control and 5AG-treated peach shoots throughout the phenological development of flower buds. Furthermore, two amino acids (Arg and Glu) are involved in the Pro pathway. Thus, using a computational metabolomics method, Pro biosynthesis and its characteristics, gene ontology, gene synteny, cis-regulatory elements, and gene organizations were examined to decipher the involvement of Pro metabolism in peach flowering delay. In addition, qRT-PCR analysis revealed the transcriptional regulation of Pro-related and flowering-responsive genes and their role in flowering delay. Overall, this pilot study provides new insights into the role of Pro in the flowering delay mechanisms in Prunus persica through 5AG treatment. [ABSTRACT FROM AUTHOR]

Nectarines can be affected by many diseases, resulting in significant production losses. Natural products, such as essential oils (EOs), are promising alternatives to pesticides to control storage rots. This work aimed to test the efficacy of biofumigation with EOs in the control of nectarine postharvest diseases while also evaluating the effect on the quality parameters (firmness, total soluble solids, and titratable acidity) and on the fruit fungal microbiome. Basil, fennel, lemon, oregano, and thyme EOs were first tested in vitro at 0.1, 0.5, and 1.0% concentrations to evaluate their inhibition activity against Monilinia fructicola. Subsequently, an in vivo screening trial was performed by treating nectarines inoculated with M. fructicola, with the five EOs at 2.0% concentration by biofumigation, performed using slow-release diffusers placed inside the storage cabinets. Fennel, lemon, and basil EOs were the most effective after storage and were selected to be tested in efficacy trials using naturally infected nectarines. After 28 days of storage, all treatments showed a significant rot reduction compared to the untreated control. Additionally, no evident phytotoxic effects were observed on the treated fruits. EO vapors did not affect the overall quality of the fruits but showed a positive effect in reducing firmness loss. Metabarcoding analysis showed a significant impact of tissue, treatment, and sampling time on the fruit microbiome composition. Treatments were able to reduce the abundance of Monilinia spp., but basil EO favored a significant increase in Penicillium spp. Moreover, the abundance of other fungal genera was found to be modified. [ABSTRACT FROM AUTHOR]

This study was carried out to investigate fruit yield and quality characteristics among new nectarine cultivars grown in the eastern Mediterranean region of Turkey. 'Gardeta', 'Gartario', and 'Garofa' nectarine cultivars were cultivated on 'GN15' rootstock. The flowering stage, fruit set percentage, yield, and fruit quality properties of these cultivars were investigated between 2018 and 2021. During the study, chill requirements ranged between 391 and 600 chilling hours and between 207 and 361 chill units in the area, and huge values were obtained for the average initial fruit set above 60% and the final fruit set above 45%. 'Gardeta' had the highest cumulative yield per tree (80.15 kg tree-1) and cumulative yield per hectare (133.04 tons ha-1). The fruit size, Total Soluble Solids (TSS) content, and fruit coloration were homogeneously distributed in all three cultivars. In addition, the Flowering (F) and Initial Fruit Set (IFS) characters were negatively correlated with Fruit Weight (FW), Fruit Length (FLE), and Fruit Diameter (FD). As a result, the 'Gardeta' was found remarkable with late flowering, the highest yield, and superior fruit quality characteristics such as size, red skin color, and high TSS/total acidity values in the eastern Mediterranean region of Turkey. In addition, the date of Full Flowering (FF) and Fruit Firmness (FF) were negatively correlated with fruit Skin color L* (SL), C (SC), and h° (SH) variables. The results demonstrated that the new nectarine cultivars used here showed changes in yield parameters and fruit quality attributes depending on the genotypic and environmental effects. [ABSTRACT FROM AUTHOR]

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[Objective]To understand the effect of different fertilizers and different fertilization amount on the growth and SPAD of container Prunus persica L. and provide optimal fertilization method for high quality peach plants. [Method]Thirteen different fertilization treatments were designed to study the plant height, ground diameter, crown breadth, branch numbers, the length of current-growth branches and SPAD of container Prunus persica during different periods. [Result]Compound fertilizer of balanced type was more suitable for plant growth than humate fertilizer of phosphorus and potassium type. Combination application of balanced compound fertilizer and low concentration EM bacteria was more beneficial for the growth of container Prunus persica single compound fertilizer. [Conclusion]The optimal treatment for the growth of container Prunus persica was to apply nitrogen, phosphorus and potassium balanced compound fertilizer (20 ∶ 20 ∶ 20) with 1 ∶ 1 000-1 ∶ 500 concentration and EM bacteria with 1 ∶ 200 concentration during growing period. [ABSTRACT FROM AUTHOR]

Recent studies have opened the way for using elicitor‐induced resistance in plants as a method to control arthropod pests. In this study, 1,3‐β‐glucan laminarin, an elicitor of disease resistance in plants, was tested on the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), on peach [Prunus persica (L.) Batsch, Rosaceae] plantlets and evaluated its effects on short‐term mortality and population growth. Laminarin exposure did not affect aphid survival in the short term; however, laminarin‐treated peach plants sustained fewer nymphs and adults in comparison with the control. Aphid populations on plants treated with laminarin declined significantly over the sampling period compared to the control. Moreover, the demographic parameters net reproductive rate (R0), finite rate of increase (λ), and intrinsic rate of increase (rm), all showed decreasing trends in aphid populations reared on laminarin‐treated plants. The decline in aphid populations exposed to laminarin seemed to mainly be linked to reduced adult survival, slower nymph development, and lower nymph survival and only marginally to changes in reproduction outcome. Changes in gene expression causing the final production of defence chemicals by peach plants may contribute to explaining the results. However, potential direct effects of laminarin on M. persicae feeding activity and probing behaviour cannot be ruled out. This study provides evidence that, although laminarin did not display insecticidal activity in the short term, this elicitor caused sublethal effects, significantly reducing aphid populations. [ABSTRACT FROM AUTHOR]

In northern temperate zones, peach trees are vulnerable to cold temperature injury in the fall, particularly as climate change prolongs warm weather in the fall and potentially delays the onset of cold acclimation. Four experiments evaluated how cold acclimation of flower buds and shoot phloem, cambium, and xylem is affected by exposure to varying temperatures in the fall. One-year-old peach shoots from trees grown in Maine, USA, were collected from October through November, exposed to 1, 3, or 6 days of low, high, and freezing temperatures, and subjected to stepwise controlled freezing to about −30 °C. Injury was visually quantified as oxidative browning of flower buds and shoot tissues. High temperature exposure, even of a single day, decreased cold tolerance of flower buds and shoot tissues until late November, when high temperatures only minimally decreased cold hardiness. In mid-November, increasing the duration of high temperature exposure from 1 to 3 days decreased cambium and phloem hardiness, but hardiness in flower buds was not further decreased by the longer duration of 3 days. By late November, hardiness in flower buds, cambium, and phloem was less responsive to high temperature, and was increased by prior exposure to 6 days of freezing. After high temperature, xylem lost hardiness to a small degree in mid-October and late November, but in mid-November this occurred in only one experiment. In this study, deacclimation during high temperature in the fall was greater in cambium and phloem than in flower buds and at times greater than in xylem. [ABSTRACT FROM AUTHOR]

Abstract The objective of this work was to quantify peach (Prunus persica) tree production in the Y-training and open-vase management systems, subjected to three summer pruning methods. The experiment was set up in a 2x3 factorial arrangement, with two management systems and three summer pruning methods (no summer pruning, summer pruning, and branch bending). The plants were arranged in two spacings: 1.5×5.0 m for the Y-training system, with 1,334 plants per hectare; and 4.0×5.0 m for open-vase system, with 500 plants per hectare. In the early summer of 2019, summer pruning was performed, being repeated in the three following years. In the branch bending treatment, new branches were twisted manually until reaching an angle of 110°. In the treatment without summer pruning, the branches were not removed. The Y-training system increases peach production. The methods of branch bending in summer and no prunning in summer provide an equivalent peach production, whereas the summer pruning treatment results in the lowest production.

In the Vase system, the most common training system for peach-growing countries for more than a century, light distribution to the canopy is uneven, and access to the canopy for pruning, thinning, and harvest labor is difficult. It is important to identify alternative systems to the Vase system considering the cultivar and growing environment to facilitate labor and enhance productivity and quality. In Türkiye, one of the important centers of peach growing worldwide, detailed research has yet to be published on the applicability of training systems alternative to the widely used Vase system. Therefore, this study aimed to evaluate the effect of different training systems (Vase, Catalan Vase, Quad-V, Tri-V) on growth, yield, fruit quality, and labor costs of peach cultivars (Extreme® 314, Extreme® 436, Extreme® 568). The experiment was conducted from 2017 to 2022. Although the distance between rows in all training systems is 5 m, the distance between trees on the row is determined as 4 m in Vase, 3 m in Catalan Vase, 2.5 m in Quad-V, and 2 m in Tri-V. In the experiment, vegetative development parameters, such as canopy volume, trunk sectional area, and the amount of winter pruning weights, differed according to the training system. In the final year, the Vase system, which produces the most pruning weight, generates 48.0% more pruning weight compared with the Tri-V system, which produces the least. Concerning yield per tree and hectare, trained to the Vase system yielded higher fruit per tree regardless of cultivar, while the Quad-V and Tri-V systems yielded more fruit per hectare. The training system and cultivar affected the fruit size; the largest fruits were obtained from the Extreme® 568 cultivar trained according to the Vase system. The most time needed for winter pruning was obtained from the Vase (79.4 min/tree) system, and the Tri-V (57.4 min/tree) and Quad-V (60.3 min/tree) systems required the least time. The Catalan Vase (31.1 min/tree) system required the least time for summer pruning. The most fruit harvest in an hour was obtained from the trees trained according to the Tri-V (164.5 kg/h) and Quad-V (132.02 kg/h) systems. These results suggest that Quad-V and Catalan Vase systems performed well and could be alternatives to the Vase system.

La mosca del mediterráneo Ceratitis capitata es una plaga que afecta el durazno en el nororiente colombiano. Aunque se han llevado a cabo estudios de caracterización y zonificación del sistema productivo en Boyacá y Norte de Santander, no hay información reciente sobre el conocimiento y manejo de la mosca del mediterráneo en este cultivo. Con el objetivo de identificar las principales características de los productores, manejo de cultivo y de C. capitata, en Boyacá, Santander y Norte de Santander, en 2021 se aplicaron 128 encuestas con 92 preguntas y una tasa de respuesta del 96%, los datos recolectados se sometieron a análisis de correspondencia múltiple y conglomerados. En los análisis se evidenció la conformación de tres grupos o tipologías: GI – Boyacá: zona de menor infestación de mosca, predominan lotes rodeados de durazno, topografía plana y ondulada; productores conocen la plaga, no identifican el daño, no saben calcular el índice Mosca Trampa Día (MTD). GII – Norte de Santander: zona de mayor infestación, predomina la topografía mixta, durazneros y otros caducifolios; cosechan según calendario, no realizan monitoreo, ni control de plagas; saben estimar el MTD, pero no lo usan para manejar la mosca. GIII – Santander: zona de infestación media, plantaciones rodeadas de durazno y otros cultivos; conocen la mosca, el daño, saben calcular MTD y lo utilizan para implementar prácticas de manejo. Estos resultados indican la necesidad de generar estrategias diferenciales para la transferencia de tecnología con el fin de tener una mayor tasa de adopción del manejo integrado de mosca

Abstract The notorious woody plant-degrading pathogen Lasiodiplodia theobromae is a major causal agent of peach gummosis, one of the prevalent and devastating trunk diseases to peach production; however, its pathogenesis is largely unknown. Our previous study showed that L. theobromae LtGPX3, which encodes a glutathione peroxidase resembling yeast GPX3/HYR1-like, was constantly and dramatically upregulated at the infectious stages. Here, we functionally characterized LtGPX3 using the CRISPR-Cas9-aided split marker approach. The ΔLtgpx3 deletion mutants displayed increased sensitivity to the osmotic stress agent KCl and less sensitivity to the cell wall-damaging agent calcofluor white. Exogenous oxidants highly induced the expression of LtGPX3, and the ΔLtgpx3 mutants displayed increased sensitivity to ROS-generating oxidants. Pathogenicity assays revealed that ΔLtgpx3 mutants showed compromised virulence in peach shoots, which was partially restored when peach shoots were pretreated with an NADPH oxidase inhibitor before inoculation. Moreover, ROS levels were strongly boosted, and transcripts of plant defense-related genes were highly induced in the ΔLtgpx3 mutants-infected peach shoots compared with the wild-type-inoculated. Overall, our results showed the essential roles of LtGPX3 in the oxidative stress response and tolerance and pathological functions in L. theobromae. These findings deepen our understanding of the survival strategies of the woody plant-degrading pathogen L. theobromae and provide new insights into developing new strategies for peach gummosis disease control.

Small RNAs (sRNAs) control a wide range of development and physiological pathways in plants. To address the response of sRNA biogenesis to drought stress, we identified sRNA biogenesis genes, including 11 encoding argonautes (AGO), 8 encoding Dicer-like proteins (DCL), and 9 encoding RNA-dependent RNA polymerases (RDR) in the peach genome. Notably, the largest numbers of sRNA biogenesis genes are located to chromosome 1. The PAZ, PIWI, and MID domains were identified in PpAGOs, while the ribonuclease IIIa and IIIb domains were characterized in PpDCLs. The RDRP domain was recognized in PpRDRs. Orthologous similarity and collinearity analyses between Arabidopsis and peach revealed 5, 1, and 2 collinear blocks in AGOs, DCLs, and RDRs, respectively. Moreover, 41, 40, and 42 cis-acting elements were located in the promoters of PpAGOs, PpDCLs, and PpRDRs, respectively, with the majority related to drought stress response. Analysis of RNA sequencing (RNA-seq) data revealed that sRNA biogenesis genes were involved in drought stress response in different tissues. Furthermore, the expression of candidate genes was verified in two peach cultivars, Beijing 2-7 (BJ2-7) and Sinai (SN), which are tested as drought-tolerant and sensitive cultivars, respectively, based on the physiological and biochemical analyses, which revealed that the Chinese peach cultivar ‘BJ2-7’ exhibits greater drought resistance compared to the Egyptian peach cultivar ‘SN’. Interestingly, the expression of PpAGO2b, PpDCL2b, PpDCL4, and PpRDR4 genes was induced in ‘BJ2-7’ but inhibited in ‘SN’ under drought stress. Overall, this study provides insight into the roles of sRNA biogenesis genes in response to drought stress in peach.

Peach (Prunus persica L.) is one of the most important and oldest stone fruits grown in China. Though Diaporthe species have more commonly been reported as plant pathogens, endophytes and saprophytes with a wide range of plant hosts, little is known about the Diaporthe species associated with peach trunk diseases in China. In the present study, forty-four Diaporthe isolates were obtained from trees with peach branch canker, shoot blight and gummosis symptoms in four provinces in China. Based on a combination of morphology and multi-locus sequence analysis of the rDNA internal transcribed spacer region (ITS), calmodulin (cal), translation elongation factor 1-α (tef1) and β-tubulin (tub2), these Diaporthe isolates were assigned to four species. Detailed descriptions and illustrations of all of the species, D. arecae, D. caulivora, D. discoidispora and D. eres, are provided. This study further reports the first host association of D. caulivora and D. discoidispora on peaches worldwide. The pathogenicity experiment results revealed that D. arecae was the most aggressive species, whereas D. discoidispora was the least aggressive on detached peach shoots. This study provides new insights into the fungi associated with peach trunk diseases in China, and the results of this study may help to facilitate routine diagnosis and planning of suitable plant disease management strategies.

Climate change is affecting the production of temperate fruit crops. Freeze damage, particularly in spring, has resulted in significant economic losses in peach production in the southeastern United States. Research efforts in peach and other Prunus species have primarily focused on dormancy-related traits associated with bloom time, such as chill and heat requirement, with fruitlet freeze tolerance not equally represented. This study reports fruitlet freeze tolerance in 75 peach and nectarine accessions at six freezing temperatures (0 to −10 °C) using electrolyte leakage method over two seasons (2022–2023). Fruitlet freeze tolerance ranged from −3.9 to −10.2 °C with an estimated freeze damage ranging from 16–48% of fruitlet tissue with the majority of the accessions showing tolerance to cold temperatures in the −4 to −6 °C and 25–35% range. Variability in tolerance was noted across years, including some inconsistencies between tolerance group assignments. Grouping based on the estimated damage showed better stability and some accessions changed their grouping from the extremes to an intermediate tolerance group. Interestingly, nectarine accessions were among the most tolerant in both seasons. Broad-sense heritability of 0.52 and 0.85, estimated for freeze tolerance and % tissue damage, respectively, suggested genetic control of this trait with a potential for improvement via breeding. [ABSTRACT FROM AUTHOR]

The notorious woody plant-degrading pathogen Lasiodiplodia theobromae is a major causal agent of peach gummosis, one of the prevalent and devastating trunk diseases to peach production; however, its pathogenesis is largely unknown. Our previous study showed that L. theobromae LtGPX3, which encodes a glutathione peroxidase resembling yeast GPX3/HYR1-like, was constantly and dramatically upregulated at the infectious stages. Here, we functionally characterized LtGPX3 using the CRISPR-Cas9-aided split marker approach. The ΔLtgpx3 deletion mutants displayed increased sensitivity to the osmotic stress agent KCl and less sensitivity to the cell wall-damaging agent calcofluor white. Exogenous oxidants highly induced the expression of LtGPX3, and the ΔLtgpx3 mutants displayed increased sensitivity to ROS-generating oxidants. Pathogenicity assays revealed that ΔLtgpx3 mutants showed compromised virulence in peach shoots, which was partially restored when peach shoots were pretreated with an NADPH oxidase inhibitor before inoculation. Moreover, ROS levels were strongly boosted, and transcripts of plant defense-related genes were highly induced in the ΔLtgpx3 mutants-infected peach shoots compared with the wild-type-inoculated. Overall, our results showed the essential roles of LtGPX3 in the oxidative stress response and tolerance and pathological functions in L. theobromae. These findings deepen our understanding of the survival strategies of the woody plant-degrading pathogen L. theobromae and provide new insights into developing new strategies for peach gummosis disease control. [ABSTRACT FROM AUTHOR]

Summary: Although maturity date (MD) is an essential factor affecting fresh fruit marketing and has a pleiotropic effect on fruit taste qualities, the underlying mechanisms remain largely unclear. In this study, we functionally characterized two adjacent NAM‐ATAF1/2‐CUC2 (NAC) transcription factors (TFs), PpNAC1 and PpNAC5, both of which were associated with fruit MD in peach.PpNAC1 and PpNAC5 were found capable of activating transcription of genes associated with cell elongation, cell wall degradation and ethylene biosynthesis, suggesting their regulatory roles in fruit enlargement and ripening.Furthermore, PpNAC1 and PpNAC5 had pleiotropic effects on fruit taste due to their ability to activate transcription of genes for sugar accumulation and organic acid degradation.Interestingly, both PpNAC1 and PpNAC5 orthologues were found in fruit‐producing angiosperms and adjacently arranged in all 91 tested dicots but absent in fruitless gymnosperms, suggesting their important roles in fruit development. Our results provide insight into the regulatory roles of NAC TFs in MD and fruit taste. [ABSTRACT FROM AUTHOR]

Summer pruning is an important horticultural operation that is performed to manipulate tree response so as to make it better manageable without a reduction in yield and fruit quality. But the timing is also an important factor in performing this event. Keeping in view the above facts, the present investigation was carried out to elucidate the 'differential response of summer pruning timing on cropping behavior in low-chill peach cvs. Florida Prince and Shan-i-Punjab'. The experiment was carried out at the experimental orchard of the Department of Fruit Science, College of Horticulture and Forestry, Neri, Hamirpur (HP) during the years 2018–2019 and 2019–2020. There are six treatments in the experiment, each with four replications. T1: no pruning; T2: 25% summer pruning on July 10th + winter pruning (WP); T3: 25% summer pruning on July 20th +WP; T4: 25% summer pruning on July 30th +WP; T5: 25% summer pruning on August 10th +WP); and T6: standard winter pruning (SWP). According to the study's findings, combining 25% summer pruning on July 10th with winter pruning resulted in the greatest improvement in terms of vegetative, flowering or blooming, and qualitative parameters, namely fruit weight, fruit shape index, total soluble solid, and sugar content. However, Florida Prince showed positive enhancement of various characteristics and performed better than Shan-i-Punjab. [ABSTRACT FROM AUTHOR]

Organic matter (OM) amendments are often encouraged in sustainable agriculture programs but can create heterogeneous soil environments when applied to perennial crops such as peaches (Prunus persica (L.) Batsch). To better understand the responses of peach roots to non-uniform soil conditions, transcriptomic analysis was performed in a split-root study using uniform soil (the same soil type for all roots) or non-uniform soil (different soil types for each half of the root system) from either (1) autoclaved sand (S), (2) autoclaved sand with autoclaved compost (A), or (3) autoclaved sand with compost which included inherent biological soil life (B). Each uniform soil type (S, A, and B) was grouped and compared by uniform and non-uniform soil comparisons for a total of nine treatments. Comparisons revealed peach roots had differentially expressed genes (DEGs) and gene ontology terms between soil groups, with the S and B groups having a range of 106–411 DEGs and the A group having a range of 19–94 DEGs. Additionally, six modules were identified and correlated (p > 0.69) for six of the nine treatment combinations. This study broadly highlights the complexity of how OM and biological life in the rhizosphere interact with immediate and distant roots and sheds light on how non-homogenous soil conditions can influence peach root gene expression. [ABSTRACT FROM AUTHOR]

The concomitant evaluation of physicochemical properties and development of important diseases, such as brown rot in peaches postharvest, is hampered by the use of destructive methods for physicochemical analysis. Therefore, this study aimed to evaluate the physicochemical characteristics in wounded and unwounded 'Chimarrita' and 'Maciel' peaches inoculated with M. fructicola assessed by non-destructive methods. The fruits were stored at room temperature (25 ºC) for 10 days after harvest. Colorimeter, near-infrared spectroscopy and portable spectrophotometer DA-meter® (single index of absorbance difference, IAD index) were used to evaluate the physicochemical changes that naturally occurred in peaches during postharvest and due to pathogen development on the same fruit simultaneously. Incidence and severity of brown rot were evaluated by counting the number of lesions resulting from inoculation at 24, 48 and 60 h post inoculation. Also, the sporulation of M. fructicola on fruit surface was evaluated. The results indicated that differences between wounded and unwounded fruits related soluble solids content, pulp firmness, titratable acidity, dry matter and IAD did not affect the incidence of M. fructicola in both cultivars. However, the interaction between dry matter and colour at the time of inoculation influenced colonisation and consequent lesion size in inoculated wounded 'Chimarrita' fruits. [ABSTRACT FROM AUTHOR]

Class III peroxidases are plant-specific and play a key role in the response to biotic and abiotic stresses, as well as in plant growth and development. In this study, we investigated 60 POD genes from Prunus persica based on genomic and transcriptomic data available in NCBI and analysed the expression of individual genes with qPCR. Peroxidase genes were clustered into five subgroups using the phylogenetic analysis. Their exon–intron structure and conserved motifs were analysed. Analysis of the transcriptomic data showed that the expression of PpPOD genes varied significantly in different tissues, at different developmental stages and under different stress treatments. All genes were divided into low- and high-expressed genes, and the most highly expressed genes were identified for individual tissues (PpPOD12 and PpPOD42 in flower buds and PpPOD73, PpPOD12, PpPOD42, and PpPOD31 in fruits). The relationship between cold tolerance and the level of peroxidase expression was revealed. These studies were carried out for the first time in the peach and confirmed that chilling tolerance may be related to the specificity of antioxidant complex gene expression. [ABSTRACT FROM AUTHOR]

Cultivation of stone fruit trees in many producing countries is traditionally carried out with low planting density and training in open plant systems (pots). However, the higher density systems and training with more compact mills, for example the 'leading system', have been providing better production. The objective of this work was to evaluate the phenological, vegetative, and productive characteristics of the peach cultivar Della Nona, subjected to various training systems. The work was conducted during two production cycles where three driving systems were evaluated: 'central leader' (5.0 m x 0.8 m, 2,500 plants ha-1), 'Y shape' (5.0 m x 1.5 m, 1,333 plants ha-1) and 'vase' (5.0 m x 3.5 m, 571 plants ha-1). We showed that phenological development was not influenced by the different training systems. The training system 'central leader' gave lower cup size, lower production, but higher productivity. We conclude that the 'central leader' training system adapts to the conditions of the region and shows high productivity for the peach cultivar Della Nona.

In this study, the objective was to ascertain the optimal extraction method for the recovery of polyphenols from two peach cultivars, namely ‘Andross’ and ‘Everts’, at unripe and ripe stages. Two extraction techniques were explored: conventional extraction and cloud-point extraction (CPE), utilizing various solvents, including water, ethanol, 60% ethanol, and the surfactant Tween 80. Moreover, the conditions of CPE (such as pH, ionic strength, surfactant concentration, etc.) were optimized. To elucidate the antioxidant activity of the extracts, the total polyphenol content (TPC), the ferric-reducing antioxidant power (FRAP) assay, and the DPPH antiradical scavenging were measured. Our findings indicate that CPE is a superior method for polyphenol recovery. Unripe fruits had more antioxidants than ripe ones. Unripe ‘Andross’ fruit has a TPC of 1465.32 mg gallic acid equivalents per kilogram (mg GAE/kg). FRAP and DPPH levels were 7.33 and 5.12 mmol ascorbic acid equivalents (AAE/kg), respectively. With a TPC of 1714.53 mg GAE/kg, the unripe fruit from the ‘Everts’ cultivar has even more antioxidant capacity. Additionally, its FRAP and DPPH values were increased at 8.57 and 6.08 mmol AAE/kg, respectively. These findings underscore the efficacy of CPE as a preferred method for polyphenol extraction while also highlighting the enhanced antioxidant potential of unripe peaches, particularly in the ‘Everts’ cultivar.

Taphrina deformans (Berk/Tul) is a causal agent of peach leaf curl that causes severe deformation of leaves and, in some cases, affects plant fruits and shoots, thereby exerting an adverse effect on tree yield and vitality. It has the potential to cause epidemics. The present study indicated ultrastructural alterations in the mesophyll cells of peach leaves, Fayette cultivar, in response to infection by T. deformans . Pathogen-induced hypertrophy and hyperplasia are associated with the unbalanced growth of the infected cells. Changes in the shape of the mesophyll cells from cylindrically oval to isodiametric were found. A strong destruction of the cell compartments was established in a thin parietal cytoplasm. The degradation of chloroplast membrane structures was significant. The regular shape of the chloroplasts was lost with the appearance of concavities. The middle lamella was expanded and exhibited the presence of T. deformans hyphae provoking those alterations. They contacted the cell wall but did not entirely disrupt it. This study confirms and complements the ultrastructural picture of peach leaf curl in the dynamic plant-host system.

Peach production faces significant pre-harvest challenges, including low moisture, nutrient deficiencies, flower drop, physical damage, and surface discoloration, which can limit yield and fruit quality. To mitigate these issues, the present study hypothesized that foliar applications of silicon and zinc could enhance peach growth, yield, and quality due to their known roles in improving stress tolerance, nutrient uptake, and antioxidant activity. Therefore, this research aimed to identify optimal concentrations of silicon and zinc for quality peach production. Ten-year-old peach trees of uniform size were sprayed with four levels of silicon (0%, 0.1%, 0.2%, and 0.3%) and zinc (0%, 0.25%, 0.50%, and 0.75%) for two consecutive growing seasons, at the berry and pit hardening stages, using a Randomized Complete Block Design (RCBD) with three replications. The averaged data from the two years showed that the pre-harvest foliar application of silicon significantly improved all yield and quality attributes of peaches. The foliar application of silicon at 0.3% notably enhanced fruit growth, yield, and biochemical attributes. Additionally, the highest fruit growth, yield, and quality of peach fruits were observed at the 0.75% zinc concentration. Maximum antioxidant activity, flavonoid content, proline content, and catalase activity were observed in fruits from plants treated with 0.3% silicon, which were statistically on par with 0.2% silicon. However, peroxidase activity was highest at 0.2% silicon. Regarding zinc levels, antioxidant activity, flavonoid content, proline content, and peroxidase activity were highest in fruits treated with 0.75% zinc, while catalase activity was superior when fruits were sprayed with 0.50% zinc. The interaction between silicon and zinc concentrations was found to be non-significant for most parameters, except for titratable acidity, TSS–acid ratio, ascorbic acid content, antioxidant activity, flavonoid content, and peroxidase activity. In conclusion, the foliar application of 0.3% silicon and 0.75% zinc independently enhanced all yield and quality characteristics of peaches. For the agro-climatic conditions of Peshawar, 0.2% silicon and 0.50% zinc are recommended for optimal peach production.

Calcium foliar applications are known to effectively enhance peach quality; however, the optimal implementation strategy regarding fruit developmental stages and cultivars remains unclear. In this study, three different moments of fruit Ca applications in peach and nectarine are tested: Early season, Mid-season, and Late season. For this aim, the 44Ca isotope was used as a tracer, enabling the quantification and location of the Ca derived from the foliar fertilizer. Stone, flesh, and skin 44Ca enrichment was separately analyzed at harvest. The results indicate that Ca absorption in the fruits from external CaCl2 applications was influenced by the timing of the application during fruit development, with Late-season applications proving to be the most effective in increasing the Ca content in the fruit, corresponding with a higher fruit size at the application moment. Nevertheless, no differences in the absorption efficiency were found between the three timings of the application. Furthermore, the Ca from the foliar fertilizer in the fruit predominately remained in the flesh, followed by the skin. The Ca derived from the foliar fertilizer reached the stone in all of the experimental situations, but the Early- and Mid-season applications resulted in the highest amount of Ca derived from the fertilizer in this part of the fruit. Interestingly, the peach exhibited a higher Ca absorption efficiency compared to the nectarine, likely due to the presence of trichomes that retain the foliar fertilizer on the fruit surface. In conclusion, the Ca absorption and distribution in peaches depends on the cultivar and timing of the Ca application.