63 results on '"Pellizzari, G"'
Search Results
2. Interstitial lung disease in patients enrolled in early-phase clinical trials: the ILDE study
- Author
-
Trapani, D., Scalia, R., Giordano, E., Castellano, G., Doi, G., Gaeta, A., Pellizzari, G., Carnevale Schianca, A., Katrini, J., D’Ambrosio, S., Santoro, C., Guidi, L., Valenza, C., Belli, C., Gandini, S., Russo, A., and Curigliano, G.
- Published
- 2024
- Full Text
- View/download PDF
3. AllergoOncology: Opposite outcomes of immune tolerance in allergy and cancer
- Author
-
Jensen‐Jarolim, E., Bax, H. J., Bianchini, R., Crescioli, S., Daniels‐Wells, T. R., Dombrowicz, D., Fiebiger, E., Gould, H. J., Irshad, S., Janda, J., Josephs, D. H., Levi‐Schaffer, F., O′Mahony, L., Pellizzari, G., Penichet, M. L., Redegeld, F., Roth‐Walter, F., Singer, J., Untersmayr, E., Vangelista, L., and Karagiannis, S. N.
- Published
- 2018
- Full Text
- View/download PDF
4. 2293P Protumoral role of neutrophils and neutrophils extracellular traps (NETS) in non-metastatic gastroesophageal and rectal cancers
- Author
-
Catozzi, C., Gervaso, L., Benini, L., Ciardiello, D., Zampino, M.G., Spada, F., Borghesani, M., Valenza, C., Algeri, L., Guidi, L., Pellizzari, G., Fazio, N., Nezi, L., and Cella, C.A.
- Published
- 2023
- Full Text
- View/download PDF
5. 2148P Venous thromboembolism (VTE) in patients with advanced high grade ovarian carcinoma (aHGOC) receiving PARP inhibitors
- Author
-
Gervaso, L., Valenza, C., Mongillo, M., Boldrini, L., Bielo, L. Boscolo, Castellano, G., Guidi, L., Katrini, J., Nicolo, E., Pellizzari, G., Trapani, D., Cella, C.A., Lorenzetti, I.T., Derio, S., La Presa, M.T., Parma, G., Fazio, N., Curigliano, G., Khorana, A.A., and Colombo, N.
- Published
- 2023
- Full Text
- View/download PDF
6. 2041P The prevalence of hematologic adverse events (HAEs) and myelodysplastic syndrome/acute myeloid leukemia (MDS/AML) in patients (pts) with advanced high grade ovarian carcinoma (aHGOC) receiving PARP inhibitor (PARPi), with or without a germline BRCA pathogenic variant
- Author
-
Valenza, C., Nicolo, E., Mongillo, M., Trapani, D., Gigli, F., Boldrini, L., Bielo, L. Boscolo, Castellano, G., Guidi, L., Katrini, J., Pellizzari, G., Villa, J., Lorenzetti, I.T., Derio, S., La Presa, M.T., Parma, G., Curigliano, G., and Colombo, N.
- Published
- 2023
- Full Text
- View/download PDF
7. Aspidiella hartii (Cockerell 1895) (Hemiptera: Diaspididae) on yam (Dioscorea spp.) tubers: a new pest regularly entering the European part of the EPPO region.
- Author
-
Salerno, M., Porcelli, F., Mazzeo, G., Suma, P., Russo, A., Diana, L., and Pellizzari, G.
- Subjects
HEMIPTERA ,DIASPIDIDAE ,YAM diseases & pests ,TUBERS ,DISEASES - Abstract
Copyright of EPPO Bulletin is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2018
- Full Text
- View/download PDF
8. First record and establishment of Tuberocephalus (Trichosiphoniella) tianmushanensis Zang, (Hemiptera Aphididae) on ornamental cherry trees in Italy.
- Author
-
Pellizzari, G. and Frigimelica, G.
- Subjects
- *
CHERRIES , *PRUNUS , *HEMIPTERA , *ORNAMENTAL trees ,APHID host plants - Abstract
The occurrence of the Asiatic aphid Tuberocephalus (Trichosiphoniella) tianmushanensis Zang, (=Tuberocephalus (Trichosiphoniella) higansakurae hainnevilleae Remaudière and Sorin) (Hemiptera Aphididae) in Italy is reported. The species was first detected inside leaf galls of Prunus subhirtella cv. pendula trees growing outdoors at the University Botanical Garden of Padua (Italy). Further investigations demonstrated that the species is present in plant nurseries in the Veneto region. So far this species was considered eradicated in Europe, after its first incursion in France in 1993. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
9. PATHOGENIC FUNGI ON TRACHYCARPUS FORTUNEI PALMS INFESTED BY PAYSANDISIA ARCHON.
- Author
-
Frigimelica, G., Pozzebon, A., Duso, C., and Pellizzari, G.
- Subjects
PALM tree diseases & pests ,LEPIDOPTERA ,BORERS (Insects) ,INSECT pests ,NECROSIS - Abstract
In 2009 the alien palm borer Paysandisia archon (Lepidoptera:Castniidae) was first reported in northern Italy (Veneto region) on Trachycarpus fortunei. A survey was carried out during 2009-2011 to study the pest phenology and damage in the infested area. Infested palms were cut down to verify the infestation level. Several palms with severe decline symptoms had only a moderate mechanical damage by P. archon whereas expanding necrosis of leaf bases, starting from the larval galleries, occurred. Therefore, a study on the necrosis agents possibly linked to larval damage was carried out. Samples of leaf bases with larval galleries and necrosis were collected from infested palms. Mature and young leaf base samples were removed from both naturally and artificially infested palms. From each leaf, both necrotic and healthy tissue fragments were removed, incubated on Difco PDA agar and then the emerging fungal colonies were isolated. The most frequent fungus isolated from the necrotic tissues was Talaromyces cf. erythromellis. This species had higher occurrence on necrotic rather than on healthy tissues. In a pathogenicity test by inoculation of leaf bases collected from healthy palms, T. cf. erythromellis was able to induce large necrotic areas. Fusarium proliferatum was also frequently isolated, but it was not associated with the necrotic areas. [ABSTRACT FROM AUTHOR]
- Published
- 2012
10. Developing combination therapies with biologics in triple-negative breast cancer.
- Author
-
Gaudio G, Martino E, Pellizzari G, Cavallone M, Castellano G, Omar A, Katselashvili L, Trapani D, and Curigliano G
- Abstract
Introduction: Novel compounds have entered the triple-negative breast cancer (TNBC) treatment algorithm, namely immune checkpoints inhibitors (ICIs), PARP inhibitors and antibody-drug conjugates (ADCs). The optimization of treatment efficacy can be enhanced with the use of combination treatments, and the incorporation of novel compounds. In this review, we discuss the combination treatments under development for the treatment of TNBC., Areas Covered: The development of new drugs occurring in recent years has boosted the research for novel combinations to target TNBC heterogeneity and improve outcomes. ICIs, ADCs, tyrosine kinase inhibitors (TKIs), and PARP inhibitors have emerged as leading players in this new landscape, while other compounds like novel intracellular pathways inhibitors or cancer vaccines are drawing more and more interest. The future of TNBC is outlined in combination approaches, and based on new cancer targets, including many chemotherapy-free treatments., Expert Opinion: A large number of TNBC therapies have either proved clinically ineffective or weighted by unacceptable safety profiles. Others, however, have provided promising results and are currently in late-stage clinical trials, while a few have actually changed clinical practice in recent years. As novel, more and more selective drugs come up, combination strategies focusing the concept of synergy are fully warranted for the future.
- Published
- 2024
- Full Text
- View/download PDF
11. Non-Small-Cell Lung Cancers (NSCLCs) Harboring RET Gene Fusion, from Their Discovery to the Advent of New Selective Potent RET Inhibitors: "Shadows and Fogs".
- Author
-
Spitaleri G, Trillo Aliaga P, Attili I, Del Signore E, Corvaja C, Pellizzari G, Katrini J, Passaro A, and de Marinis F
- Abstract
RET fusions are relatively rare in Non-Small-Cell Lung Cancers (NSCLCs), being around 1-2% of all NSCLCs. They share the same clinical features as the other fusion-driven NSCLC patients, as follows: younger age, adenocarcinoma histology, low exposure to tobacco, and high risk of spreading to the brain. Chemotherapy and immunotherapy have a low impact on the prognosis of these patients. Multitargeted RET inhibitors have shown modest activity jeopardized by high toxicity. New potent and selective RET inhibitors (RET-Is) (pralsetinib and selpercatinib) have achieved a higher efficacy minimizing the known toxicities of the multitargeted agents. This review will describe the sensitivity of immune-checkpoint inhibitors (ICIs) in RET fusion + NSCLC patients, as well their experiences with the 'old' multi-targeted RET inhibitors. This review will focus on the advent of new potent and selective RET-Is. We will describe their efficacy as well as the main mechanisms of resistance to them. We will further proceed to deal with the new drugs and strategies proposed to overcome the resistance to RET-Is. In the last section, we will also focus on the safety profile of RET-Is, dealing with the main toxicities as well as the rare but severe adverse events.
- Published
- 2024
- Full Text
- View/download PDF
12. Deleterious alterations in homologous recombination repair genes and efficacy of platinum-based chemotherapy in biliary tract cancers.
- Author
-
Belli C, Boscolo Bielo L, Repetto M, Crimini E, Scalia R, Diana A, Orefice J, Ascione L, Pellizzari G, Fusco N, Barberis M, Daniele B, Guerini-Rocco E, and Curigliano G
- Subjects
- Humans, Male, Female, Retrospective Studies, Middle Aged, Aged, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Antineoplastic Combined Chemotherapy Protocols pharmacology, Platinum therapeutic use, Platinum pharmacology, Biliary Tract Neoplasms drug therapy, Biliary Tract Neoplasms genetics, Biliary Tract Neoplasms pathology, Recombinational DNA Repair genetics, Recombinational DNA Repair drug effects
- Abstract
Background: Platinum-based chemotherapy represents the standard first-line treatment for biliary tract cancers (BTC). Deficits in genes involved in the homologous recombination (HR) and DNA damage response (DDR) may confer higher sensitivity to platinum agents., Methods: We retrospectively included patients affected by BTC from 2 Italian institutions. Inclusion criteria consist of the receipt of platinum-based chemotherapy in the metastatic setting and the availability of comprehensive genomic profiling using next-generation sequencing (NGS). Patients were included in the HRD-like group if demonstrated oncogenic or likely oncogenic alterations in HR-/DDR-genes. Clinical endpoints were compared between the HRD-like group and the non-HRD-like group., Results: Seventy-four patients were included, of whom 25 (33%) in the HRD-like group and 49 (66%) in the non-HRD group. With a median follow-up of 26.04 months (interquartile-range [IQR] 9.41-29.27) in the HRD-like group and of 22.48 months (IQR 16.86-40.53) in the non-HRD group, no PFS difference emerged, with a mPFS of 5.18 months in the HRD-like group compared to 6.04 months in the non-HRD group (hazard ratio [HR], 1.017, 95% CI 0.58-1.78; P = .95). No differences were observed in DCR (64% [95 CI 45%-83%] vs 73% [95 CI 61%-86%]; P = .4), and CBR (45% [95% CI 28%-73%] vs 50% [95% CI, 37%-68%]; P = .9) between the HRD-like group and non-HRD groups, respectively. Median OS did not statistically differ between the HRD-like group and non-HRD group (26.7 vs 18.0 months, respectively; HR, 0.670, 0.33 to 1.37, P = .27)., Conclusion: HR-/DDR-genes, when assessed with regular tumor-only NGS panels, provide limited clinical validity to identify patients with BTC more likely to benefit from platinum-based chemotherapy., (© The Author(s) 2024. Published by Oxford University Press.)
- Published
- 2024
- Full Text
- View/download PDF
13. Cancers of Unknown Primary Origin: Real-World Clinical Outcomes and Genomic Analysis at the European Institute of Oncology.
- Author
-
Boscolo Bielo L, Belli C, Crimini E, Repetto M, Ascione L, Pellizzari G, Santoro C, Fuorivia V, Barberis M, Fusco N, Rocco EG, and Curigliano G
- Subjects
- Humans, Male, Female, Middle Aged, Aged, Retrospective Studies, High-Throughput Nucleotide Sequencing methods, Adult, Aged, 80 and over, Mutation, Europe, Neoplasms, Unknown Primary genetics, Neoplasms, Unknown Primary drug therapy, Neoplasms, Unknown Primary pathology, Genomics methods
- Abstract
Background: Cancer of unknown primary origin (CUP) poses a significant challenge due to poor clinical outcomes and limited treatment options. As such, further definition of clinicopathological factors and genomic profile to better adapt treatment strategies is required., Methods: Medical records were interrogated to retrospectively include CUP with available clinical and genomics data at the European Institute of Oncology. Next-generation sequencing (NGS) included targeted panels. Statistical analyses were conducted with R Software 4.2.2., Results: A total of 44 patients were included. With a median follow-up of 39.46 months (interquartile range [IQR] 35.98-47.41 months), median PFS (mPFS) to first-line regimen was 3.98 months (95% CI 3.22-5.98), with a clinical benefit rate of 26% (95% CI 14%-49%), and disease control rate (DCR) limited to 48.28%. Most patients (26 of 31, 83.87%) received platinum-doublet chemotherapy, with no statistically significant difference between first-line treatment regimens. Median OS (mOS) was 18.8 months (95% CI 12.3-39.9), with a 12-month OS rate of 66% (95% CI 50%-85%). All patients received comprehensive genomic profiling (CGP). For 11 patients, NGS was unsuccessful due to low sample quantity and/or quality. For the remaining, TP53 (n = 16, 48%) and KRAS (n = 10, 30%) represented the most altered (alt) genes. No microsatellite instability was observed (0 of 28), while 6 of 28 (21.43%) tumors carried high TMB (≥10 mutation per megabase). Eight of 33 tumors (24.2%) displayed at least one actionable alteration with potential clinical benefit according to ESCAT. Only 2 of them received targeted therapy matched to genomic alterations, with a combined mPFS of 2.63 months (95% CI 1.84-not evaluable) as third-line regimens. Six patients received anti-PD1/PD-L1 therapy, showing a meaningful mPFS of 13 months (95% CI 2.04-not evaluable)., Conclusion: CUP exhibits poor prognosis with limited benefits from standard treatment regimens. A significant proportion of CUPs carry actionable alterations, underscoring the importance of genomic profiling to gather additional treatment opportunities. In addition, immunotherapy might represent a valuable treatment option for a subset of CUP. Finally, accurate definition of sequencing methods and platforms is crucial to overcome NGS failures., (© The Author(s) 2024. Published by Oxford University Press.)
- Published
- 2024
- Full Text
- View/download PDF
14. Beyond PD(L)-1 Blockade in Microsatellite-Instable Cancers: Current Landscape of Immune Co-Inhibitory Receptor Targeting.
- Author
-
Crimini E, Boscolo Bielo L, Berton Giachetti PPM, Pellizzari G, Antonarelli G, Taurelli Salimbeni B, Repetto M, Belli C, and Curigliano G
- Abstract
High microsatellite instability (MSI-H) derives from genomic hypermutability due to deficient mismatch repair function. Colorectal (CRC) and endometrial cancers (EC) are the tumor types that more often present MSI-H. Anti-PD(L)-1 antibodies have been demonstrated to be agnostically effective in patients with MSI-H cancer, but 50-60% of them do not respond to single-agent treatment, highlighting the necessity of expanding their treatment opportunities. Ipilimumab (anti-CTLA4) is the only immune checkpoint inhibitor (ICI) non-targeting PD(L)-1 that has been approved so far by the FDA for MSI-H cancer, namely, CRC in combination with nivolumab. Anti-TIM3 antibody LY3321367 showed interesting clinical activity in combination with anti-PDL-1 antibody in patients with MSI-H cancer not previously treated with anti-PD(L)-1. In contrast, no clinical evidence is available for anti-LAG3, anti-TIGIT, anti-BTLA, anti-ICOS and anti-IDO1 antibodies in MSI-H cancers, but clinical trials are ongoing. Other immunotherapeutic strategies under study for MSI-H cancers include vaccines, systemic immunomodulators, STING agonists, PKM2 activators, T-cell immunotherapy, LAIR-1 immunosuppression reversal, IL5 superagonists, oncolytic viruses and IL12 partial agonists. In conclusion, several combination therapies of ICIs and novel strategies are emerging and may revolutionize the treatment paradigm of MSI-H patients in the future. A huge effort will be necessary to find reliable immune biomarkers to personalize therapeutical decisions.
- Published
- 2024
- Full Text
- View/download PDF
15. Safety and anti-tumour activity of the IgE antibody MOv18 in patients with advanced solid tumours expressing folate receptor-alpha: a phase I trial.
- Author
-
Spicer J, Basu B, Montes A, Banerji U, Kristeleit R, Miller R, Veal GJ, Corrigan CJ, Till SJ, Figini M, Canevari S, Barton C, Jones P, Mellor S, Carroll S, Selkirk C, Nintos G, Kwatra V, Funingana IG, Doherty G, Gould HJ, Pellizzari G, Nakamura M, Ilieva KM, Khiabany A, Stavraka C, Chauhan J, Gillett C, Pinder S, Bax HJ, Josephs DH, and Karagiannis SN
- Subjects
- Female, Humans, Antibodies, Monoclonal adverse effects, Basophils, Folic Acid, Immunoglobulin E, Ovarian Neoplasms
- Abstract
All antibodies approved for cancer therapy are monoclonal IgGs but the biology of IgE, supported by comparative preclinical data, offers the potential for enhanced effector cell potency. Here we report a Phase I dose escalation trial (NCT02546921) with the primary objective of exploring the safety and tolerability of MOv18 IgE, a chimeric first-in-class IgE antibody, in patients with tumours expressing the relevant antigen, folate receptor-alpha. The trial incorporated skin prick and basophil activation tests (BAT) to select patients at lowest risk of allergic toxicity. Secondary objectives were exploration of anti-tumour activity, recommended Phase II dose, and pharmacokinetics. Dose escalation ranged from 70 μg-12 mg. The most common toxicity of MOv18 IgE is transient urticaria. A single patient experienced anaphylaxis, likely explained by detection of circulating basophils at baseline that could be activated by MOv18 IgE. The BAT assay was used to avoid enrolling further patients with reactive basophils. The safety profile is tolerable and maximum tolerated dose has not been reached, with evidence of anti-tumour activity observed in a patient with ovarian cancer. These results demonstrate the potential of IgE therapy for cancer., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
16. B cell profiles, antibody repertoire and reactivity reveal dysregulated responses with autoimmune features in melanoma.
- Author
-
Crescioli S, Correa I, Ng J, Willsmore ZN, Laddach R, Chenoweth A, Chauhan J, Di Meo A, Stewart A, Kalliolia E, Alberts E, Adams R, Harris RJ, Mele S, Pellizzari G, Black ABM, Bax HJ, Cheung A, Nakamura M, Hoffmann RM, Terranova-Barberio M, Ali N, Batruch I, Soosaipillai A, Prassas I, Ulndreaj A, Chatanaka MK, Nuamah R, Kannambath S, Dhami P, Geh JLC, MacKenzie Ross AD, Healy C, Grigoriadis A, Kipling D, Karagiannis P, Dunn-Walters DK, Diamandis EP, Tsoka S, Spicer J, Lacy KE, Fraternali F, and Karagiannis SN
- Subjects
- Humans, Antibodies, Immunity, Humoral, Autoantigens genetics, Tumor Microenvironment, B-Lymphocytes, Melanoma genetics
- Abstract
B cells are known to contribute to the anti-tumor immune response, especially in immunogenic tumors such as melanoma, yet humoral immunity has not been characterized in these cancers to detail. Here we show comprehensive phenotyping in samples of circulating and tumor-resident B cells as well as serum antibodies in melanoma patients. Memory B cells are enriched in tumors compared to blood in paired samples and feature distinct antibody repertoires, linked to specific isotypes. Tumor-associated B cells undergo clonal expansion, class switch recombination, somatic hypermutation and receptor revision. Compared with blood, tumor-associated B cells produce antibodies with proportionally higher levels of unproductive sequences and distinct complementarity determining region 3 properties. The observed features are signs of affinity maturation and polyreactivity and suggest an active and aberrant autoimmune-like reaction in the tumor microenvironment. Consistent with this, tumor-derived antibodies are polyreactive and characterized by autoantigen recognition. Serum antibodies show reactivity to antigens attributed to autoimmune diseases and cancer, and their levels are higher in patients with active disease compared to post-resection state. Our findings thus reveal B cell lineage dysregulation with distinct antibody repertoire and specificity, alongside clonally-expanded tumor-infiltrating B cells with autoimmune-like features, shaping the humoral immune response in melanoma., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
17. Anti-cancer pro-inflammatory effects of an IgE antibody targeting the melanoma-associated antigen chondroitin sulfate proteoglycan 4.
- Author
-
Chauhan J, Grandits M, Palhares LCGF, Mele S, Nakamura M, López-Abente J, Crescioli S, Laddach R, Romero-Clavijo P, Cheung A, Stavraka C, Chenoweth AM, Sow HS, Chiaruttini G, Gilbert AE, Dodev T, Koers A, Pellizzari G, Ilieva KM, Man F, Ali N, Hobbs C, Lombardi S, Lionarons DA, Gould HJ, Beavil AJ, Geh JLC, MacKenzie Ross AD, Healy C, Calonje E, Downward J, Nestle FO, Tsoka S, Josephs DH, Blower PJ, Karagiannis P, Lacy KE, Spicer J, Karagiannis SN, and Bax HJ
- Subjects
- Humans, Mice, Animals, Antigens, Chondroitin Sulfate Proteoglycans, Antibodies, Monoclonal pharmacology, Immunoglobulin E, Tumor Microenvironment, Proteoglycans metabolism, Melanoma metabolism
- Abstract
Outcomes for half of patients with melanoma remain poor despite standard-of-care checkpoint inhibitor therapies. The prevalence of the melanoma-associated antigen chondroitin sulfate proteoglycan 4 (CSPG4) expression is ~70%, therefore effective immunotherapies directed at CSPG4 could benefit many patients. Since IgE exerts potent immune-activating functions in tissues, we engineer a monoclonal IgE antibody with human constant domains recognizing CSPG4 to target melanoma. CSPG4 IgE binds to human melanomas including metastases, mediates tumoricidal antibody-dependent cellular cytotoxicity and stimulates human IgE Fc-receptor-expressing monocytes towards pro-inflammatory phenotypes. IgE demonstrates anti-tumor activity in human melanoma xenograft models engrafted with human effector cells and is associated with enhanced macrophage infiltration, enriched monocyte and macrophage gene signatures and pro-inflammatory signaling pathways in the tumor microenvironment. IgE prolongs the survival of patient-derived xenograft-bearing mice reconstituted with autologous immune cells. No ex vivo activation of basophils in patient blood is measured in the presence of CSPG4 IgE. Our findings support a promising IgE-based immunotherapy for melanoma., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
18. Resistance to Antibody-Drug Conjugates Targeting HER2 in Breast Cancer: Molecular Landscape and Future Challenges.
- Author
-
Guidi L, Pellizzari G, Tarantino P, Valenza C, and Curigliano G
- Abstract
The treatment of HER2-positive metastatic breast cancer (mBC) with Trastuzumab emtansine (T-DM1) and Trastuzumab deruxtecan (T-DXd), two antibody-drug conjugates (ADCs) targeting HER2, is burdened by progression of disease related to the acquisition of mechanisms of resistance. Resistance to T-DM1 is caused by the decrease of HER2 expression, the alteration of intracellular trafficking, the impairment of lysosome functions, the drug expulsion through efflux pumps and the activation of alternative signal pathways. Instead, the decrease of HER2 expression and SLX4 loss of function mutations represent the first evidences of mechanisms of resistance to T-DXd, according to the results of DAISY trial. Several strategies are under evaluation to overcome resistances to anti-HER2 ADCs and improve clinical outcomes in patients progressing on these agents: combinations with tyrosine kinase inhibitors, statins, immune checkpoint inhibitors and synthetic DNA-damaging agents are emerging as promising approaches. Furthermore, novel anti-HER2 ADCs with innovative structures and mechanisms of action are in development, in the attempt to further improve the activity and tolerability of currently available agents.
- Published
- 2023
- Full Text
- View/download PDF
19. Impalement injuries of the shoulder: a case report with literature review.
- Author
-
Scaglia M, Negri S, Pellizzari G, Amarossi A, Pasquetto D, Samaila EM, Maluta T, Vecchini E, Ricci M, Valentini R, and Magnan B
- Subjects
- Humans, Male, Middle Aged, Shoulder, Foreign Bodies diagnostic imaging, Foreign Bodies surgery, Shoulder Injuries, Thoracic Injuries complications, Thoracic Injuries surgery, Wounds, Penetrating complications, Wounds, Penetrating surgery
- Abstract
The management of penetrating skeletal extremity trauma is a clinical challenge even for experienced surgeons. While the treatment of associated vascular injuries should be prioritized, there is still a lack of evidence regarding the management of foreign bodies in case of bone fractures or neurological injuries. Here we present a case of impalement of the right proximal humerus with a construction steel rod. The 54-year-old man was successfully treated without vascular, neurological, and thoracic sequelae. A review of the current literature about the most appropriate extrication sequences and soft tissue reconstruction following massive foreign body injuries was carried out.
- Published
- 2022
- Full Text
- View/download PDF
20. Immunotherapy using IgE or CAR T cells for cancers expressing the tumor antigen SLC3A2.
- Author
-
Pellizzari G, Martinez O, Crescioli S, Page R, Di Meo A, Mele S, Chiaruttini G, Hoinka J, Batruch I, Prassas I, Grandits M, López-Abente J, Bugallo-Blanco E, Ward M, Bax HJ, French E, Cheung A, Lombardi S, Figini M, Lacy KE, Diamandis EP, Josephs DH, Spicer J, Papa S, and Karagiannis SN
- Subjects
- Animals, Humans, Mice, Fusion Regulatory Protein 1, Heavy Chain immunology, Immunoglobulin E metabolism, Immunotherapy methods, Receptors, Chimeric Antigen immunology
- Abstract
Background: Cancer immunotherapy with monoclonal antibodies and chimeric antigen receptor (CAR) T cell therapies can benefit from selection of new targets with high levels of tumor specificity and from early assessments of efficacy and safety to derisk potential therapies., Methods: Employing mass spectrometry, bioinformatics, immuno-mass spectrometry and CRISPR/Cas9 we identified the target of the tumor-specific SF-25 antibody. We engineered IgE and CAR T cell immunotherapies derived from the SF-25 clone and evaluated potential for cancer therapy., Results: We identified the target of the SF-25 clone as the tumor-associated antigen SLC3A2, a cell surface protein with key roles in cancer metabolism. We generated IgE monoclonal antibody, and CAR T cell immunotherapies each recognizing SLC3A2. In concordance with preclinical and, more recently, clinical findings with the first-in-class IgE antibody MOv18 (recognizing the tumor-associated antigen Folate Receptor alpha), SF-25 IgE potentiated Fc-mediated effector functions against cancer cells in vitro and restricted human tumor xenograft growth in mice engrafted with human effector cells. The antibody did not trigger basophil activation in cancer patient blood ex vivo, suggesting failure to induce type I hypersensitivity, and supporting safe therapeutic administration. SLC3A2-specific CAR T cells demonstrated cytotoxicity against tumor cells, stimulated interferon-γ and interleukin-2 production in vitro. In vivo SLC3A2-specific CAR T cells significantly increased overall survival and reduced growth of subcutaneous PC3-LN3-luciferase xenografts. No weight loss, manifestations of cytokine release syndrome or graft-versus-host disease, were detected., Conclusions: These findings identify efficacious and potentially safe tumor-targeting of SLC3A2 with novel immune-activating antibody and genetically modified cell therapies., Competing Interests: Competing interests: SNK and JS are founders and shareholders of Epsilogen Ltd., and HJB is now employed through a fund provided by Epsilogen Ltd., (© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ.)
- Published
- 2021
- Full Text
- View/download PDF
21. Epinephrine drives human M2a allergic macrophages to a regulatory phenotype reducing mast cell degranulation in vitro.
- Author
-
Gotovina J, Bianchini R, Fazekas-Singer J, Herrmann I, Pellizzari G, Haidl ID, Hufnagl K, Karagiannis SN, Marshall JS, and Jensen-Jarolim E
- Subjects
- Epinephrine, Humans, Macrophages, Phenotype, Cell Degranulation, Mast Cells
- Published
- 2020
- Full Text
- View/download PDF
22. Basophil activation test in cancer patient blood evaluating potential hypersensitivity to an anti-tumor IgE therapeutic candidate.
- Author
-
Bax HJ, Khiabany A, Stavraka C, Pellizzari G, Chan Wah Hak C, Robinson A, Ilieva KM, Woodman N, Naceur-Lombardelli C, Gillett C, Pinder S, Gould HJ, Corrigan CJ, Till SJ, Katugampola S, Barton C, Winship A, Ghosh S, Montes A, Josephs DH, Spicer JF, and Karagiannis SN
- Subjects
- Basophil Degranulation Test, Basophils, Humans, Immunoglobulin E, Tetraspanin 30, Hypersensitivity, Neoplasms therapy
- Published
- 2020
- Full Text
- View/download PDF
23. In vivo studies on antibiotic combination for the treatment of carbapenem-resistant Gram-negative bacteria: a systematic review and meta-analysis protocol.
- Author
-
Righi E, Scudeller L, Chiamenti M, Abdelraouf K, Lodise T, Carrara E, Savoldi A, Menghin D, Pellizzari G, Ellis S, Franceschi F, Piddock L, Rebuffi C, Sanguinetti M, and Tacconelli E
- Abstract
Objective: There is poor evidence to determine the superiority of combination regimens versus monotherapy against infections due to carbapenem-resistant (CR) Gram-negative bacteria. In vivo models can simulate the pathophysiology of infections in humans and assess antibiotic efficacy. We aim to investigate in vivo effects of antibiotic combination on mortality and disease burden for infections due to CR Acinetobacter baumannii , Pseudomonas aeruginosa and Enterobacteriaceae and provide an unbiased overview of existing knowledge. The results of the study can help prioritising future research on the most promising therapies against CR bacteria., Methods and Analysis: This protocol was formulated using the Systematic Review Protocol for Animal Intervention Studies (SYRCLE) Checklist. Publications will be collected from PubMed, Scopus, Embase and Web of Science. Quality checklists adapted by Collaborative Approach to Meta-Analysis and Review of Animal Data from Experimental Studies and SYRCLE's risk of bias tool will be used. If the meta-analysis seems feasible, the ES and the 95% CI will be analysed. The heterogeneity between studies will be assessed by I
2 test. Subgroup meta-analysis will be performed when possible to assess the impact of the studies on efficacy of the treatments. Funnel plotting will be used to evaluate the risk of publication bias., Dissemination: This systematic review and meta-analysis is part of a wider research collaboration project, the COmbination tHErapy to treat sepsis due to carbapenem-Resistant bacteria in adult and paediatric population: EvideNCE and common practice (COHERENCE) study that includes also the analyses of in vitro and human studies. Data will be presented at international conferences and the results will be published in peer-reviewed journals., Prospero Registration Number: CRD42019128104(available at: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42019128104)., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY. Published by BMJ.)- Published
- 2020
- Full Text
- View/download PDF
24. Basophils from Cancer Patients Respond to Immune Stimuli and Predict Clinical Outcome.
- Author
-
Bax HJ, Chauhan J, Stavraka C, Khiabany A, Nakamura M, Pellizzari G, Ilieva KM, Lombardi S, Gould HJ, Corrigan CJ, Till SJ, Katugampola S, Jones PS, Barton C, Winship A, Ghosh S, Montes A, Josephs DH, Spicer JF, and Karagiannis SN
- Subjects
- Basophils immunology, Biomarkers, Tumor immunology, Biomarkers, Tumor metabolism, Female, Flow Cytometry, Humans, Immunoglobulin E metabolism, Immunophenotyping, Ovarian Neoplasms immunology, Tetraspanin 30 metabolism, Basophils metabolism, Ovarian Neoplasms metabolism
- Abstract
Basophils are involved in manifestations of hypersensitivity, however, the current understanding of their propensity for activation and their prognostic value in cancer patients remains unclear. As in healthy and atopic individuals, basophil populations were identified in blood from ovarian cancer patients ( n = 53) with diverse tumor histologies and treatment histories. Ex vivo basophil activation was measured by CD63 expression using the basophil activation test (BAT). Irrespective of prior treatment, basophils could be activated by stimulation with IgE- (anti-FcεRI and anti-IgE) and non-IgE (fMLP) mediated triggers. Basophil activation was detected by ex vivo exposure to paclitaxel, but not to other anti-cancer therapies, in agreement with a clinical history of systemic hypersensitivity reactions to paclitaxel. Protein and gene expression analyses support the presence of basophils (CCR3, CD123, FcεRI) and activated basophils (CD63, CD203c, tryptase) in ovarian tumors. Greater numbers of circulating basophils, cells with greater capacity for ex vivo stimulation ( n = 35), and gene signatures indicating the presence of activated basophils in tumors ( n = 439) were each associated with improved survival in ovarian cancer. Circulating basophils in cancer patients respond to IgE- and non-IgE-mediated signals and could help identify hypersensitivity to therapeutic agents. Activated circulating and tumor-infiltrating basophils may be potential biomarkers in oncology.
- Published
- 2020
- Full Text
- View/download PDF
25. Harnessing Therapeutic IgE Antibodies to Re-educate Macrophages against Cancer.
- Author
-
Pellizzari G, Bax HJ, Josephs DH, Gotovina J, Jensen-Jarolim E, Spicer JF, and Karagiannis SN
- Subjects
- Animals, Humans, Receptors, Fc immunology, Antigens, Neoplasm immunology, Immunoglobulin E immunology, Macrophages immunology, Neoplasms immunology
- Abstract
Currently, IgG is the only class of antibodies employed for cancer therapy. However, harnessing the unique biological properties of a different class ( e.g., IgE) could engender potent effector cell activation, and unleash previously untapped immune mechanisms against cancer. IgE antibodies are best known for pathogenic roles in allergic diseases and for protective effector functions against parasitic infestation, often mediated by IgE Fc receptor-expressing macrophages. Notably, IgE possess a very high affinity for cognate Fc receptors expressed by tumor-associated macrophages (TAMs). This paper reviews pre-clinical studies, which indicate control of cancer growth by tumor antigen-specific IgE that recruit and re-educate TAMs towards activated profiles. The clinical development harnessing the antitumor potential of recombinant IgE antibodies in cancer patients is also discussed., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
26. In vivo safety profile of a CSPG4-directed IgE antibody in an immunocompetent rat model.
- Author
-
Williams IP, Crescioli S, Sow HS, Bax HJ, Hobbs C, Ilieva KM, French E, Pellizzari G, Cox V, Josephs DH, Spicer JF, Karagiannis SN, and Mele S
- Subjects
- Animals, Antibody-Dependent Cell Cytotoxicity, Antineoplastic Agents, Immunological adverse effects, Cell Line, Tumor, Cross Reactions, Female, Humans, Immunization, Secondary, Immunocompetence, Immunoglobulin E adverse effects, Mice, Rats, Recombinant Fusion Proteins adverse effects, Antigens, Neoplasm immunology, Antineoplastic Agents, Immunological administration & dosage, Chondroitin Sulfate Proteoglycans immunology, Immunoglobulin E administration & dosage, Membrane Proteins immunology, Recombinant Fusion Proteins administration & dosage
- Abstract
IgE monoclonal antibodies hold great potential for cancer therapy. Preclinical in vivo systems, particularly those in which the antibody recognizes the host species target antigen and binds to cognate Fc receptors, are often the closest approximation to human exposure and represent a key challenge for evaluating the safety of antibody-based therapies. We sought to develop an immunocompetent rat system to assess the safety of a rodent anti-tumor IgE, as a surrogate for the human therapeutic candidate. We generated a rat IgE against the human tumor-associated antigen chondroitin sulfate proteoglycan 4 (CSPG4) and cross-reactive for the rat antigen. We analyzed CSPG4 distribution in normal rat and human tissues and investigated the in vivo safety of the antibody by monitoring clinical signs and molecular biomarkers after systemic administration to immunocompetent rats. Human and rat CSPG4 expression in normal tissues were comparable. Animals receiving antibody exhibited transient mild to moderate adverse events accompanied by mild elevation of serum tryptase, but not of angiotensin II or cytokines implicated in allergic reactions or cytokine storm. In the long term, repeated antibody administration was well tolerated, with no changes in animal body weight, liver and kidney functions or blood cell counts. This model provides preclinical support for the safety profiling of IgE therapeutic antibodies. Due to the comparable antigen tissue distribution in human and rat, this model may also comprise an appropriate tool for proof-of-concept safety evaluations of different treatment approaches targeting CSPG4.
- Published
- 2020
- Full Text
- View/download PDF
27. IgE re-programs alternatively-activated human macrophages towards pro-inflammatory anti-tumoural states.
- Author
-
Pellizzari G, Hoskin C, Crescioli S, Mele S, Gotovina J, Chiaruttini G, Bianchini R, Ilieva K, Bax HJ, Papa S, Lacy KE, Jensen-Jarolim E, Tsoka S, Josephs DH, Spicer JF, and Karagiannis SN
- Subjects
- Antigens, Neoplasm immunology, Biomarkers, Cytokines metabolism, Cytotoxicity, Immunologic, Gene Expression, Humans, Models, Biological, Monocytes immunology, Monocytes metabolism, Neoplasms mortality, Neoplasms pathology, Phagocytosis genetics, Phagocytosis immunology, Prognosis, Protein Binding immunology, Receptors, IgE metabolism, Signal Transduction, Immunoglobulin E immunology, Inflammation Mediators metabolism, Macrophage Activation immunology, Macrophages immunology, Macrophages metabolism, Neoplasms immunology, Neoplasms metabolism
- Abstract
Background: Antibody Fc-driven engagement of macrophages is critical for evoking cellular activation and effector functions and influencing tumour-associated macrophage (TAM) recruitment. We previously reported that IgE class antibodies promote restriction of cancer growth in rodent models associated with significant TAM infiltration. However, the human macrophage-associated IgE-Fc Receptor (FcεR) axis remains unexplored. We investigated the effects of anti-tumour IgE stimulation on human macrophage activation., Methods: Human blood monocyte-differentiated quiescent (M0), classically-(M1) and alternatively-(M2) activated macrophages were crosslinked with IgE and polyclonal antibodies to mimic immune complex formation. We examined surface marker expression, cytokine secretion, protein kinase phosphorylation and gene expression in IgE-stimulated macrophages and IgE antibody-dependent macrophage-mediated cytotoxicity (ADCC) against tumour cells., Findings: A proportion (40%) of M2 and (<20%) M0 and M1 macrophages expressed the high-affinity IgE receptor FcεRI. IgE crosslinking triggered upregulation of co-stimulatory CD80, increased TNFα, IFNγ, IL-1β, IL-12, IL-10, IL-13, CXCL9, CXCL11 and RANTES secretion by M0 and M2 and additionally enhanced MCP-1 by M2 macrophages. IgE-stimulated M1 macrophages retained secretion of pro-inflammatory cytokines. IgE crosslinking enhanced the FcεRI-dependent signalling pathway, including phosphorylation of the Lyn kinase, ERK1/2 and p38 in M2 macrophages and upregulated Lyn gene expression by M1 and M2 macrophages. Anti-tumour IgE engendered ADCC of cancer cells by all macrophage subsets., Interpretation: IgE can engage and re-educate alternatively-activated macrophages towards pro-inflammatory phenotypes and prime all subsets to mediate anti-tumour functions. This points to IgE-mediated cascades with potential to activate immune stroma and may be significant in the clinical development of strategies targeting tumour-resident macrophages., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
28. Combining Immune Checkpoint Inhibitors: Established and Emerging Targets and Strategies to Improve Outcomes in Melanoma.
- Author
-
Khair DO, Bax HJ, Mele S, Crescioli S, Pellizzari G, Khiabany A, Nakamura M, Harris RJ, French E, Hoffmann RM, Williams IP, Cheung A, Thair B, Beales CT, Touizer E, Signell AW, Tasnova NL, Spicer JF, Josephs DH, Geh JL, MacKenzie Ross A, Healy C, Papa S, Lacy KE, and Karagiannis SN
- Subjects
- Animals, Antibodies, Monoclonal pharmacology, Antibodies, Monoclonal therapeutic use, B7-H1 Antigen immunology, CTLA-4 Antigen immunology, Humans, Immunotherapy methods, Antibodies, Monoclonal immunology, Immune System drug effects, Immune System immunology, Melanoma immunology, Melanoma therapy
- Abstract
The immune system employs several checkpoint pathways to regulate responses, maintain homeostasis and prevent self-reactivity and autoimmunity. Tumor cells can hijack these protective mechanisms to enable immune escape, cancer survival and proliferation. Blocking antibodies, designed to interfere with checkpoint molecules CTLA-4 and PD-1/PD-L1 and counteract these immune suppressive mechanisms, have shown significant success in promoting immune responses against cancer and can result in tumor regression in many patients. While inhibitors to CTLA-4 and the PD-1/PD-L1 axis are well-established for the clinical management of melanoma, many patients do not respond or develop resistance to these interventions. Concerted efforts have focused on combinations of approved therapies aiming to further augment positive outcomes and survival. While CTLA-4 and PD-1 are the most-extensively researched targets, results from pre-clinical studies and clinical trials indicate that novel agents, specific for checkpoints such as A2AR, LAG-3, IDO and others, may further contribute to the improvement of patient outcomes, most likely in combinations with anti-CTLA-4 or anti-PD-1 blockade. This review discusses the rationale for, and results to date of, the development of inhibitory immune checkpoint blockade combination therapies in melanoma. The clinical potential of new pipeline therapeutics, and possible future therapy design and directions that hold promise to significantly improve clinical prognosis compared with monotherapy, are discussed.
- Published
- 2019
- Full Text
- View/download PDF
29. Anti-Folate Receptor Alpha-Directed Antibody Therapies Restrict the Growth of Triple-negative Breast Cancer.
- Author
-
Cheung A, Opzoomer J, Ilieva KM, Gazinska P, Hoffmann RM, Mirza H, Marlow R, Francesch-Domenech E, Fittall M, Dominguez Rodriguez D, Clifford A, Badder L, Patel N, Mele S, Pellizzari G, Bax HJ, Crescioli S, Petranyi G, Larcombe-Young D, Josephs DH, Canevari S, Figini M, Pinder S, Nestle FO, Gillett C, Spicer JF, Grigoriadis A, Tutt ANJ, and Karagiannis SN
- Subjects
- Animals, Antineoplastic Agents, Immunological administration & dosage, Antineoplastic Agents, Immunological adverse effects, Cell Line, Tumor, Cell Proliferation, Cell Survival genetics, Disease Models, Animal, Female, Folate Receptor 1 genetics, Folate Receptor 1 metabolism, Gene Expression, Humans, Immunohistochemistry, Mice, Models, Biological, Molecular Targeted Therapy, Neoplasms, Basal Cell, RNA Interference, Signal Transduction, Triple Negative Breast Neoplasms pathology, Tumor Burden, Xenograft Model Antitumor Assays, Antineoplastic Agents, Immunological therapeutic use, Folate Receptor 1 antagonists & inhibitors, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms metabolism
- Abstract
Purpose: Highly aggressive triple-negative breast cancers (TNBCs) lack validated therapeutic targets and have high risk of metastatic disease. Folate receptor alpha (FRα) is a central mediator of cell growth regulation that could serve as an important target for cancer therapy. Experimental Design: We evaluated FRα expression in breast cancers by genomic ( n = 3,414) and IHC ( n = 323) analyses and its association with clinical parameters and outcomes. We measured the functional contributions of FRα in TNBC biology by RNA interference and the antitumor functions of an antibody recognizing FRα (MOv18-IgG1), in vitro , and in human TNBC xenograft models. Results: FRα is overexpressed in significant proportions of aggressive basal like/TNBC tumors, and in postneoadjuvant chemotherapy-residual disease associated with a high risk of relapse. Expression is associated with worse overall survival. TNBCs show dysregulated expression of thymidylate synthase, folate hydrolase 1, and methylenetetrahydrofolate reductase, involved in folate metabolism. RNA interference to deplete FRα decreased Src and ERK signaling and resulted in reduction of cell growth. An anti-FRα antibody (MOv18-IgG1) conjugated with a Src inhibitor significantly restricted TNBC xenograft growth. Moreover, MOv18-IgG1 triggered immune-dependent cancer cell death in vitro by human volunteer and breast cancer patient immune cells, and significantly restricted orthotopic and patient-derived xenograft growth. Conclusions: FRα is overexpressed in high-grade TNBC and postchemotherapy residual tumors. It participates in cancer cell signaling and presents a promising target for therapeutic strategies such as ADCs, or passive immunotherapy priming Fc-mediated antitumor immune cell responses. Clin Cancer Res; 24(20); 5098-111. ©2018 AACR ., (©2018 American Association for Cancer Research.)
- Published
- 2018
- Full Text
- View/download PDF
30. Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology.
- Author
-
Crescioli S, Chiaruttini G, Mele S, Ilieva KM, Pellizzari G, Spencer DIR, Gardner RA, Lacy KE, Spicer JF, Tutt ANJ, Wagner GK, and Karagiannis SN
- Subjects
- Allergens immunology, Antibodies immunology, Antigens, Neoplasm immunology, Cell Line, Cell Line, Tumor, HEK293 Cells, Humans, Hypersensitivity immunology, Immunotherapy methods, Membrane Proteins immunology, Immunoglobulin E immunology, Neoplasms immunology, Neoplasms therapy, Recombinant Proteins immunology
- Published
- 2018
- Full Text
- View/download PDF
31. BRAF inhibitors: resistance and the promise of combination treatments for melanoma.
- Author
-
Griffin M, Scotto D, Josephs DH, Mele S, Crescioli S, Bax HJ, Pellizzari G, Wynne MD, Nakamura M, Hoffmann RM, Ilieva KM, Cheung A, Spicer JF, Papa S, Lacy KE, and Karagiannis SN
- Abstract
Identification of mutations in the gene encoding the serine/threonine-protein kinase, BRAF, and constitutive activation of the mitogen-activated protein kinase (MAPK) pathway in around 50% of malignant melanomas have led to the development and regulatory approval of targeted pathway inhibitor drugs. A proportion of patients are intrinsically resistant to BRAF inhibitors, and most patients who initially respond, acquire resistance within months. In this review, we discuss pathway inhibitors and their mechanisms of resistance, and we focus on numerous efforts to improve clinical benefits through combining agents with disparate modes of action, including combinations with checkpoint inhibitor antibodies. We discuss the merits of combination strategies based on enhancing immune responses or overcoming tumor-associated immune escape mechanisms. Emerging insights into mechanisms of action, resistance pathways and their impact on host-tumor relationships will inform the design of optimal combinations therapies to improve outcomes for patients who currently do not benefit from recent treatment breakthroughs., Competing Interests: CONFLICTS OF INTEREST The authors declare no conflicts of interest.
- Published
- 2017
- Full Text
- View/download PDF
32. Anti-Folate Receptor-α IgE but not IgG Recruits Macrophages to Attack Tumors via TNFα/MCP-1 Signaling.
- Author
-
Josephs DH, Bax HJ, Dodev T, Georgouli M, Nakamura M, Pellizzari G, Saul L, Karagiannis P, Cheung A, Herraiz C, Ilieva KM, Correa I, Fittall M, Crescioli S, Gazinska P, Woodman N, Mele S, Chiaruttini G, Gilbert AE, Koers A, Bracher M, Selkirk C, Lentfer H, Barton C, Lever E, Muirhead G, Tsoka S, Canevari S, Figini M, Montes A, Downes N, Dombrowicz D, Corrigan CJ, Beavil AJ, Nestle FO, Jones PS, Gould HJ, Sanz-Moreno V, Blower PJ, Spicer JF, and Karagiannis SN
- Subjects
- Animals, Cell Line, Tumor, Female, Folate Receptor 1 antagonists & inhibitors, Humans, Ovarian Neoplasms drug therapy, Rats, Rats, Wistar, Signal Transduction, Tumor Necrosis Factor-alpha biosynthesis, Antibodies, Anti-Idiotypic immunology, Folate Receptor 1 immunology, Macrophages immunology, Ovarian Neoplasms immunology, Tumor Necrosis Factor-alpha immunology
- Abstract
IgE antibodies are key mediators of antiparasitic immune responses, but their potential for cancer treatment via antibody-dependent cell-mediated cytotoxicity (ADCC) has been little studied. Recently, tumor antigen-specific IgEs were reported to restrict cancer cell growth by engaging high-affinity Fc receptors on monocytes and macrophages; however, the underlying therapeutic mechanisms were undefined and in vivo proof of concept was limited. Here, an immunocompetent rat model was designed to recapitulate the human IgE-Fcε receptor system for cancer studies. We also generated rat IgE and IgG mAbs specific for the folate receptor (FRα), which is expressed widely on human ovarian tumors, along with a syngeneic rat tumor model expressing human FRα. Compared with IgG, anti-FRα IgE reduced lung metastases. This effect was associated with increased intratumoral infiltration by TNFα
+ and CD80+ macrophages plus elevated TNFα and the macrophage chemoattractant MCP-1 in lung bronchoalveolar lavage fluid. Increased levels of TNFα and MCP-1 correlated with IgE-mediated tumor cytotoxicity by human monocytes and with longer patient survival in clinical specimens of ovarian cancer. Monocytes responded to IgE but not IgG exposure by upregulating TNFα, which in turn induced MCP-1 production by monocytes and tumor cells to promote a monocyte chemotactic response. Conversely, blocking TNFα receptor signaling abrogated induction of MCP-1, implicating it in the antitumor effects of IgE. Overall, these findings show how antitumor IgE reprograms monocytes and macrophages in the tumor microenvironment, encouraging the clinical use of IgE antibody technology to attack cancer beyond the present exclusive reliance on IgG. Cancer Res; 77(5); 1127-41. ©2017 AACR ., (©2017 American Association for Cancer Research.)- Published
- 2017
- Full Text
- View/download PDF
33. Therapeutic targets and new directions for antibodies developed for ovarian cancer.
- Author
-
Bax HJ, Josephs DH, Pellizzari G, Spicer JF, Montes A, and Karagiannis SN
- Subjects
- Female, Humans, Antibodies therapeutic use, Immunization, Passive methods, Ovarian Neoplasms immunology
- Abstract
Antibody therapeutics against different target antigens are widely used in the treatment of different malignancies including ovarian carcinomas, but this disease still requires more effective agents. Improved understanding of the biological features, signaling pathways, and immunological escape mechanisms involved in ovarian cancer has emerged in the past few years. These advances, including an appreciation of the cross-talk between cancer cells and the patient's immune system, have led to the identification of new targets. In turn, potential antibody treatments with various mechanisms of action, including immune activation or toxin-delivery, that are directed at these targets have been developed. Here, we identify established as well as novel targets for antibodies in ovarian cancer, and discuss how they may provide fresh opportunities to identify interventions with enhanced therapeutic potential.
- Published
- 2016
- Full Text
- View/download PDF
34. Targeting folate receptor alpha for cancer treatment.
- Author
-
Cheung A, Bax HJ, Josephs DH, Ilieva KM, Pellizzari G, Opzoomer J, Bloomfield J, Fittall M, Grigoriadis A, Figini M, Canevari S, Spicer JF, Tutt AN, and Karagiannis SN
- Subjects
- Humans, Folate Receptor 1 metabolism, Neoplasms metabolism
- Abstract
Promising targeted treatments and immunotherapy strategies in oncology and advancements in our understanding of molecular pathways that underpin cancer development have reignited interest in the tumor-associated antigen Folate Receptor alpha (FRα). FRα is a glycosylphosphatidylinositol (GPI)-anchored membrane protein. Its overexpression in tumors such as ovarian, breast and lung cancers, low and restricted distribution in normal tissues, alongside emerging insights into tumor-promoting functions and association of expression with patient prognosis, together render FRα an attractive therapeutic target. In this review, we summarize the role of FRα in cancer development, we consider FRα as a potential diagnostic and prognostic tool, and we discuss different targeted treatment approaches with a specific focus on monoclonal antibodies. Renewed attention to FRα may point to novel individualized treatment approaches to improve the clinical management of patient groups that do not adequately benefit from current conventional therapies., Competing Interests: The authors declare that they have no conflicts of interest.
- Published
- 2016
- Full Text
- View/download PDF
35. Investigating Biological Control Agents for Controlling Invasive Populations of the Mealybug Pseudococcus comstocki in France.
- Author
-
Malausa T, Delaunay M, Fleisch A, Groussier-Bout G, Warot S, Crochard D, Guerrieri E, Delvare G, Pellizzari G, Kaydan MB, Al-Khateeb N, Germain JF, Brancaccio L, Le Goff I, Bessac M, Ris N, and Kreiter P
- Subjects
- Animals, Biological Control Agents, DNA Barcoding, Taxonomic, France, Host-Parasite Interactions, Parasites isolation & purification, Parasites physiology, Phylogeny, Population Control, Hemiptera parasitology, Insect Control methods, Parasites classification, Pest Control, Biological methods
- Abstract
Pseudococcus comstocki (Hemiptera: Pseudococcidae) is a mealybug species native to Eastern Asia and present as an invasive pest in northern Italy and southern France since the start of the century. It infests apple and pear trees, grapevines and some ornamental trees. Biocontrol programmes against this pest proved successful in central Asia and North America in the second half of the 20th century. In this study, we investigated possible biocontrol agents against P. comstocki, with the aim of developing a biocontrol programme in France. We carried out systematic DNA-barcoding at each step in the search for a specialist parasitoid. First we characterised the French target populations of P. comstocki. We then identified the parasitoids attacking P. comstocki in France. Finally, we searched for foreign mealybug populations identified a priori as P. comstocki and surveyed their hymenopteran parasitoids. Three mealybug species (P. comstocki, P. viburni and P. cryptus) were identified during the survey, together with at least 16 different parasitoid taxa. We selected candidate biological control agent populations for use against P. comstocki in France, from the species Allotropa burrelli (Hymenoptera: Platygastridae) and Acerophagus malinus (Hymenoptera: Encyrtidae). The coupling of molecular and morphological characterisation for both pests and natural enemies facilitated the programme development and the rejection of unsuitable or generalist parasitoids.
- Published
- 2016
- Full Text
- View/download PDF
36. Are Phenacoccus solani Ferris and P. defectus Ferris (Hemiptera: Pseudococcidae) distinct species?
- Author
-
Chatzidimitriou E, Simonato M, Watson GW, Martinez-Sañudo I, Tanaka H, Zhao J, and Pellizzari G
- Subjects
- Animal Distribution, Animal Structures anatomy & histology, Animal Structures growth & development, Animals, Body Size, Female, Hemiptera anatomy & histology, Hemiptera genetics, Hemiptera growth & development, Insect Proteins genetics, Male, Organ Size, Phylogeny, Hemiptera classification
- Abstract
Among the Nearctic species of Phenacoccus (Hemiptera: Pseudococcidae), Phenacoccus solani Ferris and P. defectus Ferris are morphologically similar and it can be difficult to separate them on the basis of microscopic morphological characters of the adult female alone. In order to resolve their identity, a canonical variates morphological analysis of 199 specimens from different geographical origins and host plants and a molecular analysis of the COI and 28S genes were performed. The morphological analysis supported synonymy of the two species, as although the type specimens of the "species" are widely separated from each other in the canonical variates plot, they are all part of a continuous range of variation. The molecular analysis showed that P. solani and P. defectus are grouped in the same clade. On the basis of the morphological and molecular analyses, P. defectus is synonymized under the senior name P. solani, syn. n.
- Published
- 2016
- Full Text
- View/download PDF
37. First record of the Kuwana pine mealybug Crisicoccus pini (Kuwana) in Italy: a new threat to Italian pine forests?
- Author
-
Boselli M and Pellizzari G
- Subjects
- Animal Distribution, Animal Structures anatomy & histology, Animal Structures growth & development, Animals, Body Size, Female, Forests, Hemiptera anatomy & histology, Hemiptera growth & development, Italy, Organ Size, Hemiptera classification, Pinus parasitology, Plant Diseases parasitology
- Abstract
The Asiatic Kuwana pine mealybug, Crisicoccus pini (Kuwana, 1902) (Hemiptera, Pseudococcidae), is reported in Italy for the first time. It was detected in September 2015 on maritime pine, Pinus pinaster, and stone pine, Pinus pinea, trees growing in the town of Cervia (Ravenna Province), Northern Italy. The mealybug has caused yellowing and decline of the pine trees. Pinus pinea is recorded here as a new host for C. pini.
- Published
- 2016
- Full Text
- View/download PDF
38. Check list and zoogeographic analysis of the scale insect fauna (Hemiptera: Coccomorpha) of Greece.
- Author
-
Pellizzari G, Chadzidimitriou E, Milonas P, Stathas GJ, and Kozár F
- Subjects
- Animals, Checklist, Greece, Hemiptera physiology, Animal Distribution, Hemiptera classification
- Abstract
This paper presents an updated checklist of the Greek scale insect fauna and the results of the first zoogeographic analysis of the Greek scale insect fauna. According to the latest data, the scale insect fauna of the whole Greek territory includes 207 species; of which 187 species are recorded from mainland Greece and the minor islands, whereas only 87 species are known from Crete. The most rich families are the Diaspididae (with 86 species), followed by Coccidae (with 35 species) and Pseudococcidae (with 34 species). In this study the results of a zoogeographic analysis of scale insect fauna from mainland Greece and Crete are also presented. Five species, four from mainland Greece and one from Crete are considered to be endemic. Comparison with the scale insect fauna of other countries is provided.
- Published
- 2015
- Full Text
- View/download PDF
39. Role of oxidative stress mediated by glutathione-s-transferase in thiopurines' toxic effects.
- Author
-
Pelin M, De Iudicibus S, Fusco L, Taboga E, Pellizzari G, Lagatolla C, Martelossi S, Ventura A, Decorti G, and Stocco G
- Subjects
- Azathioprine adverse effects, Azathioprine metabolism, Azathioprine toxicity, Cell Proliferation drug effects, Cells, Cultured, Dose-Response Relationship, Drug, Humans, Purines adverse effects, Purines metabolism, Glutathione Transferase metabolism, Oxidative Stress drug effects, Purines toxicity
- Abstract
Azathioprine (AZA), 6-mercaptopurine (6-MP), and 6-thioguanine (6-TG) are antimetabolite drugs, widely used as immunosuppressants and anticancer agents. Despite their proven efficacy, a high incidence of toxic effects in patients during standard-dose therapy is recorded. The aim of this study is to explain, from a mechanistic point of view, the clinical evidence showing a significant role of glutathione-S-transferase (GST)-M1 genotype on AZA toxicity in inflammatory bowel disease patients. To this aim, the human nontumor IHH and HCEC cell lines were chosen as predictive models of the hepatic and intestinal tissues, respectively. AZA, but not 6-MP and 6-TG, induced a concentration-dependent superoxide anion production that seemed dependent on GSH depletion. N-Acetylcysteine reduced the AZA antiproliferative effect in both cell lines, and GST-M1 overexpression increased both superoxide anion production and cytotoxicity, especially in transfected HCEC cells. In this study, an in vitro model to study thiopurines' metabolism has been set up and helped us to demonstrate, for the first time, a clear role of GST-M1 in modulating AZA cytotoxicity, with a close dependency on superoxide anion production. These results provide the molecular basis to shed light on the clinical evidence suggesting a role of GST-M1 genotype in influencing the toxic effects of AZA treatment.
- Published
- 2015
- Full Text
- View/download PDF
40. Description of female nymphal instars and adult female of Kermes echinatus Balachowsky (Hemiptera, Coccoidea, Kermesidae) based on specimens from Crete and mainland Greece, with a discussion on geographical variation.
- Author
-
Porcelli F and Pellizzari G
- Subjects
- Animal Distribution, Animal Structures anatomy & histology, Animal Structures growth & development, Animals, Body Size, Female, Greece, Hemiptera anatomy & histology, Hemiptera growth & development, Male, Nymph classification, Nymph growth & development, Organ Size, Hemiptera classification, Nymph anatomy & histology
- Abstract
The first-instar nymph, second- and third-instar female nymphs and the adult female of Kermes echinatus Balachowsky (Hemiptera, Coccoidea, Kermesidae) are described and illustrated; micrographs of morphological details are also provided. The species was collected on the island of Crete (Greece) and on mainland Greece, new localities for this species, and are compared with Israeli specimens from where it was originally described.
- Published
- 2014
- Full Text
- View/download PDF
41. Latin abbreviations and words used in scale insect literature (Hemiptera: Sternorrhyncha: Coccoidea).
- Author
-
Williams DJ and Pellizzari G
- Subjects
- Animals, Hemiptera anatomy & histology, Hemiptera classification, Terminology as Topic
- Published
- 2014
- Full Text
- View/download PDF
42. A tiny invasive melanoma: a case report with dermatoscopy and dermatopathology.
- Author
-
Pellizzari G, Magee J, Weedon D, and Rosendahl C
- Abstract
We present a case of an early invasive melanoma (Breslow thickness 0.25 mm), 1.6 mm in diameter on the arm of a 38-year-old woman. She was under surveillance due to having multiple (>100) nevi, and the melanoma was assessed as a new lesion by the examining doctor. Clinically the lesion was hyper-pigmented compared with surrounding nevi and dermatoscopically it had a clue of pseudopods/lines radial, but they were arranged in an arguably symmetrical circumferential pattern around a structureless blue-gray center. Generally melanomas are expected to be dermatoscopically asymmetrical, but we believe that this case illustrates the fact that small melanomas may be recognized by clues such as pseudopods/lines radial and dermatoscopic gray even when they have not yet developed unequivocal asymmetry.
- Published
- 2013
- Full Text
- View/download PDF
43. Simple rules on adjectival endings in zoological nomenclature and their use in scale insect names (Hemiptera: Sternorrhyncha: Coccoidea), with some corrections to combinations in common use.
- Author
-
Pellizzari G and Williams DJ
- Subjects
- Animals, Female, Male, Hemiptera classification, Terminology as Topic
- Abstract
We give some simple rules on Latin grammar to help scale insect systematists to apply the correct adjectival gender endings to species names. We list some common errors that have occurred when species names have either been proposed with a wrong gender ending or when they have been transferred from one genus to another. We also correct the gender endings to several species names in a few families.
- Published
- 2013
- Full Text
- View/download PDF
44. A new species of Poliaspoides MacGillivray (Hemiptera, Diaspididae) on Bambusa siamensis (Poaceae) imported into Turkey.
- Author
-
Ulgenturk S and Pellizzari G
- Subjects
- Animal Structures anatomy & histology, Animal Structures growth & development, Animals, Body Size, Female, Hemiptera anatomy & histology, Hemiptera growth & development, Introduced Species, Organ Size, Turkey, Bambusa parasitology, Hemiptera classification
- Abstract
The adult female and first-instar nymph of a new species of armoured scale insect, Poliaspoides bambusae sp.n. (Diaspididae), is described and illustrated. The new species was collected in Turkey on imported ornamental Bambusa siamensis (Poaceae) from an unknown source.
- Published
- 2013
- Full Text
- View/download PDF
45. Three new species in the subfamily Eriopeltinae Sulc from Italy (Hemiptera, Coccoidea, Coccidae) with comments on the genus Lecanopsis.
- Author
-
Pellizzari G
- Subjects
- Animal Distribution, Animals, Female, Hemiptera physiology, Italy, Species Specificity, Hemiptera anatomy & histology, Hemiptera classification
- Abstract
Three new coccid species, namely Hadzibejliaspis ferenci Pellizzari n. sp., Lecanopsis sicula Pellizzari n. sp. and L. salvatorei Pellizzari n. sp. are described and illustrated. Identification keys for the genera in the subfamily Eriopeltinae Sulc and to species in the genera Hadzibejliaspis Koteja and Lecanopsis Targioni Tozzetti are provided.
- Published
- 2013
46. "Single step" PCR with a sensitivity similar to nested PCR for the detection of hepatitis C virus RNA.
- Author
-
Farma E, Boeri E, Morsica G, McDermott J, Soldini L, Repetto CM, Ferioli B, Pellizzari G, Molinari F, and Molinelli A
- Subjects
- Hepacivirus genetics, Hepatitis C blood, Hepatitis C genetics, Humans, Sensitivity and Specificity, Hepacivirus isolation & purification, Polymerase Chain Reaction methods, RNA, Viral analysis
- Abstract
Objective: Evaluation of the performance of different HCV PCR detection systems for HCV RNA: A nested PCR, considered the reference assay, was compared with two single-step methods (ss-PCR): the first is based on the detection of PCR products by liquid hybridization with a 32P end-labelled probe (isotopic ss-PCR), while the second assay is a colorimetric method (colorimetric ss-PCR) using microwell plate hybridization with a specific nucleic acid probe (Amplicor HCV PCR, Roche Diagnostics Systems)., Methods: Sera from 56 patients with suspected hepatitis C infection based on reactive serology or altered liver parameters, and sera from 15 blood donors were tested for HCV RNA: After RNA extraction, the synthesized HCV cDNA was amplified in parallel using isotopic ss-PCR, colorimetric ss-PCR and nested PCR. The products were detected by autoradiography, color development and ethidium bromide fluorescence, respectively., Results: In order to assess the analytical sensitivity of ss-PCR versus that of nested of PCR, experiments included serial dilutions of positive control samples. Results showed that both methods had an extinction signal at the 1:512 dilution. A comparative analysis of 71 clinical sera samples was obtained using the three protocols and the results clearly documented 100% concordance., Conclusions: Single step PCR methods for HCV RNA have a sensitivity equal to that of nested PCR and appear more suitable for diagnostic applications. Ss-PCR is safer than nested PCR in terms of both specificity and contamination problems. In particular, the Roche Amplicor HCV PCR assay minimizes sample exposure and management problems.
- Published
- 1995
47. [Home care for the diabetic within the limits of project--object: pilot project integrated into the health education of the diabetic. Veneto Region].
- Author
-
Collareda G, Gomitolo O, Pellizzari G, Corradin H, and Erle G
- Subjects
- Activities of Daily Living, Aged, Comorbidity, Diabetes Complications, Geriatric Assessment, Humans, Pilot Projects, Workload, Diabetes Mellitus nursing, Home Care Services organization & administration, Patient Education as Topic organization & administration, Public Health Nursing organization & administration
- Abstract
Twenty one district-nurses working in 13 districts were handed a questionnaire (one questionnaire for each patient), to collect data on nursing interventions and main problems of the 382 diabetic patients cared for. The diabetic patients are a very demanding population with a mean age of 78 years; 314 (82%) have a comorbidity, 272 (71%) severe limits on their physical functioning and 233 (61%) the diabetic foot; 183 patients (47%) are not capable of taking autonomous decisions. The nursing interventions for these patients are both technical (drawing blood samples, diagnostic exams) and educational on how to manage and monitor self-administration of insulin (31 patients, 10%), on self monitoring techniques (116 patients, 30%), on alimentation in general and change in food habits (289 patients, 94.1%). The role of the nurse in promoting patients' independence and in preventing complications is highlighted.
- Published
- 1995
48. Single step immunophenotyping of acute leukemias not classifiable by standard morphology and cytochemistry: a practical approach.
- Author
-
Raimondi R, Pellizzari G, and Rodeghiero F
- Subjects
- Acute Disease, Adolescent, Adult, Aged, Antibody Specificity, Female, Flow Cytometry, Humans, Leukemia diagnosis, Microscopy, Fluorescence, Middle Aged, Neoplastic Stem Cells ultrastructure, Predictive Value of Tests, Pregnancy, Antibodies, Monoclonal immunology, Antigens, CD analysis, Antigens, Neoplasm analysis, Biomarkers, Tumor analysis, Immunophenotyping methods, Leukemia classification, Neoplastic Stem Cells chemistry
- Abstract
Background: Immunophenotyping is at present a useful aid and often an essential step for correct diagnosis of acute leukemia and for this purpose some investigators have proposed several immunomarker panels. This approach is particularly important in the differential diagnosis of acute leukemias not classifiable by standard morphology and cytochemistry., Methods: We have tested peripheral blood and/or bone marrow samples from 125 patients not classifiable by FAB criteria. In all the cases, the reactivities of the same panel of 17 monoclonal antibodies were analyzed by flow cytometry, using both single and double fluorescent labeling., Results: Of the 125 patients investigated, 75 (60%) were classifiable as ALL, 58 as B-lineage ALL and 17 as T-lineage ALL; 33 (26.4%) as AML, of which 2 M7; 6 (4.8%) as biphenotypic and 11 (8.8%) as immunophenotypically undifferentiated., Conclusions: From a critical analysis of our cases and a review of the literature, we suggest that a panel of 9 monoclonal antibodies (CD2, CD5, CD7, CD10, CD19, CD20, CD13, CD33, CD41), is sufficient for reliable, rapid and reasonably low cost typing of acute leukemia, useful for an immediate therapeutic decision.
- Published
- 1993
49. The clinical significance of the antiplatelet antibody test based on results for 265 thrombocytopenic patients.
- Author
-
Tosetto A, Ruggeri M, Schiavotto C, Pellizzari G, and Rodeghiero F
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Antibody Specificity, Child, Evaluation Studies as Topic, Female, Humans, Male, Platelet Count, Predictive Value of Tests, Prednisone therapeutic use, Prospective Studies, Thrombocytopenia blood, Thrombocytopenia classification, Thrombocytopenia drug therapy, Treatment Outcome, Antibodies blood, Blood Platelets immunology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Thrombocytopenia immunology
- Abstract
Background: The usefulness of measuring antiplatelet antibodies by ELISA or cytofluorimetric techniques for the diagnosis of idiopathic thrombocytopenic purpura is still uncertain., Methods: We evaluated the clinical significance of two widely applicable antiplatelet antibody tests for a series of 265 patients evaluated consecutively in our Department for the diagnosis or follow-up of thrombocytopenia. Flow cytofluorimetry was used to measure platelet-associated immunoglobulins (PAIg) and the enzyme-linked immunosorbent assay (ELISA) was used to detect antiplatelet antibodies in patients sera (S-PBIg). The significance of antiplatelet antibody testing was addressed by studying the factors that influence test positivity, the diagnostic value of a positive test and the prognostic implication of a positive test., Results: The platelet count was found to be strongly associated with positive PAIg or S-PBIg (P < 0.001), while neither splenectomy nor corticosteroid treatment affected PAIg or S-PBIg positivity. Both PAIg and S-PBIg were limited diagnostic value for the differential diagnosis of idiopathic thrombocytopenic purpura (ITP) from secondary thrombocytopenia for patients with platelet counts between 25,000 and 100,000 platelets/microliters, but the percentages of misclassified patients based on only the PAIg or S-PBIg test were 32 and 54%. We found no relationship between PAIg and/or S-PBIg at time of diagnosis and the patient's clinical response to corticosteroid therapy., Conclusions: We conclude that antiplatelet antibodies are strongly correlated with platelet counts, discriminate poorly between ITP and secondary thrombocytopenia and have negligible prognostic value. Therefore, we do not recommend performing antiplatelet antibody tests as a routine laboratory test in the diagnostic workup of thrombocytopenia.
- Published
- 1993
50. [Comparative clinical study of a new imidazole molecule (fluconazole) and ketaconazole in the treatment of Candida albicans vulvovaginitis].
- Author
-
Mazziotti F, Cirillo L, Arena V, Cipriani P, Ghezzi C, Bresadola M, Ragni N, Donadio C, and Pellizzari G
- Subjects
- Adult, Drug Evaluation, Female, Fluconazole administration & dosage, Humans, Ketoconazole administration & dosage, Middle Aged, Candidiasis, Vulvovaginal drug therapy, Fluconazole therapeutic use, Ketoconazole therapeutic use
- Abstract
A multicentre trial was carried out in Italy with the aim of comparing the efficacy, safety and tolerability of the oral administration of fluconazole with the oral administration of ketoconazole in the treatment of patients affected by Candida vulvovaginitis. A total of 174 patients with symptomatic Candida vulvovaginitis were identified both by objective examination and cell culture tests: of these 87 were treated using a single oral administration of fluconazole (150 mg) whereas the other 87 received 2 200 mg capsules of ketoconazole daily for 5 days. Tests to assess the efficacy, safety and tolerability of both treatments were carried out approximately 7 days and 5-6 weeks from the start of therapy. The results obtained showed a success rate of 92% for fluconazole-treated patients and 83% for those treated with ketoconazole. In addition to the rapid and safe efficacy of treatment, the most important findings which emerged from this study were the extreme simplicity of use, excellent patient compliance and the complete absence of collateral effects of variations in the hematochemical and urine parameters taken into consideration caused by fluconazole.
- Published
- 1992
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.