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We estimated the concentration of Chlorophyll-a (Chl-a) along with physical and biological factors including sea surface temperature (SST), wind speed and concentration of nitrate and phosphate in the Western, Central and Eastern Equatorial Indian Oceans (WEIO, CEIO and EEIO) using data from the Copernicus Marine Service and ERA-5 for the years 1994 to 2019. Our results show that along with the concentration of Chl-a, nitrate and phosphate have decreased, and sea surface temperature has increased in the EEIO. Due to coastal upwelling off the coast of Somalia and offshore Ekman transport throughout the summer, the southwest monsoon caused a significant increase in concentrations of Chl-a, nitrate, phosphate and wind speed in WEIO during June-July-August-September (JJAS) and October-November-December (OND). SST decreased at this time and deeper water interacted with surface water, increasing biological production. In EEIO, the upwelling zone is primarily maintained by wind-induced divergence throughout the year, whereas stratification in winter prevented surface Chl-a increase. High wind speed and low SST led to increased productivity in WEIO. Comparison of EEIO with WEIO and CEIO demonstrates low levels of Chl-a, phosphate and nitrate. The trends signify that these oceanic zones are highly vulnerable to developing into ecological deserts in the near future. [ABSTRACT FROM AUTHOR]

An equiatomic quinary TiVZrMoW refractory high entropy alloy (RHEA) is selected for studying phase evolution and stability. The prediction of phases that may form on synthesis is made by (i) Semi-empirical /empirical methods (based on extended Hume-Rothery rules), (ii) CALPHAD and (iii) ab-initio methods. Mixing enthalpy (calculated by Miedema method) and valence electron concentration predicted the formation of the BCC phase ( ; and VEC = 5). However, the atomic mismatch factor, δ (7.17%) value being higher than the desired value, can de-stabilize the lattice to form a multi-phase structure. CALPHAD technique predicted that at room temperature three BCC and two Laves phases would form. The enthalpy of mixing (4.25 kJ/mol) calculated using Density Functional Theory (DFT) also suggests phase separation at room temperature. The phases and microstructure that evolved in the as-cast and annealed samples are studied by X-ray diffraction (XRD), scanning electron and transmission electron microscopy (TEM) techniques. Two BCC phases and a minor amount of ordered B2 and C15 type Laves phase are found to co-exist in the as-cast sample. The primary BCC phase (BCC#1) is rich in Mo and W (a = 3.17 ± 0.02 Å), while the minor BCC phase (BCC#2) is rich in Zr and Ti (a = 3.65 ± 0.02 Å). The annealed sample shows the transformation of both the disordered BCC phase to the ordered B2 phases and the increase in the amount of C15 Laves phase. The phase evolution and their relative stability in this RHEA has been discussed after analysing the experimental and theoretical results. [ABSTRACT FROM AUTHOR]

Stable silver nanoparticles of various sizes are synthesised using the aqueous extract of Tulsi (Ocimum tenuiflorum) as the reducing agent as well as a stabilising agent under the influence of light of wavelength ranging from 200 to 900 nm. The formation of silver nanoparticles is monitored using a UV-Visible spectrophotometer and the particle shape, size and stability are analysed using scanning electron microscopy, dynamic light scattering and zeta potential measurement techniques. It is observed that the size of the silver nanoparticles decreases on increasing the wavelength of irradiating light. The silver nanoparticles show antimicrobial activity against gram-positive (Bacillus subtilis), as well as gram-negative (Escherichia coli) bacteria. The antimicrobial activity shows an inverse relationship with the size of the silver nanoparticles. The bio-synthesised silver nanoparticles are successfully embedded in a polymeric film to act as an active antimicrobial film that can potentially release the active constituents. [ABSTRACT FROM AUTHOR]

Stable silver nanoparticles (AgNPs) of size 80 ± 11 nm produced by chitosan (CH) mediated green synthesis were blended with polyvinyl alcohol (PVA) to form electrospun fibrous composite nano-layers (FCNLs). The chitosan acted as the stabilising as well as an antimicrobial agent in combination with the AgNPs which were characterised using UV–visible spectrophotometry, dynamic light scattering (DLS) and scanning electron microscopy (SEM). The crystallinity and chemical nature of the electrospun composite was characterised by using X-ray diffraction (XRD) and FTIR spectroscopy, respectively, and its hydrophobicity was characterised by measuring the water contact angle. The electrospun composite showed effective antimicrobial activity against Listeria monocytogenes (gram +ve) and Escherichia coli (gram –ve) bacterial species. The electrospun composite, when tested as packaging material for meat, showed bio-activity and extended the meat shelf-life by one week. The electrospun nanocomposite is able to inhibit microbial degradation of packaged food and extend its keeping quality in an eco-friendly manner. Unlabelled Image • Chitosan-based fibrous composite nano-layers synthesised for food packaging. • Composite packaging possesses antimicrobial property. • The packaged meat displayed extended shelf-life and better organoleptic quality. • The composite packaging can replace plastics and have low environmental impact. [ABSTRACT FROM AUTHOR]

Microbial infection and oxidative damage of the fibroblast often results in prolonged and incomplete wound healing. Therefore, there is an increasing demand for a scaffold being effective to prevent any possible infection and neutralize excessively released free radicals. Herein, we designed a PCL-based nanofiber loaded with ciprofloxacin hydrochloride (CHL) and quercetin. Developed nanofiber showed the formation of smooth and continuous nanofiber with 101.59 ± 29.18 nm average diameter and entrapping the drugs in amorphous form without any possible physico-chemical interaction between drugs and excipient. High entrapment efficiency (CHL: 92.04% and Que: 94.32%) and prolonged in-vitro release (for 7 days) demonstrated the capability of scaffold to suppress any probable infection and oxidative damage, which was further confirmed by in-vitro antibacterial and antioxidant activity. The biocompatibility of scaffold for direct application to wound site was evaluated through hemocompatibility and cytocompatibility assay. The wound healing efficacies of nanofiber were assessed using full thickness wound model in rats, which displayed accelerated wound healing with complete re-epithelialization and improved collagen deposition within 16 days. In-vivo wound healing finding was further corroborated by SOD, catalase, and hydroxyproline assay. The current study validates the application of ciprofloxacin HCl and quercetin functionalized nanofiber as a potential wound dressing material. [ABSTRACT FROM AUTHOR]

Hyperglycemia associated ER stress has been found as a critical contributor in the pathogenesis of type 2 diabetes mellitus. However, reports regarding molecular mechanisms involved are limited. This study was aimed to identify the role of ER stress in regulating hepatic glucose metabolism and its link with oxidative stress. Further, this study explores the novel role of Morin, a flavonol, in modulating ER stress in STZ/nicotinamide induced type 2 diabetic male Wistar rats. Results demonstrate that hyperglycemia induced ER stress in rats and significantly lowered the expression of glucose transporter proteins resulting in impaired glucose metabolism during diabetes. Morin was found to downregulate PERK-eIF2α-ATF4 pathway by interacting with PERK protein as confirmed through pull-down assay. Additionally, Morin maintained the reducing environment in ER and enhanced PDI activity compared to diabetic rats. Morin prevented cell death by suppressing the expression of PERK dependent pro-apoptotic proteins including ATF4 and CHOP. Findings from this study affirm the role of ER stress in hyperglycemia induced gluco-metabolic aberrations and liver injury as confirmed by ISRIB, a standard chemical ER stress inhibitor. Notably, Morin promoted deactivation of UPR sensors and upregulated PDI activity endorsing its anti-ER stress potential which may allow the development of new therapeutic avenues to target hyperglycemic hepatotoxicity. Unlabelled Image • PERK-eIF2α-ATF4 pathway plays a critical role in the progression of diabetic hepatotoxicity. • Inhibition of PERK-eIF2α-ATF4 pathway enhances gluco-metabolism in diabetic liver. • Morin prevents proteotoxic cell death by selectively down-regulating PERK response pathway. • Morin shows ER stress inhibitory potential that improves glucose metabolism and prevents liver injury during diabetes. [ABSTRACT FROM AUTHOR]

The present paper deals with the properties of a Finsler space F* n whose met- ric is obtained from the metric of another Finsler space Fn defined over the same man- ifold, with the help of a contravariant vector vi(xj) satisfying the condition LCjkrvr = ηhjk, where L, hjk and Cjkr are metric function, angular metric tensor and Cartan tensor of Fn, respectively, and η is a scalar function of positional coordinates xi. Apart from obtaining expressions for different geometric objects of F* n, a subspace of F* n is studied. Apart from other results for the subspace of F* n, certain conditions for a subspace of F* n to be totally geodesic and projectively flat have been obtained. [ABSTRACT FROM AUTHOR]

Background: The dermatological condition accounts for an important public health problem of the global load of disease in low and middle-income countries and places significant pressure on primary healthcare centers. There is heterogeneity in the frequency of diverse skin conditions between different areas (1). Most of the knowledge presently available about various aspects of skin diseases is based on observations made and data collected from secondary and tertiary level hospitals (2). Information on community-based prevalence and pattern of skin diseases would be a better measure of burden due to skin diseases in general population in order to assist in the planning of possible intervention strategies (3). Aims & Objectives: To find out the prevalence, distribution, and determinants of various dermatological manifestations at the community level in Doiwala block of district Dehradun. Material & Methods: This cross-sectional study was conducted over a period of 12 months, the study subjects comprised of individuals of all age groups. A sample size of 834 (416 males & 418 females) was calculated by using current prevalence of skin diseases (43.7% in males & 44.7% in females) in Uttarakhand (1). Results: Nearly half of the studied population (45.8%) had some type of dermatological manifestations. It was slightly higher in females (47.1%) than males (44.5%). Out of 382 symptomatic respondents, 247 (64.7) had infective dermatoses followed by 124 (32.5%) non-infective, 6 (1.5%) others and 5 (1.3%) nutritional deficiency dermatoses. The gender-wise distribution of various types of dermatoses was found to be statistically significant (χ²-15.225, df-3, p-0.002). Conclusions: The prevalence of dermatological conditions in district Dehradun is fairly high with increased prevalence in the younger age group that might be an indicator to the fact that the burden of skin diseases may go to rise in the future. [ABSTRACT FROM AUTHOR]

Progressive research in the past decade converges to the impact of PHLPP in regulating the cellular metabolism through PI3K/AKT inhibition. Aberrations in PKB/AKT signaling coordinates with impaired insulin secretion and insulin resistance, identified during T2D, obesity and cardiovascular disorders which brings in the relevance of PHLPPs in the metabolic paradigm. In this review, we discuss the impact of PHLPP isoforms in insulin signaling and its associated cellular events including mitochondrial dysfunction, DNA damage, autophagy and cell death. The article highlights the plausible molecular targets that share the role during insulin-resistant states, whose understanding can be extended into treatment responses to facilitate targeted drug discovery for T2D and allied metabolic syndromes. [ABSTRACT FROM AUTHOR]

Socioeconomic scales are being used for determining the socio-economic status of study subjects which in turn is important indicator of health and nutritional status of an individual. Since BG Prasad socio-economic classification is an income-based scale and, therefore it demands constant update to take inflation and depreciation of rupee into account. Among all socio economic scales used in India BG Prasad socio-economic classification is one of most frequently used scale. BG Prasad scale was originally devised in 1961 and was later modified by himself in 1968 and 1970. These details of the calculations involved will help many researchers to calculate specific income categories for their ongoing and prospective research work in current calendar year. State-specific CPI (IW) should be used to determine more accurate income categories by researchers in community health-related studies. [ABSTRACT FROM AUTHOR]

Background: A deficient data is among the biggest obstacle facing planners and policy makers. Health data collection in the developing world is often hampered by the high costs and inefficiencies of traditional large-scale paper-based surveys. mHealth using Epi-Info is most appropriate tool to create, share, deploy health surveys and for strengthening of health systems. The program runs on free and open software, is easy to use, and can be downloaded to handheld devices to be used by workers in the field. Objectives: To find out the usefulness and limitations of data collection for mHealth by use of Epi Info™ software. Material and Methods: The devices used Epi Info 7.1.5 (Android version), which has been modeled as a database with variables of the traditional form. A cross sectional survey among adolescents regarding their health needs was carried out in a sample of 200 adolescents (purposive sampling) of rural hilly (Jaunpur block of Tehri Garhwal district) and plain (Doiwala block of Dehradun district) areas of Uttarakhand by the use of Android tablets with Epi Info™. Results: It was found that adolescent questionnaire tool developed in Epi Info™ android tablet application is a powerful tool for data collection having numerable practical advantages like: Interview Time Tracking (ITT) that gives the reality check in field studies along with cases Geographical presentation by GIS mapping. In addition to this complete filling of data in field so no left over or guessing for data entry operator, paperless, bio-friendly. Despite of Tablet cost, it is cost effective as tablet can be repeatedly used for other surveys. Conclusions: Epi Info™ is a developing open access software for primary data collection and analyzing data from the field, with advantageous benefits of epidemiological surveys. [ABSTRACT FROM AUTHOR]

Variance-preserving Power Spectra (VPS) from Research Moored Array for African-Asian-Australian Monsoon Analysis and Prediction (RAMA) observational platform depicting ocean parameters for the periods 2002-2006 at mooring location 90°E, 1.5 °S was investigated to understand the variability of ocean parameters. The VPS of 20° isothermal depth (d20) reveals fact that it has intra-seasonal, seasonal and semiannual oscillational trends. Zonal current's VPS show the intra-seasonal, biweekly and weekly characteristics. Meridional current's VPS gives biweekly and weekly oscillation and while the sea surface Zonal wind's VPS reveals fact that it has intra-seasonal, biweekly and weekly oscillation. These scientific findings are consistent with the results obtained from similar analysis of Triangle Trans Ocean Buoys Network (TRITON) data by other investigations. Therefore the use of RAMA in assimilation process in similar way to TRITON will increase the efficiency of Climate Forecast System (CFS) based on Global Ocean Data Assimilation (GODAS). [ABSTRACT FROM AUTHOR]

To enhance better understanding and improve the predictive capability for the ocean’s role in future climate change scenarios, Estimating the Circulation and Climate of the Ocean, Phase II (ECCO2) and National Centers for Environmental Prediction (NCEP) assimilation/reanalysis datasets were compared with the TRITON and RAMA mooring buoys observational data at the point 1.5ºS, 90ºE in the Indian Ocean. Root mean square deviation (rmsd) and bias values of the GODAS/NCEP reanalysis, and TRITON and RAMA observations were used for comparison of the meridional and zonal currents and salinity at the surface, and temperature at different depth of the mixed layer. On the basis of comparison we can say that the GODAS/NCEP reanalysis is not much satisfactory to use as input for the best prediction because its performance is not better in comparison to the observational data. It may also be concluded that the performance of the ECCO2 is better than the GODAS/NCEP on the basis of the comparison of the plots but still not very clear due to unavailability of equal ECCO2 temporal data set. We suggest that there might be certainly some forcing and assimilation techniques are missing to make better performance of the assimilation/ reanalysis. [ABSTRACT FROM AUTHOR]

One hundred fifty genotypes of wheat were screened for Karnal bunt resistance under artificial inoculation condition in field during 2009-10 rabi seasons. Significant variability in the incidence of disease was observed among genotypes where the per cent disease incidence ranged from 0.2-63.1 at grains harvest stage. Out of one hundred fifty genotypes screened, 12 were rated as highly resistant, 6 as resistant, 6 as moderately resistant and rest as moderately susceptible to highly susceptible genotypes against Karnal bunt of wheat. [ABSTRACT FROM AUTHOR]

• Nano-fibrous dressing with anti-oxidants for anti-scar wound healing. • Curcumin and Ce3+ protected injured tissues from ROS. • Full-thickness wound healing with no scar within 20 days. • Bioabsorption eliminates dressing removal and discomfort to patients. Open deep cuts and wounds often take a long time to heal and may cause infection and scar formation. A simple biomimetic electrospun nano-fibrous antimicrobial dressing material loaded with dual anti-oxidants has been developed to address this problem. A composite nano-fibrous material (PVP-Ce-Cur NF) comprising polyvinyl pyrrolidone (PVP), cerium nitrate hexahydrate (Ce(NO 3) 3 ·6H 2 O) and curcumin was developed using the electrospinning technique. The surface characteristics of nanofibers were examined using standard analytical techniques. The antimicrobial activity was tested against Escherichia coli (gram -ve) and Staphylococcus aureus (gram +ve) bacterial species. The MTT assay using 3T6-Swiss albino fibroblast indicated that the nano-fibrous dressing material was hemocompatible and devoid of cytotoxicity. Direct application of the PVP-Ce-Cur NF dressing material on full-thickness circular excision wound in model wistar rats displayed complete healing and re-epithelialisation without any scar within 20 days. A well-regulated level of hydroxyproline, superoxide dismutase (SOD) and catalase in granulation tissues indicated PVP-Ce-Cur NF as a potential wound dressing material with the anti-scar property. [ABSTRACT FROM AUTHOR]

Biofilms are the cause of major bacteriological infections in patients. The complex architecture of Escherichia coli (E. coli) biofilm attached to the surface of catheters has been studied and found to depend on the biomaterial's surface properties. The SEM micrographs and water contact angle analysis have revealed that the nature of the surface affects the growth and extent of E. coli biofilm formation. In vitro studies have revealed that the Gram-negative E. coli adherence to implanted biomaterials takes place in accordance with hydrophobicity, i.e., latex > silicone > polyurethane > stainless steel. Permanent removal of E. coli biofilm requires 50 to 200 times more gentamicin sulfate (G-S) than the minimum inhibitory concentration (MIC) to remove 90% of E. coli biofilm (MBIC90). Here, in vitro eradication of biofilm-associated infection on biomaterials has been done by Eudragit RL100 encapsulated gentamicin sulfate (E-G-S) nanoparticle of range 140 nm. It is 10–20 times more effective against E. coli biofilm-associated infections eradication than normal unentrapped G-S. Thus, Eudragit RL100 mediated drug delivery system provides a promising way to reduce the cost of treatment with a higher drug therapeutic index. [ABSTRACT FROM AUTHOR]

Endoplasmic reticulum (ER) dysfunction contributes greatly to the pathophysiology of hyperglycemic nephrotoxicity. This study unravels the critical role of Tribbles 3 (TRB3)-Forkhead box O1 (FoxO1) signaling pathway during hyperglycemic renal toxicity. It also uncovers the novel role of Naringenin, a flavanone, in regulating ER stress in proximal tubular cells, NRK 52E, and kidneys of streptozotocin/nicotinamide induced experimental diabetic Wistar rats. Results demonstrate that expression of ER stress marker proteins including phosphorylated protein kinase ER like kinase (p-PERK), phosphorylated eukaryotic Initiation Factor 2α (p-eIF2α), X Box Binding Protein 1 spliced (XBP1s), Activating Transcription Factor 4 (ATF4) and C/EBP Homologous Protein (CHOP) were upregulated in diabetic kidneys indicating the activation of ER stress response due to nephrotoxicity. Treatment with Naringenin reduced the expression of TRB3, an ER stress-inducible pseudokinase, both in vitro and in vivo. Gene silencing of TRB3 enhanced Akt and FoxO1 phosphorylation and alleviated FoxO1 mediated apoptosis during hyperglycemic nephrotoxicity. Notably, TRB3 gene silencing effects were comparable to the response with Naringenin treatment. Prevention of nuclear colocalization of ATF4 and CHOP in Naringenin treated cells was evident. Naringenin also reduced insulin resistance, apoptosis and glycogen accumulation along with enhancement of glucose tolerance in diabetic rats. Prevention of ultrastructural aberrations in the ER of hyperglycemic renal cells by Naringenin confirmed its anti-ER stress effects. These findings affirm that activation of TRB3-FoxO1 signaling is critical in the pathogenesis of hyperglycemia-induced renal toxicity and protective effect of Naringenin via modulation of ER stress may be exploited as a novel approach for its management. [Display omitted] • UPR sensor proteins were upregulated indicating ER stress during hyperglycemia. • Aberrant ER stress response induces TRB3 expression in vitro and in vivo. • Gene silencing of TRB3 prevented renal cell death during hyperglycemic nephrotoxicity. • Anti-ER stress effects of Naringenin were comparable to effects of TRB3 gene silencing. • Naringenin attenuated ER stress and TRB3-FoXO1 dependent responses. [ABSTRACT FROM AUTHOR]

An open wound is highly susceptible to microbial infection leading to elevated level of inflammatory response. For prompt healing, a wound requires a biomimetic dressing material with ideal hydrophilicity and tensile strength, possessing antimicrobial and antioxidant property. Although PCL-based nanofibers have sufficient tensile strength and biocompatibility, it lacks in terms of optimum hydrophilicity and biodegradation. Therefore, we fabricated a PCL-gelatin based electrospun nanofibers, enriched with quercetin and ciprofloxacin hydrochloride (CH). The average diameter of developed nanofibers was 725.943 ± 201.965 nm, and devoid of chemical interaction between two drugs and polymers. CH and quercetin exhibited biphasic in-vitro release in phosphate buffer (pH 7.4). The in-vitro antibacterial and antioxidant property of scaffolds were evaluated by film-diffusion against Staphylococcus aureus and DPPH assay, respectively. The addition of gelatin along with CH and quercetin enhanced the hydrophilicity (contact angle = 48.8 ± 2.95°) and biodegradation rate of the nanofibers. In-vitro biocompatibility of scaffold was examined by hemocompatibility and fibroblast viability using MTT assay. The results confirm the direct application of scaffold in the wounded area. Further, complete closure of full-thickness wound within 16 days, and regulation of hydroxyproline, SOD and catalase level in granulation tissues following treatment with scaffold, confirmed its application for accelerated wound healing. [ABSTRACT FROM AUTHOR]