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Innovation, which constitutes an important factor for growth, strongly diversifies regions' level of development. To compare regional innovativeness of EU territories, we employ the data envelopment analysis (DEA) framework. We analyse the relative efficiency of regions using a standard DEA for the country-level, a spatial DEA for regional analysis and the Malmquist indexes to measure the innovation productivity change over time. In our research, the inputs are human capital and R&D expenditure, while the outputs include the number of patents and GDP. We combine the results of these methods with spatial statistics to pinpoint the spatio-temporal patterns of innovation efficiency across European regions. We confirm validity of the assumed complex spatial structure of innovation efficiency. Our study indicates that innovation efficiency at the country-level is dissimilar, or even partially contradictory, to NUTS 2 outcomes due to high regional dispersion within state borers and similarities among neighbouring regions from different countries. [ABSTRACT FROM AUTHOR]

Copyright of Rozwój Regionalny & Polityka Regionalna is the property of Uniwersytetu im. Adama Mickiewicza (IH UAM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The wealth-health relationship is not unambiguous and constant. Greater wealth affects individual and population health in opposite ways. Increased risk factors especially raise the probability of noncommunicable diseases (NCDs) impacting a population. Conversely, better healthcare and awareness reduce the chances of developing these diseases or increase the likelihood of treatment and cure. Therefore, this paper aims to assess and quantify the hard-to-grasp overall impact of prosperity on mortality from selected NCDs, allowing us to capture the relevant differences between European regions. In particular, we attempt to estimate the net effect of affluence and the health economic threshold of the GDP-mortality relationship, by developing a dedicated analytical tool based on joinpoint regression and forecasting methods. Our results show that in the case of most investigated diseases in more impoverished regions, a clear pattern reflects mortality rising with prosperity. After crossing the health economic threshold of around 20 thousand euros per capita, the trend changes by stabilising or reversing. The research we present shows that health policy should be more diversified locally to enable health convergence at the national and European regional levels. Moreover, health policy should evolve to prioritise mental and neurological disorders, by improving the resource allocation and increasing public awareness. [ABSTRACT FROM AUTHOR]

Diseases of affluence (of the 21st c.) by definition should have higher prevalence and/or mortality rates in richer and more developed countries than in poorer, underdeveloped states (where diseases of poverty are more common). Therefore, it has been indicated that it is civilizational progress that makes us sick. On the other hand, substantial financial resources, highly qualified medical personnel, and the cutting-edge technology of richer states, should allow for effective preventions, diagnostics, and treatment of diseases of poverty and of affluence. Therefore, a dilemma arises: is progress making us sick or curing us? To evaluate the influence of country socioeconomic and technological development on population health, a spatial analysis of the epidemiology of diseases of affluence and distribution of economic resources for European NUTS 2 has been performed. The main aim of this paper is to assess, how regional diversity in the prevalence of diseases of affluence is related to the regional development of regions.

Featured Application: Stimuli-responsive hydrogels encompassing biobased polymers are anticipated for utilization in various fields, starting from drug delivery systems through temporal actuators and water reservoirs to biodegradable microelements and nutrient delivery depots. This paper describes the results of the radiation-induced crosslinking of polysaccharides modified with hydroxypropyl and carboxymethyl functional groups, hydroxypropylcellulose (HPC) and carboxymethylcellulose (CMC), respectively, without and with poly(ethylene glycol) diacrylate (PEGDA) as a crosslinking agent, to obtain dual stimuli-responsive hydrogels. The gels were characterized in terms of water uptake and gel fraction, parameters that mainly depend on the HPC–CMC compositions, but also on the macromer crosslinker content and the absorbed dose. The swelling of hydrogels is controlled by both the temperature, due to the amphiphilic character of HPC and pH, due to the anionic functional groups of CMC. In spite of a similar degree of substitution in both cellulose derivatives, 1.4 for HPC and 1.2 for CMC, the pH response of hydrogels with an equal content of both polysaccharides is considerably higher—a reduction in swelling of up to 95% with a decrease in the pH to 2 was recorded—than the response to thermal-stimulus—wherein a reduction in swelling of less than 70% with an increasing in temperature to 55 °C was found. These biopolymers-based hydrogels of specific, stimuli-responsive swelling properties are anticipated in applications where a combination of two stimuli is essential and biodegradation may be required. [ABSTRACT FROM AUTHOR]

Copyright of Comparative Economic Research is the property of Wydawnictwo Uniwersytetu Lodzkiego and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Epidemiological studies indicate a high risk of stroke, heart failure and myocardial infarction in patients with multiple sclerosis, especially in its secondary progressive (SPMS) phase. Some ischaemic events are directly associated with abnormal platelet functions and their prothrombotic activity. Recent reports, including this study, confirm the increased activation of circulating platelets in SPMS, and also show increased platelet reactivity, among other responses, as well as strong aggregation. In this current study, we conducted a comparative analysis of the platelet proteome in SPMS patients and in healthy controls, to demonstrate the quantitative and qualitative differences likely to affect functional changes observed in SPMS. During densitometry evaluation of 2‐D fluorescence difference gel electrophoresis, we observed differences between the electrophoretic patterns of SPMS platelets and the control samples. To determine a detailed characterisation of the proteome changes in the SPMS patients' blood platelets, in the next stage, we performed mass spectrometry of selected spots and indicated the increased presence of four proteins (fibrinogen, α‐2 macroglobulin, septin‐14 and tubulin β‐1 chain). The most important of these is the increased amount of prothrombotic protein, fibrinogen, which seems to confirm the accuracy of the imaging and potentially explains the increased risk of platelet‐origin thrombotic events. This study provides new knowledge of the potential existence of the molecular mechanisms responsible for the acceleration of the platelet pro‐coagulant function in SPMS. This can help to identify new targets for therapy, which can then be used not only in the second stage of the disease. [ABSTRACT FROM AUTHOR]

This paper examines epigenetic changes in breast cancer by Raman imaging, fluorescence imaging, AFM and SNOM and discusses how they contribute to different aspects of tumourigenesis in malignant human breast epithelial cell lines MCF7 and MDA-MB-231 compared with non-malignant MCF10A cell lines. The paper focuses on information that can be extracted from Raman microscopy and Raman imaging for the biological material of nucleoli contained within the cell nucleus and lipid droplets within the cell cytoplasm. The biochemical composition of the nuclei and lipid droplets in the non-malignant and malignant human breast epithelial cell lines has been monitored. The potential of Raman microspectroscopy to monitor acetylation processes and a prognostic value of Raman biomarkers in breast cancer have been discussed. [ABSTRACT FROM AUTHOR]

We have studied live non-malignant (MCF10A), mildly malignant (MCF7) and malignant (MDA-MB-231) breast cancer cells and human breast cancer tissue. We demonstrate the first application of Raman imaging and spectroscopy in diagnosing the role of lipid droplets in cell line cultures that closely mimic an in vivo environment of various stages in human breast cancer tissue. We have analyzed the composition of the lipid droplets in non-malignant and malignant human breast epithelial cell lines and discussed the potential of lipid droplets as a prognostic marker in breast cancer. To identify any difference in the lipid droplet-associated biochemistry and to correlate it with different stages of breast cancer, the PCA method was employed. The chemical composition of lipids and proteins, both in the cell line models and in human breast tissue has been analyzed. The paper shows the alterations in lipid metabolism that have been reported in cancer, at both the cellular and tissue levels, and discusses how they contribute to the different aspects of tumourigenesis. [ABSTRACT FROM AUTHOR]

This paper investigates the spatial process of productivity growth in the European Union on the foundations of the theory of New Economic Geography. The proposed model is based on the study of NUTS 2 regions and takes into consideration a spatial weights matrix in order to better describe the structure of spatial dependence between EU regions. Furthermore, our paper attempts to investigate the applicability of some new approaches to spatial modelling including parameterization of the spatial weights matrix. Our study presents an application of the spatial panel model with fixed effects to Fingleton's theoretical framework. We suggest that the applied approach constitutes an innovation to spatial econometric studies providing additional information hence, a deeper analysis of the investigated problem. [ABSTRACT FROM AUTHOR]

Copyright of Research Papers of the Wroclaw University of Economics / Prace Naukowe Uniwersytetu Ekonomicznego we Wroclawiu is the property of Uniwersytet Ekonomiczny we Wroclawiu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objectives Radioprotective potential of quercetin, a powerful free radical scavenger, was investigated in human red blood cells (RBCs) and in isolated RBC membranes exposed to γ-irradiation-induced oxidative stress. Methods RBCs and RBC membrane suspensions were irradiated (50 Gy) in the presence of quercetin (2–50 µM). Oxidative damage of the membranes was analysed by protein carbonyl measurement (enzyme-linked immunosorbent assay). In RBCs, the concentration of glutathione (GSH) was determined. Lipid peroxidation in RBCs, and for comparison in plasma and peripheral lymphocytes, was quantified by the amount of thiobarbituric acid-reactive substances (TBARS). Radiation-induced damage of the RBC membrane integrity was evaluated by the degree of haemolysis. Results Quercetin (50 µM) brought back the level of carbonyls to normal in γ-irradiated RBC membrane proteins and inhibited radiation-induced lipid peroxidation in plasma and lymphocytes, by 75 and 96%, respectively. However, it moderately decreased reduced/oxidized glutathione (GSH/GSSG) ratio and significantly increased TBARS concentrations, by 60 and 28% in irradiated and non-irradiated RBCs, respectively. Haemolysis rate was much higher in RBCs irradiated in the presence of quercetin vs. non antioxidant. Discussion In non-cellular systems (RBC membranes or plasma) and in lymphocytes, quercetin shows antioxidative/radioprotective activity but in whole RBCs it acts as a pro-oxidant and a cytotoxic substance. The possible mechanisms of such action are discussed. [ABSTRACT FROM AUTHOR]

Biodegradable polymers of poly(lactic acid) (PLLA) and synthesized in-house poly(trimethylene carbonate) (PTMC) with admixture of water-soluble methyl cellulose (MC) were used for development of nerve guidance tubes for peripheral nervous system regeneration after injury. Fabrication method involved phase separation of viscous dixane solution of polymers mixture seat on a rod in a proper nonsolvent, which resulted in tubular structure of large porosity. Influence of electron beam sterilization on molecular weight, thermal properties of the polymers, and mechanical performance of the tubes was evaluated. Admixture of hydrophilic MC to synthetic polymers resulted in modification of mechanical properties of the channels. Extraction of MC showed potential of the tubes for releasing water-soluble bioactive molecules, such as for instance growth factors. Basic in vitro MTT and LDH assays showed no cytotoxic effect of manufactured tubes, therefore, animal experimentations may be considered. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 [ABSTRACT FROM AUTHOR]

The present study investigates the spatially dynamic income process among 228 regions of the extended European Union 25 on the basis of the augmented Solow model. In particular spatial autocorrelation effects are taken into account. Although a lot of empirical work has been done recently on the convergence process in EU, the importance of pure spatial interactions is still largely underestimated. In this paper a spatially dynamic approach is considered to describe the driving forces present in the economic system rather than the actual movements through time. The spatial error-correction and other related models are presented and used to explain the process of regional convergence observed at the level of the EU25 NUTS 2 regions in 2004. Resumen. Este estudio investiga el proceso espacialmente dinámico de renta entre 228 regiones de la Unión Europea de los 25 en base al modelo de Solow aumentado. En particular se toman en cuenta los efectos de autocorrelación espacial. Aunque recientemente se ha realizado mucho trabajo empírico sobre el proceso de convergencia en la UE, la importancia de las interacciones espaciales puras está aun subestimada en gran parte. En este artículo se considera un enfoque espacialmente dinámico para describir las fuerzas impulsoras presentes en el sistema económico en vez de los movimientos en el tiempo. La corrección de error espacial y otros modelos relacionados se muestran y utilizan para explicar el proceso de convergencia regional observado para las regiones NUTS2 de la UE (25) en el 2004. [ABSTRACT FROM AUTHOR]

The aim of this work was to synthesize biocompatible dextran-based hydrogels through crosslinking initiated by ionizing radiation. A series of derivatives of dextran has been synthesized by coupling of methacrylated glycidyl to the structure of this polysaccharide, yielding dextran methacrylate (Dex-MA) of the degree of methacrylate substitution (DS) up to 1.13 as characterised by FTIR and NMR spectroscopy. Chemically crosslinked hydrogels were formed by electron-beam irradiation of Dex-MA in aqueous solution in the absence of low-molecular-weight additives such as catalysts, monomers or crosslinking agents. Crosslinking of Dex-MA in aqueous solutions of 20 g/l and above was an efficient process, the gels were formed at doses as low as 0.5 kGy (experiments conducted up to 100 kGy) and were characterised by high content of insoluble fraction (70–100%). Due to high crosslinking density the equilibrium degree of swelling of fabricated gels was controlled principally by the initial concentration of Dex-MA solution subjected to irradiation, and it was in the range of 20 to over 100 g of water absorbed by gram of gel. Cytocompatibility of hydrogels was examined using XTT assay through evaluation of the cell viability being in indirect contact with hydrogels. The results indicated that hydrogels of Dex-MA of the average DS below 1 were not cytotoxic. Altogether, our data demonstrate that irradiation of methacrylated dextran in aqueous solution is an efficient method of fabrication of biocompatible hydrogels, which applications in regeneration medicine are anticipated. [ABSTRACT FROM AUTHOR]

The aim of the study was to develop an antimicrobial hydrogel wound dressing by means of radiation-initiated crosslinking of hydrophilic polymers, i.e. by well-established technology comprising gel manufacturing and its sterilization in one process. The approach included admixture of chitosan of relatively low molecular weight dissolved in lactic acid (LA) into the initial regular components of the conventional hydrogel dressing based on poly(N-vinyl pyrrolidone) (PVP) and agar. Molecular weight of chitosan was regulated by radiation-initiated degradation in the range of 39–132 kg mol −1 . Optimum total concentration of LA in the resultant hydrogel dressing was evaluated as 0.05 mol dm −3 , that is ca. 0.5%. Presence of LA in the system influenced essential radiation and technological parameters of hydrogel manufacturing. The setting temperature of the pre-hydrogel mixture, resulting from agar ability to congeal, was reduced with LA concentration, yet remained significantly above the room temperature. 0.5% of chitosan was effectively dissolved in aqueous solution of lactic acid due to its pH (lower than 5.5). Radiation parameters of PVP crosslinking in the presence of LA, as determined with generalized Charlesby–Pinner equation, were reflected in slight reduction of the maximum gel fraction and increase in gelation dose and in the factor comparing yields of scission to crosslinking. Nevertheless, essentially physical characteristics of the hydrogel was not affected, except for somewhat increased water uptake capacity, what in turn improves functionality of the dressing as extensive exudate for the wound can be efficiently absorbed. Preliminary microbiological studies showed antimicrobial character of the chitosan-containing hydrogel towards Gram-positive bacterial strain. [ABSTRACT FROM AUTHOR]

The aim of the study was to investigate whether the polyphenolic-polysaccharide conjugates (PPCs), isolated from flowers of Sanguisorba officinalis L. and Erigeron canadensis L., and from leaves of Fragaria vesca L. and Rubus plicatus Whe. Et N. E., can protect human peripheral blood mononuclear cells (PBMCs) against gamma-irradiation damage while maintaining the radiosensitivity of the myeloid leukemia K562 cell line. PPCs isolated from the four plant sources are water-soluble macromolecules (14–50 kDa) that were previously chemically and structurally characterized. Cells were incubated with PPCs (25 μg/ml, 1 h) prior exposure to 15 Gy gamma-irradiation, non-irradiated appropriate samples served as controls. It was found that the PPCs were able to increase the post-radiation viability of PBMCs by inhibiting apoptosis, while they did not protect the leukemic cells against radiation-induced apoptotic death. The PPCs offered an efficient protection of PBMCs through scavenging of intracellular ROS and decreasing DNA damage, while they provided no reduction of the oxidative stress and DNA damage in K562 cells. Our findings strongly suggest that the PPCs, especially these isolated from S. officinalis and E. canadensis , can selectively protect normal lymphocytes against radiation injury, therefore they meet the criteria of radioprotectors for potential use in radiotherapy. • PPCs decrease IR-induced oxidative stress in normal lymphocytes by ROS scavenging • PPCs do not scavenge ROS generated in irradiated K562 cells • PPCs can work as radiation modifiers - efficiently protect normal lymphocytes, but not K562 cells, against radiation injury [ABSTRACT FROM AUTHOR]

Radioprotective effects of the water-soluble polyphenolic glycoconjugates, isolated from flowers of Sanguisorba officinalis L.( SO ) and Erigeron canadensis L.( EC ), and from leaves of Fragaria vesca L. ( FV ) and Rubus plicatus Whe. Et N. E. ( RP ), against γ-radiation-induced toxicity in human peripheral blood lymphocytes were investigated. Cell treatment with glycoconjugates (1, 5 and 25 μg/mL) prior exposure to 10/15 Gy radiation resulted in concentration-dependent reduction of DNA damage including oxidative DNA lesions (comet assay), substantial inhibition of lipid peroxidation (TBARS) and restoration of superoxide dismutase and S-glutathione transferase activities. Glycoconjugates isolated from SO and EC ensured better protection versus these from RP and FV , with the SO product potential comparable to that of the reference quercetin. Strong antioxidant/radioprotective activity of the SO and EC glycoconjugates could be attributed to high abundance of syringol-type and ferulic acid units in their matrices, respectively. Moreover, polyphenolic glycoconjugates (25 μg/mL), including RP and FV products, significantly decreased DNA damage when applied post-radiation suggesting their modulating effects on DNA repair pathways. Preliminary data on the glycoconjugate phenolic structural units, based on GLC/MS of the products of pyrolysis and in situ methylation, in relation to application of plant products as potential radioprotectors is promising and deserves further investigation. [ABSTRACT FROM AUTHOR]

Polyphenolic–polysaccharide macromolecular, water-soluble glycoconjugates, isolated from the selected medicinal plants of Rosaceae / Asteraceae family: from leaves of Fragaria vesca L., Rubus plicatus Whe. et N. E., and from flowering parts of Sanguisorba officinalis L., and Erigeron canadensis L., were investigated for their ability to protect proteins and lipids of human plasma against γ-radiation-induced oxidative damage. Treatment of plasma with plant conjugates (6, 30, 150 μg/ml) prior exposure to 100 Gy radiation resulted in a significant inhibition of lipid peroxidation, evaluated by TBARS levels; conjugates isolated from E. canadensis and R. plicatus and a reference flavonoid quercetin showed similar high potential (approx. 70% inhibition, at 6 μg/ml). The conjugates prevented radiation-induced oxidation of protein thiols and significantly improved plasma total antioxidant capacity, estimated with Ellman’s reagent and ABTS .+ assay, respectively. The results demonstrate by the first time a significant radioprotective capability of the polyphenolic-polysaccharide conjugates isolated from E. canadensis , R. plicatus , S. officinalis and to the less extent from F. vesca . The abilities of these substances to inhibit radiation-induced lipid peroxidation and thiol oxidation in plasma seems to be mediated, but not limited to ROS scavenging activity. [ABSTRACT FROM AUTHOR]

It is well documented that most if not all aspects of mast cell development, including growth, proliferation, and the differentiation/maturation are regulated by cytokines. Nowadays there is growing evidence that cytokines also influence the biology and function of mature tissue mast cells. Some cytokines activate mast cells directly to mediator release or modulate their reactivity to other stimulating agents. Various cytokines affect mast cells migration and expression of cell receptors, at the same time regulating the survival of tissue mast cells. Taking into account that mast cells themselves are the source of many cytokines (both preformed and newly generated) it can be assumed that these cytokines regulate the function of mast cells in tissues in autocrine manner.