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Mycotoxins pose a major problem to poultry production as a result of feed contamination which has deleterious consequences such as production losses and human health risks. A total of 158 chicken feed samples were randomly collected from 46 consenting poultry farms in Oyo State throughout the wet season (April-October; 91 samples) and the dry season (November-March; 67 samples), including compounded feed (n = 129) and feed ingredients (n = 29). Samples were promptly transported to the laboratory in sterile plastic vials for lateral flow assay for mycotoxins using six different commercial mycotoxin test kits each for aflatoxin B1, zearalenone, deoxynivalenol, ochratoxin A, fumonisin, and T-2 toxin/HT-2 toxin. Summary values of mycotoxin levels (µg/kg) in the feedstuffs were represented as frequency or median (and range). Fisher exact or Mann-Whitney U tests were carried out where appropriate at α = 0.05. Every sample contained at least four mycotoxins. Aflatoxins and fumonisin co-occurred in 80% of the samples. Aflatoxin and fumonisin concentrations were above the permissible limits in 32.9% and 18.4% respectively in feedstuff sampled in the dry season while the values were 17.1% and 6.3% respectively during the wet season. Among feed ingredients, peanut cake and maize had the highest median concentration of aflatoxin and fumonisin, respectively. Median aflatoxin concentration in the feedstuff was significantly higher than the permissible limit irrespective of season. There is a need to frequently monitor mycotoxin levels of feed and feed ingredients and improve storage system for feed ingredients in order to reduce the risk associated with high mycotoxin intake in poultry., (© 2024. The Author(s) under exclusive licence to Society for Mycotoxin (Research Gesellschaft für Mykotoxinforschung e.V.) and Springer-Verlag GmbH Germany, part of Springer Nature.)

To evaluate the risk to public health from Flaviviruses in the southwest region of the Kingdom of Saudi Arabia, we screened as sentinels, 50 commensal hamadryas baboons located at a peri-domestic site on the outskirts of Ta'if City in February 2013. Of the baboons, 12% [95% CI 5, 24], 0% [95% CI 0, 7] and 10% [95% CI 3, 22] were seropositive in a pan-Flavivirus ELISA (anti-pan-WNV 1-2, Usutu, Zika), Dengue virus 1-4 ELISA (anti-DENV 1-4) and WNV-1 PRNT, respectively, indicating Flavirus exposures of the subjects with possible risk to public health in the area., (© 2021. EcoHealth Alliance.)

Purpose: Haemoparasitic diseases are among the important factors that threaten cattle health and productivity especially in the sub-Saharan region. In Nigeria, their detection using sensitive molecular techniques is scanty. This study was designed to investigate and to reevaluate the repertoire of haemoparasites of cattle in Ibadan, Nigeria with a comparative evaluation of light microscopy (LM) and polymerase chain reaction (PCR) methods., Methods: Blood samples from 100 cattle slaughtered at Ibadan abattoirs were examined using LM and PCR techniques for haemoparasite detection. The PCR reactions using three primer sets targeting the 16S rRNA genes for Hemoplasma spp. and Anaplasma/Ehrlichia spp. and 18S rRNA genes of Babesia/Theleiria spp. were done. A few randomly selected amplicons from each set were sequenced and analysed., Results: A total infection rate of 34% by LM including Hemoplasma spp. (17%), Anaplasma spp. (16%), microfilaria (5%) and Trypanosoma spp. (12%) was recorded. While, 86% positivity was recorded with PCR amplification as follows: Hemoplasma spp. (64%), Babesia/Theleiria spp. (46%) and Anaplasma/Ehrlichia spp. (5%). Comparison of LM and PCR findings showed that no LM Anaplasma spp.-positive samples and 7 out of the 17 LM hemoplasma-positive cattle were confirmed by PCR. In addition, LM led to misdiagnosis of 46 Babesia/Theleiria spp.-positive samples. Amplicon sequencing and phylogenetic analysis of Babesia/Theileria spp.-positive samples revealed Theileria velifera and Theileria annulata. In the Anaplasma/Ehrlichia spp.-positive samples, only Anaplasma marginale was characterized. Mycoplasma wenyonii, "Candidatus Mycoplasma haemobos" and Pseudomonas fluorescens like were characterized among the hemoplasma-infected cattle., Conclusions: The first report of "Candidatus Mycoplasma haemobos" and Pseudomonas fluorescens like in Nigerian cattle is herewith documented. The alarming LM misdiagnosis of haemoparasites during this study confirms its limitations as it fails to identify many parasites and emphasizes the need for inclusion of molecular techniques to improve their detection. The study also shows for the first time the high prevalence of haemotropic mycoplasma in Nigerian cattle via molecular diagnostic methods, thus indicating a strong need for the investigation of their zoonotic implications.

Haematological and serum biochemical values are useful guides and biomarkers in health and diseases for reaching a diagnosis, estimating disease prognosis and monitoring treatment progress, in mammals. Reference ranges for some parameters differ among species of mammals and between sexes within a species. There is dearth of information on standard reference value for blood parameters for Nigerian indigenous monkeys. Whole blood and serum samples obtained from 50 apparently healthy adult monkeys in both captivity and from the wild in southwest Nigeria were subjected to haematology and serum biochemistry to obtain preliminary reference values for haematological and serum biochemical analytes for Cercocebus sebaeus (Green monkey), Cercopithecus mona (Mona monkey), Erythrocebus patas (Patas monkey) and Papio anubis (Anubis baboon). Numerical data were summarized as mean and standard deviation and subjected to statistical analysis; Student t test and analysis of variance, to compare values of blood parameters obtained between species and gender. A p-value less than 0.05 was considered significant. The hematocrit of male animals were significantly higher than that of females (P=0.01) in all the 4 species studied but there was no significant difference in other blood parameters such as total white blood cell and the differential counts, platelet count, serum aspartate transferase, alanine transferase, alkaline phosphatase, total plasma protein, albumin, and globulin concentrations between the sexes. Generally, there was no significant difference between total white blood cell and the differential counts, hematocrit, red cell count, haemoglobin concentration, platelet count, serum aspartate transferase, alanine transferase, alkaline phosphatase, total plasma protein, albumin, and globulin concentrations among the monkey species.

Lassa fever is a deadly viral haemorrhagic fever caused by Lassa Virus (LASV). Rodents, especially, Mystomys natalensis , are the known reservoirs of LASV and humans are the defined hosts. Monkeys share many illnesses with humans and experimental LASV infections in monkeys are fatal but natural LASV infection of monkeys has not been reported. Serum samples obtained between August 2015 and December 2017 from 62 monkeys belonging to six species in Southern Nigeria were tested for LASV as part of an ongoing surveillance of monkeys in the region for zoonotic pathogens. Commercially available Recombinant LASV (ReLASV) Pan-Lassa enzyme-linked immunosorbent assay (ELISA) test kits (Zalgen Labs, Germantown, MD, USA) were used to detect antibodies (IgG and IgM) and antigen specific for LASV nucleoprotein in the sera. Lassa-fever-specific IgG and IgM, and antigen specific for LASV nucleoprotein were detected in 5/62, 0/62, and 1/62 samples, respectively. The presence of LASV-specific antibodies in the sera suggests natural exposure to the virus, while the presence of LASV antigen may mean that monkeys are carriers of the virus. There is a need to broaden Lassa fever surveillance to include nonhuman primates (NHPs) for their probable role in the epidemiology of the disease., HIGHLIGHTS ., • Rodents are the natural reservoirs of Lassa fever virus (LASV) and humans are the defined hosts., • Experimental LASV infections in non-human primates (NHP) are fatal but natural infection of NHP with the virus have not been reported., • We detected antigen and antibody specific for LASV in free-living Monkeys from southern Nigeria which implies that monkeys in the region are naturally exposed to LASV and are probable carriers of the virus., Competing Interests: The authors have no conflicts of interest to declare., (© 2019 Atlantis Press International B.V.)

Ebola viruses (family: Filoviridae ) are the cause of Ebola virus disease (EVD), a highly fatal illness characterised by haemorrhagic fever syndrome in both humans and non-human primates (NHPs). West Africa was the epicentre of the 2013-2015 EVD epidemic which caused the death of over 11,000 people, including eight casualties in southern Nigeria. Antibodies to filoviruses have been detected among NHPs in some countries, but there is no documented evidence of exposures to filoviruses among NHPs in Nigeria. From August 2015 to February 2017, a total of 142 serum samples were obtained from individual captive and wild animals, belonging to 11 NHP species, in southern Nigeria, and screened for species-specific antibodies to filoviruses belonging to the species; Zaire ebolavirus [Ebola virus (EBOV)], Sudan ebolavirus [Sudan virus (SUDV)], and Marburg marburgvirus [Ravn virus (RAVV)]-using a modified filovirus species-specific ELISA technique. Of the sera tested, 2.1% (3/142) were positive for antibodies to EBOV. The entire 142 sera were negative for SUDV or RAVV. These findings point to the existence of natural exposures of NHPs in southern Nigeria to EBOV. There is need to discourage, the uncontrolled hunting of NHPs in Nigeria for public health safety., Competing Interests: None., (© Atlantis Press International B.V.)

Introduction: Buruli ulcer (BU) is a highly ranked neglected tropical disease (NTD) of global health importance with increasing incidence in sub-Saharan Africa yet there is paucity of information on the epidemiology of BU in Nigeria. Incidentally, highly BU endemic Benin Republic shares proximity with Nigeria. This study was carried out to establish presence of BU and ascertain the level of BU perception among rural populace in Ogun State, south-west Nigeria., Methods: Secondary data (2009-2012) on incidence of BU was collected from a reference hospital. A cross-sectional survey using structured questionnaire administered to rural people and healthcare practitioners was conducted in three purposively chosen Local Government Areas (LGAs) in Ogun State based on unpublished reports of BU presence., Results: Data collected revealed 27 hospital confirmed BU cases between 2009-2012 across four LGAs (Obafemi Owode, Abeokuta North, Yewa North and Yewa South) while 14%(21/150) chronic ulcers (suspected to be BU) were discovered during the cross-sectional survey carried out in Odeda, Yewa South and North LGAs. Healthcare practitioners 63.6% (42/66) and 54.7% (82/150) rural people demonstrated poor level of BU perception respectively., Conclusion: This study provides evidence that BU exists in Ogun State and evaluates the poor perception that the affected rural populace has on the disease. This pilot study presents baseline information on BU in a rural setting in Ogun State South-west Nigeria hence the vital need for prompt public health involvement and further research on the epidemiology of BU.

Accurate determination of temperature is crucial in the diagnosis of febrile conditions. Although fewer techniques have proven as useful and reliable a predictor of core body temperature as the rectal thermometry, the process of obtaining the rectal temperature could be stressful in dogs. The infrared thermometry is a noncontact device used for measuring body temperature, with advantages which include speed, convenience, and reduced stress to the animals and reduced occupational risks to the animal handler. Therefore, there is the need to assess the consistency and agreement between non-contact infrared thermometry and traditional rectal thermometry in body temperature estimation. This study compared and assessed the sensitivity of non-contact infrared thermometer used on the forehead and nasal regions respectively with that of a rectal thermometer in dogs for body temperature estimation. One hundred and thirty (130) dogs presented for veterinary attention at the Veterinary Teaching Hospital (VTH), University of Ibadan, Nigeria were enrolled in this study during August to September 2014, irrespective of sex, age, breed or health status. Temperatures of dogs presented at the clinic were obtained using both multiple non-contact infrared thermometric measures obtained in the nasal and frontal head regions; and by rectal temperature. A multivariate cross-matrix analysis was used to assess the difference in measurements between the rectal thermometry and non-contact infrared thermometry. Descriptive statistics was used to compare variation and trend regularity of the nasal and fore-head infrared thermometry. A logistic regression of the difference in measurements was computed at 95% confidence interval and P<0.05. The mean difference revealed that the rectal temperature was 5.330C higher than the non-contact infrared forehead-based temperature and 7.570C higher than nasal-based temperature measurements respectively. The Bland-Altman (B-A) plot showed that the 95% limits of agreement between the frontal and nasal obtained infrared laser thermometry methods. Temperature measure obtained using non-contact infrared thermometry (forehead and nasal region of the head) was poor in consistency and agreement compared to rectal thermometry. Usefulness of non-contact forehead infrared thermometry in routine clinical practice as a close estimate of core body temperature depends on accurate calibration and therefore not recommended.

Electrocardiographic and blood pressure measurements are extremely valuable diagnostic tools in the evaluationof the cardiovascular system of living animals. In this study, 6-lead electrocardiograms were recorded from five male captiveAfrican lions (Panthera leo). Also, blood pressure measurements were recorded and compared from three different sites;fore limb, hind limb and the tail, were recorded. Immobilization was done with a combination of Ketamine Hydrochloride(10mg/kg) and Xylazine (3mg/kg). Measurements were recorded as mean ± standard deviation. ECG readings were analysedusing descriptive statistics while blood pressure readings were compared using ANOVA at a 5% level of significance. Heartrate was 66±11.6 beats per minute. The heart rhythm was sinus in all the animals. Mean Electrical Axis (MEA) was between+810 and +930 degrees (Mean +89±5). Three animals had their MEA between +810 and +890 while two had MEA between+910 and +930. Fore limb measurements for Systolic Blood Pressure (SBP), Diastolic Blood Pressure (DAP) and MeanArterial Pressure (MAP) were 177.6±6.8 mmHg, 157.2±5.9 mmHg and 168.6±5.2 mmHg respectively. Hind limbmeasurements for the SBP, DBP and MAP were 135.4±9.5 mmHg, 120.6±5.9 mmHg and 123.0±6.8 mmHg respectivelywhile the tail measurements for the SBP, DBP and MAP were 149.6±8.3 mmHg, 132.8±5.9 mmHg and 137.2±5.8 mmHgrespectively. There was weak correlation between forelimb vs hindlimb and forelimb vs tail comparisons of SBP, DBP andMAP. However, a strong positive correlation was found between hindlimb and tail comparisons of those parameters. Resultsfrom this study should serve as a guide in the cardiovascular monitoring of captive African Lions immobilized with axylazine-ketamine combination.