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Introduction: Infections with Fasciola hepatica and Ostertagia ostertagi impinge upon dairy cow health and welfare and represent a major economic factor in livestock industry. Control measures largely rely on the use of anthelminthic drugs. However, reports of anthelmintic resistance necessitate sustainable approaches. Farmer characteristics such as attitude and personality are crucial for the implementation of control strategies and on-farm practices. Methods: In the present study, the HEXACO (Honesty-Humility, Emotionality, eXtraversion, Agreeableness, Conscientiousness, Openness to experience) model of personality structure, which conceptualizes human personality, was used to evaluate the relationship of farmer aspects with on-farm bulk tank milk seropositivity for F. hepatica and O. ostertagi. Moreover, information on farm structure, housing, management, and farmers' attitude was collected in a face-to-face interview. Farm-level seropositivity for F. hepatica and O. ostertagi was predicted via elastic net regression. Results: Out of 193 farms housing 8,774 cows in the German Federal State of Bavaria, 47 farms (24.4%) were seropositive for F. hepatica, 77 farms (39.9%) for O. ostertagi, and 42 farms (21.8%) for both endoparasites. The model for F. hepatica seropositivity selected the covariates pasture access, O. ostertagi seropositivity, higher farmer conscientiousness, and organic farming as relevant predictors. Seropositivity for O. ostertagi was predicted by F. hepatica seropositivity, pasture access, organic farming, and farmers being neutral regarding their satisfaction with animal health on their farm. Higher values for the HEXACO factors extraversion and emotionality were inversely associated with O. ostertagi seropositivity. Discussion: The present work emphasizes the importance of farmer traits in regard to animal health and parasite occurrence. For the effective acceptance and implementation of sustainable control strategies for livestock helminth infections, it is crucial to consider these aspects to holistically address the challenges of managing parasitic diseases. Moreover, tailored communication strategies can be developed incorporating the understanding of individual stockman characteristics and subsequently ensuring encouragement of stakeholders. [ABSTRACT FROM AUTHOR]

Simple Summary: Lameness is an important welfare issue in dairy farming that is causing substantial economic losses. This study aims to determine the association of potential risk factors with farm-level lameness in German dairy herds, including cubicle design and cubicle bedding, feeding management, lameness assessment, claw health management, stocking density, and floor design. Risk factors were identified for all cows regardless of the number of calvings (primiparous and multiparous cows) and for first lactation cows separately. Results of the present study showed that larger cubicle width and deep bedded cubicles are associated with a lower risk of being lame. In farms feeding a total mixed ration, the risk of being lame was lower than in farms with other feeding routines (partial mixed ration or single components). For first lactation cows, the way and frequency of lameness assessment were associated with lameness. Many of the factors revealed by this study are related to cow comfort, especially the comfort when lying down. More attention is needed here to reduce lameness in German dairy cows. Six hundred fifty-nine farms in three regions of Germany (North: n = 240, East: n = 247, and South: n = 172) were included in the study, which aims at determining the association of management-related risk factors with farm-level lameness in German dairy herds. For each risk factor, a generalised linear regression model with negative binomial distribution and logit link was built. Results showed that cows housed in deep-bedded cubicles had a lower risk of being lame than cows housed in other cubicle types. A larger cubicle width was associated with a lower risk of being lame. Feeding a total mixed ration was associated with lower lameness prevalence (compared to feeding a partial mixed ration or single components). For first lactation cows, lameness assessment performed daily (compared to less than daily) and during other work tasks (compared to lameness assessment as a separate work task) were associated with lower risk for lameness. Finally, the present study provided evidence for crucial associations of management-related risk factors with lameness in German dairy cows, especially in the fields of cubicle design, feeding management, and lameness assessment. [ABSTRACT FROM AUTHOR]

Lameness in dairy cows is an expression of pain most likely originating from a claw disorder, causing impaired animal wellbeing and substantial economic losses for farmers. The aim of this study was to investigate the effects of access to pasture, time spent on pasture, and season on farm level lameness prevalence. The survey was part of a cross-sectional observational study, in which farms in three regions of Germany (North, East and South) were visited by study veterinarians. On each farm (total: 659, N: 240, E: 247, S: 172), management data were recorded, and cows were scored for locomotion, according to Sprecher. Median farm-level lameness prevalence (Score 3/5 or higher) was 29.4% (IQR: 18.7% - 42.0%), and 8.2% (IQR: 3.7% - 14.0%) for severe lameness (Score 4/5 or higher). Farm-level lameness prevalence continuously decreased with increasing time spent on pasture (up to approximately 10 hours per cow per day). On farms that did not offer their cows access to pasture lameness prevalence did not show a seasonal variation. On farms where cows had pasture access for up to three hours per day lameness prevalence peaked in autumn. In contrast, on farms offering their cows access to pasture beyond three hours per day the peak of lameness was observed in spring. Our results revealed that even short periods of pasture access of at least two hours per cow and day (on average per year) are beneficial for the locomotion of dairy cows. [ABSTRACT FROM AUTHOR]

Fasciola hepatica is an internal parasite of both human and veterinary relevance. In order to control fasciolosis, a multitude of attempts to predict the risk of infection such as risk maps or forecasting models have been developed. These attempts mainly focused on the influence of geo-climatic and meteorological features. Predicting bovine fasciolosis on farm level taking into account farm-specific settings yet remains challenging. In the present study, a new methodology for this purpose, a data-driven machine learning approach using a random forest classification algorithm was applied to a cross-sectional data set of farm characteristics, management regimes, and farmer aspects within two structurally different dairying regions in Germany in order to identify factors relevant for the occurrence of F. hepatica that could predict farm-level bulk tank milk positivity. The resulting models identified farm-specific key aspects in regard to the presence of F. hepatica. In study region North, farm-level production parameters (farm-level milk yield, farm-level milk fat, farm-level milk protein), leg hygiene, body condition (prevalence of overconditioned and underconditioned cows, respectively) and pasture access were identified as features relevant in regard to farm-level F. hepatica positivity. In study region South, pasture access together with farm-level lameness prevalence, farm-level prevalence of hock lesions, herd size, parity, and farm-level milk fat appeared to be important covariates. The stratification of the analysis by study region allows for the extrapolation of the results to similar settings of dairy husbandry. The local, region-specific modelling of F. hepatica presence in this work contributes to the understanding of on-farm aspects of F. hepatica appearance. The applied technique represents a novel approach in this context to model epidemiological data on fasciolosis which allows for the identification of farms at risk and together with additional findings in regard to the epidemiology of fasciolosis, can facilitate risk assessment and deepen our understanding of on-farm drivers of the occurrence of F. hepatica. [ABSTRACT FROM AUTHOR]

Fasciola hepatica is one of the economically most important endoparasites in cattle production. The aim of the present work was to evaluate the relevance of production level on the associations of on-farm presence of F. hepatica with farm-level milk yield, milk fat, and milk protein in Holstein cows, a specialised dairy breed, and in Simmental cows, a dual purpose breed. Furthermore, we investigated whether differential associations were present depending on breed. Data from 560 dairy farms across Germany housing 93,672 cows were analysed. The presence of F. hepatica antibodies was determined via ELISA on bulk tank milk samples. Quantile regression was applied to model the median difference in milk yield, milk fat, and milk protein depending on the interaction of breed and fluke occurrence. Whereas a reduction in milk yield (-1,206 kg, p < 0.001), milk fat (-22.9 kg, p = 0.001), and milk protein (-41.6 kg, p <0.001) was evident on F. hepatica positive German Holstein farms, only milk fat (-33.8 kg, p = 0.01) and milk protein (-22.6 kg, p = 0.03) were affected on F. hepatica positive German Simmental farms. Subsequently, production traits were modelled within each of the two breeds for low, medium, and high producing farms in the presence of F. hepatica antibodies and of confounders. On Holstein farms, the presence of F. hepatica seropositivity was associated with lower production, while on German Simmental farms such an association was less evident. This work demonstrates that production level is relevant when assessing the associations between the exposure to F. hepatica with production characteristics. Moreover, both models indicate a breed dependence. This could point towards a differential F. hepatica resilience of specialised dairy breeds in comparison with dual purpose breeds. [ABSTRACT FROM AUTHOR]

Swellings of the ribs result from severe injury and affected animals are subjected to considerable and prolonged pain and suffering. The knowledge on rib swellings in dairy cows has yet been very limited. Therefore, the present study aimed at determining the prevalence of rib swellings in tie stall housed dairy cows in Germany as well as at identifying associated factors. Mean animal-level prevalence of rib swellings for 2,134 cows was 7.54% with a mean of 7.00% on farm level (range 0.00% - 37.49%). Multivariable mixed logistic regression models including nested random effects were built and factors associated with swellings of the ribs were evaluated for 1,740 dairy cows on 96 farms in Germany. Out of the initial 22 predictors, 8 factors were selected for the final model. Managing dairy cows on a part-time basis (OR 0.49 [CI 0.25–0.98]) appeared to decrease the odds for rib swellings compared with full-time farming. Cattle breeds other than Simmental entailed lower odds for rib swellings (OR 0.29 [CI 0.14–0.59]). Lame cows (OR 2.59 [CI 1.71–3.93]) and cows with wounds and/or swellings of the hocks (OR 2.77 [CI 1.32–5.84]) had more than two times the odds for rib swellings compared with sound animals. The results of the present study can help raising awareness of rib swellings in dairy cows and contribute to the body of evidence on this condition. [ABSTRACT FROM AUTHOR]

Fasciola hepatica and Ostertagia ostertagi are internal parasites of cattle compromising physiology, productivity, and well-being. Parasites are complex in their effect on hosts, sometimes making it difficult to identify clear directions of associations between infection and production parameters. Therefore, unsupervised approaches not assuming a structure reduce the risk of introducing bias to the analysis. They may provide insights which cannot be obtained with conventional, supervised methodology. An unsupervised, exploratory cluster analysis approach using the k–mode algorithm and partitioning around medoids detected two distinct clusters in a cross-sectional data set of milk yield, milk fat content, milk protein content as well as F. hepatica or O. ostertagi bulk tank milk antibody status from 606 dairy farms in three structurally different dairying regions in Germany. Parasite–positive farms grouped together with their respective production parameters to form separate clusters. A random forests algorithm characterised clusters with regard to external variables. Across all study regions, co–infections with F. hepatica or O. ostertagi, respectively, farming type, and pasture access appeared to be the most important factors discriminating clusters (i.e. farms). Furthermore, farm level lameness prevalence, herd size, BCS, stage of lactation, and somatic cell count were relevant criteria distinguishing clusters. This study is among the first to apply a cluster analysis approach in this context and potentially the first to implement a k–medoids algorithm and partitioning around medoids in the veterinary field. The results demonstrated that biologically relevant patterns of parasite status and milk parameters exist between farms positive for F. hepatica or O. ostertagi, respectively, and negative farms. Moreover, the machine learning approach confirmed results of previous work and shed further light on the complex setting of associations a between parasitic diseases, milk yield and milk constituents, and management practices. [ABSTRACT FROM AUTHOR]

Lameness is a tremendous problem in intensively managed dairy herds all over the world. It has been associated with considerable adverse effects on animal welfare and economic viability. The majority of studies have evaluated factors associated with gait disturbance by categorising cows into lame and non-lame. This procedure yet entails a loss of information and precision. In the present study, we extend the binomial response to five categories acknowledging the ordered categorical nature of locomotion assessments, which conserves a higher level of information. A cumulative link mixed modelling approach was used to identify factors associated with increasing locomotion scores. The analysis revealed that a low body condition, elevated somatic cell count, more severe hock lesions, increasing parity, absence of pasture access, and poor udder cleanliness were relevant variables associated with higher locomotion scores. Furthermore, distinct differences in the locomotion scores assigned were identified in regard to breed, observer, and season. Using locomotion scores rather than a dichotomised response variable uncovers more refined relationships between gait disturbances and associated factors. This will help to understand the intricate nature of gait disturbances in dairy cows more deeply. [ABSTRACT FROM AUTHOR]

Angiostrongylus vasorum is a widely distributed cardiopulmonary parasite of canids in Europe. Clinical signs in dogs can be highly variable and diagnostically challenging. A correct and early diagnosis is hence indispensable to adequately manage affected patients. First-stage larvae (L1) are excreted in the faeces of definitive hosts and conventionally identified using the Baermann technique. Moreover, ELISAs for the detection of circulating antigen and specific antibodies have been presented. The current study aimed at i) quantitatively assessing larval migration in the Baermann funnel after 12 h and 24 h; ii) investigating the influence of sample storage at 4 °C over the course of three days on the number of detected L1; iii) evaluating potential associations of adult worm burdens with larval shedding in dogs and ELISA optical density (OD) values for circulating parasite antigen and specific antibodies. Faecal samples were obtained from naturally infected dogs (n = 21) and Baermann funnels were set up in duplicate over the course of four consecutive days (days 0–3) starting with the day of sample collection. Funnels were harvested on days 1–4 after 12 and 24 h, respectively, and the number of L1 per gram faeces (LPG) was determined. The LPG did not differ between larval harvest after 12 h from harvest after 24 h. Storage of faecal samples at 4 °C for two and three days entailed a considerable decrease in LPG. Adult worm burdens and larval excretion data from previous experiments demonstrated a correlation between worm burden and LPG. In contrast, no correlations between worm burden and the level of parasite antigen and specific antibody OD values, respectively, were identified. Thus, OD values of both antigen and antibody ELISA did not allow for conclusions on infection intensity reflected by the number of adult parasites. For the detection of L1 in faeces, 12 or 24 h of larval migration time was not discriminating for A. vasorum positivity. Thus, early processing of faecal samples is essential, since larval detection and hence sensitivity of the approach considerably decreased over the course of three days of storage. Therefore, the common recommendation to collect faecal samples for three consecutive days and to subsequently analyse them needs to be reconsidered. The results of this study can be readily translated into precise recommendations for daily practice to adequately assess A. vasorum infected dogs. [Display omitted] • Baermann technique: 12 vs. 24 of larval migration is not diagnostically relevant. • Early examination of faecal samples is crucial for the sensitivity of the method. • Larval excretion reflects adult worm burden in infected dogs. • Serological ELISA OD values do not reflect adult worm burden in infected dogs. [ABSTRACT FROM AUTHOR]

Six hundred fifty-nine farms in three regions of Germany (North: n = 240, East: n = 247, and South: n = 172) were included in the study, which aims at determining the association of management-related risk factors with farm-level lameness in German dairy herds. For each risk factor, a generalised linear regression model with negative binomial distribution and logit link was built. Results showed that cows housed in deep-bedded cubicles had a lower risk of being lame than cows housed in other cubicle types. A larger cubicle width was associated with a lower risk of being lame. Feeding a total mixed ration was associated with lower lameness prevalence (compared to feeding a partial mixed ration or single components). For first lactation cows, lameness assessment performed daily (compared to less than daily) and during other work tasks (compared to lameness assessment as a separate work task) were associated with lower risk for lameness. Finally, the present study provided evidence for crucial associations of management-related risk factors with lameness in German dairy cows, especially in the fields of cubicle design, feeding management, and lameness assessment.

Dipylidium caninum is a common tapeworm of dogs. Two cases of praziquantel resistance have been described in D. caninum in the United States. No further reports have been published to the authors’ knowledge. Here, the case of a dog imported to Switzerland from Spain with a history of chronic excretion of tapeworm proglottids and unresponsiveness to praziquantel treatments is reported. Clinical signs were mild (restlessness, tenesmus, anal pruritus, squashy feces) and flea infestation could be ruled out. Infection with D. caninum was confirmed through morphological and genetic parasite identification. Different subsequently applied anthelmintic compounds and protocols, including epsiprantel, did not confer the desired effects. Proglottid shedding only stopped after oral mebendazole administration of 86.2 mg kg −1 body weight for 5 consecutive days. Clinical signs resolved and the dog remained coproscopically negative during a follow-up period of 10 months after the last treatment. This case represents the first reported apparent praziquantel and epsiprantel resistance in D. caninum in Europe. Treatment was extremely challenging especially due to the limited availability of efficacious alternative compounds.

Dicrocoelium dendriticum is a trematode colonising the bile ducts of herbivores. Coproscopic findings in dogs are usually considered gastrointestinal passages of eggs after ingestion of unheated liver tissue or infected ruminant faeces. Here, a Japanese Chin presented with diarrhoea and weight loss. Eggs comparable to D. dendriticum were detected in faeces and infection was confirmed via PCR and by ruling out differential diagnoses. Egg excretion continued for a period of 10 months. Praziquantel (50 mg/kg body weight [BW]) was administered orally for four consecutive days. Egg excretion 10 days after treatment entailed further treatments with 100 mg/kg BW, again for four days. Faecal samples were negative ten days and four weeks afterwards, diarrhoea resolved, and the dog gained weight. In cases of repeated coproscopic positivity for D. dendriticum, an infection with dogs acting as definitive hosts should be considered. Treatment with praziquantel at a higher dosage may be required., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Lameness is one of the most challenging problems in the dairy industry. Control is impeded because farmers often underestimate the number of lame cows. The objectives of this study were to assess German farmers' awareness of lameness in their herds and to determine the associations between farmers' awareness and their management practices, farm characteristics as well as with farmers' education, personality traits and attitudes. As a part of a large cross-sectional study, veterinarians visited farms in three structurally different regions of Germany: north ( n = 253), east ( n = 252), and south ( n = 260). The cows ( n = 84,998) were scored for locomotion and farmers were asked to estimate the number of cows that were lame or did not walk soundly. The ratio of farmers' estimated prevalence and the veterinarians' observed prevalence (Farmer's Detection Index; FDI) was calculated. The median lameness prevalence assessed by the veterinarians was 23.1, 39.1, and 23.2%, and the median prevalence of lame cows estimated by the farmers was 9.5, 9.5, and 7.1% in the north, east, and south, respectively. On average, farmers were conscious of only 45.3% (north), 24.0% (east), and 30.0% (south) of their lame cows. Farmers managing their herds according to organic principles had a higher FDI than farmers who managed their herds conventionally. Surprisingly, no significant associations between FDI and factors concerning claw health management could be detected. Therefore, increased awareness did not seem to be necessarily linked to improved management. Moreover, the FDI was not significantly associated with farmers' education or herd size. In the south, more extraverted farmers had a lower FDI. Those farmers who totally agreed with the statement, "I am satisfied with my herd's health," had a lower FDI than farmers who disagreed or were undecided. Moreover, farmers who disagreed or were undecided with the statement, "It affects me to see a cow in pain" had a higher FDI than those farmers who agreed to the statement. The results indicate that poor awareness of lameness was linked to the farmers' attitude and personality. Therefore, new approaches concerning the consultation regarding lameness control, such as the use of Motivational Interviewing, might be useful in the future., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Jensen, Oehm, Campe, Stock, Woudstra, Feist, Müller, Hoedemaker and Merle.)

Lameness remains a major concern for animal welfare and productivity in modern dairy production. Even though a trend toward loose housing systems exists and the public expects livestock to be kept under conditions where freedom of movement and the expression of natural behavior are ensured, restrictive housing systems continue to be the predominant type of housing in some regions. Factors associated with lameness were evaluated by application of multiple logistic regression modeling on data of 1,006 dairy cows from 56 tie stall farms in Bavaria, South Germany. In this population, approximately every fourth cow was lame (24.44% of scored animals). The mean farm level prevalence of lameness was 23.28%. In total, 22 factors were analyzed regarding their association with lameness. A low Body Condition Score (BCS) (OR 1.54 [95%-CI 1.05-2.25]) as well as increasing parity (OR 1.41 [95%-CI 1.29-1.54]) entailed greater odds of lameness. Moreover, higher milk yield (OR 0.98 [95%-CI 0.96-1.00]) and organic farming (OR 0.48 [95%-0.25-0.92]) appeared to be protectively associated with lameness. Cows with hock injuries (OR 2.57 [95%-CI 1.41-4.67]) or with swellings of the ribs (OR 2.55 [95%-CI 1.53-4.23]) had higher odds of lameness. A similar association was observed for the contamination of the lower legs with distinct plaques of manure (OR 1.88 [95%-CI 1.14-3.10]). As a central aspect of tie stall housing, the length of the stalls was associated with lameness; with stalls of medium [(>158-171 cm) (OR 2.15 [95%-CI 1.29-3.58]) and short (≤158 cm) length (OR 4.07 [95%-CI 2.35-7.05]) increasing the odds compared with long stalls (>171 cm). These results can help both gaining knowledge on relevant factors associated with lameness as well as approaching the problem of dairy cow lameness in tie stall operations., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Oehm, Jensen, Tautenhahn, Mueller, Feist and Merle.)