9 results on '"Nindia, Arlette"'
Search Results
2. First field study using Strong-LAMP for diagnosis of strongyloidiasis in Cubal, Angola
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Crego-Vicente, Beatriz, Febrer-Sendra, Begoña, Nindia, Arlette, Pessela, Agostinho, Aixut, Sandra, Martínez-Campreciós, Joan, Mediavilla, Alejandro, Silgado, Aroa, Sulleiro, Elena, Treviño, Begoña, Molina, Israel, Muro, Antonio, Salvador, Fernando, and Fernández-Soto, Pedro
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- 2023
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3. First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola
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Febrer-Sendra, Begoña, Crego-Vicente, Beatriz, Nindia, Arlette, Martínez-Campreciós, Joan, Aixut, Sandra, Mediavilla, Alejandro, Silgado, Aroa, Oliveira-Souto, Inés, Salvador, Fernando, Molina, Israel, Muro, Antonio, Sulleiro, Elena, and Fernández-Soto, Pedro
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- 2023
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4. Usefulness of real-time PCR for urogenital schistosomiasis diagnosis in preschool children in a high-prevalence area in Angola.
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Mediavilla, Alejandro, Silgado, Aroa, Sánchez-Marqués, Raquel, Bocanegra, Cristina, Nindia, Arlette, Salvador, Fernando, Pintar, Zeferino, Martínez-Vallejo, Patricia, Rubio Maturana, Carles, Goterris, Lidia, Martínez-Campreciós, Joan, Aixut, Sandra, Oliveira-Souto, Inés, Aznar-Ruiz-de-Alegría, María Luisa, Espiau, María, Molina, Israel, and Sulleiro, Elena
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NEGLECTED diseases ,SCHISTOSOMA haematobium ,PRESCHOOL children ,URINARY organs ,SCHISTOSOMIASIS - Abstract
Background: Urogenital schistosomiasis caused by Schistosoma haematobium is highly endemic in the municipality of Cubal in Angola. Currently, diagnosis is based on the observation of S. haematobium eggs in urine samples by microscopy but this method has low sensitivity. Few studies have been performed using molecular techniques in high-prevalence areas for the detection of S. haematobium. The objective of this study is to evaluate the usefulness of real-time PCR as a diagnostic technique for urogenital schistosomiasis among preschool-age children and its correlation with morbidity data. Methods: A cross-sectional study was conducted in Cubal, Angola, involving 97 urine samples from preschool-age children analyzed by the dipstick test, microscopic examination of filtered urine, and real-time PCR. The diagnosis of urogenital schistosomiasis was based on microscopy and/or real-time PCR results. Clinical and ultrasonography evaluation was performed to rule out complications of schistosomiasis. Results: We detected a total of 64.95% of samples positive by real-time PCR and 37.11% by microscopy. The sensitivity of parasitological diagnosis of urogenital schistosomiasis by real-time PCR and microscopy was 95.45% and 54.55%, respectively, and the sensitivity of real-time PCR compared with microscopy was 91.67%. A positive real-time PCR result was significantly related to older age (mean = 3.22 years), detection of eggs by microscopy, and abnormal urine dipstick results (18.56% with proteinuria, 31.96% with leukocyturia, and 31.96% with microhematuria) (p-value<0.05). Ultrasound analysis showed that 23.94% of children had urinary tract abnormalities, and it was significantly related to the real-time PCR diagnosis (p-value<0.05). Conclusions: Real-time PCR is a more sensitive technique than microscopy for urinary schistosomiasis diagnosis in preschool-age children in Cubal. This increase in sensitivity would allow earlier diagnosis and treatment, thus reducing the morbidity associated with schistosomiasis in its early stages. Author summary: Schistosomiasis is a neglected tropical disease that causes approximately 200,000 deaths worldwide each year. Urogenital schistosomiasis is caused by Schistosoma haematobium, which ultimately leads to lesions in the urinary tract due to egg deposition by the parasite. The World Health Organization recommends regular mass administration of praziquantel to prevent chronicity of the disease in high-endemicity areas. Preschool children are one of the most vulnerable groups, however, only children over two years of age are included in these strategies. The diagnostic techniques commonly used in endemic areas for egg detection by microscopy and urine hematuria have major limitations due to their low sensitivity. These limitations lead to misdiagnosis and undetected infections may eventually lead to the chronic form of the disease. Real-time PCR and microscopy were used to diagnose urogenital schistosomiasis in preschool children in Cubal, a high-transmission municipality in Angola. Urinary tract lesions were assessed by ultrasound. Of the samples tested, 64.95% were positive by real-time PCR, compared with 37.11% by microscopy, indicating sensitivities of 95.45% and 54.55%, respectively. Ultrasound analysis showed urinary tract alterations in 23.94% of the children. The results suggest that transmission of urogenital schistosomiasis in preschool children in Cubal is higher than previously estimated. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Prevalence and morbidity of urogenital schistosomiasis among pre-school age children in Cubal, Angola.
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Sánchez-Marqués, Raquel, Bocanegra, Cristina, Salvador, Fernando, Nindia, Arlette, Pintar, Zeferino, Martínez-Campreciós, Joan, Aixut, Sandra, Mossalilo, Patricia, Sulleiro, Elena, Espiau, María, Mas-Coma, Santiago, Bargues, Ma. Dolores, and Molina, Israel
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PRESCHOOL children ,SCHISTOSOMIASIS ,POOR people ,NEGLECTED diseases ,SCHISTOSOMA haematobium ,BURULI ulcer - Abstract
Background: Schistosomiasis is one of the most important neglected tropical diseases, with a great impact on public health and more than 200,000 deaths annually. Schistosoma haematobium causes urinary tract (UT) morbidity. Since schistosomiasis morbidity control programs focus on children older than 5 years, pre-school age children (PSAC) morbidity is not well known. Methods: We conducted a cross-sectional study in Cubal (Angola) among 245 PSAC with the objective of evaluating the prevalence of S. haematobium infection, the intensity of infection, and associated morbidity. For this purpose, urine filtration test followed by microscopic visualization and ultrasound examinations were performed. Results: The estimated overall prevalence of urogenital schistosomiasis was 30.2% (CI 95%; 24.5–35.9), with 20.3% (CI 95%; 15.3–25.3) of the samples analysed showing a high intensity of infection. A total of 54.5% (CI 95%; 47.6–61.8) of infected children presented UT lesions, showing a significant association between schistosomiasis infection and UT morbidity (p-value < 0.001). Bladder wall thickening was the most common lesion, being present in 100% of abnormal ultrasounds. We found that anaemia and severe malnutrition were not significantly associated with the development of UT lesions. Conclusions: S. haematobium infection in PSAC causes great UT detectable morbidities. Therefore, there is an evident need of including them in mass drug administration (MDA) campaigns and consequently the development of an adapted praziquantel treatment dosage for children under 2 years of age. Author summary: Urogenital schistosomiasis caused by Schistosoma haematobium is a major public health problem in the impoverished population of tropical regions. The guidelines of the World Health Organization determine periodic mass drug administration with praziquantel as the main strategy to prevent morbidity associated with the chronic phase of the disease. This strategy has focused in school-age children, but recent recommendations suggest including other target populations, such as pre-school age children. In this study we evaluated the prevalence and morbidity associated with urogenital schistosomiasis in Cubal, an endemic area of Angola. We found a prevalence of urogenital schistosomiasis of 30.2% (CI 95%; 24.5–35.9) with a high infection intensity of 20.3% (CI 95%; 15.3–25.3) among pre-school age children. In addition, among the children infected, 54.5% (CI 95%; 47.6–61.8) presented urogenital abnormalities on ultrasound examination. There is an evident need of including them in mass drug administration campaigns and consequently the development of an adapted praziquantel treatment dosage for children under 2 years of age. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Schistosoma haematobium infection and morbidity risk factors for pre-school age children in western Angola: A knowledge, attitudes and practices survey.
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Sánchez-Marqués, Raquel, Salvador, Fernando, Bocanegra, Cristina, Nindia, Arlette, Pintar, Zeferino, Martínez, Joan, Aixut, Sandra, Pessela, Agostinho, Ramírez-Arroyo, Sheila, Farrés, Aina, Chopo, María, Izquierdo, Silvia, Mas-Coma, Santiago, Bargues, María Dolores, and Molina, Israel
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PRESCHOOL children ,SCHISTOSOMA haematobium ,PARASITIC diseases ,JUVENILE diseases ,SYMPTOMS - Abstract
Background: Urogenital schistosomiasis is one of the most prevalent parasitic diseases in sub-Saharan Africa. It is a poverty-related disease conditioned by behavioural practices. Methods: Our objective is to evaluate the awareness, mindset and habits about urogenital schistosomiasis in the community of Cubal (Angola), as well as its association with infection and urinary tract morbidity in pre-school age children. A cross-sectional study of knowledge, attitudes and practices at home was conducted between February and May 2022 with 250 participants. Results: Overall, 93.6% of those surveyed had some prior knowledge about schistosomiasis and, among all the symptoms associated with this disease, blood in the urine was the best known (54.4%). Nevertheless, 57.6% obtained a medium knowledge score. Regarding attitude, the majority of respondents had a high attitude score (79.2%) with 96.0% willing to participate in mass drug administration campaigns. Laundry in the river was the most common risk practice (61.2%) and 55.2% out of the total were classified with a low practice score. Conclusion: Low knowledge about symptoms and transmission by caregivers was the outstanding risk factor for infection in pre-school age children (OR = 16.93, 95%CI: 3.93–72.82), and lack of knowledge that avoiding entering the river prevents schistosomiasis was the main risk factor for morbidity in PSAC (OR = 8.14, 95%CI: 1.14–58.25). Author summary: Schistosomiasis is one of the most prevalent parasitic diseases and, due to its transmission by infested water, it is particularly linked to the poverty of the populations suffering from it, since most of them do not have easy access to running water. The main activities aimed at interrupting the parasite cycle require community participation and acceptance for their implementation, and for this purpose, being aware of the practical, cultural and social determinants of the disease in a particular population is essential. In this sense, KAP (knowledge, attitudes and practices) surveys may reveal misconceptions or misunderstandings that could represent obstacles to the health empowerment of the population at risk and potential barriers to behaviour changes. This study shows the results of a baseline KAP survey in a region in Angola of high endemicity for urinary schistosomiasis, showing that the population's knowledge of the conditioning factors of the disease is intermediate and that poor knowledge and risk practices of caregivers of preschool children can represent a risk factor both for the infection of the children and for the development of the disease and its associated morbidity. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Ultrasound Evolution of Pediatric Urinary Schistosomiasis after Treatment with Praziquantel in a Highly Endemic Area.
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Bocanegra, Cristina, Pintar, Zeferino, Mendioroz, Jacobo, Serres, Xavier, Gallego, Sara, Nindia, Arlette, Aznar, Maria Luisa, Soriano-Arandes, Antoni, Salvador, Fernando, Gil, Eva, Sikaleta, Nicolau, Moreno, Milagros, and Molina, Israel
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- 2018
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8. Prevalence of Strongyloides stercoralis and Other Intestinal Parasite Infections in School Children in a Rural Area of Angola: A Cross-Sectional Study.
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de Alegría, María Luisa Aznar Ruiz, Colmenares, Karen, Espasa, Mateu, Amor, Arancha, Lopez, Isabel, Nindia, Arlette, Kanjala, Joaquina, Guilherme, Domingas, Sulleiro, Elena, Barriga, Begoña, Gil, Eva, Salvador, Fernando, Bocanegra, Cristina, López, Teresa, Moreno, Milagros, and Molina, Israel
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- 2017
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9. Epidemiology of Schistosomiasis and Usefulness of Indirect Diagnostic Tests in School-Age Children in Cubal, Central Angola.
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Bocanegra, Cristina, Gallego, Sara, Mendioroz, Jacobo, Moreno, Milagros, Sulleiro, Elena, Salvador, Fernando, Sikaleta, Nicolau, Nindia, Arlette, Tchipita, Daniel, Joromba, Morais, Kavaya, Sebastiao, Sánchez Montalvá, Adrián, López, Teresa, and Molina, Israel
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SCHISTOSOMIASIS ,ROUTINE diagnostic tests ,HELMINTHIASIS ,DISEASE risk factors - Abstract
Introduction: Schistosomiasis remains a public health major problem and little is known in many areas, mainly in Sub-Saharan Africa Objectives: To assess the burden and risk factors of schistosomiasis and intestinal parasitic helminthes in the children of Cubal, Angola, and to compare different diagnostic approaches for urinary schistosomiasis under field conditions. Methods: A cross-sectional study was conducted. Urine and faeces samples of school children were microscopically studied. A random sample of children was obtained from an alphabetically arranged list of children, taking one of two children. Urine dipstick, colorimetric test and macrohaematuria were considered as indirect diagnostic methods and compared to direct urine examination. Possible risk factors for the infection were sex, age, distance to the river and previous treatment with praziquantel; the assessment was performed using Chi-square test. Results: A total of 785 (61.18%) children showed S. haematobium eggs in urine; children living within 500 meters from the river had a higher odds for infection: Odds ratio 1.97 (1.45–2.7 CI 95%); urine dipstick showed sensitivity of 96% and specificity of 61.3%, with a positive predictive value; colorimetric test showed sensitivity of 52.5%, specificity of 74.6% and a positive predictive value of 77%. Proteinuria was present in 653 (51.1%) children, being more frequent in children with S. haematobium in urine (75.2%); 32 of 191 stool samples (16%) showed the presence of other intestinal parasites and 8 (4%) for S. haematobium. Conclusions: Prevalence of urinary schistosomiasis in our study area is much higher than the national average, considering it as a high-risk community. Proximity to a source of water was a risk factor for the infection. Indirect tests, as urine dipstick and colorimetric test, were useful tools for diagnosis, due to ease of use and low cost. Proteinuria was a common finding, probably showing an early structural damage due to schistosomiasis in this group of children. [ABSTRACT FROM AUTHOR]
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- 2015
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