Search

Anthracnose disease is one of the major causes of yield losses around 45 to 85% in cowpea crops production in Cameroon. Synthetic fungicides are commonly used for diseases management, yet concerns exist regarding their cost effectiveness and environmental impact. This study aimed to control the development of cowpea anthracnose by using extracts from the seeds of Balanites aegyptiaca (L.) Del. The experimental design was a “split-plot” with four replicates. The varieties constituting the main plots randomly in replication with two variants: V1 (Tiligré) and V2 (Lori 24-130) and treatments represented sub-plots randomized within the main plot (T0: control; T-AqE: aqueous extract; T-AcE: acetone extract; T-ME: methanol extract; T-Fong: synthetic fungicide containing 80% of maneb) during two campaigns (2020 and 2021). As results, the plots treated with synthetic fungicide T-F: (9.78 and 9.1%), aqueous extracts (10.6 and 20.48%) and acetone extracts (12.2 and 12.3%) recorded lower severity than control plots (64.14 and 59.82%) for varieties V1 and V2, respectively. The yields were higher in plots treated with aqueous extract (794.05 and 926.63 kg ha-1) and lower in the control plots (518.03 and 545.055 kg ha-1) respectively for the varieties V1 and V2. Balanites aegyptiaca (L.) seed extracts could be used in the integrated control of cowpea anthracnose disease.

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is a critical antimicrobial resistance threat and a WHO-prioritized pathogen. With intrinsic resistance to multiple antibiotics and the emergence of pan-resistant isolates, CRAB infections are challenging to treat, often relying on polymyxins, tigecycline, aminoglycosides, or combinations, though co-resistance is rising globally. Phage therapy is considered as a potential treatment for multidrug-resistant A. baumannii. This study focused on isolating and characterizing phages active against CRAB strains from healthcare-associated infections in Dakar, Senegal. Methods: A lytic phage, Acinetobacter vAbaIN10, was isolated from wastewater collected at the Aristide Le Dantec Hospital in Dakar, Senegal. Isolation, host range, efficiency of plating, temperature and pH stability, lysis kinetics, one-step growth test, sequencing, and genomic analysis were performed. Results: Phage vAbaIN10 belongs to the class Caudoviricetes and the genus Friunavirus. Its genome is 40,279 bp in size. Phage vAbaIN10 is stable across a wide pH range (3–9) and temperature range (25°C–60°C). The phage's lytic activity was evaluated at different multiplicities of infection (MOI): MOI 10, 1, and 10⁻¹. All MOIs significantly reduced the growth of host bacteria. The one-step growth curve showed that vAbaIN10 had a latency period of 25 min and a burst size of approximately 4.78 × 10³ phages per infected bacterial cell. No tRNA, mtRNA, clustered regularly interspaced short palindromic repeat, virulence factors, or antibiotic resistance genes were found in the genome. Conclusions: The biological and genomic characteristics of vAbaIN10 meet the requirements for its potential use in phage therapy.

Enterovirus D68 (EV-D68) has emerged as a major public health concern because of its association with outbreaks of severe acute respiratory illness (SARI), acute flaccid myelitis (AFM), and acute flaccid [...]

The need to improve diversity, equity, inclusion, and accessibility (DEIA) efforts has reached monumental heights in the past decade as a result of the Black Lives Matter movement and other DEIA efforts. This shift in thought has impacted recruitment and hiring practices in the public, private, and nonprofit sectors. For years, universities have tried a variety of mechanisms to increase the pool of minority applicants in PhD programs as well as the faculty ranks. However, many of these efforts have not been sustainable. In this article, we discuss the results from a survey of graduate-level public affairs programs to discern specific efforts to increase the number of minority students pursuing graduate degrees and subsequently the number of minority faculty. In addition, we completed an extant review of the literature to determine additional practices that have proven fruitful in other graduate programs.

Carica papaya pseudostems are widely available as biomass waste in Cameroon. These agricultural wastes can be effectively used as natural fibers in the manufacture of biocomposites. In this study, Carica papaya fibers were extracted from papaya pseudostems by retting with water and an alkaline sodium hydroxide (NaOH) solution at different concentrations (2.5 %, 5 %, and 7.5 %). An experimental campaign is being conducted on the physical, chemical, thermal, mechanical, and morphological characteristics of Carica papaya fibers. Fourier transform infrared spectroscopy (FTIR) of Carica papaya fibers extracted by water retting and those extracted with NaOH indicates that the cellulose, hemicellulose, and lignin functional groups are present in the fibers and are dissolved considerably as the percentage of NaOH increases. Scanning electron microscopy (SEM) in the longitudinal plane gives a visual representation of the rough and irregular surfaces without the presence of impurities on the chemically extracted fibers compared to that extracted with water. In contrast to the decrease in diameter, the measured density of Carica papaya pseudostem fibers increased with NaOH concentration (0.633 –1.522 g cm−3), all of which remained light fibers. Water absorption decreased from 159.36 % to 141.28 % with increasing NaOH, and relative humidity dropped to 6.41 %. The thermal stability of Carica papaya fibers extracted at concentrations of 2.5 % NaOH (215 °C), 5 % NaOH (200 °C), and 7.5 % NaOH (175 °C) showed a clear decrease compared to those extracted by water retting (220 °C). Fibers extracted at 2.5 % NaOH achieved a tensile strength of 287.55 ± 56 MPa, a tensile modulus of 8.271a± 1.62, and 3.505 ± 1.01 % elongation. The tensile properties of these fibers showed great variability, and an influence of diameter was observed, indicating the need to study the influence of technique and NaOH mass concentration. The results show that Carica papaya fiber extracted at a concentration of 2.5 % will be the most suitable for biocomposite applications.

A fatal case of Crimean-Congo hemorrhagic fever virus (CCHFV) was detected in Dakar, Senegal, West Africa, in April 2023. To elucidate the mechanisms involved in this outcome, the kinetics of viral load, immunoglobulins, and cytokines were assessed using RT-qPCR and ELISA. A high IL-6 titer, low IL-10 titer, and delayed IgG response were associated with disease severity. These biomarkers may serve as predictors of disease severity, potentially aiding in early intervention and treatment strategies.

Chikungunya virus has caused millions of cases worldwide over the past 20 years, with recent outbreaks in Kedougou region in the southeastern Senegal, West Africa. Genomic characterization highlights that an ongoing epidemic in Kedougou in 2023 is not due to an introduction event but caused by the re-emergence of an endemic strain evolving linearly in a sylvatic context.

Summary: Background: Infection prevention and control (IPC) helps prevent disease transmission in healthcare facilities. There is a dearth of information on the implementation of IPC during the COVID-19 outbreak in Cameroon using the recommended WHO COVID-19 IPC scorecard tool. The present study assessed healthcare facilities' compliance to IPC by continuous assessments, with an evaluation of the tool using the hierarchy of control theory. Methods: This cross-sectional study was conducted in the 10 administrative regions of Cameroon by evaluating healthcare facilities prioritized by the Ministry of Public Health as high-risk facilities between March 2020 and November 2023. Comparisons were made regarding the facilities' ownership, level and status. Results: 2,188 assessments from 1,358 healthcare facilities were collected. The median IPC scores at each evaluation were between the intermediate and advanced level, with a bias linked with decreasing selection of facilities. However, only 172 (13%) healthcare facilities achieved advanced IPC score (≥75%). Higher IPC scores were found in hospitals (p

Summary: Objectives: Infection prevention and control (IPC) contributes to the reduction of healthcare associated infections. Notwithstanding the global attention with available guidelines and tools, low- and middle-income countries (LMICs) still struggle to put into place effective IPC programmes. Here, we use a socioecological approach to summarize the findings of a recent workshop on the implementation of IPC activities in Cameroon. Study design: We conducted a cross-sectional study on the assessment of the IPC in Cameroon. Methods: Experts and key stakeholders involved in IPC in Cameroon evaluated the implementation of infection prevention and control during a 4-day workshop. Detailed summaries of workshop discussions and recommendations were created. Data were clustered into themes guided by the WHO core component of IPC. Results were analyzed using the socioecological model of Bronfenbrenner, McLeroy and the theory of Grol and Wensing on successful implementation of practices in healthcare settings. Results: Cameroon does not have an effective IPC programme in place but has developed some areas of the World Health Organization (WHO) IPC core components across the guideline level, the individual level, the organizational level, and the political level. Conclusion: Cameroon is still far from the norms and standards laid out by the WHO. The evidence generated from the current analysis should contribute to improve policies and strategies towards an effective IPC programme in Cameroon and other LMICs.

The second edition of the Conference on Economic and Monetary Sovereignty of Africa was held in Dakar, Senegal, on October 25–28, 2022. Participants from around the world debated the theme “Facing the Socio-Ecological Crisis: Delinking and the Question of Global Reparations.” The event was designed as a follow-up on discussions begun during the first edition (held in Tunis in 2019; see Ben Gadha et al. 2021), as well as an opportunity to reflect on recent developments.  The Dakar Declaration is one of the main byproducts of four days of intense, fruitful, and comradely debates on the triptych of delinking, socio-ecological resilience, and reparations. It is an internationalist manifesto and a global action plan.

Despite extensive experience with influenza surveillance in humans in Senegal, there is limited knowledge about the actual situation and genetic diversity of avian influenza viruses (AIVs) circulating in the country, hindering control measures and pandemic risk assessment. Therefore, as part of the “One Health” approach to influenza surveillance, we conducted active AIV surveillance in two live bird markets (LBMs) in Dakar to better understand the dynamics and diversity of influenza viruses in Senegal, obtain genetic profiles of circulating AIVs, and assess the risk of emergence of novel strains and their transmission to humans. Cloacal swabs from poultry and environmental samples collected weekly from the two LBMs were screened by RT-qPCR for H5, H7, and H9 AIVs. Subsequently, a subset of H9-positive samples was selected for whole sequencing. From December 2023 to October 2024, 499 samples were tested, and AIV was detected in 58.3% of them. Among these, A/H9N2 was the only subtype detected in both markets, with a detection rate of 47.7% (82/172) in Thiaroye and 35.3% (42/119) in Tilene, resulting in an overall positivity rate of 42.6% (124/291). Genome sequencing of 22 A/H9N2 isolates, including 11 poultry drinking water samples, 7 carcass wash water samples, 3 fecal samples, and 1 cloacal swab, yielded 7 complete and 15 partial genomic sequences. Phylogenetic analyses of the resulting sequences showed that the A/H9N2 isolates obtained in this study formed a monophyletic cluster and were closely related to the Senegalese human strain (A/Senegal/0243/2019) identified through the national influenza sentinel surveillance program. These strains were also closely related to the A/H9N2 viruses of the G1 lineage circulating in neighboring countries, suggesting cross-border transmission. The A/H9N2 strains carried the low pathogenicity RSSR/GLF motif at the HA cleavage site and possessed several key amino acid mutations, including HA-I155T and HA-Q226L, which are associated with human host adaptation, PB2-T105V, PB2-A661T, and PB2-A588V, which are linked to the human-to-human transmission and increased polymerase activity, NS2-T14M, NS2-M100I, NS1-I106M, NS1-V222M, NS1-E223A, NS1-I226V, NS1-E227G, and NS1-P228S, which are known to alter virulence (increased or reduced) in humans or mice, and M2-S31N, which promotes drug resistance. Seven potential N-glycosylation sites were predicted in the HA protein and six in the NA protein. The selection pressure analysis revealed that the A/H9N2 isolates were primarily under neutral evolution or purifying selection pressure. Overall, our findings highlight the potential for cross-species transmission of Senegalese A/H9N2 viruses, emphasizing the need for sustained monitoring of these viruses in both animal and human populations.

Neurological manifestations associated with human parvovirus B19 (B19V) infections are rare and varied. Acute encephalitis and encephalopathy are the most common, accounting for 38.8% of all neurological manifestations associated with human B19V. Herein, we report on the clinical features of 13 laboratory-confirmed human cases of B19V-associated encephalitis in Senegal in the framework of a hospital-based surveillance of acute viral encephalitis conducted from 2021 to 2023. Overall, B19V was detected from 13 cerebrospinal fluid samples using specific real time PCR. The mean age was 16.7 years among B19V-positive patients, with a higher prevalence in 0–5-year-old children and the sex ratio (male/female) was 2.25. The B19V-positive patients mainly exhibited hypoleukocytosis, normal glycorrhachia, and normal proteinorrachia in the cerebrospinal fluid. While the main neurological symptoms included meningeal and infectious syndromes. Furthermore, three complete B19V genome sequences were successfully characterized using next-generation sequencing. The newly characterized sequences belonged to the genotype 1a and represent, to date, the first complete B19V genome sequences from Senegal. These sequences could be useful not only in future phylodynamic studies of B19V but also in the development of prevention or treatment countermeasures. Our study is noteworthy for the identification of acute B19V-associated encephalitis in Senegal More investigations on the risk factors associated with B19V transmission in Africa are warranted.

Summary: Background: Contact tracing was described as a key strategy to contribute to controlling the spread of severe acute respiratory syndrome of Coronavirus 2 (SARS-CoV-2) but implementing it can be a challenge. Digitalisation of contact tracing is among the proposed solutions being explored in sub-Saharan African settings. We assessed the effectiveness of a digital tool to expand SARS-CoV-2 testing in exposed individuals in Cameroon. Methods: We conducted a cluster-randomised (1:1) trial in eight health districts, including 22 facilities and SARS-CoV-2 testing units, randomly assigned to a digital (intervention) or standard (control) contact tracing approach. The intervention consisted of a contact tracing module added to the digital platform “Mamal PRO” used for monitoring and coordination of Coronavirus Disease 2019 pandemic response in Cameroon. The primary outcome was the proportion of contacts declared by SAR-CoV-2 index patients who were successfully traced and tested for SARS-CoV-2 evaluated with a Poisson regression model with cluster adjustment. This study is registered with ClinicalTrials.gov (NCT05684887). Findings: Between October 18, 2022, and March 31, 2023, we enrolled 164 index patients in the intervention arm and 149 in the control arm, who identified 854 and 849 contacts, respectively. In the intervention arm, 93.8% (801/854) of identified contacts were successfully reached by the tracing unit versus 54.5% (463/849) in the control arm. The intervention significantly increased the likelihood of successfully tracing contacts (adjusted relative risks (RR) 1.72 [95% CI: 1.00–2.95], p = 0.049). The median (interquartile range, IQR) time to successfully tracing contacts was 0 days [IQR: 0, 1] in the intervention and 1 day [IQR: 0, 2] in the control arm. In the intervention arm, 21.3% (182/854) of identified contacts received SARS-CoV-2 testing compared to 14.5% (123/849) in the control arm (adjusted RR 1.47 [95% CI: 0.44–4.90], p = 0.530). Interpretation: Digitalising the contact tracing process improved exposure notification and facilitated the tracing of a greater number of contacts of individuals infected with SARS-CoV-2 in resource-limited settings. Funding: The study was funded by FIND, United Kingdom (FCDO 40105983), Switzerland (81066910), Netherlands (SDD 4000004160), Canada (DFATD 7429348), The Kingdom of Saudi Arabia (FIND—ACT-A DX PARTNERSHIP 20.08.2020), The Rockefeller Foundation (2020 HTH 059), Germany (BMZ Covid-19 Diagnostic and Surveillance Response 27.07.2021), Australia (DFAT 76442), Kuwait (M239/2020), The Government of Portugal and Partners (ANF, BCP, CGF, APIFARMA) and The BlackRock Foundation (Grant Agreement as of April 20, 2022).

In temperate regions, seasonal influenza commonly follows a regular circulation pattern and has an annual epidemic peak during the colder winter months (1-3). In contrast, tropical areas have great diversity [...]

Objectives: Several factors can cause acute flaccid paralysis cases including non-polio enteroviruses. In Senegal, few studies on non-polio enteroviruses (NPEV) have been performed. Methods: Our study assess the molecular epidemiology of non-polio enteroviruses in Senegal from 2013 to 2021 through the previously existing programs for surveillance of polioviruses. Results: A total of 3815 stool samples and 281 sewage samples were collected. After virus isolation by cell culture, non-polio enteroviruses-positive isolates were confirmed by reverse transcriptase-quantitative polymerase chain reaction. Following this detection, the positive samples were subjected to molecular characterization. Our data showed that 15.22% and 52.66% were positive in cell culture for non-polio enteroviruses in acute flaccid paralysis surveillance and environmental surveillance, respectively. These non-polio enteroviruses-positive isolates were detected all year round but tend to unequal peaks of circulation, and the age group 0-5 years was more vulnerable to infection (84.4%). Genetic characterization revealed the circulation of enteroviruses species infecting humans (Enterovirus A – Enterovirus D): Enterovirus A (29.2%) and Enterovirus B (63.1%) isolates from both the acute flaccid paralysis surveillance and environmental surveillance while Enterovirus C (5.3%) and Enterovirus D (2.4%) were only isolated from the acute flaccid paralysis surveillance. However, the highly prevalent Enterovirus B species from the acute flaccid paralysis surveillance included echovirus 7 and echovirus 13, whereas coxsackievirus A6 was the predominant species from the environmental surveillance. Conclusion: This first 8-year period study of NPEV in Senegal showed that NPEV represent important viral etiologies associated with acute flaccid paralysis cases and circulating in environmental surveillance in Senegal and highlighted the need to promote effective long-term strategies for monitoring of non-polio enteroviruses infections.

Abstract While the SARS-CoV-2 dynamic has been described globally, there is a lack of data from Sub-Saharan Africa. We herein report the dynamics of SARS-CoV-2 lineages from March 2020 to March 2022 in Cameroon. Of the 760 whole-genome sequences successfully generated by the national genomic surveillance network, 74% were viral sub-lineages of origin and non-variants of concern, 15% Delta, 6% Omicron, 3% Alpha and 2% Beta variants. The pandemic was driven by SARS-CoV-2 lineages of origin in wave 1 (16 weeks, 2.3% CFR), the Alpha and Beta variants in wave 2 (21 weeks, 1.6% CFR), Delta variants in wave 3 (11 weeks, 2.0% CFR), and omicron variants in wave 4 (8 weeks, 0.73% CFR), with a declining trend over time (p = 0.01208). Even though SARS-CoV-2 heterogeneity did not seemingly contribute to the breadth of transmission, the viral lineages of origin and especially the Delta variants appeared as drivers of COVID-19 severity in Cameroon.

The 800-m (m) run is part of Physical Education classes in Cameroon, after which arrhythmias may occur during recovery. Hence, this study aimed at determining relationship between 800-m run loads on cardiac autonomic recovery among school adolescents.Forty-two male adolescents (aged [17 ​± ​1] years) performed 800-m. Post-exercise heart rate variability (HRV) was recorded during 5-min (min) (HRV5-min) and 15-min (HRV15-min) in time: Standard deviation of normal to normal (SDNN); Root mean square of successive differences (RMSSD) and frequency domain (LH: Low frequency, HF: High frequency, TP: Total power). Rating of Perceived Exertion (RPE) and blood lactate concentration (BLa) were measured after exercise. In HRV5-min, RPE was associated with SDNN (r ​= ​−0.44, p ​

Considérée comme un mode d’expression privilégié de la souveraineté, la norme reste encore une énigme pour certains pays en émergence. A ce titre, les pays occidentaux ont développé leurs industries de BTP et des matériaux de construction en se basant sur les normes ; celles-ci pour la maitrise des phénomènes d’étalement urbain et de bidonvilles, répondant ainsi aux besoins de construction exprimés par la population. Certains pays en voie d’émergence comme la République du Congo ne disposent malheureusement pas de normes nationales dans le domaine du Bâtiment et Travaux Publics. Sur ce, leurs parcs immobiliers sont souvent envahis en grande partie par des produits et services non adaptés à l’usage auquel ils sont destinés. N’ayant pas et/ou peu de choix de ce qui leur est offert, les consommateurs se trouvent exposés au danger. De ce fait, cette étude souligne la nécessité d’élaborer les normes de conception, de réalisation et d’exploitation des ouvrages du Bâtiment et des infrastructures afin d’empêcher l’importation de mauvaises pratiques douteuses inadaptées aux conditions du Congo-Brazzaville. Pour ce faire, l’étude dresse premièrement un état des lieux des normes de construction au niveau international. Deuxièmement, nous allons introduire le concept de normes de construction congolaise. Enfin, il sera question de discuter de l’élaboration et de l’harmonisation des normes techniques (ou métiers) dans l’industrie du Bâtiment et des Infrastructures au Congo Brazzaville.

Echovirus 11 has emerged as a major public health concern, causing sepsis in neonates in many European countries in recent years. In Africa, especially West Africa, where resources and diagnostic capacities are limited, only sporadic cases have been reported. To better understand the recent molecular epidemiology of E11 in West Africa, we characterized twenty-three echovirus 11 strains isolated through the acute flaccid paralysis and environmental surveillance systems for polio from 2013 to 2023, using high-throughput sequencing. Our data are noteworthy due to identifying for the first time a recombinant strain from an acute flaccid paralysis case and represent the first focus to date on molecular characterization of echovirus 11 in West Africa. Moreover, our data show that echovirus 11 diverged from 1970 (95% HPD range, 1961–1979) and evolved into four distinct clades, with the virus spread from West Africa to Europe, exhibiting two introductions in France around 2017, from Senegal and Guinea. Furthermore, the in silico analysis reveals four non-conservative amino acid substitutions in the VP1 sequences of the European strains associated with neonatal sepsis in newborns and a conserved amino acid motif in the VP1 protein toward enterovirus genotypes. Our data provide new insights into the epidemiology of echovirus 11 and point to the crucial need to implement specific surveillance programs targeting non-polio enteroviruses for the rapid identification of emerging or re-emerging enterovirus species, particularly in Africa.

Mortality in children accounts for 15% of all AIDS-related deaths globally, with a higher burden among Cameroonian children (25%), likely driven by poor virological response. We sought to evaluate viral suppression (VS) and its determinants in a nationally representative paediatric and young adult population receiving antiretroviral therapy (ART). A cross-sectional and multicentric study was conducted among Cameroonian children (p < 0.001. VS was 85.1% on a DTG-based regimen versus 80.0% on efavirenz/nevirapine and 65.6% on lopinavir/ritonavir or atazanavir/ritonavir. VS was higher in females versus males (85.8% versus 78.2%, p < 0.001). The VS rate remained stable around 85% at 12 and 24 months but dropped to about 80% at 36 months after ART initiation, p < 0.009. Independent predictors of non-VS were younger age, longer ART duration (>36 months), backbone drug (non-TDF/3TC) and anchor drug (non-DTG based). In this Cameroonian paediatric population with varying levels of transition to DTG, overall VS remains below the 95% targets. Predictors of non-VS are younger age, non-TDF/3TC- and non-DTG-based regimens. Thus, efforts toward eliminating paediatric AIDS should prioritise the transition to a DTG-based regimen in this new ART era.

Abstract In 2022, many regions around the world experienced a severe respiratory syncytial virus (RSV) epidemic with an earlier-than-usual start and increased numbers of paediatric patients in emergency departments. Here we carried out this study to describe the epidemiology and genetic characteristics of RSV infection in patients hospitalized with severe acute respiratory infections in 2022. Samples were tested for RSV by multiplex real time reverse transcription polymerase chain reaction. Subsequently, a subset of RSV positive samples was selected for NGS sequencing. RSV was detected in 16.04%, among which RSV-A was confirmed in 7.5% and RSV-B in 76.7%. RSV infection were more identified in infants aged ≤ 11 months (83.3%) and a shift in the circulation pattern was observed, with highest incidences between September–November. Phylogenetic analyses revealed that all RSV-A strains belonged to GA2.3.5 genotype and all RSV-B strains to GB5.0.5a genotype. Three putative N-glycosylation sites at amino acid positions 103, 135, 237 were predicted among RSV-A strains, while four N-linked glycosylation sites at positions 81, 86, 231 and 294 were identified in RSV-B strains. Globally, our findings reveal an exclusive co-circulation of two genetic lineages of RSV within the pediatric population in Senegal, especially in infants aged ≤ 11 months.

Groundwater is a major resource for drinking water, especially in developing countries, where it is less expensive to treat than surface water. Today, the resource is highly susceptible to pollution, particularly as a result of human activity. This review was based on a literature review and critical analysis of models for estimating the groundwater vulnerability. The results show that the deepest porous aquifers are the least susceptible to pollution, whereas those in karstic and fissured environments are susceptible, whatever their depth. Pollution usually arises from human activity. Critical analysis of the literature shows that existing methods are developed in specific environmental contexts. Given the variability of factors in space and time, these methods do not take the intrinsic realities of all natural settings into account adequately and are not perfectly applicable in all environments. This highlights the need to develop appropriate models for each environment, such as that of the highlands in countries such as Cameroon. HIGHLIGHTS The deepest porous aquifers are less susceptible to pollution than karstic and fissured aquifers.; Existing methods are developed in specific environmental contexts, to solve given problems.; The variability of factors in space and time means that pre-existing methods are not suitably valid in all natural settings.;

Objectives: Burnout syndrome (BOS) is an affection mostly resulting from chronic job-related stress. Many studies have identified job-related and non-job-related factors associated with BOS. Our aim of this study was to assess the level of BOS in private and public hospital healthcare providers in Fako division, Cameroon and evaluate the impact of physical activity and sleep quality (SQ). Methods: The study was carried out in five randomly selected hospitals in Fako Division over a three-month period. Consenting doctors, nurses and laboratory technicians were recruited using consecutive sampling methods. Sociodemographic and professional characteristics were collected using a structured questionnaire. BOS was assessed using the Maslach Burnout Inventory Human Services Survey (MBI-HSS). Sleep quality (SQ) and physical activity (PA) were assessed using the Pittsburgh Sleep Quality Index (PSQI) and Global Physical Activity Questionnaire (GPAQ) respectively. Odd ratios and 95% confidence intervals were calculated and a statistical significance was set for p-value < 0.05. Results: The mean age was 32 ± 6 years and 70.9% female. BOS prevalence was 66.3% with 71.4% in females and 53.9% in males (p = 0.002). Of the 232 participants with BOS, 65.7%, 52% and 53.7% had moderate to high emotional exhaustion, depersonalization and decreased personal accomplishment, respectively. Moderate to high PA as well as poor SQ were not significantly associated with BOS while longer sleep duration (>8 h) was associated with a greater odd of BOS. Conclusions: The prevalence of BOS was high among healthcare professionals. While PA showed no protective effects, high sleep duration could increase its risk.

Natural coagulants can be an alternative solution to minimise the environmental pollution and health risks caused by the use of chemical coagulants. The main objective of this research was to evaluate the performance of groundnut extract for turbidity removal from water. For this, the active coagulant agents were extracted from the deshelled nuts and used in a series of water treatment tests performed at low, medium, and high turbidity levels using synthetic turbid water. The groundnut extract showed good coagulative abilities, close to those of Al2(SO4)3. The best performance was obtained at medium turbidity (150 NTU), where the extract removed 89% of the turbidity using a 500 mg/L dosage. However, it was not as efficient as Al2(SO4)3, whose coagulation was better at every turbidity level. The use of the groundnut extract does not modify the water's pH significantly and the floc size decreases as turbidity increases, although they are bigger than those produced by Al2(SO4)3. Its flocculation and sedimentation processes are also quick (each less than 10 min) and quicker than those with Al2(SO4)3. Hence, the groundnut extract is a viable alternative to chemical coagulants. HIGHLIGHTS Groundnut extract can be used efficiently to treat water at any turbidity level.; Its application does not modify the pH of water significantly.; The treatment process is quick, less than 15 min.;

Abstract Background Living areas in developing countries impact seriously lifestyle by modifying energy consumption and energy expenditure. Thus, urbanization is associated with less practice of physical activity (PA), a leading cause of metabolic syndrome (MetS) which prevalence vary in African countries. The present study aimed to assess the effect of PA on MetS according to urbanization level in the littoral region, Cameroon. Methods A cross-sectional study was conducted in three geographical settings (urban, semi-urban, and rural) in the littoral region in Cameroon. A total of 879 participants were included (urban: 372, semi-urban: 195 and rural: 312). MetS was defined according to the International Federation of Diabetes 2009. The level of PA was assessed using the Global Physical Activity questionnaire. Results Low level of PA was (P

IntroductionCarbapenemase-Producing Escherichia coli (CP-Eco) isolates, though less prevalent than other CP-Enterobacterales, have the capacity to rapidly disseminate antibiotic resistance genes (ARGs) and cause serious difficult-to-treat infections. The aim of this study is phenotypically and genotypically characterizing CP-Eco isolates collected from Spain to better understand their resistance mechanisms and population structure.MethodsNinety representative isolates received from 2015 to 2020 from 25 provinces and 59 hospitals Spanish hospitals were included. Antibiotic susceptibility was determined according to EUCAST guidelines and whole-genome sequencing was performed. Antibiotic resistance and virulence-associated genes, phylogeny and population structure, and carbapenemase genes-carrying plasmids were analyzed.Results and discussionThe 90 CP-Eco isolates were highly polyclonal, where the most prevalent was ST131, detected in 14 (15.6%) of the isolates. The carbapenemase genes detected were blaOXA-48 (45.6%), blaVIM-1 (23.3%), blaNDM-1 (7.8%), blaKPC-3 (6.7%), and blaNDM-5 (6.7%). Forty (44.4%) were resistant to 6 or more antibiotic groups and the most active antibiotics were colistin (98.9%), plazomicin (92.2%) and cefiderocol (92.2%). Four of the seven cefiderocol-resistant isolates belonged to ST167 and six harbored blaNDM. Five of the plazomicin-resistant isolates harbored rmt. IncL plasmids were the most frequent (45.7%) and eight of these harbored blaVIM-1. blaOXA-48 was found in IncF plasmids in eight isolates. Metallo-β-lactamases were more frequent in isolates with resistance to six or more antibiotic groups, with their genes often present on the same plasmid/integron. ST131 isolates were associated with sat and pap virulence genes. This study highlights the genetic versatility of CP-Eco and its potential to disseminate ARGs and cause community and nosocomial infections.