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Dyes are generally defined as an aromatic organic compound which shows an affinity towards the substrate to which they are being applied. The presence of dyes in wastewater samples at any level is not safe for human. The presence of dyes in wastewater that being discharged from fabrics industry must be analyzed. Hence, a sensitive, precise, rapid, accurate, simple and low-cost analytical method is needed for dyes determination. The differential pulse anodic stripping voltammetric (DPASV) technique using glassy carbon electrode (GCE) as a working electrode and phosphate buffer at pH 4.2 as a supporting electrolyte has been proposed. The experimental voltammetric parameters were optimized in order to obtain a maximum response with analytical validation of the proposed technique. The optimum parameters were initial potential (Ei) = +0.3 V, end potential (Ef) = +1.0 V, scan rate (v) = 0.04 V/s, accumulation time (tacc) = 50 s, accumulation potential (Eacc) = 0.4 V and pulse amplitude = 0.075 V. The anodic peak was appeared at 0.77972 V. The curve was linear from 0.5 to 1.25 mg/L (R2=0.9986) with detection limit of 0.050 mg/L. The precisions in terms of relative standard deviation (RSD) were 0.08 %, 0.62 % and 0.50 % for consecutive three days. The range recovery achieved for 0.5, 0.7 and 1.0 mg/L of RB5 standard solution in simulation dye sample was 104.40 %, 89.71 %, and 111.15 %, respectively. It can be concluded that the proposed technique is precise, accurate, rugged, low cost, fast and has potential to be an alternative method for routine analysis of RB5. The proposed method will be tested for the content of RB5 dye in the real wastewater samples from fabric industry.