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This study evaluated the effect of roughage:concentrate (R:C) ratio associated with a variable particle size of physically effective neutral detergent fibre (peNDF8) in the forage (Tifton-85 hay) on the performance, carcass traits and meat quality of lambs. Seventy-two 4-month-old, non-castrated Santa Ines male lambs (23.5 ± 2.32 kg BW) were distributed in a completely randomized design, in a 2 × 2 factorial arrangement [two peNDF8 hay particle sizes (13 and 6 mm) and two R:C ratios (700:300 and 500:500 g/kg DM total)]. DMI, DM, NFC and TDN digestibility's, N-intake and N-faecal excretion were affected by the R:C ratio (P < 0.05). However, the N-retained was not affected by the studied variables (P > 0.05). It was observed an interaction (P < 0.05) between the peNDF8 and R:C ratios for final BW, average daily gain (ADG), colour parameters and pH 24 h. The lower roughage ratio provided greater (P < 0.05) concentrations of C14:1, C16:1–cis9, C18:1–cis9, ΣMUFA, Σn–6:Σn–3 and hypocholesterolemic/hypercholesterolemic index, enzymatic activity Δ9desaturase-C16 and -C18. Lambs fed a lower roughage diet had improved performance and feed efficiency, however, presented reduced polyunsaturated fatty acids (PUFA) concentrations in the meat, especially Σn–3 family. Higher roughage diet and larger peNDF8 particle size improved the concentrations of PUFA while decreased Σn–6:Σn–3 ratio in meat. Larger peNDF8 particle size associated with higher roughage proportion, have reduced animal performance however, it increased protein concentration, a* and C* colour parameter without affecting fatty acids profile of Longissimus lumborum muscle. [ABSTRACT FROM AUTHOR]

The current study was carried out to examine the effect of cottonseed processing and chitosan supplementation on lamb performance, digestibility and nitrogen digestion. Eighty uncastrated Santa Inês lambs (23 ± 2.2 kg average weight, 4 months old) were distributed in a completely randomized design in a 2 × 2 factorial arrangement that consisted of two cottonseed processing forms (whole or ground) and two chitosan levels (0 or 136 mg/kg live weight). Higher dry matter and organic matter apparent digestibility coefficient (ADC) was achieved with the diets containing the whole cottonseed. Ether extract ADC was higher in the animals fed the chitosan-containing diet. There was an interaction effect on the ADC of neutral detergent fibre corrected for ash and protein, which increased with chitosan inclusion associated with the whole cottonseed. The lambs that received the treatment containing the whole cottonseed showed higher microbial protein synthesis. Chitosan addition increased nitrogen retention. The animals fed chitosan-containing diets showed higher microbial protein synthesis. There was an interaction effect on microbial protein synthesis. Whole cottonseed associated with chitosan in lamb diets increases ether extract ADC and microbial protein synthesis. [ABSTRACT FROM AUTHOR]

Polymorphisms in the growth hormone (GH) and IGF type-1 (IGF1) genes have been associated with the economic traits in farm animals, including BW of some sheep breeds. However, it remains unknown if these polymorphisms also affect carcass traits in sheep. Thus, we aimed to identify polymorphisms in the GH and IGF1 genes in Santa Ines sheep in order to describe their allelic and genotypic frequencies as well as to test the hypotheses that they are associated with the carcass traits. Fragments of 4550 bp (IGF1) and 1194 bp (GH) were sequenced in up to 191 lambs. In all, 18 polymorphisms were identified in the IGF1 and 21 in the GH gene. The IGF1 polymorphisms rs430457475 , rs412470350 , rs409110739 and rs400113576 showed an additive effect on the internal carcass length (−0.9265±0.4223), rump girth (−2.9285±1.1473), rib yield (−1.0003±0.4588) and neck weight (−0.0567±0.0278), respectively. In addition, the polymorphisms rs58957314 in the GH affected the rib weight (−0.4380±0.1272) and rib yield (−2.2680±0.6970), loin weight (−0.1893±0.0516) and loin yield (−0.9423±0.3259), palette weight (−0.2265±0.0779) and palette yield (−0.9424±0.4184), leg weight (−0.3960±0.1375), neck weight (−0.0851±0.0394) and carcass finishing score (−0.1700±0.0839). These results allow us to conclude that there are polymorphisms in the IGF1 and GH genes associated with carcass traits in Santa Ines sheep, which can provide important information for marker-assisted selection. [ABSTRACT FROM AUTHOR]

Intake, digestibility, nitrogen (N) balance, microbial protein synthesis, weight gain, yields of the main commercial cuts and carcass morphometric measurements were evaluated in lambs fed diets containing different levels of chitosan. Sixty Santa Inês crossbred sheep with an average body weight (BW) of 24 ± 2.2 kg were assigned to three treatments (diets containing 0, 136 or 272 mg chitosan/kg BW) in a completely randomized design. There was no effect of chitosan on dry matter (DM) intake. Ingested and retained N showed a quadratic response, with the highest values estimated at the chitosan levels of 142 and 152 mg/kg BW, respectively. Similar to N balance, microbial protein synthesis showed the same quadratic response, in which the level of 136 mg/kg BW resulted in higher synthesis when compared with the other levels. No effect of chitosan was detected on average daily gain, final weight, or carcass variables (hot carcass weight, cold carcass weight, yield of commercial cuts and morphometric measurements of the carcass). Conformation, visceral fat content and fatness of carcasses were also not altered by the use of chitosan. Chitosan improves the digestibility of DM, crude protein and neutral detergent fibre, and increases N balance and microbial protein synthesis but does not change the production performance of feedlot lambs. [ABSTRACT FROM AUTHOR]

Alterations in progesterone (P4) catabolism due to high feed intake underlie some effects of nutrition on reproduction. Based on previous research, we hypothesized that high feed intake could potentially increase P4 catabolism, likely due to increased liver blood flow. However, there could also be an opposing action due to increased circulating insulin, which has been shown to inhibit hepatic expression of key enzymes involved in P4 catabolism. To test which effect would have the greatest impact on circulating P4 during a 1- and 2 -mo time frame, we used a noncyclic ewe model. The plane of nutrition was controlled, and effects on circulating insulin, P4 catabolism in response to exogenous P4, and steady state mRNA for key hepatic enzymes were evaluated. Twentyfour F1 Dorper × Santa Inês ewe lambs (5 mo old and approximately 25 kg BW) were used. After 14 d of adaptation, ewes were randomized into 2 groups: ad libitum fed (Ad), with intake of 3.8% DM/kg BW, or restricted feed intake (R), with 2% DM/kg BW, for 8 wk. At wk 4 and 8, ewes received an intravaginal P4 implant to evaluate P4 catabolism. As designed, Ad ewes had greater daily feed intake than R ewes (means of 1.8 [SE 0.03] and 0.6 kg/ewe [SE 0.01]; P < 0.001) and greater weekly gain in BW (means of 1.7 [SE 0.12] vs. -0.1 kg/ewe [SE 0.03]; P < 0.001). Mean circulating insulin of samples collected from -0.5 to 7 h after the start of feeding was over 5-fold greater in Ad ewes than in R ewes (least squares means of 8.2 [SE 0.93] vs. 1.5 μIU/mL [SE 0.16], respectively, at wk 4 and 12.0 [SE 1.02] vs. 2.2 μIU/mL [SE 0.18], respectively, at wk 8; P < 0.001). Although both groups received the same P4 treatment, mean circulating P4 of samples collected from -0.5 to 7 h after feeding was much lower in Ad ewes than in R ewes (least squares means of 3.2 [SE 0.32] vs. 5.5 ng/mL [SE 0.32], respectively, at wk 4 and 2.8 [SE 0.28] vs. 5.2 ng/mL [SE 0.28], respectively, at wk 8; P < 0.001) indicating much greater P4 catabolism in ewes with high feed intake. Unexpectedly, there was no effect of diet on hepatic mRNA concentrations for CYP2C, CYP3A, AKR1C, or AKR1D at wk 4 or 8 in spite of dramatically elevated insulin. Therefore, high energy/feed intake primarily increased P4 catabolism with no evidence for offsetting effects due to insulin-induced changes in hepatic P4 metabolizing enzymes. [ABSTRACT FROM AUTHOR]

The purpose of this study was to evaluate the effect of partial replacement of corn in the starter feed with crude glycerin on performance and energy metabolism of liquid-fed dairy calves. Twenty-four newborn individually housed Holstein calves were blocked according to weight and date of birth and randomly assigned to different starter feed treatments during 8 weeks: no crude glycerin; 5% crude glycerin; 10% crude glycerin on dry matter basis. All calves were individually fed daily 4 L of a commercial milk replacer (22.3% crude protein and 19.3% fat). Calves had individual access to starter diet and ad libitum access to water daily. Individual feed intake and faecal scores were recorded daily. Bodyweight and corporal measurements (heart girth, hip width, and withers height) were weekly recorded. Fromthe second weekof age, blood samples were collected weekly, 2 h after morning feeding, for determination of plasma glucose and b-hydroxybutyrate concentrations. Ruminal fluid was collected at 4, 6 and 8 weeks of age for determination of pH and short-chain fatty acids. At the end of the eighth week, animals were harvested to evaluate development of the proximal digestive tract. Feed intake, average daily gain, bodyweight or body measurements were not affected (P > 0.05) by the inclusion of crude glycerin in the starter feed. Faecal scores indicated that most of the calves had mild diarrhoea, with no treatment effect. Total shortchain fatty acids and the concentration of individual acids, as well as blood parameters, were not affected (P > 0.05) by the feed treatments. The total forestomach weight, the average weight of each compartment, themaximum capacity of reticulumrumen, and papillae number, height and width were not affected (P > 0.05). In conclusion, crude glycerin can be used as an alternative energy source, replacing corn up to 10% in the starter feed for liquid-fed dairy calves without affecting growth, rumen development or metabolism. [ABSTRACT FROM AUTHOR]

Copyright of Archivos de Zootecnia is the property of Archivos de Zootecnia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study screened for asymptomatic primary hyperparathyroidism (PHPT) by measuring calcium (Ca) before thyroid surgery. The study was prospective. A total of 676 patients without a suspicion of PHPT were studied. PHPT was defined as elevated Ca (in 2 measurements) in the presence of PTH > 25 pg/ml and in the absence of hypocalciuria. PHPT was diagnosed in 5 patients (0.74 %), all of them women. One of the 5 patients with PHPT had no indication for parathyroidectomy (PTx) according to current guidelines. Parathyroid adenoma was easily identified during perioperative assessment of thyroid surgery in 3 patients. In the other 2 patients (0.3 %), localization of the adenoma required specific exploration, which was only performed because of the preoperative diagnosis of PHPT. Normalization of Ca and PTH was achieved in all 5 patients. In conclusion, in patients without a clinical or ultrasonographic suspicion of PHPT, approximately 300 individuals should be screened before thyroid surgery to avoid one reoperation (PTx) due to PHPT, which does not seem to be cost-effective. [ABSTRACT FROM AUTHOR]

The myogenic differentiation 1 gene ( MYOD1) has a key role in skeletal muscle differentiation and composition through its regulation of the expression of several muscle-specific genes. We first used a general linear mixed model approach to evaluate the association of MYOD1 expression levels on individual beef tenderness phenotypes. MYOD1 mRNA levels measured by quantitative polymerase chain reactions in 136 Nelore steers were significantly associated ( P ≤ 0.01) with Warner-Bratzler shear force, measured on the longissimus dorsi muscle after 7 and 14 days of beef aging. Transcript abundance for the muscle regulatory gene MYOD1 was lower in animals with more tender beef. We also performed a co-expression network analysis using whole transcriptome sequence data generated from 30 samples of longissimus muscle tissue to identify genes that are potentially regulated by MYOD1. The effect of MYOD1 gene expression on beef tenderness may emerge from its function as an activator of muscle-specific gene transcription such as for the serum response factor (C-fos serum response element-binding transcription factor) gene ( SRF), which determines muscle tissue development, composition, growth and maturation. [ABSTRACT FROM AUTHOR]

The effect of tumbling with brines on different portions of the biceps femoris muscle was evaluated for the quality of beef from Bos indicus cattle older than 30 mo. Six biceps femoris muscles were divided into portions: origin (OP), insertion 1(IP1), and insertion 2 (IP2). The portions were sliced into steaks and were treated with no tumbling (control), tumbling with brine (BR), and tumbling with brine and hydrolyzed soy protein (BR+HSP). The steaks were vacuum packaged and stored for 1 and 12 d and then analyzed for pH, yield, color, cooking loss, and shear force. The control steaks from the OP had higher (P < 0.05) pH and shear force values and lower (P < 0.05) L* values than the control steaks from IP2. The pH and a* and b* values increased (P < 0.05) and the L* values and cooking loss decreased (P < 0.05) when the steaks were tumbled with BR and BR+HSP. Overall, substantial variation was found for the variables among the different portions of the biceps femoris muscle. Based on the lower lightness (low exudation) and greater yellow and red intensity (less oxidation) of the meat, the tumbling with brines improved the meat quality. However, the hydrolyzed soy protein incorporated into the brine did not increase the effect compared with using the brine alone for most of the variables. [ABSTRACT FROM AUTHOR]

Camera traps were set up in forest nests in 2009, 2010 and 2012 to capture images of possible predators eating eggs of the Pantanal caiman,Caiman crocodilus yacare. We monitored 57 caiman nests; 42 nests were opened and the eggs were counted (mean = 25 eggs/nest, SD = 4.3). Females were present and captured at 38 of those nests. The remaining 15 nests were used as controls, and we did not capture the females or open the egg cavities of these nests. Most of the nests had the eggs eaten by predators, in both the disturbed group (38 nests) and the control group (13 nests). The main predators were carnivorous mammals, such as crab-eating foxes (Cerdocyon thous), coatis (Nasua nasua) and tayras (Eira barbara), although feral pigs (Sus scrofa) and armadillos (Dasypus novemcinctus) were also photographed eating caiman eggs. The lizardSalvator merianaewas photographed eating eggs of two nests. [ABSTRACT FROM PUBLISHER]

The inclusion of genetic groups in sire evaluation has been widely used to represent genetic differences among animals not accounted for by the absence of parentage data. However, the definition of these groups is still arbitrary, and studies assessing the effects of genetic grouping strategies on the selection efficiency are rare. Therefore, the aim in this study was to compare genetic grouping strategies for animals with unknown parentage in prediction of breeding values (EBV). The total of 179,302 records of weaning weight (WW), 29,825 records of scrotal circumference (SC), and 70,302 records of muscling score (MUSC) from Montana Tropical animals, a Brazilian composite beef cattle population, were used. Genetic grouping strategies involving year of birth, sex of the unknown parent, birth farm, breed composition, and their combinations were evaluated. Estimated breeding values were predicted for each approach simulating a loss of genealogy data. Thereafter, these EBV were compared to those obtained in an analysis involving a real relationship matrix to estimate selection efficiency and correlations between EBV and animal rankings. The analysis model included the fixed effects of contemporary groups and class of the dam age at calving, the covariates of additive and nonadditive genetic effects, and age, and the additive genetic effect of animal as random effects. A second model also included the fixed effects of genetic group. The use of genetic groups resulted in means of selection efficiency and correlation of 70.4 to 97.1% and 0.51 to 0.94 for WW, 85.8 to 98.8% and 0.82 to 0.98 for SC, and 85.1 to 98.6% and 0.74 to 0.97 for MUSC, respectively. High selection efficiencies were observed for year of birth and breed composition strategies. The maximum absolute difference in annual genetic gain estimated through the use of complete genealogy and genetic groups were 0.38 kg for WW, 0.02 cm for SC, and 0.01 for MUSC, with lower differences obtained when year of birth was adopted as a genetic group criterion. Grouping strategy must consider selection decisions and the number of genetic groups formed, in the way that genetic groups represent the genetic differences in population and allow an adequate prediction of EBV. [ABSTRACT FROM AUTHOR]

Copyright of Archivos de Zootecnia is the property of Archivos de Zootecnia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The giant otter (Pteronura brasiliensis) is a social species that defends territories along water bodies. Although some researchers have visually monitored otters during long periods at night, no nocturnal activity of the species has been recorded and giant otters are currently believed to be strictly diurnal. In this study, we present information about the activity patterns of groups of giant otters in the Brazilian Pantanal, using radio telemetry and camera trap data. We captured, implanted transmitters in, and monitored three male giant otters from different groups in the Miranda and the Vermelho Rivers between November 2009 and June 2011. The locations and behavior of the group were recorded at 30-min intervals from 05:00 to 19:00. Camera traps were positioned at the active dens and latrines of eight groups of giant otters in the Miranda, Vermelho and Negro Rivers between June 2010 and October 2011. The groups of giant otters were mostly crepuscular and diurnal, but 31% of the camera-trap recordings were nocturnal. Fishing was the most frequent (64%) behavior recorded by telemetry. Giant otters were recorded exiting the den mostly in the early morning (06:00) and entering the den at the end of the day (16:00 to 19:00). Nocturnal activity appeared to be associated with the need to defecate, prey availability nearby the den and predation risk. [ABSTRACT FROM AUTHOR]

Meat quality traits are economically important because they affect consumers' acceptance, which, in turn, influences the demand for beef. However, selection to improve meat quality is limited by the small numbers of animals on which meat tenderness can be evaluated due to the cost of performing shear force analysis and the resultant damage to the carcass. Genome wide-association studies for Warner-Bratzler shear force measured at different times of meat aging, backfat thickness, ribeye muscle area, scanning parameters [lightness, redness (a*), and yellowness] to ascertain color characteristics of meat and fat, water-holding capacity, cooking loss (CL), and muscle pH were conducted using genotype data from the Illumina BovineHD BeadChip array to identify quantitative trait loci (QTL) in all phenotyped Nelore cattle. Phenotype count for these animals ranged from 430 to 536 across traits. Meat quality traits in Nelore are controlled by numerous QTL of small effect, except for a small number of large-effect QTL identified for a*fat, CL, and pH. Genomic regions harboring these QTL and the pathways in which the genes from these regions act appear to differ from those identified in taurine cattle for meat quality traits. These results will guide future QTL mapping studies and the development of models for the prediction of genetic merit to implement genomic selection for meat quality in Nelore cattle. [ABSTRACT FROM AUTHOR]

The purpose of this study was to correlate the activity and habitat use of a large and ecologically specialized mammal ( Myrmecophaga tridactyla) with the time of day and minimum daily temperature in Brazil's Pantanal wetland. Seven giant anteaters were fitted with very high frequency (VHF) radio transmitters and monitored from March to December 2001. The animal's state of activity or rest, time and ambient temperature were recorded in different patches of the study site, which were covered by a mosaic of vegetation types and several ponds. Whenever a giant anteater was inside a forest patch, the temperature inside and outside of the patch was also recorded. The temperatures throughout the day were recorded by the meteorological station at the study site. The period of greatest activity of giant anteaters started at 18:00 h on hot days, but whenever the daily minimum temperature declined, the species' activity began earlier. Giant anteaters use predominantly forest habitats for rest and open habitats for activity. The forest patches work as a temperature buffer, because they are cooler than the matrix of open habitats during the hot hours of the day and warmer than the matrix during the cold hours. Although the giant anteater is known to be associated with open habitats, this study indicates the need of preserving forest patches for the conservation of this species. [ABSTRACT FROM AUTHOR]

Eight patients with life-threatening hemobilia were treated by percutaneous transcatheter occlusive therapy. The bleeding was caused by a traumatic pseudoaneurysm of the hepatic artery in 6 cases (auto accident in 4, surgery in 1, biliary drainage in 1) and a true aneurysm of the hepatic artery in 2 (unknown etiology in 1 and mycotic in 1). Arterial catheterization was used in all cases except for one in which a direct percutaneous puncture was performed. Gelfoam alone was used as embolic material in 3 patients. In 1 patient each, the material used was gelfoam plus coils, coils alone, blood clot, n-butyl-cyanoacrylate and an occluding balloon catheter. In all cases the bleeding stopped and did not recur during the follow-up period which ranged from 9 months to 14 years. This experience indicates that transcatheter occlusive therapy is an effective method for the treatment of severe hemobilia. [ABSTRACT FROM AUTHOR]

Intraarterial injection of Lipiodol has been recommended to differentiate hepatocellular carcinoma from benign lesions such as cavernous hemangioma, because uptake and prolonged retention of the contrast medium is a characteristic of the malignant tumors. In two cases of cavernous hemangioma of the liver in which we injected Lipiodol, uptake and retention up to 3 months was demonstrated. We conclude that the intraarterial injection of Lipiodol may not be reliable in differentiating hepatocellular carcinoma from cavernous hemangioma of the liver. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the occurrence of differences between genetic groups of buffaloes for milk production traits, as total milk yield (TM/kg), protein (Prot/kg), fat (Fat/Kg), lactose (Lact/kg) and total solids (TS/kg), and estimate from them heterosis for TM/kg. To this aim, we used 609 observations belonging to 248 lactations of Murrah (Mu) and Mediterranean buffaloes in the different breed proportions, ½Murrah + ½Mediterranean (½Mu½Me) and ¾Murrah x ¼ Mediterranean (¾Mu¼Me) of from the Rio Pardo's farm, localized in Bocaina city (SP-Brazil). The variance analysis were performed by the methodology of mixed models, considering as fixed effects genetic group and the contemporary groups, constituted for year. The calving age of dams (linear and quadratic effects) and the lactation length (linear effect) was considering as covariates of the model. The animal was considering as random effects. The genetic group had a significant effect (P<0,05) for all traits, except Lac/kg. The mean value for MT/kg were 1947 ± 23.268kg; 2157.4 ± 45.6kg; 2078.6 ± 73.9kg and 2015 ± 63.8kg for Mu, ½Mu½Me, ¾Mu¼Me and Me buffaloes respectively, differences were not statistically significant, except between the means of animals Mu and ½Mu½Me (P<0.05). For the constituents of milk, the estimated average protein, fat and total solids were, respectively, equal to 85.0±1.8Kg, 124.4±3.4Kg and 331.4±6.9kg for Mu; 97.4±2.9Kg, 139 .9±5.3kg e 347.7±6.9kg for ½ Mu½Me (F1); 98.7±5.9kg, 123.7±10.9kg and 374.6±22.1kg for ¾Mu¼Me; e 97.6±4.7kg, 148.1±8.5kg e379.9±17.4kg for Me buffaloes. The estimative of heterosis in F1 (½Mu½Me) was 8.9% to TM/kg, while for animals ¾Mu¼Me was 4.9%. These results shows that the group F1 with the maximum expected heterosis (100%) has the highest TM/kg, according to the assumption that increasing heterozygosity lead to augment heterosis, whilst the retention of heterosis in animals ¾Mu¼Me is 50%. It should be noted, however, that further studies on the performance of crossbred animals are needed, considering, particularly a greater number of observation, beyond the effect of addition of breed. [ABSTRACT FROM AUTHOR]

Embolization therapy is reported in 2 patients who had vascular complications following percutaneous subclavian vein catheterization. One had an arteriovenous fistula between the right internal mammary artery (IMA) and the brachiocephalic vein. The other patient presented with a pseudo-aneurysm of the IMA with life-threatening hemorrhage and a large thoracic hematoma. A detachable latex balloon was used for occlusion of the arteriovenous fistula in the first patient and a steel spring coil was used to embolize the IMA and the pseudo-aneurysm in the second patient. The lesions were successfully treated on follow-up of 30 months for the first patient and 37 months for the other. [ABSTRACT FROM AUTHOR]

The pampas deer is an endangered South American species which occurs in open grasslands and savannas. This aim of this survey was to evaluate the use of the distance sampling technique to estimate densities of the species in the Pantanal wetland, as well as to analyze the applicability of the method for a monitoring program. The surveys were conducted on roads from vehicles and also on foot along 26 parallel transects in November 1999 and 2000 at Campo Dora ranch, south-central Pantanal, Brazil. Deer densities were estimated using the program DISTANCE, and the program MONITOR was used to run a power analysis to estimate the probability of detection of a decline in the population. The deer density estimated from vehicles, with data from both years, was 9.81±3.8 individual/km2, and 5.53±0.68 individuals/km2 from transects sampled on foot. The power analysis of these data revealed a monitoring program would require at least two surveys per year over seven years to obtain a 90% chance of detecting a 5% decline in the population. Our results also indicate surveys from roads are not recommended for pampas deer counts as the animals appear to keep a relatively safe distance from cars.

Polyunsaturated fatty acids seem to exert an extra-caloric positive effect on ruminant reproduction, although the reasons for that are still unclear. Although some studies have detected a positive effect of feeding unsaturated fatty acids on embryo development of superovulated Bos tauruscattle (Thangavelu et al.2007 Theriogenology 68, 949-957), others have not (Petit et al.2008 J. Dairy Sci. 91, 1786-1790). Our hypothesis was that, although number and quality of embryos from superovulated heifers would not be affected by diet, supplemental fat would improve embryo cryotolerance. Therefore, this study evaluated superovulatory response and embryo production, as well as cryotolerance of embryos cryopreserved through freezing or vitrification in Nellore heifers supplemented with rumen-protected fat. Forty heifers (24 to 36 mo old) were kept in pasture and randomly divided into 2 experimental groups according to supplemental source [F = concentrate with rumen-protected fat (100 g/d of Megalac-E®) and C = control, without fat supplementation]. Supplements were formulated to be isocaloric and isoproteic. Each female underwent both treatments in a cross-over design with approximately 68 d between replicates. After 50 d of feeding, emergence of the wave was synchronized with the aid of hormones to initiate the superovulation protocol. Recovered embryos were frozen or vitrified, and subsequently in vitroembryo development evaluation was accomplished. Data were analyzed using generalized linear models. There was no difference between F and C groups (P> 0.10) regarding superstimulatory response, number of total embryos/ova, viable embryos, degenerate embryos, or unfertilized oocytes recovered. However, group C had a greater superovulatory response than F (18.0 ± 1.3 v.15.7 ± 1.2 CL; P= 0.06). Group C embryos presented greater hatching rate, independently of the cryopreservation method, at 48 h (33.1 ± 4.0%; n= 148 v.17.3 ± 3.3%; n= 137; P= 0.009) and at 72 h (44.3 ± 4.2%; n= 148 v.30.9 ± 4.0%; n= 137; P= 0.04) of in vitroculture. Under the conditions of the present study, supplementation with protected fat did not affect superstimulatory response and quantity or quality of embryos. However embryos from the F group were less tolerant to cryopreservation. Financial support from FAPESP, EMBRAPA, Arm & Hammer, Integral Produbon, and Pfizer of Brazil. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the influence of high or low dry matter (DM) intake and/or energy on embryo quality and production in vivo. Nonlactating Nelore cows (n = 32, 4 to 10 years old) weighing 489.5 ± 11.3 kg and with a body condition score of 3.25 (1 to 5) were used. After 15 days on the adaptation diet, cows were blocked by initial body weight (BW) and randomly divided in 4 experimental groups. The maintenance group (M) received a diet to provide 1.2% of DM/kg of BW. The restriction group (0.7M) received the equivalent of 70% of the group M diet (0.84% of DM per kg of BW). The high intake group (1.5M) received the equivalent of 150% of the M group (1.8% of DM/kg of BW). The energy group (E) received a diet with a DM similar to the M group but with an energy level equivalent to the 1.5 M group. All cows were submitted to aspiration of follicles >2 mm for ovum pick-up (OPU). Recovered oocytes were used in another experiment. The embryo donors received an intravaginal device (IVD) of progesterone release (Sincrogest®, Ouro Fino, Ribeirão Preto, Brazil), soon after OPU. Two days after OPU, the cows received 8 decreasing doses of FSH (100 mg, IM, Folltropin-V®, Bioniche Animal Health, Belleville, Ontario, Canada) and concomitant with the fifth and sixth treatments of FSH, PGF2α (500 μg each, IM, cloprostenol, Sincrocio®, Ouro Fino) was administered. The IVD was removed at the time of the last FSH injection. Twelve hours after IVD removal ovulation was induced with GnRH (0.01 mg, IM, Buserelin acetate, Sincroforte®, Ouro Fino). Cows were inseminated 12 and 24 h later. Embryo collection was performed 7 or 8 days after GnRH injection. The cows were offered all diets in a crossover design study. There were 4 sessions of embryos flushing, each 42 days apart. Data were analysed by PROC GLIMMIX of SAS (SAS Institute Inc., Cary, NC, USA) and the results are presented as least-squares means ± SE, always following the order of treatments 0.7 M, M, 1.5 M and E. There were differences in the superstimulatory response (12.6 ± 1.4; 14.6 ± 1.6; 13.6 ± 1.5ab; and 11.0 ± 1.2 follicles >6 mm; P < 0.01) and superovulatory response (9.8 ± 1.3ab; 11.0 ± 1.4; 10.2 ± 1.3; and 8.6 ± 1.3 CL; P < 0.01) among groups. Despite the lower responses observed especially in the high-energy group, no difference among groups was observed for total embryos/ova (4.5 ± 0.7; 5.0 ± 0.8; 5.0 ± 0.8; and 4.7 ± 0.7; P = 0.60), viable embryos (2.0 ± 0.4; 2.3 ± 0.5; 2.6 ± 0.6; and 2.2 ± 0.5; P = 0.40), or freezable embryos (1.7 ± 0.4; 2.0 ± 0.4; 2.1 ± 0.5; and 1.9 ± 0.4; P = 0.60). There was also no difference among groups for fertilization rate (75.8 ± 9.6; 82.3 ± 8.0; 87.8 ± 6.6; and 81.1 ± 8.4%; P = 0.71) and percentage of viable embryos (54.5 ± 10.8; 50.8 ± 10.6; 50.5 ± 10.9; and 54.4 ± 10.7%; P = 0.98). In conclusion, in contrast to our initial hypothesis, a period of 42 days under high feed/energy intake in nonlactating zebu cows apparently did not compromise in vivo embryo production. This may be because cows had a moderate body condition score or because the feeding period was not long enough to compromise oocyte/embryo quality. Financial support from FAPESP and CNPq is acknowledged. [ABSTRACT FROM AUTHOR]

Recent studies that have used eCG administration either 14 or 22 days after fixed-time AI in dairy and beef cattle have indicated a positive effect on conception rates. The aim of this study was to evaluate conception rates in embryo recipients treated with eCG 7 days after fixed-time embryo transfer (ET). A protocol for synchronization of ovulation was used in 470 Nelore and crossbred embryo recipient heifers (n=179), dry cows (n=152), and lactating cows (n=139; body condition score=3.0 to 3.5; >40 days in milk). All heifers and dry cows, but only 27.3% of lactating cows, were cycling at the beginning of the synchronization protocol. On Day 0, each female received IM injections of 2mg of oestradiol benzoate (Gonadiol, Intervet Schering-Plough Animal Health, São Paulo, Brazil) and 0.075mg of PGF2α(Preloban, Intervet Schering-Plough Animal Health). An intravaginal progesterone-releasing device (DIB, Intervet Schering-Plough Animal Health) was also inserted on Day 0. On Day 8, the DIB device was removed and cows or heifers received another IM treatment of 0.075mg of PGF2α(Preloban), 300 IU of eCG (Novormon, Syntex S.A., Buenos Aires, Argentina) and 0.6mg of oestradiol cipionate (Pfizer, Brazil). Transrectal ultrasonography was performed in all recipients on the day of ET (Day 16, 17, or 18 of the protocol) and only those with a corpus luteum greater than 15mm in diameter were selected (n=113 heifers, 114 dry cows, and 70 lactating cows). Transfers of fresh, frozen, and vitrified in vivo-produced embryos from Nelore donors were balanced between experimental groups. Seven days after ET, 127 animals received an IM injection of 400 IU of eCG (eCG group), and the 170 remaining cows or heifers were not treated with eCG, and were therefore considered the control group. Pregnancy diagnosis was performed 30 and 60 days after ovulation by transrectal ultrasound. Data were analysed using generalized linear models and results are presented as least squares means±standard error. Conception rates were similar in the eCG and control groups at Day 30 (35.9±5.5 and 33.5±4.4, respectively; P>0.10) and at Day 60 (27.6±4.9 and 26.7±3.9, respectively; P>0.10). Contrary to other results in the literature, especially in dairy cows bred by fixed-time AI, the use of eCG 7 days after ET did not improve conception rates in Bos indicuscattle used as embryo recipients. Financial support from CNPq, FAPESP, and Intervet Schering-Plough Animal Health of Brazil and Syntex S.A. of Argentina. [ABSTRACT FROM AUTHOR]

Results from a previous experiment (Barros C et al.2008 Reprod. Fertil. Dev. 20, 152 abst) have shown that the replacement of porcine FSH (pFHS) by eCG on the last day of the superstimulatory treatment in Nellore (Bos indicus) cows resulted in a greater superovulatory (SOV) response as compared with treatment exclusively with pFSH. However, another study (Sartori R et al.2009 Reprod. Fertil. Dev. 21, 245-246 abst) in Nellore heifers did not corroborate those observations. The aim of this study was to evaluate the SOV response of Sindi (Bos indicus) donors submitted to this protocol. Forty-eight SOV treatments were performed in a crossover design in 19 nulliparous and primiparous females that were randomly divided into 2 groups: FSH (n= 24), which consisted of 8 pFSH injections (Folltropin®-V, Bioniche Animal Health; Belleville, Ontario, Canada), or FSH-eCG (n= 24), which consisted of 6 pFSH injections followed by 2 eCG injections (Folligon®, Intervet Schering-Plough Animal Health, Brazil). Each female underwent 2 or 3 SOV treatments that consisted of an i.m. injection of 2 mg of estradiol benzoate (Gonadiol®, Intervet) and the insertion of an intravaginal progesterone-releasing device (DIB®, Intervet) on Day 0. On Day 4, the superstimulatory treatments (100 or 150 mg of pFSH, based on previous history of SOV responses) were initiated and given in decreasing doses twice a day over a 4-day period. In the FSH-eCG group, the last 2 doses of pFSH were replaced by 2 doses of eCG (150 or 200 IU of eCG each). At the time of the fifth and sixth injections of FSH, 0.150 mg of PGF2 (Preloban®, Intervet) was injected i.m. The DIB®device was removed at the time of the last FSH or eCG injection and ovulation was induced with 0.2 mg of GnRH (Fertagyl®, Intervet) 12 h later. All females were artificially inseminated with frozen-thawed semen from the same bull 12 and 24 h after GnRH treatment. Seven days after the first AI, embryos/ova were recovered. Superstimulatory (number of follicles ≥6 mm at the time of the last FSH or eCG injection) and SOV (CL number) responses were determined by transrectal ultrasonography. Data were analyzed using generalized linear models and results were presented as least squares means ± standard error. The FSH-eCG group had higher superstimulatory (33.3 ± 3.9 v. 24.2 ± 3.0 follicles; P≤0.06) and SOV (16.8 ± 2.9 v. 10.8 ± 2.1 CL; P≤ 0.10) responses. Although the number of total ova/embryos recovered was not different between groups (8.2 ± 1.8 v. 5.9 ± 1.4 for FSH-eCG and FSH groups, respectively; P> 0.25), the number (6.5 ± 1.2 v. 2.4 ± 0.7; P≤ 0.008) and percentage (75.2 ± 6.6 v. 52.8 ± 8.6%; P≤ 0.05) of viable embryos was greater for the FSH-eCG females. Contrasting with the results in Nellore heifers, this study observed improvement in superstimulatory and SOV responses and embryo quality by replacing the last 2 doses of pFSH by eCG in Sindi donors. Financial support from FAPESP, EMBRAPA, Intervet Schering-Plough Animal Health, Nutricell, and Sindi do ACS of Brazil. [ABSTRACT FROM AUTHOR]

Several studies using sex-sorted sperm by flow cytometry have shown that its fertility is reduced. Therefore, this study evaluated structural and functional characteristics of sperm sexed by flow cytometry. In addition, in vitroembryo production (IVP) and development was assessed when frozen-thawed unsorted and sex-sorted sperm from 4 Nellore bulls. Each ejaculate was separated into three fractions: non-sexed (NS), sexed for X-sperm (SX), and sexed for Y-sperm (SY). After thawing, each sample was analyzed for sperm motility by computer-assisted semen analysis (CASA, Berkeley, CA), sperm head agglutination, sperm morphology, membrane integrity by propidium iodide (PI) and 6-carboxy-fluorescein diacetate (CFDA) staining, acrosome integrity by peanut agglutinin (PNA), capacitation by chlortetracycline (CTC), and chromatin integrity by acridine orange staining. Then, the samples were placed in 45 : 90% (NS90) or 45 : 60% (NS60, SX, and SY) Percoll™ gradients. After Percoll™ centrifugation, sperm pellets were analyzed or used for IVP. All analyses were replicated independently three times. For IVP, 2,271 in vitromatured oocytes were used. To assess fertilization rate, presumptive zygotes were fixed and stained with lacmoid at 18 h post-insemination (hpi). Cleavage was evaluated at Day 2 (48 hpi) and blastocyst development at Days 6, 7, 8, and 9 of culture. Data were analyzed using generalized linear models. No differences (P> 0.05) were observed between SX and SY groups for e sperm variables evaluated either before or after Percoll™. However, non-sexed sperm had higher sperm motility, greater percentage of sperm with intact membranes, and greater percentage of live sperm with intact acrosomes than sexed sperm (P< 0.05). An effect of Percoll™ was observed in the non-sexed samples, with those submitted to 45 : 90% gradient having higher motility, greater percentage of cells with intact membrane, and lower recovery rate than those submitted to a 45 : 60% gradient. No differences among groups were observed for fertilization rate, being 74.0 ± 5.7, 63.2 ± 5.1, 67.2 ± 5.7, and 55.4 ± 5.9% for NS90, NS60, SX, and SY, respectively. Group NS90 showed a greater cleavage rate than did the SY group, while groups NS60 and SX had similar rates to the others. Blastocyst development rates on Day 6 to Day 9 were greater for group NS90. For example, on Day 8 the blastocyst rate was 34.9 ± 3.6, 22.2 ± 3.2, 18.1 ± 3.3, and 14.8 ± 2.9% forNS90, NS60, SX, and SY groups, respectively. All groups showed similar embryonic developmental stages on Day 6 to Day 9. Although sex-sorting affected sperm characteristics, it did not cause a decrease with in vitrofertility. However, differences in blastocyst rates between groups NS60 and NS90 indicated that the sperm selection protocol affected embryo production. Financial support: Embrapa Genetic Resources and Biotechnology. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the effects of different liquid-feeding systems using a medium crude protein milk replacer on performance, rumen, and blood parameters. Thirty newborn Holstein calves were blocked according to birth weight and date of birth, and randomly distributed to different liquid-feeding systems: conventional (4 L/d), intensive (8 L/d), or stepup/step-down (wk 1, 4 L/d; wk 2 to 6, 8 L/d; wk 7 and 8, 4 L/d). The commercial milk replacer (12.5% solids, 20.2% crude protein, 15.6% fat) was fed twice daily (0700 and 1700 h) until calves were weaned, at 8 wk of age. Calves were individually housed in wood hutches, with free access to water and starter concentrate, and to hay only after weaning. They were followed through 10 wk of age. Milk replacer and starter intake were inversely affected by feeding system. After weaning, starter intake and hay intake were similar among feeding systems. Total dry matter intake was higher during the liquid-feeding period for calves on the intensive system compared to calves on the conventional system, but conventional feeding resulted in the highest dry matter intake after weaning. Feed efficiency was similar among feeding systems before and after weaning. Average body weight and daily gain were not affected by feeding system before or after weaning. During liquid feeding, diarrhea occurrence was lower for calves on the conventional system; however, when calves on the step-up/step-down system were fed lower volumes of liquid feed, diarrhea occurrence was similar to that of calves on the conventional system. Plasma concentrations of β-hydroxybutyrate were higher for calves on the conventional system, reflecting starter intake. Rumen pH, short-chain fatty acids, and N-NH3 were not affected by feeding system. Feeding higher volumes of milk replacer with a medium crude protein content had no beneficial effect on the performance of calves up to 10 wk of age. [ABSTRACT FROM AUTHOR]

The resurgent navigation project known as the Hidrovia Paraguay-Paraná threatens the integrity of the Pantanal, the world's largest wetland, which is considered a biome of its own. Intensive barge navigation is intended to transport crops (soybean, sugar, corn) and cement, iron and manganese from areas of production in Brazil, Paraguay and Bolivia to the oceanic ports of the Plata River. This short communication assembles the information available on the potential impacts of the planned deepening of the natural channel of the Paraguay River in its ~700 km-long upper section. These river channel alterations would disconnect the river from its floodplain, shorten the inundation period, and shrink the wetland area, resulting in severe degradation of the globally outstanding biological and cultural diversity of the Pantanal. The river sediments are mostly sandy and would require perpetual dredging. The reaches needing the most intensive dredging are those of the highest ecological value, protected as a National Park, UNESCO World Heritage and Biosphere Reserves, and various Indigenous reserves and Ramsar sites. Climate change is projected to increase the occurrence of low water periods. Between 2019 and 2021, navigation was impossible even in the already-deepened reaches of the Paraguay River between Corumbá and Asuncion during long periods of the year. Thus, despite considerable financial and technical efforts, the success of the navigation project is doubtful, whereas enormous environmental, cultural, and social impacts can be anticipated. For these reasons, the Brazilian government had already turned down the project in 2000. We suggest alternative, less impactful modes of transport of commodities, e.g., via railway., (Copyright © 2023. Published by Elsevier B.V.)

The giant otter (Pteronura brasiliensis) is a semiaquatic carnivore and a top predator in the trophic chain, considered a sentinel of freshwater ecosystems. Ticks are common ectoparasites of worldwide distribution and potential vectors of diseases. In this study, we report the ectoparasitism by ticks on a giant otter carcass found during monitoring activity at the Negro River, that holds a viable population of this endangered species in the Brazilian Pantanal. A total of three tick specimens were collected: two adults were identified as Amblyomma sculptum and a nymph as Amblyomma spp. There is a lack of information about the health of free-ranging giant otters and this report contributes to elucidate some of the host-parasite relationships, although much more research is needed to expand the knowledge about which kinds of pathogens are circulating in the species, especially among those transmitted by ticks.

Giant otters are territorial semi-aquatic mammals. It is common to find several individuals exhibiting wounds and scars due to intraspecific conflicts. Myiasis is a parasitic infestation on living tissues of vertebrates caused by dipterous larvae, that usually develops in freshly open wounds and can seriously threaten the host's health. Ectoparasites seem to be rare among giant otters and myiasis had not been recorded in this species until now. Here, is presented one record of myiasis in a free-ranging giant otter found dead in the Pantanal, Brazil. An ulcerative lesion was found in the frontoparietal region, from which 22 larvae were recovered and identified as Cochliomyia hominivorax. The low occurrence of ectoparasites in giant otters might reflect their semi-aquatic habits and their grooming behavior, which makes it difficult for parasites to remain on the skin. The injured otter probably got the larvae after an intraspecific fight. Agonistic encounters between groups of giant otters have been reported before and these fights can result in serious wounds or even death. It was hypothesized that the myiasis caused by C. hominivorax deteriorated the health of the infested giant otter, which prevented recovery and accelerated its death.

Aim: Macroecological studies that require habitat suitability data for many species often derive this information from expert opinion. However, expert-based information is inherently subjective and thus prone to errors. The increasing availability of GPS tracking data offers opportunities to evaluate and supplement expert-based information with detailed empirical evidence. Here, we compared expert-based habitat suitability information from the International Union for Conservation of Nature (IUCN) with habitat suitability information derived from GPS-tracking data of 1,498 individuals from 49 mammal species., Location: Worldwide., Time Period: 1998-2021., Major Taxa Studied: Forty-nine terrestrial mammal species., Methods: Using GPS data, we estimated two measures of habitat suitability for each individual animal: proportional habitat use (proportion of GPS locations within a habitat type), and selection ratio (habitat use relative to its availability). For each individual we then evaluated whether the GPS-based habitat suitability measures were in agreement with the IUCN data. To that end, we calculated the probability that the ranking of empirical habitat suitability measures was in agreement with IUCN's classification into suitable, marginal and unsuitable habitat types., Results: IUCN habitat suitability data were in accordance with the GPS data (> 95% probability of agreement) for 33 out of 49 species based on proportional habitat use estimates and for 25 out of 49 species based on selection ratios. In addition, 37 and 34 species had a > 50% probability of agreement based on proportional habitat use and selection ratios, respectively., Main Conclusions: We show how GPS-tracking data can be used to evaluate IUCN habitat suitability data. Our findings indicate that for the majority of species included in this study, it is appropriate to use IUCN habitat suitability data in macroecological studies. Furthermore, we show that GPS-tracking data can be used to identify and prioritize species and habitat types for re-evaluation of IUCN habitat suitability data., Competing Interests: The authors have no conflict of interest to declare., (© 2022 The Authors. Global Ecology and Biogeography published by John Wiley & Sons Ltd.)

The Blue-fronted Amazon (Amazona aestiva) is a Neotropical parrot with a large distribution in South America, including areas in Brazil, Bolivia, Argentina and Paraguay. A substantial part of the population of this parrot is concentrated in the Pantanal, a large wetland located in the center of South America. There, the clearing of forest and savannas has occurred through the years to accommodate beef-cattle pasture, and crops. Our objective was to understand the direct and indirect effects of time, availability of forest and savannas, and rainfall over the number of nestlings and fledged young of Blue-fronted Amazons inhabiting the southern Pantanal. We surveyed their nests from 1997 to 2018 and counted the number of nestlings produced and the number of fledglings capable to leave the nest in each year. Additionally, we used available data on the area covered by arboreal vegetation, and rainfall data collected at a Meteorological Station located in a central area of the study, as predictors of the numbers of nestlings and fledglings produced every year. Then, we applied structural equation modeling to examine both the direct and indirect effects of time, arboreal habitat availability, and annual cumulative rainfall on the number of nestlings and fledgling per nest. Finally, we estimated the long-term trend of the number of fledglings per nest as a surrogate to the populational trend of Blue-fronted Amazon. We found that none of the variables in our model explained the number of nestlings in nests, but the number of nestlings and the arboreal habitat availability directly and positively affected the number of fledglings. Time indirectly and negatively affected the number of fledglings per nest, whereas rainfall affected it indirectly positively. Additionally, we detected a concerning decrease of about 30% of the fledglings during the 22-years study, which could lead to a change in the species conservational status., Competing Interests: The authors have declared that no competing interests exist. The affiliation of GM with Embrapa Pantanal does not alter our adherence to PLOS ONE policies on sharing data and materials.

The present study aimed to estimate covariance components of milk fatty acids (FA) and to compare the genomic estimated breeding values under general and heat-stress effects. Data consisted of 38,762 test-day records from 6,344 Holstein cows obtained from May 2012 through January 2018 on 4 dairy herds from Brazil. Single-trait repeatability test-day models with random regressions as a function of temperature-humidity index values were used for genetic analyses. The models included contemporary groups, parity order (1-6), and days in milk classes as fixed effects, and general and thermotolerance additive genetic and permanent environmental as random effects. Notably, differences in heritability estimates between environments (general and heat stress) increased (0.03 to 0.06) for unsaturated FA traits, such as unsaturated, monounsaturated, and polyunsaturated, at higher heat-stress levels. In contrast, heritability estimated between environments for saturated FA traits, including saturated FA, palmitic acid (C16:0), and stearic acid (C18:0) did not observe significant differences between environments. In addition, our study revealed negative genetic correlations between general and heat-stress additive genetic effects (antagonistic effect) for the saturated FA, C16:0, C18:0, and C18:1, which ranged from -0.007 to -0.32. Spearman's ranking correlation between genomic estimated breeding values ranged from -0.27 to 0.99. Results indicated a moderate to strong interaction of genotype by the environment for most FA traits comparing a heat-stress environment with thermoneutral conditions. Our findings point out novel opportunities to explore the use of FA milk profile and heat-stress models., (The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)

Mammals can show conspicuous behavioral responses to thermal variation, including changes in movement patterns. We used an integrative approach to understand how environmental temperature can drive the movement behavior of a mammal with low capacity for physiological thermoregulation, the giant anteater (Myrmecophaga tridactyla). We tracked 52 giant anteaters in 7 areas throughout the Brazilian savannah. We estimated the distance moved, area used, use of forest areas, and mean environmental temperature for each monitoring day of each individual. We modeled these data with Mixed Structural Equations - considering the possible interactions between our variables and controlling for sex and body mass. Giant anteaters reduced displacement and increased forest use with decreasing environmental temperature, probably because of their low body heat production. It is possible that they reduce distance moved and area used by reducing the duration of activity. With decreasing temperature, forest habitats become warmer than open ones, besides buffer rain and chilly winds. Reducing displacement and using forests are important strategies to reduce body heat loss and the energetic costs of thermoregulation. However, decreasing movement can limit food access and, consequently, fitness. Therefore, we highlight the importance of forests as thermal shelters. With increasing frequency and intensity of extreme weather events, we showed the need to preserve forest patches to offer suitable conditions for tropical mammals' behavioral thermoregulation. In this context, policies favoring deforestation on Brazilian territory are especially worrisome. Finally, we emphasize the need of integrative approaches to understand the complex interactions between organisms and the environment., (© 2021 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.)

The giant otter, Pteronura brasiliensis, (Zimmermann, 1780), is a semiaquatic carnivore that feeds mainly on fish. Mercury has high toxicity and high potential for bioaccumulation in tissues and biomagnification in organisms through food chains. Thus, as a top predator in the trophic chain, the giant otter has the potential to accumulate mercury by biomagnification. The objective of the present study was to measure the total mercury concentration in giant otter fur samples from an area in the southern Brazilian Pantanal. Fur samples from 19 otters from different social groups were collected from captured animals or were sampled with biopsy darts. Total mercury determination was performed by cold steam spectrophotometry. Mercury concentrations found in the giant otter fur were 7.15 ± 3.41 μg g -1 (2.01-12.06 μg g -1 ) dry weight. The values are above to the upper limit found in fur samples of otter species not exposed to contamination sources, which typically range from 1 to 5 μg g -1 . The concentrations found in the study area indicate that even in the southern Pantanal, which is approximately 475 km from gold mining activity, mercury can be considered a threat for giant otters, as well as for riverside populations. It is also important to consider that other anthropogenic sources of mercury, such as pesticides used in agriculture, could affect this population., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Antithyroglobulin antibodies (TgAb) are present in up to 25% of patients with differentiated thyroid carcinoma on initial postoperative assessment. Detectable concentrations of TgAb even below the manufacturer's cut-off can interfere with serum thyroglobulin (Tg) determination. When Tg is quantified using an immunometric assay (IMA) (hereafter referred to as Tg-IMA), this interference results in underestimated values of Tg. Although promising, more clinical trials evaluating the capacity of liquid chromatography/tandem mass spectrometry and of new assays to detect elevated Tg in patients with TgAb and structural disease are necessary, particularly when Tg is undetectable by a second-generation IMA (Tg-2GIMA). Neck ultrasonography (US) should be performed in patients submitted to total thyroidectomy and with negative Tg-IMA but with detectable TgAb more than 6 months after initial therapy. In patients treated with 131I, comparison of TgAb concentrations obtained before this treatment is useful to estimate the risk of disease and to guide the investigation. If initial assessment does not reveal any persistent tumor, the repetition of US is recommended while TgAb persist. Significant elevation of TgAb requires extended investigation. On the other hand, patients with negative Tg-IMA and US without abnormalities who exhibit a reduction > 50% in TgAb generally do not require investigation. Although TgAb can interfere with Tg, the management and follow-up of patients submitted to total thyroidectomy with borderline TgAb can probably be the same as those recommended for patients without TgAb if Tg-2GIMA and US indicate an excellent response to therapy. Currently, the presence/absence or the trend of TgAb levels cannot be considered in the follow-up of patients submitted to lobectomy.

Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal-central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus, Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. (n = 37,782), Sus scrofa (n = 6,730), and Canis familiaris (n = 10,084); 17 species were represented by only one record (e.g., Syncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans). Primates have the highest number of species in the data set (n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata, and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation-related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data., (© 2020 The Authors. Ecology © 2020 The Ecological Society of America.)

Accurately quantifying species' area requirements is a prerequisite for effective area-based conservation. This typically involves collecting tracking data on species of interest and then conducting home-range analyses. Problematically, autocorrelation in tracking data can result in space needs being severely underestimated. Based on the previous work, we hypothesized the magnitude of underestimation varies with body mass, a relationship that could have serious conservation implications. To evaluate this hypothesis for terrestrial mammals, we estimated home-range areas with global positioning system (GPS) locations from 757 individuals across 61 globally distributed mammalian species with body masses ranging from 0.4 to 4000 kg. We then applied block cross-validation to quantify bias in empirical home-range estimates. Area requirements of mammals <10 kg were underestimated by a mean approximately15%, and species weighing approximately100 kg were underestimated by approximately50% on average. Thus, we found area estimation was subject to autocorrelation-induced bias that was worse for large species. Combined with the fact that extinction risk increases as body mass increases, the allometric scaling of bias we observed suggests the most threatened species are also likely to be those with the least accurate home-range estimates. As a correction, we tested whether data thinning or autocorrelation-informed home-range estimation minimized the scaling effect of autocorrelation on area estimates. Data thinning required an approximately93% data loss to achieve statistical independence with 95% confidence and was, therefore, not a viable solution. In contrast, autocorrelation-informed home-range estimation resulted in consistently accurate estimates irrespective of mass. When relating body mass to home range size, we detected that correcting for autocorrelation resulted in a scaling exponent significantly >1, meaning the scaling of the relationship changed substantially at the upper end of the mass spectrum., (© 2020 The Authors. Conservation Biology published by Wiley Periodicals LLC on behalf of Society for Conservation Biology.)

Spatial ecology data are essential for conservation purposes, especially when extinction risk is influenced by anthropogenic actions. Space use can reveal how individuals use the habitat, how they organize in space, and which components are key resources for the species.We evaluated the space use and multiscale habitat selection of giant anteaters ( Myrmecophaga tridactyla ), a vulnerable Neotropical mammal, in a Cerrado site within a human-modified landscape in southeastern Brazil.We used GPS transmitters to track eight anteaters in the wild. With the resulting dataset, we estimated home range and core-area sizes and then used two overlap indexes. We assessed habitat selection by compositional analysis and analyzed events of spatio-temporal proximity.The average Brownian bridge kernel estimate of home range size was 3.41 km 2 (0.92-7.9). Regarding home range establishment, five individuals showed resident behavior. Males ( n  = 4) had larger home ranges and were more active than females ( n  = 4). Despite the spatial overlap of home range (above 40% in four dyads), maximum temporal space sharing was 18%. Giant anteaters were found in proximity. Habitat selection favored savanna, and exotic timber plantation was always avoided. Roads and built-up areas were selected secondarily at the landscape level.The selection of anthropogenic sites denotes behavioral plasticity regarding modified habitats. However, the high selectivity for savanna, at all levels, demonstrates a high dependence on natural habitats, which provide the necessary resources for the species. The recurrent proximity of male-to-female anteaters may indicate reproductive behavior, which is essential for maintaining this isolated population., Competing Interests: None declared., (© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)

The weaning process may cause intense stress for dairy calves, even when low volumes of liquid diet are fed. Management tools that increase the intake of solid feeds, such as gradual weaning, can provide better physiological and metabolic conditions through better ruminal development, leading to better adaptation to ruminant metabolism and aiding in stress mitigation. The objective of this study was to evaluate the effects of 2 weaning protocols and 2 levels of concentrate intake on the performance and physiological and behavioral variables related to stress in dairy calves. Thirty-six newborn male Holstein calves were used in a randomized block design with a 2 × 2 factorial arrangement: 2 weaning strategies, abrupt or gradual, and 2 levels of concentrate intake at 5 wk of age, high (>350 g/d) or low (≤350 g/d). Calves were equally managed until they were 5 wk of age and then grouped according to concentrate intake. Statistical analyzes were performed using the MIXED procedure of SAS software (SAS Institute Inc., Cary, NC), and no significant interaction was observed between studied factors (weaning method and starter intake level); therefore, we considered each factor separately and their interactions with age. The highest dry matter intake and concentration of β-hydroxybutyrate were recorded for animals with a high level of starter intake independent of the weaning method. Structural growth (cm/wk) and average daily gain were superior for calves with high starter intake, but weaning method had no effect. The gradual weaning protocol increased the time eating starter, regardless of the level of concentrate intake. Even animals with low concentrate intake that were weaned abruptly showed levels of cortisol and acid-soluble glycoprotein within normal physiological levels. Apparently, other factors besides the milk supply affect the starter intake level of calves in a conventional feeding program. The adoption of gradual weaning is not effective in improving performance when a calf has low intake 3 wk before weaning is complete, but it reduced vocalization on d 2 postweaning., (Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Background: Most patients with papillary thyroid cancer (PTC) and lymph node metastases (LNM) undergoing reoperation do not show apparent disease after this procedure, but serum thyroglobulin (Tg) continues to be elevated in part of them. This study evaluated adjuvant therapy with 131 I in these patients., Methods: Patients with PTC and LNM diagnosed after initial therapy were selected. Patients undergoing reoperation and those without apparent disease after this procedure, but with nonstimulated Tg ≥1 ng/ml 6 months after reoperation, were included. The first 25 patients were submitted to therapy with 131 I (groups A) and the subsequent 30 patients did not receive this therapy (group B)., Results: Groups A and B were similar. During further follow-up, 21 patients developed structural disease and 34 continued without detectable disease (eight achieved complete remission). The outcomes were similar in groups A and B. Patients with Tg reduction >50% after reoperation tended to have a lower risk of recurrence (22.7 versus 48.5%), notably distant metastases (0 versus 15.1%), and were more likely to achieve complete remission (28 versus 3%). Patients with LNM FDG-positive had a higher risk of recurrence (54.5 versus 11.7%) and were less likely to achieve complete remission (3 versus 29.4%) after reoperation., Conclusion: Our results suggest that therapy with 131 I apparently does not prevent recurrences among patients who continue to have elevated Tg after neck reoperation. Further studies involving these patients are necessary, especially those who are at high risk of recurrence.

Distinct species of Trypanosoma have been documented sharing the same hosts in different environments in intricate transmission networks. Knowing this, this study investigated the role of different hosts in the transmission cycles of Trypanosoma species in the Pantanal biome. The mammals were sampled from November 2015 to October 2016. We sampled a total of 272 wild mammals from 27 species belonging to six orders and 15 families, and three species of triatomines (n = 7). We found high parasitemias by Hemoculture test for Trypanosoma cruzi (TcI), Trypanosoma rangeli, Trypanosoma cruzi marinkellei and Trypanosoma dionisii, and high parasitemias by Microhematocrit Centrifuge Technique for Trypanosoma evansi. The carnivore Nasua nasua is a key host in the transmission cycles since it displayed high parasitemias for T. cruzi, T. evansi and T. rangeli. This is the first report of high parasitemias in Tamandua tetradactyla and cryptic infection in Dasypus novemcinctus by T. cruzi; cryptic infection by T. evansi in Eira barbara, Euphractus sexcinctus and Dasyprocta azarae. The collection of Panstrongylus geniculatus increased the geographic distribution of this vector species in the South America. Our results indicate that Trypanosoma species circulate in a complex reservoir system including different host species with different infective competences., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Xenarthrans-anteaters, sloths, and armadillos-have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths. Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the southern United States, Mexico, and Caribbean countries at the northern portion of the Neotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n = 5,941), and Cyclopes sp. have the fewest (n = 240). The armadillo species with the most data is Dasypus novemcinctus (n = 11,588), and the fewest data are recorded for Calyptophractus retusus (n = 33). With regard to sloth species, Bradypus variegatus has the most records (n = 962), and Bradypus pygmaeus has the fewest (n = 12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other data sets of Neotropical Series that will become available very soon (i.e., Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans data set. Please cite this data paper when using its data in publications. We also request that researchers and teachers inform us of how they are using these data., (© 2019 The Authors. Ecology © 2019 The Ecological Society of America.)