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Precise, reliable, and speedy contamination detection and disinfection is an ongoing challenge for the food-service industry. Contamination in food-related services can cause foodborne illness, endangering customers and jeopardizing provider reputations. Fluorescence imaging has been shown to be capable of identifying organic residues and biofilms that can host pathogens. We use new fluorescence imaging technology, applying Xception and DeepLabv3+ deep learning algorithms to identify and segment contaminated areas in images of equipment and surfaces. Deep learning models demonstrated a 98.78% accuracy for differentiation between clean and contaminated frames on various surfaces and resulted in an intersection over union (IoU) score of 95.13% for the segmentation of contamination. The portable imaging system’s intrinsic disinfection capability was evaluated on S. enterica, E. coli, and L. monocytogenes, resulting in up to 8-log reductions in under 5 s. Results showed that fluorescence imaging with deep learning algorithms could help assure safety and cleanliness in the food-service industry.

The appropriate methodological approach for intestinal preparation enables researchers to create representative histological and immunostaining images which validate their biochemical data. The Swiss-roll technique was first introduced by Reilly and Kirsner in 1965. Later, Moolenbeek and Ruitenberg described a detailed procedure to longitudinally study the rodent intestine in 1981 [1]. In this publication, our slightly different approach for co-embedding four different Swiss-rolls in a gelatin block provides a full-length overview of cross-sectional colons on a single slide. This protocol allows for longitudinal histologic examination of multiple tissue samples on a single slide simultaneously. In this method, antigenicity is retained for immunohistology. In addition, the accessibility of samples during the prolonged hardening time required of the gelatin matrix allows the tissue samples to be adjusted/re-adjusted to provide the desired orientation and spatial arrangement for ideal cross sections with similar planes of section and optimum space utilization for slide mounting. Although not the focus of this protocol, the room temperature stability of the gelatin matrix and the ability to contain numerous tissue samples in a block allows the flexibility of performing thicker sections for free-floating tissue staining and ease of mounting a single gelatin sheet rather than individual tissue sections. This is a convenient approach for allowing precise preparation of multi-tissue blocks and simultaneous sectioning, staining, and slide mounting of tissue for subsequent comparisons. • A single gelatin block is prepared by simultaneously embedding at least four different intestinal Swiss-rolls. • The tissue orientation can be adjusted for each sample as desired which facilitates the comparison of different colon samples on a single gelatin section. • The gelatin sections containing tissue samples are stable at least overnight at room temperature for staining. Method name: Gelatin embedded Swiss-rolls, Keywords: Longitudinal histologic exam, Colon, Gelatin embedded sections

Objectives: Traumatic dental injuries (TDIs) commonly occur in sport clubs. The knowledge and performance of fitness trainers play an important role in management of such injuries. This study sought to assess the effect of an educational pamphlet on knowledge level and performance of fitness trainers about TDIs in Tehran in 2018. Materials and Methods: In this interventional study, a pamphlet was designed to enhance the knowledge level of fitness trainers. Ninety-five fitness trainers were randomly divided into two groups of intervention and control (n=49 in the control group and n=46 in the interventional group), and were requested to fill out a valid and reliable researcher-designed questionnaire about TDIs before and 1 month after pamphlet distribution. The questionnaire consisted of three domains of demographic information, knowledge questions, and performance questions. The results were analyzed using SPSS 25 via the Chi-square test and repeated measures ANOVA considering the intervention as the between-subject factor. Results: The knowledge score of fitness trainers about TDIs was not adequately high in the intervention or the control group before the intervention. After the intervention, the performance of participants improved in both groups. This increase was significantly greater in the intervention group (P=0.035). There was no significant difference between the two groups in the knowledge domain (P=0.185). Conclusion: Educational pamphlets can effectively enhance the knowledge level of fitness trainers about TDIs. However, the magnitude of this effect was not significant in our study. Future studies are recommended to compare the efficacy of educational pamphlets with other educational tools.

Aging is a major risk factor for Alzheimer’s disease (AD). Insulin-like growth factor-1 receptor (IGF-1R) regulates general aging and lifespan. However, the contribution of IGF-1 to age-related AD pathology and progression is highly controversial. Based on our previous work, AβPP/PS1 double transgenic mice, which express human mutant amyloid precursor protein (APP) and presenilin-1 (PS-1), demonstrated a decrease in brain IGF-1 levels when they were crossed with IGF-1 deficient Ames dwarf mice (df/df). Subsequently, a reduction in gliosis, amyloid-β (Aβ) plaque deposition, and Aβ1–40/42 concentrations were observed in this mouse model. This supported the hypothesis that IGF-1 may contribute to the progression of the disease. To assess the role of IGF-1 in AD, 9–10-month-old male littermate control wild type and AβPP/PS1 mice were randomly divided into two treatment groups including control vehicle (DMSO) and picropodophyllin (PPP), a selective, competitive, and reversible IGF-1R inhibitor. The brain penetrant inhibitor was given ip. at 1 mg/kg/day. Mice were sacrificed after 7 days of daily injection and the brains, spleens, and livers were collected to quantify histologic and biochemical changes. The PPP-treated AβPP/PS1 mice demonstrated attenuated insoluble Aβ1–40/42. Additionally, an attenuation in microgliosis and protein p-tyrosine levels was observed due to drug treatment in the hippocampus. Our data suggest IGF-1R signaling is associated with disease progression in this mouse model. More importantly, modulation of the brain IGF-1R signaling pathway, even at mid-life, was enough to attenuate aspects of the disease phenotype. This suggests that small molecule therapy targeting the IGF-1R pathway may be viable for late-stage disease treatment.

Introduction: Revascularization is the newest treatment proposed for an immature permanent tooth with necrotic pulp. Revascularization is defined as a treatment process with a biological base which is aimed at replacing the lost structures and the continued development of roots. Case presentation: Patient was an eight-year-old child with the history of trauma to the maxillary anterior region. After clinical and radiographic evaluations, tooth #9 was diagnosed with the pulp necrosis, and revascularization was carried out according to Banch and Trope principles. Conclusion: The clinical and radiographic evaluation after treatment revealed no signs and symptoms and root development was in progress which was an indication of successful treatment. It seems that, in the case of proper selection of the patient, this method can be an appropriate replacement for apexification in immature teeth with pulp necrosis.

Background: Immediate implant placement has advantages such as requiring fewer surgical procedures and decreased treatment time; however, unpredictable soft- and hard-tissue outcome is a problem. This study aimed to compare the soft-tissue esthetic outcome of single implants placed in fresh extraction sockets versus those placed in healed sockets. Materials and Methods: This cross-sectional, retrospective study was performed on 42 patients who received single implants. Twenty-two patients with a mean age of 40.14 years received immediate implants while 18 patients with a mean age of 43.40 years were subjected to conventional (delayed) implant placement. The mean follow-up time was 14.42 ± 8.37 months and 18.25 ± 7.10 months in the immediate and conventional groups, respectively. Outcome assessments included clinical and radiographic examinations. The esthetic outcome was objectively rated using the pink esthetic score (PES). Results: All implants fulfilled the success criteria. The mean PES was 8.54 ± 1.26 and 8.10 ± 1.65 in the immediate and conventional groups, respectively. This difference was not statistically significant (P = 0.329). The two PES parameters, namely, the facial mucosa curvature and facial mucosa level had the highest percentage of complete score. Conclusions: Immediate and conventional single implant treatments yielded comparable esthetic outcomes.

Objectives: This study aimed to assess the microleakage of Pedo Jacket crowns compared to stainless steel crowns (SSCs) cemented with different cements. Materials and Methods: In this in vitro experimental study, 80 primary molars were randomly divided into four groups of 20. Groups 1 and 2 were subjected to standard tooth preparation for SSC. Crowns in group 1 were cemented with glass ionomer (GI) and crowns in group 2 were cemented with resin modified glass ionomer (RMGI) cement. In groups 3 and 4, minimal tooth preparation was performed for Pedo Jacket crowns, and the crowns were cemented with RMGI and Panavia resin cement, respectively. Microleakage was measured in mesial and distal surfaces in micrometers, and the mean value for each tooth was calculated. One-way ANOVA was applied to compare the microleakage of the four groups. Results: Group 3 (Pedo Jacket cemented with RMGI) showed the highest microleakage (1523.83±250.32 µm) with significant differences with the remaining three groups (P