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The defect investigation of sluice floor is an important measure to ensure the safe operation of the sluice. due to the traditional drilling methods will cause damage to the sluice floor, and the appearance inspection and compressive strength test can not evaluate the internal defects of reinforced concrete in the sluice floor. In this paper, we apply the ground penetrating radar(GPR) to the concrete quality inspection of a sluice gate in a seawall. And we describe the layout, detection principle and data processing flow of GPR. Then we simulate the propagation characteristics of radar electromagnetic wave in the presence of twodimensional electrical anomaly bodies by finite difference method. Through analyzing the amplitude, frequency and event characteristics of reflection wave shown in the gray map of GPR profiles, we determine the dividing line between concrete and muddy silty clay, finding out the density of steel in concrete, framing the location of construction joints and suspected water-filled gaps.