2,009 results on '"Ming, YAO"'
Search Results
2. Upper Gastrointestinal Involvement in Crohn’s Disease as an Independent Predictor of New-Onset Strictures: Insights from an Extensive Retrospective Cohort Analysis
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Hsu, Wei-Chun, Chen, Chien-Ming, Kuo, Chia-Jung, Tsou, Yung-Kuan, Yeh, Pai-Jui, Lai, Ming‑Wei, Wu, Ren-Chin, Chiu, Cheng-Tang, Su, Ming‑Yao, Pan, Yu-Bin, and Le, Puo-Hsien
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- 2024
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3. Temporal Trends of Inflammatory Bowel Diseases in Taiwan from 2016 to 2020: A Population-Based Study
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Kuo, Chia-Jung, Lin, Cheng-Yu, Le, Puo-Hsien, Kuo, Yao-Wei, Hsu, Chen-Ming, Lai, Ming-Wei, Lin, Wey-Ran, Chang, Ming-Ling, Su, Ming-Yao, Chiu, Cheng-Tang, and Chang, Chee-Jen
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- 2024
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4. Risk factor differences in five-year progression of Intracranial artery stenosis and cerebral small vessel disease in general population
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Zi-Ang Pan, Ding-Ding Zhang, Zi-Yue Liu, Mei-Jun Shu, Fei-Fei Zhai, Ming Yao, Li-Xin Zhou, Jun Ni, Zheng-Yu Jin, Shu-Yang Zhang, Li-Ying Cui, Fei Han, and Yi-Cheng Zhu
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Intracranial artery stenosis ,Cerebral small vessel disease ,Cohort study ,Longitudinal study ,Risk factors ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Intracranial artery stenosis (ICAS) and cerebral small vessel disease (CSVD) are associated with a heavy socioeconomic burden; however, their longitudinal changes remain controversial. Methods We conducted a longitudinal analysis on 756 participants of Shunyi Cohort who underwent both baseline and follow-up brain magnetic resonance imaging (MRI) and MR angiography in order to investigate the risk factors for ICAS and CSVD progression in community population. Incident ICAS was defined as new stenosis occurring in at least one artery or increased severity of the original artery stenosis. CSVD markers included lacunes, cerebral microbleeds (CMB), and white matter hyperintensities (WMH). Results After 5.58 ± 0.49 years of follow-up, 8.5% of the 756 participants (53.7 ± 8.0 years old, 65.1% women) had incident ICAS. Body mass index (BMI) (OR = 1.09, 95% CI = 1.01–1.17, p = 0.035) and diabetes mellitus (OR = 2.67, 95% CI = 1.44–4.93, p = 0.002) were independent risk factors for incident ICAS. Hypertension was an independent risk factor for incident lacunes (OR = 2.12, 95% CI = 1.20–3.77, p = 0.010) and CMB (OR = 2.32, 95% CI = 1.22–4.41, p = 0.011), while WMH progression was primarily affected by BMI (β = 0.108, SE = 0.006, p = 0.002). A higher LDL cholesterol level was found to independently protect against WMH progression (β = −0.076, SE = 0.027, p = 0.019). Conclusions Modifiable risk factor profiles exhibit different in patients with ICAS and CSVD progression. Controlling BMI and diabetes mellitus may help to prevent incident ICAS, and antihypertensive therapy may conduce to mitigate lacunes and CMB progression. LDL cholesterol may play an inverse role in large arteries and small vessels.
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- 2024
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5. HMGB1 inhibition blocks ferroptosis and oxidative stress to ameliorate sepsis‐induced acute lung injury by activating the Nrf2 pathway
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Ya‐Jie Jia, Sha Xiong, Ming Yao, Yu Wei, and Yan He
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acute lung injury ,ferroptosis ,HMGB1 ,Nrf2 ,sepsis ,Medicine (General) ,R5-920 - Abstract
Abstract The proinflammatory properties of high‐mobility group box protein 1 (HMGB1) in sepsis have been extensively studied. This study aimed to investigate the impact of HMGB1 on ferroptosis and its molecular mechanism in sepsis‐induced acute lung injury (ALI). A septic mouse model was established using the cecal ligation and puncture method. Blocking HMGB1 resulted in improved survival rates, reduced lung injury, decreased levels of ferroptosis markers (reactive oxygen species, malondialdehyde, and Fe2+), and enhanced antioxidant enzyme activities (superoxide dismutase and catalase) in septic mice. In addition, knockdown of HMGB1 reduced cellular permeability, ferroptosis markers, and raised antioxidant enzyme levels in lipopolysaccharide (LPS)‐stimulated MLE‐12 cells. Silencing of HMGB1 led to elevations in the expressions of ferroptosis core‐regulators in LPS‐treated MLE‐12 cells, such as solute carrier family 7 member 11 (SLC7A11), solute carrier family 3 member A2 (SLC3A2), and glutathione peroxidase 4. Furthermore, blocking HMGB1 did not alter ferroptosis, oxidative stress‐related changes, and permeability in LPS‐treated MLE‐12 cells that were pretreated with ferrostatin‐1 (a ferroptosis inhibitor). HMGB1 inhibition also led to elevated expressions of nuclear factor erythroid 2‐related factor 2 (Nrf2) and its downstream targets, heme oxygenase‐1 (HO‐1) and NAD(P)H: quinone oxidoreductase 1 (NQO1) in LPS‐treated MLE‐12 cells and lung tissues from septic mice. The Nrf2‐specific inhibitor ML385 reversed the effects of HMGB1 silencing on ferroptosis and cell permeability in LPS‐treated MLE‐12 cells. Our findings indicated that the inhibition of HMGB1 restrains ferroptosis and oxidative stress, thereby alleviating sepsis‐induced ALI through the activation of Nrf2 signaling.
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- 2024
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6. Bacteria extracellular vesicle as nanopharmaceuticals for versatile biomedical potential
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Ming Yao Ho, Songhan Liu, and Bengang Xing
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Bacterial extracellular vesicle ,Nanomedicine ,Drug delivery ,Vaccination ,Nanotechnology ,Immunotherapy ,Technology ,Chemical technology ,TP1-1185 ,Biotechnology ,TP248.13-248.65 ,Science ,Physics ,QC1-999 - Abstract
Abstract Bacteria extracellular vesicles (BEVs), characterized as the lipid bilayer membrane-surrounded nanoparticles filled with molecular cargo from parent cells, play fundamental roles in the bacteria growth and pathogenesis, as well as facilitating essential interaction between bacteria and host systems. Notably, benefiting from their unique biological functions, BEVs hold great promise as novel nanopharmaceuticals for diverse biomedical potential, attracting significant interest from both industry and academia. Typically, BEVs are evaluated as promising drug delivery platforms, on account of their intrinsic cell-targeting capability, ease of versatile cargo engineering, and capability to penetrate physiological barriers. Moreover, attributing to considerable intrinsic immunogenicity, BEVs are able to interact with the host immune system to boost immunotherapy as the novel nanovaccine against a wide range of diseases. Towards these significant directions, in this review, we elucidate the nature of BEVs and their role in activating host immune response for a better understanding of BEV-based nanopharmaceuticals’ development. Additionally, we also systematically summarize recent advances in BEVs for achieving the target delivery of genetic material, therapeutic agents, and functional materials. Furthermore, vaccination strategies using BEVs are carefully covered, illustrating their flexible therapeutic potential in combating bacterial infections, viral infections, and cancer. Finally, the current hurdles and further outlook of these BEV-based nanopharmaceuticals will also be provided. Graphical Abstract
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- 2024
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7. The genus Nisotra Baly, 1864 (Coleoptera, Chrysomeliae, Galerucinae, Alticini) in Taiwan, with redescriptions of four Asian species and notes on the immature stages of N. gemella (Erichson, 1834)
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Chi-Feng Lee, Ming-Yao Chiang, Michael F. Geiser, and Kuo-Hung Chuang
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Zoology ,QL1-991 - Abstract
Nisotra chrysomeloides Jacoby, 1885, N. dohertyi (Maulik, 1926), N. gemella (Erichson, 1834), and Nisotra nigripes Jacoby, 1894 are redescribed with illustrations of aedeagi, antennae, gonocoxae, abdominal ventrite VIII, and spermathecae. Nisotra nigripes is recorded for the first time from Taiwan. The immature stages and life history of N. gemella were studied in the laboratory using a novel rearing design. Four synonyms previously proposed are confirmed: Sphaeroderma javana de Motschulsky, 1866, S. orbiculata de Motschulsky, 1866, Nisotra bowringi Baly, 1876, and Podagrica hibisci Bryant, 1941 with N. gemella (Erichson, 1834). Lectotypes are designated for Haltica gemella Erichson, 1834, N. chrysomeloides Jacoby, 1885, N. bowringi Baly, 1876, and Podagrica hibisci Bryant, 1941.
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- 2024
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8. Bacteria extracellular vesicle as nanopharmaceuticals for versatile biomedical potential
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Ho, Ming Yao, Liu, Songhan, and Xing, Bengang
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- 2024
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9. Microbiome signatures associated with clinical stages of gastric Cancer: whole metagenome shotgun sequencing study
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Jeong, Sohyun, Liao, Yi-Tyng, Tsai, Min-Hsuan, Wang, Yao-Kuang, Wu, I-Chen, Liu, Chung-Jung, Wu, Ming-Shun, Chan, Tze-Sian, Chen, Ming-Yao, Hu, Ping-Jen, Kao, Wei-Yu, Liu, Hsiang-Chin, Tsai, Ming-Ju, Liu, Cheng-Yuan, Chang, Chun-Chao, Wu, Deng-Chyang, and Hsu, Yi-Hsiang
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- 2024
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10. Applying narrative medicine to prepare empathetic healthcare providers in undergraduate pharmacy education in Singapore: a mixed methods study
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Han, Zhe, Barton, Keith C., Ho, Li-Ching, Yap, Kai Zhen, Tan, Doreen Su-Yin, Lee, Shuh Shing, Neo, Constance Xue Rui, Tan, Amanda Han Lin, Boey, Brian Ming Yao, Soon, Charis Jia Yan, and Gallagher, Paul J.
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- 2024
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11. Mirrorless MEMS imaging: a nonlinear vibrational approach utilizing aerosol-jetted PZT-actuated fiber MEMS scanner for microscale illumination
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Wang, Wei-Chih, Li, Ming-Yao, Peng, Kuan-Chang, Hsu, Yi-Feng, Estroff, Benjamin, Yen, Pao-Yun, Schipf, David, and Wu, Wen-Jong
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- 2024
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12. The genetic risk factors for cerebral venous thrombosis: a case-control study in a Chinese national comprehensive hospital
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Shaoying Wang, Ming Yao, Xinzhuang Yang, Yicheng Zhu, and Bin Peng
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Cerebral venous thrombosis ,Whole exome sequencing ,Risk factor ,Hereditary thrombophilia ,MAP kinase signaling pathway ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Abstract Background About 13–25% of cerebral venous thrombosis (CVT) cases lack clear etiology, which may be associated with underlying genetic factors. This study aims to investigate genetic factors in CVT patients using whole exome sequencing (WES). Methods Thirty-eight CVT patients hospitalized underwent WES. 977 subjects with WES data from a community cohort study --the Shunyi cohort were as the control group. Using bioinformatics analysis, differential genes with rare damaging variants between two groups were filtered (P A, CBS c.949 A > G; F2 c.1787G > A; SERPINC1 c.409-11G > T. Comparing the WES data of two groups, a total of 179 different genes with rare damaging variants were screened (P
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- 2024
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13. Distinct characteristics of the gut virome in patients with osteoarthritis and gouty arthritis
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Chang-Ming Chen, Qiu-Long Yan, Ruo-Chun Guo, Fang Tang, Min-Hui Wang, Han-Zhi Yi, Chun-Xia Huang, Can Liu, Qiu-Yi Wang, Wei-Ya Lan, Zong Jiang, Yu-Zheng Yang, Guang-Yang Wang, Ai-Qin Zhang, Jie Ma, Yan Zhang, Wei You, Hayan Ullah, Yue Zhang, Sheng-Hui Li, Xue-Ming Yao, Wen Sun, and Wu-Kai Ma
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Osteoarthritis ,Gouty arthritis ,Gut virome ,Virus-like particle-based metagenome ,Viral diversity ,Viral dysbiosis ,Medicine - Abstract
Abstract Background/purpose(s) The gut microbiota and its metabolites play crucial roles in pathogenesis of arthritis, highlighting gut microbiota as a promising avenue for modulating autoimmunity. However, the characterization of the gut virome in arthritis patients, including osteoarthritis (OA) and gouty arthritis (GA), requires further investigation. Methods We employed virus-like particle (VLP)-based metagenomic sequencing to analyze gut viral community in 20 OA patients, 26 GA patients, and 31 healthy controls, encompassing a total of 77 fecal samples. Results Our analysis generated 6819 vOTUs, with a considerable proportion of viral genomes differing from existing catalogs. The gut virome in OA and GA patients differed significantly from healthy controls, showing variations in diversity and viral family abundances. We identified 157 OA-associated and 94 GA-associated vOTUs, achieving high accuracy in patient-control discrimination with random forest models. OA-associated viruses were predicted to infect pro-inflammatory bacteria or bacteria associated with immunoglobulin A production, while GA-associated viruses were linked to Bacteroidaceae or Lachnospiraceae phages. Furthermore, several viral functional orthologs displayed significant differences in frequency between OA-enriched and GA-enriched vOTUs, suggesting potential functional roles of these viruses. Additionally, we trained classification models based on gut viral signatures to effectively discriminate OA or GA patients from healthy controls, yielding AUC values up to 0.97, indicating the clinical utility of the gut virome in diagnosing OA or GA. Conclusion Our study highlights distinctive alterations in viral diversity and taxonomy within gut virome of OA and GA patients, offering insights into arthritis etiology and potential treatment and prevention strategies. Graphical Abstract
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- 2024
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14. Ten-year epidemiology and risk factors of cytomegalovirus infection in hematopoietic stem cell transplantation patients in Taiwan
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Yi-Che Huang, Fei-Yuan Hsiao, Shang-Ting Guan, Ming Yao, Chia-Jen Liu, Tzu-Ting Chen, Tung-Liang Lin, Yi-Chang Liu, Tsai-Yun Chen, Ying-Chung Hong, Ming-Chun Ma, Tran-Der Tan, Chuan-Cheng Wang, Yi-Ying Wu, Po-Wei Liao, Yi-Feng Wu, Yi-Yang Chen, Yuan-Bin Yu, Yao-Yu Hsieh, Ming-Yang Lee, Jia-Hau Liu, Shu-Wen Lin, and Bor-Sheng Ko
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CMV disease ,CMV infection ,HSCT ,Microbiology ,QR1-502 - Abstract
Background: Cytomegalovirus (CMV) can cause infection and critical diseases in hematopoietic stem cell transplantation (HSCT) recipients. This study aimed to explore the cumulative incidence and risk factors for CMV infection and disease among HSCT recipients in Taiwan. Methods: This retrospective cohort study using the Taiwan Blood and Marrow Transplantation Registry (TBMTR) included HSCT recipients between 2009 and 2018 in Taiwan. The primary outcome was cumulative incidence of CMV infection or disease at day 100 after HSCT. Secondary outcomes included day 180 cumulative incidence of CMV infection or disease, infection sites, risk factors for CMV infection or disease, survival analysis, and overall survival after CMV infection and disease. Results: There were 4394 HSCT recipients included in the study (2044 auto-HSCT and 2350 allo-HSCT). The cumulative incidence of CMV infection and disease was significantly higher in allo-HSCT than in auto-HSCT patients at day 100 (53.7% vs. 6.0%, P
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- 2024
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15. Clinical manifestations and risk factors for COVID-19 and its severity in patients with hematological malignancies
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Tzong-Yow Wu, Wan-Ting Tsai, Kai-Hsiang Chen, Szu-Ting Huang, Chun-Fu Huang, Po-Hsien Kuo, Ming-Jui Tsai, Wang-Da Liu, Kuan-Yin Lin, Yu-Shan Huang, Aristine Cheng, Pao-Yu Chen, Hsin-Yun Sun, Huai-Hsuan Huang, Tai-Chung Huang, Shang-Ju Wu, Ming Yao, Jann-Tay Wang, Wang-Huei Sheng, Chien-Ching Hung, Yee-Chun Chen, and Shan-Chwen Chang
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SARS-CoV-2 ,Respiratory failure ,Lymphoma ,Stem cell transplant ,Vaccination ,Microbiology ,QR1-502 - Abstract
Background: Patients with hematological malignancies (HM) were at a high risk of developing severe disease from coronavirus disease 2019 (COVID-19). We aimed to assess the clinical outcome of COVID-19 in hospitalized patients with HM. Methods: Adult patients with HM who were hospitalized with a laboratory-confirmed COVID-19 between May, 2021 and November, 2022 were retrospectively identified. Primary outcome was respiratory failure requiring mechanical ventilation or mortality within 60 days after hospitalization. We also analyzed associated factors for de-isolation (defined as defervescence with a consecutive serial cycle threshold value > 30) within 28 days. Results: Of 152 eligible patients, 22 (14.5%) developed respiratory failure or mortality in 60 days. Factors associated with developing respiratory failure that required mechanical ventilation or mortality included receipt of allogeneic hematopoietic stem-cell transplantation (allo-HSCT) (adjusted hazards ratio [aHR], 5.10; 95% confidence interval [CI], 1.64–15.85), type 2 diabetes mellitus (aHR, 2.47; 95% CI, 1.04–5.90), lymphopenia at admission (aHR, 6.85; 95% CI, 2.45–19.15), and receiving
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- 2024
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16. The risk profiles of pregnancy-related cerebral venous thrombosis: a retrospective study in a comprehensive hospital
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Shaoying Wang, Ming Yao, Nan Hu, Juntao Liu, and Bin Peng
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Cerebral venous thrombosis ,Pregnancy ,Risk factor ,Thrombophilia ,Antiphospholipid syndrome ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Objectives To investigate the risk factors and underlying causes of pregnancy-related cerebral venous thrombosis (PCVT). Methods A retrospective cohort of 16 patients diagnosed with CVT during pregnancy and postpartum (within six weeks after delivery) in a comprehensive hospital in China between 2009 and 2022 were carefully reviewed, focusing on demographic, clinical, and etiological characteristics, especially underlying causes. We matched 16 PCVT patients with 64 pregnant and puerperal women without PCVT to explore risk factors and clinical susceptibility to PCVT. Results PCVT occurred commonly during the first trimester (43.75%) and the puerperium (37.5%). The frequency of anemia, thrombocytosis and thrombocytopenia during pregnancy, dehydration, and pre-pregnancy anemia was significantly higher in women with PCVT than in those without PCVT (P A (p. Met406Ile), heterozygous PROS1 c.301C > T (p. Arg101Cys), composite heterozygous mutation in the F8 gene (c.144-1259C > T; c.6724G > A (p. Val2242Met)) and homozygous MTHFR c.677C > T (p. Ala222Val). Conclusions The occurrence of anemia, thrombocytopenia and thrombocytosis during pregnancy, dehydration and pre-pregnancy anemia suggested a greater susceptibility to PCVT. For confirmed PCVT patients, autoimmune diseases, hereditary thrombophilia, and hematological disorders were common causes. Screening for potential etiologies should be paid more attention, as it has implications for treatment and long-term management.
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- 2024
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17. Dysregulated dendritic cells in sepsis: functional impairment and regulated cell death
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Li-yu Zheng, Yu Duan, Peng-yi He, Meng-yao Wu, Shu-ting Wei, Xiao-hui Du, Ren-qi Yao, and Yong-ming Yao
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Sepsis ,Dendritic cells ,Functional impairment ,Regulated cell death ,Immunomodulation ,Cytology ,QH573-671 - Abstract
Abstract Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Studies have indicated that immune dysfunction plays a central role in the pathogenesis of sepsis. Dendritic cells (DCs) play a crucial role in the emergence of immune dysfunction in sepsis. The major manifestations of DCs in the septic state are abnormal functions and depletion in numbers, which are linked to higher mortality and vulnerability to secondary infections in sepsis. Apoptosis is the most widely studied pathway of number reduction in DCs. In the past few years, there has been a surge in studies focusing on regulated cell death (RCD). This emerging field encompasses various forms of cell death, such as necroptosis, pyroptosis, ferroptosis, and autophagy-dependent cell death (ADCD). Regulation of DC’s RCD can serve as a possible therapeutic focus for the treatment of sepsis. Throughout time, numerous tactics have been devised and effectively implemented to improve abnormal immune response during sepsis progression, including modifying the functions of DCs and inhibiting DC cell death. In this review, we provide an overview of the functional impairment and RCD of DCs in septic states. Also, we highlight recent advances in targeting DCs to regulate host immune response following septic challenge. Graphical Abstract
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- 2024
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18. Microbiome signatures associated with clinical stages of gastric Cancer: whole metagenome shotgun sequencing study
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Sohyun Jeong, Yi-Tyng Liao, Min-Hsuan Tsai, Yao-Kuang Wang, I-Chen Wu, Chung-Jung Liu, Ming-Shun Wu, Tze-Sian Chan, Ming-Yao Chen, Ping-Jen Hu, Wei-Yu Kao, Hsiang-Chin Liu, Ming-Ju Tsai, Cheng-Yuan Liu, Chun-Chao Chang, Deng-Chyang Wu, and Yi-Hsiang Hsu
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Fusobacteria ,Bacteroides_caccae ,Bifidobacterium_longum ,Streptococcus_anginosus ,Lachnospiraceae_bacterium_5_1_63FAA ,GLCMANNANAUT-PWY ,Microbiology ,QR1-502 - Abstract
Abstract Background Gastric cancer is one of the global health concerns. A series of studies on the stomach have confirmed the role of the microbiome in shaping gastrointestinal diseases. Delineation of microbiome signatures to distinguish chronic gastritis from gastric cancer will provide a non-invasive preventative and treatment strategy. In this study, we performed whole metagenome shotgun sequencing of fecal samples to enhance the detection of rare bacterial species and increase genome sequence coverage. Additionally, we employed multiple bioinformatics approaches to investigate the potential targets of the microbiome as an indicator of differentiating gastric cancer from chronic gastritis. Results A total of 65 patients were enrolled, comprising 33 individuals with chronic gastritis and 32 with gastric cancer. Within each group, the chronic gastritis group was sub-grouped into intestinal metaplasia (n = 15) and non-intestinal metaplasia (n = 18); the gastric cancer group, early stage (stages 1 and 2, n = 13) and late stage (stages 3 and 4, n = 19) cancer. No significant differences in alpha and beta diversities were detected among the patient groups. However, in a two-group univariate comparison, higher Fusobacteria abundance was identified in phylum; Fusobacteria presented higher abundance in gastric cancer (LDA scored 4.27, q = 0.041 in LEfSe). Age and sex-adjusted MaAsLin and Random Forest variable of importance (VIMP) analysis in species provided meaningful features; Bacteria_caccae was the most contributing species toward gastric cancer and late-stage cancer (beta:2.43, se:0.891, p:0.008, VIMP score:2.543). In contrast, Bifidobacterium_longum significantly contributed to chronic gastritis (beta:-1.8, se:0.699, p:0.009, VIMP score:1.988). Age, sex, and BMI-adjusted MasAsLin on metabolic pathway analysis showed that GLCMANNANAUT-PWY degradation was higher in gastric cancer and one of the contributing species was Fusobacterium_varium. Conclusion Microbiomes belonging to the pathogenic phylum Fusobacteria and species Bacteroides_caccae and Streptococcus_anginosus can be significant targets for monitoring the progression of gastric cancer. Whereas Bifidobacterium_longum and Lachnospiraceae_bacterium_5_1_63FAA might be protection biomarkers against gastric cancer.
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- 2024
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19. The crosstalk between mitochondrial quality control and metal-dependent cell death
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Qi-yuan Zhou, Chao Ren, Jing-yan Li, Lu Wang, Yu Duan, Ren-qi Yao, Ying-ping Tian, and Yong-ming Yao
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Cytology ,QH573-671 - Abstract
Abstract Mitochondria are the centers of energy and material metabolism, and they also serve as the storage and dispatch hubs of metal ions. Damage to mitochondrial structure and function can cause abnormal levels and distribution of metal ions, leading to cell dysfunction and even death. For a long time, mitochondrial quality control pathways such as mitochondrial dynamics and mitophagy have been considered to inhibit metal-induced cell death. However, with the discovery of new metal-dependent cell death including ferroptosis and cuproptosis, increasing evidence shows that there is a complex relationship between mitochondrial quality control and metal-dependent cell death. This article reviews the latest research results and mechanisms of crosstalk between mitochondrial quality control and metal-dependent cell death in recent years, as well as their involvement in neurodegenerative diseases, tumors and other diseases, in order to provide new ideas for the research and treatment of related diseases.
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- 2024
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20. Interferon-α stimulates DExH-box helicase 58 to prevent hepatocyte ferroptosis
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Kai-Wei Jia, Ren-Qi Yao, Yi-Wen Fan, Ding-Ji Zhang, Ye Zhou, Min-Jun Wang, Li-Yuan Zhang, Yue Dong, Zhi-Xuan Li, Su-Yuan Wang, Mu Wang, Yun-Hui Li, Lu-Xin Zhang, Ting Lei, Liang-Chen Gui, Shan Lu, Ying-Yun Yang, Si-Xian Wang, Yi-Zhi Yu, Yong-Ming Yao, and Jin Hou
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Ischemia/reperfusion (I/R) ,DExH-box helicase 58 (DHX58) ,Glutathione peroxidase 4 (GPX4) ,m6A modification ,YT521-B homology domain containing 2 (YTHDC2) ,Medicine (General) ,R5-920 ,Military Science - Abstract
Abstract Background Liver ischemia/reperfusion (I/R) injury is usually caused by hepatic inflow occlusion during liver surgery, and is frequently observed during war wounds and trauma. Hepatocyte ferroptosis plays a critical role in liver I/R injury, however, it remains unclear whether this process is controlled or regulated by members of the DEAD/DExH-box helicase (DDX/DHX) family. Methods The expression of DDX/DHX family members during liver I/R injury was screened using transcriptome analysis. Hepatocyte-specific Dhx58 knockout mice were constructed, and a partial liver I/R operation was performed. Single-cell RNA sequencing (scRNA-seq) in the liver post I/R suggested enhanced ferroptosis by Dhx58 hep−/− . The mRNAs and proteins associated with DExH-box helicase 58 (DHX58) were screened using RNA immunoprecipitation-sequencing (RIP-seq) and IP-mass spectrometry (IP-MS). Results Excessive production of reactive oxygen species (ROS) decreased the expression of the IFN-stimulated gene Dhx58 in hepatocytes and promoted hepatic ferroptosis, while treatment using IFN-α increased DHX58 expression and prevented ferroptosis during liver I/R injury. Mechanistically, DHX58 with RNA-binding activity constitutively associates with the mRNA of glutathione peroxidase 4 (GPX4), a central ferroptosis suppressor, and recruits the m6A reader YT521-B homology domain containing 2 (YTHDC2) to promote the translation of Gpx4 mRNA in an m6A-dependent manner, thus enhancing GPX4 protein levels and preventing hepatic ferroptosis. Conclusions This study provides mechanistic evidence that IFN-α stimulates DHX58 to promote the translation of m6A-modified Gpx4 mRNA, suggesting the potential clinical application of IFN-α in the prevention of hepatic ferroptosis during liver I/R injury.
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- 2024
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21. EFMVFL: An Efficient and Flexible Multi-party Vertical Federated Learning without a Third Party.
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Yimin Huang, Wanwan Wang, Xingying Zhao, Yukun Wang, Xinyu Feng, Hao He, and Ming Yao
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- 2024
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22. Robust Predictive Cascaded Speed and Current Control for PMSM Drives Considering Parameter Variations.
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Xiaodong Sun, Zonghao Su, Gang Lei, and Ming Yao
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- 2024
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23. Model-Free Predictive Current Control for Dual Three-Phase PMSM Drives With an Optimal Modulation Pattern.
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Zonghao Su, Xiaodong Sun, Gang Lei, and Ming Yao
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- 2024
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24. Spatially Averaged Epithelial/Absorbed Power Density for Nonplanar Skin Models Exposed to Antenna at 10-90 GHz.
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Kun Li, Sachiko Kodera, Dragan Poljak, Yinliang Diao, Kensuke Sasaki, Shuai Zhang 0003, Ming Yao, Ante Lojic Kapetanovic, Congsheng Li, Tongning Wu, Thorsten Liebig, Winfried Simon, and Akimasa Hirata
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- 2024
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25. Towards Salomon’s hypothesis via ultra-high-speed cutting Ti-6Al-4V alloy
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Su, Ming-Yao, Wang, De-Ru, Wang, Qi, Jiang, Min-Qiang, and Dai, Lan-Hong
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- 2023
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26. Applying narrative medicine to prepare empathetic healthcare providers in undergraduate pharmacy education in Singapore: a mixed methods study
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Zhe Han, Keith C. Barton, Li-Ching Ho, Kai Zhen Yap, Doreen Su-Yin Tan, Shuh Shing Lee, Constance Xue Rui Neo, Amanda Han Lin Tan, Brian Ming Yao Boey, Charis Jia Yan Soon, and Paul J. Gallagher
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Empathy ,Narrative medicine ,Narratives ,Pharmacy education ,Pharmacist ,Pedagogical design ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Background Narrative medicine demonstrated positive impact on empathy in medicine and nursing students. However, this pedagogical approach had not been evaluated in pharmacy education. This study sought to apply and evaluate the narrative medicine approach in extending empathy in Asian undergraduate pharmacy students. Methods Narrative medicine was applied through workshops which used narratives of people with different experiences and perspectives. First-year undergraduate pharmacy students who volunteered and attended these workshops formed the intervention group (N = 31) and the remaining first-year cohort formed the control group (N = 112). A sequential explanatory mixed methods approach was adopted in which quantitative methods were first used to measure impact on pharmacy students’ empathy using the Jefferson Scale of Empathy– Health Professions Student (JSE-HPS), and qualitative methods (i.e. group interviews) were then used to assess pharmacy students’ emotional responses to narratives, and the perspectives of pharmacy students and faculty of this pedagogical approach. Results There was no difference in JSE-HPS scores between intervention and control groups across baseline (i.e. upon matriculation), pre-intervention, and post-intervention timepoints. Pharmacy students in the intervention group had lower scores in Factor 3 (“Standing in People’s Shoes”) following the intervention. Five themes, guided by internal and external factors in cognition, emerged from the Group Interviews: (1) incongruence between students’ motivation and faculty’s perception, (2) learning context, (3) academic context, (4) cognitive system, and (5) affective system. Themes 1, 4 and 5 referred to internal factors such as students’ motivation, perceived learnings, and feelings. Themes 2 and 3 referred to external factors including workshop materials, activities, content, and facilitation. Conclusion This study is the first to demonstrate that pharmacy students engaged with the narrative medicine approach as narratives elicited emotional responses, exposed them to diverse perspectives, and deepened their appreciation of the importance of empathy and complexities of understanding patients’ perspectives. Scaffolded educational interventions using narratives and real-life patient encounters, alongside longitudinal measurements of empathy, are necessary to bring about meaningful and sustained improvements in empathy.
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- 2024
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27. Endoscopic full-thickness resection using double endoscope–assisted snare traction for a large exophytic gastric subepithelial lesion
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Ding-Ek Toh, MD, Chin-Yuan Yii, MD, Ping-Jen Hu, MD, Bo-Jung Chen, MD, Ming-Yao Chen, MD, Chu-Kuang Chou, MD, and Chung-Ying Lee, MD
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Published
- 2024
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28. Mirrorless MEMS imaging: a nonlinear vibrational approach utilizing aerosol-jetted PZT-actuated fiber MEMS scanner for microscale illumination
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Wei-Chih Wang, Ming-Yao Li, Kuan-Chang Peng, Yi-Feng Hsu, Benjamin Estroff, Pao-Yun Yen, David Schipf, and Wen-Jong Wu
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Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
Abstract This study introduces a novel image capture and lighting techniques using a cutting-edge hybrid MEMS scanner system designed for compact microscopic imaging. The scanner comprises a tapered optical fiber waveguide and innovative aerosol-jet printed PZT (lead zirconate titanate) bimorph push-pull actuators on a stainless-steel substrate, effectively addressing issues that are commonly associated with PZT on silicon substrates such as fracture and layer separation. By leveraging nonlinear vibration, the scanner achieves a spiral scan pattern from a single signal input, in addition to the expected two-dimensional scanning and target illumination from two phase-shifted inputs. This capability is further enhanced by a novel process to taper the optical fiber, which reduces illumination scattering and tunes the fiber to the resonant frequencies of the scanner. The precisely tapered tip enables large fields of view while maintaining independent 2-axis scanning through one-degree-of-freedom actuation. Experimental validation showcases the successful generation of a spiral scan pattern with a 60 μm diameter scan area and a 10 Hz frame rate, effectively reconstructing scanned images of 5 μm lines, cross patterns (15 μm in length with a 5 μm gap), and structures of a Psychodidae wing.
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- 2024
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29. Dysregulated immune and metabolic pathways are associated with poor survival in adult acute myeloid leukemia with CEBPA bZIP in-frame mutations
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Feng-Ming Tien, Chi-Yuan Yao, Xavier Cheng-Hong Tsai, Min-Yen Lo, Chien-Yuan Chen, Wan-Hsuan Lee, Chien-Chin Lin, Yuan-Yeh Kuo, Yen-Ling Peng, Mei-Hsuan Tseng, Yu-Sin Wu, Ming-Chih Liu, Liang-In Lin, Ming-Kai Chuang, Bor-Sheng Ko, Ming Yao, Jih-Luh Tang, Wen-Chien Chou, Hsin-An Hou, and Hwei-Fang Tien
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Acute myeloid leukemia (AML) with CEBPA bZIP in-frame mutations (CEBPA bZIP-inf) is classified within the favorable-risk group by the 2022 European LeukemiaNet (ELN-2022). However, heterogeneous clinical outcomes are still observed in these patients. In this study, we aimed to investigate the mutation profiles and transcriptomic patterns associated with poor outcomes in patients with CEBPA bZIP-inf. One hundred and thirteen CEBPA bZIP-inf patients were identified in a cohort of 887 AML patients homogeneously treated with intensive chemotherapy. Concurrent WT1 or DNMT3A mutations significantly predicted worse survival in AML patients with CEBPA bZIP-inf. RNA-sequencing analysis revealed an enrichment of interferon (IFN) signaling and metabolic pathways in those with a shorter event-free survival (EFS). CEBPA bZIP-inf patients with a shorter EFS had higher expression of IFN-stimulated genes (IRF2, IRF5, OAS2, and IFI35). Genes in mitochondrial complexes I (NDUFA12 and NDUFB6) and V (ATP5PB and ATP5IF1) were overexpressed and were associated with poorer survival, and the results were independently validated in the TARGET AML cohort. In conclusion, concurrent WT1 or DNMT3A mutations and a dysregulated immune and metabolic state were correlated with poor survival in patients with CEBPA bZIP-inf, and upfront allogeneic transplantation may be indicated for better long-term disease control.
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- 2024
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30. Percutaneous Stylomastoid Foramen Pulsed Radiofrequency Combined with Steroid Injection for Treatment of Intractable Facial Paralysis After Herpes Zoster
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Ruyun Deng, Ruxiang Wang, Ming Yao, and Ling Ma
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Facial paralysis ,Herpes zoster ,Pulsed radiofrequency ,Percutaneous stylomastoid foramen ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Introduction We investigated the safety and efficacy of percutaneous facial nerve pulsed radiofrequency combined with drug injection for treatment of intractable facial paralysis of herpes zoster. The authors provide a detailed description of percutaneous facial nerve pulsed radiofrequency combined with steroid injection for treatment of intractable facial paralysis after herpes zoster, and they examine its clinical efficacy. This is the first time in the literature to our knowledge that this procedure has been applied in facial paralysis after herpes zoster. Methods A total of 43 patients with a history of facial paralysis after herpes zoster for > 1 month were enrolled in this retrospective study. The patients were subjected to percutaneous stylomastoid foramen pulsed radiofrequency of the facial nerve under computed tomography (CT) guidance combined with drug injection. The House-Brackmann grades and NRS (Numerical Rating Scale) data collection were performed at different time points (preoperatively, 1 day post-procedure, and 2, 4, and 12 weeks postoperatively). The occurrence of complications was also assessed. Results The 43 participants successfully completed the CT-guided percutaneous stylomastoid foramen pulsed radiofrequency of the facial nerve combined with drug injection. Both approaches [posterior approach of the ear (7 cases) and anterior approach of the ear (36 cases)] were efficacious and safe. The House-Brackmann grades (I, II, III, IV, V, VI) were 4 (3–4), 2 (2–3), 1 (1–2), and 1 (0–2) at different operation times (T0, T1, T2, T3, T4); patients felt significant recovery at T1 after operation and had gradually recovered at each time point but had no significant recovery after T3. The NRS scores at different operation times were 2.690 ± 2.213, 0.700 ± 0.939, 0.580 ± 1.006, 0.440 ± 0.908, and 0.260 ± 0.759, respectively. Differences in NRS scores between T0 and T1/2/3/4 were significant while differences between T1 and T2/3/4 were not significant. Six patients developed mild numbness, nine patients exhibited muscle tension, while one patient exhibited facial stiffness. During surgery, there was no intravascular injection of drugs, no nerve injury was reported, and there was no local anesthetic poisoning or spinal anesthesia. Conclusions Percutaneous stylomastoid foramen pulsed radiofrequency combined with drug injection of the facial nerve for treatment of intractable facial paralysis after herpes zoster is a minimally invasive technique with high rates of success, safety, and effective outcomes. It is a potential therapeutic option for cases of facial paralysis of herpes zoster with a > 1 month history even for those with severe facial paralysis and whose treatment has failed after oral medication and physiotherapy.
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- 2024
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31. Increased serum extrachromosomal circular DNA SORBS1circle level is associated with insulin resistance in patients with newly diagnosed type 2 diabetes mellitus
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Xiang Kong, Shu-jun Wan, Tian-bing Chen, Lan Jiang, Yu-jie Xing, Ya-ping Bai, Qiang Hua, Xin-ming Yao, Yong-li Zhao, Hong-mei Zhang, De-guo Wang, Qing Su, and Kun Lv
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Extrachromosomal circular DNAs ,T2DM ,SORBS1 ,Insulin resistance ,Cytology ,QH573-671 - Abstract
Abstract Background Extrachromosomal circular DNAs (eccDNAs) exist in human blood and somatic cells, and are essential for oncogene plasticity and drug resistance. However, the presence and impact of eccDNAs in type 2 diabetes mellitus (T2DM) remains inadequately understood. Methods We purified and sequenced the serum eccDNAs obtained from newly diagnosed T2DM patients and normal control (NC) subjects using Circle-sequencing. We validated the level of a novel circulating eccDNA named sorbin and SH3‐domain‐ containing‐1circle97206791–97208025 (SORBS1circle) in 106 newly diagnosed T2DM patients. The relationship between eccDNA SORBS1circle and clinical data was analyzed. Furthermore, we explored the source and expression level of eccDNA SORBS1circle in the high glucose and palmitate (HG/PA)-induced hepatocyte (HepG2 cell) insulin resistance model. Results A total of 22,543 and 19,195 eccDNAs were found in serum samples obtained from newly diagnosed T2DM patients and NC subjects, respectively. The T2DM patients had a greater distribution of eccDNA on chromosomes 1, 14, 16, 17, 18, 19, 20 and X. Additionally, 598 serum eccDNAs were found to be upregulated, while 856 eccDNAs were downregulated in T2DM patients compared with NC subjects. KEGG analysis demonstrated that the genes carried by eccDNAs were mainly associated with insulin resistance. Moreover, it was validated that the eccDNA SORBS1circle was significantly increased in serum of newly diagnosed T2DM patients (106 T2DM patients vs. 40 NC subjects). The serum eccDNA SORBS1circle content was positively correlated with the levels of glycosylated hemoglobin A1C (HbA1C) and homeostasis model assessment of insulin resistance (HOMA-IR) in T2DM patients. Intracellular eccDNA SORBS1circle expression was significantly enhanced in the high glucose and palmitate (HG/PA)-induced hepatocyte (HepG2 cell) insulin resistance model. Moreover, the upregulation of eccDNA SORBS1circle in the HG/PA-treated HepG2 cells was dependent on generation of apoptotic DNA fragmentation. Conclusions These results provide a preliminary understanding of the circulating eccDNA patterns at the early stage of T2DM and suggest that eccDNA SORBS1circle may be involved in the development of insulin resistance.
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- 2024
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32. N4-acetylcytidine acetylation of neurexin 2 in the spinal dorsal horn regulates hypersensitivity in a rat model of cancer-induced bone pain
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Longsheng Xu, Shang Zheng, Liping Chen, Lei Yang, Shuyao Zhang, Beibei Liu, Kangli Shen, Qinli Feng, Qinghe Zhou, and Ming Yao
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MT: RNA and epigenetic editing Special Issue ,cancer-induced bone pain ,neurexin 2 ,RNA acetylation ,N-acetyltransferase 10 ,synaptic remodeling ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Cancer-induced bone pain (CIBP) significantly impacts the quality of life and survival of patients with advanced cancer. Despite the established role of neurexins in synaptic structure and function, their involvement in sensory processing during injury has not been extensively studied. In this study using a rat model of CIBP, we observed increased neurexin 2 expression in spinal cord neurons. Knockdown of neurexin 2 in the spinal cord reversed CIBP-related behaviors, sensitization of spinal c-Fos neurons, and pain-related negative emotional behaviors. Additionally, increased acetylation of neurexin 2 mRNA was identified in the spinal dorsal horn of CIBP rats. Decreasing the expression of N-acetyltransferase 10 (NAT10) reduced neurexin 2 mRNA acetylation and neurexin 2 expression. In PC12 cells, we confirmed that neurexin 2 mRNA acetylation enhanced its stability, and neurexin 2 expression was regulated by NAT10. Finally, we discovered that the NAT10/ac4C-neurexin 2 axis modulated neuronal synaptogenesis. This study demonstrated that the NAT10/ac4C-mediated posttranscriptional modulation of neurexin 2 expression led to the remodeling of spinal synapses and the development of conscious hypersensitivity in CIBP rats. Therefore, targeting the epigenetic modification of neurexin 2 mRNA ac4C may offer a new therapeutic approach for the treatment of nociceptive hypersensitivity in CIBP.
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- 2024
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33. Evaluation of Methods to Obtain Peripheral Blood Mononuclear Cells From Deceased Donors for Tolerance-Induction Protocols
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Ming Yao, Jarmo Henriksson, Henrik Fahlander, Pablo Guisti Coitinho, Torbjörn Lundgren, Nils Ågren, Bo-Göran Ericzon, and Makiko Kumagai-Braesch
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Medicine - Abstract
Regulatory cell therapies have shown promise in tolerance-induction protocols in living donor organ transplantation. These protocols should be pursued in deceased donor transplantation. Donor peripheral mononuclear cells (PBMCs) are an optimal source of donor antigens for the induction of donor-specific regulatory cells. During the development of a regulatory cell tolerance-induction protocol with organs from deceased donors, we compared 3 methods of obtaining PBMCs from deceased donors focusing on cell yield, viability, and contamination of unwanted cell types. PBMC procurement methods: 1. During organ procurement at the time of cold perfusion, blood was collected from the vena cava and placed into a 10-liter blood collection bag, and thereafter transported to Karolinska University Hospital, where leukapheresis was performed (BCL). 2. Blood was collected via the vena cava into blood donation bags before cold perfusion. The bags underwent buffy coat separation and thereafter automated leukocyte isolation system (BCS). 3. To collect PBMCs, leukapheresis was performed via a central dialysis catheter on deceased donors in the intensive care unit (ICU) prior to the organ procurement procedure (LEU). All 3 methods to obtain PBMC from deceased donors were safe and did not affect the procurement of organs. BCL contained around 50% of NK cells in lymphocytes population. LEU had a highest yield of donor PBMC among 3 groups. LEU had the lower amount of granulocyte contamination, compared to BCS and BCL. Based on these results, we choose LEU as the preferred method to obtain donor PBMC in the development of our tolerance-induction protocol.
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- 2024
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34. Dynamic Mechanism of Cerebral Venous Disruption: Longitudinal Evidence From a Community‐Based Cohort
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Zi‐Yue Liu, Pei Wang, Fei‐Fei Zhai, Dong‐Hui Ao, Fei Han, Ming‐Li Li, Li‐Xin Zhou, Jun Ni, Ming Yao, Shu‐Yang Zhang, Li‐Ying Cui, Zheng‐Yu Jin, and Yi‐Cheng Zhu
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brain atrophy ,deep medullary veins ,longitudinal study ,neurodegenerative disease ,white matter microstructure ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background This study aims to investigate the temporal and spatial patterns of structural brain injury related to deep medullary veins (DMVs) damage. Methods and Results This is a longitudinal analysis of the population‐based Shunyi cohort study. Baseline DMVs numbers were identified on susceptibility‐weighted imaging. We assessed vertex‐wise cortex maps and diffusion maps at both baseline and follow‐up using FSL software and the longitudinal FreeSurfer analysis suite. We performed statistical analysis of global measurements and voxel/vertex‐wise analysis to explore the relationship between DMVs number and brain structural measurements. A total of 977 participants were included in the baseline, of whom 544 completed the follow‐up magnetic resonance imaging (age 54.97±7.83 years, 32% men, mean interval 5.56±0.47 years). A lower number of DMVs was associated with a faster disruption of white matter microstructural integrity, presented by increased mean diffusivity and radial diffusion (β=0.0001 and SE=0.0001 for both, P=0.04 and 0.03, respectively), in extensive deep white matter (threshold‐free cluster enhancement P
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- 2024
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35. Hydroxychloroquine-induced hyperpigmentation of the skin and bull’s-eye maculopathy in rheumatic patients: a case report and literature review
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Ji-peng Peng, Xiao-yu Yang, Feng Luo, Xue-mei Yuan, Hong Xiong, Wu-kai Ma, and Xue-ming Yao
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hydroxychloroquine ,adverse drug reaction ,hyperpigmentation of the skin ,bull’s-eye maculopathy ,literature review ,autoimmune disease ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Hydroxychloroquine (HCQ) is used as a traditional disease-modifying antirheumatic drugs (DMARDs), for the treatment of autoimmune diseases such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). However, it can cause serious adverse reactions, including hyperpigmentation of the skin and bull’s-eye macular lesions. Here, we present a case of HCQ-induced hyperpigmentation of the skin and bull’s-eye macular lesions in a patient who received HCQ for RA. A 65-year-old female patient developed blurred vision and hyperpigmentation of multiple areas of skin over the body for one month after 3 years of HCQ treatment for RA. Based on clinical presentation, ophthalmological examination and dermatopathological biopsy, a diagnosis of drug-induced cutaneous hyperpigmentation and bullous maculopathy of the right eye was made. After discontinuation of HCQ and treatment with iguratimod tablets, the hyperpigmentation of the patient ‘s skin was gradually reduced, and the symptoms of blurred vision were not significantly improved. We also reviewed the available literature on HCQ-induced cutaneous hyperpigmentation and bull’s-eye macular lesions and described the clinical features of HCQ-induced cutaneous hyperpigmentation and bull’s-eye macular lesions. In conclusion, clinicians should be aware of early cutaneous symptoms and HCQ-associated ophthalmotoxicity in patients with rheumatic diseases on HCQ sulphate and should actively monitor patients, have them undergo regular ophthalmological examinations and give appropriate treatment to prevent exacerbation of symptoms.
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- 2024
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36. The role of orphan G protein-coupled receptors in pain
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Chengfei Xu, Yahui Wang, Huadong Ni, Ming Yao, Liang Cheng, and Xuewu Lin
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G protein-coupled receptor ,Orphan G protein-coupled receptor ,Pain ,Endogenous ligand ,Monoclonal antibody ,Rodents ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
G protein-coupled receptors (GPCRs), which form the largest family of membrane protein receptors in humans, are highly complex signaling systems with intricate structures and dynamic conformations and locations. Among these receptors, a specific subset is referred to as orphan GPCRs (oGPCRs) and has garnered significant interest in pain research due to their role in both central and peripheral nervous system function. The diversity of GPCR functions is attributed to multiple factors, including allosteric modulators, signaling bias, oligomerization, constitutive signaling, and compartmentalized signaling. This review primarily focuses on the recent advances in oGPCR research on pain mechanisms, discussing the role of specific oGPCRs including GPR34, GPR37, GPR65, GPR83, GPR84, GPR85, GPR132, GPR151, GPR160, GPR171, GPR177, and GPR183. The orphan receptors among these receptors associated with central nervous system diseases are also briefly described. Understanding the functions of these oGPCRs can contribute not only to a deeper understanding of pain mechanisms but also offer a reference for discovering new targets for pain treatment.
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- 2024
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37. Acculturating again: Taiwanese migrants' enduring COVID-19 coping paradox in the UK
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Yen, Dorothy Ai-wan, Cappellini, Benedetta, Hendy, Jane Denise, and Jen, Ming-Yao
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- 2023
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38. Correction: Involvement of FSP1-CoQ10-NADH and GSH-GPx-4 pathways in retinal pigment epithelium ferroptosis
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Ming Yang, Michelle Grace Tsui, Jessica Kwan Wun Tsang, Rajesh Kumar Goit, Kwok-Ming Yao, Kwok-Fai So, Wai-Ching Lam, and Amy Cheuk Yin Lo
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Cytology ,QH573-671 - Published
- 2024
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39. Targeting the Neuropilin-1 receptor with Ovatodiolide and progress in using periodontal ligament organoids for COVID-19 research and therapy
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Hsieh, Ming-Shou, Chen, Ming-Yao, Chang, Yu-Sheng, Huang, Chin-Sheng, Hsu, Tung-Nien, Huang, Mao-Suan, Yeh, Chi-Tai, and Tzeng, Yew-Min
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- 2024
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40. Effect of CT-Guided Repeated Pulsed Radiofrequency on Controlling Acute/Subacute Zoster-Associated Pain: A Retrospective Cohort Study
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Min Rui, Zixin Han, Longsheng Xu, and Ming Yao
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Herpes zoster ,Pulsed radiofrequency treatment ,Paravertebral block ,Dorsal root ganglion ,Postherpetic neuralgia ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Introduction Zoster-associated pain (ZAP) treatment and management is still inadequate. Repeated intervention protocol is often applied to manage ZAP. This study aimed to retrospectively investigate the effect of repeated applications of pulsed radiofrequency therapy on controlling acute/subacute ZAP. Methods From March 2019 to December 2021, 150 patients with acute/subacute ZAP who underwent repeated application of pulsed radiofrequency treatment (R-PRF) and pulsed radiofrequency combined paravertebral block interventions (PRF + PVB) in the Pain Department of the affiliated Hospital of Jiaxing University were enrolled. Patients were grouped by intervention protocol and received at least 12 months of follow-up assessments using the Numerical Rating Scale score (NRSs), Pittsburg Sleep Quality Index (PSQI), Simple McGill Pain Questionnaire-2 score (SF-MPQ-2s), and follow-up interventions. Results Both groups experienced a reduction in the incidence of clinically meaningful ZAP after the intervention therapy. In the R-PRF group, there were 36 cases of clinically meaningful ZAP within the first month post-treatment, while the PRF + PVB group had 38 cases. The incidence of clinically meaningful ZAP, as determined by multivariable generalized estimating equations, was 42.86% in the R-PRF group and 57.58% in the PRF + PVB group during the first month of follow-up. There was a significant difference in the incidence of clinically meaningful ZAP between the two groups after 1 month of treatment (adjusted odds ratio: 0.40; 95% confidence interval: 0.18–0.91; p = 0.03). Conclusions Both R-PRF and PRF + PVB treatments effectively relieve pain in patients with acute/subacute ZAP. However, R-PRF may have superior efficacy compared to PRF + PVB in reducing the incidence of clinically meaningful ZAP 1 month after treatment.
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- 2023
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41. Allogeneic hematopoietic stem cell transplantation for B‐cell lymphoma in Taiwan
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Chun‐Hui Lee, Tzu‐Chien Lin, Ming Yao, Liang‐Tsai Hsiao, Bor‐Sheng Ko, Chia‐Jen Liu, and Tsai‐Yun Chen
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B‐cell lymphoma ,complete remission ,diffuse large B‐cell lymphoma ,hematopoietic stem cell transplantation ,relapse and refractory ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is considered for patients with high‐risk B‐cell lymphoma and relapsed or refractory disease. This study aimed to analyze the long‐term follow‐up data of patients who underwent allo‐HSCT in Taiwan. This was a retrospective observational study using data from the Taiwan Society of Blood and Marrow Transplantation database. A total of 105 patients who underwent allo‐HSCT because of high‐risk, relapsed, or refractory disease between 2010 and 2019 were included. Forty‐five percent of the patients previously underwent autologous stem cell transplantation (ASCT). The median follow‐up duration was 18.6 months. The probability of 3‐year progression‐free survival and overall survival (OS) was 34.5% and 37%, respectively. The probability of 1‐year non‐relapse mortality was 31.4%, and the major cause was infection (75.8%). The multivariable analysis showed that not in remission at the time of transplantation and the absence of graft‐versus‐host disease (GVHD) were factors associated with inferior OS. The probability of 3‐year OS in patients with diffuse large B‐cell lymphoma who underwent allo‐HSCT and allo‐HSCT after ASCT was 40.2% and 25.2%, respectively. Allo‐HSCT could be a salvage therapeutic option for relapsed or refractory B‐cell lymphoma. Complete remission at the time of allo‐HSCT and the presence of GVHD are independent variables for overall survival.
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- 2023
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42. Progress in Radiofrequency Therapy for Zoster-Associated Pain About Parameters, Modes, Targets, and Combined Therapy: A Narrative Review
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Min Rui, Huadong Ni, Keyue Xie, Longsheng Xu, and Ming Yao
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Herpes zoster ,Postherpetic neuralgia ,Pulsed radiofrequency therapy ,Dorsal root ganglion ,Intercostal nerve ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Zoster-associated pain (ZAP) is a painful condition that significantly impacts a patient’s quality of life, often leading to postherpetic neuralgia (PHN). Over 30% of patients with herpes probably experience PHN. However, the understanding and treatment of ZAP remain inadequate. Common interventional treatments include radiofrequency therapy, nerve blocks, epidural block, and spinal cord electrical stimulation. Among these, radiofrequency therapy is widely used for pain control in ZAP, but the standard pulsed radiofrequency technique can still be improved. Researchers have explored different radiofrequency parameters, modes, targets, and combined treatments to enhance the therapeutic effect. In this paper, we review the latest research findings and incorporate our own departmental investigations. We conclude that high-voltage, long-duration pulsed radiofrequency and radiofrequency thermocoagulation therapy have shown improved therapeutic outcomes, despite some remaining limitations. Emphasis is placed on safety in intercostal nerve and extracranial nerve radiofrequency treatments. Combination therapy is also safe and effective; however, many studies have a low grade of evidence. Further high-quality research and systematic reviews are needed.
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- 2023
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43. Phenotypes Associated with NOTCH3 Cysteine-Sparing Mutations in Patients with Clinical Suspicion of CADASIL: A Systematic Review
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Yuan Cao, Ding-Ding Zhang, Fei Han, Nan Jiang, Ming Yao, and Yi-Cheng Zhu
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CADASIL ,NOTCH3 ,cysteine-sparing ,clinical phenotype ,radiological cerebral phenotype ,systematic review ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy) is caused by NOTCH3 mutations affecting the number of cysteines. The pathogenic role of cysteine-sparing NOTCH3 mutations with typical clinical CADASIL syndrome is still debated. This review aimed to characterize NOTCH3 cysteine-sparing mutations in patients with clinical suspicion of CADASIL. Articles on NOTCH3 cysteine-sparing mutations with clinical suspicion of CADASIL were reviewed. Clinical and radiological cerebral phenotypes data were extracted and characterized across regions and compared with phenotypes of typical CADASIL patients. We screened 298 NOTCH3 cysteine-sparing mutation individuals from 20 publications, and mutations in exon 3 were the most frequently reported (21.46%). Gait impairment (76.47%), cognitive impairment (67.47%), and stroke (62.37%) were the three most common clinical phenotypes; the most frequent radiological cerebral phenotypes were lacunes (74.29%) and cerebral microbleeds (72.73%). Compared with CADASIL patients, cognitive impairment and cerebral microbleed frequencies were significantly higher in patients with NOTCH3 cysteine-sparing mutations, while the white matter hyperintensities in anterior temporal polar and external capsule were rarely observed. Compared with Western patients, radiological phenotypes were more common than clinical phenotypes in cysteine-sparing Asian patients. More than half of cysteine-sparing patients had positive granular osmiophilic material deposits. NOTCH3 cysteine-sparing mutations in patients with clinical suspicion of CADASIL mainly manifested with gait and cognitive impairment but rare white matter hyperintensities in anterior temporal pole and external capsule. Further studies are warranted to pay attention to atypical NOTCH3 variants, which could guide specific diagnosis and help unravel underlying mechanisms.
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- 2024
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44. Milk Fat Globule-EGF Factor 8 (MFGE8) Mitigates Cognitive Impairment in Rats with Sepsis-Associated Encephalopathy: An fMRI Study
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Haojia Li, Hongsen Liao, Lu Sun, Dejian Chen, Yong Fei, Ming Yao, Bing Huang, Xiaoyan Guo, Shaozheng Song, and Hongguang Bao
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microglial efferocytosis ,sepsis associated encephalopathy ,regional spontaneous neuronal activity ,milk fat globule egf factor 8 protein ,functional magnetic resonance imaging ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Background: Sepsis-associated encephalopathy (SAE) impairs hippocampal microglial efferocytosis, causing cognitive deficits. Previous research found that milk fat globule epidermal growth factor 8 protein (MFGE8) stimulates efferocytosis, reducing hippocampal inflammation in SAE rats. In this study, we explore MFGE8’s role in alleviating cognitive impairment and its impact on neural activity using functional magnetic resonance imaging (fMRI). Methods: Sixty male Sprague Dawley rats were divided into four groups: Sham, cecal ligation and puncture (CLP), CLP+MFGE8, and CLP+MFGE8+CGT (Cilengitide). After CLP, CLP+MFGE8 rats received intracerebroventricular MFGE8 (3.3 µg), while CLP+MFGE8+CGT rats received intraperitoneal Cilengitide (10 mg/kg). We assessed cognitive function with the Morris water maze and open field test over five days. Eight days post-surgery, rats underwent T2-weighted magnetic resonance imaging (MRI) and resting state (rs)-fMRI scans. Brain tissues were collected for western blot, hematoxylin-eosin (HE) staining, and immunofluorescence. Statistical analysis employed one-way analysis of variance (ANOVA) followed by Tukey’s post-test for multiple comparisons. Results: MFGE8 improved neurobehavioral performance in open field task (OFT) and morris water maze (MWM) tests. fMRI indicated a significant reduction in abnormal neural activity in the right hippocampal CA1, CA3, and dentate gyrus of SAE rats following MFGE8 treatment. Voxel-based morphometry (VBM) analysis revealed decreased high-signal areas in the hippocampus, along with reduced hippocampal volume due to alleviated neural edema. Western blot analysis demonstrated that MFGE8 enhanced ras-related C3 botulinum toxin substrate 1 (Rac1) and microtubule-associated protein 1A/1B-light chain 3 (LC3) expression in the rat hippocampus, while CGT reduced these protein levels. Behavioral experiments and fMRI results confirmed that CGT reversed the cognitive effects of MFGE8 by inhibiting microglial αVβ3/αVβ5 integrin receptors. Conclusions: Our findings show that MFGE8 reduced amplitude of low-frequency fluctuations (ALFF) values in the right hippocampal CA1, CA3, and the dentate gyrus, mitigating abnormal neural activity and decreasing hippocampal volume. This led to an improvement in cognitive dysfunction in SAE rats. These results suggest that MFGE8 enhances microglial efferocytosis by activating αVβ3 and αVβ5 integrin receptors on microglial surfaces, ultimately improving cognitive function in SAE rats.
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- 2024
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45. Health economic evaluation of an artificial intelligence (AI)-based rapid nutritional diagnostic system for hospitalised patients: A multicentre, randomised controlled trial
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Sun, Ming-Yao, Wang, Yu, Zheng, Tian, Wang, Xue, Lin, Fan, Zheng, Lu-Yan, Wang, Mao-Yue, Zhang, Pian-Hong, Chen, Lu-Ying, Yao, Ying, Sun, Jie, Li, Zeng-Ning, Hu, Huan-Yu, Jiang, Hua, Yue, Han-Yang, Zhao, Qian, Wang, Hai-Yan, Han, Lei, Ma, Xuan, Ji, Meng-Ting, Xu, Hong-Xia, Luo, Si-Yu, Liu, Ying-Hua, Zhang, Yong, Han, Ting, Li, Yan-Sheng, Hou, Peng-Peng, and Chen, Wei
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- 2024
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46. Development and Testing of a Novel Large Language Model-Based Clinical Decision Support Systems for Medication Safety in 12 Clinical Specialties.
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Jasmine Chiat Ling Ong, Liyuan Jin, Kabilan Elangovan, Gilbert Yong San Lim, Daniel Yan Zheng Lim, Gerald Gui Ren Sng, Yuhe Ke, Joshua Yi Min Tung, Ryan Jian Zhong, Christopher Ming Yao Koh, Keane Zhi Hao Lee, Xiang Chen, Jack Kian Chng, Aung Than, Ken Junyang Goh, and Daniel Shu Wei Ting
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- 2024
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47. Health financing challenges in Southeast Asian countries for universal health coverage: a systematic review
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Lim, Ming Yao, Kamaruzaman, Hanin Farhana, Wu, Olivia, and Geue, Claudia
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- 2023
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48. Endovascular aortic arch repair with chimney technique for pseudoaneurysm
- Author
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Luo, Ming-yao, Zhang, Xiong, Fang, Kun, Guo, Yuan-yuan, Chen, Dong, Lee, Jason T., and Shu, Chang
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- 2023
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49. Association of Rare NOTCH3 Variants With Prevalent and Incident Stroke and Dementia in the General Population
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Pei Wang, Ming Yao, Jing Yuan, Fei Han, Fei‐Fei Zhai, Ding‐Ding Zhang, Li‐Xin Zhou, Jun Ni, Shu‐Yang Zhang, Li‐Ying Cui, and Yi‐Cheng Zhu
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cognitive function ,dementia ,NOTCH3 ,rare variants ,stroke ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background It is uncertain whether rare NOTCH3 variants are associated with stroke and dementia in the general population and whether they lead to alterations in cognitive function. This study aims to determine the associations of rare NOTCH3 variants with prevalent and incident stroke and dementia, as well as cognitive function changes. Methods and Results In the prospective community‐based Shunyi Study, a total of 1007 participants were included in the baseline analysis. For the follow‐up analysis, 1007 participants were included in the stroke analysis, and 870 participants in the dementia analysis. All participants underwent baseline brain magnetic resonance imaging, carotid ultrasound, and whole exome sequencing. Rare NOTCH3 variants were defined as variants with minor allele frequency
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- 2024
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50. Impact of early heparin therapy on mortality in critically ill patients with sepsis associated acute kidney injury: a retrospective study from the MIMIC-IV database
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Zhi-Peng Zhou, Li Zhong, Yan Liu, Zhen-Jia Yang, Jia-Jia Huang, Da-Zheng Li, Yu-Hua Chen, Ying-Yi Luan, Yong-Ming Yao, and Ming Wu
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heparin ,sepsis-associated acute kidney injury ,outcome ,mortality ,marginal structural Cox model ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Inflammatory-coagulation dysfunction plays an increasingly important role in sepsis associated acute kidney injury (SAKI). This study aimed to investigate whether early heparin therapy improves survival in patients with SAKI.Methods: Patients with SAKI were identified from the Medical Information Mart for Intensive Care-IV database. The patients were divided into two groups: those who received heparin subcutaneously within 48 h after intensive care unit (ICU) admission and the control group, who received no heparin. The primary endpoint was ICU mortality, the secondary outcomes were 7-day, 14-day, 28-day, and hospital mortality. Propensity score matching (PSM), marginal structural Cox model (MSCM), and E-value analyses were performed.Results: The study included 5623 individuals with SAKI, 2410 of whom received heparin and 3213 of whom did not. There were significant effects on ICU and 28-day mortality in the overall population with PSM. MSCM further reinforces the efficacy of heparin administration reduces ICU mortality in the general population. Stratification analysis with MSCM showed that heparin administration was associated with decreased ICU mortality at various AKI stages. Heparin use was also associated with reduced 28-day mortality in patients with only female, age >60 years, and AKI stage 3, with HRs of 0.79, 0.77, and 0.60, respectively (p < 0.05). E-value analysis suggests robustness to unmeasured confounding.Conclusion: Early heparin therapy for patients with SAKI decreased ICU mortality. Further analysis demonstrated that heparin therapy was associated with reduced 28-day mortality rate in patients only among female, age > 60 years and AKI stage 3.
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- 2024
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