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The zonal flow and volume transport in the Luzon Strait play a crucial role in modulating the circulation, heat and material balance, and biogeochemical processes in the South China Sea (SCS), but the quantitative values remain unclear due to lack of long‐term direct observations. Here, this knowledge gap is bridged by analyzing 4‐year velocity measurements from a mooring array along 119.9°E. Based on the novel data, the mean value of the upper‐layer Luzon Strait transport (i.e., LST_up) is estimated at −4.54 ± 1.69 Sv. Seasonally, the westward LST_up attains its peak and trough in January and June with values of −6.80 ± 1.46 Sv and −2.59 ± 0.76 Sv, respectively. At the interannual time scale, the LST_up was strongest in 2016–2017 but weakest in 2017–2018. Further analysis suggested that local winds and the combination of local winds and upstream Kuroshio transport are likely the dominant modulation mechanisms for its seasonal and interannual variations, respectively. In the middle layer, a quasi‐steady cyclonic flow structure is identified and the volume transport is therefore trivial. We further found that seasonal variation of the middle‐layer transport is dominated by the eastward flow of this cyclonic structure. Corresponding to the gravest‐mode response in the Luzon Strait, seasonal‐to‐interannual variations of this middle‐layer eastward flow are nearly in‐phase with the upper‐layer westward flow. Overall, the above observational results provide a benchmark for the flow and transport in the Luzon Strait which can help understand the dynamics of the SCS circulation and validate and improve regional numerical simulations. Plain Language Summary: The Luzon Strait is the main channel connecting the South China Sea (SCS) with the Pacific Ocean and the volume transport through the strait is crucial in regulating physical and biogeochemical processes in the SCS. However, given the sparsity of long‐term direct observations, the knowledge of zonal flow and transports is still poor. In this study, novel insights into the zonal flow and transport in the Luzon Strait are obtained based on 4‐year velocity measurements from six moorings. The upper‐layer Luzon Strait transport is estimated at −4.54 ± 1.69 Sv (1 Sv = 106 m3 s−1; negative denotes westward). The flow in the middle layer presents a relatively steady cyclonic structure and the transport through the observed section is trivial because the westward and eastward flows cancel each other out. Seasonal‐to‐interannual variations of the transports are also analyzed. Further studies revealed that the middle‐layer eastward flow shows nearly the same phase with the upper‐layer westward flow at seasonal‐to‐interannual time scales. This corresponds well to the gravest‐mode response to external forcings. The present observed results will provide a benchmark for better understanding the dynamics of the SCS circulation and can be used to validate and improve numerical simulations in this region. Key Points: A quasi‐steady cyclonic flow structure is revealed in the middle layer of Luzon Strait based on 4‐year mooring observationsThe upper‐layer Luzon Strait transport is estimated at −4.54 ± 1.69 Sv while the middle‐layer transport is trivialThe upper‐layer westward flow and middle‐layer eastward flow show in‐phase temporal variations corresponding to the gravest‐mode response [ABSTRACT FROM AUTHOR]

In this paper, a new computation scheme based on parallelization is proposed for Isogeometric analysis. The parallel computing is introduced to the whole progress of Isogeometric analysis. Firstly, with the help of the “tensor-product” and “iso-parametric” feature, all the Gaussian integral points in particular element can be mapped to a global matrix using a transformation matrix that varies from element. Then the derivatives of Gauss integral points are computed in parallel, the results of which can be stored in a global matrix. And a middle layer is constructed to assemble the final stiffness matrices in parallel. The numerical example results show that: the method presented in the paper can reduce calculation time and improve the use rate of computing resources. [ABSTRACT FROM AUTHOR]

Despite clear evidence that a local accumulation of auxin is likewise critical for male fertility, much less is known about the components that regulate auxin-controlled stamen development. In this study, we analyzed physiological and morphological parameters in mutants of key players of ABCB-mediated auxin transport, and spatially and temporally dissected their expression on the protein level as well as auxin fluxes in the Arabidopsis stamens. Our analyses revealed that the FKBP42, TWISTED DWARF1 (TWD1), promotes stamen elongation and, to a lesser extent, anther dehiscence, as well as pollen maturation, and thus is required for seed development. Most of the described developmental defects in twd1 are shared with the abcb1 abcb19 mutant, which can be attributed to the fact that TWD1—as a described ABCB chaperone—is a positive regulator of ABCB1- and ABCB19-mediated auxin transport. However, reduced stamen number was dependent on TWD1 but not on investigated ABCBs, suggesting additional players downstream of TWD1. We predict an overall housekeeping function for ABCB1 during earlier stages, while ABCB19 seems to be responsible for the key event of rapid elongation at later stages of stamen development. Our data indicate that TWD1 controls stamen development by differential activation of ABCB1,19-mediated auxin transport in the stamen. [ABSTRACT FROM AUTHOR]

The surface of pollen grains is reinforced by pollen wall components produced noncell autonomously by tapetum cells that surround developing pollen within the male floral organ, the anther. Here, we show that tapetum activity is regulated by the GASSHO (GSO) receptor-like kinase pathway, controlled by two sulfated peptides, CASPARIAN STRIP INTEGRITY FACTOR 3 (CIF3) and CIF4, the precursors of which are expressed in the tapetum itself. Coordination of tapetum activity with pollen grain development depends on the action of subtilases, including AtSBT5.4, which are produced stage specifically by developing pollen grains. Tapetum-derived CIF precursors are processed by subtilases, triggering GSO-dependent tapetum activation. We show that the GSO receptors act from the middle layer, a tissue surrounding the tapetum and developing pollen. Three concentrically organized cell types, therefore, cooperate to coordinate pollen wall deposition through a multilateral molecular dialogue. [ABSTRACT FROM AUTHOR]

An approximate model of the process of loss in carrying capacity of the three-layered rod under tension is proposed. The criterion of failure is a loss in stability of the middle layer. The critical load is calculated according to the classical expression for it in a problem of the compression of the rod in an elastic medium. A technique has been developed to determine the coefficient of subgrade reaction for the middle layer based on a comparison of analytical and numerical solutions to the problem of bending a beam on an elastic foundation with known geometric and mechanical parameters (a two-dimensional finite-element model is used for the numerical solution). On the basis of the performed calculations, the regularities of the change in the coefficient of subgrade reaction are revealed depending on a variation of the geometric and mechanical parameters; these regularities are presented in a form of the regression function. Some dependences of the critical load on these parameters are obtained. [ABSTRACT FROM AUTHOR]

The middle layer is an essential cell layer of the anther wall located between the endothecium and tapetum in Arabidopsis. Based on sectioning, the middle layer was found to be degraded at stage 7, which led to the separation of the tapetum from the anther wall. Here, we established techniques for live imaging of the anther. We created a marker line with fluorescent proteins expressed in all anther layers to study anther development. Several staining methods were used in the intact anthers to study anther cell morphology. We clarified the initiation, development, and degradation of the middle layer in Arabidopsis. This layer is initiated from both the inner and outer secondary parietal cells at stage 4, stopped cell division at stage 6, and finally degraded at stage 11. The neighboring cell layers, the epidermis, and endothecium continued cell division until stage 10, which led to a thin middle layer. The degradation of the tapetum cell wall at stage 7 lead to its isolation from the anther wall. This work presents fundamental information on the development of the middle layer, which facilitates the further investigation of anther development and plant fertility. These live imaging methods could be useful in future studies.

The middle layer is an essential cell layer of the anther wall located between the endothecium and tapetum in Arabidopsis. Based on sectioning, the middle layer was found to be degraded at stage 7, which led to the separation of the tapetum from the anther wall. Here, we established techniques for live imaging of the anther. We created a marker line with fluorescent proteins expressed in all anther layers to study anther development. Several staining methods were used in the intact anthers to study anther cell morphology. We clarified the initiation, development, and degradation of the middle layer in Arabidopsis. This layer is initiated from both the inner and outer secondary parietal cells at stage 4, stopped cell division at stage 6, and finally degraded at stage 11. The neighboring cell layers, the epidermis, and endothecium continued cell division until stage 10, which led to a thin middle layer. The degradation of the tapetum cell wall at stage 7 lead to its isolation from the anther wall. This work presents fundamental information on the development of the middle layer, which facilitates the further investigation of anther development and plant fertility. These live imaging methods could be useful in future studies. [ABSTRACT FROM AUTHOR]

Copyright of Orbis Scholae is the property of Charles University Prague, Faculty of Education and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guangdong Architecture Civil Engineering is the property of Guangdong Architecture Civil Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Autotetraploid rice is a useful rice germplasm for polyploid rice breeding. However, low fertility limits its commercial production. A neo-tetraploid rice with high fertility was developed from the progenies of crossing between autotetraploid lines by our research group. Our previous study showed that a myeloblastosis (MYB) transcription factor, MOF1, might be associated with the pollen development in tetraploid rice. However, little information is available about its role in pollen development in tetraploid rice. Here, we identified a new haplotype of MOF1 from neo-tetraploid rice and marked it as MOF1a. Transcriptome and qRT-PCR analysis demonstrated that MOF1a highly expressed in anthers, and displayed differential expression in neo-tetraploid rice compared to tetraploid rice line with low pollen fertility. The mutant (mof1a) of MOF1a, which was generated by CRISPR/Cas9 knockout in neo-tetraploid rice, showed low pollen fertility, and also exhibited abnormal tapetum and middle layer development, and defective chromosome behaviors during meiosis. A total of 13 tapetal related genes were found to be up-regulated in meiotic anthers of MOF1a compared with wild type plants by RNA-seq analysis, including CYP703A3, PTC1, and OsABCG26, which had been demonstrated to affect tapetal development. Moreover, 335 meiosis-related genes displayed differential expression patterns at same stage, including nine important meiosis-related genes, such as metallothionein OsMT1a. These results demonstrated that MOF1a plays an important role in pollen development and provides a foundation for understanding the molecular mechanism underlying MOF1a in reproduction of tetraploid rice.

• Here, we investigated the role of auxin distribution in controlling Arabidopsis thaliana late stamen development. • We analysed auxin distribution in anthers by monitoring DR5 activity: at different flower developmental stages; inhibiting auxin transport; in the rpk2-3 and ems1 mutants devoid of middle layer (ML) or tapetum, respectively; and in the auxin biosynthesis yuc6 and perception afb1-3 mutants. We ran a phenotypic, DR5::GUS and gene expression analysis of yuc6rpk2 and afb1rpk2 double mutants, and of 1-N-naphthylphthalamic acid (NPA)-treated flower buds. • We show that an auxin maximum, caused by transport from the tapetum, is established in the ML at the inception of late stamen development. rpk2-3 mutant stamens lacking the ML have an altered auxin distribution with excessive accumulation in adjacent tissues, causing non-functional pollen grains, indehiscent anthers and reduced filament length; the expression of genes controlling stamen development is also altered in rpk2-3 as well as in NPA-treated flower buds. By decreasing auxin biosynthesis or perception in the rpk2-3 background, we eliminated these developmental and gene expression anomalies. • We propose that the auxin maximum in the ML plays a key role in late stamen development, as it ensures correct and coordinated pollen maturation, anther dehiscence and filament elongation. [ABSTRACT FROM AUTHOR]

ABSTRACT The prefrontal cortex has an important role in a variety of cognitive and executive processes, and is generally defined by its reciprocal connections with the mediodorsal thalamic nucleus (MD). The rat MD is mainly subdivided into three segments, the medial (MDm), central (MDc), and lateral (MDl) divisions, on the basis of the cytoarchitecture and chemoarchitecture. The MD segments are known to topographically project to multiple prefrontal areas at the population level: the MDm mainly to the prelimbic, infralimbic, and agranular insular areas; the MDc to the orbital and agranular insular areas; and the MDl to the prelimbic and anterior cingulate areas. However, it is unknown whether individual MD neurons project to single or multiple prefrontal cortical areas. In the present study, we visualized individual MD neurons with Sindbis virus vectors, and reconstructed whole structures of MD neurons. While the main cortical projection targets of MDm, MDc, and MDl neurons were generally consistent with those of previous results, it was found that individual MD neurons sent their axon fibers to multiple prefrontal areas, and displayed various projection patterns in the target areas. Furthermore, the axons of single MD neurons were not homogeneously spread, but were rather distributed to form patchy axon arbors approximately 1 mm in diameter. The multiple-area projections and patchy axon arbors of single MD neurons might be able to coactivate cortical neuron groups in distant prefrontal areas simultaneously. Furthermore, considerable heterogeneity of the projection patterns is likely, to recruit the different sets of cortical neurons, and thus contributes to a variety of prefrontal functions. J. Comp. Neurol. 525:166-185, 2017. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

The aim of this work was to use waste tire rubber (WTR) in the middle layer of hybrid plywood materials. The effects of four variable parameters, namely, WTR contents (430 and 720 g), resin contents (120 and 160 g/m2), hot pressing (single-stage and two-stage), and arrangements of veneer layers on the mechanical, physical, and acoustical properties, were studied. Beech ( Fagus orientalis) and alder ( Alnus glutinosa) veneers having 1.8-mm thickness were used in the production of hybrid plywood panels. Rubber layers of 3- and 5-mm thickness were used in the middle layer of plywood samples. To produce plywood panels, single-stage and two-stage hot-pressing processes were used. Bonding of wood layers was performed using 120 and 160 g/m2 urea-formaldehyde resins, and to form the rubber layers and bond them to wood layers, methylene diisocyanate resin (150 g/m2) was used. Overall trend showed that with the increase in rubber content, the physical properties (water absorption, thickness swelling, and sound absorption) of the manufactured panels were improved, while the mechanical properties (modulus of rapture, modulus of elasticity, and impact strength) of the panels were reduced. Both physical and mechanical performances of plywood panels were improved with increase in resin content. An increase in the WTR content in plywood improved the composite's acoustical property. The production process of the wood/rubber plywood did not significantly affect their properties. The order of improvement in the physical properties of the panels is rubber content > resin content > arrangement of layers > pressing process. Copyright © 2015 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Background and Aims Dioecism characterizes many crop species of economic value, including kiwifruit (Actinidia deliciosa). Kiwifruit male sterility occurs at the microspore stage. The cell walls of the microspores and the pollen of the male-sterile and male-fertile flowers, respectively, differ in glucose and galactose levels. In numerous plants, pollen formation involves normal functioning and degeneration timing of the tapetum, with calcium and carbohydrates provided by the tapetum essential for male fertility. The aim of this study was to determine whether the anther wall controls male fertility in kiwifruit, providing calcium and carbohydrates to the microspores. Methods The events occurring in the anther wall and microspores of male-fertile and male-sterile anthers were investigated by analyses of light microscopy, epifluorescence, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL assay) and transmission electron microscopy coupled with electron spectroscopy. The possibility that male sterility was related to anther tissue malfunctioning with regard to calcium/glucose/galactose provision to the microspores was also investigated by in vitro anther culture. Key Results Both tapetum and the middle layer showed secretory activity and both degenerated by programmed cell death (PCD), but PCD was later in male-sterile than in male-fertile anthers. Calcium accumulated in cell walls of the middle layer and tapetum and in the exine of microspores and pollen, reaching higher levels in anther wall tissues and dead microspores of male-sterile anthers. A specific supply of glucose and calcium induced normal pollen formation in in vitro-cultured anthers of the male-sterile genotype. Conclusions The results show that male sterility in kiwifruit is induced by anther wall tissues through prolonged secretory activity caused by a delay in PCD, in the middle layer in particular. In vitro culture results support the sporophytic control of male fertility in kiwifruit and open the way to applications to overcome dioecism and optimize kiwifruit production. [ABSTRACT FROM AUTHOR]

The signed directed graph (SDG) model can be considered as a qualitative model to describe the variables and their cause–effect relations in a continuous process. Such models can allow one to obtain the fault propagation paths using the method of graph search. In this way, the authors can use SDGs to model and analyse the propagation of faults in large‐scale industrial systems. However, with increasing system scales, the time requirements of a graph search method would be too onerous. This can be alleviated by transforming a single‐layer SDG model into a hierarchical model to improve search efficiency. The hierarchical model would be composed of three layers: the top layer would consist of independent sub‐systems; the middle layer would have control systems configuration and the bottom layer would have all the variables. The possible root causes of faults can then be searched in this model, layer by layer according to the initial response of the system. The efficacy of the proposed approach is illustrated by application to a four‐tank system and a generator system in a power plant. The methodology presented here can also be used in process hazard analysis. [ABSTRACT FROM AUTHOR]

This paper addresses the lack of a cheap reusable software component for building large, database backed, distributed systems. Even after these systems have become mainstream through their use in companies like Google and Amazon, there are still difficult to approach for a small group of programmers with limited resources. We intend to have the JStabilizer framework fill this empty spot and we present in this paper some of the problems we would like to simplify solved and a short description of the solution. [ABSTRACT FROM AUTHOR]

Abstract: The reasons how the middle layer of Ru and Jun porcelain between the glaze and body came into being are still not completely understood. Here, elemental maps from the glaze to the body of pieces of ancient Chinese Ru and Jun porcelain were analyzed by micro-X-ray fluorescence. The results show the middle layer was probably formed by the chemical composition of the glaze turning into glassy states and undergoing complex physical–chemical reactions with the body. However, the middle layer of Jun porcelain was formed by the chemical composition of the glaze turning into glassy states and then infiltrating the body at high temperatures during the firing process. [Copyright &y& Elsevier]

The article focuses on incorporating software robot (Sobot) and mobile robot (Mobot) in middle layer to overcome physical limitations of Sobot for physical behaviors in real situations. The authors propose the basic concept and structure of the middle layer to implement the incorporation of Sobot and Mobot. Real experiments on sensor mapper, behavior mapper, and control arbiter are used to illustrate the effectiveness of the middle layer incorporating Sobot and Mobot. It is stated that the proposed middle layer will play an important role as an interface between Sobot in a virtual environment and Mobot in a real environment.

Autotetraploid rice is a useful rice germplasm for polyploid rice breeding. However, low fertility limits its commercial production. A neo-tetraploid rice with high fertility was developed from the progenies of crossing between autotetraploid lines by our research group. Our previous study showed that a myeloblastosis (MYB) transcription factor, MOF1, might be associated with the pollen development in tetraploid rice. However, little information is available about its role in pollen development in tetraploid rice. Here, we identified a new haplotype of MOF1 from neo-tetraploid rice and marked it as MOF1a. Transcriptome and qRT-PCR analysis demonstrated that MOF1a highly expressed in anthers, and displayed differential expression in neo-tetraploid rice compared to tetraploid rice line with low pollen fertility. The mutant (mof1a) of MOF1a, which was generated by CRISPR/Cas9 knockout in neo-tetraploid rice, showed low pollen fertility, and also exhibited abnormal tapetum and middle layer development, and defective chromosome behaviors during meiosis. A total of 13 tapetal related genes were found to be up-regulated in meiotic anthers of MOF1a compared with wild type plants by RNA-seq analysis, including CYP703A3, PTC1, and OsABCG26, which had been demonstrated to affect tapetal development. Moreover, 335 meiosis-related genes displayed differential expression patterns at same stage, including nine important meiosis-related genes, such as metallothionein OsMT1a. These results demonstrated that MOF1a plays an important role in pollen development and provides a foundation for understanding the molecular mechanism underlying MOF1a in reproduction of tetraploid rice. [ABSTRACT FROM AUTHOR]

Most genetic and molecular analyses of anther development utilize Arabidopsis thaliana, Oryza sativa (rice), and Zea mays (maize). Especially in maize, early stages of anther development are easy to study because: (1) Maize has unisex flowers. (2) Compared to rice or A. thaliana, maize anthers are relatively large, making dissection for molecular and biochemical analyses easy. (3) Anther developmental stage is strongly correlated with maize anther length. Besides these technical advantages, understanding anther and pollen development in maize is of significant agricultural importance. Today maize is a worldwide cereal crop: approximately 25% of all consumed food contains maize. Yield stability or even increases depend on maintenance of hybrid vigor, and production of hybrid seed requires manual detasseling or genetic control of pollen development. Knowledge of pollen development can also be used to manage transgene containment. In the first section of this chapter, we will describe the current model for sequential cell fate specification in maize anther lobes, with reference to rice and A. thaliana to point out similarities and differences. In the second section of this chapter, we will review what is known about the individual cell types in anther lobes. The diversity of anther organization is addressed to a limited extent by cytological studies of anthers, often directed to clarify taxonomic relationships. In the third section, we will comment on how new lines of investigation could clarify questions remaining in our current appreciation of anther development., (© 2019 Elsevier Inc. All rights reserved.)

The Piwi genes have an important role in stem cell development, gametogenesis and RNA interference in diverse organisms. So far, most of the studies have focused on the function of Piwis in vertebrates, but their function during spermiogenesis in invertebrates still remains largely unclear. In order to investigate the function of Piwis during spermiogenesis in the crab Portunus trituberculatus, we use RT-PCR and RACE to identify three Piwi complete cDNA sequences from the total RNA of the testis in P. trituberculatus. The deduced amino acid sequences of P. trituberculatus Piwi-1, Piwi-2 and Piwi-3 showed that each contains a well-conserved PAZ domain and PIWI domain. RT-PCR analyzed the tissue expression pattern of P. trituberculatus Piwi-1, Piwi-2 and Piwi-3 in the testis, heart, muscle, hepatopancreas and gill. All of the Piwis are found in germ cells of adult testis in P. trituberculatus by in situ hybridization, suggesting that these genes may play function during spermiogenesis in this species., (Copyright © 2013 Elsevier B.V. All rights reserved.)