24 results on '"Miao, XinYue"'
Search Results
2. Study on the mechanism of Rb1 regulating antioxidant defense system and endogenous hormones leading to increased virulence of Panax ginseng
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Yan, Ning, Miao, Xinyue, Zhan, Yu, Wang, Ergang, Chen, Changbao, and Li, Qiong
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- 2024
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3. Synchronous defect passivation strategy via Lewis base for efficient and stable perovskite solar cells
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Liu, Hongtao, Miao, Xinyue, Wang, Kelin, Gao, Jieyu, Geng, Hailong, and Deng, Xiong
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- 2022
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4. Facilitating Effects of Reductive Soil Disinfestation on Soil Health and Physiological Properties of Panax ginseng
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Zhan, Yu, Wang, Ergang, Zhou, Yi, He, Guixiang, Lv, Pengyuan, Wang, Lixiang, Zhou, Tingting, Miao, Xinyue, Chen, Changbao, and Li, Qiong
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- 2024
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5. Layer-controlled fractal growth of vanadium-doped molybdenum disulfide.
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Miao, Xinyue, Xu, Yingjie, Chen, Hongyu, Ma, Xiying, Wang, Fei, Zhang, Xiaohua, and Zou, Jingyun
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BIOELECTRONICS ,TRANSITION metals ,VANADIUM ,DOPING agents (Chemistry) ,MOLYBDENUM disulfide ,MOLYBDENUM sulfides ,ETCHING - Abstract
Vanadium doping can effectively modify the physical properties of transition metal dichalcogenides (TMDCs), broadening their application prospect in electronic, spintronic, and valleytronic devices. However, vanadium dopants always suppress the growth of TMDCs and lower the growth controllability. Here, we report the morphology-controlled growth of vanadium-doped MoS
2 (V-MoS2 ). Both the layer-controlled growth and fractal growth of monolayer V-MoS2 were realized, and the mechanism was analyzed, including contributions from the localized concentration of Mo atoms, the etching effect, and formation energies of different terminations. It will shed light on the morphology-controlled growth of other vanadium-doped TMDCs, promoting the construction of novel devices. Controll vanadium dopants enables layer-controlled and fractal growth of V-MoS2 in this work with contributions from the localized concentration of Mo atoms, the etching effect, and formation energies of different terminations. It will help to realize the morphology-controlled growth of other vanadium-doped TMDCs and thus promotes the construction of novel devices. [ABSTRACT FROM AUTHOR]- Published
- 2024
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6. Insight of the Chinese enterprises' investment performance along 'one belt one road'.
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Xue, Rui, Liu, HuiZheng, Baron, Claude, Miao, XinYue, and Jafri, Muhammad Afaq Haider
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BELT & Road Initiative ,FOREIGN investments - Abstract
More and more Chinese enterprises have participated in the Belt and Road initiative (BRI) since its launch in 2013. However, have they all actually benefited? Which companies otherwise have benefited most from the plan? To answer these questions, this paper analyzes the impact of the BRI on Chinese enterprises. Among several findings, it reveals that the performance of companies remained low even though the initiative policy initially stimulated performance, the performance of enterprises depends on the geographical location of enterprises and that the returns of China's outward foreign direct investment in various industries became more volatile with only the mining industry enjoying better returns. Thus, the paper shows that the BRI offers Chinese enterprises opportunities but also brings challenges for companies to optimize the allocation of their resources and improve the performance of their investments. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Fe Complex-Based Catalyst System for the Chirality-Selected Continuous Synthesis of Single-Walled Carbon Nanotubes.
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Xu, Yingjie, Miao, Xinyue, Chen, Hongyu, Gu, Xu, Liu, Yi, Ma, Chunlan, Zhang, Xiaohua, and Zou, Jingyun
- Abstract
After decades of development, the single-walled carbon nanotube (SWCNT) has found its advantage in the application of logic devices and energy applications. However, the difficulty in the chirality-selected growth and mass production of the SWCNT impedes its commercial application, especially in field effect transistors (FETs) and solid-state electrodes used in lithium-ion batteries. The injection chemical vapor deposition (iCVD) is one of the most promising methods to realize the mass production of the SWCNT, during which the catalyst plays a key role in determining the diameter, wall number, and even chirality of as-grown carbon nanotubes. Here, an Fe complex-based catalyst system is designed to realize the chirality-selected continuous growth of the SWCNT. The introduction of NaCl can reduce the size of the formed localized liquid zones and enrich S atoms in these liquid zones, which contributes to the growth of the SWCNT. The different combinations of Fe-(Ddtc)
2 (Phen) (Phen = 1,10-phenanthroline and Ddtc = diethyldithiocarbamate) and ferrocene then promote the growth of the SWCNT with different chiralities. The continuous growth progress confirms the high efficiency of this catalyst system, and the Raman and transmission electron microscopy (TEM) characterizations validate its effectiveness in the chirality-selected synthesis of the SWCNT. This study will provide an idea for the fine mass production of the SWCNT. [ABSTRACT FROM AUTHOR]- Published
- 2024
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8. A green, rapid, scalable and versatile hydrothermal strategy to fabricate monodisperse carbon spheres with tunable micrometer size and hierarchical porosity
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Zhao, Qiming, Tao, Shan, Miao, Xinyue, and Zhu, Yan
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- 2019
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9. An efficient plug-and-play regularization method for full waveform inversion.
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Fu, Hongsun, Yang, Lu, and Miao, Xinyue
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CONVOLUTIONAL neural networks ,ARTIFICIAL neural networks ,NONLINEAR equations - Abstract
Nonlinear inverse problems arise in various fields ranging from scientific computation to engineering technology. Inverse problems are intrinsically ill-posed, and effective regularization techniques are necessary. The core of a suitable regularization method is to introduce the prior information of the model via an explicit or implicit regularization function. Plug-and-play regularization is a flexible framework that integrates the most effective denoising priors into an iterative algorithm, and it has recently shown great potential in the solution of linear ill-posed problems. Unlike traditional regularization methods, plug-and-play regularization does not require an explicit regularization function to represent the prior information of the model. In this work, by using total variation, block-matching and three-dimensional filtering, and fast and flexible denoising convolutional neural network denoisers, we propose a novel iterative regularization algorithm based on the alternating direction method of multipliers method. The combination of total variation and block-matching three-dimensional filtering regularizers can take advantage of the sparsity and nonlocal similarity in the solution of inverse problems. When combined with traditional and novel regularizers, deep neural networks have been shown to be an effective regularization approach, which can achieve state-of-the-art performance. Finally, we apply the proposed algorithm to the full waveform inversion problem to show the effectiveness of our method. Numerical results demonstrate that the proposed algorithm outperforms existing inversion methods in terms of quantitative measures and visual perceptual quality. [ABSTRACT FROM AUTHOR]
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- 2023
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10. How to promote the sustainable development of virtual reality technology for training in construction filed: A tripartite evolutionary game analysis.
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Shi, Chunyan, Miao, Xinyue, Liu, Hui, Han, Yang, Wang, Yafei, Gao, Weijun, Liu, Gen, Li, Siwen, Lin, Yingzi, Wei, Xindong, and Xu, Tongyu
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VIRTUAL reality , *SUSTAINABLE development , *PRODUCT life cycle , *GOVERNMENT policy , *QUANTITATIVE research , *EVOLUTIONARY algorithms - Abstract
In recent years, virtual reality training technology (VRTT) has been considered by many scholars as a new training method instead of traditional training (TT) to reduce unsafe behaviors ascribed to construction workers (CWs) and corporate accident rates. However, in this process, a conflict of interest arises among the government, construction enterprises (CEs), and CWs. Therefore, this study introduces a quantitative research method, the three-party evolutionary game and creatively combining them with the product life cycle (PLC) to solve this problem by analyzing the equilibrium and evolutionarily stable strategies of the system. Finally, collaborative players' decision-making behaviors and their sensitivity to critical factors are examined. This paper will illustrate these in each stage through numerical simulations. The results of the study indicate that the government plays a dominant role in the VRTT introduction stage. When the government gives CEs appropriate subsidies, CEs will eventually realize the importance of VRTT for CWs. Then the government will gradually reduce the amount of the subsidies in this process. In addition, we also find that the continually high cost will lead to negative policies by the government, which requires the active cooperation and attitude change from CEs and CWs. Ultimately, the government, CEs and CWs adopt the best strategy in the evolutionary process to facilitate the promotion, application and sustainability of VRTT in the construction industry. [ABSTRACT FROM AUTHOR]
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- 2023
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11. Covalent hyperbranched porous carbon nanospheres as a polymeric stationary phase for ion chromatography
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Zhao, Qiming, Miao, Xinyue, Yu, Jianan, and Zhu, Yan
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- 2019
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12. Genome-Wide Analysis and Expression Profiling of the Glutathione Peroxidase-like Enzyme Gene Family in Solanum tuberosum.
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Wang, Shenglan, Sun, Xinxin, Miao, Xinyue, Mo, Fangyu, Liu, Tong, and Chen, Yue
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GLUTATHIONE peroxidase ,GENE expression ,GENE families ,GLUTATHIONE ,POTATOES ,FOOD crops ,REACTIVE oxygen species - Abstract
Glutathione peroxidase-like enzyme is an important enzymatic antioxidant in plants. It is involved in scavenging reactive oxygen species, which can effectively prevent oxidative damage and improve resistance. GPXL has been studied in many plants but has not been reported in potatoes, the world's fourth-largest food crop. This study identified eight StGPXL genes in potatoes for the first time through genome-wide bioinformatics analysis and further studied the expression patterns of these genes using qRT-PCR. The results showed that the expression of StGPXL1 was significantly upregulated under high-temperature stress, indicating its involvement in potato defense against high-temperature stress, while the expression levels of StGPXL4 and StGPXL5 were significantly downregulated. The expression of StGPXL1, StGPXL2, StGPXL3, and StGPXL6 was significantly upregulated under drought stress, indicating their involvement in potato defense against drought stress. After MeJA hormone treatment, the expression level of StGPXL6 was significantly upregulated, indicating its involvement in the chemical defense mechanism of potatoes. The expression of all StGPXL genes is inhibited under biotic stress, which indicates that GPXL is a multifunctional gene family, which may endow plants with resistance to various stresses. This study will help deepen the understanding of the function of the potato GPXL gene family, provide comprehensive information for the further analysis of the molecular function of the potato GPXL gene family as well as a theoretical basis for potato molecular breeding. [ABSTRACT FROM AUTHOR]
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- 2023
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13. Promoting Sponge City Construction through Rainwater Trading: An Evolutionary Game Theory-Based Analysis.
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Shi, Chunyan, Miao, Xinyue, Xu, Tongyu, Gao, Weijun, Liu, Gen, Li, Siwen, Lin, Yingzi, Wei, Xindong, and Liu, Hui
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RAINWATER ,WEATHER & climate change ,EXTREME weather ,CLIMATE change ,LABOR incentives - Abstract
Sponge city construction strategies (SCCSs) have gradually attracted increased attention because of the strong shocks to society and economies caused by extreme weather and global climate change. The development of sponge cities is consistent with the national goal, and China must support environmental sustainability. Rainwater trading (RWT) plays a key role in promoting the efficient allocation and use of rainwater resources in sponge cities. In this study, we built an evolutionary game model on the basis of 13 parameters influencing the strategy selection of game players of environmental protection enterprises (EPEs) and municipal enterprises (MEs)' in promoting sponge city construction. Next, we discussed the interaction effect of the two players' behaviors in the 16 cases. Finally, we used the first RWT project in an empirical simulation to analyze the critical parameters influencing the game; we provide regulation policy suggestions to achieve the final goal. The results show that sufficient financial subsidies, the reduction in additional sales, the increase in taxes, and the participation of more EPEs can accelerate the realization of the evolutionary stable strategy (ESS) between EPEs and MEs. Incentive measures should focus not only on economic measures but also on reputation incentives and industry regulations. The proposed model can be used as a tool to promote the development and application of sponge cities, thus enriching the literature on promoting the communication of SCCSs. Moreover, our findings are valuable for the promotion of the use of rainwater resources, the marketization of the ecological value of rainwater resources, and the further construction of sponge cities. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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14. The potential of novel bacterial isolates from healthy ginseng for the control of ginseng root rot disease (Fusarium oxysporum).
- Author
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Li, Qiong, Yan, Ning, Miao, Xinyue, Zhan, Yu, and Chen, Changbao
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ROOT rots ,GINSENG ,FUSARIUM oxysporum ,FUNGAL cell walls ,INDOLEACETIC acid ,PATHOGENIC fungi - Abstract
Ginseng root rot caused by Fusarium oxysporum is serious disease that impacts ginseng production. In the present study, 145 strains of bacteria were isolated from the rhizosphere soil of healthy ginseng plants. Three strains with inhibitory activity against Fusarium oxysporum (accession number AF077393) were identified using the dual culture tests and designated as YN-42(L), YN-43(L), and YN-59(L). Morphological, physiological, biochemical, 16S rRNA gene sequencing and phylogenetic analyses were used to identify the strains as Bacillus subtilis [YN-42(L)] (accession number ON545980), Delftia acidovorans [YN-43(L)] (accession number ON545981), and Bacillus polymyxae [YN-59(L)] (accession number ON545982). All three isolates effectively inhibited the growth of Fusarium oxysporum in vitro and the antagonistic mechanism used by the three strains involved the secretion of multiple bioactive metabolites responsible for the hydrolysis of the fungal cell wall. All three biocontrol bacteria produce indoleacetic acid, which has a beneficial effect on plant growth. From our findings, all three antagonistic strains can be excellent candidates for ginseng root rot caused by the pathogenic fungus Fusarium oxysporum. These bacteria have laid the foundation for the biological control of ginseng root rot and for further research on the field control of ginseng pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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15. Can Professionalization Alleviate Job Burnout in Construction Workers in China? A Multivariable Mediating Model.
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Ni, Guodong, Miao, Xinyue, Li, Li, Li, Huaikun, Wang, Shaobo, and Niu, Miaomiao
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- 2022
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16. A Real-Time Fatigue Sensing and Enhanced Feedback System.
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Miao, Xinyue, Xue, Chengqi, Li, Xian, and Yang, Lichun
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PSYCHOLOGICAL feedback , *EYE movements , *SYSTEMS design , *OPERATIONAL risk , *SENSES - Abstract
This paper describes a real-time fatigue sensing and enhanced feedback system designed for video terminal operating groups. This paper analyzes the advantages and disadvantages of various current acquisition devices and various algorithms for fatigue perception. After comparison, this study uses an eye movement instrument to collect user PERCLOS, and then calculates and determines the user's fatigue state. A detailed fatigue discrimination calculation method is provided in this paper. The fatigue level is divided into three levels: mild fatigue, moderate fatigue and severe fatigue. Finally, this study uses the fatigue method demonstrated above to achieve real-time discrimination of the fatigue level of the user in front of the video operation terminal. This paper elaborates a method for waking up users and enhancing feedback based on their fatigue level and the importance of information. This study provides a solution for avoiding the operational risks caused by fatigue and lays the foundation for the machine to sense the user and provide different service solutions based on the user's status. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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17. Formation Mechanism and Dynamic Evolution Laws About Unsafe Behavior of New Generation of Construction Workers Based on China's Construction Industry: Application of Grounded Theory and System Dynamics.
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Ni, Guodong, Lv, Lei, Wang, Shaobo, Miao, Xinyue, Fang, Yaqi, and Liu, Qing
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CONSTRUCTION workers ,GROUNDED theory ,SYSTEM dynamics ,SYSTEMS theory ,CONSTRUCTION industry - Abstract
Construction workers' unsafe behavior is a major cause of safety accidents and injuries, therefore, a profound understanding of the formation process and evolution laws about construction workers' unsafe behavior is conducive to taking measures to prevent incidents. At present, the new generation of construction workers (NGCWs) born after 1980 are gradually becoming the main force at construction sites in China. Given that generational differences of construction workers can cause the discrepancies in their thoughts and attitudes when engaging in safety-related activities, this study aims to investigate the formation mechanism and dynamic evolution laws about NGCWs' unsafe behavior based on the context of China's construction industry. From the perspective of behavior motivation, in-depth semi-structured interviews with 18 NGCWs and 7 grassroots managers were conducted, and data analysis followed a three-step coding process based on grounded theory. Through continuous comparison, abstraction and analysis, the stimulus-organism-response theory was introduced and expanded to construct a three-stage formation mechanism model. On this basis, the causal diagram and stock flow diagram were developed based on system dynamics principles to reflect the dynamic feedback relationships of the factors in the static formation mechanism model, and simulation was carried out using Vensim PLE software. The results show that three types of internal needs and three types of external incentives stimulate corresponding motivations for NGCWs' unsafe behavior. Two types of individual factors, five types of situational factors and behavior result play an influencing role in the decision-making process of externalizing motivation into behavior. Under the synergistic effect of multiple factors, the level of unsafe behavior displays a downward trend, and the rate of decrease is slow first and then fast. Furthermore, among individual factors and situational factors, safety awareness and safety management system have the most significant effect on the level of unsafe behavior, while situational factors play a more obvious role. The findings can provide theoretical support and practical references to China's construction companies and government departments for the purpose of improving NGCWs' unsafe behavior. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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18. Different Responses of Soil Environmental Factors, Soil Bacterial Community, and Root Performance to Reductive Soil Disinfestation and Soil Fumigant Chloropicrin.
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Zhan, Yu, Yan, Ning, Miao, Xinyue, Li, Qiong, and Chen, Changbao
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CHLOROPICRIN ,FUMIGANTS ,NUCLEOTIDE sequencing ,ACID phosphatase ,SOILS ,BACTERIAL population ,BACTERIAL communities - Abstract
Reductive soil disinfestation (RSD) and soil fumigant chloropicrin (SFC) are two common agricultural strategies for the elimination of soil-borne pathogens. However, the differences in soil environmental factors, soil bacterial microbiome, and root performance between SFC and RSD are poorly understood. In this study, three soil treatments, untreated control (CK), SFC with 0.5 t⋅ha
–1 chloropicrin, and RSD with 15 t⋅ha–1 animal feces, were compared. We evaluated their effects on soil environmental factors, bacterial community structure, and root activity using chemical analysis and high-throughput sequencing. RSD treatment improved soil composition structure, bacterial diversity, and root performance to a greater extent. Carbon source utilization preference and bacterial community structure were strikingly altered by SFC and RSD practices. Bacterial richness, diversity, and evenness were notably lowered in the SFC- and RSD-treated soil compared with the CK-treated soil. However, RSD-treated soil harbored distinct unique and core microbiomes that were composed of more abundant and diverse potentially disease-suppressive and organic-decomposable agents. Also, soil bacterial diversity and composition were closely related to soil physicochemical properties and enzyme activity, of which pH, available Na (ANa), available Mg (AMg), available Mn (AMn), total Na (TNa), total Ca (TCa), total Cu (TCu), total Sr (TSr), urease (S-UE), acid phosphatase (S-ACP), and sucrase (S-SC) were the main drivers. Moreover, RSD treatment also significantly increased ginseng root activity. Collectively, these results suggest that RSD practices could considerably restore soil nutrient structure and bacterial diversity and improve root performance, which can be applied as a potential agricultural practice for the development of disease-suppressive soil. [ABSTRACT FROM AUTHOR]- Published
- 2021
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19. INVESTIGATION OF THE DYNAMIC CHARACTERISTICS OF A THERMAL ENERGY STORAGE UNIT FILLED WITH MULTIPLE PHASE CHANGE MATERIALS.
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LI, Xiaoyan, HUANG, Rongpeng, MIAO, Xinyue, WANG, Xuelei, LIU, Yabin, and ZHANG, Xiaoya
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HEAT storage devices ,PHASE change materials ,AIR conditioning ,LATENT heat ,MATHEMATICAL models ,HEAT transfer - Abstract
In order to improve the thermal performance of thermal energy storage systems, a packed bed thermal energy storage systems unit using spherical capsules filled with multiple phase change materials (multi-PCM) for use in conventional air-conditioning systems is presented. A 3-D mathematical model was established to investigate the charging characteristics of the thermal energy storage systems unit. The optimum proportion between the multi-PCM was identified. The effects of heat transfer fluid-flow rate and heat transfer fluid inlet temperature on the liquid phase change materials volume fraction, charging time and charging capacity of the thermal energy storage system unit are studied. The results indicate that the charging capacity of multi-PCM units is higher than that of the conventional single-PCM (HY-2). For proportions 0:1:0, 2:3:3, 3:2:3, 3:3:2, 4:1:3, and 4:2:2, the charging capacity decreases by approximately 24.84%, 14.69%, 6.47%, 3.82%, and 1.13%, respectively, compared to the 4:2:2 proportion. Moreover, decreasing the heat transfer fluid inlet temperature can obviously shorten the complete charging time of the thermal energy storage systems unit. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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20. Improving the professionalization level of a new generation of construction workers to reduce their unsafe behaviour: an empirical study in China.
- Author
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Ni G, Wang S, Zhou Q, Qiao Y, Ojum C, Li H, and Miao X
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- Humans, China, Cross-Sectional Studies, Adult, Male, Female, Surveys and Questionnaires, Middle Aged, Occupational Health, Construction Industry, Burnout, Professional psychology, Burnout, Professional prevention & control
- Abstract
Objectives . Improvement of the professionalization level for the new generation of construction workers (NGCWs) is critical to upgrade the construction industry. It also provides a new approach to reduce their unsafe behaviour. The purpose of this study is to analyse the correction mechanism of the professionalization level on NGCWs' unsafe behaviour. We examined the mediating role of work-family conflict and job burnout on impacting NGCWs' unsafe behaviour. The moderation effect of the NGCWs' perceived organizational support was also examined. Methods. The cross-sectional study was conducted based on structural equation modelling, confirmatory factor analysis and regression analysis with a sample of 496 NGCWs in China. Results. The professionalization level can effectively correct NGCWs' unsafe behaviour. Job burnout can independently mediate the relationship between professionalization level and unsafe behaviour and act as a serial mediator for work-family conflict. Moreover, the high level of perceived organizational support will effectively suppress the effect of work-family conflict on job burnout. Conclusions . Improving the professionalization level is an effective way to correct NGCWs' unsafe behaviour. Moreover, management should help the NGCWs balance work and family, to alleviate burnout. Additionally, it is suggested that management should ensure the NGCWs perceive the organizational support.
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- 2024
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21. Effects of reductive soil disinfestation combined with different types of organic materials on the microbial community and functions.
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Zhan Y, Zhou Y, Wang E, Miao X, Zhou T, Yan N, Chen C, and Li Q
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- Agriculture methods, Bacteria, Nitrogen, Soil Microbiology, Soil chemistry, Microbiota
- Abstract
Reductive soil disinfestation (RSD) is an effective method to inhibit soilborne pathogens. However, it remains unclear how RSD combined with different types of organic materials affects the soil ecosystems of perennial plants. Pot experiments were conducted to investigate the effects of RSD incorporated with perilla (PF), alfalfa (MS), ethanol, and acetic acid on soil properties, enzyme activities, microbial communities and functions, and seedling growth. Results showed that RSD-related treatments improved soil properties and enzyme activities, changed microbial community composition and structure, enhanced microbial interactions and functions, and facilitated seedling growth. Compared with CK, RSD-related treatments increased soil pH, available nitrogen, and available potassium contents, sucrase and catalase activities, and decreased soil electric conductivity values. Meanwhile, RSD-related treatment also significantly reduced the relative abundance of Fusarium while increasing the relative abundance of Arthrobacter , Terrabacter , and Gemmatimonas . The reduction was more evident in PF and MS treatment, suggesting the potential for RSD combined with solid agricultural wastes to suppress pathogens. Furthermore, the microbial network of RSD-related treatment was more complex and interconnected, and the functions related to carbon, nitrogen, sulfur, and hydrogen cycling were significantly increased, while the functions of bacterial and fungal plant pathogens were decreased. Importantly, RSD-related treatments also significantly promoted seed germination and seedling growth. In summary, RSD combined with solid agricultural wastes is better than liquid easily degradable compounds by regulating the composition and function of microbial communities to improve soil quality and promote plant growth.IMPORTANCEReductive soil disinfestation (RSD) is an effective agricultural practice. We found that RSD combined with solid agricultural wastes is better than that of liquid easily degradable compounds, may improve soil quality and microbial community structure, inhibit the proliferation of pathogenic bacteria, and contribute to the growth of replanted crops. Thus, RSD combined with solid agricultural wastes is more effective than liquid easily degradable compounds., Competing Interests: The authors declare no conflict of interest.
- Published
- 2024
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22. First Report of Pantoea agglomerans Causing Leaf Blight on Schisandra chinensis in China.
- Author
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Yan N, Miao X, Zhan Y, Zhou Y, Wang E, Chen C, and Li Q
- Abstract
Schisandra chinensis (Turcz.) Baill is a perennial liana, which is widely cultivated and used in China. In August 2022, Schisandra chinensis leaves with small light brown spots were found on plants growing in Fusong (127°28'E, 42°33'N) of China. There was 15% disease incidence and 50% disease severity of Schisandra chinensis in 2-ha fields of S. chinensis. As the disease progressed, the spots become darker and form round or irregular concentric circles. Leaves with brown spot symptoms were collected from the field. Leaf pieces (5 mm × 5 mm) were excised from lesion margins, surface disinfected with 75% ethanol for 1 min, followed by 1.5% sodium hypochlorite for 3 min, and incubated on Luria Bertani (LB) solid medium at 28°C for 24 hours. Eight cultures were isolated, and representative single colony (XWWZH) was selected from the pure cultures according to colony characteristics for observation The purified colonies were round, yellow, and slimy, cells were straight rod-shaped (0.40 to 0.52 × 1.12 to 1.69 µm) were observed. The isolate was Gram negative. It was positive for methyl red reaction, lysine decarboxylase reaction, gelatin hydrolysis reactionand sucrose utilization. It was negative for indole reaction and produced H2S. The bacterium was preliminarily identified as Pantoea agglomerans based on morphological and biochemical tests (Baird et al. 2007). The 16S rDNA and a portion of rpoB of strain XWWZH were amplified and sequenced. The sequences were submitted to GenBank. (Accession OP763753 and OQ813505, respectively). Phylogenetic trees were constructed based on the 16S rDNA and rpoB gene sequences. The sequences of strain XWWZH clustered with strains P. agglomerans deposited in GenBank. The pathogenicity was verified with non-wounded S. chinensis seedlings by punching holes with sterile needles and injecting a solution of 1 × 108 CFU/ml solution. Sterile ddH2O was injected in the control experiment. The inoculated seedlings were incubated in a greenhouse at 25°C with a relative humidity of 65 to 70%. Five to eight days after inoculation, inoculated leaves, exhibited symptoms which were morphologically identical to those of the originally infected leaves whereas control plants remained asymptomatic. The pathogenicity assays were repeated twice with the same results. The re-isolated pathogen had the same morphology and DNA sequences as the original isolate obtained from the field samples, completing Koch's postulates. Strains of P. agglomerans have been reported to severely infect many plants (Ren et al.2008; Lee et al. 2010; Yang et al. 2011; Guo et al. 2019; Gao et al, 2022), but to the best of our knowledge, this is the first report of a strain of P. agglomerans causing leaf blight on Schisandra chinensis in China. The identification of leaf blight caused by P. agglomerans will enable farmers to prevent and manage it ahead of time to reduce losses.
- Published
- 2023
- Full Text
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23. First Report of Fusarium acuminatum Causing Leaf Spot on Schisandra chinensis in China.
- Author
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Zhan Y, Yan N, Miao X, Wang E, Zhou Y, Chen C, and Qiong L
- Abstract
Schisandra chinensis (Turcz.) Baill. is a popular and widely cultivated medicinal herb in China, which has rich nutritional value and medicinal effect. In August 2022, leaves with oval and irregularly circular light brown spots from 2 to 10 mm wide with white centers were found on Schisandra chinensis growing in Fusong district (127°28'E, 42°33'N) of Jilin, China. The symptoms were observed in 20% of the plants of a 2 ha-1 field of Schisandra chinensis. About 50% of the leaf areas were affected. As the disease developed, the lesions grew larger and developed necrotic centers. Leaves with light brown spot symptoms from five plants were collected from the field. Five leaf pieces (3 to 5 mm2) were excised from lesion margins, surface sterilized based on Ju et al. (Ju et al. 2021), and incubated on potato dextrose agar (PDA) at 25°C. Six single spores were isolated from five independently infected isolates for pure culture using the single spore isolation technique (Zhang. 2003). Representative single spore isolate (ZWWZH) was selected from pure cultures for further culture. After 5 days, fluffy white aerial mycelium with pink pigmentation on the underside of the colony were observed on PDA. Mycelia became pinkish-brown as the culture aged. Microscopic observations showed the presence of elongated or pointed, and thick-walled macroconidia (n = 50), predominantly three septate, 3.40 to 7.50 × 40.34 to 61.29 μm were observed. Chlamydospores formed in chains within or on top of the mycelium. The primers ITS1/ITS4 (White et al. 1990) and Bt-2a/Bt-2b (Robideau et al., 2011) were used to amplify the internal transcribed spacer (ITS) rDNA and β-tubulin (TUB2) region, respectively. The obtained sequences were submitted to GenBank under accession numbers for OQ629789 (ITS) and OQ803521(TUB2). BLASTn analysis of both ITS sequence and TUB2 sequence, revealed 100% and 99.92% sequence identity with F. acuminatum MT566456, MT560377 and KJ396328, respectively. The pathogen was identified as F. acuminatum based on morphological and molecular data. Pathogenicity tests were carried out in the greenhouse. Select five healthy Schisandra chinensis seedlings, each with each healthy leaf surfaces inoculated a 1 × 106 spores/mL solution, 3 wells on one side, 10 µL per well. Sterile ddH2O was used in the control experiment. The inoculated seedlings were incubated at 25°C with a relative humidity of 65 to 70% in a greenhouse. Four days after inoculation, all inoculated leaves exhibited the same symptoms as observed in the field, while the controls showed no symptoms. The experiment was repeated three more times with similar results. The re-isolated fungi from the inoculated plants had the same morphology and DNA sequences as the original isolate (ZWWZH) obtained from the field samples, completing Koch's postulates. To our knowledge, this is the first report of F. acuminatum causing leaf spot on Schisandra chinensis in China. F. acuminatum has seriously affected the quality of Schisandra chinensis production. The identification of leaf spot caused by F. acuminatum will enable farmers to identify practices to minimize disease on this important crop.
- Published
- 2023
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24. Effect of ginsenosides on microbial community and enzyme activity in continuous cropping soil of ginseng.
- Author
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Miao X, Wang E, Zhou Y, Zhan Y, Yan N, Chen C, and Li Q
- Abstract
Root exudates contain plant metabolites secreted by the roots into the soil, such as ginsenosides secreted by the ginseng root. However, little is known about ginseng root exudate and its impact on the chemical and microbial properties of soil. In this study, the effect of increasing concentrations of ginsenosides on the chemical and microbial properties of soil was tested. Chemical analysis and high-throughput sequencing techniques were used to evaluate the soil chemical properties and microbial characteristics following exogenous application of 0.1 mg·L
-1 , 1 mg·L-1 , and 10 mg·L-1 ginsenosides. Ginsenosides application significantly altered soil enzyme activities; SOM-dominated physicochemical properties were significantly reduced which altered the composition and structure of the soil microbial community. In particular, treatment with 10 mg∙L-1 ginsenosides significantly increased the relative abundance of pathogenic fungi such as Fusarium, Gibberella and Neocosmospora . These findings indicate that ginsenosides in root exudates are important factors that may lead to increased deterioration of soil during ginseng cultivation and provided new research direction for the subsequent study on the mechanism of interaction between ginsenosides and soil microbial communities., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Miao, Wang, Zhou, Zhan, Yan, Chen and Li.)- Published
- 2023
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