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This study aimed to examine the potential of soil amendment-assisted phytoremediation using ryegrass in reclaiming abandoned gold mine soil in southwestern Ghana, with a specific focus on the soil contamination hazards associated with metals and metalloids. A pot experiment lasting 60 days was carried out to assess the efficacy of soil amendments, such as compost, iron oxide, and poultry manure, in mitigating environmental hazards. Three soil contamination indices (soil contamination = CF, enrichment factor = ER, and pollution load index = PLI) were used to calculate the extent of soil contamination, enrichment, and pollution of the sites with Co, Hg, Ni, Mo, Se, Sb, and Pb. The findings show that Hg made the greatest contribution (with a maximum soil CF of 18.0) to the overall PLI, with a maximum value of 74.4. The sites were averagely and consequently enriched with toxic elements in the decreasing order: Ni (ER = 33.3) > Mo (20.5) > Sb (14.1) > Pb (11.0) > Hg (7.9) > Se (2.1). The bioaccumulation factor (BCF > 1) suggests that ryegrass has the ability to phytostabilize Co, Hg, Mo, and Ni. This means that the plant may store these elements in its roots, potentially decreasing their negative effects on the environment and human health. Ultimately, the addition of combined manure with iron oxides might have augmented the sequestration of these metals in the root. The elements may have accumulated through sorption on manure or Fe surfaces, dissolution from watering the plants in the pot, or mineralization of organic manure. Thus, ryegrass has shown potential for phytostabilisation of Co, Hg, Mo, and Ni when assisted with a combination of manure and iron oxides; and can consequently mitigate the environmental and human health impacts.

This comprehensive guideline, developed by a representative group of UK-based medical experts specialising in haemoglobinopathies, addresses the management of conception and pregnancy in patients with thalassaemia. A systematic search of PubMed and EMBASE using specific keywords, formed the basis of the literature review. Key terms included "thalassaemia," "pregnancy," "Cooley's anaemia," "Mediterranean anaemia," and others, covering aspects such as fertility, iron burden and ultrasonography. The guideline underwent rigorous review by prominent organisations, including the Endocrine Society, the Royal College of Obstetricians and Gynaecologists (RCOG), the United Kingdom Thalassaemia Society and the British Society of Haematology (BSH) guideline writing group. Additional feedback was solicited from a sounding board of UK haematologists, ensuring a thorough and collaborative approach. The objective of the guideline is to equip healthcare professionals with precise recommendations for managing conception and pregnancy in patients with thalassaemia., (© 2024 British Society for Haematology and John Wiley & Sons Ltd.)

Abandoned gold mining spoils pose socio-environmental, human, and animal health impacts and threaten sustainability of mineral extraction. Green trials and ecological solutions are required to effectively remediate these contaminated soils and mitigate the associated risks. Here, we carried out a pot experiment using a highly contaminated soil (mean total As = 5104.0 mg/kg) collected from an abandoned mine spoil in Ghana. We aimed to quantify the impacts of compost, iron oxide, and poultry manure on the mobilization, fractionation, and uptake of As by ryegrass (Lolium perenne). The soil amendments were applied at a rate of 5% (w/w) each, separately or in combination. We extracted the mine spoil soil readily-bioavailable As and specific-sorbed As, and determined the As contents in plant and the uptake after harvest. The plant transfer indices for soil-to-root (bioconcentration factor, BCF), soil-to-shoot (bioaccumulation concentration- BAC), and root-to-shoot (translocation factor- TF) were also calculated. Addition of manure increased the mining readily-bioavailable As by 243% and specific-sorbed As by 38%, as compared to the control. Manure addition further aided root As-uptake by 134%, whilst its combination with compost increased uptake by 101%. Lone addition of manure and in combination with compost resulted in BCF above 1, indicating increased As-phytostability. The presence of carbon and iron in the roots of the ryegrass sorbed or precipitated As limited its soil-to-shoot and root-to-shoot transfer. These findings indicate that manure alone and in combination with compost can be used to augment the phytoremediation efficiency of ryegrass in the As-contaminated spoil., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Arsenic (As) redox-induced mobilisation and speciation in polluted gold mine sites in tropical climates largely remains unknown. Here, we investigated the impact of changes in soil redox potential (E H ) (-54 mV to +429 mV) on mobilisation of As and its dominant species in an abandoned spoil (total As = 4283 mg/kg) using an automated biogeochemical microcosm set-up. Arsenic mobilisation increased (85-137 mg/L) at moderately reducing conditions (-54 mV to + 200 mV)), while its reduced (6-35 mg/L) under oxic conditions (+200 to +400 mV). This indicates the high risk of As potential loss under reducing conditions. The mobilisation of As was governed by the redox chemistry of Fe. XANES and EXAFS analyses showed that sorbed-As(V)-goethite, sorbed-As(III)-ferrihydrite, scorodite and arsenopyrite were the predominant As species in the mine spoil. As(V) dominated at oxic conditions and As(III) predominated at moderately reducing conditions, which may be attributed to either inability of arsenate bacteria to reduce As or incomplete reduction. Lower Fe/As molar ratios during moderately reducing conditions show that the mine spoil may migrate As to watercourses during flooding, which may increase the hazardous effects of this toxic element. Therefore, encouraging aerobic conditions may mitigate As release and potential loss from the mine field., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Arsenic (As) contaminated mining spoils pose health threats to environmental resources and humans, and thus, mitigating this potential risk is worth investigating. Here, we studied the impacts of biochar, compost, iron oxide, manure, and inorganic fertilizer on the non-specifically (readily bioavailable)- and specifically- sorbed As and soil quality improvement of an abandoned mine spoil highly contaminated with As (total As = 1807 mg/kg). Compost, iron oxide, manure, and biochar were each applied at 0.5%, 2%, and 5% (w/w) to the contaminated soil; and NPK fertilizer at 0.1, 0.2, and 5.0 g/kg. The non-specifically (readily bioavailable)- and specifically- sorbed As were extracted sequentially and available P, total C and N, dissolved organic carbon, soil soluble anions, and exchangeable cations were extracted after 1- and 28-day incubation. Compost, manure, and biochar at 5% improved the total C and N and exchangeable K + , Mg 2+ and Na + . However, manure, compost, and iron oxide at 5% reduced available P from 118.5 to 60.3, 12.6, and 7.1 mg/kg, respectively. As compared to the untreated soil, the addition of iron oxide doses reduced the readily bioavailable As by 93%; while compost, manure, inorganic fertilizers, and biochar increased it by 106-332%, 24-315%, 19-398%, and 28-47%, respectively, with a significantly higher impact for the 5% doses. Furthermore, compost reduced specifically-sorbed As content (14-37%), but the other amendments did not significantly affect it. The impacts of the amendments on the readily bioavailable As was stronger than on specifically-sorbed As; but these were not affected by the incubation period. Arsenic bioavailability in our soil increased with increasing the soil pH and the contents of Cl - , DOC, and exchangeable K + and Na + . We conclude that iron-rich materials can be used to reduce As bioavailability and to mitigate the associated environmental and human health risk in such mining spoils. However, the carbon-, and P-rich and alkaline materials increased the bioavailability of As, which indicates that these amendments may increase the risk of As, but can be used to enhance phytoextraction efficiency of As in the gold mining spoil., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Integrated studies about potentially toxic elements (PTEs) in sites near gold mining spoils, their contamination and human health risk, as well as remediation potential of native plants are limited. Therefore, our aim was to assess the human health risk of PTEs (Al, As, Cd, Cr, Cu, Fe, Ni, Pb, Ti, V, and Zn) in sites near an abandoned gold mine spoil in Ghana. We collected 52 soil samples near the mine spoil and from a natural forest, determined their total element contents, and calculated the soil contamination factor (CF), enrichment factor (EF), geo-accumulation index (I geo ) and the pollution load index (PLI). In addition, we calculated the human health risk of soil ingestion for adult males, females, and children using the hazard quotient (HQ) and hazard index (HI). We also assessed the phytoremediation potential of five native plants (Alchornea cordifolia, Chromolaena odorata, Lantana camara, Pityrogramma calomelanos- fern, and Pueraria montana) growing near the mine spoil, and calculated their transfer coefficient (TC) and translocation factor (TF). Total content of As (maximum: 3144.0 mg/kg) surpassed the trigger action value of 65 mg/kg. Total Zn content at 90th percentile in the Pueraria field (197 mg/kg) and maximum value at mine surrounding (76.7 mg/kg) were above the world soil average (70 mg/kg). Pollution load index and EF values indicated severe levels of soil contamination particularly with As and Ti. Hazard index (HI) values for all sites for children (0.7-134.56), adult males (0.05-10.6), and adult females (0.13-12.77) were above 1 and indicated high human health risk especially on children and women. Translocation factor shows that native plant species such as Chromolaena odorata and fern accumulated As, Cu, Ti, and Zn into their shoots and may thus have the potential to reduce the high soil contamination and its associated human health risk., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

This work aims to study the pseudo-total content, geochemical fractions, and species of arsenic (As) in the bulk soil and in the coarse and fine particles of top soil and soil profiles collected from active and abandoned gold mine spoils in Ghana. The human health risk for adults (male and female) and children has been assessed. To achieve our aims, we collected 51 samples, characterized them, determined the total As content, and sequentially extracted the geochemical fractions of As including water-soluble and un-specifically bound As (FI); specific-sorbed/exchangeable As (FII); poorly (FIII)- and well-crystalline (IV) Fe oxide; and residual/sulphide fraction (FV). In selected samples, As species were determined using synchrotron-based X-ray absorption near edge structure (XANES). Pseudo-total As contents varied from 1807 to 8400 mg kg -1 , with the extremes occurring at the abandoned mine spoil. Arsenic was almost 10-fold higher in the fine particles (<0.63 μm) than in the coarse particles. Arsenic was mainly associated with FIII and FV, indicating that the distribution of As in these spoils is governed by their contents of amorphous Fe oxides, sulphides and As bearing minerals. The XANES results indicated that scorodite (FeAsSO 4  = 65-76%) and arsenopyrite (FeAsS = 24-35%) are the two major As-containing minerals in the spoils. The potential mobility (PMF = ∑FI-FIV) of As in the fine particles of the top soil was higher (48-61%) than in the coarse particles (25-44%). The mobile fraction (MF) (FI+FII) and PMF of As in the coarse particles of the profiles increased with depth while it decreased in the fine particles. The median hazard index values indicated an elevated human health risk, especially for children. The high contamination degree and potential mobility of As at the studied mine spoils indicate high potential risk for human and environmental health., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Background: Despite the existence of an abortion law and a safe abortion policy in Ghana, the Ghana Statistical Service found that 15% of all women in the reproductive age group (15-49 years) have practiced unsafe abortions. The objective of this study was to explore factors that contribute to the high incidence of unsafe abortion practices in the Ashanti Region of Ghana., Methods: A qualitative descriptive study design was used to assess factors that contribute to unsafe abortion practices. Purposive sampling technique was employed in selecting participants. Data were collected through key informant interviews and focus group discussions. One hundred and eleven participants were involved in the study. Data analysis was carried out through qualitative content analysis., Results: Seven thematic categories were elicited from data collected. The categories are: a) Lack of knowledge of safe abortion services; b) Socio-economic conditions as a perceived influence for unsafe abortion practices; c) Safe abortion as a perceived religious and cultural taboo in Ghana; d) Stigma of unplanned pregnancy; e) A desire to bear children only after marriage; f) Avoiding parental/guardian disappointment and resentment; g) A desire to pursue education., Conclusions: Evidence available in this study suggests that several factors are responsible for unsafe abortion practices in Ghana. Lack of knowledge on safe abortion services, poor socio-economic conditions, cultural and religious beliefs, a stigma of unplanned pregnancy, a desire to bear children only after marriage, attempts to avoid parental/guardian disappointment and resentment, and a desire to pursue education were cited by participants as situations that contributed to unsafe abortion practices. Measures such as Aunty Jane, Ms. Rose and Women Help Women programmes can be publicised to reduce maternal morbidity and mortality that occur as a result of unsafe abortions in Ghana. Improvement in family planning education in educational institutions needs to be considered in order to reduce the rate of unwanted pregnancies among young women in school.

The present study looks at a paracentric inversion on chromosome 4 [inv(4)(q13;q25)] in members of a large schizophrenia kindred from Hong Kong, and the possibility of a susceptibility gene for schizophrenia at one of the inversion breakpoints. Fluorescence in situ hybridization with BAC and fosmid clones was used to determine the location of the 4q13 and 4q25 breakpoints, however bioinformatic analysis indicated that no known genes are directly disrupted by the breakpoints. We identified several putative genes and expressed sequence tags (ESTs) from around the breakpoint regions, and have characterized them further in order to determine whether they may represent full-length mRNAs that are disrupted by the inversion. Overall, it appears that, while no known genes are disrupted, an as yet undiscovered gene, or indeed, a known gene, may be present near one of the breakpoints and may be disrupted by position effect. We hypothesized that either the 4q13 or 4q25 breakpoint region may contain a common susceptibility gene for schizophrenia. We genotyped 117 schizophrenia families for several short tandem repeat polymorphisms close to the breakpoints. Family based association testing showed no association at the 4q13 breakpoint region, but showed significant allelic association for marker D4S2989 at the 4q25 breakpoint region (p=0.016). This study suggests that the 4q breakpoint regions may harbour a gene that contributes to the illness in the large Hong Kong pedigree, and this 4q25 region should be examined further in other schizophrenia samples.

A new splice variant of the Rett syndrome gene, MECP2, was recently identified, that includes coding sequence from exon 1, and is the predominant transcript in the central nervous system. This sequence encodes polyalanine and polyglycine stretches within the N-terminal portion of MeCP2, and may confer novel functional properties to the protein. We screened autism, mental retardation (MR), and control populations for sequence variation within this region, and identified variation in approximately 1% of MR cases screened (N = 1,410). No variants were identified in the autism sample (N = 401). Most of these variants occur within a trinucleotide repeat region and result in change in number of alanine or glycine residues within the repeat stretches. We suggest some of these variants may be a relatively frequent cause of non-specific MR or developmental delay., ((c) 2006 Wiley-Liss, Inc.)

Objective: To determine whether the circadian rhythm of circulating levels of insulin-like growth factor binding protein-1 (IGFBP-1) is evident at the time of delivery., Design: Prospective observational study in six pregnant women and cross-sectional study in 65 women at the time of delivery., Subjects: Six pregnant women sampled over a period of 24 hours, 23 women sampled at the time of elective caesarean section, and 42 women sampled at the time of vaginal delivery., Results: In the women sampled serially over a 24 hour period there was an obvious circadian rhythm of IGFBP-1 with a peak between 01.00 and 09.00 hours. In the women sampled at the time of delivery there was no evidence for any relationship between the time of delivery and maternal or fetal levels of IGFBP-1., Conclusions: The circadian rhythm of IGFBP-1 is not observed in women in labour. Therefore, the time of day is not an important confounding variable in studies on IGFBP-1 levels at the time of normal delivery.