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Background and Objectives:Increased antibiotic resistance as a result of using antimicrobial drugs and also their various adverse effects has caused that in recent years, investigation of medicinal plants beconsidered for discovering new medicinal resources against bacterial infections. This study was performed with the objective of determining the antimicrobial activity of aqueous and ethanolic extracts of the leaves of Kleussiaodoratissimaagainst Enterococcus faecalis (PTTC 1609) and Salmonella typhi (PTTC 1349). Methods:In this experimental study, the antibacterial activity of the extracts was investigated by spreading of the extract on medium surface and disk agar diffusion method. Minimum Inhibitory Concentration (MIC( and Minimum Bactericidal Concentration (MBC) were also determined using tube dilution method. Analysis of the results was carried out by one-way analysis of variance (ANOVA). Results: In agar disk diffusion method, all concentrations of ethanolic extract had inhibitory effect against Enterococcus faecalis. MIC of aqueous and ethanolic extracts for Enterococcus faecalis was 16 and 32mg/ml and for Salmonella typhiwas 64 and 32mg/ml, respectively.MBC of aqueous and ethanolic extracts for Enterococcus faecalis was 64 and 32mg/ml and for Salmonella typhi was 128 and 64mg/ml, respectively. Conclusion:The results showed that the ethanolic extract of Kelussiaodoratissima leave had higher inhibitory effects on the studied strains, compared to aqueous extracts in vitro.

Introduction & Objective: Teucrium polium belonging to Lamiaceae family has been widely used in traditional medicine for treatment of diseases. Regarding the existence of the active biological compounds in this plant, it seems that this plant has considerable antimicrobial ef-fects. The aim of this study is to investigate the antimicrobial effect of different concentra-tions of the aqueous and ethanolic extracts of Teucrium polium (different concentrations) on Escherichia coli PTCC 1330, Staphylococcus aureus PTCC 1337, Staphylococcus epider-midis PTCC 1435, Streptococcus pyogenes PTCC 1447 and Pseudomonas aeruginosa PTCC 1310” in vitro”. Materials & Methods: In this experimental study, antimicrobial effect of the extracts was ex-plored by two methods including “Collins method” (spreading of the extract on medium sur-face) and “disk agar diffusion method”. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using the dilution method. Results: The results showed that in "disk agar diffusion method ", the ethanolic extract had the inhibitory effect on Staphylococcus epidermidis, Streptococcus pyogenes and Staphylococ-cus aureus. The MIC of the aqueous and ethanolic extracts for Pseudomonas aeruginosa was 64 and 32 mg/ml, respectively and the MBC of aqueous and ethanolic extracts for Pseudo-monas aeruginsa was 256 and 128 mg/ml, respectively. Pseudomonas aeruginosa showed the highest level of resistance against the aqueous and ethanolic Teucrium polium extracts. Conclusion: The ethanolic extract of Teucrium polium “in vitro” showed a considerable antim-icrobial effect on Pseudomonas aeruginosa as the gram-negative bacteria and Escherichia coli and Staphylococcus epidermidis, Streptococcus pyogenes and Staphylococcus aureus as the gram-positive bacteria.