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CCL22 is a macrophage-derived immunosuppressive chemokine that recruits regulatory T cells through the CCL22:CCR4 axis. CCL22 was shown to play a key role in suppressing anti-cancer immune responses in different cancer types. Recently, we showed that CCL22-specific T cells generated from cancer patients could kill CCL22-expressing tumor cells and directly influence the levels of CCL22 in vitro. The present study aimed to provide a rationale for developing a CCL22-targeting immunotherapy. Vaccination with CCL22-derived peptides induced CCL22-specific T-cell responses in both BALB/c and C57BL/6 mice, assessed by interferon-γ secretion ex vivo. Anti-tumor efficacy of the peptides was evaluated in mouse models engrafted with syngeneic tumor models showing a reduced tumor growth and prolonged survival of the treated mice. Vaccination induced changes in the cellular composition of immune cells that infiltrated the tumor microenvironment assessed with multicolor flow cytometry. In particular, the infiltration of CD8+ cells and M1 macrophages increased, which increased the CD8/Treg and the M1/M2 macrophage ratio. This study provided preclinical evidence that targeting CCL22 with CCL22 peptide vaccines modulated the immune milieu in the tumor microenvironment. This modulation led to an augmentation of anti-tumor responses. This study provided a rationale for developing a novel immunotherapeutic modality in cancer. [ABSTRACT FROM AUTHOR]

In the present paper we investigate soft skills in the light of metacognition. We seek the essential soft skills in the 21st century including green skills and look into their cognitive and metacognitive background. Enlightening the soft skills' dependence on metacognition, we conclude on a metacognitionbased approach and suggest useful tools and strategies. The metacognitive approach of soft skills can be applied in a variety of educational contexts as a training paradigm to accelerate the inclusion and success of students, employees and citizens, especially those belonging in vulnerable groups like persons with disabilities. [ABSTRACT FROM AUTHOR]

ABSTRACT Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (Ritter et al., 2016, Biotechnol Bioeng). Here, we performed knock-down and knock-out experiments of genes located within this telomeric region of chromosome 8 to identify the genes causing the observed phenotypes of CHO-C8DEL cell lines. We present evidence that loss or reduced expression of the gene C12orf35 is responsible for higher productivities and shorter recovery times during selection pressure. These effects are mediated by increased levels of mRNA of the exogenes heavy chain (HC) and light chain (LC) as well as dihydrofolate reductase (DHFR) and neomycin phosphotransferase (Neo) during the stable expression of antibodies. Biotechnol. Bioeng. 2016;113: 2433-2442. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Touristic destinations all around the world are struggling to digitally transform the touristic experience and the touristic products they offer and to capitalize a good experience with new tourists and returning ones. There is a lot of research on digital solutions assisting tourism, but it does not provide a follow-up digital product, therefore depending only on physical gifts, postcards and mementos. In this work, we propose a novel platform that can provide a personalized digital memento or digital gift for every route or tourist destination that can become the digital point of reference of visitors' experience giving new dimensions for commercialization to the existing physical mementos at the gift shops. The purpose of this study is to analyze what comprises a memorable touristic experience and to design and, finally, present a total solution that builds and offers a personal e-souvenir of a touristic experience to the tourist for him to hold, sport and share just using his mobile phone. We propose a digital memento-building platform that includes the personalized experience in visits taking place while in vacations. The visitors are usually taking pictures along routes they follow that later need further organization and processing and that in no way substitutes the physical mementos. In our approach, we propose a solution that generates a unique and personalized e-souvenir through a collage of the selfies and photos creating the digital equivalent of the touristic postcards but in our case personalized with the visitor photos with minimum amount of effort and produced real-time. Our approach is also providing a platform to the photographers and designers of touristic destinations to build and graphically generated memento artifacts—templates specific for one or more destinations or routes. In this way, the approach serves the tourism industry vertically, covering all aspects, i.e., the tourist-visitor, the tourism professional players such as photographers, designers of physical mementos and, of course, the touristic destination providing a digital footprint to server marketing of the destination through sharing on social media and word-of-mouth, of course. To support our approach, we have built and run a fully working prototype in the touristic center of Athens, Greece, with real users and designers for several weeks during summer vacations. The results have been greatly encouraging from end-users and professionals. The study shows that it is possible for various lines of business to come together and work along one another for an improve touristic experience using mobile technologies in a personalized, targeted approach. The touristic destination, graphic designers, photographers, tourist agent specialists, software developers and visitors can all now have a digital personalized memorable gift from the visit. [ABSTRACT FROM AUTHOR]

Computational experiment approach considers modelling as the essential feature of Inquiry Based Science Education (IBSE), where the model and the computer take the place of the “classical” experimental set-up and simulation replaces the experiment (Landau, Pαez, & Bordeianu, 2008). Modelling, as a pedagogical tool, involves the model construction, the exploration of model characteristics and the model application to a specific problem, resembling authentic activities of scientists and mathematicians (Herbert, 2003). Recent developments in strategy instruction research suggest that learning in a particular discipline is enhanced by guiding students through the development of content-relevant metacognitive strategies (Wosnitza & Volet, 2009). Problem-solving is a complex process, which involves several cognitive operations such as collecting and selecting information, heuristic strategy and metacognition (De Corte, 2003; Garofalo & Lester, 1985; Schoenfeld, 1994). The purpose of this study was to explore the impact of the Computational Experiment Methodology on learners' cognitive performance, use of modelling indicators and shift of the metacognitive experiences during problem solving using computational models. Sixty prospective primary school teachers volunteered to participate in the study. Students were exposed by the Instructor to a number of computational experiments, while during the course they developed their own models of simulation. The results of the experiment show that the use of the computational experiment approach has a substantial effect on the metacognitive experiences and the use of modelling indicators. [Copyright &y& Elsevier]