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Your search keyword '"Mann, Kenneth G."' showing total 324 results
Start Over Author "Mann, Kenneth G." Publication Type Academic Journals
324 results on '"Mann, Kenneth G."'

1. Assessing Factor V Antigen and Degradation Products in Burn and Trauma Patients.

Author

Keyloun, John W, Le, Tuan D, Orfeo, Thomas, Brummel-Ziedins, Kathleen E, Bravo, Maria C, Kaye, Matthew D, Bourne, Dana E, Carney, Bonnie C, Freeman, Kalev, Mann, Kenneth G, Pusateri, Anthony E, Shupp, Jeffrey W, and SYSCOT Study Group

Subjects
SYSCOT Study Group, Humans, Blood Coagulation Disorders, Burns, Factor V, Factor Va, Injury Severity Score, Coagulopathy, Protein C, Trauma, Physical Injury - Accidents and Adverse Effects, Clinical Research, Clinical Sciences, Surgery
Abstract

IntroductionProposed mechanisms of acute traumatic coagulopathy (ATC) include decreased clotting potential due to factor consumption and proteolytic inactivation of factor V (FV) and activated factor V (FVa) by activated protein C (aPC). The role of FV/FVa depletion or inactivation in burn-induced coagulopathy is not well characterized. This study evaluates FV dynamics following burn and nonburn trauma.MethodsBurn and trauma patients were prospectively enrolled. Western blotting was performed on admission plasma to quantitate levels of FV antigen and to assess for aPC or other proteolytically derived FV/FVa degradation products. Statistical analysis was performed with Spearman's, Chi-square, Mann-Whitney U test, and logistic regression.ResultsBurn (n = 60) and trauma (n = 136) cohorts showed similar degrees of FV consumption with median FV levels of 76% versus 73% (P = 0.65) of normal, respectively. Percent total body surface area (TBSA) was not correlated with FV, nor were significant differences in median FV levels observed between low and high TBSA groups. The injury severity score (ISS) in trauma patients was inversely correlated with FV (ρ = -0.26; P = 0.01) and ISS ≥ 25 was associated with a lower FV antigen level (64% versus. 93%; P = 0.009). The proportion of samples showing proteolysis-derived FV was greater in trauma than burn patients (42% versus. 16%; P = 0.0006).ConclusionsIncreasing traumatic injury severity is associated with decreased FV antigen levels, and a greater proportion of trauma patient samples exhibit proteolytically degraded FV fragments. These associations are not present in burns, suggesting that mechanisms underlying FV depletion in burn and nonburn trauma are not identical.

Published
2022

2. Inhalation Injury is Associated with Endotheliopathy and Abnormal Fibrinolytic Phenotypes in Burn Patients: A Cohort Study

Author

Keyloun, John W, Le, Tuan D, Brummel-Ziedins, Kathleen E, Mclawhorn, Melissa M, Bravo, Maria C, Orfeo, Thomas, Johnson, Laura S, Moffatt, Lauren T, Pusateri, Anthony E, Shupp, Jeffrey W, McLawhorn, Melissa M, Callcut, Rachael A, Cohen, Mitchell J, Petzold, Linda R, Varner, Jeffrey D, Bravo, Maria Cristina, Freeman, Kalev, Mann, Kenneth G, Gautam, Aarti, Hammamieh, Rasha, and Jett, Marti

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Hematology, Physical Injury - Accidents and Adverse Effects, Clinical Research, Good Health and Well Being, Burns, Cohort Studies, Female, Humans, Male, Phenotype, Retrospective Studies, Thrombelastography, SYSCOT Study Group, Emergency & Critical Care Medicine, Clinical sciences, Allied health and rehabilitation science, Nursing
Abstract

Burn injury is associated with endothelial dysfunction and coagulopathy and concomitant inhalation injury (IHI) increases morbidity and mortality. The aim of this work is to identify associations between IHI, coagulation homeostasis, vascular endothelium, and clinical outcomes in burn patients. One hundred and twelve patients presenting to a regional burn center were included in this retrospective cohort study. Whole blood was collected at set intervals from admission through 24 hours and underwent viscoelastic assay with rapid thromboelastography (rTEG). Syndecan-1 (SDC-1) on admission was quantified by ELISA. Patients were grouped by the presence (n = 28) or absence (n = 84) of concomitant IHI and rTEG parameters, fibrinolytic phenotypes, SDC-1, and clinical outcomes were compared. Of the 112 thermally injured patients, 28 (25%) had IHI. Most patients were male (68.8%) with a median age of 40 (interquartile range, 29-57) years. Patients with IHI had higher overall mortality (42.68% vs 8.3%; P < .0001). rTEG LY30 was lower in patients with IHI at hours 4 and 12 (P < .05). There was a pattern of increased abnormal fibrinolytic phenotypes among IHI patients. There was a greater proportion of IHI patients with endotheliopathy (SDC-1 > 34 ng/ml) (64.7% vs 26.4%; P = .008). There was a pattern of increased mortality among patients with IHI and endotheliopathy (0% vs 72.7%; P = .004). Significant differences between patients with and without IHI were found in measures assessing fibrinolytic potential and endotheliopathy. Mortality was associated with abnormal fibrinolysis, endotheliopathy, and IHI. However, the extent to which IHI-associated dysfunction is independent of TBSA burn size remains to be elucidated.

Published
2022

9. TACTIC: Trans‐Agency Consortium for Trauma‐Induced Coagulopathy

Author

Mann, K.G., Freeman, K., Mann, Kenneth G., Esmon, Charles T., Wisnewski, Stephen, Tracy, Russell P., Kindzelski, Andrei L., Pusateri, Anthony, Banerjee, Anirban, Brass, Lawrence F., Brummel‐Ziedins, Kathleen E., Butenas, Saulius, Cohen, Mitchell J., Diamond, Scott L., Freeman, Kalev, Moore, Ernest E., Morrissey, James H., Nelson, Mark T., Park, Myung S., Ruf, Wolfram, Shupp, Jeffrey W., Sperry, Jason L., Spiess, Bruce D., Stalker, Timothy J., and Zuckerbraun, Brian S.

Published
2015
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36. Self-association of human protein S

Author

Pauls, Jonathan E.D., Hockin, Matthew F., Long, George L., and Mann, Kenneth G.

Subjects
Biochemistry -- Research, Protein S -- Research, Calcium -- Research, Coagulation -- Analysis, Biological sciences, Chemistry
Abstract

Research has been conducted on the human protein S. Experiments indicate that this protein reversibly self-associates in the absence of Ca (super)2+.

Published
2000

37. Age-related phenotypic alterations in populations of purified human bone precursor cells

Author

Long, Michael W., Ashcraft, Elizabeth K., Normalle, Daniel, and Mann, Kenneth G.

Subjects
Bone cells -- Research, Bones -- Growth, Osteoblasts -- Research, Health, Seniors
Abstract

Research indicates that human bone cell phenotypic markers exhibit distinct age-related alterations, with some elderly people demonstrating significantly lower quantities of bone proteins. Immunologically purified bone preosteoblast-like cell populations were used in the research, with the findings facilitating a better understanding of the early stages of bone cell development.

Published
1999

42. Relation of coagulation parameters to patency and recurrent ischemia in the Thrombolysis in Myocardial Infarction (TIMI) Phase II Trial

Author

Tracy, Russell P., Kleiman, Neal S., Thompson, Bruce, Cannon, Christopher P., Bovill, Edwin G., Brown, R. Greg, Collen, Desire, Mahan, Edward, Mann, Kenneth G., Rogers, William J., Sopko, George, Stump, David C., Williams, David O., and Zaret, Barry L.

Subjects
Blood coagulation factors -- Physiological aspects, Thrombolytic therapy -- Usage, Heart attack -- Development and progression, Coronary heart disease -- Longitudinal studies, Health
Published
1998

45. Analysis of tissue plasminogen activator specificity using peptidyl fluorogenic substrates

Author

Butenas, Saulius, Kalafatis, Michael, and Mann, Kenneth G.

Subjects
Tissue plasminogen activator -- Research, Structure-activity relationships (Biochemistry) -- Analysis, Biological sciences, Chemistry
Abstract

Quantitative evaluation of tissue-type plasminogen activator (tPA) was done to evaluate substrate structure-efficiency correlations and to assess the specificity of tPA in hydrolyzing peptidyl fluorogenic substrates. Synthetic substrates used in the experiment contained 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, Arg in the P1 position, various amino acids in the P2 and P3 positions, and different substituents in the sulfonamide moiety in the leaving group. The results showed that the factors that affect the efficiency and affinity of the substrates for tPA include hydrophobicity of the substrates, the volume of amino acids in the peptide core and the structure of the leaving group.

Published
1997

47. Kinetics of human factor VII activation

Author

Butenas, Saulius and Mann, Kenneth G.

Subjects
Proteins -- Research, Thrombin -- Physiological aspects, Biological sciences, Chemistry
Abstract

The membrane bound factor Xa appears to be the major activator of the vitamin K dependent zymogen, human factor VII, in vivo. The activation by Xa is anionic phospholipid (PCPS) dependent. The VIIa-tissue factor complex (factor IXa) and thrombin activate factor VII in vitro, but in vivo activation is uncertain. In the presence of PCPS, factor VIIIa has no affect on the activation by factor IXa, while factor Va decreases the activation by Xa. Factor XIa has no observable effect on factor VII activation rates.

Published
1996

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