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The optimal conditions were explored for the preparation of Spirulina blended liquor (SBL) and Spirulina fermented wine (SFW), respectively. The parameters obtaining highest alga polysaccharide were calculated by response surface methodology. The optimal conditions for SBL preparation were base liquor of 42% vol, ultrasonication time of 37‐min and ultrasonic power of 80 W with polysaccharide content (PC) and alcohol content (AC) of 0.2181 g/L and 39.7% vol, respectively. In the case of SFW, optimum fermentation occurred at 22°C, with a 4% inoculum and 6‐day period with PC and AC of 8.533 g/L and 11.2% vol, respectively. Headspace solid‐phase microextraction–gas chromatography–mass spectrometry was used to quantitatively analyze the volatile components of SBL and SFW. There were 32 and 40 main aroma compounds in SBL and SFW, respectively. Volatile organic compounds, including α‐ionone and β‐ionone, produced by Spirulina were detected in both SBL and SFW. Comparative evaluation of scavenging activity and total reducing power revealed the antioxidant capacity of SFW significantly outperformed that of SBL. [ABSTRACT FROM AUTHOR]

The adaptive evolution and coevolution of the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit ( rbc L) gene in the genus Hildenbrandia were studied based on phylogenetic tree construction and the physicochemical properties and the secondary structures of protein encoded by rbc L (Rubisco large subunit) were analyzed. The amino acids compositions and grand average of hydropathicity of freshwater H. rivularis and marine H. rubra were similar. Rubisco large subunit of Hildenbrandia was hydrophilic and the secondary structure was primarily composed of α-helixes and β-sheets, revealing the relatively stable structure of this protein. The predicted phosphorylation sites in H. rivularis and H. rubra were 33 and 36, respectively. No positive selection sites were detected in the genus Hildenbrandia , implying that rbc L gene evolved either neutrally or under purifying selection. A total of 41 coevolutionary groups were detected in the Rubisco large subunit of Hildenbrandia and the coevolving sites are in closer proximity in 3-dimensional structure of the protein. Despite the long evolutionary history, rbc L gene in genus Hildenbrandia under different environments is rather conservative.

Land‐use change has significant influences on soil respiration (Rs) in terrestrial ecosystems. Soil microbes play critical roles in soil carbon cycling. Nevertheless, the specific mechanism of how changes in soil microbial properties are linked to the variation of Rs rate during land‐use change still remains poorly understood, especially in the Loess Plateau, China. Here, the characteristics of Rs rate and soil microbial community following the land‐use change from farmland to plantation/grassland were analyzed via an automated soil CO2 flux system and high‐throughput 16S rDNA gene sequencing. The afforestation altered soil microbial diversity and community composition, which was mainly explained by soil pH, temperature, organic matter, nitrate, alkali‐hydrolyzed nitrogen, and available phosphorus. The biomarkers of Bacteroidetes, Firmicutes, and Thaumarchaeota were found in farmland soil at the phylum level. The afforestation also significantly decreased Rs rate, which was closely related to Shannon's index, Simpson's index, and some microbial taxa, such as Bacteroidetes, Firmicutes, Nitrospirae, and Acidobacteria. Bacteroidetes, and Firmicutes were particularly expected to be important drivers of high Rs rate in farmland soil. Moreover, the microbial interaction was probably also an important factor affecting Rs rate. Our results indicate that the response of Rs to land‐use change depends on soil microbial community being regulated by soil physicochemical properties in the Loess Plateau, China. [ABSTRACT FROM AUTHOR]

Salt stress is one of the abiotic stress factors that affect the normal growth and development of higher plants and algae. However, few research studies have focused on calcium stress, especially in algae. In this study, the mechanism of tolerance to high calcium stress of a Parachlorella kessleri strain was explored by the method of transcriptomics combined with physiological and morphological analysis. Concentrations of CaCl2 100 times (3.6 g/L) and 1000 times (36 g/L) greater than the standard culture were set up as stresses. The results revealed the algae could cope with high calcium stress mainly by strengthening photosynthesis, regulating osmotic pressure, and inducing antioxidant defense. Under the stress of 3.6 g/L CaCl2, the algae grew well with normal cell morphology. Although the chlorophyll content was significantly reduced, the photosynthetic efficiency was well maintained by up-regulating the expression of some photosynthesis-related genes. The cells reduced oxidative damage by inducing superoxide dismutase (SOD) activities and selenoprotein synthesis. A large number of free amino acids were produced to regulate the osmotic potential. When in higher CaCl2 stress of 36 g/L, the growth and chlorophyll content of algae were significantly inhibited. However, the algae still slowly grew and maintained the same photosynthetic efficiency, which resulted from significant up-regulation of massive photosynthesis genes. Antioxidant enzymes and glycerol were found to resist oxidative damage and osmotic stress, respectively. This study supplied algal research on CaCl2 stress and provided supporting data for further explaining the mechanism of plant salt tolerance. [ABSTRACT FROM AUTHOR]

Batrachospermaceae is the largest family of freshwater red algae, widely distributed around the world, and plays an important role in maintaining the balance of spring and creek ecosystems. The deterioration of the current global ecological environment has also destroyed the habitat of Batrachospermaceae. The research on the environmental factors of Batrachospermaceae and the accurate classification of the genus is necessary for the protection, restoration, excavation, and utilization of Batrachospermaceae resources. In this paper, the database of geographical distribution and environmental factors of Batrachospermaceae was sorted out, and the relationship between the classification of genus and environmental factors in Batrachospermaceae was analyzed based on two machine learning methods, random forest and XGBoost. The result shows: (1) The models constructed by the two machine learning methods can effectively distinguish the genus of Batrachospermaceae based on environmental factors; (2) The overall AUC score of the random forest model for the classification and prediction of the genus of Batrachospermaceae reached 90.41%, and the overall AUC score of the taxonomic prediction of each genus of Batrachospermaceae reached 85.85%; (3) Combining the two methods, it is believed that the environmental factors that affect the distinction of the genus of Batrachospermaceae are mainly altitude, average relative humidity, average temperature, and minimum temperature, among which altitude has the greatest influence. The results can further clarify the taxonomy of the genus in Batrachospermaceae and enrich the research on the differences in environmental factors of Batrachospermaceae. [ABSTRACT FROM AUTHOR]

Three species of the genus Synura from China are described here. Morphological observations and molecular phylogenetic analyses were conducted for three specimens collected at different locations in China. The described morphological features included cell size, scale size, spines, keels, and struts. Molecular analyses based on multiple genetic markers (SSU and LSU rDNA and internal transcribed spacer rDNA) were used to determine the phylogenetic positions of the three Synura species. Morphologically, specimen GZ201017 collected in Guizhou Province was characterized by a well-developed keel and lanceolate scales; specimen SX210304 collected in Shanxi Province was characterized by a less-developed keel and poor silicification; and specimen GD201126 collected in Guangdong Province was characterized mainly by spines with blunt ends or two small teeth on the tips. The morphotypes GZ201017, SX210304, and GD201126 corresponded to the original descriptions of Synura petersenii, S. glabra, and S. longitubularis, respectively. This discovery laid a foundation for the molecular phylogeny of the genus Synura and an enhanced understanding of Synura diversity and distribution in China. [ABSTRACT FROM AUTHOR]