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Light pollution (LP), induced by human activities, has become a crucial threat to biodiversity on the Tibetan plateau (TP), but few studies have explored its coverage and dynamics. In this study, we intended to measure the spatiotemporal patterns of LP on the TP from 1992 to 2018. First, we extracted the annual extent of LP from time-series nighttime light data. After that, we analyzed its spatiotemporal patterns at multiple scales and identified the natural habitats and the species habitats affected by LP. Finally, we discussed the main influencing factors of LP expansion on the TP. We found that the LP area increased exponentially from 1.2 thousand km2 to 82.8 thousand km2, an increase of nearly 70 times. In 2018, LP accounted for 3.2% of the total area of the TP, mainly concentrated in the eastern and southern areas. Several national key ecological function zones (e.g., the Gannan Yellow river key water supply ecological function zone) and national nature reserves (e.g., the Lalu Wetland National Nature Reserve) had a large extent of LP. The proportion of LP area on natural habitats increased from 79.6% to 91.4%. The number of endangered species with habitats affected by LP increased from 89 to 228, and more than a quarter of the habitats of 18 endangered species were affected by LP. We also discovered that roadways as well as settlements in both urban and rural areas were the main sources of LP. Thus, to lessen LP's negative effects on biodiversity, effective measures should be taken during road construction and urbanization on the TP. [ABSTRACT FROM AUTHOR]

Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicle development. Morphological analysis showed that treatment of E2 at 2.5 mg/kg increased the number of growing follicles by 28. 5% and activated follicles by 8.9%, compared with the group. Treatment of E2 also led to increased diameter and area of three kinds of follicles and oocytes, respectively. However, this stimulating effect of E2 was inhibited by combined treatment of the estrogen receptor antagonist tamoxifen, resulting in decreased number of the growing follicles by 14.3% and the activated follicles by 5.8%, respectively. Immunohistochemical staining of the proliferating cell nuclear antigen (PCNA) revealed that the ratios of the PCNA-positive granulosa and interstitial cells were increased by 14.0% and 21.5%, respectively. Western blot analysis of PCNA confirmed this stimulating effect of E2. Expression of ERα mRNAs was elevated by administration of E2 in vivo and in vitro. Furthermore, E- and N-cadherin displayed increased expression after E2 treatment in vivo and in vitro. In conclusion, E2 can promote chicken primordial follicle development and activation involving its increased receptor and cadherin production at the early stage of ovarian development. [ABSTRACT FROM AUTHOR]

Background: Long non-coding RNA (lncRNA) plays a significant role in the development, establishment, and progression of head and neck squamous cell carcinoma (HNSCC). This article aims to develop an immune-related lncRNA (irlncRNA) model, regardless of expression levels, for risk assessment and prognosis prediction in HNSCC patients. Methods: We obtained clinical data and corresponding full transcriptome expression of HNSCC patients from TCGA, downloaded GTF files to distinguish lncRNAs from Ensembl, discerned irlncRNAs based on co-expression analysis, distinguished differentially expressed irlncRNAs (DEirlncRNAs), and paired these DEirlncRNAs. Univariate Cox regression analysis, LASSO regression analysis, and stepwise multivariate Cox regression analysis were then performed to screen lncRNA pairs, calculate the risk coefficient, and establish a prognosis model. Finally, the predictive power of this model was validated through the AUC and the ROC curves, and the AIC values of each point on the five-year ROC curve were calculated to select the maximum inflection point, which was applied as a cut-off point to divide patients into low- or high-risk groups. Based on this methodology, we were able to more effectively differentiate between these groups in terms of survival, clinico-pathological characteristics, tumor immune infiltrating status, chemotherapeutics sensitivity, and immunosuppressive molecules. Results: A 13-irlncRNA-pair signature was built, and the ROC analysis demonstrated high sensitivity and specificity of this signature for survival prediction. The Kaplan-Meier analysis indicated that the high-risk group had a significantly shorter survival rate than the low-risk group, and the chi-squared test certified that the signature was highly related to survival status, clinical stage, T stage, and N stage. Additionally, the signature was further proven to be an independent prognostic risk factor via the Cox regression analyses, and immune infiltrating analyses showed that the high-risk group had significant negative relationships with various immune infiltrations. Finally, the chemotherapeutics sensitivity and the expression level of molecular markers were also significantly different between high- and low-risk groups. Conclusion: The signature established by paring irlncRNAs, with regard to specific expression levels, can be utilized for survival prediction and to guide clinical therapy in HNSCC., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Yin, Li, Lv, He, Luo, Li and Hu.)

To explore the effects of Qing Qiao Capsule in the treatment of chronic secretory otitis media and the levels of serum inflammatory factors, a total of 50 chronic secretory otitis media patients in the control group were subjected to caefaclor capsule, while the total of 50 cases in the observation group were treated with Qing Qiao Capsule. The traditional Chinese medicine (TCM) syndrome scores, therapeutic effects, and the levels of inflammatory factors were evaluated. After treatment, the scores of deafness, hearing loss, dizziness, soreness and weakness of the waist and knees, and fever is hens in palms and soles were significantly decreased in both groups (all P value <0.05). However, each score in the observation group was markedly less than that of the control group (all P value <0.05). Moreover, the C-reactive protein (CRP), procalcitonin (PCT) and tumor necrosis factor-α (TNF-α) levels measured after treatment were significantly lowered than those before treatment (all P value <0.05). Also, the levels of CRP, PCT and TNF-α in the observation group were obviously less than that of the control group (all P value <0.05). And the total therapeutic efficacy of the observation group was significantly higher than that of the control group (P<0.05). But no significant difference was observed in the rates of adverse reactions between both groups (P>0.05). Application of Qing Qiao Capsule in the treatment of chronic secretory otitis media yields better results, lowers TCM syndrome scores, and alleviates the body's inflammatory response, which is a safe drug in clinical use.