Search

With the development of cloud-network resource transaction technology based on smart contracts, computing power transactions have reached a multi-party scale, seriously affecting the efficiency and credibility of computing network resource transactions. To meet the requirements of trusted transactions and efficient resource incentives in the scenario of multi-party participation in computing network resources, a blockchain-based trusted identity authentication and resource transaction incentive mechanism for computing network resources was designed. The mechanism adopted a distributed authentication strategy based on the reliability of user identity information of the computing network and constructed a trusted transaction entrustment certificate through the authorization of an impartial agency, realizing the trusted implementation of resource transaction tasks. In addition, the application of the transaction incentive mechanism based on contract theory in the trusted transaction and efficient service of computing network resources was discussed. The mechanism was compared with linear incentive and trust incentive models, and the resource utility income and social welfare of the computing network participants were compared. The multi-party optimal utility benefit incentive was verified to measure the incentive efficiency of the mechanism. It proved the effectiveness of the resource transaction incentive mechanism in the transaction verification scenario and effectively ensured the trusted transaction and efficient incentive of computing network resources accessed by multiple service parties. Experimental results show that the proposed transaction mechanism could ensure the credibility, verifiability, and stability of multi-party computing network user transactions. The proposed mechanism is superior to the traditional transaction incentive mechanism in terms of resource transaction efficiency and social welfare benefits.

In this paper, the HSV color space model is used to collect the color characteristics of modern interior design and classify them into three types: hue (H), saturation (S), and luminance (V). At the same time, it combines with the theory of color psychology to construct a PAD emotion model for interior design. Then, it selects experimental samples and data analysis tools to carry out an example analysis of interior design from the emotional perspective. The data show that the selection of people warm and bright color interior design in the interior design of the background color across the amplitude of the largest and more concentrated in the 47.5°, the main body color across the amplitude of the smallest and most stable, the accent color value of the whole relatively high, the highest 123.6°, the background color saturation is the highest of 34.6°, and the brightness of more than 63.9°, the overall presentation of the warmth of comfort indoor environment. Error-values were 0.004, 0.0698, and 0.038 in order, with the errors all below 10%. This study integrates psychology and color into interior emotional design to better create visually appropriate, comfortable, and culturally rich interior environments for people, and also to promote the development of the interior design field.

In this paper, the synthesis of ginsenoside Rh2 was catalyzed by using heterologous expression of Bacillus subtilis SL-44 glycosyltransferase. The synthesis parameters of ginsenoside Rh2 were optimized by the selection of strains and chemical supplies, the establishment of kinetic equations for the respiration rate of UGT enzyme, the effect of storage temperature on the model, and the glycosylation reaction of ginsenoside PPD with UGT. The effect of Rh2 saturation on the thermal denaturation temperature of the protein was analyzed along with the kinetic properties of the enzyme GE02773 (GE03484) while varying the saturation of Rh2. The results showed that the conversion of ginsenoside Rh2 reached 84% at a temperature of 35℃, pH 8, 5% DMSO, 0.4 of M-UDPG, and 1M-PPD in reaction with GE02773. In this paper, we successfully achieved the efficient synthesis of ginsenoside Rh2, which provides new ways and ideas for the application and synthesis of ginsenoside Rh2, with important practical significance and scientific value.

Hyperglycemia drives dysfunction of the intestinal barrier. 5-Hydroxytryptaine 4 receptor (5-HT4R) agonists have been considered therapeutics for constipation in clnic. However, the roles of 5-HT4R activation in mucosa should be fully realized. Here, we investigate the effects of 5-HT4R activation on diabetes-induced disruption of the tight junction (TJ) barrier in the colon. Not surprisingly, the TJ barrier in diabetic mice with or without 5-HT4R is tremendously destroyed, as indicated by increased serum fluorescein isothiocyanate (FITC)-dextran and decreased transepithelial electrical resistance (TER). Simultaneously, decreased expressions of TJ proteins are shown in both wild-type (WT) and 5-HT4R knockout (KO) mice with diabetes. Notably, chronic treatment with intraperitoneal injection of a 5-HT4R agonist in WT mice with diabetes repairs the TJ barrier and promotes TJ protein expressions, including occludin, claudin-1 and ZO-1, in the colon, whereas a 5-HT4R agonist does not improve TJ barrier function or TJ protein expressions in 5-HT4R KO mice with diabetes. Furthermore, stimulation of 5-HT4R inhibits diabetes-induced upregulation of myosin light chain kinase (MLCK), Rho-associated coiled coil protein kinase 1 (ROCK1), and phosphorylated myosin light chain (p-MLC), which are key molecules that regulate TJ integrity, in the colonic mucosa of WT mice. However, such action induced by a 5-HT4R agonist is not observed in 5-HT4R KO mice with diabetes. These findings indicate that 5-HT4R activation may restore TJ integrity by inhibiting the expressions of MLCK, ROCK1 and p-MLC, improving epithelial barrier function in diabetes.

Objective To investigate the effect of pathogenic bacterium-Porphyromonas gingivalis (P.g) on the proliferation and inflammatory factor expression of human colorectal cancer Caco-2 cells, to determine whether the Janus kinase 2-signal transducers and activators of transcription 3 (JAK2-STAT3) pathway is involved in the regulation of Caco-2 cell proliferation by P.g and to provide an experimental basis for further exploring the relationship between P.g and colorectal cancer. Methods Caco-2 cells were cultured in vitro, and P.g at different multiplicities of infection (MOIs) (0, 1, 10, 25) was selected to stimulate for 12, 24 and 48 h. The effect of P.g on the proliferation of Caco-2 cells was detected by CCK8. The stimulation time was set as 12, 24 and 48 h. MOI=0 was the control group, and MOI=1, 10 and 25 comprised the experimental group. qRT-PCR and Western blot were used to detect the changes in interleukin-6 (IL-6), interleukin-10(IL-10), JAK2 and STAT3 gene and protein (phosphorylated protein) levels in each group. Results After P.g infection of Caco-2 cells, P.g had a sustained stimulatory effect on the cells for 12, 24 and 48 h at MOI=1 and MOI=10 compared with the control group. Compared with that in the control group, the expression of pro-inflammatory factor IL-6 and related proliferative pathway protein JAK2 and STAT3 in Caco-2 cells with P.g infection increased in a concentration- and time-dependent manner (P