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Porcine reproductive and respiratory syndrome virus (PRRSV) has caused severe economic losses to the global swine industry. In recent years, the incidence of PRRSV-1 has been gradually increasing in China, but there are still few studies on it. In this study, clinical samples for PRRS virus isolation were collected from a pig farm in South China in 2022. We effectively isolated a strain of PRRSV utilizing PAM cells and demonstrated its consistent transmission capability on Marc-145 cells. The isolated strain was confirmed as PRRSV-1 by RT-qPCR, IFA, electron microscopy, etiolated spot purification and whole genome sequencing, the strain was named GD2022. The length of GD2022 genome is 15058nt; Based on the genome-wide genetic evolutionary analysis of GD2022, the strain was classified as PRRSV-1. Further genetic evolutionary analysis of its ORF5 gene showed that GD2022 belonged to PRRSV-1 subtype 1 and formed an independent branch in the evolutionary tree. Compared with the sequence of the classical PRRSV-1 strain (LV strain), GD2022 has several amino acid site mutations in the antigenic region from GP3 to GP5, these mutations are different from those of other PRRSV-1 strains in China. Recombination analysis showed no recombination events with GD2022. In addition, piglets infected with GD2022 displayed clinical respiratory symptoms and typical pathological changes. In this study, a strain of the PRRSV-1 virus was isolated using both PAM cells and Marc-145 and proved to be pathogenic to piglets, providing an important reference for the identification, prevention, and control of PRRSV-1.

OBJECTIVE:To quantitatively evaluate maxillary complete dentures fabricated from polylactic acid (PLA) using fused deposition modelling (FDM) technology. METHODS:A digital maxillary complete denture was prepared based on a standard maxillary edentulous plaster model. The PLA pattern was printed by a FDM machine, with 5 repetitions, while another 5 wax patterns were printed as control group, using a high accuracy three-dimensional (3D) wax printer. The patterns were scanned with a 3D scanner. A light-body silicone film was made after each denture pattern had been totally seated on the plaster model, and was scanned to determine its thickness, which reflected the 3D space between the plaster model and the tissue surface of the denture pattern. The overall area was separated into four parts: primary stress-bearing area, secondary stress-bearing area, border seal area and relief area, and the average deviation of these four parts were measured. The values were analyzed by independent t-test. RESULTS:The overall mean value and standard deviation of space between PLA denture patterns and plaster model was 0.277 ± 0.021 mm, while that of the wax denture patterns was 0.279 ± 0.045 mm, which showed a good fit overall. No statistically significant (𝑃 > 0.05) difference was observed between the PLA patterns and wax patterns. CONCLUSIONS:The adaptation of the PLA pattern of maxillary complete denture printed by FDM technology is comparable to that prepared by wax printer, and can satisfy the accuracy requirements.

PURPOSE. To improve the clinical effects of complete denture use and simplify its clinical application, a digital complete denture restoration workflow (Functional Suitable Digital Complete Denture System, FSD) was proposed and preliminary clinical evaluation was done. MATERIALS AND METHODS. Forty edentulous patients were enrolled, of which half were treated by a prosthodontic chief physician, and the others were treated by a postgraduate student. Based on the primary impression and jaw relation obtained at the first visit, diagnostic denture was designed and printed to create a definitive impression, jaw relation, and esthetic confirmation at the second visit. A redesigned complete denture was printed as a mold to fabricate final denture that was delivered at the third visit. To evaluate accuracy of impression made by diagnostic denture, the final denture was used as a tray to make impression, and 3D comparison was used to analyze their difference. To evaluate the clinical effect of FSD, visual analogue scores (VAS) were determined by both dentists and patients. RESULTS. Two visits were reduced before denture delivery. The RMS values of 3D comparison between the impression made via diagnostic dentures and the final dentures were 0.165 ± 0.033 mm in the upper jaw and 0.139 ± 0.031 mm in the lower jaw. VAS ratings were between 8.5 and 9.6 in the chief physician group, while 7.7 and 9.5 in the student group; there was no statistical difference between the two groups. CONCLUSION. FSD can simplify the complete denture restoration process and reduce the number of visits. The accuracy of impressions made by diagnostic dentures was acceptable in clinic. The VASs of both dentists and patients were satisfied. [ABSTRACT FROM AUTHOR]