11 results on '"Kaluzna, I."'
Search Results
2. Superhydrophobic functionalization of cutinase activated poly(lactic acid) surfaces.
- Author
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Gamerith, C., Orcal Yebra, A., Herrero Acero, E., Ortner, A., Guebitz, G. M., Pellis, A., Scaini, D., Kaluzna, I., Mink, D., and de Wildeman, S.
- Subjects
SUPERHYDROPHOBIC surfaces ,CUTINASE ,MICROFLUIDICS - Abstract
Superhydrophobic materials have focused the interest of many researchers due to their potential in a wide spectrum of applications like microfluidics or biosensors in the biomedical field. Typically, the increased surface roughness at the micro or nano scale needed for superhydrophobic surfaces is achieved by coating of different substances, which in combination with a lower surface energy lead to Water Contact Angle (WCA) values greater than 150°. Here, limited enzymatic surface hydrolyis poly(lactic acid) (PLA) was combined with spin coating of a steraic alkene ketene dimer (AKD) layer. The selective enzymatic hydrolysis creates, in a gentle and controlled way, new hydroxylic and carboxylic groups on the polymer surface without damaging the material bulk properties like alkaline treatment does. The creation of new hydrophilic surface groups lead to a significant increase in the hydrophilicity, decreasing the WCA to less than 30° while raising the roughness from an R
rms of 50.5 to 90.8 nm concomittantly increasing the exposed surface vs. the projected one by 13.2%. Coupling of PLA hydroxy groups with AKD was demonstrated by using a PLA model substrate and subsequent identification of the reaction product via LC-TOF/MS. On the PLA film, FTIR based detection of the characteristic β-ketoester bond peak between the AKD and enzymatically generated hydroxy groups on the surface confirmed successful coupling. Scanning Electron Microscopy (SEM) & Atomic Force Microscopy (AFM) imaging confirmed the presence of fractal structures after curation of the enzymatically activated PLA film. The suitable size, 4.15 μm on the lateral dimension and 0.7 μm on height of the structures, together with the high density of these fractal structures lead to a superhydrophobic surface (WCA >150°). This process represents an alternative to produce chemically inert superhydrophobic bio-based polyesters surfaces, by combining mild biocatalytic activation of a polyester film with non-toxic chemicals in an environmentally friendly manner. [ABSTRACT FROM AUTHOR]- Published
- 2017
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3. Prozessentwicklung für biokatalytische Hydroxylierungen unter Anwendung von Cytochrom P450 Monooxygenasen.
- Author
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Brummund, J., Kaluzna, I., Hilterhaus, L., Müller, M., and Liese, A.
- Published
- 2012
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4. Exploring Castellaniella defragrans Linalool (De)hydratase-Isomerase for Enzymatic Hydration of Alkenes.
- Author
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Engleder M, Müller M, Kaluzna I, Mink D, Schürmann M, Leitner E, Pichler H, and Emmerstorfer-Augustin A
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- Acyclic Monoterpenes, Alcohols chemistry, Alcohols metabolism, Alkenes chemistry, Catalysis, Escherichia coli metabolism, Hydro-Lyases chemistry, Hydrolysis, Isomerases, Monoterpenes chemistry, Alkenes metabolism, Betaproteobacteria metabolism, Hydro-Lyases metabolism, Monoterpenes metabolism
- Abstract
Acyclic monoterpenes constitute a large and highly abundant class of secondary plant metabolites and are, therefore, attractive low-cost raw materials for the chemical industry. To date, numerous biocatalysts for their transformation are known, giving access to highly sought-after monoterpenoids. In view of the high selectivity associated with many of these reactions, the demand for enzymes generating commercially important target molecules is unabated. Here, linalool (de)hydratase-isomerase (Ldi, EC 4.2.1.127) from Castellaniella defragrans was examined for the regio- and stereoselective hydration of the acyclic monoterpene β-myrcene to ( S )-(+)-linalool. Expression of the native enzyme in Escherichia coli allowed for identification of bottlenecks limiting enzyme activity, which were investigated by mutating selected residues implied in enzyme assembly and function. Combining these analyses with the recently published 3D structures of Ldi highlighted the precisely coordinated reduction-oxidation state of two cysteine pairs in correct oligomeric assembly and the catalytic mechanism, respectively. Subcellular targeting studies upon fusion of Ldi to different signal sequences revealed the significance of periplasmic localization of the mature enzyme in the heterologous expression host. This study provides biochemical and mechanistic insight into the hydration of β-myrcene, a nonfunctionalized terpene, and emphasizes its potential for access to scarcely available but commercially interesting tertiary alcohols.
- Published
- 2019
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5. Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca.
- Author
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Engleder M, Horvat M, Emmerstorfer-Augustin A, Wriessnegger T, Gabriel S, Strohmeier G, Weber H, Müller M, Kaluzna I, Mink D, Schürmann M, and Pichler H
- Subjects
- Amino Acid Sequence, Bioreactors, Glycosylation, Hydro-Lyases chemistry, Hydro-Lyases genetics, Hydro-Lyases isolation & purification, Kinetics, Nuclear Magnetic Resonance, Biomolecular, Pichia genetics, Recombinant Proteins genetics, Sequence Homology, Amino Acid, Spectrophotometry, Ultraviolet, Hydro-Lyases metabolism, Nectria enzymology
- Abstract
Kievitone hydratase catalyzes the addition of water to the double bond of the prenyl moiety of plant isoflavonoid kievitone and, thereby, forms the tertiary alcohol hydroxy-kievitone. In nature, this conversion is associated with a defense mechanism of fungal pathogens against phytoalexins generated by host plants after infection. As of today, a gene sequence coding for kievitone hydratase activity has only been identified and characterized in Fusarium solani f. sp. phaseoli. Here, we report on the identification of a putative kievitone hydratase sequence in Nectria haematococca (NhKHS), the teleomorph state of F. solani, based on in silico sequence analyses. After heterologous expression of the enzyme in the methylotrophic yeast Pichia pastoris, we have confirmed its kievitone hydration activity and have assessed its biochemical properties and substrate specificity. Purified recombinant NhKHS is obviously a homodimeric glycoprotein. Due to its good activity for the readily available chalcone derivative xanthohumol (XN), this compound was selected as a model substrate for biochemical studies. The optimal pH and temperature for hydratase activity were 6.0 and 35°C, respectively, and apparent Vmax and Km values for hydration of XN were 7.16 μmol min-1 mg-1 and 0.98 ± 0.13 mM, respectively. Due to its catalytic properties and apparent substrate promiscuity, NhKHS is a promising enzyme for the biocatalytic production of tertiary alcohols.
- Published
- 2018
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6. Semi-rational engineering of cytochrome CYP153A from Marinobacter aquaeolei for improved ω-hydroxylation activity towards oleic acid.
- Author
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Duan Y, Ba L, Gao J, Gao X, Zhu D, de Jong RM, Mink D, Kaluzna I, and Lin Z
- Subjects
- Electron Transport, Ferredoxins metabolism, Hydroxylation, Marinobacter genetics, Mutagenesis, Site-Directed, Mutant Proteins genetics, Mutant Proteins metabolism, NADH, NADPH Oxidoreductases metabolism, Cytochrome P-450 Enzyme System genetics, Cytochrome P-450 Enzyme System metabolism, Marinobacter enzymology, Oleic Acid metabolism, Protein Engineering
- Abstract
ω-Hydroxy oleic acid is an important intermediate for the synthesis of certain polyesters and polyamides. In this study, a functional CYP153A/putidaredoxin (Pdx)/putidaredoxin reductase (Pdr) hybrid system was engineered for improved ω-hydroxylation activity towards oleic acid. By the combination of site-directed saturation mutagenesis (SDSM) and iterative saturation mutagenesis (ISM), a best mutant (Variant II) was obtained with mutations at two sites (S120 and P165) at the Pdx interaction interface with CYP153A, and one site (S453) in the substrate binding pocket. The in vitro-reconstituted activity of Variant II with purified Pdx and Pdr was 2.7-fold that of the template, while the whole cell transformation activity was 2.0-fold that of the template. A 96-well format-based screening scheme for CYP153A was also developed, which should be useful for engineering of other P450s with low activity. Kinetic analyses indicated that the activity improvement for CYP153A variants largely resulted from enhanced electron transfer. This further demonstrates the importance of the electron transfer between P450s and the non-native redox partners for the overall performance of hybrid P450 systems.
- Published
- 2016
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7. Process development for oxidations of hydrophobic compounds applying cytochrome P450 monooxygenases in-vitro.
- Author
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Brummund J, Müller M, Schmitges T, Kaluzna I, Mink D, Hilterhaus L, and Liese A
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- Cytochrome P-450 Enzyme System genetics, Enzyme Stability, Escherichia coli genetics, Hydrophobic and Hydrophilic Interactions, Models, Biological, NADP metabolism, Norisoprenoids metabolism, Oxidation-Reduction, Oxygen metabolism, Recombinant Proteins genetics, Cytochrome P-450 Enzyme System metabolism, Metabolic Engineering methods, Recombinant Proteins metabolism
- Abstract
Cytochrome P450 monooxygenases are a unique family of enzymes that are able to catalyze regio- and stereospecific oxidations for a broad substrate range. However, due to limited enzyme activities and stabilities, hydrophobicity of substrates, as well as the necessity of a continuous electron and oxygen supply the implementation of P450s for industrial processes remains challenging. Aim of this study was to point out key aspects for the development of an efficient synthesis concept for cytochrome P450 catalyzed oxidations. In order to regenerate the natural cofactor NADPH, a glucose dehydrogenase was applied. The low water soluble terpene α-ionone was used as substrate for the model reaction system. The studies reveal that an addition of surfactants in combination with low volumetric amounts of co-solvent can significantly increase substrate availability and reaction rates. Furthermore, these additives facilitated a reliable sampling procedure during the process. Another key factor for the process design was the oxygen supply. Based on various investigations, a bubble-aerated stirred tank reactor in batch mode represents a promising reactor concept for P450 oxidations. Main restriction of the investigated reaction system was the low process stability of the P450 monooxygenase, characterized by maximum total turnover numbers of ∼4100molα-ionone/molP450., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
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8. Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions.
- Author
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Wriessnegger T, Moser S, Emmerstorfer-Augustin A, Leitner E, Müller M, Kaluzna I, Schürmann M, Mink D, and Pichler H
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- Biotransformation, Culture Media, Cytochrome P-450 CYP2D6 genetics, Cytochrome P-450 CYP2D6 metabolism, Gene Expression Profiling, Humans, Hydrogen-Ion Concentration, Mentha piperita enzymology, Oxidation-Reduction, Pichia enzymology, Pichia growth & development, Rad52 DNA Repair and Recombination Protein metabolism, Sesquiterpenes metabolism, Terpenes metabolism, Up-Regulation, Cytochrome P-450 Enzyme System genetics, Cytochrome P-450 Enzyme System metabolism, Pichia genetics, Rad52 DNA Repair and Recombination Protein genetics
- Abstract
Cytochrome P450 enzymes (CYPs) play an essential role in the biosynthesis of various natural compounds by catalyzing regio- and stereospecific hydroxylation reactions. Thus, CYP activities are of great interest in the production of fine chemicals, pharmaceutical compounds or flavors and fragrances. Industrial applicability of CYPs has driven extensive research efforts aimed at improving the performance of these enzymes to generate robust biocatalysts. Recently, our group has identified CYP-mediated hydroxylation of (+)-valencene as a major bottleneck in the biosynthesis of trans-nootkatol and (+)-nootkatone in Pichia pastoris. In the current study, we aimed at enhancing CYP-mediated (+)-valencene hydroxylation by over-expressing target genes identified through transcriptome analysis in P. pastoris. Strikingly, over-expression of the DNA repair and recombination gene RAD52 had a distinctly positive effect on trans-nootkatol formation. Combining RAD52 over-expression with optimization of whole-cell biotransformation conditions, i.e. optimized media composition and cultivation at higher pH value, enhanced trans-nootkatol production 5-fold compared to the initial strain and condition. These engineering approaches appear to be generally applicable for enhanced hydroxylation of hydrophobic compounds in P. pastoris as confirmed here for two additional membrane-attached CYPs, namely the limonene-3-hydroxylase from Mentha piperita and the human CYP2D6., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
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9. Structure-Based Mechanism of Oleate Hydratase from Elizabethkingia meningoseptica.
- Author
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Engleder M, Pavkov-Keller T, Emmerstorfer A, Hromic A, Schrempf S, Steinkellner G, Wriessnegger T, Leitner E, Strohmeier GA, Kaluzna I, Mink D, Schürmann M, Wallner S, Macheroux P, Gruber K, and Pichler H
- Subjects
- Catalytic Domain, Molecular Structure, Flavobacteriaceae enzymology, Hydro-Lyases chemistry, Hydro-Lyases metabolism, Oleic Acid chemistry
- Abstract
Hydratases provide access to secondary and tertiary alcohols by regio- and/or stereospecifically adding water to carbon-carbon double bonds. Thereby, hydroxy groups are introduced without the need for costly cofactor recycling, and that makes this approach highly interesting on an industrial scale. Here we present the first crystal structure of a recombinant oleate hydratase originating from Elizabethkingia meningoseptica in the presence of flavin adenine dinucleotide (FAD). A structure-based mutagenesis study targeting active site residues identified E122 and Y241 as crucial for the activation of a water molecule and for protonation of the double bond, respectively. Moreover, we also observed that two-electron reduction of FAD results in a sevenfold increase in the substrate hydration rate. We propose the first reaction mechanism for this enzyme class that explains the requirement for the flavin cofactor and the involvement of conserved amino acid residues in this regio- and stereoselective hydration., (© 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.)
- Published
- 2015
- Full Text
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10. Over-expression of ICE2 stabilizes cytochrome P450 reductase in Saccharomyces cerevisiae and Pichia pastoris.
- Author
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Emmerstorfer A, Wimmer-Teubenbacher M, Wriessnegger T, Leitner E, Müller M, Kaluzna I, Schürmann M, Mink D, Zellnig G, Schwab H, and Pichler H
- Subjects
- Arabidopsis Proteins genetics, Basic Helix-Loop-Helix Transcription Factors genetics, Cytochrome P-450 Enzyme System chemistry, Cytochrome P-450 Enzyme System genetics, Fungal Proteins genetics, Pichia genetics, Protein Stability, Saccharomyces cerevisiae genetics, Sesquiterpenes metabolism, Up-Regulation genetics, Arabidopsis Proteins metabolism, Basic Helix-Loop-Helix Transcription Factors metabolism, Cytochrome P-450 Enzyme System metabolism, Fungal Proteins metabolism, Pichia metabolism, Saccharomyces cerevisiae metabolism
- Abstract
Membrane-anchored cytochrome P450 enzymes (CYPs) are a versatile and interesting class of enzymes for industrial applications, as they are capable of regio- and stereoselectively hydroxylating hydrophobic molecules. However, CYP activity requires sufficient levels of suitable cytochrome P450 reductases (CPRs) for regeneration of catalytic capacity, which is a bottleneck in many industrial applications. Searching for positive effectors of membrane-anchored CYP/CPR function, we transformed and screened selected strains from a Saccharomyces cerevisiae knockout collection for Hyoscyamus muticus premnaspirodiene oxygenase (HPO; CYP) and Arabidopsis thaliana CPR (AtCPR) expression levels, as well as for activity towards (+)-valencene. We found that in cells lacking the type III membrane protein Ice2p, AtCPR was destabilized. Remarkably, over-expression of ICE2 improved (+)-valencene hydroxylation to trans-nootkatol by 40-50%, both in resting cells and in vivo. Time-resolved immunoblot analysis and cytochrome c reductase activity assays revealed that Ice2 up-regulation stabilized AtCPR levels and activity over extended periods of bioconversion. To underscore that we had identified a novel positive effector of recombinant CYP/CPR function, we confirmed the beneficial effect of ICE2 over-expression for two further CYP/CPR combinations and the alternative host Pichia pastoris. Thus, we propose Ice2 up-regulation as a general tool for improving the applications of recombinant CYPs in yeasts., (Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
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11. Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris.
- Author
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Wriessnegger T, Augustin P, Engleder M, Leitner E, Müller M, Kaluzna I, Schürmann M, Mink D, Zellnig G, Schwab H, and Pichler H
- Subjects
- Arabidopsis enzymology, Arabidopsis genetics, Cupressus enzymology, Cupressus genetics, Hyoscyamus enzymology, Hyoscyamus genetics, Polycyclic Sesquiterpenes, Arabidopsis Proteins biosynthesis, Arabidopsis Proteins genetics, Metabolic Engineering, Pichia enzymology, Pichia genetics, Sesquiterpenes metabolism
- Abstract
The sesquiterpenoid (+)-nootkatone is a highly demanded and highly valued aroma compound naturally found in grapefruit, pummelo or Nootka cypress tree. Extraction of (+)-nootkatone from plant material or its production by chemical synthesis suffers from low yields and the use of environmentally harmful methods, respectively. Lately, major attention has been paid to biotechnological approaches, using cell extracts or whole-cell systems for the production of (+)-nootkatone. In our study, the yeast Pichia pastoris initially was applied as whole-cell biocatalyst for the production of (+)-nootkatone from (+)-valencene, the abundant aroma compound of oranges. Therefore, we generated a strain co-expressing the premnaspirodiene oxygenase of Hyoscyamus muticus (HPO) and the Arabidopsis thaliana cytochrome P450 reductase (CPR) that hydroxylated extracellularly added (+)-valencene. Intracellular production of (+)-valencene by co-expression of valencene synthase from Callitropsis nootkatensis resolved the phase-transfer issues of (+)-valencene. Bi-phasic cultivations of P. pastoris resulted in the production of trans-nootkatol, which was oxidized to (+)-nootkatone by an intrinsic P. pastoris activity. Additional overexpression of a P. pastoris alcohol dehydrogenase and truncated hydroxy-methylglutaryl-CoA reductase (tHmg1p) significantly enhanced the (+)-nootkatone yield to 208mg L(-1) cell culture in bioreactor cultivations. Thus, metabolically engineered yeast P. pastoris represents a valuable, whole-cell system for high-level production of (+)-nootkatone from simple carbon sources., (Copyright © 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)
- Published
- 2014
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