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Abstract Cancer is a disease with molecular heterogeneity that is closely related to gene mutations and epigenetic changes. The principal histological subtype of lung cancer is non‐small cell lung cancer (NSCLC). Long noncoding RNA (lncRNA) is a kind of RNA that is without protein coding function, playing a critical role in the progression of cancer. In this research, the regulatory mechanisms of lncRNA phosphorylase kinase regulatory subunit alpha 1 antisense RNA 1 (PHKA1‐AS1) in the progression of NSCLC were explored. The increased level of N6‐methyladenosine (m6A) modification in NSCLC caused the high expression of PHKA1‐AS1. Subsequently, high‐expressed PHKA1‐AS1 significantly facilitated the proliferation and metastasis of NSCLC cells, and these effects could be reversed upon the inhibition of PHKA1‐AS1 expression, both in vivo and in vitro. Additionally, the target protein of PHKA1‐AS1 was actinin alpha 4 (ACTN4), which is known as an oncogene. Herein, PHKA1‐AS1 could enhance the protein stability of ACTN4 by inhibiting its ubiquitination degradation process, thus exerting the function of ACTN4 in promoting the progress of NSCLC. In conclusion, this research provided a theoretical basis for further exploring the potential mechanism of NSCLC metastasis and searching novel biomarkers related to the pathogenesis and progression of NSCLC.

Malignant hyperthermia (MH) is an autosomal dominant genetic condition of the skeletal muscle triggered by inhaled general anesthetic agents or succinylcholine and associated with a hypermetabolic state and skeletal muscle rigidity. Tachycardia, increased carbon dioxide production, hypercarbia, hyperthermia, acidosis, hyperkalemia, cardiac arrhythmias, muscle rigidity, and rhabdomyolysis are common symptoms of MH. As the progression of the syndrome could be rapid or less evident, even experienced physicians have difficulty in diagnosing MH, which can lead to delays in treatment and increased mortality. We report a rare case of a 36-year-old man, who underwent open reduction and internal fixation of the left clavicle after inhaled anesthetics. The patient developed dyspnea, hypotension, unremitting hyperthermia, tachycardia, and elevated serum myoglobin, and finally died of pyemia and disseminated intravascular coagulation. We reviewed the process of disease development, summarized the steps of diagnosis, and improved genetic testing. Exome sequencing revealed a new mutation c.8519G>A (p.arg2840 GLN) in the RYR1 gene that could be associated with MH. The gene mutation was also found in his daughter’s genetic test. This case emphasized the importance of the awareness of MH and its atypical clinical symptoms. The presence of dyspnea, hypotension, unremitting hyperthermia, tachycardia, and raised myoglobin in serum might further strengthen the clinical diagnosis of suspected MH.

During the installation of a cable oil terminal, it is easy to leave scratches on the main insulation owing to uneven forces when removing the semi-conductive layer. Scratch defects cause field intensity distortion, which leads to partial discharge and insulation failure. This study attempts to establish a simulation model of a 220 kV cable terminal to determine the effect of the length, depth, and position of the scratch on the maximum field strength at the defect. The simulation and experiment demonstrate that the maximum field strength at the defect increases with greater length and decreases as the depth increases. Therefore, a prediction method for the terminal defect field strength based on a multivariate nonlinear regression model was proposed in this study. When the defect is located at 20mmfromthe root of the stress cone, the maximum field strength is 14.5 MV/ m when the length and depth are 2mmand 1mm, respectively. The maximum field strength at the defect was predicted based on the length, depth, and position of the scratch defect to evaluate the severity of the defect. [ABSTRACT FROM AUTHOR]

Background: Recent studies have established a correlation between folate levels and the incidence of cervical cancer. Given that Human Papillomavirus (HPV) infection is a primary etiological factor in the development of cervical cancer, the nature of the relationship between dietary folate intake and HPV infection remains an area of ongoing investigation. Methods: To investigate the association between dietary folate intake and HPV infection, this study utilized data from the National Health and Nutrition Examination Survey (NHANES) spanning from 2005 to 2016. Multivariate logistic regression analysis was employed to examine the potential associations. Furthermore, the use of restricted cubic splines (RCS) facilitated the exploration of any non-linear correlations. Additionally, subgroup analyses were used to explore this correlation in different populations. Results: The study encompassed a total of 6747 women aged between 18 and 59 years. For every one mcg increase in folate intake, the incidence of HPV infection is reduced by 1% (OR = 0.99, p<0.05). Besides, folate intake was categorized into quartiles as follows: Q1 (<211 mcg/day), Q2 (211–311 mcg/day), Q3 (311–448 mcg/day), and Q4 (>448 mcg/day). The adjusted odds ratios (OR) for the different folate levels were as follows: Q2: 0.94 (95% CI: 0.76–1.16), Q3: 0.84 (95% CI: 0.67–1.04), and Q4: 0.63 (95% CI: 0.49–0.81). The RCS analysis confirmed a nonlinear relationship between dietary folate intake and HPV infection risk. Notably, a significant inverse association was observed when dietary folate intake exceeded 193.847 mcg/day. Conclusions: In conclusion, the findings of this study indicate a negative association between dietary folate intake and the risk of HPV infection. This association demonstrates a nonlinear pattern, particularly evident at higher levels of folate consumption. [ABSTRACT FROM AUTHOR]

AIM: To explore the intensity-dependent effects of exercises to alleviate fear memory generalization in post-traumatic stress disorder (PTSD) and the underlying mechanism. METHODS: Male C57 BL/6J mice were randomly divided into the control group, PTSD group, high-intensity exercise (PTSD-High) group, and low-intensity exercise (PTSD-Low) group. PTSD model were created via using a combination of conditioned foot shock(CF) and single-prolonged stress (SPS). The contextual fear test was used to test the mice's ability to discriminate safety situations from fear condition. Immunofluorescence observed and quantified the newborn immature neurons in the DG area of the mice's hippocampus. ELISA was used to determine the secretion level of serum tissue adiponectin. RESULTS: (1) The immobility times for the PTSD-High and Control groups were significantly lower than those of the PTSD group. (2) Immunofluorescence analysis showed that the cell density, dendritic branching points and length of newborn immature neurons were elevated in High and Low groups compared to the PTSD group. (3) Adiponectin levels in the serum of the control and PTSD High group were significantly higher than those in the PTSD group and PTSD-Low group. CONCLUSION: Fear memory establishment in PTSD mice is associated with decreased hippocampal neurogenesis. High-intensity exercise ameliorated fear memory by enhancing adiponectin secretion and promoting hippocampal neurogenesis. High-intensity exercise exerted better improvement of brain functions to PTSD model. [ABSTRACT FROM AUTHOR]

Polymer‐derived ceramics (PDCs) have become promising candidates for electromagnetic wave (EMW) absorption due to their high thermal stability, design flexibility, and versatile functionality. However, the inevitable porous structure formed during pyrolysis greatly limits its mechanical performance. In this work, Ni–Fe–C/HfO2/SiCN ceramic was prepared by simultaneously doping nickel and iron‐containing metal‐organic framework (Ni–Fe–MOF) nanoparticles and hafnium dioxide (HfO2) nanoparticles into the ceramic precursor, thereby improving its intrinsic brittle structure while granting it extraordinary EMW absorption performance. The flexural strength, flexural modulus, and Vickers hardness of Ni–Fe–C/HfO2/SiCN ceramic were 101.06 MPa, 40.19 GPa, and 13.31 GPa, respectively. These values were 1 132%, 749%, and 191% higher than those of the initial SiCN ceramics. In addition, by optimizing the content of Ni–Fe–MOF, the Ni–Fe–C/HfO2/SiCN ceramic demonstrated satisfying EMW absorption performance from 25°C to 500°C. At 25°C, the minimum reflection loss (RLmin) and effective absorption bandwidth (EAB) were −54.07 dB and 3.95 GHz, covering 94% of the X‐band at a thickness of 2.27 mm. At 500°C, the RLmin and EAB were −33.72 dB and 1.82 GHz, still covering 43% of the X‐band at a thickness of only 1.65 mm. This work provides valuable insight into the integration of mechanical and EMW absorbing functions of PDC materials. [ABSTRACT FROM AUTHOR]