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Abstract Microbial cell factories serve as pivotal platforms for the production of high-value natural products, which tend to accumulate on the cell membrane due to their hydrophobic properties. However, the limited space of the cell membrane presents a bottleneck for the accumulation of these products. To enhance the production of intracellular natural products and alleviate the burden on the cell membrane caused by product accumulation, researchers have implemented various membrane engineering strategies. These strategies involve modifying the membrane components and structures of microbial cell factories to achieve efficient accumulation of target products. This review summarizes recent advances in the application of membrane engineering technologies in microbial cell factories, providing case studies involving Escherichia coli and yeast. Through these strategies, researchers have not only improved the tolerance of cells but also optimized intracellular storage space, significantly enhancing the production efficiency of natural products. This article aims to provide scientific evidence and references for further enhancing the efficiency of similar cell factories.

Losartan is widely used in the treatment of chronic kidney disease (CKD) and has achieved good clinical efficacy, but its exact mechanism is not clear. We performed high-throughput sequencing (HTS) technology to screen the potential target of losartan in treating CKD. According to the HTS results, we found that the tumor necrosis factor (TNF) signal pathway was enriched. Therefore, we conducted in vivo and in vitro experiments to verify it. We found that TNF signal pathway was activated in both unilateral ureteral obstruction (UUO) rats and human proximal renal tubular epithelial cells (HK-2) treated with transforming growth factor-β1 (TGF-β1), while losartan can significantly inhibit TNF signal pathway as well as the expression of fibrosis related genes (such as COL-1, α-SMA and Vimentin). These data suggest that losartan may ameliorate renal fibrosis through modulating the TNF pathway. Resumen: El Losartán es ampliamente utilizado en el tratamiento de la enfermedad renal crónica (CKD) y ha logrado buenos resultados clínicos, pero su mecanismo exacto aún no está claro. Utilizamos la técnica de secuenciación de alto rendimiento (HTS) para detectar posibles dianas de losartán para el tratamiento de la CKD. Según los resultados de HTS, encontramos un enriquecimiento de la vía de señalización del factor de necrosis tumoral (TNF). Así, realizamos experimentos in vivo e in vitro para verificar esto. Encontramos que, tanto en ratas con obstrucción ureteral unilateral (uuo) como en células epiteliales tubulares renales proximal humanas (HK-2) tratadas con factor de crecimiento transformador β1 (TGF-β1), se activó la vía de señalización del TNF. El losartán inhibe significativamente la expresión de las vías de señalización del TNF y genes relacionados con la fibrosis, como COL-1, α-SMA y vicentin. Estos datos sugieren que el losartán puede mejorar la fibrosis renal regulando la vía del TNF.

Abstract Software-defined networking (SDN), a novel network paradigm, separates the control plane and data plane into different network equipment to realize the flexible control of network traffic. Its excellent programmability and global view present many new opportunities. DDoS detection under the SDN context is an important and challenging research field. Some previous works attempted to collect and analyze statistics related to flows, usually recorded in switches, to address DDoS threats. In contrast, other works applied machine learning-based solutions to identify DDoS and achieved promising results. Generally, most previous works need to periodically request flow rules or packets to obtain flow statistics or features to detect stealthy exceptions. Nevertheless, the request for flow rules is very time-consuming and CPU-consuming; moreover may congest the communication channel between the controller and the switches. Therefore, we present FORT, a lightweight DDoS detection scheme, which spreads the rule-based detection algorithm at edge switches and determines whether to start it by periodically retrieving the ports state. A time-series algorithm, ARIMA, is utilized to determine the port statistics adaptively, and an SVM algorithm is applied to detect whether a DDoS attack does occur. Representative experiments demonstrate that FORT can significantly reduce the controller load and provide a reliable detection accuracy. Referring to the false alarm rate of 1.24% in the comparison scheme, the false alarm rate of this scheme is only 0.039%, which significantly reduces the probability of false alarm. Besides, by introducing the alarm mechanism, this scheme can reduce the load of the southbound channel by more than 60% in the normal state.

Aiming at the problem that the swelling force of the red-bed mudstone is smaller than the theoretical value according to the code, this paper develops a triaxial swelling force device for the swelling rock. By placing the sample first and then restraining it, the deformation gap between the rock and the container’s wall is overcome. Through the experimental research of the triaxial expansion force of expansive soil and red-bed mudstone, the rationality and effectiveness of the triaxial expansion force device are verified. According to the experimental test results of the typical red-bed mudstone samples in Sichuan, the changes of the swelling force of the muddy swelling rock is preliminarily summarized. The research results show that the triaxial expansion force device and its test method can overcome the limitations of the existing method of consolidation instrument test. It is simple to operate, is of good practicability, and can be applied to the triaxial expansion force test of expansive soil and expansive rock. The experimental results of comparison of triaxial expansion force and uniaxial expansion force show that the triaxial expansion force of the red-bed mudstone is mostly in the magnitude of MPa, and the expansion force tested by the consolidation instrument is mostly in the magnitude of kPa. Through the analysis of the test results and the observation of the sample after expansion, it is found that the expansion mechanism of the red-bed mudstone is different from that of the expansive soil. The red-bed mudstone is mainly the surface layer of fresh rock that absorbs water and swells and disintegrates. After the surface layer is denuded, the fresh surface absorbs water again to swell and disintegrate. This is a cyclic expansion process layer by layer.

Steam jet condensation is of great importance to pressure suppression containment and automatic depressurization system in nuclear power plant. In this paper, the condensation processes of sonic steam jet in a quiescent subcooled pool are recorded and analyzed, more precise understanding are got in direct contact condensation. Experiments are conducted at atmospheric pressure, and the steam is injected into the subcooled water pool through a vertical nozzle with the inner diameter of 10 mm, water temperature in the range of 25–60 °C and mass velocity in the range of 320–1080 kg/m2s. Richardson number is calculated based on the conservation of momentum for single water jet and its values are in the range of 0.16–2.67. There is no thermal stratification observed in the water pool. Four condensation regimes are observed, including condensation oscillation, contraction, expansion-contraction and double expansion-contraction shapes. A condensation regime map is present based on steam mass velocity and water temperature. The dimensionless steam plume length increase with the increase of steam mass velocity and water temperature, and its values are in the range of 1.4–9.0. Condensation heat transfer coefficient decreases with the increase of steam mass velocity and water temperature, and its values are in the range of 1.44–3.65 MW/m2°C. New more accurate semi-empirical correlations for prediction of the dimensionless steam plume length and condensation heat transfer coefficient are proposed respectively. The discrepancy of predicted plume length is within ± 10% for present experimental results and ± 25% for previous researchers. The discrepancy of predicted condensation heat transfer coefficient is with ± 12%. Keywords: Direct contact condensation, Sonic jet, Condensation regimes, Condensation heat transfer coefficients

Introduction: Synthetic fibrous membranes unveil a promising field in anti-adhesion of tendons. Meanwhile, oriented nanofiber structures have been widely studied and used in the application of biomedical engineering, particularly in repairing and strengthening effects. Methods: In this study, a bi-layer poly(L-lactic acid) (PLLA) electrospun membrane was fabricated, in which the inner oriented fibrous layer was designed to promote tendon healing while outer random aligned layer was designed to prevent peritendinous adhesion. Results: It was found that fibroblasts were aligned along the oriented fiber of membranes in vitro and in a Leghorn chicken model. In biomechanical tests of repaired tendons, no significant difference was found between oriented fibrous membrane and blank control in maximum tensile strength; both oriented fibrous membranes and random fibrous membranes showed lower work of flexion than blank control, which was consistent with gross assessment. Conclusion: It was practicable to promote tendon healing while preventing adhesion via bi-layer PLLA membranes with an inner-oriented-fiber fabricated structure.

This paper reports the histological observation of larvae of Zethenia rufescentaria Motsch. after infection by ZrNPV. Histopathologic study revealed that ZrNPV were multiplied within the nuclear of fat body, epidermis cell, midgut cell, tracheal matrix and blood cell. These cells showed obvious cytopathic effects. The nucleus of infected cells underwent swelled. Under electron microscope, virus and polyhedral of ZrNPV were clearly observed in these nucleus of infected cells. The nucleus of susceptible tissues were fulfilled with polyhedra after 70–140 h. [ABSTRACT FROM AUTHOR]