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Despite their emerging use, the in vivo behaviour of dual-mobility (DM) total hip replacements (THRs) is not well understood. Therefore, the purpose of this study was to assess the articulating surfaces of 20 early-retrieved DM polyethylene liners (mean length of implantation 20.0 ± 18.8 months) for damage to improve the current understanding of their in vivo functional mechanisms. The internal and external surfaces of each liner were visually and geometrically assessed, and the material composition of embedded debris particles were further characterized. Scratching and pitting were the most common modes of damage identified on either surface, and a high incidence of burnishing (50%) and embedded debris (65%) were observed on the internal and external surfaces, respectively. Embedded debris particles were commonly titanium- or iron-based, although other materials such as cobalt-chrome and tantalum were also identified. The geometric assessment demonstrated highly variable damage patterns across the liners, with the internal surfaces commonly presenting with crescent-shaped, circumferential, or circular regions of penetration whilst the external surfaces commonly presented with regions of deep pitting or gouging. This study demonstrates that DM-THRs primarily articulate at the head/liner junction, and that polyethylene liners are capable of rotating about the femoral neck axis, although the extent of this may be limited in some cases. Additionally, this study suggests that intra-prosthetic dislocation and edge loading may remain pertinent failure mechanisms of DM implants despite the advent of highly crosslinked polyethylene and design features, thus highlighting the need for enhanced monitoring of these devices.

Prior to the COVID-19 pandemic (hereafter, ‘the pandemic’), there was already widespread concern about the adequacy of health care resources across Canada. The COVID-19 pandemic exacerbated these concerns exponentially, widening already significant cracks in provincial health care systems. Currently the system is struggling with the exacerbation of wait times for surgeries previously delayed by mandated closures during the pandemic. In Ontario, the backlog of surgeries, and associated backlogs in radiology and other services critical to paediatric care, led to the creation of a consortium of paediatric hospitals committed to advocacy for more funding for paediatrics. Thus far, the provincial and federal governments have agreed to a one-time cash infusion, but the consortium is calling for a permanent increase in funding for paediatrics. A challenge is that the adult sector has also suffered from delays and backlogs. Furthermore, as already noted, older adults have borne the brunt of morbidity and mortality associated with COVID-19. The challenge for the paediatric sector is whether and how to defend the prioritization of children and youth. In this paper, we review four approaches to just allocation – utilitarian ageism, fair innings, the prudential lifespan approach, and prioritarian ageism – and examine their strengths and weaknesses. We conclude by endorsing prioritarian ageism (prioritarianism). Prioritarianism retains the strengths of utilitarian ageism and fair innings while avoiding their weaknesses. Furthermore, because prioritarianism does not treat age as an independent moral criterion, allocation schemes based on this foundation are less susceptible to legal challenge and may be more palatable to the general public.

IntroductionThe food-energy-water (FEW) nexus highlights the interdependencies between the systems that people rely on for these essential resources. For example, globally, over two thirds of freshwater withdrawals are used to produce food, and another 10% is used during energy generation. In addition, the food system uses one eighth of global net energy. Seafood is a nutritionally important food, and it is critical to use freshwater and energy resources efficiently throughout seafood supply chains to safeguard future supplies and to reduce environmental impacts. Diverse seafood production methods result in highly variable resource use across supply chains, which may contribute to siloed efforts within supply chains to improve efficiency, instead of larger efforts that involve multiple seafood supply chains. Additionally, efforts to develop and implement efficiency strategies must be informed by fishers, aquaculturists, processors, and other seafood supply chain actors to avoid investing time and resources into strategies that will have low uptake. A significant proportion of seafood is imported into the U.S., so engaging with industry and stakeholders in the U.S. and abroad is critical for understanding and improving the FEW nexus associated with seafood consumed by Americans.MethodsTo understand how resources are being used, current and potential strategies to improve resource use, and relevant motivations and barriers, we conducted 47 semi-structured interviews from 2019 to 2021 with seafood supply chain actors, including producers and processors. Seafood supply chains included were farmed catfish produced in the U.S., farmed pangasius and shrimp produced in Vietnam, farmed Atlantic salmon produced in Norway, and wild-caught sockeye and pink salmon caught in the U.S.ResultsWe provide detailed descriptions of stages within each supply chain regarding resource use and efficiency strategies, and report higher-level findings that apply across supply chains. There was variation across settings regarding how resources are used and opportunities and barriers for improving efficiencies, but we also found commonalities in settings, indicating that resource-saving strategies or innovations could lead to increased efficiency across multiple supply chains. Interviewees shared that cost savings drove past adoption of, and high interest in, energy conservation practices. Generally, direct costs did not motivate reduced use of freshwater, but associated costs like energy to run pumps and supplies to treat contaminated surface water drove interest in reducing water use.DiscussionEfforts to improve resource use in the U.S. seafood supply should focus on identifying and scaling-up strategies that (i) involve improved efficiency of more than one resource and/or (ii) apply across multiple settings. This work should involve partnerships between industry, government agencies, and academic researchers, and should be informed by supply chain actors’ experiences and insights. The qualitative insights from this study encompass rich descriptions of FEW-relevant factors at the level of specific supply chain stages as well as findings across six major seafood supply chains in three countries. The study provides an essential complement to existing quantitative characterizations of resource use, and enables nuanced and informed responses to challenges.

Background Data on right ventricular (RV) exercise adaptation following acute intermediate and high-risk pulmonary embolism (PE) remain limited. This study aimed to evaluate the symptom burden, RV functional recovery during exercise and cardiopulmonary exercise parameters in survivors of intermediate and high-risk acute PE.Methods We prospectively recruited patients following acute intermediate and high-risk PE at four sites in Australia and UK. Study assessments included stress echocardiography, cardiopulmonary exercise testing (CPET) and ventilation–perfusion (VQ) scan at 3 months follow-up.Results Thirty patients were recruited and 24 (median age: 55 years, IQR: 22) completed follow-up. Reduced peak oxygen consumption (VO2) and workload was seen in 75.0% (n=18), with a persistent high symptom burden (mean PEmb-QoL Questionnaire 48.4±21.5 and emPHasis-10 score 22.4±8.8) reported at follow-up. All had improvement in RV-focused resting echocardiographic parameters. RV systolic dysfunction and RV to pulmonary artery (PA) uncoupling assessed by stress echocardiography was seen in 29.2% (n=7) patients and associated with increased ventilatory inefficiency (V̇E/V̇CO2 slope 47.6 vs 32.4, p=0.03), peak exercise oxygen desaturation (93.2% vs 98.4%, p=0.01) and reduced peak oxygen pulse (p=0.036) compared with controls. Five out of seven patients with RV–PA uncoupling demonstrated persistent bilateral perfusion defects on VQ scintigraphy consistent with chronic thromboembolic pulmonary vascular disease.Conclusion In our cohort, impaired RV adaptation on exercise was seen in almost one-third of patients. Combined stress echocardiography and CPET may enable more accurate phenotyping of patients with persistent symptoms following acute PE to allow timely detection of long-term complications.

Abstract Mycobacterium marinum is a ubiquitous water‐borne non‐tuberculous mycobacterial (NTM) pathogen. In humans, M. marinum infections are acquired through direct inoculation of skin wounds and are almost exclusively localized to skin and soft tissues. Pulmonary infection with M. marinum is extremely rare, and to our knowledge, invasive endobronchial disease has not been reported. Here, we present a case of a 71‐year‐old immunocompetent male surfer with invasive endotracheal M. marinum granulomatous disease. The patient was successfully cured with a regimen of azithromycin 250 mg daily, ethambutol 900 mg (15 mg/kg) daily and rifampicin 600 mg daily for 12 months following culture conversion. This case highlights several important concepts: Firstly, M. marinum infection, including invasive endobronchial infection, should be considered a rare cause of NTM pulmonary disease. Secondly, endotracheal infection can be successfully eradicated with this selected therapeutic regimen. Finally, the absence of M. marinum skin or soft‐tissue infection in this patient, raises the possibility that human disease might also be acquired via inhalation of M. marinum contaminated water in rare circumstances.

The online censorship and subsequent arrest of scholar-activist Walden Bello is the latest instance of a disconcerting trend during a period of hegemonic crisis. To understand how a respected scholar ended up in jail and in grave legal trouble on very feeble accounts, we have to unpack the full implications of this case, and place it in relation to ongoing structural changes within the world-system—namely, the decline of the United States as global hegemon, the ascendancy of far-right authoritarianism as a popular political framework, and the use of institutions and technologies developed under liberal-democratic rule by authoritarian regimes for purposes of social control during a period of flux. The crisis offers an opportunity to reconfigure systemic arrangements through coordinated solidarity networks characterized by forms of organization and ways of relating that embody prerogatives and values different from those that predominate in the modern world-system and from those that reproduce the capitalist world-economy; which, more likely than not, will have authoritarian tendencies in the decades to come. As a conclusion, we offer some of the possibilities the global left has for these upcoming decades in regards of large coalitions aimed at changing the structure of the world-system at large.

Marcuse suggested the alienation of art from society intrinsic to the aesthetic form represents and recollects an unreal world capable of indicting existing social arrangements while simultaneously providing a sensuous experience of another possible, liberated reality denied by established institutions. Drawing on and recasting part of Marcuse’s theory of art and the aesthetic dimension, the author puts forward several theses regarding art, alienation and abolition of the prison-industrial complex (PIC). First, art implies alienation; yet, because of that condition, art offers an antidote to estrangement conducive to PIC abolition. Second, Marx explained why key components of capitalism engender alienation, but expounding upon his analysis reveals how incarceration and the concomitant punitive paradigm also reinforce and reproduce estrangement; art, understood as recollection, can aid in abolition of those institutions. Third, authentic justice implies unimpeded use of creative capacities denied and distorted by the PIC, but art can act as recuperation of those capacities in ways that transform our understanding and practice of justice. Fourth, art can offer a foretaste of liberation while anticipating, expressing and heightening our understanding of the possibilities for fuller freedom and PIC abolition, going against and beyond reformist reforms that have historically functioned to strengthen and expand the PIC. Finally, art augments the spirit of abolition and intimates a mode of being antithetical to imprisonment, the PIC and the alienated way of life associated with criminalization and State coercion.

Extracellular vesicles are mediators of intercellular communication with critical roles in cellular senescence and ageing. In arthritis, senescence is linked to the activation of a pro-inflammatory phenotype contributing to chronic arthritis pathogenesis. We hypothesised that senescent osteoarthritic synovial fibroblasts induce senescence and a pro-inflammatory phenotype in non-senescent osteoarthritic fibroblasts, mediated through extracellular vesicle cargo. Small RNA-sequencing and mass spectrometry proteomics were performed on extracellular vesicles isolated from the secretome of non-senescent and irradiation-induced senescent synovial fibroblasts. β-galactosidase staining confirmed senescence in SFs. RNA sequencing identified 17 differentially expressed miRNAs, 11 lncRNAs, 14 tRNAs and one snoRNA and, 21 differentially abundant proteins were identified by mass spectrometry. Bioinformatics analysis of miRNAs identified fibrosis, cell proliferation, autophagy, and cell cycle as significant pathways, tRNA analysis was enriched for signaling pathways including FGF, PI3K/AKT and MAPK, whilst protein analysis identified PAX3-FOXO1, MYC and TFGB1 as enriched upstream regulators involved in senescence and cell cycle arrest. Finally, treatment of non-senescent synovial fibroblasts with senescent extracellular vesicles confirmed the bystander effect, inducing senescence in non-senescent cells potentially through down regulation of NF-κβ and cAMP response element signaling pathways thus supporting our hypothesis. Understanding the exact composition of EV-derived small RNAs of senescent cells in this way will inform our understanding of their roles in inflammation, intercellular communication, and as active molecules in the senescence bystander effect.

Precise segmentation of wheat spikes from a complex background is necessary for obtaining image-based phenotypic information of wheat traits such as yield estimation and spike morphology. A new instance segmentation method based on a Hybrid Task Cascade model was proposed to solve the wheat spike detection problem with improved detection results. In this study, wheat images were collected from fields where the environment varied both spatially and temporally. Res2Net50 was adopted as a backbone network, combined with multi-scale training, deformable convolutional networks, and Generic ROI Extractor for rich feature learning. The proposed methods were trained and validated, and the average precision (AP) obtained for the bounding box and mask was 0.904 and 0.907, respectively, and the accuracy for wheat spike counting was 99.29%. Comprehensive empirical analyses revealed that our method (Wheat-Net) performed well on challenging field-based datasets with mixed qualities, particularly those with various backgrounds and wheat spike adjacence/occlusion. These results provide evidence for dense wheat spike detection capabilities with masking, which is useful for not only wheat yield estimation but also spike morphology assessments.

Background: Most evaluation in Africa is rooted in dominant neoliberal Western approaches. Imported Western evaluation frames may lack multicultural validity and can lead to wrong conclusions and poor development outcomes. They may also reinforce subjugation and cultural hegemony through neo-imperialism and colonisation of the imaginations of those concerned. The Made in Africa Evaluation (MAE) concept has received attention in recent years as a way to address this challenge. As a relatively nascent construct, however, interested scholars and professionals continue to seek to define and operationalise MAE more effectively. Objective: The objective of this study is to provide a working definition of MAE. Methods: We used the Delphi technique to solicit informed views from expert evaluators working in Africa. We interviewed two additional experts to triangulate and test the validity of those findings. We also tested the Delphi derived definition of MAE through the analysis of six illustrative evaluation reports. Finally, we asked the same panel of experts to complete a survey aimed at clarifying next key steps to advance the construct. Results: The results of our efforts to elucidate a concise definition of MAE yielded the following definition: Evaluation that is conducted based on African Evaluation Association (AfrEA) standards, using localised methods or approaches with the aim of aligning all evaluations to the lifestyles and needs of affected African peoples whilst also promoting African values. Conclusion: We posit that this working definition, however tentative, has the potential to influence the practice, study, and teaching of evaluation in Africa.

Abstract Background In oncology clinical development, objective response rate, disease control rate and early tumor size changes are commonly used as efficacy metrics for early decision-making. However, for immunotherapy trials, it is unclear whether these early efficacy metrics are still predictive of long-term clinical benefit such as overall survival. The goal of this paper is to identify appropriate early efficacy metrics predictive of overall survival for immunotherapy trials. Methods Based on several checkpoint blockade based immunotherapy studies in metastatic melanoma, we evaluated the predictive value of early tumor size changes and RECIST-based efficacy metrics at various time points on overall survival. The cut-off values for tumor size changes to predict survival were explored via tree based recursive partitioning and validated by external data. Sensitivity analyses were performed for the cut-offs. Results The continuous tumor size change metric and RECIST-based trichotomized response metric at different landmark time points were found to be statistically significantly associated with overall survival. The predictive values were higher at Week 12 and 18 than those at Week 24. The percentage of tumor size changes appeared to have comparable or lower predictive values than the RECIST-based trichotomized metric, and a cut-off of approximately 10% tumor reduction appeared to be reasonable for predicting survival. Conclusions An approximate 10% tumor reduction may be a reasonable cut-off for early decision-making while the RECIST-based efficacy metric remains the primary tool. Early landmark analysis is especially useful for decision making when accrual is fast. Composite response rate (utilizing different weights for PR/CR and SD) may be worth further investigation. Trial registration Clinical trials gov, NCT01295827, Registered February 15, 2011; NCT01704287, Registered October 11, 2012; NCT01866319, Registered May 31, 2013.

The observed movements of humans and animals are realizations of complex spatiotemporal processes. Recent advances in location-aware technologies have rendered trajectory data ubiquitous. Examining the sequenced, instantaneous locations found in movement trajectory data for information reconstructing the location or state of the mover between observed points comprises a primary focus in Time Geography and related disciplines. The PySTPrism toolbox introduced in this paper provides a straightforward and open-source implementation of the Probabilistic Space Time Prism, in addition to related tools from Time Geography. PySTPrism is implemented in Python using the ArcPy module in ArcGIS Pro Desktop.

Aquaculture is growing in Florida as it is worldwide, but in Florida aquaculture has not yet reached its substantial potential. To understand why this might be, the University of Florida's Institute of Sustainable Food Systems organized and convened a meeting between small-agency, industry, and academic stakeholders to discuss the opportunities and obstacles to aquaculture in Florida. This 6-page fact sheet written by Edward Camp, Taryn Garlock, and James Anderson and published by the UF/IFAS School of Forest Resources and Conservation, Program in Fisheries and Aquatic Sciences, describes the proceedings of this meeting in the context of the broader scientific literature explaining why aquaculture ventures do and sometimes do not succeed.

Perennial grains could simultaneously provide food for humans and a host of ecosystem services, including reduced erosion, minimized nitrate leaching, and increased carbon capture. Yet most of the world’s food and feed is supplied by annual grains. Efforts to domesticate intermediate wheatgrass (Thinopyrumn intermedium, IWG) as a perennial grain crop have been ongoing since the 1980’s. Currently, there are several breeding programs within North America and Europe working toward developing IWG into a viable crop. As new breeding efforts are established to provide a widely adapted crop, questions of how genomic and phenotypic data can be used among sites and breeding programs have emerged. Utilizing five cycles of breeding data that span 8 years and two breeding programs, University of Minnesota, St. Paul, MN, and The Land Institute, Salina, KS, we developed genomic selection (GS) models to predict IWG traits. Seven traits were evaluated with free-threshing seed, seed mass, and non-shattering being considered domestication traits while agronomic traits included spike yield, spikelets per inflorescence, plant height, and spike length. We used 6,199 genets – unique, heterozygous, individual plants – that had been profiled with genotyping-by-sequencing, resulting in 23,495 SNP markers to develop GS models. Within cycles, the predictive ability of GS was high, ranging from 0.11 to 0.97. Across-cycle predictions were generally much lower, ranging from −0.22 to 0.76. The prediction ability for domestication traits was higher than agronomic traits, with non-shattering and free threshing prediction abilities ranging from 0.27 to 0.75 whereas spike yield had prediction abilities ranging from −0.22 to 0.26. These results suggest that progress to reduce shattering and increase the percent free-threshing grain can be made irrespective of the location and breeding program. While site-specific programs may be required for agronomic traits, synergies can be achieved in rapidly improving key domestication traits for IWG. As other species are targeted for domestication, these results will aid in rapidly domesticating new crops.

Abstract Background Methotrexate (MTX) remains the disease-modifying anti-rheumatic drug of first choice in rheumatoid arthritis (RA) but response varies. Predicting non-response to MTX could enable earlier access to alternative or additional medications and control of disease progression. We aimed to identify baseline predictors of non-response to MTX and combine these into a prediction algorithm. Methods This study included patients recruited to the Rheumatoid Arthritis Medication Study (RAMS), a UK multi-centre prospective observational study of patients with RA or undifferentiated polyarthritis, commencing MTX for the first time. Non-response to MTX at 6 months was defined as “no response” using the European League Against Rheumatism (EULAR) response criteria, discontinuation of MTX due to inefficacy or starting biologic therapy. The association of baseline demographic, clinical and psychosocial predictors with non-response was assessed using logistic regression. Predictive performance was assessed using the area under the receiver operating characteristic curve (AUC) and calibration plots. Results Of 1050 patients, 449 (43%) were classified as non-responders. Independent multivariable predictors of MTX non-response (OR (95% CI)) were rheumatoid factor (RF) negativity (0.62 (0.45, 0.86) for RF positivity versus negativity), higher Health Assessment Questionnaire score (1.64 (1.25, 2.15)), higher tender joint count (1.06 (1.02, 1.10)), lower Disease Activity score in 28 joints (0.29 (0.23, 0.39)) and higher Hospital Anxiety and Depression Scale anxiety score (1.07 (1.03, 1.12)). The optimism-corrected AUC was 0.74. Conclusions This is the first model for MTX non-response to be developed in a large contemporary study of patients commencing MTX in which demographic, clinical and psychosocial predictors were considered. Patient anxiety was a predictor of non-response and could be addressed at treatment commencement.

This article draws from John Dewey’s philosophy of education, ideas about democracy and pragmatist assumptions to explain how his articles for The New Republic functioned pedagogically. Taking media as a mode of public pedagogy, and drawing extensively from Dewey’s Democracy and Education, as well as from his book The Public and its Problems, the article explores the relationships between communication, education and democracy using the expanded conceptions of all the aforementioned advanced by Dewey. Borrowing insights from Randolph Bourne, who used Dewey’s own ideas to criticize his mentor’s influence on intellectuals who supported US involvement in World War I, the analysis explores the contradictions within Dewey’s public pedagogy. The article suggests Dewey’s relevance as a public intellectual in the liberal-progressive press, his view of the State and some of his related presuppositions produced a tension in his thought, delimiting democratic possibilities while simultaneously pointing toward greater democratic potentials. The essay concludes by suggesting that learning from both Dewey and Bourne prompts us to get beyond the former’s public/private dualism to realize what he called the “Great Community” by communicating and practicing the Commons.

Abstract Background The concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce immune response in the treated individual. However, in elderly patients age-related changes in synthetic activity of the implanted cells and subsequent alterations in tissue protein content may affect therapeutic outcomes. In this study we investigated the effect of donor age on proteome composition of tenocyte-derived tendon tissue-engineered constructs. Results Liquid chromatography tandem mass spectrometry was used to assess the proteome of tissue-engineered constructs derived from young and old equine tenocytes. Ageing was associated with altered extracellular matrix composition, especially accumulation of collagens (type I, III and XIV), and lower cytoskeletal turnover. Proteins involved in cell responsiveness to mechanical stimuli and cell-extracellular matrix interaction (calponin 1, palladin, caldesmon 1, cortactin) were affected. Conclusions This study demonstrated significant changes in proteome of engineered tendon derived from young and old tenocytes, indicating the impact of donor age on composition of autologous constructs.

Abstract Background The conduct of research in settings affected by disasters such as hurricanes, floods and earthquakes is challenging, particularly when infrastructures and resources were already limited pre-disaster. However, since post-disaster research is essential to the improvement of the humanitarian response, it is important that adequate research ethics oversight be available. Methods We aim to answer the following questions: 1) what do research ethics committee (REC) members who have reviewed research protocols to be conducted following disasters in low- and middle-income countries (LMICs) perceive as the key ethical concerns associated with disaster research?, and 2) in what ways do REC members understand these concerns to be distinct from those arising in research conducted in non-crisis situations? This qualitative study was developed using interpretative description methodology; 15 interviews were conducted with REC members. Results Four key ethical issues were identified as presenting distinctive considerations for disaster research to be implemented in LMICs, and were described by participants as familiar research ethics issues that were amplified in these contexts. First, REC members viewed disaster research as having strong social value due to its potential for improving disaster response, but also as requiring a higher level of justification compared to other research settings. Second, they identified vulnerability as an overarching concern for disaster research ethics, and a feature that required careful and critical appraisal when assessing protocols. They noted that research participants’ vulnerabilities frequently change in the aftermath of a disaster and often in unpredictable ways. Third, they identified concerns related to promoting and maintaining safety, confidentiality and data security in insecure or austere environments. Lastly, though REC members endorsed the need and usefulness of community engagement, they noted that there are significant challenges in a disaster setting over and above those typically encountered in global health research to achieve meaningful community engagement. Conclusion Disaster research presents distinctive ethical considerations that require attention to ensure that participants are protected. As RECs review disaster research protocols, they should address these concerns and consider how justification, vulnerability, security and confidentially, and community engagement are shaped by the realities of conducting research in a disaster.

Fusarium Head Blight (FHB) has emerged in spring wheat production in Pacific Northwest during the last decade due to factors including climate changes, crop rotations, and tillage practices. A breeding population with 170 spring wheat lines was established and screened over a 2-year period in multiple locations for FHB incidence (INC), severity (SEV), and deposition of the mycotoxin, deoxynivalenol (DON). A genome-wide association study suggested that the detectable number of genetic loci and effects are limited for marker-assisted selection. In conjunction with the success of breeding on FHB resistance in other programs, genomic selection (GS) was suggested as a better option. To evaluate the prediction accuracy of GS in the current breeding population, we conducted a variety of validations by varying proportions of testing populations and cohorts based on both FHB resistance and market class, including soft white spring (SWS), hard white spring (HWS), and hard red spring (HRS). We found that INC had higher heritability, higher correlation across years and locations, and higher prediction accuracy than SEV and DON. Prediction accuracy varied among the scenarios that restricted the testing population to a certain cohort. For a small set of newly developed or introduced lines (

Many analyses of fishery recovery have demonstrated the potential biological and economic benefits of management reform, but few have compared these to the associated costs of management upgrades, which can be substantial. This study aims to determine if the projected economic benefits of management reform outweigh the increases in management costs required to achieve those benefits. To answer this question, we developed a database of country-level fisheries management costs and use those to estimate the country-level costs of management changes. We use this framework to compare estimates of future costs of management upgrades against their economic benefits in terms of profit. Results indicate that for most nations, including the top 25 fishing nations, management upgrades outweigh their associated costs. This result is robust to a number of alternative assumptions about costs. Results also suggest that stronger reforms such as rights-based management, although sometimes more expensive to implement, can lead to greater net economic benefits compared to alternatives.

Experiments involving mass spectrometry (MS)-based proteomics are widely used for analyses of connective tissues. Common examples include the use of relative quantification to identify differentially expressed peptides and proteins in cartilage and tendon. We are working on characterising so-called ‘neopeptides’, i.e. peptides formed due to native cleavage of proteins, for example under pathological conditions. Unlike peptides typically quantified in MS workflows due to the in vitro use of an enzyme such as trypsin, a neopeptide has at least one terminus that was not due to the use of trypsin in the workflow. The identification of neopeptides within these datasets is important in understanding disease pathology, and the development of antibodies that could be utilised as diagnostic biomarkers for diseases, such as osteoarthritis, and targets for novel treatments. Our previously described neopeptide data analysis workflow was laborious and was not amenable to robust statistical analysis, which reduced confidence in the neopeptides identified. To overcome this, we developed ‘Neopeptide Analyser’, a user friendly neopeptide analysis tool used in conjunction with label-free MS quantification tool Progenesis QIP for proteomics. Neopeptide Analyser filters data sourced from Progenesis QIP output to identify neopeptide sequences, as well as give the residues that are adjacent to the peptide in its corresponding protein sequence. It also produces normalised values for the neopeptide quantification values and uses these to perform statistical tests, which are also included in the output. Neopeptide Analyser is available as a Java application for Mac, Windows and Linux. The analysis features and ease of use encourages data exploration, which could aid the discovery of novel pathways in extracellular matrix degradation, the identification of potential biomarkers and as a tool to investigate matrix turnover. Neopeptide Analyser is available from https://github.com/PGB-LIV/neo-pep-tool/releases/.

Although considered rare, airborne pollen can be deposited far from its place of origin under a confluence of favorable conditions. Temporally anomalous records of Cupressacean pollen collected from January air samples in London, Ontario, Canada have been cited as a new case of long-distance transport. Data on pollination season implicated Juniperus ashei (mountain cedar), with populations in central Texas and south central Oklahoma, as the nearest source of the Cupressacean pollen in the Canadian air samples. This finding is of special significance given the allergenicity of mountain cedar pollen. While microscopy is used extensively to identify particles in the air spora, pollen from all members of the Cupressaceae, including Juniperus, are morphologically indistinguishable. Consequently, we implemented a molecular approach to characterize Juniperus pollen using PCR in order to test the long-distance transport hypothesis. Our PCR results using species-specific primers confirmed that the anomalous Cupressacean pollen collected in Canada was from J. ashei. Forward trajectory analysis from source areas in Texas and the Arbuckle Mountains in Oklahoma and backward trajectory analysis from the destination area near London, Ontario were completed using models implemented in HYSPLIT4 (Hybrid Single-Particle Lagrangian Integrated Trajectory). Results from these trajectory analyses strongly supported the conclusion that the J. ashei pollen detected in Canada had its origins in Texas or Oklahoma. The results from the molecular findings are significant as they provide a new method to confirm the long-distance transport of pollen that bears allergenic importance.

The influence of different types of salts (NaCl, CaCl 2 , MgCl 2 , NaHCO 3 , and Na 2 SO 4 ) on the surface characteristics of unconditioned calcite and dolomite particles, and conditioned with stearic acid, was investigated. This study used zeta potential measurements to gain fundamental understanding of physico-chemical mechanisms involved in surface charge modification of carbonate minerals in the presence of diluted salt solutions. By increasing the salt concentration of divalent cationic salt solution (CaCl 2 and MgCl 2 ), the zeta potential of calcite particles was altered, resulting in charge reversal from negative to positive, while dolomite particles maintained positive zeta potential. This is due to the adsorption of potential-determining cations (Ca 2 + and Mg 2 + ), and consequent changes in the structure of the diffuse layer, predominantly driven by coulombic interactions. On the other hand, chemical adsorption of potential-determining anions (HCO 3 − and SO 4 2 − ) maintained the negative zeta potential of carbonate surfaces and increased its magnitude up to 10 mM, before decreasing at higher salt concentrations. Physisorption of stearic acid molecules on the calcite and dolomite surfaces changed the zeta potential to more negative values in all solutions. It is argued that divalent cations (Ca 2 + and Mg 2 + ) would result in positive and neutral complexes with stearic acid molecules, which may result in strongly bound stearic acid films, whereas ions resulting in negative mineral surface charges (SO 4 2 − and HCO 3 − ) will cause stearic acid films to be loosely bound to the carbonate mineral surfaces. The suggested mechanism for surface charge modification of carbonates, in the presence of different ions, is changes in both distribution of ions in the diffuse layer and its structure as a result of ion adsorption to the crystal lattice by having a positive contribution to the disjoining pressures when changing electrolyte concentration. This work extends the current knowledge base for dynamic water injection design by determining the effect of salt concentration on surface electrostatics.

Deoxynivalenol (DON) is a mycotoxin affecting wheat quality. The formation of the “masked” mycotoxin deoxinyvalenol-3-glucoside (D3G) results from a defense mechanism the plant uses for detoxification. Both mycotoxins are important from a food safety point of view. The aim of this work was to analyze DON and D3G content in inoculated near-isogenic wheat lines grown at two locations in Minnesota, USA during three different years. Regression analysis showed positive correlation between DON content measured with LC and GC among wheat lines, locality and year. The relationship between DON and D3G showed a linear increase until a certain point, after which the DON content and the D3G increased. Wheat lines having higher susceptibility to Fusarium showed the opposite trend. ANOVA demonstrated that the line and location have a greater effect on variation of DON and D3G than do their interaction among years. The most important factor affecting DON and D3G was the growing location. In conclusion, the year, environmental conditions and location have an effect on the D3G/DON ratio in response to Fusarium infection.

This Special Issue of Cosmopolitan Civil Societies Journal focuses on the domination of social and political relations by Ethnocracy – rule or would-be rule by an ethnic group or ethnos, as distinct from Democracy or rule by the demos of all the people. Ethnocracy encompasses state regimes and associated political movements and parties that discriminate systematically in favour of a particular ethnic group (or groups) and against others. When we proposed the Special Issue in late 2014 ethnocratic practices were as prevalent as they had ever been; and now two years later they appear to be on the increase with an ethno-populist upsurge and the election or threatened election of governments pursuing ethnocratic agendas. From India to the USA, from Russia to Hungary, leading politicians openly discriminate against ethnic ‘others’ to attract support from ‘their own’ ethnic groups; across the European Union and in other liberal democracies they increasingly scapegoat ‘immigrants’ to hide their own inadequacies and further their political objectives. Now, more than ever, it is critical that the dynamics of ethnocracy are more clearly understood. This Issue documents the logics of ethnocracy in a variety of different contexts, posing questions about how it develops and how it can be challenged.

Ethnocracy means ‘government or rule by a particular ethnic group’ or ethnos, specified by language, religion, ‘race’ and/or other components . It has been developed from a general imprecise label into an analytical concept sometimes contrasted with democracy or rule by the demos, the people in general. Primarily it was developed as national ethnocracy for regimes in contemporary national states which claim to be ‘democratic’, and it was mainy pioneered by the Israeli geographer Oren Yiftachel to analyse ethnically-biased policies and the asymmetrical power relations of Israeli Jews and Palestinians. However, it can be extended to several other contexts each of which has its own particular dynamics. Yiftachel himself extended it ‘down’ to city level and specifically urban ethnocracy; and we can further explore how cities and city government can moderate state ethnocracy. But going beyond the national and the urban, and the particularities of the Israeli case, the concept can be enriched in other ways, and I suggest three further extensions: firstly, ‘back’ to imperial ethnocracy which often preceded and gave birth to national ethnocracy; secondly, and more speculatively, it can be extended ‘forwards’ to the (usually mis-named) ‘post-conflict’ or power-sharing stages of ‘peace processes’, to what we might call shared or ‘post-conflict’ ethnocracy; and thirdly, it can perhaps be extended to contemporary religious-political conflicts which are at least partly transnational in character, to what could be called religious or ‘post-national’ ethnocracy. The five variants of ethnocracy and their varied inter-relations can help tie together different features of ethno-national conflicts. However questions remain: about, for instance, the variable and relative importance of ethnicity’s different components; about where to draw the boundary between ethnocracy and democracy; and about possibly rival concepts such as ‘ethnic democracy’ on one side and ‘apartheid’ on the other. Keywords: national, urban, imperial, ‘post-conflict’ and ‘post-national’ ethnocracies; democracy; majoritarianism

Research conducted following natural disasters such as earthquakes, floods or hurricanes is crucial for improving relief interventions. Such research, however, poses ethical, methodological and logistical challenges for researchers. Oversight of disaster research also poses challenges for research ethics committees (RECs), in part due to the rapid turnaround needed to initiate research after a disaster. Currently, there is limited knowledge available about how RECs respond to and appraise disaster research. To address this knowledge gap, we investigated the experiences of REC members who had reviewed disaster research conducted in low- or middle-income countries.We used interpretive description methodology and conducted in-depth interviews with 15 respondents. Respondents were chairs, members, advisors, or coordinators from 13 RECs, including RECs affiliated with universities, governments, international organizations, a for-profit REC, and an ad hoc committee established during a disaster. Interviews were analyzed inductively using constant comparative techniques.Through this process, three elements were identified as characterizing effective and high-quality review: timeliness, responsiveness and rigorousness. To ensure timeliness, many RECs rely on adaptations of review procedures for urgent protocols. Respondents emphasized that responsive review requires awareness of and sensitivity to the particularities of disaster settings and disaster research. Rigorous review was linked with providing careful assessment of ethical considerations related to the research, as well as ensuring independence of the review process.Both the frequency of disasters and the conduct of disaster research are on the rise. Ensuring effective and high quality review of disaster research is crucial, yet challenges, including time pressures for urgent protocols, exist for achieving this goal. Adapting standard REC procedures may be necessary. However, steps should be taken to ensure that ethics review of disaster research remains diligent and thorough.

Neurocysticercosis (NCC) is a parasitic infection of the central nervous system caused by Taenia solium larval cysts. Its epidemiology in cysticercosis-nonendemic regions is poorly understood, and the role of public health institutions is unclear. To determine the incidence of NCC and to pilot screening of household contacts for tapeworms, we conducted population-based active surveillance in Oregon. We screened for T. solium infection by examining hospital billing codes and medical charts for NCC diagnosed during January 1, 2006–December 31, 2009 and collecting fecal and blood samples from household contacts of recent case-patients. We identified 87 case-patients, for an annual incidence of 0.5 cases per 100,000 general population and 5.8 cases per 100,000 Hispanics. In 22 households, we confirmed 2 additional NCC case-patients but no current adult intestinal tapeworm infections. NCC is of clinical and public health concern in Oregon, particularly among Hispanics. Public health intervention should focus on family members because household investigations can identify additional case-patients.

We conducted a case–control study to describe the clinical and epidemiologic characteristics of an outbreak of pandemic (H1N1) 2009 at a Canadian military cadet training center. We found that asthma and obesity confer greater risk for infection. Viral shedding was detected by PCR up to 18 days after symptom onset.

This paper documents the development of nocturnal flows in the wide open Phoenix, Arizona (U.S.A) valley (30 km × 100 km) that is bordered by a large nearly flat plain to the west and high mountains to the north and east. Local thermally driven winds concomitant with the absence of significant synoptic pressure gradients dominate typical winter conditions in the Phoenix valley. The purpose of the Phoenix Air Flow Experiment (PAFEX-1) was to study the development of thermally driven flows during the evening transition in a sloping valley and describe the general pattern of transport and dispersion of contaminants during transition periods and at night. Measurements were made using a tethered balloon, sonic anemometer, balloon-based aerosol sampler, radiation sensors, cup anemometers, thermistors and humidity sensors in conjunction with data collected from 44 standard meteorological stations located throughout the valley. Over the period of 15 days in January and February 1998 the general diurnal flow patterns were repeatable, but varied substantially around the valley. This paper focuses on a case study of the evening transition, nocturnal circulation and morning breakdown of the nocturnal circulation on the night of 31 January and morning of 1 February. Central valley measurements were consistent with the notion that the evening transition is associated with a moving front, followed by intense mixing and the movement of the front to establish down-valley winds. Flows originating from different slopes led to the arrival of fronts at the various measurement locations at different times. These flows intrude into the valley and interact with each other, often causing multi-layered vertical structure. The intrusions respond to the evolving stratification and cause striking variability of these layers, for example, periodic wind and temperature disturbances corresponding to the arrival of new intrusive fronts. The evolution of the boundary layer was found to have a direct bearing on the pollution concentrations in the Phoenix air shed.

Wilms' tumors, or nephroblastomas, are thought to arise from abnormal postnatal retention and dysregulated differentiation of nephrogenic progenitor cells that originate as a condensed metanephric mesenchyme within embryonic kidneys. We have previously shown that the transcriptional regulator CITED1 (CBP/p300-interacting transactivators with glutamic acid [E]/aspartic acid [D]-rich C-terminal domain) is expressed exclusively in these nephrogenic progenitor cells and is downregulated as they differentiate to form nephronic epithelia. In the current study, we show that CITED1 expression persists in blastemal cell populations of both experimental rat nephroblastomas and human Wilms' tumors, and that primary human Wilms' tumors presenting with disseminated disease show the highest level of CITED1 expression. Unlike the predominantly cytoplasmic subcellular localization of CITED1 in the normal developing kidney, CITED1 is clearly detectable in the nuclear compartment of Wilms' tumor blastema. These findings indicate that CITED1 is a marker of primitive blastema in Wilms' tumors and suggest that persistent expression and/or altered subcellular localization of CITED1 in the condensed metanephric mesenchyme could play a role in Wilms' tumor initiation and pathogenesis.

ABSTRACT The synthesis of peptidoglycan (PG) in bacteria is a crucial process controlling cell shape and vitality. In contrast to bacteria such as Escherichia coli that grow by dispersed lateral insertion of PG, little is known of the processes that direct polar PG synthesis in other bacteria such as the Rhizobiales. To better understand polar growth in the Rhizobiales Agrobacterium tumefaciens, we first surveyed its genome to identify homologs of (~70) well-known PG synthesis components. Since most of the canonical cell elongation components are absent from A. tumefaciens, we made fluorescent protein fusions to other putative PG synthesis components to assay their subcellular localization patterns. The cell division scaffolds FtsZ and FtsA, PBP1a, and a Rhizobiales- and Rhodobacterales-specific l,d-transpeptidase (LDT) all associate with the elongating cell pole. All four proteins also localize to the septum during cell division. Examination of the dimensions of growing cells revealed that new cell compartments gradually increase in width as they grow in length. This increase in cell width is coincident with an expanded region of LDT-mediated PG synthesis activity, as measured directly through incorporation of exogenous d-amino acids. Thus, unipolar growth in the Rhizobiales is surprisingly dynamic and represents a significant departure from the canonical growth mechanism of E. coli and other well-studied bacilli. IMPORTANCE Many rod-shaped bacteria, including pathogens such as Brucella and Mycobacteriu, grow by adding new material to their cell poles, and yet the proteins and mechanisms contributing to this process are not yet well defined. The polarly growing plant pathogen Agrobacterium tumefaciens was used as a model bacterium to explore these polar growth mechanisms. The results obtained indicate that polar growth in this organism is facilitated by repurposed cell division components and an otherwise obscure class of alternative peptidoglycan transpeptidases (l,d-transpeptidases). This growth results in dynamically changing cell widths as the poles expand to maturity and contrasts with the tightly regulated cell widths characteristic of canonical rod-shaped growth. Furthermore, the abundance and/or activity of l,d-transpeptidases appears to associate with polar growth strategies, suggesting that these enzymes may serve as attractive targets for specifically inhibiting growth of Rhizobiales, Actinomycetales, and other polarly growing bacterial pathogens.

Neuroimaging studies involving human subjects raise a range of ethics issues. Many of these issues are heightened in the context of neuroimaging research involving persons with mental health disorders. There has been growing interest in these issues among legal scholars, philosophers, social scientists, and as well as neuroimagers over the last decade. Less clear, however, is the extent to which members of the neuroimaging community are engaged with these issues when they undertake their research and report results. In this study, we analyze the peer-reviewed review literature involving fMRI as applied to the study of mental health disorders. Our hypothesis is that, due to the critical orientation of reviews, and the vulnerability of mental health population, the penetrance of neuroethics will be higher in the review literature in this area than it is in the primary fMRI research literature more generally. We find that while authors of reviews do focus a great deal of attention on the methodological limitations of the studies they discussed, contrary to our hypothesis, they do not frame concerns in ethical terms despite their ethical significance. We argue that an ethics lens on such discussion would increase the knowledge-value of this scholarly work.

Advocates of Invitational Education theory and practice believe consistent application of an ICORT mindset upon an institution's people, places, policies, programs, and processes (5Ps) can optimize and sustain success for all stakeholders. Given a professional learning community (PLC) is impacted by and also influences all of an institution's 5Ps, this reflective essay describes the benefit of applying IE principles prior to implementation of a PLC seeking to sustain student success and school effectiveness.

BACKGROUND While historical declines in human mortality are clearly shaped by lifestyle and environmental improvements, modeling patterns is difficult because intrinsic and extrinsic processes shape mortality through complex stochastic interactions. OBJECTIVE To develop a stochastic model describing intrinsic and extrinsic mortality rates and quantify historical mortality trends in terms of parameters describing the rates. METHODS Based on vitality, a stochastic age-declining measure of survival capacity, extrinsic mortality occurs when an extrinsic challenge exceeds the remaining vitality and intrinsic mortality occurs with the complete loss of vitality by aging. Total mortality depends on the stochastic loss rate of vitality and the magnitude and frequency of extrinsic challenges. Parameters are estimated using maximum likelihood. RESULTS Fitting the model to two centuries of adult Swedish period data, intrinsic mortality dominated in old age and gradually declined over years. Extrinsic mortality increased with age and exhibited step-like decline over years driven by high-magnitude, low-frequency challenges in the 19th century and low-magnitude high-frequency challenges in the 20th century. CONCLUSIONS The Swedish mortality was driven by asynchronous intrinsic and extrinsic processes, coinciding with well-known epidemiological patterns involving lifestyle and health care. Because the processes are largely independent, predicting future mortality requires projecting trends of both processes. COMMENTS The model merges point-of-view and classical hazard rate mortality models and yields insights not available from either model individually. To obtain a closed form the intrinsic-extrinsic interactions were simplified, resulting in biased, but correctable, parameters estimates.

The normal development and maintenance of CNS white matter, and its responses to disease and injury, are defined by synergies between axons, oligodendrocytes, astrocytes and microglia, and further influenced by peripheral components such as the gut microbiome and the endocrine and immune systems. Consequently, mechanistic insights, therapeutic approaches and safety tests rely ultimately on in vivo models and clinical trials. However, in vitro models that replicate the cellular complexity of the CNS can inform these approaches, reducing costs and minimising the use of human material or experimental animals; in line with the principles of the 3Rs. Using electrophysiology, pharmacology, time-lapse imaging, and immunological assays, we demonstrate that murine spinal cord-derived myelinating cell cultures recapitulate spinal-like electrical activity and innate CNS immune functions, including responses to disease-relevant myelin debris and pathogen associated molecular patterns (PAMPs). Further, we show they are (i) amenable to siRNA making them suitable for testing gene-silencing strategies; (ii) can be established on microelectrode arrays (MEAs) for electrophysiological studies; and (iii) are compatible with multi-well microplate formats for semi-high throughput screens, maximising information output whilst further reducing animal use. We provide protocols for each of these. Together, these advances increase the utility of this in vitro tool for studying normal and pathological development and function of white matter, and for screening therapeutic molecules or gene targets for diseases such as multiple sclerosis, motor neuron disease or spinal cord injury, whilst avoiding in vivo approaches on experimental animals.

In his introduction to Music and Text, Steven Scher declares that its fourteen essays 'demonstrate how musical and literary studies can combine forces effectively on the common ground of contemporary [...]

Regarding Gerard Thomas's article on gay priests ('A Gay Priest Speaks Out,' January 28): I confess deep conflict about this matter. I believe the church's teaching on homosexuality is misguided [...]

Abstract  A multiprocessor scheduling scheme is presented for supporting hierarchical containers that encapsulate sporadic soft and hard real-time tasks. In this scheme, each container is allocated a specified bandwidth, which it uses to schedule its children (some of which may also be containers). This scheme is novel in that, with only soft real-time tasks, no utilization loss is incurred when provisioning containers, even in arbitrarily deep hierarchies. Presented experiments show that the proposed scheme performs well compared to conventional real-time scheduling techniques that do not provide container isolation. [ABSTRACT FROM AUTHOR]

Abstract  Since its introduction in 1987, Benjamin Freedman’s principle of clinical equipoise has enjoyed widespread uptake in bioethics discourse. Recent years, however, have witnessed a growing consensus that the principle is fundamentally flawed. One of the most vocal critics has undoubtedly been Franklin Miller. In a 2008 paper, Steven Joffe and Miller build on this critical work, offering a new conception of clinical research ethics based on science, taking what they call a “scientific orientation” toward the ethics of clinical research. Though there is much to recommend Joffe and Miller’s scientifically oriented conception of clinical research ethics, I believe that both the critical and constructive projects suffer from the same basic mistake: inattention to context. The internal norms of science cannot be fully specified, let alone satisfied, independently of contextual (external) factors that only come into view when we are attentive to the particular context of that form of inquiry. [ABSTRACT FROM AUTHOR]

Abstract   We consider schemes for enacting task share changes—a process called reweighting—on real-time multiprocessor platforms. Our particular focus is reweighting schemes that are deployed in environments in which tasks may frequently request significant share changes. Prior work has shown that fair scheduling algorithms are capable of reweighting tasks with minimal allocation error and that partitioning-based scheduling algorithms can reweight tasks with better average-case performance, but greater error. However, preemption and migration overheads can be high in fair schemes. In this paper, we consider the question of whether non-fair, earliest-deadline-first ( ) global scheduling techniques can improve the accuracy of reweighting relative to partitioning-based schemes and provide improved average-case performance relative to fair-scheduled systems. Our conclusion is that, for soft real-time systems, global schemes provide a good mix of accuracy and average-case performance. [ABSTRACT FROM AUTHOR]

Abstract   The earliest-pseudo-deadline-first ( ) Pfair scheduling algorithm is less expensive than some other known Pfair algorithms, but is not optimal for scheduling recurrent real-time tasks on more than two processors. In prior work, sufficient per-task weight (i.e., utilization) restrictions were established for ensuring that tasks either do not miss their deadlines or have bounded tardiness when scheduled under . Implicit in these restrictions is the assumption that the total system utilization may equal the total available processing capacity (i.e., the total number of processors). This paper considers an orthogonal issue, namely, determining a sufficient restriction on the total utilization of a task set for it to be schedulable (i.e., a schedulable utilization bound) under , assuming that there are no per-task weight restrictions. We prove that a task set with total utilization at most is correctly scheduled under on M processors, regardless of how large each task’s weight is. At present, we do not know whether this value represents the worst-case for , but we do provide a counterexample that shows that it cannot be improved to exceed 86% of the total processing capacity. The schedulable utilization bound we derive is expressed in terms of the maximum weight of any task, and hence, if this value is known, may be used to schedule task sets with total utilization greater than . [ABSTRACT FROM AUTHOR]

Abstract   There has been much recent interest in the use of the earliest-deadline-first ( ) algorithm for scheduling soft real-time sporadic task systems on identical multiprocessors. In hard real-time systems, a significant disparity exists between -based schemes and Pfair scheduling: on M processors, the worst-case schedulable utilization for all known variants is approximately M/2, whereas it is M for optimal Pfair algorithms. This is unfortunate because -based algorithms entail lower scheduling and task-migration overheads. However, such a disparity in schedulability can be alleviated by easing the requirement that all deadlines be met, which may be sufficient for soft real-time systems. In particular, in recent work, we have shown that if task migrations are not restricted, then (i.e. , global ) can ensure bounded tardiness for a sporadic task system with no restrictions on total utilization. Unrestricted task migrations in global may be unappealing for some systems, but if migrations are forbidden entirely, then bounded tardiness cannot be guaranteed. In this paper, we address the issue of striking a balance between task migrations and system utilization by proposing an algorithm called , which is based upon and treads a middle path, by restricting, but not eliminating, task migrations. Specifically, under , the ability to migrate is required for at most M−1 tasks, and it is sufficient that every such task migrate between two processors and at job boundaries only. , like global , can ensure bounded tardiness to a sporadic task system as long as the available processing capacity is not exceeded, but, unlike global , may require that per-task utilizations be capped. The required cap is quite liberal, hence, should enable a wide range of soft real-time applications to be scheduled with no constraints on total utilization. [ABSTRACT FROM AUTHOR]