Search

Your search keyword '"Jakob, Thilo"' showing total 425 results
Start Over Author "Jakob, Thilo" Publication Type Academic Journals
425 results on '"Jakob, Thilo"'

1. White paper on peanut allergy – part 1: Epidemiology, burden of disease, health economic aspects

Author

Lange, Lars, Klimek, Ludger, Beyer, Kirsten, Blümchen, Katharina, Novak, Natalija, Hamelmann, Eckard, Bauer, Andrea, Merk, Hans, Rabe, Uta, Jung, Kirsten, Schlenter, Wolfgang, Ring, Johannes, Chaker, Adam, Wehrmann, Wolfgang, Becker, Sven, Mülleneisen, Norbert, Nemat, Katja, Czech, Wolfgang, Wrede, Holger, Brehler, Randolf, Fuchs, Thomas, Jakob, Thilo, Ankermann, Tobias, Schmidt, Sebastian M., Gerstlauer, Michael, Zuberbier, Torsten, Spindler, Thomas, and Vogelberg, Christian

Published
2021
Full Text
View/download PDF

2. Messages for patients and relatives from the 2021 update of the guideline on acute therapy and management of anaphylaxis

Author

Ring, Johannes, Beyer, Kirsten, Biedermann, Tilo, Bircher, Andreas, Fischer, Matthias, Fuchs, Thomas, Heller, Axel, Hoffmann, Florian, Huttegger, Isidor, Jakob, Thilo, Klimek, Ludger, Kopp, Matthias V., Kugler, Claudia, Lange, Lars, Pfaar, Oliver, Rietschel, Ernst, Rueff, Franziska, Schnadt, Sabine, Seifert, Roland, Stöcker, Britta, Treudler, Regina, Vogelberg, Christian, Werfel, Thomas, Worm, Margitta, Sitter, Helmut, and Brockow, Knut

Published
2021
Full Text
View/download PDF

3. Practical handling of allergic reactions to COVID-19 vaccines: A position paper from German and Austrian Allergy Societies AeDA, DGAKI, GPA and ÖGAI

Author

Klimek, Ludger, Bergmann, Karl-Christian, Brehler, Randolf, Pfützner, Wolfgang, Zuberbier, Torsten, Hartmann, Karin, Jakob, Thilo, Novak, Natalija, Ring, Johannes, Merk, Hans, Hamelmann, Eckard, Ankermann, Tobias, Schmidt, Sebastian, Untersmayr, Eva, Hötzenecker, Wolfram, Jensen-Jarolim, Erika, Brockow, Knut, Mahler, Vera, and Worm, Margitta

Published
2021
Full Text
View/download PDF

4. Penicillin-Toleranzinduktion in der Schwangerschaft: Allergologische Stellungnahme zur Empfehlung der aktuellen Leitlinie Diagnostik und Therapie der Syphilis (AWMF-Register-Nr. 059-002)

Author

Wedi, Bettina, Aberer, Werner, Brockow, Knut, Dickel, Heinrich, Brehler, Randolf, Jakob, Thilo, Kreft, Burkhard, Mahler, Vera, Merk, Hans F., Mülleneisen, Norbert, Ott, Hagen, Pfützner, Wolfgang, Röseler, Stefani, Ruëff, Franziska, Sunderkötter, Cord, Trautmann, Axel, Treudler, Regina, Worm, Margitta, and Wurpts, Gerda

Published
2021
Full Text
View/download PDF

5. Severe allergic reactions after COVID-19 vaccination with the Pfizer/BioNTech vaccine in Great Britain and USA: Position statement of the German Allergy Societies: Medical Association of German Allergologists (AeDA), German Society for Allergology and Clinical Immunology (DGAKI) and Society for Pediatric Allergology and Environmental Medicine (GPA)

Author

Klimek, Ludger, Novak, Natalija, Hamelmann, Eckard, Werfel, Thomas, Wagenmann, Martin, Taube, Christian, Bauer, Andrea, Merk, Hans, Rabe, Uta, Jung, Kirsten, Schlenter, Wolfgang, Ring, Johannes, Chaker, Adam, Wehrmann, Wolfgang, Becker, Sven, Mülleneisen, Norbert, Nemat, Katja, Czech, Wolfgang, Wrede, Holger, Brehler, Randolf, Fuchs, Thomas, Jakob, Thilo, Ankermann, Tobias, Schmidt, Sebastian M., Gerstlauer, Michael, Vogelberg, Christian, Zuberbier, Thomas, Hartmann, Karin, and Worm, Margitta

Published
2021
Full Text
View/download PDF

7. Guideline (S2k) on acute therapy and management of anaphylaxis: 2021 update: S2k-Guideline of the German Society for Allergology and Clinical Immunology (DGAKI), the Medical Association of German Allergologists (AeDA), the Society of Pediatric Allergology and Environmental Medicine (GPA), the German Academy of Allergology and Environmental Medicine (DAAU), the German Professional Association of Pediatricians (BVKJ), the Society for Neonatology and Pediatric Intensive Care (GNPI), the German Society of Dermatology (DDG), the Austrian Society for Allergology and Immunology (ÖGAI), the Swiss Society for Allergy and Immunology (SGAI), the German Society of Anaesthesiology and Intensive Care Medicine (DGAI), the German Society of Pharmacology (DGP), the German Respiratory Society (DGP), the patient organization German Allergy and Asthma Association (DAAB), the German Working Group of Anaphylaxis Training and Education (AGATE)

Author

Ring, Johannes, Beyer, Kirsten, Biedermann, Tilo, Bircher, Andreas, Fischer, Matthias, Fuchs, Thomas, Heller, Axel, Hoffmann, Florian, Huttegger, Isidor, Jakob, Thilo, Klimek, Ludger, Kopp, Matthias V., Kugler, Claudia, Lange, Lars, Pfaar, Oliver, Rietschel, Ernst, Rueff, Franziska, Schnadt, Sabine, Seifert, Roland, Stöcker, Britta, Treudler, Regina, Vogelberg, Christian, Werfel, Thomas, Worm, Margitta, Sitter, Helmut, and Brockow, Knut

Published
2021
Full Text
View/download PDF

9. Anaphylaxie-Risiko bei der COVID-19-Impfung: Empfehlungen für das praktische Management

Author

Worm, Margitta, Ring, Johannes, Klimek, Ludger, Jakob, Thilo, Lange, Lars, Treudler, Regina, Beyer, Kirsten, Werfel, Thomas, Biedermann, Tilo, Bircher, Andreas, Fischer, Matthias, Fuchs, Thomas, Heller, Axel R., Hoffmann, Florian, Huttegger, Isidor, Kopp, Matthias Volkmar, Kugler, Claudia, Lommatzsch, Marek, Pfaar, Oliver, Rietschel, Ernst, Rueff, Franziska, Schnadt, Sabine, Seifert, Roland, Stöcker, Britta, Vogelberg, Christian, Sitter, Helmut, Gieler, Uwe, and Brockow, Knut

Published
2021
Full Text
View/download PDF

11. A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

Author

Ansotegui, Ignacio J., Melioli, Giovanni, Canonica, Giorgio Walter, Gómez, R. Maximiliano, Jensen-Jarolim, Erika, Ebisawa, Motohiro, Luengo, Olga, Caraballo, Luis, Passalacqua, Giovanni, Poulsen, Lars K., Savi, Eleonora, Zuberbier, Torsten, Villa, Elisa, Oppenheimer, John, Asero, Riccardo, Bernstein, Jonathan, Bousquet, Jean, Cardona, Victoria, Cox, Lindo, Demoly, Pascal, Ferreira, Fatima, Giavina Bianchi, Pedro, Gonzalez Diaz, Sandra, Jakob, Thilo, Kase Tanno, Luciana, Kleine-Tebbe, Jorg, Levin, Michael, Martin, Bryan, Matricardi, Paolo Maria, Monge Ortega, Olga Patricia, Morais Almeida, Mario, Nunes, Carlos, Ortega Martell, José Antonio, Pawankar, Ruby, Renz, Harald, Rosário Filho, Nelson, Rouadi, Philip, Ruiba, Alessia, Sampson, Hugh, Sánchez Borges, Mario, Scala, Enrico, Schmid-Grendelmeier, Peter, Senna, Gian-Enrico, Sisul, Juan Carlos, Tang, Mimi L.K., Valenta, Rudolf, van Hage, Marianne, Wong, Gary W.K., and Yáñez, Anahí

Published
2020
Full Text
View/download PDF

13. Worldwide perspectives on venom allergy

Author

Korošec, Peter, Jakob, Thilo, Harb, Harfi, Heddle, Robert, Karabus, Sarah, de Lima Zollner, Ricardo, Selb, Julij, Thong, Bernard Yu-Hor, Zaitoun, Fares, Golden, David B.K., and Levin, Michael

Published
2019
Full Text
View/download PDF

14. Prognostic impact of eosinophils in mastocytosis: analysis of 2350 patients collected in the ECNM Registry

Author

Kluin-Nelemans, Hanneke C., Reiter, Andreas, Illerhaus, Anja, van Anrooij, Bjorn, Hartmann, Karin, Span, Lambertus F. R., Gorska, Aleksandra, Niedoszytko, Marek, Lange, Magdalena, Scaffidi, Luigi, Zanotti, Roberta, Bonadonna, Patrizia, Perkins, Cecelia, Elena, Chiara, Malcovati, Luca, Shoumariyeh, Khalid, von Bubnoff, Nikolas, Parente, Roberta, Triggiani, Massimo, Schwaab, Juliana, Jawhar, Mohamad, Caroppo, Francesca, Fortina, Anna Belloni, Brockow, Knut, Zink, Alexander, Fuchs, David, Kilbertus, Alex, Yavuz, Akif Selim, Doubek, Michael, Mattsson, Mattias, Hagglund, Hans, Panse, Jens, Sabato, Vito, Aberer, Elisabeth, Niederwieser, Dietger, Breynaert, Christine, Várkonyi, Judit, Kennedy, Vanessa, Lortholary, Olivier, Jakob, Thilo, Hermine, Olivier, Rossignol, Julien, Arock, Michel, Gotlib, Jason, Valent, Peter, and Sperr, Wolfgang R.

Published
2020
Full Text
View/download PDF

15. ARIA guideline 2019: treatment of allergic rhinitis in the German health system

Author

Klimek, Ludger, Bachert, Claus, Pfaar, Oliver, Becker, Sven, Bieber, Thomas, Brehler, Randolf, Buhl, Roland, Casper, Ingrid, Chaker, Adam, Czech, Wolfgang, Fischer, Jörg, Fuchs, Thomas, Gerstlauer, Michael, Hörmann, Karl, Jakob, Thilo, Jung, Kirsten, Kopp, Matthias V., Mahler, Vera, Merk, Hans, Mülleneisen, Norbert, Nemat, Katja, Rabe, Uta, Ring, Johannes, Saloga, Joachim, Schlenter, Wolfgang, Schmidt-Weber, Carsten, Seyfarth, Holger, Sperl, Annette, Spindler, Thomas, Staubach, Petra, Strieth, Sebastian, Treudler, Regina, Vogelberg, Christian, Wallrafen, Andrea, Wehrmann, Wolfgang, Wrede, Holger, Zuberbier, Torsten, Bedbrook, Anna, Canonica, Giorgio W., Cardona, Victoria, Casale, Thomas B., Czarlewski, Wienczylawa, Fokkens, Wytske J., Hamelmann, Eckard, Hellings, Peter W., Jutel, Marek, Larenas-Linnemann, Désirée, Mullol, Joaquim, Papadopoulos, Nikolaos G., Toppila-Salmi, Sanna, Werfel, Thomas, and Bousquet, Jean

Published
2019
Full Text
View/download PDF

16. Hypomorphic homozygous mutations in phosphoglucomutase 3 (PGM3) impair immunity and increase serum IgE levels

Author

Sassi, Atfa, Lazaroski, Sandra, Wu, Gang, Haslam, Stuart M, Fliegauf, Manfred, Mellouli, Fethi, Patiroglu, Turkan, Unal, Ekrem, Ozdemir, Mehmet Akif, Jouhadi, Zineb, Khadir, Khadija, Ben-Khemis, Leila, Ben-Ali, Meriem, Ben-Mustapha, Imen, Borchani, Lamia, Pfeifer, Dietmar, Jakob, Thilo, Khemiri, Monia, Asplund, A Charlotta, Gustafsson, Manuela O, Lundin, Karin E, Falk-Sörqvist, Elin, Moens, Lotte N, Gungor, Hatice Eke, Engelhardt, Karin R, Dziadzio, Magdalena, Stauss, Hans, Fleckenstein, Bernhard, Meier, Rebecca, Prayitno, Khairunnadiya, Maul-Pavicic, Andrea, Schaffer, Sandra, Rakhmanov, Mirzokhid, Henneke, Philipp, Kraus, Helene, Eibel, Hermann, Kölsch, Uwe, Nadifi, Sellama, Nilsson, Mats, Bejaoui, Mohamed, Schäffer, Alejandro A, Smith, CI Edvard, Dell, Anne, Barbouche, Mohamed-Ridha, and Grimbacher, Bodo

Subjects
Biomedical and Clinical Sciences, Immunology, Clinical Research, Genetics, Rare Diseases, Emerging Infectious Diseases, Aetiology, 2.1 Biological and endogenous factors, Adult, Amino Acid Substitution, Cell Proliferation, Child, Chromosomes, Human, Pair 6, Female, Genetic Diseases, Inborn, Genetic Linkage, Glycosylation, Homozygote, Humans, Immunity, Immunoglobulin E, Infant, Job Syndrome, Male, Mutation, Missense, Phosphoglucomutase, T-Lymphocytes, Tunisia, Hyper-IgE syndrome, glycosylation, Staphylococcus aureus, signal transducer and activator of transcription 3, dedicator of cytokinesis 8, phosphoglucomutase 3, Allergy
Abstract

BackgroundRecurrent bacterial and fungal infections, eczema, and increased serum IgE levels characterize patients with the hyper-IgE syndrome (HIES). Known genetic causes for HIES are mutations in signal transducer and activator of transcription 3 (STAT3) and dedicator of cytokinesis 8 (DOCK8), which are involved in signal transduction pathways. However, glycosylation defects have not been described in patients with HIES. One crucial enzyme in the glycosylation pathway is phosphoglucomutase 3 (PGM3), which catalyzes a key step in the synthesis of uridine diphosphate N-acetylglucosamine, which is required for the biosynthesis of N-glycans.ObjectiveWe sought to elucidate the genetic cause in patients with HIES who do not carry mutations in STAT3 or DOCK8.MethodsAfter establishing a linkage interval by means of SNPchip genotyping and homozygosity mapping in 2 families with HIES from Tunisia, mutational analysis was performed with selector-based, high-throughput sequencing. Protein expression was analyzed by means of Western blotting, and glycosylation was profiled by using mass spectrometry.ResultsMutational analysis of candidate genes in an 11.9-Mb linkage region on chromosome 6 shared by 2 multiplex families identified 2 homozygous mutations in PGM3 that segregated with disease status and followed recessive inheritance. The mutations predict amino acid changes in PGM3 (p.Glu340del and p.Leu83Ser). A third homozygous mutation (p.Asp502Tyr) and the p.Leu83Ser variant were identified in 2 other affected families, respectively. These hypomorphic mutations have an effect on the biosynthetic reactions involving uridine diphosphate N-acetylglucosamine. Glycomic analysis revealed an aberrant glycosylation pattern in leukocytes demonstrated by a reduced level of tri-antennary and tetra-antennary N-glycans. T-cell proliferation and differentiation were impaired in patients. Most patients had developmental delay, and many had psychomotor retardation.ConclusionImpairment of PGM3 function leads to a novel primary (inborn) error of development and immunity because biallelic hypomorphic mutations are associated with impaired glycosylation and a hyper-IgE-like phenotype.

Published
2014

17. Efficacy and safety of on‐demand versus daily rupatadine in chronic spontaneous urticaria: A randomized trial.

Author

Weller, Karsten, Gimenez‐Arnau, Ana Maria, Baron, Jens, Brehler, Randolf, Ferrer, Marta, Groffik, Adriane, Grundmann, Sonja, Jakob, Thilo, Labrador‐Horrillo, Moisés, Müller, Sabine, Staubach, Petra, Wurpts, Gerda, Metz, Martin, and Maurer, Marcus

Subjects
URTICARIA, PREVENTIVE medicine
Abstract

Background: Non‐sedating H1‐antihistamines (nsAH) are the most commonly used treatment for chronic spontaneous urticaria (CSU). Many patients use them as on‐demand (OD) therapy rather than a maintenance treatment. Here, we compared OD versus daily maintenance treatment with the nsAH rupatadine, assessed the efficacy of rupatadine updosing, and investigated potential long‐term disease‐modifying effects. Methods: This multicenter, randomized study consisted of 2 weeks of screening, 8 weeks of double‐blind treatment, and 6 weeks of treatment‐free follow‐up (OD allowed). Adult patients were randomized to 10 mg rupatadine OD or 10 mg rupatadine daily. At Week 4, if patients did not have a complete response, they switched from 10 to 20 mg rupatadine daily or underwent sham updosing (patients on 10 mg rupatadine OD). The primary aim was to compare CSU disease activity at the end of follow‐up between daily versus OD. Additionally, we assessed the efficacy of rupatadine updosing. Major outcomes were disease activity, CSU‐related quality of life (QoL), and disease control. Results: At Week 4, disease activity and QoL significantly improved in daily versus OD‐treated patients. Updosing of rupatadine did not improve the mean disease activity, but the number of complete responders increased during updosing from 5% to 22%. At the end of follow‐up, the disease activity of patients treated OD versus daily was not significantly different. Conclusions: Daily rupatadine treatment significantly improved CSU disease activity and QoL during treatment versus OD treatment but not after discontinuation of rupatadine, indicating the benefits of a daily maintenance nsAH schedule. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

18. Correction to “ARIA guideline 2019: treatment of allergic rhinitis in the German health system”

Author

Klimek, Ludger, Bachert, Claus, Pfaar, Oliver, Becker, Sven, Bieber, Thomas, Brehler, Randolf, Buhl, Roland, Casper, Ingrid, Chaker, Adam, Czech, Wolfgang, Fischer, Jörg, Fuchs, Thomas, Gerstlauer, Michael, Hörmann, Karl, Jakob, Thilo, Jung, Kirsten, Kopp, Matthias V., Mahler, Vera, Merk, Hans, Mülleneisen, Norbert, Nemat, Katja, Rabe, Uta, Ring, Johannes, Saloga, Joachim, Schlenter, Wolfgang, Schmidt-Weber, Carsten, Seyfarth, Holger, Sperl, Annette, Spindler, Thomas, Staubach, Petra, Strieth, Sebastian, Treudler, Regina, Vogelberg, Christian, Wallrafen, Andrea, Wehrmann, Wolfgang, Wrede, Holger, Zuberbier, Torsten, Bedbrook, Anna, Canonica, Giorgio W., Cardona, Victoria, Casale, Thomas B., Czarlewski, Wienczylawa, Fokkens, Wytske J., Hamelmann, Eckard, Hellings, Peter W., Jutel, Marek, Larenas-Linnemann, Désirée, Mullol, Joaquim, Papadopoulos, Nikolaos G., Toppila-Salmi, Sanna, Werfel, Thomas, and Bousquet, Jean

Published
2020
Full Text
View/download PDF

21. Guideline on diagnostic procedures for suspected hypersensitivity to beta-lactam antibiotics: Guideline of the German Society for Allergology and Clinical Immunology (DGAKI) in collaboration with the German Society of Allergology (AeDA), German Society for Pediatric Allergology and Environmental Medicine (GPA), the German Contact Dermatitis Research Group (DKG), the Austrian Society for Allergology and Immunology (ÖGAI), and the Paul-Ehrlich Society for Chemotherapy (PEG)

Author

Wurpts, Gerda, Aberer, Werner, Dickel, Heinrich, Brehler, Randolf, Jakob, Thilo, Kreft, Burkhard, Mahler, Vera, Merk, Hans F., Mülleneisen, Norbert, Ott, Hagen, Pfützner, Wolfgang, Röseler, Stefani, Ruëff, Franziska, Sitter, Helmut, Sunderkötter, Cord, Trautmann, Axel, Treudler, Regina, Wedi, Bettina, Worm, Margitta, and Brockow, Knut

Published
2019
Full Text
View/download PDF

30. In chronic spontaneous urticaria soluble FcεRI is elevated and linked to atopy and chronic inducible urticaria.

Author

Moñino‐Romero, Sherezade, Kolkhir, Pavel, Szépfalusi, Zsolt, Schoepke, Nicole, Metz, Martin, Asero, Riccardo, Ferrer, Marta, Gimenez‐Arnau, Ana, Grattan, Clive E. H., Jakob, Thilo, Konstantinou, George N., Raap, Ulrike, Staubach, Petra, Zhang, Ke, Bindslev‐Jensen, Carsten, Daschner, Alvaro, Kinaciyan, Tamar, Makris, Michael, Marrouche, Nadine, and Schmid‐Grendelmeier, Peter

Subjects
URTICARIA, ATOPY, AUTOANTIBODIES, IMMUNOGLOBULIN E, MAST cells, CHILD patients, BLOOD cells
Abstract

Elevated total IgE has been proposed as a biomarker, but study results are inconsistent[[3]] and total IgE levels in autoallergic CSU may be elevated due to comorbid sensitization rather than the presence of pathogenetically relevant IgE autoantibodies. SFc RI levels need to be assessed and compared in CSU patients with and without IgE autoantibodies, and human skin MCs should be investigated for their release of sFc RI following activation by IgE versus IgG autoantibodies. Based on our findings, we hypothesize that sFc RI may be a suitable marker for autoallergic CSU since it is solely released upon IgE-mediated crosslinking, that is, in patients with relevant IgE sensitization. [Extracted from the article]

Published
2023
Full Text
View/download PDF

33. Correction to: Guideline for the diagnosis of drug hypersensitivity reactions

Author

Brockow, Knut, Przybilla, Bernhard, Aberer, Werner, Bircher, Andreas J., Brehler, Randolf, Dickel, Heinrich, Fuchs, Thomas, Jakob, Thilo, Lange, Lars, Pfützner, Wolfgang, Mockenhaupt, Maja, Ott, Hagen, Pfaar, Oliver, Ring, Johannes, Sachs, Bernhardt, Sitter, Helmut, Trautmann, Axel, Treudler, Regina, Wedi, Bettina, Worm, Margitta, Wurpts, Gerda, Zuberbier, Torsten, and Merk, Hans F.

Published
2017
Full Text
View/download PDF

37. Supportive care of patients with anaphylaxis — options and shortcomings: an assessment on behalf of the working group on anaphylaxis training and education (AGATE), Germany

Author

Brockow, Knut, Beyer, Kirsten, Biedermann, Tilo, Fischer, Jörg, Gieler, Uwe, Giessler-Fichtner, Oliver, Gebert, Norbert, Jakob, Thilo, Klimek, Ludger, Kugler, Claudia, Reese, Imke, Rietschel, Ernst, Ruëff, Franziska, Schnadt, Sabine, Siemens, Cäcilia, Szczepanski, Rüdiger, Worm, Margitta, Ring, Johannes, Lange, Lars, and the working group on anaphylaxis training and education (AGATE), Germany

Published
2016
Full Text
View/download PDF

43. Stimulation of naïve B cells with a fusion protein consisting of FlaA and Bet v 1 induces regulatory B cells ex vivo.

Author

Goretzki, Alexandra, Lin, Yen‐Ju, Meier, Clara, Dorn, Britta, Wolfheimer, Sonja, Jamin, Annette, Schott, Maike, Wangorsch, Andrea, Vieths, Stefan, Jakob, Thilo, Scheurer, Stephan, and Schülke, Stefan

Subjects
REGULATORY B cells, B cells, CHIMERIC proteins, CELL fusion, IMMUNOREGULATION, ANTIBODY formation
Abstract

Background: The experimental fusion protein rFlaA:Betv1 was shown to efficiently suppress allergen‐specific sensitization in mice. However, the detailed mechanism of rFlaA:Betv1‐mediated immune modulation is not fully understood. In this study, we investigated the effect of rFlaA:Betv1 on naïve murine B cells. Methods: Immune modulating capacity of rFlaA:Betv1 was screened in IL‐10 reporter mice. B cells were isolated from spleens of naïve C57Bl/6, TLR5−/−, or MyD88−/− mice, stimulated with rFlaA:Betv1 and controls, and monitored for the expression of the regulatory B cell markers CD1d, CD24, CD38, and surface IgM by flow cytometry. Secreted cytokines, antibodies, and reactivity of the induced antibodies were investigated by ELISA and intracellular flow cytometry. Suppressive capacity of rFlaA:Betv1‐stimulated B cells was tested in mDC:CD4+ T cell:B cell triple cultures. Results: Upon in vivo application of rFlaA:Betv1 into IL‐10‐GFP reporter mice, CD19+ B cells were shown to produce anti‐inflammatory IL‐10, suggesting B cells to contribute to the immune‐modulatory properties of rFlaA:Betv1. rFlaA:Betv1‐induced IL‐10 secretion was confirmed in human B cells isolated from buffy coats. In vitro stimulation of naïve murine B cells with rFlaA:Betv1 resulted in an mTOR‐ and MyD88‐dependent production of IL‐10 and rFlaA:Betv1 induced Bet v 1‐reactive IgG production, which was not observed for IgA. rFlaA:Betv1‐stimulated B cells formed a CD19+CD24+CD1d+IgM+CD38+ Breg subpopulation capable of suppressing Bet v 1‐induced TH2 cytokine secretion in vitro. Conclusion: rFlaA:Betv1 can act as a thymus‐independent B cell antigen, stimulating the mTOR‐ and MyD88‐dependent differentiation of B cells displaying a regulatory phenotype, IL‐10 secretion, antigen‐binding antibody production, and a suppressive capacity in vitro. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

44. Structural and functional analyses of antibodies specific for modified core N‐glycans suggest a role in TH2 responses.

Author

Plum, Melanie, Tjerrild, Luna, Raiber, Tim, Bantleon, Frank, Bantleon, Sara, Miehe, Michaela, Jabs, Frederic, Seismann, Henning, Möbs, Christian, Pfützner, Wolfgang, Jakob, Thilo, Andersen, Gregers R., and Spillner, Edzard

Subjects
IMMUNOGLOBULINS, FUNCTIONAL analysis, ANTIGEN presentation, SURFACE plasmon resonance, GLYCOPROTEINS
Abstract

Background: Immune responses to N‐glycan structures from allergens and parasites are often associated with pronounced, high affinity IgE reactivities. Cross‐reactive carbohydrate determinants (CCDs) are constituted by modified N‐glycan core structures and represent the most frequently recognized epitopes in allergic immune responses. Although recently accepted as potentially allergenic epitopes, the biological and clinical relevance as well as structural and functional characteristics of CCD‐specific antibodies remain elusive. Methods: In order to gain structural insights into the recognition of CCDs, two specific antibody fragments were isolated from a leporid immune repertoire library and converted into human/leporid IgE and IgG formats. The antibody formats were assessed by ELISA and surface plasmon resonance, structural and functional analyses were performed by X‐ray crystallography, mediator release, and ELIFAB assays. Results: The recombinant IgE exhibited highly specific interactions with different types of CCDs on numerous CCD‐carrying glycoproteins. Crystal structures of two CCD‐specific antibodies, one of which in complex with a CCD‐derived disaccharide emphasize that mechanisms of core glycan epitope recognition are as specific as those governing protein epitope recognition. The rIgE triggered immediate cellular responses via FcεRI cross‐linking and mediated facilitated antigen presentation by binding of IgE/antigen complexes to CD23, a process that also could be blocked by IgG of allergic patients. Conclusions: Our study provides evidence for the relevance of N‐glycan recognition in TH2 responses and corroborates that IgE and IgG antibodies to ubiquitous carbohydrate epitopes can be equivalent to those directed against proteinaceous epitopes with implications for diagnostic and immunotherapeutic concepts. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

45. Guideline for the diagnosis of drug hypersensitivity reactions: S2K-Guideline of the German Society for Allergology and Clinical Immunology (DGAKI) and the German Dermatological Society (DDG) in collaboration with the Association of German Allergologists (AeDA), the German Society for Pediatric Allergology and Environmental Medicine (GPA), the German Contact Dermatitis Research Group (DKG), the Swiss Society for Allergy and Immunology (SGAI), the Austrian Society for Allergology and Immunology (ÖGAI), the German Academy of Allergology and Environmental Medicine (DAAU), the German Center for Documentation of Severe Skin Reactions and the German Federal Institute for Drugs and Medical Products (BfArM)

Author

Brockow, Knut, Przybilla, Bernhard, Aberer, Werner, Bircher, Andreas J., Brehler, Randolf, Dickel, Heinrich, Fuchs, Thomas, Jakob, Thilo, Lange, Lars, Pfützner, Wolfgang, Mockenhaupt, Maja, Ott, Hagen, Pfaar, Oliver, Ring, Johannes, Sachs, Bernhardt, Sitter, Helmut, Trautmann, Axel, Treudler, Regina, Wedi, Bettina, Worm, Margitta, Wurpts, Gerda, Zuberbier, Torsten, and Merk, Hans F.

Published
2015
Full Text
View/download PDF

50. Guideline for acute therapy and management of anaphylaxis: S2 Guideline of the German Society for Allergology and Clinical Immunology (DGAKI), the Association of German Allergologists (AeDA), the Society of Pediatric Allergy and Environmental Medicine (GPA), the German Academy of Allergology and Environmental Medicine (DAAU), the German Professional Association of Pediatricians (BVKJ), the Austrian Society for Allergology and Immunology (ÖGAI), the Swiss Society for Allergy and Immunology (SGAI), the German Society of Anaesthesiology and Intensive Care Medicine (DGAI), the German Society of Pharmacology (DGP), the German Society for Psychosomatic Medicine (DGPM), the German Working Group of Anaphylaxis Training and Education (AGATE) and the patient organization German Allergy and Asthma Association (DAAB)

Author

Ring, Johannes, Beyer, Kirsten, Biedermann, Tilo, Bircher, Andreas, Duda, Dorothea, Fischer, Jörg, Friedrichs, Frank, Fuchs, Thomas, Gieler, Uwe, Jakob, Thilo, Klimek, Ludger, Lange, Lars, Merk, Hans F., Niggemann, Bodo, Pfaar, Oliver, Przybilla, Bernhard, Ruëff, Franziska, Rietschel, Ernst, Schnadt, Sabine, Seifert, Roland, Sitter, Helmut, Varga, Eva-Maria, Worm, Margitta, and Brockow, Knut

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results