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As an alternative to fossil-based polymers, polylactide acid (PLA) has stimulated a significant research effort in the past few years due to the demand for environmentally friendly products. Even though PLA is considered a sustainable or bio-based material, the long-term degradation in ambient conditions increases the volume of waste at end-of-life. To overcome this issue, PLA waste can be recycled to produce new manufactures; however, this approach does not always ensure the same mechanical properties as the original PLA. This study shows for the first time that adding biochar, a carbon material derived from biomass pyrolysis, enhances the processability and stability of composite recycled PLA. Composites are provided in 1, 2.5, and 5 wt% of the biochar filler, resulting in good processability, a higher modulus of up to 20%, and a higher stability to degradation in the presence of UV aging treatment with respect to the raw material. Additionally, DSC analysis shows a significant nucleation effect induced by the biochar that achieves 30% crystallinity from an essentially amorphous PLA.

Sonochemical synthesis can be a facile, fast, efficient, versatile and economical way to prepare a large variety of conventional or novel nanostructured materials (metallic, magnetic, semiconducting, polymeric, etc.). In this work, zinc oxide nanocrystals were synthesized by irradiating and heating at 90 °C in a commercial ultrasonic bath a water solution of zinc nitrate hexahydrate and ammonia solution or hexamethylenetetramine as base catalysts. The evolution of the powder morphology and its crystalline structure were investigated at different times of ultrasonic irradiation (0-9 hours) and compared with those of samples obtained by only heating the solutions in a muffle furnace in order to enlighten the growth mechanism. It resulted that: i) the crystal morphology depends on the selected base, ii) for samples obtained by using ultrasounds, the homogeneity of the powders depends on the irradiation time, iii) by comparing all samples obtained at 7 hours of heating, the aspect ratio of the crystals is higher for those that also underwent to ultrasounds., (© 2024 Wiley-VCH GmbH.)

Bioinspired strategies for scaffold design and optimization were improved by the introduction of Additive Manufacturing (AM), thus allowing for replicating and reproducing complex shapes and structures in a reliable manner, adopting different kinds of polymeric and nanocomposite materials properly combined according to the features of the natural host tissues. Benefiting from recent findings in AM, a Matrix-Assisted Pulsed Laser Evaporation (MAPLE) technique was employed for obtaining graphene-like material (GL) uniform coatings on 3D scaffolds for tissue repair strategies, towards the development of a new concept 3D scaffold with controlled morphological/architectural and surface features and mechanical and biological properties. The effect of the material-design combination through an integrated technological approach ( i.e. , MAPLE deposition of GL on 3D AM PCL scaffolds) was assessed through scanning electron microscopy, atomic force microscopy, contact angle measurements, mechanical measurements and biological analyses (cell viability assay and alkaline phosphatase activity) in conjunction with confocal laser scanning microscopy. The differentiation of hMSCs towards the osteoblast phenotype was also investigated analysing the gene expression profile. The obtained findings provided a further insight into the development of improved strategies for the functionalization or combination of GL with other materials and 3D structures in a hybrid fashion for ensuring a tighter adhesion onto the substrates, improving cell fate over time, without negatively altering the mechanical properties and behaviour of the neat constructs. In particular, the results provided interesting information, making 3D AM GL-coated scaffolds potential candidates for bone tissue engineering., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

The combination of soil amendments with plants can be a viable option for restoring the functionality of PTEs-contaminated soils. Soil recovery could be further optimized through the mixed cropping of plant species (e.g. legumes and grasses) with different physiological characteristics. The aim of this study was to assess the phytoremediation ability of Vicia villosa Roth. And Lolium rigidum Gaud. Grown alone or in mixture in a soil contaminated with PTEs (C), i.e. Cd (23 mg kg -1 ), Pb (4473 mg kg -1 ) and Zn (3147 mg kg -1 ), and amended with 3% biochar (C + B). Biochar improved soil fertility and changed PTEs distribution, reducing soluble fractions and increasing the more stable ones. The addition of biochar increased the plant biomass of hairy vetch and annual ryegrass, both in monoculture and when in mixture. For example, shoot and root biomass of the C + B intercropped hairy vetch and annual ryegrass increased 9- and 7-fold, and ∼3-fold respectively, compared to the respective C plants. The biochar addition decreased PTE-uptake by both plants, while mixed cropping increased the uptake of PTEs by shoots of hairy vetch grown in C and C + B. The bioaccumulation, translocation factors, and mineralomass showed that hairy vetch and annual ryegrass behaved as phytostabilising plants. PTE mineralomasses proved that mixed cropping in C + B increased the overall capacity of PTE accumulation by plant tissues, particularly the root system. Therefore, the combination of biochar and legumes/grasses mixed cropping could be an effective solution for the recovery of PTEs-contaminated soils and the mitigation of their environmental hazard., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Soil Surface Roughness (SSR) is a physical feature of soil microtopography, which is strongly influenced by tillage practices and plays a key role in hydrological and soil erosion processes. Therefore, surface roughness indices are required when using models to estimate soil erosion rates, where tabular values or direct measurements can be used. Field measurements often imply out-of-date and time-consuming methods, such as the pin meter and the roller chain, providing inaccurate indices. A novel technique for SSR measurement has been adopted, employing an RGB-Depth camera to produce a small-scale Digital Elevation Model of the soil surface, in order to extrapolate roughness indices. Canopy cover coverage (CC) of the cover crop was also detected from the camera's images. The values obtained for SSR and CC indices were implemented in the MMF (Morgan-Morgan-Finney) model, to validate the reliability of the proposed methodology by comparing the models' results for sediment yields with long-term soil erosion measurements in sloping vineyards in NW Italy. The performance of the model in predicting soil losses was satisfactory to good for a vineyard plot with inter-rows managed with recurrent tillage, and it was improved using spatialized soil roughness input data with respect to a uniform value. Performance for plot with permanent ground cover was not so good, however it was also improved using spatialized data. The measured values were also useful to obtain C-factor for RUSLE application, to be used instead of tabular values., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

This study investigated the antibiofilm activity of water-soluble extracts obtained under different pH conditions from Cannabis sativa seeds and from previously defatted seeds. The chemical composition of the extracts, determined through GC-MS and NMR, revealed complex mixtures of fatty acids, monosaccharides, amino acids and glycerol in ratios depending on extraction pH. In particular, the extract obtained at pH 7 from defatted seeds (Ex7d) contained a larger variety of sugars compared to the others. Saturated and unsaturated fatty acids were found in all of the analysed extracts, but linoleic acid (C18:2) was detected only in the extracts obtained at pH 7 and pH 10. The extracts did not show cytotoxicity to HaCaT cells and significantly inhibited the formation of Staphylococcus epidermidis biofilms. The exception was the extract obtained at pH 10, which appeared to be less active. Ex7d showed the highest antibiofilm activity, i.e., around 90%. Ex7d was further fractionated by HPLC, and the antibiofilm activity of all fractions was evaluated. The 2D-NMR analysis highlighted that the most active fraction was largely composed of glycerolipids. This evidence suggested that these molecules are probably responsible for the observed antibiofilm effect but does not exclude a possible synergistic contribution by the other components.

β-Glucosidase (BG) catalyzes the hydrolysis of cellobiose to glucose, a substrate for fermentation to produce the carbon-neutral fuel bioethanol. Enzyme thermal stability and reusability can be improved through immobilization onto insoluble supports. Moreover, nanoscaled matrixes allow for preserving high reaction rates. In this work, BG was physically immobilized onto wrinkled SiO 2 nanoparticles (WSNs). The adsorption procedure was tuned by varying the BG:WSNs weight ratio to achieve the maximum controllability and maximize the yield of immobilization, while different times of immobilization were monitored. Results show that a BG:WSNs ratio equal to 1:6 wt/wt provides for the highest colloidal stability, whereas an immobilization time of 24 h results in the highest enzyme loading (135 mg/g of support) corresponding to 80% yield of immobilization. An enzyme corona is formed in 2 h, which gradually disappears as the protein diffuses within the pores. The adsorption into the silica structure causes little change in the protein secondary structure. Furthermore, supported enzyme exhibits a remarkable gain in thermal stability, retaining complete folding up to 90 °C. Catalytic tests assessed that immobilized BG achieves 100% cellobiose conversion. The improved adsorption protocol provides simultaneously high glucose production, enhanced yield of immobilization, and good reusability, resulting in considerable reduction of enzyme waste in the immobilization stage.

Tractor rollover is the main cause of both fatal and non-fatal injuries in agriculture. The foldable rollover protective structure (FROPS) can help to prevent these injuries. However, in many cases, the FROPS is left in a folded-down position. Human factor and rear-mounted FROPS technical characteristics influence operators' behavior in roll-bar handling. To improve the FROPS's comfortable use, the prototype of an enhanced handling system was developed, and its usability was tested and compared with a conventional FROPS. Usability was assessed with 93 novice tractor users through an ad hoc questionnaire (investigating perceived effort, time demand, the posture adopted and satisfaction) and observations (investigating effectiveness and efficiency) during lowering and raising tasks. For both tasks, the participants perceived significantly less effort, less physical discomfort, a higher level of satisfaction and less time demand while operating the enhanced FROPS. Observations showed that the critical issues that emerged for the conventional FROPS were eliminated by adopting the developed and implemented handling system. The developed handling system showed itself to be usable and effective in making the FROPS easier and safer to be operated.

Understanding linkages between heterogeneous soil structures and non-uniform flow is fundamental for interpreting infiltration processes and improving hydrological simulations. Here, we utilized ground-penetrating radar (GPR) as a non-invasive technique to investigate those linkages and to complement current traditional methods that are labor-intensive, invasive, and non-repeatable. We combined time-lapse GPR surveys with different types of infiltration experiments to create three-dimensional (3D) diagrams of the wetting dynamics. We carried out the GPR surveys and validated them with in situ observations, independent measurements and field excavations at two experimental sites. Those sites were selected to represent different mechanisms that generate non-uniform flow: (1) preferential water infiltration initiated by tree trunk and root systems; and (2) lateral subsurface flow due to soil layering. Results revealed links between different types of soil heterogeneity and non-uniform flow. The first experimental site provided evidence of root-induced preferential flow paths along coarse roots, emphasizing the important role of coarse roots in facilitating preferential water movement through the subsurface. The second experimental site showed that water infiltrated through the restrictive layer mainly following the plant root system. The presented approach offers a non-invasive, repeatable and accurate way to detect non-uniform flow., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Introduction: Occupational safety and health (OSH) have a remarkable impact on the sustainability of firms and organizations. However, the sustainability perspective has often focused primarily on environmental and economic concerns, leaving key social and workplace aspects, such as OSH, underemphasized. The link between all these aspects is particularly relevant in agriculture., Method: In the present study we analyzed the paths by which the worker's age, participation in safety training, and previous history of accidents and near misses can influence the adoption of safety behaviors in agriculture through the mediation of attitudes towards safety and perceived behavioral control. Three hundred and ten Italian agricultural operators were administered an ad-hoc questionnaire., Results: Having attended safety training courses, having been exposed to previous accidents and near misses, and age showed a positive association with the adoption of on-farm safety behaviors, through the mediation of perceived behavioral control. In more detail, participation in training courses and age showed a positive association with perceived behavioral control, while the previous history of accidents and near misses showed a negative association with behavioral control. In turn, perceived behavioral control was positively associated with the adoption of safety behaviors., Conclusions: The present study focused on the chain of events leading to the adoption of on-farm safety behaviors, which can prevent an accidents, pointing out the more critical variables and the level at which they intervene, identifying possible areas of targeted interventions. Practical applications: Implications for the development of targeted interventions may deal with: (a) training activities increasing farm operators' sense of control over their safety, (b) older farmers involvement in mentoring initiatives, and (c) systematic recording and use of previous history of accidents and near misses as inputs for training activities to discuss their implications for on-farm safety., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 National Safety Council and Elsevier Ltd. All rights reserved.)

To gain a better understanding of the global application of soil erosion prediction models, we comprehensively reviewed relevant peer-reviewed research literature on soil-erosion modelling published between 1994 and 2017. We aimed to identify (i) the processes and models most frequently addressed in the literature, (ii) the regions within which models are primarily applied, (iii) the regions which remain unaddressed and why, and (iv) how frequently studies are conducted to validate/evaluate model outcomes relative to measured data. To perform this task, we combined the collective knowledge of 67 soil-erosion scientists from 25 countries. The resulting database, named 'Global Applications of Soil Erosion Modelling Tracker (GASEMT)', includes 3030 individual modelling records from 126 countries, encompassing all continents (except Antarctica). Out of the 8471 articles identified as potentially relevant, we reviewed 1697 appropriate articles and systematically evaluated and transferred 42 relevant attributes into the database. This GASEMT database provides comprehensive insights into the state-of-the-art of soil- erosion models and model applications worldwide. This database intends to support the upcoming country-based United Nations global soil-erosion assessment in addition to helping to inform soil erosion research priorities by building a foundation for future targeted, in-depth analyses. GASEMT is an open-source database available to the entire user-community to develop research, rectify errors, and make future expansions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)

Soil erosion can present a major threat to agriculture due to loss of soil, nutrients, and organic carbon. Therefore, soil erosion modelling is one of the steps used to plan suitable soil protection measures and detect erosion hotspots. A bibliometric analysis of this topic can reveal research patterns and soil erosion modelling characteristics that can help identify steps needed to enhance the research conducted in this field. Therefore, a detailed bibliometric analysis, including investigation of collaboration networks and citation patterns, should be conducted. The updated version of the Global Applications of Soil Erosion Modelling Tracker (GASEMT) database contains information about citation characteristics and publication type. Here, we investigated the impact of the number of authors, the publication type and the selected journal on the number of citations. Generalized boosted regression tree (BRT) modelling was used to evaluate the most relevant variables related to soil erosion modelling. Additionally, bibliometric networks were analysed and visualized. This study revealed that the selection of the soil erosion model has the largest impact on the number of publication citations, followed by the modelling scale and the publication's CiteScore. Some of the other GASEMT database attributes such as model calibration and validation have negligible influence on the number of citations according to the BRT model. Although it is true that studies that conduct calibration, on average, received around 30% more citations, than studies where calibration was not performed. Moreover, the bibliographic coupling and citation networks show a clear continental pattern, although the co-authorship network does not show the same characteristics. Therefore, soil erosion modellers should conduct even more comprehensive review of past studies and focus not just on the research conducted in the same country or continent. Moreover, when evaluating soil erosion models, an additional focus should be given to field measurements, model calibration, performance assessment and uncertainty of modelling results. The results of this study indicate that these GASEMT database attributes had smaller impact on the number of citations, according to the BRT model, than anticipated, which could suggest that these attributes should be given additional attention by the soil erosion modelling community. This study provides a kind of bibliographic benchmark for soil erosion modelling research papers as modellers can estimate the influence of their paper., (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)

Digital games have been successfully applied in different working sectors as an occupational safety training method, but with a very limited application in agriculture. In agriculture and other productive sectors, unintentional injuries tend to occur with similar dynamics. A literature review was carried out to understand how occupational risks are addressed during game-based safety training in different productive sectors and how this can be transferred to agriculture. Literature about "serious game" and "gamification" as safety training methods was searched in WEB OF SCIENCE, SCOPUS, PUBMED and PsycINFO databases. In the forty-two publications retained, the computer was identified as the most adopted game support, whereas "points", "levels", "challenges" and "discovery" were the preferred game mechanics. Moreover, an association can be detected between the game mechanics and the elements developed in the game. Finally, during the game assessment, much positive feedback was collected and the games proved to be able to increase the operators' skills and safety knowledge. In light of the results, insights are provided to develop an effective, satisfying and engaging safety game training for workers employed in agriculture. Games can be best used to learn and they are certain to improve over the next few years.