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Your search keyword '"Ide, Toshinori"' showing total 161 results
Start Over Author "Ide, Toshinori" Publication Type Academic Journals
161 results on '"Ide, Toshinori"'

25. MOV10 as a novel telomerase-associated protein

Author

Nakano, Mariko, Kakiuchi, Yasutaka, Shimada, Yukiko, Ohyama, Mika, Ogiwara, Yasuko, Sasaki-Higashiyama, Narie, Yano, Naoko, Ikeda, Fumi, Yamada, Ei, Iwamatsu, Akihiro, Kobayashi, Kazuo, Nishiyama, Kiyoto, Ichikawa, Sohsuke, Kaji, Kazuhiko, Ide, Toshinori, Murofushi, Hiromu, and Murakami-Murofushi, Kimiko

Published
2009
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28. Mitogenic signalling and the p16INK4a–Rb pathway cooperate to enforce irreversible cellular senescence.

Author

Takahashi, Akiko, Ohtani, Naoko, Yamakoshi, Kimi, Iida, Shin-ichi, Tahara, Hidetoshi, Nakayama, Keiko, Nakayama, Keiichi I., Ide, Toshinori, Saya, Hideyuki, and Hara, Eiji

Subjects
PROTEIN kinases, CYTOPLASM, TUMORS, RETINOBLASTOMA, PHOTOSYNTHETIC oxygen evolution, PROTEIN kinase C, CYTOKINESIS
Abstract

The p16INK4a cyclin-dependent kinase inhibitor has a key role in establishing stable G1 cell-cycle arrest through activating the retinoblastoma (Rb) tumour suppressor protein pRb in cellular senescence. Here, we show that the p16INK4a /Rb-pathway also cooperates with mitogenic signals to induce elevated intracellular levels of reactive oxygen species (ROS), thereby activating protein kinase Cδ (PKCδ) in human senescent cells. Importantly, once activated by ROS, PKCδ promotes further generation of ROS, thus establishing a positive feedback loop to sustain ROS–PKCδ signalling. Sustained activation of ROS–PKCδ signalling irreversibly blocks cytokinesis, at least partly through reducing the level of WARTS (also known as LATS1), a mitotic exit network (MEN) kinase required for cytokinesis, in human senescent cells. This irreversible cytokinetic block is likely to act as a second barrier to cellular immortalization ensuring stable cell-cycle arrest in human senescent cells. These results uncover an unexpected role for the p16INK4a–Rb pathway and provide a new insight into how senescent cell-cycle arrest is enforced in human cells. [ABSTRACT FROM AUTHOR]

Published
2006
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29. In Vitro Establishment of Tumorigenic Human B-Lymphoblastoid Cell Lines Transformed by Epstein-Barr Virus.

Author

Takahashi, Tomoko, Kawabe, Tamae, Okazaki, Yuichi, Itoh, Chie, Noda, Keisuke, Tajima, Manami, Satoh, Misako, Goto, Makoto, Mitsui, Youji, Tahara, Hidetoshi, Ide, Toshinori, Furuichi, Yasuhiro, and Sugimoto, Masanobu

Subjects
EPSTEIN-Barr virus, CELL lines, TELOMERASE, CARCINOGENESIS, TUMOR suppressor genes
Abstract

We studied tumorigenic and phenotypic characteristics of pre- and postimmortal human B-lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus (EBV): preimmortal LCLs showed low telomerase activity and a normal diploid karyotype while postimmortal LCLs showed much higher telomerase activity and maintained a clonal aneuploidic state. Among five postimmortal LCLs tested, LCLs N0005 and N6803 formed colonies in agar medium and showed marked aneuploidy, and N6803 was transplantable into nude mice indicating that it had a complete malignant phenotype, but all preimmortal LCLs and the remaining three postimmortal LCLs lacked these characteristics. The products of tumor suppresser genes, p16[sup INK4A] and pRb, were downregulated in these two LCLs, and the p53 gene was mutated in N0005 LCL. We believe these results showed for the first time that some postimmortal EBV-transformed LCLs can become tumorigenic, contrary to previous reports, and that these LCLs provide an in vitro model of tumorigenesis induced by EBV. [ABSTRACT FROM AUTHOR]

Published
2003
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35. Four novel mutations of the Fanconi anemia group A gene (FAA) in Japanese patients.

Author

Nakamura, Asako, Matsuura, Shinya, Tauchi, Hiroshi, Hanada, Ryoji, Ohashi, Hirofumi, Hasegawa, Tomonobu, Honda, Koujiro, Masuno, Mitsuo, Imaizumi, Kiyoshi, Sugita, Katsuo, Ide, Toshinori, and Komatsu, K.

Subjects
FANCONI'S anemia, GENES, GENETIC mutation, GENETIC polymorphisms
Abstract

Abstract Fanconi anemia (FA) is an autosomal recessive disorder characterized by pancytopenia, predisposition to cancers, and a diverse variety of congenital malformations. At least eight complementation groups, A through H, have been described. Recently, the FA-A gene (FAA) has been isolated, and a large number of distinct mutations reported in ethnically diverse FA-A patients. Here, we report on the mutation analysis of five FA patients by single-strand conformation polymorphism. Out of five patients, at least three were found to have mutations in the FAA gene. The first patient was a compound heterozygote with a 1-bp deletion and a single-base substitution. The second patient had a heterozygous 2-bp deletion, which introduces a premature termination codon, and the third patient had a heterozygous splice donor site mutation in intron 27. [ABSTRACT FROM AUTHOR]

Published
1999
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47. G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs.

Author

Tahara, Hidetoshi, Kusunoki, Mayuko, Yamanaka, Yusuke, Matsumura, Shusaku, and Ide, Toshinori

Subjects
TELOMERES, DNA, NUCLEIC acid hybridization, MOLECULAR biology, PHYSIOLOGY
Abstract

Accurate measurement of telomeric 3′-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA. [ABSTRACT FROM AUTHOR]

Published
2005
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