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Start Over Author "Holly Huang" Publication Type Academic Journals
2 results on '"Holly Huang"'

1. Structure and stability changes of human IgG1 Fc as a consequence of methionine oxidation

Author

Dingjiang Liu, Da Ren, Holly Huang, Dankberg, Jane, Rosenfeld, Robert, Cocco, Melanie J., Li, Luke, Brems, David N., and Remmele, Richard L., Jr.

Subjects
Escherichia coli -- Physiological aspects, Escherichia coli -- Genetic aspects, Immunoglobulins -- Chemical properties, Methionine -- Chemical properties, Oxidation-reduction reaction -- Analysis, Biological sciences, Chemistry
Abstract

Biophysical and bioanalytical methods were used to characterize the fully oxidized Fc and understand the effect of methionine oxidation on the structure and stability of the human IgG1 Fc expressed in Escherichia coli. The results revealed the need to monitor and control methionine oxidation during manufacturing and storage of protein therapeutics to minimize its impact on the Fc's physical and covalent stability and potentially its biological functions.

Published
2008

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