1,655 results on '"Hof, Patrick R"'
Search Results
2. Evolutionary scaling and cognitive correlates of primate frontal cortex microstructure
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Stimpson, Cheryl D., Smaers, Jeroen B., Raghanti, Mary Ann, Phillips, Kimberley A., Jacobs, Bob, Hopkins, William D., Hof, Patrick R., and Sherwood, Chet C.
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- 2024
- Full Text
- View/download PDF
3. Deep learning-based localization algorithms on fluorescence human brain 3D reconstruction: a comparative study using stereology as a reference
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Checcucci, Curzio, Wicinski, Bridget, Mazzamuto, Giacomo, Scardigli, Marina, Ramazzotti, Josephine, Brady, Niamh, Pavone, Francesco S., Hof, Patrick R., Costantini, Irene, and Frasconi, Paolo
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- 2024
- Full Text
- View/download PDF
4. A guide to the BRAIN Initiative Cell Census Network data ecosystem
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Hawrylycz, Michael, Martone, Maryann E, Ascoli, Giorgio A, Bjaalie, Jan G, Dong, Hong-Wei, Ghosh, Satrajit S, Gillis, Jesse, Hertzano, Ronna, Haynor, David R, Hof, Patrick R, Kim, Yongsoo, Lein, Ed, Liu, Yufeng, Miller, Jeremy A, Mitra, Partha P, Mukamel, Eran, Ng, Lydia, Osumi-Sutherland, David, Peng, Hanchuan, Ray, Patrick L, Sanchez, Raymond, Regev, Aviv, Ropelewski, Alex, Scheuermann, Richard H, Tan, Shawn Zheng Kai, Thompson, Carol L, Tickle, Timothy, Tilgner, Hagen, Varghese, Merina, Wester, Brock, White, Owen, Zeng, Hongkui, Aevermann, Brian, Allemang, David, Ament, Seth, Athey, Thomas L, Baker, Cody, Baker, Katherine S, Baker, Pamela M, Bandrowski, Anita, Banerjee, Samik, Bishwakarma, Prajal, Carr, Ambrose, Chen, Min, Choudhury, Roni, Cool, Jonah, Creasy, Heather, D’Orazi, Florence, Degatano, Kylee, Dichter, Benjamin, Ding, Song-Lin, Dolbeare, Tim, Ecker, Joseph R, Fang, Rongxin, Fillion-Robin, Jean-Christophe, Fliss, Timothy P, Gee, James, Gillespie, Tom, Gouwens, Nathan, Zhang, Guo-Qiang, Halchenko, Yaroslav O, Harris, Nomi L, Herb, Brian R, Hintiryan, Houri, Hood, Gregory, Horvath, Sam, Huo, Bingxing, Jarecka, Dorota, Jiang, Shengdian, Khajouei, Farzaneh, Kiernan, Elizabeth A, Kir, Huseyin, Kruse, Lauren, Lee, Changkyu, Lelieveldt, Boudewijn, Li, Yang, Liu, Hanqing, Liu, Lijuan, Markuhar, Anup, Mathews, James, Mathews, Kaylee L, Mezias, Chris, Miller, Michael I, Mollenkopf, Tyler, Mufti, Shoaib, Mungall, Christopher J, Orvis, Joshua, Puchades, Maja A, Qu, Lei, Receveur, Joseph P, Ren, Bing, Sjoquist, Nathan, Staats, Brian, Tward, Daniel, van Velthoven, Cindy TJ, Wang, Quanxin, Xie, Fangming, Xu, Hua, Yao, Zizhen, and Yun, Zhixi
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Biological Sciences ,Genetics ,Data Science ,Neurosciences ,Mental Health ,1.1 Normal biological development and functioning ,Neurological ,Animals ,Humans ,Mice ,Brain ,Ecosystem ,Neurons ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Developmental Biology ,Agricultural ,veterinary and food sciences ,Biological sciences ,Biomedical and clinical sciences - Abstract
Characterizing cellular diversity at different levels of biological organization and across data modalities is a prerequisite to understanding the function of cell types in the brain. Classification of neurons is also essential to manipulate cell types in controlled ways and to understand their variation and vulnerability in brain disorders. The BRAIN Initiative Cell Census Network (BICCN) is an integrated network of data-generating centers, data archives, and data standards developers, with the goal of systematic multimodal brain cell type profiling and characterization. Emphasis of the BICCN is on the whole mouse brain with demonstration of prototype feasibility for human and nonhuman primate (NHP) brains. Here, we provide a guide to the cellular and spatial approaches employed by the BICCN, and to accessing and using these data and extensive resources, including the BRAIN Cell Data Center (BCDC), which serves to manage and integrate data across the ecosystem. We illustrate the power of the BICCN data ecosystem through vignettes highlighting several BICCN analysis and visualization tools. Finally, we present emerging standards that have been developed or adopted toward Findable, Accessible, Interoperable, and Reusable (FAIR) neuroscience. The combined BICCN ecosystem provides a comprehensive resource for the exploration and analysis of cell types in the brain.
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- 2023
5. Differential Vulnerability of Hippocampal Subfields in Primary Age-Related Tauopathy and Chronic Traumatic Encephalopathy.
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Farrell, Kurt, Iida, Megan A, Cherry, Jonathan D, Casella, Alicia, Stein, Thor D, Bieniek, Kevin F, Walker, Jamie M, Richardson, Timothy E, White, Charles L, Alvarez, Victor E, Huber, Bertrand R, Dickson, Dennis W, Insausti, Ricardo, Dams-O'Connor, Kristen, Vonsattel, Jean-Paul, Teich, Andy F, Gearing, Marla, Glass, Jonathan, Troncoso, Juan C, Frosch, Matthew P, Hyman, Bradley T, Murray, Melissa E, Attems, Johannes, Flanagan, Margaret E, Mao, Qinwen, Mesulam, M-Marsel, Weintraub, Sandra, Woltjer, Randy L, Pham, Thao, Kofler, Julia, Schneider, Julie A, Yu, Lei, Purohit, Dushyant P, Haroutunian, Vahram, Hof, Patrick R, Gandy, Sam, Sano, Mary, Beach, Thomas G, Poon, Wayne, Kawas, Claudia H, Corrada, María M, Rissman, Robert A, Metcalf, Jeff, Shuldberg, Sara, Salehi, Bahar, Nelson, Peter T, Trojanowski, John Q, Lee, Edward B, Wolk, David A, McMillan, Corey T, Keene, C Dirk, Latimer, Caitlin S, Montine, Thomas J, Kovacs, Gabor G, Lutz, Mirjam I, Fischer, Peter, Perrin, Richard J, Cairns, Nigel J, McKee, Ann C, and Crary, John F
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Biomedical and Clinical Sciences ,Neurosciences ,Clinical Sciences ,Frontotemporal Dementia (FTD) ,Acquired Cognitive Impairment ,Aging ,Brain Disorders ,Dementia ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Alzheimer's Disease ,Neurodegenerative ,Aetiology ,2.1 Biological and endogenous factors ,Chronic Traumatic Encephalopathy ,Hippocampus ,Humans ,Neurofibrillary Tangles ,Tauopathies ,tau Proteins ,Chronic traumatic encephalopathy ,Primary age-related tauopathy ,Repetitive head impacts ,Tauopathy ,Part Working Group ,Neurology & Neurosurgery ,Clinical sciences - Abstract
Chronic traumatic encephalopathy (CTE) is a tauopathy associated with repetitive mild head impacts characterized by perivascular hyperphosphorylated tau (p-tau) in neurofibrillary tangles (NFTs) and neurites in the depths of the neocortical sulci. In moderate to advanced CTE, NFTs accumulate in the hippocampus, potentially overlapping neuroanatomically with primary age-related tauopathy (PART), an age-related tauopathy characterized by Alzheimer disease-like tau pathology in the hippocampus devoid of amyloid plaques. We measured p-tau burden using positive-pixel counts on immunohistochemically stained and neuroanatomically segmented hippocampal tissue. Subjects with CTE had a higher total p-tau burden than PART subjects in all sectors (p = 0.005). Within groups, PART had significantly higher total p-tau burden in CA1/subiculum compared to CA3 (p = 0.02) and CA4 (p = 0.01) and total p-tau burden in CA2 trended higher than CA4 (p = 0.06). In CTE, total p-tau burden in CA1/subiculum was significantly higher than in the dentate gyrus; and CA2 also trended higher than dentate gyrus (p = 0.01, p = 0.06). When controlling for p-tau burden across the entire hippocampus, CA3 and CA4 had significantly higher p-tau burden in CTE than PART (p
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- 2022
6. Neuronal TIMP2 regulates hippocampus-dependent plasticity and extracellular matrix complexity
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Ferreira, Ana Catarina, Hemmer, Brittany M., Philippi, Sarah M., Grau-Perales, Alejandro B., Rosenstadt, Jacob L., Liu, Hanxiao, Zhu, Jeffrey D., Kareva, Tatyana, Ahfeldt, Tim, Varghese, Merina, Hof, Patrick R., and Castellano, Joseph M.
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- 2023
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7. Reduced cortical neuron number and neuron density in schizophrenia with focus on area 24: a post-mortem case–control study
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Gaus, Richard, Popal, Melanie, Heinsen, Helmut, Schmitt, Andrea, Falkai, Peter, Hof, Patrick R., Schmitz, Christoph, and Vollhardt, Alisa
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- 2023
- Full Text
- View/download PDF
8. Late chronic local inflammation, synaptic alterations, vascular remodeling and arteriovenous malformations in the brains of male rats exposed to repetitive low-level blast overpressures
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Gama Sosa, Miguel A., De Gasperi, Rita, Pryor, Dylan, Perez Garcia, Georgina S., Perez, Gissel M., Abutarboush, Rania, Kawoos, Usmah, Hogg, Seth, Ache, Benjamin, Sowa, Allison, Tetreault, Timothy, Varghese, Merina, Cook, David G., Zhu, Carolyn W., Tappan, Susan J., Janssen, William G. M., Hof, Patrick R., Ahlers, Stephen T., and Elder, Gregory A.
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- 2023
- Full Text
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9. Hispano‐American Brain Bank on Neurodevelopmental Disorders: An initiative to promote brain banking, research, education, and outreach in the field of neurodevelopmental disorders
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Dufour, Brett D, Albores‐Gallo, Lilia, Luna‐Muñoz, Jose, Hagerman, Randi, Miquelajauregui, Amaya, Buriticá, Efrain, Saldarriaga, Wilmar, Pacheco‐Herrero, Mar, Silvestre‐Sosa, Ana Yris, Mazefsky, Carla, Gastgeb, Holly, Kofler, Julia, Casanova, Manuel, Hof, Patrick R, London, Eric, Hagerman, Paul, and Martínez‐Cerdeño, Verónica
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Biomedical and Clinical Sciences ,Neurosciences ,Clinical Sciences ,Behavioral and Social Science ,Autism ,Mental Health ,Fragile X Syndrome ,Brain Disorders ,Pediatric ,Rare Diseases ,Intellectual and Developmental Disabilities (IDD) ,2.1 Biological and endogenous factors ,Aetiology ,Mental health ,Neurological ,Autism Spectrum Disorder ,Autistic Disorder ,Brain ,Humans ,Neurodevelopmental Disorders ,autism ,brain bank ,Down syndrome ,Fragile X syndrome ,Latinoamerica ,neurodevelopmental disorders ,Neurology & Neurosurgery ,Clinical sciences - Abstract
Neurodevelopmental disorders (NDDs) are conditions that present with brain dysfunction due to alterations in the processes of brain development. They present with neuropsychiatric, cognitive, and motor symptoms. Autism spectrum disorder (ASD) and Fragile X syndrome (FXS) are two of the most common NDDs. Human brain tissue is a scarce resource that is obtained from postmortem donations. In the case of NDDs, specifically autism, the reduced donation rate of brains prevents researchers to investigate its pathology and fine anatomy. The Hispano-American Brain Bank of Neurodevelopmental Disorders (Banco Hispanoamericano de CErebros de trastornos del NEurodesarrollo) or CENE is a large-scale brain bank for neurodevelopmental disorders in Hispano-America and the US. CENE's objectives are to collect and distribute brains of patients with NDDS, with a focus on ASD and FXS, to perform research, promote education of future scientists, and enhance public awareness about the importance of human tissue availability for scientific research on brain function and disease. CENE has thus far established a bilingual system of nodes and teams in several American countries including California-US, Pennsylvania-US, México, Puerto Rico, Colombia, and Dominican Republic. CENE ensures that postmortem NDD samples used in research better match the world's genetic and ethnic diversity. CENE enables and expands NDD brain research worldwide, particularly with respect to ASD and FXS.
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- 2022
10. Demographic, clinical, biomarker, and neuropathological correlates of posterior cortical atrophy: an international cohort study and individual participant data meta-analysis
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Abdi, Zeinab, Agosta, Federica, Ahmed, Samrah, Alcolea, Daniel, Allen, Isabel Elaine, Allinson, Kieren S.J., Apostolova, Liana G., Arighi, Andrea, Balasa, Mircea, Barkhof, Frederik, Best, John, Boon, Baayla D., Brandt, Katherine D., Brosch, Jared, Burrell, James, Butler, Christopher R., Calandri, Ismael, Caminiti, Silvia Paola, Canu, Elisa, Carrillo, Maria C., Caso, Francesca, Chapleau, Marianne, Chrem Mendez, Patricio, Chu, Min, Crutch, Sebastian, Cordato, Nicholas, Costa, Ana Sofia, Cui, Yue, Dickerson, Bradford, Dickson, Dennis W., Duara, Ranjan, Dubois, Bruno, Eldaief, Mark, Farlow, Martin, Fenoglio, Chiara, Filippi, Massimo, Fliessbach, Klaus, Formaglio, Maïté, Fortea, Juan, Fox, Nick, Foxe, David, Tilikete, Caroline Froment, Frosch, Matthew P., Fumagalli, Giorgio Giulio, Galasko, Douglas, Galimberti, Daniela, Garat, Oscar, Giardinieri, Giulia, Graff-Radford, Jonathan, Graff-Radford, Neill R., Grinberg, Lea, Groot, Colin, Hake, Ann Marie, Hansson, Oskar, Headley, Alison, Hernandez, Micaela, Hochberg, Daisy, Hodges, John R., Hof, Patrick R., Holton, Janice, Hromas, Gabrielle, Gala, Ignacio Illán, Irwin, David J., Jaunmuktane, Zane, Jing, Donglai, Josephs, Keith, Kagerer, Sonja M., Kasuga, Kensaku, Kong, Yu, Kövari, Enikö, Lacombe-Thibault, Mégane, Lleó, Alberto, Laforce, Robert, La Joie, Renaud, Lashley, Tammaryn, Leger, Gabriel, Levin, Netta, Levy, Richard, Liu, Yang, Liu, Li, Lladó Plarrumaní, Albert, Lucente, Diane E., Machulda, Mary M., Magnani, Giuseppe, Magnin, Eloi, Malpetti, Maura, Matthews, Brandy, McGinnis, Scott, Mendez, Mario F., Mesulam, Marsel, Migliaccio, Raffaella, Miklitz, Carolin, Miller, Zachary A., Montembeault, Maxime, Murray, Melissa E., Mundada, Nidhi, Nemes, Sara, Nestor, Peter J., Ocal, Dilek, Ossenkoppele, Rik, Paterson, Ross, Pelak, Victoria, Perani, Daniela, Phillips, Jeffrey, Piguet, Olivier, Pijnenburg, Yolande, Putcha, Deepti, Quimby, Megan, Rabinovici, Gil D., Reetz, Kathrin, Rein, Netaniel, Revesz, Tamas, Rezaii, Neguine, Rodriguez-Porcel, Federico, Rogalski, Emily, Rowe, James B., Ryan, Natalie, Sanchez-Valle, Raquel, Sacchi, Luca, Santos-Santos, Miguel Ángel, Schott, Jonathan M., Seeley, William, Sherman, Janet, Spina, Salvatore, Stomrud, Erik, Sullivan, A. Campbell, Tanner, Jeremy, Tideman, Pontus, Tokutake, Takayoshi, Tondo, Giacomo, Touroutoglou, Alexandra, Tousi, Babak, Vandenberghe, Rik, van der Flier, Wiesje, Walker, Jamie M., Weintraub, Sandra, Whitwell, Jennifer L., Wolk, David A., Wong, Bonnie, Wu, Liyong, Xie, Kexin, Yong, Keir, Apostolova, Liana, Boon, Baayla D C, Grinberg, Lea T, Irwin, David J, Josephs, Keith A, Mendez, Mario F, Mendez, Patricio Chrem, Miller, Zachary A, Murray, Melissa E, Nemes, Sára, Schott, Jonathan M, Sullivan, A Campbell, Walker, Jamie, Whitwell, Jennifer L, Wolk, David A, and Rabinovici, Gil D
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- 2024
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11. Genome-wide association study and functional validation implicates JADE1 in tauopathy
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Farrell, Kurt, Kim, SoongHo, Han, Natalia, Iida, Megan A, Gonzalez, Elias M, Otero-Garcia, Marcos, Walker, Jamie M, Richardson, Timothy E, Renton, Alan E, Andrews, Shea J, Fulton-Howard, Brian, Humphrey, Jack, Vialle, Ricardo A, Bowles, Kathryn R, de Paiva Lopes, Katia, Whitney, Kristen, Dangoor, Diana K, Walsh, Hadley, Marcora, Edoardo, Hefti, Marco M, Casella, Alicia, Sissoko, Cheick T, Kapoor, Manav, Novikova, Gloriia, Udine, Evan, Wong, Garrett, Tang, Weijing, Bhangale, Tushar, Hunkapiller, Julie, Ayalon, Gai, Graham, Robert R, Cherry, Jonathan D, Cortes, Etty P, Borukov, Valeriy Y, McKee, Ann C, Stein, Thor D, Vonsattel, Jean-Paul, Teich, Andy F, Gearing, Marla, Glass, Jonathan, Troncoso, Juan C, Frosch, Matthew P, Hyman, Bradley T, Dickson, Dennis W, Murray, Melissa E, Attems, Johannes, Flanagan, Margaret E, Mao, Qinwen, Mesulam, M-Marsel, Weintraub, Sandra, Woltjer, Randy L, Pham, Thao, Kofler, Julia, Schneider, Julie A, Yu, Lei, Purohit, Dushyant P, Haroutunian, Vahram, Hof, Patrick R, Gandy, Sam, Sano, Mary, Beach, Thomas G, Poon, Wayne, Kawas, Claudia H, Corrada, María M, Rissman, Robert A, Metcalf, Jeff, Shuldberg, Sara, Salehi, Bahar, Nelson, Peter T, Trojanowski, John Q, Lee, Edward B, Wolk, David A, McMillan, Corey T, Keene, C Dirk, Latimer, Caitlin S, Montine, Thomas J, Kovacs, Gabor G, Lutz, Mirjam I, Fischer, Peter, Perrin, Richard J, Cairns, Nigel J, Franklin, Erin E, Cohen, Herbert T, Raj, Towfique, Cobos, Inma, Frost, Bess, Goate, Alison, White III, Charles L, and Crary, John F
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Biomedical and Clinical Sciences ,Neurosciences ,Neurodegenerative ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Brain Disorders ,Genetics ,Dementia ,Acquired Cognitive Impairment ,Frontotemporal Dementia (FTD) ,Aging ,Alzheimer's Disease ,Aetiology ,2.1 Biological and endogenous factors ,Neurological ,Aged ,Aged ,80 and over ,Animals ,Cohort Studies ,Drosophila ,Female ,Genome-Wide Association Study ,Homeodomain Proteins ,Humans ,Male ,Middle Aged ,Polymorphism ,Single Nucleotide ,Tauopathies ,Tumor Suppressor Proteins ,Clinical Sciences ,Neurology & Neurosurgery - Abstract
Primary age-related tauopathy (PART) is a neurodegenerative pathology with features distinct from but also overlapping with Alzheimer disease (AD). While both exhibit Alzheimer-type temporal lobe neurofibrillary degeneration alongside amnestic cognitive impairment, PART develops independently of amyloid-β (Aβ) plaques. The pathogenesis of PART is not known, but evidence suggests an association with genes that promote tau pathology and others that protect from Aβ toxicity. Here, we performed a genetic association study in an autopsy cohort of individuals with PART (n = 647) using Braak neurofibrillary tangle stage as a quantitative trait. We found some significant associations with candidate loci associated with AD (SLC24A4, MS4A6A, HS3ST1) and progressive supranuclear palsy (MAPT and EIF2AK3). Genome-wide association analysis revealed a novel significant association with a single nucleotide polymorphism on chromosome 4 (rs56405341) in a locus containing three genes, including JADE1 which was significantly upregulated in tangle-bearing neurons by single-soma RNA-seq. Immunohistochemical studies using antisera targeting JADE1 protein revealed localization within tau aggregates in autopsy brains with four microtubule-binding domain repeats (4R) isoforms and mixed 3R/4R, but not with 3R exclusively. Co-immunoprecipitation in post-mortem human PART brain tissue revealed a specific binding of JADE1 protein to four repeat tau lacking N-terminal inserts (0N4R). Finally, knockdown of the Drosophila JADE1 homolog rhinoceros (rno) enhanced tau-induced toxicity and apoptosis in vivo in a humanized 0N4R mutant tau knock-in model, as quantified by rough eye phenotype and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) in the fly brain. Together, these findings indicate that PART has a genetic architecture that partially overlaps with AD and other tauopathies and suggests a novel role for JADE1 as a modifier of neurofibrillary degeneration.
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- 2022
12. Comparative cellular analysis of motor cortex in human, marmoset and mouse
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Bakken, Trygve E, Jorstad, Nikolas L, Hu, Qiwen, Lake, Blue B, Tian, Wei, Kalmbach, Brian E, Crow, Megan, Hodge, Rebecca D, Krienen, Fenna M, Sorensen, Staci A, Eggermont, Jeroen, Yao, Zizhen, Aevermann, Brian D, Aldridge, Andrew I, Bartlett, Anna, Bertagnolli, Darren, Casper, Tamara, Castanon, Rosa G, Crichton, Kirsten, Daigle, Tanya L, Dalley, Rachel, Dee, Nick, Dembrow, Nikolai, Diep, Dinh, Ding, Song-Lin, Dong, Weixiu, Fang, Rongxin, Fischer, Stephan, Goldman, Melissa, Goldy, Jeff, Graybuck, Lucas T, Herb, Brian R, Hou, Xiaomeng, Kancherla, Jayaram, Kroll, Matthew, Lathia, Kanan, van Lew, Baldur, Li, Yang Eric, Liu, Christine S, Liu, Hanqing, Lucero, Jacinta D, Mahurkar, Anup, McMillen, Delissa, Miller, Jeremy A, Moussa, Marmar, Nery, Joseph R, Nicovich, Philip R, Niu, Sheng-Yong, Orvis, Joshua, Osteen, Julia K, Owen, Scott, Palmer, Carter R, Pham, Thanh, Plongthongkum, Nongluk, Poirion, Olivier, Reed, Nora M, Rimorin, Christine, Rivkin, Angeline, Romanow, William J, Sedeño-Cortés, Adriana E, Siletti, Kimberly, Somasundaram, Saroja, Sulc, Josef, Tieu, Michael, Torkelson, Amy, Tung, Herman, Wang, Xinxin, Xie, Fangming, Yanny, Anna Marie, Zhang, Renee, Ament, Seth A, Behrens, M Margarita, Bravo, Hector Corrada, Chun, Jerold, Dobin, Alexander, Gillis, Jesse, Hertzano, Ronna, Hof, Patrick R, Höllt, Thomas, Horwitz, Gregory D, Keene, C Dirk, Kharchenko, Peter V, Ko, Andrew L, Lelieveldt, Boudewijn P, Luo, Chongyuan, Mukamel, Eran A, Pinto-Duarte, António, Preissl, Sebastian, Regev, Aviv, Ren, Bing, Scheuermann, Richard H, Smith, Kimberly, Spain, William J, White, Owen R, Koch, Christof, Hawrylycz, Michael, Tasic, Bosiljka, Macosko, Evan Z, McCarroll, Steven A, and Ting, Jonathan T
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Human Genome ,Neurosciences ,Genetics ,Biotechnology ,Underpinning research ,1.1 Normal biological development and functioning ,Neurological ,Animals ,Atlases as Topic ,Callithrix ,Epigenesis ,Genetic ,Epigenomics ,Female ,GABAergic Neurons ,Gene Expression Profiling ,Glutamates ,Humans ,In Situ Hybridization ,Fluorescence ,Male ,Mice ,Middle Aged ,Motor Cortex ,Neurons ,Organ Specificity ,Phylogeny ,Single-Cell Analysis ,Species Specificity ,Transcriptome ,General Science & Technology - Abstract
The primary motor cortex (M1) is essential for voluntary fine-motor control and is functionally conserved across mammals1. Here, using high-throughput transcriptomic and epigenomic profiling of more than 450,000 single nuclei in humans, marmoset monkeys and mice, we demonstrate a broadly conserved cellular makeup of this region, with similarities that mirror evolutionary distance and are consistent between the transcriptome and epigenome. The core conserved molecular identities of neuronal and non-neuronal cell types allow us to generate a cross-species consensus classification of cell types, and to infer conserved properties of cell types across species. Despite the overall conservation, however, many species-dependent specializations are apparent, including differences in cell-type proportions, gene expression, DNA methylation and chromatin state. Few cell-type marker genes are conserved across species, revealing a short list of candidate genes and regulatory mechanisms that are responsible for conserved features of homologous cell types, such as the GABAergic chandelier cells. This consensus transcriptomic classification allows us to use patch-seq (a combination of whole-cell patch-clamp recordings, RNA sequencing and morphological characterization) to identify corticospinal Betz cells from layer 5 in non-human primates and humans, and to characterize their highly specialized physiology and anatomy. These findings highlight the robust molecular underpinnings of cell-type diversity in M1 across mammals, and point to the genes and regulatory pathways responsible for the functional identity of cell types and their species-specific adaptations.
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- 2021
13. Genome-wide association study and functional validation implicates JADE1 in tauopathy
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Farrell, Kurt, Kim, SoongHo, Han, Natalia, Iida, Megan A, Gonzalez, Elias, Otero-Garcia, Marcos, Walker, Jamie, Richardson, Tim, Renton, Alan E, Andrews, Shea J, Fulton-Howard, Brian, Humphrey, Jack, Vialle, Ricardo A, Bowles, Kathryn R, Whitney, Kristen, Dangoor, Diana K, Marcora, Edoardo, Hefti, Marco M, Casella, Alicia, Sissoko, Cheick, Kapoor, Manav, Novikova, Gloriia, Udine, Evan, Wong, Garrett, Tang, Weijing, Bhangale, Tushar, Hunkapiller, Julie, Ayalon, Gai, Graham, Rob, Cherry, Jonathan D, Cortes, Etty, Borukov, Valeriy, McKee, Ann C, Stein, Thor D, Vonsattel, Jean-Paul, Teich, Andy F, Gearing, Marla, Glass, Jonathan, Troncoso, Juan C, Frosch, Matthew P, Hyman, Bradley T, Dickson, Dennis W, Murray, Melissa E, Attems, Johannes, Flanagan, Margaret E, Mao, Qinwen, Mesulam, M-Marsel, Weintraub, Sandra, Woltjer, Randy, Pham, Thao, Kofler, Julia, Schneider, Julie A, Yu, Lei, Purohit, Dushyant P, Haroutunian, Vahram, Hof, Patrick R, Gandy, Sam, Sano, Mary, Beach, Thomas G, Poon, Wayne, Kawas, Claudia, Corrada, María, Rissman, Robert A, Metcalf, Jeff, Shuldberg, Sara, Salehi, Bahar, Nelson, Peter T, Trojanowski, John Q, Lee, Edward B, Wolk, David A, McMillan, Corey T, Keene, Dirk C, Montine, Thomas J, Kovacs, Gabor G, Lutz, Mirjam I, Fischer, Peter, Perrin, Richard J, Cairns, Nigel, Franklin, Erin E, Cohen, Herbert T, Sillero, Maria Inmaculada Cobos, Frost, Bess, Raj, Towfique, Goate, Alison, White, Charles L, and Crary, John F
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Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Genetics ,Dementia ,Alzheimer's Disease ,Frontotemporal Dementia (FTD) ,Brain Disorders ,Neurosciences ,Biotechnology ,Neurodegenerative ,Aging ,Acquired Cognitive Impairment ,Aetiology ,2.1 Biological and endogenous factors ,Neurological - Abstract
AbstractPrimary age-related tauopathy (PART) is a neurodegenerative tauopathy with features distinct from but also overlapping with Alzheimer disease (AD). While both exhibit Alzheimer-type temporal lobe neurofibrillary degeneration alongside amnestic cognitive impairment, PART develops independently of amyloid-β (Aβ) deposition in plaques. The pathogenesis of PART is unknown, but evidence suggests it is associated with genes that promote tau pathology as well as others that protect from Aβ toxicity. Here, we performed a genetic association study in an autopsy cohort of individuals with PART (n=647) using Braak neurofibrillary tangle stage as a quantitative trait adjusting for sex, age, genotyping platform, and principal components. We found significant associations with some candidate loci associated with AD and progressive supranuclear palsy, a primary tauopathy (SLC24A4, MS4A6A, HS3ST1, MAPT and EIF2AK3). Genome-wide association analysis revealed a novel significant association with a single nucleotide polymorphism on chromosome 4 (rs56405341) in a locus containing three genes, including JADE1 which was significantly upregulated in tangle-bearing neurons by single-soma RNA-seq. Immunohistochemical studies using antisera targeting JADE1 protein revealed localization within tau aggregates in autopsy brain from tauopathies containing isoforms with four microtubule-binding domain repeats (4R) and mixed 3R/4R, but not with 3R exclusively. Co-immunoprecipitation revealed a direct and specific binding of JADE1 protein to tau containing four (4R) and no N-terminal inserts (0N4R) in post-mortem human PART brain tissue. Finally, knockdown of the Drosophila JADE1 homolog rhinoceros (rno) enhanced tau-induced toxicity and apoptosis in vivo in a humanized 0N4R mutant tau knock-in model as quantified by rough eye phenotype and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) in the fly brain. Together, these findings indicate that PART has a genetic architecture that partially overlaps with AD and other tauopathies and suggests a novel role for JADE1 as a mediator of neurofibrillary degeneration.
- Published
- 2021
14. Early Selective Vulnerability of the CA2 Hippocampal Subfield in Primary Age-Related Tauopathy (vol 80, nlaa153, 2021)
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Walker, Jamie M, Richardson, Timothy E, Farrell, Kurt, Iida, Megan A, Foong, Chan, Shang, Ping, Attems, Johannes, Ayalon, Gai, Beach, Thomas G, Bigio, Eileen H, Budson, Andrew, Cairns, Nigel J, Corrada, Maria, Cortes, Etty, Dickson, Dennis W, Fischer, Peter, Flanagan, Margaret E, Franklin, Erin, Gearing, Marla, Glass, Jonathan, Hansen, Lawrence A, Haroutunian, Vahram, Hof, Patrick R, Honig, Lawrence, Kawas, Claudia, Keene, C Dirk, Kofler, Julia, Kovacs, Gabor G, Lee, Edward B, Lutz, Mirjam I, Mao, Qinwen, Masliah, Eliezer, McKee, Ann C, McMillan, Corey T, Mesulam, M Marsel, Murray, Melissa, Nelson, Peter T, Perrin, Richard, Pham, Thao, Poon, Wayne, Dushyant, P Purohit, Rissman, Robert A, Sakai, Kenji, Sano, Mary, Schneider, Julie A, Stein, Thor D, Teich, Andrew F, Trojanowski, John Q, Troncoso, Juan C, Vonsattel, Jean-Paul, Weintraub, Sandra, Wolk, David A, Woltjer, Randall L, Yamada, Masahito, Yu, Lei, White, Charles L, and Crary, John F
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Biomedical and Clinical Sciences ,Neurosciences ,Clinical Sciences ,Neurology & Neurosurgery ,Clinical sciences - Published
- 2021
15. Single basolateral amygdala neurons in macaques exhibit distinct connectional motifs with frontal cortex
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Zeisler, Zachary R., London, Liza, Janssen, William G., Fredericks, J. Megan, Elorette, Catherine, Fujimoto, Atsushi, Zhan, Huiqing, Russ, Brian E., Clem, Roger L., Hof, Patrick R., Stoll, Frederic M., and Rudebeck, Peter H.
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- 2023
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16. Cortical Interlaminar Astrocytes Are Generated Prenatally, Mature Postnatally, and Express Unique Markers in Human and Nonhuman Primates
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Falcone, Carmen, Penna, Elisa, Hong, Tiffany, Tarantal, Alice F, Hof, Patrick R, Hopkins, William D, Sherwood, Chet C, Noctor, Stephen C, and Martínez-Cerdeño, Verónica
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Biomedical and Clinical Sciences ,Biological Psychology ,Cognitive and Computational Psychology ,Neurosciences ,Psychology ,Stem Cell Research - Nonembryonic - Non-Human ,Stem Cell Research ,Pediatric ,Animals ,Astrocytes ,Biomarkers ,Cerebral Cortex ,Glial Fibrillary Acidic Protein ,Humans ,Macaca mulatta ,Mice ,Neurons ,astrocytes ,astrogenesis ,cerebral cortex ,development ,primates ,Cognitive Sciences ,Experimental Psychology ,Biological psychology ,Cognitive and computational psychology - Abstract
Interlaminar astrocytes (ILAs) are a subset of cortical astrocytes that reside in layer I, express GFAP, have a soma contacting the pia, and contain long interlaminar processes that extend through several cortical layers. We studied the prenatal and postnatal development of ILAs in three species of primates (rhesus macaque, chimpanzee, and human). We found that ILAs are generated prenatally likely from radial glial (RG) cells, that ILAs proliferate locally during gestation, and that ILAs extend interlaminar processes during postnatal stages of development. We showed that the density and morphological complexity of ILAs increase with age, and that ILAs express multiple markers that are expressed by RG cells (Pax6, Sox2, and Nestin), specific to inner and outer RG cells (Cryab and Hopx), and astrocyte markers (S100β, Aqp4, and GLAST) in prenatal stages and in adult. Finally, we demonstrated that rudimentary ILAs in mouse also express the RG markers Pax6, Sox2, and Nestin, but do not express S100β, Cryab, or Hopx, and that the density and morphological complexity of ILAs differ between primate species and mouse. Together these findings contribute new information on astrogenesis of this unique class of cells and suggest a lineal relationship between RG cells and ILAs.
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- 2021
17. Evolution of regulatory signatures in primate cortical neurons at cell-type resolution.
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Kozlenkov, Alexey, Vermunt, Marit W, Apontes, Pasha, Li, Junhao, Hao, Ke, Sherwood, Chet C, Hof, Patrick R, Ely, John J, Wegner, Michael, Mukamel, Eran A, Creyghton, Menno P, Koonin, Eugene V, and Dracheva, Stella
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GABAergic neurons ,H3K27ac histone modification ,glutamatergic neurons ,primate evolution ,regulatory elements - Abstract
The human cerebral cortex contains many cell types that likely underwent independent functional changes during evolution. However, cell-type-specific regulatory landscapes in the cortex remain largely unexplored. Here we report epigenomic and transcriptomic analyses of the two main cortical neuronal subtypes, glutamatergic projection neurons and GABAergic interneurons, in human, chimpanzee, and rhesus macaque. Using genome-wide profiling of the H3K27ac histone modification, we identify neuron-subtype-specific regulatory elements that previously went undetected in bulk brain tissue samples. Human-specific regulatory changes are uncovered in multiple genes, including those associated with language, autism spectrum disorder, and drug addiction. We observe preferential evolutionary divergence in neuron subtype-specific regulatory elements and show that a substantial fraction of pan-neuronal regulatory elements undergoes subtype-specific evolutionary changes. This study sheds light on the interplay between regulatory evolution and cell-type-dependent gene-expression programs, and provides a resource for further exploration of human brain evolution and function.
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- 2020
18. Neuronal vulnerability to brain aging and neurodegeneration in cognitively impaired marmoset monkeys (Callithrix jacchus)
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Freire-Cobo, Carmen, Rothwell, Emily S., Varghese, Merina, Edwards, Mélise, Janssen, William G.M., Lacreuse, Agnès, and Hof, Patrick R.
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- 2023
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19. Evidence of traumatic brain injury in headbutting bovids
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Ackermans, Nicole L., Varghese, Merina, Williams, Terrie M., Grimaldi, Nicholas, Selmanovic, Enna, Alipour, Akbar, Balchandani, Priti, Reidenberg, Joy S., and Hof, Patrick R.
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- 2022
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20. Ventral tegmental area integrity measured with high-resolution 7-Tesla MRI relates to motivation across depression and anxiety diagnoses
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Morris, Laurel S., Mehta, Marishka, Ahn, Christopher, Corniquel, Morgan, Verma, Gaurav, Delman, Bradley, Hof, Patrick R., Jacob, Yael, Balchandani, Priti, and Murrough, James W.
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- 2022
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21. Cortical interlaminar astrocytes across the therian mammal radiation
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Falcone, Carmen, Wolf‐Ochoa, Marisol, Amina, Sarwat, Hong, Tiffany, Vakilzadeh, Gelareh, Hopkins, William D, Hof, Patrick R, Sherwood, Chet C, Manger, Paul R, Noctor, Stephen C, and Martínez‐Cerdeño, Verónica
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Zoology ,Biomedical and Clinical Sciences ,Neurosciences ,Biological Sciences ,Rare Diseases ,Neurological ,Animals ,Astrocytes ,Cerebral Cortex ,Mammals ,astrocyte ,cerebral cortex ,evolution ,human ,mammal ,primate ,RRID:AB_10013382 ,RRID:AB_2057371 ,RRID:AB_91530 ,RRID:SCR_001775 ,RRID:SCR_002285 ,RRID:AB_477010 ,RRID:AB_839504 ,RRID:AB_443209 ,RRID:AB_776174 ,RRID:AB_2298772 ,RRID:AB_570666 ,RRID:AB_882426 ,RRID:AB_778021 ,RRID:AB_141708 ,RRID:AB_141633 ,RRID:AB_2313663 ,RRID:AB_2313574 ,RRID:AB_2340593 ,Medical Physiology ,Neurology & Neurosurgery - Abstract
Interlaminar astrocytes (ILA) in the cerebral cortex possess a soma in layer I and extend an interlaminar process that runs perpendicular to the pia into deeper cortical layers. We examined cerebral cortex from 46 species that encompassed most orders of therian mammalians, including 22 primate species. We described two distinct cell types with interlaminar processes that have been referred to as ILA, that we termed pial ILA and supial ILA. ILA subtypes differ in somatic morphology, position in layer I, and presence across species. We further described rudimentary ILA that have short GFAP+ processes that do not exit layer I, and "typical" ILA with longer GFAP+ processes that exit layer I. Pial ILA were present in all mammalian species analyzed, with typical ILA observed in Primates, Scandentia, Chiroptera, Carnivora, Artiodactyla, Hyracoidea, and Proboscidea. Subpial ILA were absent in Marsupialia, and typical subpial ILA were only found in Primate. We focused on the properties of pial ILA by investigating the molecular properties of pial ILA and confirming their astrocytic nature. We found that while the density of pial ILA somata only varied slightly, the complexity of ILA processes varied greatly across species. Primates, specifically bonobo, chimpanzee, orangutan, and human, exhibited pial ILA with the highest complexity. We showed that interlaminar processes contact neurons, pia, and capillaries, suggesting a potential role for ILA in the blood-brain barrier and facilitating communication among cortical neurons, astrocytes, capillaries, meninges, and cerebrospinal fluid.
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- 2019
22. Transcriptome-wide isoform-level dysregulation in ASD, schizophrenia, and bipolar disorder
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Gandal, Michael J, Zhang, Pan, Hadjimichael, Evi, Walker, Rebecca L, Chen, Chao, Liu, Shuang, Won, Hyejung, van Bakel, Harm, Varghese, Merina, Wang, Yongjun, Shieh, Annie W, Haney, Jillian, Parhami, Sepideh, Belmont, Judson, Kim, Minsoo, Moran Losada, Patricia, Khan, Zenab, Mleczko, Justyna, Xia, Yan, Dai, Rujia, Wang, Daifeng, Yang, Yucheng T, Xu, Min, Fish, Kenneth, Hof, Patrick R, Warrell, Jonathan, Fitzgerald, Dominic, White, Kevin, Jaffe, Andrew E, Peters, Mette A, Gerstein, Mark, Liu, Chunyu, Iakoucheva, Lilia M, Pinto, Dalila, Geschwind, Daniel H, Ashley-Koch, Allison E, Crawford, Gregory E, Garrett, Melanie E, Song, Lingyun, Safi, Alexias, Johnson, Graham D, Wray, Gregory A, Reddy, Timothy E, Goes, Fernando S, Zandi, Peter, Bryois, Julien, Price, Amanda J, Ivanov, Nikolay A, Collado-Torres, Leonardo, Hyde, Thomas M, Burke, Emily E, Kleiman, Joel E, Tao, Ran, Shin, Joo Heon, Akbarian, Schahram, Girdhar, Kiran, Jiang, Yan, Kundakovic, Marija, Brown, Leanne, Kassim, Bibi S, Park, Royce B, Wiseman, Jennifer R, Zharovsky, Elizabeth, Jacobov, Rivka, Devillers, Olivia, Flatow, Elie, Hoffman, Gabriel E, Lipska, Barbara K, Lewis, David A, Haroutunian, Vahram, Hahn, Chang-Gyu, Charney, Alexander W, Dracheva, Stella, Kozlenkov, Alexey, DelValle, Diane, Francoeur, Nancy, Roussos, Panos, Fullard, John F, Bendl, Jaroslav, Hauberg, Mads E, Mangravite, Lara M, Chae, Yooree, Peng, Junmin, Niu, Mingming, Wang, Xusheng, Webster, Maree J, Beach, Thomas G, Jiang, Yi, and Grennan, Kay S
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Pharmacology and Pharmaceutical Sciences ,Biological Sciences ,Biomedical and Clinical Sciences ,Genetics ,Neurosciences ,Intellectual and Developmental Disabilities (IDD) ,Brain Disorders ,Mental Illness ,Bipolar Disorder ,Mental Health ,Schizophrenia ,Biotechnology ,Serious Mental Illness ,Autism ,Human Genome ,Aetiology ,2.1 Biological and endogenous factors ,Mental health ,Good Health and Well Being ,Autism Spectrum Disorder ,Brain ,Genetic Predisposition to Disease ,Humans ,Protein Isoforms ,RNA Splicing ,Sequence Analysis ,RNA ,Transcriptome ,PsychENCODE Consortium ,General Science & Technology - Abstract
Most genetic risk for psychiatric disease lies in regulatory regions, implicating pathogenic dysregulation of gene expression and splicing. However, comprehensive assessments of transcriptomic organization in diseased brains are limited. In this work, we integrated genotypes and RNA sequencing in brain samples from 1695 individuals with autism spectrum disorder (ASD), schizophrenia, and bipolar disorder, as well as controls. More than 25% of the transcriptome exhibits differential splicing or expression, with isoform-level changes capturing the largest disease effects and genetic enrichments. Coexpression networks isolate disease-specific neuronal alterations, as well as microglial, astrocyte, and interferon-response modules defining previously unidentified neural-immune mechanisms. We integrated genetic and genomic data to perform a transcriptome-wide association study, prioritizing disease loci likely mediated by cis effects on brain expression. This transcriptome-wide characterization of the molecular pathology across three major psychiatric disorders provides a comprehensive resource for mechanistic insight and therapeutic development.
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- 2018
23. 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy
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Pesce, Luca, Scardigli, Marina, Gavryusev, Vladislav, Laurino, Annunziatina, Mazzamuto, Giacomo, Brady, Niamh, Sancataldo, Giuseppe, Silvestri, Ludovico, Destrieux, Christophe, Hof, Patrick R., Costantini, Irene, and Pavone, Francesco S.
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- 2022
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24. Segmentation of supragranular and infragranular layers in ultra-high-resolution 7T ex vivo MRI of the human cerebral cortex.
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Zeng, Xiangrui, Puonti, Oula, Sayeed, Areej, Herisse, Rogeny, Mora, Jocelyn, Evancic, Kathryn, Varadarajan, Divya, Balbastre, Yael, Costantini, Irene, Scardigli, Marina, Ramazzotti, Josephine, DiMeo, Danila, Mazzamuto, Giacomo, Pesce, Luca, Brady, Niamh, Cheli, Franco, Pavone, Francesco Saverio, Hof, Patrick R, Frost, Robert, and Augustinack, Jean
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- 2024
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25. Neuron loss associated with age but not Alzheimer's disease pathology in the chimpanzee brain
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Edler, Melissa K., Munger, Emily L., Meindl, Richard S., Hopkins, William D., Ely, John J., Erwin, Joseph M., Mufson, Elliott J., Hof, Patrick R., Sherwood, Chet C., and Raghant, Mary Ann
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- 2020
26. Molecular and cellular reorganization of neural circuits in the human lineage
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Sousa, André MM, Zhu, Ying, Raghanti, Mary Ann, Kitchen, Robert R, Onorati, Marco, Tebbenkamp, Andrew TN, Stutz, Bernardo, Meyer, Kyle A, Li, Mingfeng, Kawasawa, Yuka Imamura, Liu, Fuchen, Perez, Raquel Garcia, Mele, Marta, Carvalho, Tiago, Skarica, Mario, Gulden, Forrest O, Pletikos, Mihovil, Shibata, Akemi, Stephenson, Alexa R, Edler, Melissa K, Ely, John J, Elsworth, John D, Horvath, Tamas L, Hof, Patrick R, Hyde, Thomas M, Kleinman, Joel E, Weinberger, Daniel R, Reimers, Mark, Lifton, Richard P, Mane, Shrikant M, Noonan, James P, State, Matthew W, Lein, Ed S, Knowles, James A, Marques-Bonet, Tomas, Sherwood, Chet C, Gerstein, Mark B, and Sestan, Nenad
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Biological Sciences ,Bioinformatics and Computational Biology ,Genetics ,Neurosciences ,Brain Disorders ,1.1 Normal biological development and functioning ,Underpinning research ,Neurological ,Animals ,Gene Expression Profiling ,Humans ,Interneurons ,Macaca ,Neocortex ,Neural Pathways ,Pan troglodytes ,Phylogeny ,Species Specificity ,Transcriptome ,General Science & Technology - Abstract
To better understand the molecular and cellular differences in brain organization between human and nonhuman primates, we performed transcriptome sequencing of 16 regions of adult human, chimpanzee, and macaque brains. Integration with human single-cell transcriptomic data revealed global, regional, and cell-type-specific species expression differences in genes representing distinct functional categories. We validated and further characterized the human specificity of genes enriched in distinct cell types through histological and functional analyses, including rare subpallial-derived interneurons expressing dopamine biosynthesis genes enriched in the human striatum and absent in the nonhuman African ape neocortex. Our integrated analysis of the generated data revealed diverse molecular and cellular features of the phylogenetic reorganization of the human brain across multiple levels, with relevance for brain function and disease.
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- 2017
27. Human neocortical expansion involves glutamatergic neuron diversification
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Berg, Jim, Sorensen, Staci A., Ting, Jonathan T., Miller, Jeremy A., Chartrand, Thomas, Buchin, Anatoly, Bakken, Trygve E., Budzillo, Agata, Dee, Nick, Ding, Song-Lin, Gouwens, Nathan W., Hodge, Rebecca D., Kalmbach, Brian, Lee, Changkyu, Lee, Brian R., Alfiler, Lauren, Baker, Katherine, Barkan, Eliza, Beller, Allison, Berry, Kyla, Bertagnolli, Darren, Bickley, Kris, Bomben, Jasmine, Braun, Thomas, Brouner, Krissy, Casper, Tamara, Chong, Peter, Crichton, Kirsten, Dalley, Rachel, de Frates, Rebecca, Desta, Tsega, Lee, Samuel Dingman, D’Orazi, Florence, Dotson, Nadezhda, Egdorf, Tom, Enstrom, Rachel, Farrell, Colin, Feng, David, Fong, Olivia, Furdan, Szabina, Galakhova, Anna A., Gamlin, Clare, Gary, Amanda, Glandon, Alexandra, Goldy, Jeff, Gorham, Melissa, Goriounova, Natalia A., Gratiy, Sergey, Graybuck, Lucas, Gu, Hong, Hadley, Kristen, Hansen, Nathan, Heistek, Tim S., Henry, Alex M., Heyer, Djai B., Hill, DiJon, Hill, Chris, Hupp, Madie, Jarsky, Tim, Kebede, Sara, Keene, Lisa, Kim, Lisa, Kim, Mean-Hwan, Kroll, Matthew, Latimer, Caitlin, Levi, Boaz P., Link, Katherine E., Mallory, Matthew, Mann, Rusty, Marshall, Desiree, Maxwell, Michelle, McGraw, Medea, McMillen, Delissa, Melief, Erica, Mertens, Eline J., Mezei, Leona, Mihut, Norbert, Mok, Stephanie, Molnar, Gabor, Mukora, Alice, Ng, Lindsay, Ngo, Kiet, Nicovich, Philip R., Nyhus, Julie, Olah, Gaspar, Oldre, Aaron, Omstead, Victoria, Ozsvar, Attila, Park, Daniel, Peng, Hanchuan, Pham, Trangthanh, Pom, Christina A., Potekhina, Lydia, Rajanbabu, Ramkumar, Ransford, Shea, Reid, David, Rimorin, Christine, Ruiz, Augustin, Sandman, David, Sulc, Josef, Sunkin, Susan M., Szafer, Aaron, Szemenyei, Viktor, Thomsen, Elliot R., Tieu, Michael, Torkelson, Amy, Trinh, Jessica, Tung, Herman, Wakeman, Wayne, Waleboer, Femke, Ward, Katelyn, Wilbers, René, Williams, Grace, Yao, Zizhen, Yoon, Jae-Geun, Anastassiou, Costas, Arkhipov, Anton, Barzo, Pal, Bernard, Amy, Cobbs, Charles, de Witt Hamer, Philip C., Ellenbogen, Richard G., Esposito, Luke, Ferreira, Manuel, Gwinn, Ryder P., Hawrylycz, Michael J., Hof, Patrick R., Idema, Sander, Jones, Allan R., Keene, C. Dirk, Ko, Andrew L., Murphy, Gabe J., Ng, Lydia, Ojemann, Jeffrey G., Patel, Anoop P., Phillips, John W., Silbergeld, Daniel L., Smith, Kimberly, Tasic, Bosiljka, Yuste, Rafael, Segev, Idan, de Kock, Christiaan P. J., Mansvelder, Huibert D., Tamas, Gabor, Zeng, Hongkui, Koch, Christof, and Lein, Ed S.
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- 2021
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28. Brain and blood biomarkers of tauopathy and neuronal injury in humans and rats with neurobehavioral syndromes following blast exposure
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Dickstein, Dara L., De Gasperi, Rita, Gama Sosa, Miguel A., Perez-Garcia, Georgina, Short, Jennifer A., Sosa, Heidi, Perez, Gissel M., Tschiffely, Anna E., Dams-O’Connor, Kristen, Pullman, Mariel Y., Knesaurek, Karin, Knutsen, Andrew, Pham, Dzung L., Soleimani, Lale, Jordan, Barry D., Gordon, Wayne A., Delman, Bradley N., Shumyatsky, Gleb, Shahim, Pashtun-Poh, DeKosky, Steven T., Stone, James R., Peskind, Elaine, Blennow, Kaj, Zetterberg, Henrik, Chance, Steven A., Torso, Mario, Kostakoglu, Lale, Sano, Mary, Hof, Patrick R., Ahlers, Stephen T., Gandy, Sam, and Elder, Gregory A.
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- 2021
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29. Consensus classification of posterior cortical atrophy
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Crutch, Sebastian J, Schott, Jonathan M, Rabinovici, Gil D, Murray, Melissa, Snowden, Julie S, van der Flier, Wiesje M, Dickerson, Bradford C, Vandenberghe, Rik, Ahmed, Samrah, Bak, Thomas H, Boeve, Bradley F, Butler, Christopher, Cappa, Stefano F, Ceccaldi, Mathieu, de Souza, Leonardo Cruz, Dubois, Bruno, Felician, Olivier, Galasko, Douglas, Graff‐Radford, Jonathan, Graff‐Radford, Neill R, Hof, Patrick R, Krolak‐Salmon, Pierre, Lehmann, Manja, Magnin, Eloi, Mendez, Mario F, Nestor, Peter J, Onyike, Chiadi U, Pelak, Victoria S, Pijnenburg, Yolande, Primativo, Silvia, Rossor, Martin N, Ryan, Natalie S, Scheltens, Philip, Shakespeare, Timothy J, González, Aida Suárez, Tang‐Wai, David F, Yong, Keir XX, Carrillo, Maria, Fox, Nick C, and Area, Alzheimer's Association ISTAART Atypical Alzheimer's Disease and Associated Syndromes Professional Interest
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Biomedical and Clinical Sciences ,Clinical Sciences ,Aging ,Rare Diseases ,Neurosciences ,Neurodegenerative ,Neurological ,Brain ,Brain Diseases ,Humans ,Posterior cortical atrophy ,Alzheimer's disease ,Clinico-radiological syndrome ,Pathophysiology ,Biomarker ,Alzheimer's Association ISTAART Atypical Alzheimer's Disease and Associated Syndromes Professional Interest Area ,Geriatrics ,Clinical sciences ,Biological psychology - Abstract
IntroductionA classification framework for posterior cortical atrophy (PCA) is proposed to improve the uniformity of definition of the syndrome in a variety of research settings.MethodsConsensus statements about PCA were developed through a detailed literature review, the formation of an international multidisciplinary working party which convened on four occasions, and a Web-based quantitative survey regarding symptom frequency and the conceptualization of PCA.ResultsA three-level classification framework for PCA is described comprising both syndrome- and disease-level descriptions. Classification level 1 (PCA) defines the core clinical, cognitive, and neuroimaging features and exclusion criteria of the clinico-radiological syndrome. Classification level 2 (PCA-pure, PCA-plus) establishes whether, in addition to the core PCA syndrome, the core features of any other neurodegenerative syndromes are present. Classification level 3 (PCA attributable to AD [PCA-AD], Lewy body disease [PCA-LBD], corticobasal degeneration [PCA-CBD], prion disease [PCA-prion]) provides a more formal determination of the underlying cause of the PCA syndrome, based on available pathophysiological biomarker evidence. The issue of additional syndrome-level descriptors is discussed in relation to the challenges of defining stages of syndrome severity and characterizing phenotypic heterogeneity within the PCA spectrum.DiscussionThere was strong agreement regarding the definition of the core clinico-radiological syndrome, meaning that the current consensus statement should be regarded as a refinement, development, and extension of previous single-center PCA criteria rather than any wholesale alteration or redescription of the syndrome. The framework and terminology may facilitate the interpretation of research data across studies, be applicable across a broad range of research scenarios (e.g., behavioral interventions, pharmacological trials), and provide a foundation for future collaborative work.
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- 2017
30. Multisite Assessment of Aging-Related Tau Astrogliopathy (ARTAG).
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Kovacs, Gabor G, Xie, Sharon X, Lee, Edward B, Robinson, John L, Caswell, Carrie, Irwin, David J, Toledo, Jon B, Johnson, Victoria E, Smith, Douglas H, Alafuzoff, Irina, Attems, Johannes, Bencze, Janos, Bieniek, Kevin F, Bigio, Eileen H, Bodi, Istvan, Budka, Herbert, Dickson, Dennis W, Dugger, Brittany N, Duyckaerts, Charles, Ferrer, Isidro, Forrest, Shelley L, Gelpi, Ellen, Gentleman, Stephen M, Giaccone, Giorgio, Grinberg, Lea T, Halliday, Glenda M, Hatanpaa, Kimmo J, Hof, Patrick R, Hofer, Monika, Hortobágyi, Tibor, Ironside, James W, King, Andrew, Kofler, Julia, Kövari, Enikö, Kril, Jillian J, Love, Seth, Mackenzie, Ian R, Mao, Qinwen, Matej, Radoslav, McLean, Catriona, Munoz, David G, Murray, Melissa E, Neltner, Janna, Nelson, Peter T, Ritchie, Diane, Rodriguez, Roberta D, Rohan, Zdenek, Rozemuller, Annemieke, Sakai, Kenji, Schultz, Christian, Seilhean, Danielle, Smith, Vanessa, Tacik, Pawel, Takahashi, Hitoshi, Takao, Masaki, Rudolf Thal, Dietmar, Weis, Serge, Wharton, Stephen B, White, Charles L, Woulfe, John M, Yamada, Masahito, and Trojanowski, John Q
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Dementia ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Frontotemporal Dementia (FTD) ,Aging ,Neurodegenerative ,Acquired Cognitive Impairment ,Brain Disorders ,Alzheimer's Disease ,Aged ,Aged ,80 and over ,Astrocytes ,Female ,Humans ,Male ,Middle Aged ,Severity of Illness Index ,Tauopathies ,tau Proteins ,ARTAG ,Digital pathology ,Interrater agreement ,Neuropathology ,Tau ,Tau-astrogliopathy ,Clinical Sciences ,Neurosciences ,Neurology & Neurosurgery - Abstract
Aging-related tau astrogliopathy (ARTAG) is a recently introduced terminology. To facilitate the consistent identification of ARTAG and to distinguish it from astroglial tau pathologies observed in the primary frontotemporal lobar degeneration tauopathies we evaluated how consistently neuropathologists recognize (1) different astroglial tau immunoreactivities, including those of ARTAG and those associated with primary tauopathies (Study 1); (2) ARTAG types (Study 2A); and (3) ARTAG severity (Study 2B). Microphotographs and scanned sections immunostained for phosphorylated tau (AT8) were made available for download and preview. Percentage of agreement and kappa values with 95% confidence interval (CI) were calculated for each evaluation. The overall agreement for Study 1 was >60% with a kappa value of 0.55 (95% CI 0.433-0.645). Moderate agreement (>90%, kappa 0.48, 95% CI 0.457-0.900) was reached in Study 2A for the identification of ARTAG pathology for each ARTAG subtype (kappa 0.37-0.72), whereas fair agreement (kappa 0.40, 95% CI 0.341-0.445) was reached for the evaluation of ARTAG severity. The overall assessment of ARTAG showed moderate agreement (kappa 0.60, 95% CI 0.534-0.653) among raters. Our study supports the application of the current harmonized evaluation strategy for ARTAG with a slight modification of the evaluation of its severity.
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- 2017
31. The nucleus accumbens and ventral pallidum exhibit greater dopaminergic innervation in humans compared to other primates
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Hirter, Kristen N., Miller, Elaine N., Stimpson, Cheryl D., Phillips, Kimberley A., Hopkins, William D., Hof, Patrick R., Sherwood, Chet C., Lovejoy, C. Owen, and Raghanti, Mary Ann
- Published
- 2021
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32. PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival
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Yoon, YoneJung, Voloudakis, Georgios, Doran, Nathan, Zhang, Emily, Dimovasili, Christina, Chen, Lei, Shao, Zhiping, Darmanis, Spyros, Tang, Cheuk, Tang, Jun, Wang, Victoria X., Hof, Patrick R., Robakis, Nikolaos K., and Georgakopoulos, Anastasios
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- 2021
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33. Autism BrainNet: A Collaboration Between Medical Examiners, Pathologists, Researchers, and Families to Advance the Understanding and Treatment of Autism Spectrum Disorder
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Anderson, Matthew P., Quinton, Reade, Kelly, Karen, Falzon, Andrew, Halladay, Alycia, Schumann, Cynthia M., Hof, Patrick R., Tamminga, Carol A., Hare, Carolyn Komich, and Amaral, David G.
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Brain research -- Management ,Pervasive developmental disorders -- Research ,Medical cooperation -- Aims and objectives ,Forensic pathologists -- Aims and objectives -- Alliances and partnerships ,Medical examiners (Law) -- Aims and objectives -- Alliances and partnerships ,Company business management ,Health - Abstract
Context.--Autism spectrum disorder is a neurodevelopmental condition that affects over 1% of the population worldwide. Developing effective preventions and treatments for autism will depend on understanding the neuropathology of the disorder. While evidence from magnetic resonance imaging indicates altered development of the autistic brain, it lacks the resolution needed to identify the cellular and molecular underpinnings of the disorder. Postmortem studies of human brain tissue currently represent the only viable option to pursuing these critical studies. Historically, the availability of autism brain tissue has been extremely limited. Objective.--To overcome this limitation, Autism BrainNet, funded by the Simons Foundation, was formed as a network of brain collection sites that work in a coordinated fashion to develop a library of human postmortem brain tissues for distribution to researchers worldwide. Autism BrainNet has collection sites (or Nodes) in California, Texas, and Massachusetts; affiliated, international Nodes are located in Oxford, England and Montreal, Quebec, Canada. Data Sources.--Pubmed, Autism BrainNet. Conclusions.--Because the death of autistic individuals is often because of an accident, drowning, suicide, or sudden unexpected death in epilepsy, they often are seen in a medical examiner's or coroner's office. Yet, autism is rarely considered when evaluating the cause of death. Advances in our understanding of chronic traumatic encephalopathy have occurred because medical examiners and neuropathologists questioned whether a pathologic change might exist in individuals who played contact sports and later developed severe behavioral problems. This article highlights the potential for equally significant breakthroughs in autism arising from the proactive efforts of medical examiners, pathologists, and coroners in partnership with Autism BrainNet. doi: 10.5858/arpa.2020-0164-RA, Autism Spectrum Disorder (ASD) affects 1 of 59 people in the United States, and likely worldwide, with 4 times as many males diagnosed compared with females. (1) According to the [...]
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- 2021
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34. Where Do Core Thalamocortical Axons Terminate in Mammalian Neocortex When There Is No Cytoarchitecturally Distinct Layer 4?
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Bhagwandin, Adhil, Molnár, Zoltán, Bertelsen, Mads F., Karlsson, Karl Æ., Alagaili, Abdulaziz N., Bennett, Nigel C., Hof, Patrick R., Kaswera‐Kyamakya, Consolate, Gilissen, Emmanuel, Jayakumar, Jaikishan, and Manger, Paul R.
- Abstract
Although the mammalian cerebral cortex is most often described as a hexalaminar structure, there are cortical areas (primary motor cortex) and species (elephants, cetaceans, and hippopotami), where a cytoarchitecturally indistinct, or absent, layer 4 is noted. Thalamocortical projections from the core, or first order, thalamic system terminate primarily in layers 4/inner 3. We explored the termination sites of core thalamocortical projections in cortical areas and in species where there is no cytoarchitecturally distinct layer 4 using the immunolocalization of vesicular glutamate transporter 2, a known marker of core thalamocortical axon terminals, in 31 mammal species spanning the eutherian radiation. Several variations from the canonical cortical column outline of layer 4 and core thalamocortical inputs were noted. In shrews/microchiropterans, layer 4 was present, but many core thalamocortical projections terminated in layer 1 in addition to layers 4 and inner 3. In primate primary visual cortex, the sublaminated layer 4 was associated with a specialized core thalamocortical projection pattern. In primate primary motor cortex, no cytoarchitecturally distinct layer 4 was evident and the core thalamocortical projections terminated throughout layer 3. In the African elephant, cetaceans, and river hippopotamus, no cytoarchitecturally distinct layer 4 was observed and core thalamocortical projections terminated primarily in inner layer 3 and less densely in outer layer 3. These findings are contextualized in terms of cortical processing, perception, and the evolutionary trajectory leading to an indistinct or absent cortical layer 4. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
35. Effects of heterologous human tau protein expression in yeast models of proteotoxic stress response.
- Author
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Zubčić, Klara, Franić, Dina, Pravica, Mihaela, Hof, Patrick R., Šimić, Goran, and Boban, Mirta
- Subjects
TAU proteins ,PROTEIN expression ,YEAST ,ALZHEIMER'S disease ,NEUROFIBRILLARY tangles - Abstract
Background: The primary histological characteristic of Alzheimer's disease is the presence of neurofibrillary tangles, which are large aggregates of tau protein. Aging is the primary risk factor for the development of Alzheimer's disease, however, the underlying causes of tau protein aggregation and toxicity are unclear. Aims: Here we investigated tau aggregation and toxicity under the conditions of compromised protein homeostasis. Methods: We used heterologous expression of human tau protein in the unicellular eukaryote yeast Saccharomyces cerevisiae with evolutionarily conserved protein quality control pathways and examined tau‐dependent toxicity and aggregation using growth assays, fluorescence microscopy, and a split luciferase‐based reporter NanoBiT. Results: Tau protein expressed in yeast under mild proteotoxic stress, or in mutants with impaired pathways for proteotoxic stress response, did not lead to synthetic toxicity or the formation of obvious aggregates. Chronologically old cells also did not develop observable tau aggregates. Our examination of tau oligomerization in living cells using NanoBiT reporter suggests that tau does not form significant levels of oligomers under basal conditions or under mild proteotoxic stress. Conclusion: Together our data suggest that human tau protein does not represent a major burden to the protein quality control system in yeast cells. When expressed in yeast Saccharomyces cerevisiae cell model under the conditions of compromised protein homeostasis, human tau protein does not form visible aggregates or result in toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
36. Comparative anatomy of the caudate nucleus in canids and felids: Associations with brain size, curvature, cross‐sectional properties, and behavioral ecology.
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Foster, Michael, Dwibhashyam, Sai, Patel, Devan, Gupta, Kanika, Matz, Olivia C., Billings, Brendon K., Bitterman, Kathleen, Bertelson, Mads, Tang, Cheuk Y., Mars, Rogier B., Raghanti, Mary Ann, Hof, Patrick R., Sherwood, Chet C., Manger, Paul R., and Spocter, Muhammad A.
- Abstract
The evolutionary history of canids and felids is marked by a deep time separation that has uniquely shaped their behavior and phenotype toward refined predatory abilities. The caudate nucleus is a subcortical brain structure associated with both motor control and cognitive, emotional, and executive functions. We used a combination of three‐dimensional imaging, allometric scaling, and structural analyses to compare the size and shape characteristics of the caudate nucleus. The sample consisted of MRI scan data obtained from six canid species (Canis lupus lupus, Canis latrans, Chrysocyon brachyurus, Lycaon pictus, Vulpes vulpes, Vulpes zerda), two canid subspecies (Canis lupus familiaris, Canis lupus dingo), as well as three felids (Panthera tigris, Panthera uncia, Felis silvestris catus). Results revealed marked conservation in the scaling and shape attributes of the caudate nucleus across species, with only slight deviations. We hypothesize that observed differences in caudate nucleus size and structure for the domestic canids are reflective of enhanced cognitive and emotional pathways that possibly emerged during domestication. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
37. The Resource Identification Initiative: A Cultural Shift in Publishing
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Bandrowski, Anita, Brush, Matthew, Grethe, Jeffery S, Haendel, Melissa A, Kennedy, David N, Hill, Sean, Hof, Patrick R, Martone, Maryann E, Pols, Maaike, Tan, Serena S, Washington, Nicole, Zudilova-Seinstra, Elena, Vasilevsky, Nicole, and RINL Resource Identification Initiative
- Subjects
Biological Sciences ,Bioinformatics and Computational Biology ,2.6 Resources and infrastructure (aetiology) ,Generic health relevance ,Culture ,Datasets as Topic ,Health Resources ,Humans ,Neurosciences ,Pilot Projects ,PubMed ,Publishing ,RRID:nif-0000-25673 ,RRID:nlx_153866 ,Informatics ,Reproducibility ,RINL Resource Identification Initiative ,Biochemistry and Cell Biology ,Neurology & Neurosurgery ,Bioinformatics and computational biology ,Biological psychology - Abstract
A central tenet in support of research reproducibility is the ability to uniquely identify research resources, i.e., reagents, tools, and materials that are used to perform experiments. However, current reporting practices for research resources are insufficient to identify the exact resources that are reported or to answer basic questions such as "How did other studies use resource X?" To address this issue, the Resource Identification Initiative was launched as a pilot project to improve the reporting standards for research resources in the methods sections of papers and thereby improve identifiability and scientific reproducibility. The pilot engaged over 25 biomedical journal editors from most major publishers, as well as scientists and funding officials. Authors were asked to include Research Resource Identifiers (RRIDs) in their manuscripts prior to publication for three resource types: antibodies, model organisms, and tools (i.e., software and databases). RRIDs are assigned by an authoritative database, for example a model organism database, for each type of resource. To make it easier for authors to obtain RRIDs, resources were aggregated from the appropriate databases and their RRIDs made available in a central web portal ( http://scicrunch.org/resources ). RRIDs meet three key criteria: they are machine readable, free to generate and access, and are consistent across publishers and journals. The pilot was launched in February of 2014 and over 300 papers have appeared that report RRIDs. The number of journals participating has expanded from the original 25 to more than 40 with RRIDs appearing in 62 different journals to date. Here, we present an overview of the pilot project and its outcomes to date. We show that authors are able to identify resources and are supportive of the goals of the project. Identifiability of the resources post-pilot showed a dramatic improvement for all three resource types, suggesting that the project has had a significant impact on identifiability of research resources.
- Published
- 2016
38. Aging-related tau astrogliopathy (ARTAG): harmonized evaluation strategy
- Author
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Kovacs, Gabor G, Ferrer, Isidro, Grinberg, Lea T, Alafuzoff, Irina, Attems, Johannes, Budka, Herbert, Cairns, Nigel J, Crary, John F, Duyckaerts, Charles, Ghetti, Bernardino, Halliday, Glenda M, Ironside, James W, Love, Seth, Mackenzie, Ian R, Munoz, David G, Murray, Melissa E, Nelson, Peter T, Takahashi, Hitoshi, Trojanowski, John Q, Ansorge, Olaf, Arzberger, Thomas, Baborie, Atik, Beach, Thomas G, Bieniek, Kevin F, Bigio, Eileen H, Bodi, Istvan, Dugger, Brittany N, Feany, Mel, Gelpi, Ellen, Gentleman, Stephen M, Giaccone, Giorgio, Hatanpaa, Kimmo J, Heale, Richard, Hof, Patrick R, Hofer, Monika, Hortobágyi, Tibor, Jellinger, Kurt, Jicha, Gregory A, Ince, Paul, Kofler, Julia, Kövari, Enikö, Kril, Jillian J, Mann, David M, Matej, Radoslav, McKee, Ann C, McLean, Catriona, Milenkovic, Ivan, Montine, Thomas J, Murayama, Shigeo, Lee, Edward B, Rahimi, Jasmin, Rodriguez, Roberta D, Rozemüller, Annemieke, Schneider, Julie A, Schultz, Christian, Seeley, William, Seilhean, Danielle, Smith, Colin, Tagliavini, Fabrizio, Takao, Masaki, Thal, Dietmar Rudolf, Toledo, Jon B, Tolnay, Markus, Troncoso, Juan C, Vinters, Harry V, Weis, Serge, Wharton, Stephen B, White, Charles L, Wisniewski, Thomas, Woulfe, John M, Yamada, Masahito, and Dickson, Dennis W
- Subjects
Biomedical and Clinical Sciences ,Neurosciences ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Rare Diseases ,Alzheimer's Disease ,Aging ,Frontotemporal Dementia (FTD) ,Brain Disorders ,Dementia ,Neurodegenerative ,Acquired Cognitive Impairment ,Neurological ,Animals ,Astrocytes ,Brain ,Humans ,Neuroglia ,Tauopathies ,tau Proteins ,ARTAG ,Tau astrogliopathy ,Tau ,Clinical Sciences ,Neurology & Neurosurgery - Abstract
Pathological accumulation of abnormally phosphorylated tau protein in astrocytes is a frequent, but poorly characterized feature of the aging brain. Its etiology is uncertain, but its presence is sufficiently ubiquitous to merit further characterization and classification, which may stimulate clinicopathological studies and research into its pathobiology. This paper aims to harmonize evaluation and nomenclature of aging-related tau astrogliopathy (ARTAG), a term that refers to a morphological spectrum of astroglial pathology detected by tau immunohistochemistry, especially with phosphorylation-dependent and 4R isoform-specific antibodies. ARTAG occurs mainly, but not exclusively, in individuals over 60 years of age. Tau-immunoreactive astrocytes in ARTAG include thorn-shaped astrocytes at the glia limitans and in white matter, as well as solitary or clustered astrocytes with perinuclear cytoplasmic tau immunoreactivity that extends into the astroglial processes as fine fibrillar or granular immunopositivity, typically in gray matter. Various forms of ARTAG may coexist in the same brain and might reflect different pathogenic processes. Based on morphology and anatomical distribution, ARTAG can be distinguished from primary tauopathies, but may be concurrent with primary tauopathies or other disorders. We recommend four steps for evaluation of ARTAG: (1) identification of five types based on the location of either morphologies of tau astrogliopathy: subpial, subependymal, perivascular, white matter, gray matter; (2) documentation of the regional involvement: medial temporal lobe, lobar (frontal, parietal, occipital, lateral temporal), subcortical, brainstem; (3) documentation of the severity of tau astrogliopathy; and (4) description of subregional involvement. Some types of ARTAG may underlie neurological symptoms; however, the clinical significance of ARTAG is currently uncertain and awaits further studies. The goal of this proposal is to raise awareness of astroglial tau pathology in the aged brain, facilitating communication among neuropathologists and researchers, and informing interpretation of clinical biomarkers and imaging studies that focus on tau-related indicators.
- Published
- 2016
39. Quantification of neurons in the hippocampal formation of chimpanzees: comparison to rhesus monkeys and humans
- Author
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Rogers Flattery, Christina N., Rosen, Rebecca F., Farberg, Aaron S., Dooyema, Jeromy M., Hof, Patrick R., Sherwood, Chet C., Walker, Lary C., and Preuss, Todd M.
- Published
- 2020
- Full Text
- View/download PDF
40. Transcriptome-wide isoform-level dysregulation in ASD, schizophrenia, and bipolar disorder
- Author
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PsychENCODE Consortium, Gandal, Michael J., Zhang, Pan, Hadjimichael, Evi, Walker, Rebecca L., Chen, Chao, Liu, Shuang, Won, Hyejung, van Bakel, Harm, Varghese, Merina, Wang, Yongjun, Shieh, Annie W., Haney, Jillian, Parhami, Sepideh, Belmont, Judson, Kim, Minsoo, Losada, Patricia Moran, Khan, Zenab, Mleczko, Justyna, Xia, Yan, Dai, Rujia, Wang, Daifeng, Yang, Yucheng T., Xu, Min, Fish, Kenneth, Hof, Patrick R., Warrell, Jonathan, Fitzgerald, Dominic, White, Kevin, Jaffe, Andrew E., Peters, Mette A., Gerstein, Mark, Liu, Chunyu, Iakoucheva, Lilia M., Pinto, Dalila, and Geschwind, Daniel H.
- Published
- 2018
41. Low-level blast exposure induces chronic vascular remodeling, perivascular astrocytic degeneration and vascular-associated neuroinflammation
- Author
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Gama Sosa, Miguel A., De Gasperi, Rita, Pryor, Dylan, Perez Garcia, Georgina S., Perez, Gissel M., Abutarboush, Rania, Kawoos, Usmah, Hogg, Seth, Ache, Benjamin, Janssen, William G., Sowa, Allison, Tetreault, Timothy, Cook, David G., Tappan, Susan J., Gandy, Sam, Hof, Patrick R., Ahlers, Stephen T., and Elder, Gregory A.
- Published
- 2021
- Full Text
- View/download PDF
42. Laterality and region-specific tau phosphorylation correlate with PTSD-related behavioral traits in rats exposed to repetitive low-level blast
- Author
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Perez Garcia, Georgina, De Gasperi, Rita, Gama Sosa, Miguel A., Perez, Gissel M., Otero-Pagan, Alena, Pryor, Dylan, Abutarboush, Rania, Kawoos, Usmah, Hof, Patrick R., Dickstein, Dara L., Cook, David G., Gandy, Sam, Ahlers, Stephen T., and Elder, Gregory A.
- Published
- 2021
- Full Text
- View/download PDF
43. Amplification of potential thermogenetic mechanisms in cetacean brains compared to artiodactyl brains
- Author
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Manger, Paul R., Patzke, Nina, Spocter, Muhammad A., Bhagwandin, Adhil, Karlsson, Karl Æ., Bertelsen, Mads F., Alagaili, Abdulaziz N., Bennett, Nigel C., Mohammed, Osama B., Herculano-Houzel, Suzana, Hof, Patrick R., and Fuxe, Kjell
- Published
- 2021
- Full Text
- View/download PDF
44. Improvement of magnetic resonance imaging using a wireless radiofrequency resonator array
- Author
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Alipour, Akbar, Seifert, Alan C., Delman, Bradley N., Robson, Philip M., Shrivastava, Raj, Hof, Patrick R., Adriany, Gregor, Fayad, Zahi A., and Balchandani, Priti
- Published
- 2021
- Full Text
- View/download PDF
45. Neural architecture of the vertebrate brain: implications for the interaction between emotion and cognition
- Author
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Pessoa, Luiz, Medina, Loreta, Hof, Patrick R., and Desfilis, Ester
- Published
- 2019
- Full Text
- View/download PDF
46. The Resource Identification Initiative: A cultural shift in publishing
- Author
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Bandrowski, Anita, Brush, Matthew, Grethe, Jeffery S, Haendel, Melissa A, Kennedy, David N, Hill, Sean, Hof, Patrick R, Martone, Maryann E, Pols, Maaike, Tan, Serena, Washington, Nicole, Zudilova-Seinstra, Elena, and Vasilevsky, Nicole
- Subjects
Information and Computing Sciences ,Biological Sciences ,Bioinformatics and Computational Biology ,Library and Information Studies ,2.6 Resources and infrastructure (aetiology) ,Generic health relevance ,Resource Identification Initiative Members are listed here: https://www.force11.org/node/4463/members ,Multi-centre initiative ,Post-pilot data ,Pre-pilot data ,Publishing ,Resource identifiers ,Biochemistry and Cell Biology ,Clinical Sciences ,Oncology and Carcinogenesis - Abstract
A central tenet in support of research reproducibility is the ability to uniquely identify research resources, i.e., reagents, tools, and materials that are used to perform experiments. However, current reporting practices for research resources are insufficient to allow humans and algorithms to identify the exact resources that are reported or answer basic questions such as "What other studies used resource X?" To address this issue, the Resource Identification Initiative was launched as a pilot project to improve the reporting standards for research resources in the methods sections of papers and thereby improve identifiability and reproducibility. The pilot engaged over 25 biomedical journal editors from most major publishers, as well as scientists and funding officials. Authors were asked to include Research Resource Identifiers (RRIDs) in their manuscripts prior to publication for three resource types: antibodies, model organisms, and tools (including software and databases). RRIDs represent accession numbers assigned by an authoritative database, e.g., the model organism databases, for each type of resource. To make it easier for authors to obtain RRIDs, resources were aggregated from the appropriate databases and their RRIDs made available in a central web portal ( www.scicrunch.org/resources). RRIDs meet three key criteria: they are machine readable, free to generate and access, and are consistent across publishers and journals. The pilot was launched in February of 2014 and over 300 papers have appeared that report RRIDs. The number of journals participating has expanded from the original 25 to more than 40. Here, we present an overview of the pilot project and its outcomes to date. We show that authors are generally accurate in performing the task of identifying resources and supportive of the goals of the project. We also show that identifiability of the resources pre- and post-pilot showed a dramatic improvement for all three resource types, suggesting that the project has had a significant impact on reproducibility relating to research resources.
- Published
- 2015
47. Primary age-related tauopathy (PART): a common pathology associated with human aging
- Author
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Crary, John F, Trojanowski, John Q, Schneider, Julie A, Abisambra, Jose F, Abner, Erin L, Alafuzoff, Irina, Arnold, Steven E, Attems, Johannes, Beach, Thomas G, Bigio, Eileen H, Cairns, Nigel J, Dickson, Dennis W, Gearing, Marla, Grinberg, Lea T, Hof, Patrick R, Hyman, Bradley T, Jellinger, Kurt, Jicha, Gregory A, Kovacs, Gabor G, Knopman, David S, Kofler, Julia, Kukull, Walter A, Mackenzie, Ian R, Masliah, Eliezer, McKee, Ann, Montine, Thomas J, Murray, Melissa E, Neltner, Janna H, Santa-Maria, Ismael, Seeley, William W, Serrano-Pozo, Alberto, Shelanski, Michael L, Stein, Thor, Takao, Masaki, Thal, Dietmar R, Toledo, Jonathan B, Troncoso, Juan C, Vonsattel, Jean Paul, White, Charles L, Wisniewski, Thomas, Woltjer, Randall L, Yamada, Masahito, and Nelson, Peter T
- Subjects
Biomedical and Clinical Sciences ,Neurosciences ,Neurodegenerative ,Brain Disorders ,Aging ,Alzheimer's Disease ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Dementia ,Alzheimer's Disease Related Dementias (ADRD) ,Acquired Cognitive Impairment ,Aetiology ,Detection ,screening and diagnosis ,4.2 Evaluation of markers and technologies ,2.1 Biological and endogenous factors ,4.1 Discovery and preclinical testing of markers and technologies ,Neurological ,Brain ,Diagnosis ,Differential ,Humans ,Plaque ,Amyloid ,Tauopathies ,Terminology as Topic ,TPSD ,TOD ,Braak ,Neuropathology ,Consensus ,Clinical Sciences ,Neurology & Neurosurgery - Abstract
We recommend a new term, "primary age-related tauopathy" (PART), to describe a pathology that is commonly observed in the brains of aged individuals. Many autopsy studies have reported brains with neurofibrillary tangles (NFTs) that are indistinguishable from those of Alzheimer's disease (AD), in the absence of amyloid (Aβ) plaques. For these "NFT+/Aβ-" brains, for which formal criteria for AD neuropathologic changes are not met, the NFTs are mostly restricted to structures in the medial temporal lobe, basal forebrain, brainstem, and olfactory areas (bulb and cortex). Symptoms in persons with PART usually range from normal to amnestic cognitive changes, with only a minority exhibiting profound impairment. Because cognitive impairment is often mild, existing clinicopathologic designations, such as "tangle-only dementia" and "tangle-predominant senile dementia", are imprecise and not appropriate for most subjects. PART is almost universally detectable at autopsy among elderly individuals, yet this pathological process cannot be specifically identified pre-mortem at the present time. Improved biomarkers and tau imaging may enable diagnosis of PART in clinical settings in the future. Indeed, recent studies have identified a common biomarker profile consisting of temporal lobe atrophy and tauopathy without evidence of Aβ accumulation. For both researchers and clinicians, a revised nomenclature will raise awareness of this extremely common pathologic change while providing a conceptual foundation for future studies. Prior reports that have elucidated features of the pathologic entity we refer to as PART are discussed, and working neuropathological diagnostic criteria are proposed.
- Published
- 2014
48. Anterior insular cortex is a bottleneck of cognitive control
- Author
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Wu, Tingting, Wang, Xingchao, Wu, Qiong, Spagna, Alfredo, Yang, Jiaqi, Yuan, Changhe, Wu, Yanhong, Gao, Zhixian, Hof, Patrick R., and Fan, Jin
- Published
- 2019
- Full Text
- View/download PDF
49. Subfield-specific tractography of the hippocampus in epilepsy patients at 7 Tesla
- Author
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Rutland, John W., Feldman, Rebecca E., Delman, Bradley N., Panov, Fedor, Fields, Madeline C., Marcuse, Lara V., Hof, Patrick R., Lin, Hung-Mo, and Balchandani, Priti
- Published
- 2018
- Full Text
- View/download PDF
50. NSF Workshop Report: Discovering General Principles of Nervous System Organization by Comparing Brain Maps across Species
- Author
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Striedter, Georg F, Belgard, T Grant, Chen, Chun-Chun, Davis, Fred P, Finlay, Barbara L, Güntürkün, Onur, Hale, Melina E, Harris, Julie A, Hecht, Erin E, Hof, Patrick R, Hofmann, Hans A, Holland, Linda Z, Iwaniuk, Andrew N, Jarvis, Erich D, Karten, Harvey J, Katz, Paul S, Kristan, William B, Macagno, Eduardo R, Mitra, Partha P, Moroz, Leonid L, Preuss, Todd M, Ragsdale, Clifton W, Sherwood, Chet C, Stevens, Charles F, Stüttgen, Maik C, Tsumoto, Tadaharu, and Wilczynski, Walter
- Subjects
Biological Sciences ,Genetics ,Neurosciences ,Anatomy ,Comparative ,Animals ,Biological Evolution ,Brain ,Brain Mapping ,Humans ,Species Specificity ,Medical and Health Sciences ,Neurology & Neurosurgery ,Biological sciences ,Biomedical and clinical sciences ,Psychology - Abstract
Efforts to understand nervous system structure and function have received new impetus from the federal Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative. Comparative analyses can contribute to this effort by leading to the discovery of general principles of neural circuit design, information processing, and gene-structure-function relationships that are not apparent from studies on single species. We here propose to extend the comparative approach to nervous system 'maps' comprising molecular, anatomical, and physiological data. This research will identify which neural features are likely to generalize across species, and which are unlikely to be broadly conserved. It will also suggest causal relationships between genes, development, adult anatomy, physiology, and, ultimately, behavior. These causal hypotheses can then be tested experimentally. Finally, insights from comparative research can inspire and guide technological development. To promote this research agenda, we recommend that teams of investigators coalesce around specific research questions and select a set of 'reference species' to anchor their comparative analyses. These reference species should be chosen not just for practical advantages, but also with regard for their phylogenetic position, behavioral repertoire, well-annotated genome, or other strategic reasons. We envision that the nervous systems of these reference species will be mapped in more detail than those of other species. The collected data may range from the molecular to the behavioral, depending on the research question. To integrate across levels of analysis and across species, standards for data collection, annotation, archiving, and distribution must be developed and respected. To that end, it will help to form networks or consortia of researchers and centers for science, technology, and education that focus on organized data collection, distribution, and training. These activities could be supported, at least in part, through existing mechanisms at NSF, NIH, and other agencies. It will also be important to develop new integrated software and database systems for cross-species data analyses. Multidisciplinary efforts to develop such analytical tools should be supported financially. Finally, training opportunities should be created to stimulate multidisciplinary, integrative research into brain structure, function, and evolution.
- Published
- 2014
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