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Objective To investigate the effect of care bundles based on the concept of enhanced recovery after surgery in perioperative nursing of patients undergoing laparoscopic cholecystectomy. Methods A total of 260 cases of laparoscopic cholecystectomy were randomly divided into observation group (n=130, received routine nursing care) and control group (n=130, received care bundles based on the concept of enhanced recovery). Postoperative recovery condition, incidence of complications and postoperative pain were compared between the two groups. Results At 2, 6 and 24 h after surgery, the Visual Analogue Scale of pain in the observation group were lower than those in the control group, with a significant difference(P＜0. 05). The time to first postoperative exhaustion, time to off-bed activities and hospital stays in the observation group were shorter than those in the control group (P＜0. 05). The incidence of vomiting, incision infection, and bile leakage in the control group was significantly higher than that in the observation group, with a significant difference (P＜0. 05). Conclusion The application of care bundles based on the concept of enhanced recovery after surgery are conducive to the postoperative recovery of patients undergoing laparoscopic cholecystectomy, as well as the improvement of postoperative quality of life. (目的 探讨基于加速康复外科理念的集束化干预措施在腹腔镜胆囊切除患者围术期护理中的应用效果。方法 选取2021年1月—2023年1月某三甲肝胆专科医院收治的260例行腹腔镜胆囊切除术的患者为研究对象, 随机分为两组: 对照组(n=130)围手术期间给予常规护理; 观察组(n=130)实施基于加速康复外科理念的集束化干预措施。比较两组术后恢复情况、并发症发生率以及术后疼痛情况。结果 术后2 h、6 h、24 h观察组患者疼痛视觉模拟(VAS)评分低于对照组, 差异有统计学意义(P＜0. 05)。与对照组相比, 观察组患者术后排气时间、下床时间和住院天数均缩短, 差异有统计学意义(P＜0. 05)。对照组呕吐、切口感染与胆漏发生率高于观察组, 差异有统计学意义(P＜0. 05)。结论 基于加速康复外科理念的集束化干预措更有利于患者术后康复, 提高术后生活质量 。)

Objective To investigate the application effect of establishment of chief nurse position in training of newly recruited nurses. Methods The chief nurse position was established for the duty management of newly recruited nurses. Totally 45 newly recruited nurses who participated in the clinical practice after establishment of chief nurse position were included as the study, another 45 newly recruited nurses who received routine clinical teaching and practice were included as the control group. The nurses’ job satisfaction, anxiety states and turnover intention were measured and compared between two groups. Results In job satisfaction assessment, scores of Work-Family Balance, Relationship with colleagues, control and responsibility; Being Praised and Recognized and Responsibility Assignment items in the study group were higher than those in the control group respectively(P＜0. 05). The Self-rating Anxiety Scale in the study group was lower than that in the control group(P＜0. 05). The rate of turnover intention in the study group was lower than that in the control group(P＜0. 05). Conclusion The establishment of chief nurse position, by increasing job security, is effective to improve the nurses’ confidence and quality of nursing practice, reduce the fear of working independently and turnover intention, enhance the nursing safety in nursing practice. (目的 探讨科室总护士岗位设立在新入职护士值班管理中的应用效果。方法 科室通过设立总护士岗位加强新入职护士值班管理, 采用目的抽样法将本科室2020年7月—2022年7月设立总护士岗位以来培养的45名新入职护士作为研究组, 选取医院其他科室2020年7月—2022年7月以传统带教方式培养的新入职护士45名作为对照组。采用护士工作满意度量表、焦虑自评量表(SAS)、离职意愿量表进行对比分析。结果 度评价条目8个条目中, 工作与家庭平衡、与同事关系、控制与责任、被称赞与被认可和职责分配条目研究组得分高于对照组, 差异有统计学意义(P＜0. 05)。研究组SAS量表评分低于对照组(P＜0. 01)。研究组离职意愿比例低于对照组(P＜0. 05)。结论 设立科室总护士岗位能够增加新入职护士安全感, 提高了护士工作的自信心和护理工作质量, 降低新入职护士对独立值班的恐惧, 降低新入职护士的离职率, 提高独立值班中的护理安全。)

Background Functional dyspepsia (FD) after ischemic stroke belongs to the category of post-stroke gastrointestinal dysfunction, which is prevalent in middle-aged and elderly patients. Stroke patients may differ from the general population in the influencing factors of FD due to their own somatic and psychological changes. Objective To investigate the associated factors of the severity of functional dyspepsia after ischemic stroke. Methods A total of 180 middle and old aged patients with functional dyspepsia after ischemic stroke were selected from March 2017 to July 2018 in the Department of Acupuncture of the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine. A self-designed dyspepsia symptom score scale was used to evaluate the severity of FD during hospitalization. The patients were divided into mild FD group (score≤7, n=115) and moderate-severe FD group (score>7, n=65) according to the dyspepsia symptom score. Patient data were collected through the medical record system, and multivariate Logistic regression were used to explore the influencing factors of functional dyspepsia severity after ischemic stroke. Results The FD symptom scores of 180 patients was in the range of 3-12 points, with an average of (7.2±1.7) points. Among them, 115 patients (63.9%) had symptom score≤7 points, and 65 patients (36.1%) had symptom score>7 points. The severity of functional dyspepsia patients with different ages and stroke duration (acute, convalescent, sequelae) was statistically different (P

This article discusses the detection of the cfr gene in Citrobacter braakii and the characterization of two cfr-carrying plasmids from a chicken farm in Jiangxi, China. The cfr gene is a multidrug resistance gene that has spread globally in Gram-positive and Gram-negative bacteria. The study identified three cfr-positive strains, including one C. braakii and two E. coli strains, and found that the cfr gene was located on different plasmids in these strains. The article highlights the widespread distribution and rapid dissemination of cfr in animal-derived bacteria and emphasizes the need for monitoring and control of cfr plasmids. [Extracted from the article]

Chickpeas (Cicer arietinum L.) are an important legume crop known for their rich nutrient content, including proteins, carbohydrates, and minerals. Thus, they are enjoyed by people worldwide. In recent years, the production scale of chickpeas has been growing gradually. The planting area of chickpeas represents roughly 35–36% of the total planting area, and the output of the beans is roughly 47–48%. However, the growth and development process of chickpeas is limited by a number of factors, including high temperature, drought, salt stress, and so forth. In particular, high temperatures can reduce the germination rate, photosynthesis, seed setting rate, and filling rate of chickpeas, restricting seed germination, plant growth, and reproductive growth. These changes lead to a decrease in the yield and quality of the crop. Heat shock proteins (HSPs) are small proteins that play an important role in plant defense against abiotic stress. Therefore, in the present study, HSP20 gene family members were identified based on the whole-genome data of chickpeas, and their chromosomal positions, evolutionary relationships, promoter cis-acting elements, and tissue-specific expression patterns were predicted. Subsequently, qRT-PCR was used to detect and analyze the expression characteristics of HSP20 genes under different temperature stress conditions. Ultimately, we identified twenty-one HSP20 genes distributed on seven chromosomes, and their gene family members were found to be relatively conserved, belonging to ten subfamilies. We also found that CaHSP20 promoter regions have many cis-acting elements related to growth and development, hormones, and stress responses. In addition, under high-temperature stress, the relative expression of CaHSP20-17, CaHSP20-20, CaHSP20-7, CaHSP20-3, and CaHSP20-12 increased hundreds or even thousands of times as the temperature increased from 25 °C to 42 °C. Among them, excluding CaHSP20-5, the other five genes all contain 1-2 ABA cis-regulatory elements. This finding indicates that CaHSP20s are involved in the growth and development of chickpeas under heat stress, and the mechanisms of their responses to high-temperature stress may be related to hormone regulation. The results of the present study lay the foundation for exploring HSP20 gene family resources and the molecular mechanisms of heat resistance in chickpeas. Our results can also provide a theoretical basis for breeding high-temperature-resistant chickpea varieties and provide valuable information for the sustainable development of the global chickpea industry. [ABSTRACT FROM AUTHOR]

Hemp seeds are consumed in "Bama longevity villages" in China and are believed to contribute to the locals' longevity. However, the molecular breeding of hemp seeds is limited due to our lack of understanding of molecular regulation and metabolite accumulation at different maturity stages. Here, we conducted metabolomic and transcriptomic analyses of hemp seeds from the Bama region at four maturity stages (S1 to S4). In total, 1231 metabolites of 11 classes were detected in hemp seeds from S1–S4, including 233 flavonoids, 214 phenolic acids, 159 lipids, 118 amino acids and derivatives, 100 alkaloids, 100 organic acids, 71 nucleotides and derivatives, 43 lignans and coumarins, 14 terpenoids, 13 tannins, and 166 others. The metabolomic analysis of hemp seeds (from the Bama region) revealed a higher number of flavonoid metabolites compared with seven other varieties from other regions in China. Hemp seeds are rich in metabolites like cannflavin, trigonelline, citric acid, vitexin, choline alfoscerate, and choline, which may potentially contribute to the longevity of the Bama people. Through transcriptomic and metabolomic analyses, a gradual decrease in the overall expression pattern of genes and metabolite accumulation was observed during seed maturation. Weighted gene co-expression network analysis revealed that two genes (ncbi_115696993 and ncbi_115706674) are involved in regulating main metabolites, while transcription factor association analysis revealed that three transcription factor genes (MYB, NAC, and GRAS) are also involved in regulating the metabolites. The expression pattern of these five candidate genes was further verified by qPCR. Our study provides valuable insights into the metabolic substances during seed maturation and identifies candidate genes that could be utilized for future genetic engineering to enhance the endogenous biosynthesis of health-promoting metabolites in hemp seeds, potentially leading to improved nutritional and medicinal properties. [ABSTRACT FROM AUTHOR]