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The trapping efficiency and stiffness of optical tweezers using radial polarization are evaluated; the ray-tracing method and a proposed measurement method are used for numerical and experimental analyses, respectively. The maximum axial trapping efficiency with radial polarization is 1.84 times that with linear polarization, while the maximum transverse trapping efficiency decreases by 0.58 times. Further, the axial and transverse trapping efficiencies are found to be 1.19 times larger and 0.83 times smaller, respectively, than the values with linear polarization. From the experiments, the axial and transverse stiffness values are 1.2 times larger and 0.8 times smaller, respectively, with radial polarization. Hence, radial polarization enhances the axial trapping properties while reducing the transverse trapping properties. OCIS codes: 140.7010, 350.4855, 230.1040.

A new surface probing technique using the circular motion of an optically-trapped microsphere is proposed for a nanocoordinate measuring system. The probe sphere is oscillated circularly in the plane perpendicular to the probe axis and the circular orbit of the probe sphere is monitored for the detection of the position and normal vector direction of the surface. The principle of detection is based on changes in the circular orbit of the microsphere. When the probe approaches a work surface, the orbit of the probe sphere becomes elliptical. The minor-axis length and the minor-axis angle of the ellipse are then used as parameters to detect the position and normal vector direction of the surface, respectively. In this study, the circular motion probe is shown to have a resolution of position detection of 39 nm, and the accuracy of measuring a normal vector to the surface is on the order of 3[degrees]. OCIS codes: 120.1880, 350.4855.

When a microsphere is trapped near a surface by single-beam gradient force trapping, the standing wave is generated between the microsphere and the surface, where abrupt motion along the optical axis (jumping) is observed corresponding to displacement of the surface. This jumping distance is on the order of a few hundred nanometers. In the vicinity of the surface, intensity of retro-reflected light is increased so that the averaged position of the jumping is shifted up on the order of several micrometers. Therefore wide-range and high-resolution position measurement technique is required. In this article, we proposed to apply a chromatic confocal sensor to measure the axial position of the microsphere in the standing wave. It was experimentally validated that the position of the microsphere could be measured with a resolution of 10 nm and a measuring range of 3 µm. [ABSTRACT FROM PUBLISHER]

Laser trapping is becoming an important technique for microsystem technologies. To apply it to industrial uses, it should be developed to function in air. However, there is not much research about laser trapping in air. One of the reasons is the difficulty of trapping micro-objects. Therefore, we have proposed a new technique to trap micro-objects in air. In particular, we focused our attention on the substrate where the micro-object is set. By applying a textured surface and employing tungsten carbide as the substrate material, the trapping probability was improved by a remarkable amount. Although a certain degree of improvement was attained, the trapping was still not perfect. To ascertain the forces working on the micro-object, an analytical calculation of the adhesion forces and an electromagnetic simulation of the optical forces were implemented. Based on these calculations, we discuss what the most important factor is for successful laser trapping in air. [ABSTRACT FROM AUTHOR]

Positioning technology is one of the most important technologies for developing microsystems. In particular, displacement sensors are necessary for positioning devices with nanoscale accuracy. In this study, we propose a new displacement sensor that uses an interference scale as a linear scale and a laser-trapped microsphere as a sensing probe. This sensor has a wide measuring range, high resolution, and accessibility for narrow target areas. A glass microsphere was optically trapped by means of the laser trapping technique. Between the target surface and the probe, an interference scale was generated along the optical axis. The scale origin was fixed on the target surface. The distance between the probe and the target surface could be measured in terms of the shift in the interference scale. This study investigated the fundamental performance of the sensor. The resolution and accuracy of the sensor were 10 and ±50 nm, respectively; these values could be improved by using trapping lasers having shorter wavelengths. The measurable range was 250 μm. This sensor can provide useful displacement information from a target area having dimensions smaller than 15 μm. In addition, the displacement sensor can measure the distance even for surfaces inclined at angles less than 15°; thus, a flexible arrangement can be used to carry out measurements. In addition, the direction of displacement can be identified. [ABSTRACT FROM AUTHOR]

Atomic force microscopy (AFM) is an effective platform for in vitro manipulation and analysis of living cells in medical and biological sciences. To introduce additional new features and functionalities into a conventional AFM system, we investigated the photocatalytic nanofabrication and intracellular Raman imaging of living cells by employing functionalized AFM probes. Herein, we investigated the effect of indentation speed on the cell membrane perforation of living HeLa cells based on highly localized photochemical oxidation with a catalytic titanium dioxide (TiO2)-functionalized AFM probe. On the basis of force–distance curves obtained during the indentation process, the probability of cell membrane perforation, penetration force, and cell viability was determined quantitatively. Moreover, we explored the possibility of intracellular tip-enhanced Raman spectroscopy (TERS) imaging of molecular dynamics in living cells via an AFM probe functionalized with silver nanoparticles in a homemade Raman system integrated with an inverted microscope. We successfully demonstrated that the intracellular TERS imaging has the potential to visualize distinctly different features in Raman spectra between the nucleus and the cytoplasm of a single living cell and to analyze the dynamic behavior of biomolecules inside a living cell. [ABSTRACT FROM AUTHOR]

A three-dimensional probe is mounted on a specialized gear measuring machine whose measurement target is limited on the working flanks. The newly developed measuring machine has a coordinate measuring mechanism, aiming to be a highly versatile and relatively inexpensive measuring machine. According to our previous research, in addition to the working tooth surface, shape data of the root, bottom and tip profiles can be acquired with a single measurement operation. However, the target object for which this developed method has been applied was only the external cylindrical gear. In this paper, we applied and expanded the development method to the internal cylindrical gear and the evaluation of the axial displacement in the same cross-sectional scanning of the internal helical gear are performed.

Abstract: In the present study, concentrations of 13 elements (Li, Cr, Mn, Cu, Zn, As, Se, Rb, Sr, Mo, Ag, Cd, Hg) were measured in the tissues of the livers, the kidneys, pectoral muscles, lungs and brains of 13 avian species collected from the Kanto area of Japan. The difference in hepatic heavy metal levels of the grey herons from the two sites was compared. Metal levels in the sediment of the Tama River estuary, situated in the Haneda area, were also measured. These results revealed that heavy metal pollution is present in an aquatic area of Haneda. The accumulation patterns of Cu and Zn in the livers of grey herons appeared to be separated into two groups. Additionally, the present study includes the properties of other metal accumulations and their relationships in avian species. [Copyright &y& Elsevier]