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Background: Neoadjuvant radiotherapy and chemotherapy, followed by surgical resection, are standard treatments for locally advanced rectal cancer (LARC). Emerging evidence has shown the efficacy of anti-programmed cell death protein 1 (anti-PD-1) therapy for patients with mismatch repair-deficient (dMMR) colorectal cancer, particularly in managing metastatic disease. Several ongoing clinical trials evaluating the efficacy of anti-PD-1 therapy in patients with dMMR LARC have reported outstanding responses. Patients and methods: Here, we present the VOLTAGE-2 study (EPOC 2201), a non-randomized, single-arm, phase II trial that aims to investigate the efficacy and safety of nivolumab monotherapy for 1 year in patients with dMMR-resectable LARC. Patients with clinical complete response (cCR) or near-complete response (nCR) will be observed with non-operative management (NOM) using the Memorial Sloan Kettering Regression Schema.The primary endpoint will be investigator-determined 2-year cCR rate for nivolumab monotherapy. We will investigate the surrogacy of circulating tumor DNA assay as a cCR using whole-genome sequencing (WGS)-based molecular residual disease (MRD) assay and will evaluate the biomarkers of the response to anti-PD-1 antibody using whole-exome sequencing (WES) plus whole-transcriptome sequencing (WTS)-based tumor genomics and immune microenvironment evaluations. We plan to carry out spatiotemporal trans-omics analyses using artificial intelligence and deep learning-driven genomics, transcriptomics, radiomics, pathomics, colonoscopic imaging, quality of life, and clinical features.

Abstract We present Magnetospheric Multiscale observations of an electron‐scale reconnecting current sheet in the mixing region along the trailing edge of a Kelvin‐Helmholtz vortex during southward interplanetary magnetic field conditions. Within this region, we observe intense electrostatic wave activity, consistent with lower‐hybrid waves. These waves lead to the transport of high‐density magnetosheath plasma across the boundary layer into the magnetosphere and generate a mixing region with highly compressed magnetic field lines, leading to the formation of a thin current sheet associated with electron‐scale reconnection signatures. Consistencies between these reconnection signatures and a realistic, local, fully‐kinetic simulation modeling this current sheet indicate a temporal evolution of the observed electron‐scale reconnection current sheet. The multi‐scale and inter‐process character of this event can help us understand plasma mixing connected to the Kelvin‐Helmholtz instability and the temporal evolution of electron‐scale reconnection.

As a result of the large difference in heat capacity between land and ocean, global climate and atmospheric circulation patterns over the supercontinent of Pangaea were significantly different from those of today. Modelling experiments indicate a seasonal alternation in cross-equatorial flow induced by the seasonal reversal in the direction of the monsoonal circulation; however, there are large discrepancies between model-generated surface wind patterns and the reported palaeowind directions from aeolian dune records. Here, we present the spatial distribution of dune slip-face azimuths recorded in the Lower Jurassic aeolian sandstones over a wide area of the western United States (palaeolatitude: ∼19–27∘ N). The azimuth data for dune slip faces reveal a bidirectional and oblique angular pattern that resembles the internal structures of modern longitudinal dunes. Based on the spatial pattern of slip-face directions and outcrop evidence, we suggest that most Lower Jurassic aeolian sandstones were NNE–SSW- to NNW–SSE-oriented longitudinal dunes, which likely formed as the result of a combination of westerly, northwesterly, and northeasterly palaeowinds. The reconstructed palaeowind pattern at ∼19–27∘ N appears to be consistent with the model-generated surface wind pattern and its seasonal alternation. The reconstructed palaeowind patterns also suggest an influence of orbitally induced changes in atmospheric pressure patterns over Pangaea.

Resuspension of 137Cs from the contaminated ground surface to the atmosphere is essential for understanding the environmental behaviors of 137Cs and estimating external and inhalation exposure of residents. Kajino et al. (2016) assessed the 137Cs resuspension flux from bare soil and forest ecosystems in eastern Japan in 2013 using a numerical simulation constrained by surface air concentration measurements. However, the simulation was found to underestimate the observed deposition amounts by 2 orders of magnitude. The reason for this underestimation is that the simulation assumed that resuspended 137Cs is carried by submicron aerosols, which have low deposition rates. Based on the observational indications that soil dust and bioaerosols are the major carriers of resuspended 137Cs, a new simulation is performed with higher deposition rates constrained by both surface concentrations and deposition amounts. In the new estimation, the total areal annual resuspension of 137Cs in 2013 is 25.7 TBq, which is equivalent to 0.96 % of the initial deposition (2.68 PBq). Due to the rapid deposition rates, the annual redeposition amount is also large at 10.6 TBq, approximately 40 % of the resuspended 137Cs. The resuspension rate through the atmosphere (0.96 % yr−1) seems slow, but it (2.6 × 10−5 d−1) may not be negligibly small compared to the actual decreasing trend of the ambient gamma dose rate obtained in Fukushima Prefecture after the radioactive decay of 137Cs plus 134Cs in 2013 is subtracted (1.0–7.9 × 10−4 d−1): resuspension can account for 1 %–10 % of the decreasing rate due to decontamination and natural decay through land surface processes. The current simulation underestimated the 137Cs deposition in Fukushima city in winter by more than an order of magnitude, indicating the presence of additional resuspension sources. The site of Fukushima city is surrounded by major roads. Heavy traffic on wet and muddy roads after snow removal operations could generate superlarge (approximately 100 µm in diameter) road dust or road salt particles, which are not included in the model but might contribute to the observed 137Cs at the site.

Background Although R0 surgery is recommended for stage IV colorectal cancer, the degree of required lymphadenectomy has not been established. The aim of this study was to investigate the prognostic impact of high ligation (HL) of the feeding artery and the number of retrieved lymph nodes after R0 surgery for colorectal cancer and synchronous colorectal cancer liver metastasis (CRLM). Methods This was a multi‐institutional retrospective analysis of patients with colorectal cancer and synchronous CRLM who had R0 surgery between January 1997 and December 2007. Clinical and pathological features were compared in patients who underwent HL and those who had a low ligation (LL). Kaplan–Meier analysis was performed to estimate the effect of HL on overall survival (OS). The impact of several risk factors on survival was analysed using the Cox proportional hazards model. Results Of 549 patients, 409 (74·5 per cent) had HL. Median follow‐up was 51·4 months. HL significantly improved the 5‐year OS rate (58·2 per cent versus 49·3 per cent for LL; P = 0·017). Multivariable analysis revealed HL to be a significant prognostic factor compared with LL (5‐year mortality: hazard ratio (HR) 0·68, 95 per cent c.i. 0·51 to 0·90; P = 0·007). In subgroup analysis, the positive effect of HL on OS was greatest in patients with lymph node metastasis. Conclusion HL of the feeding artery was associated with improved OS in patients with colorectal cancer and synchronous CRLM after R0 surgery.

Zebrafish are a powerful animal model for small molecule screening. Small molecule treatments of zebrafish embryos usually require that the chorion, an acellular envelope enclosing the embryo, is removed in order for chemical compounds to access the embryo from the bath medium. For large-scale studies requiring hundreds of embryos, manual dechorionation, using forceps, can be a time-consuming and limiting process. Pronase is a non-specific protease that is widely used as an enzymatic alternative for dechorionating zebrafish embryos. However, whether pronase treatments alter the effects of subsequent small molecule treatments has not been addressed. Here, we provide a detailed protocol for large-scale pronase dechorionation of zebrafish embryos. We tested whether pronase treatment can influence the efficacy of drug treatments in zebrafish embryos. We used a zebrafish model for Duchenne muscular dystrophy (DMD) to investigate whether the efficacies of trichostatin-A (TSA) or salermide + oxamflatin, small molecule inhibitors known to ameliorate the zebrafish dmd muscle degeneration phenotype, are significantly altered when embryos are treated with pronase versus manual dechorionation. We also tested the effects of pronase on the ability of the anthracycline cancer drug doxorubicin to induce cardiotoxicity in zebrafish embryos. When comparing pronase- versus forceps-dechorionated embryos used in these small molecule treatments, we found no appreciable effects of pronase on animal survival or on the effects of the small molecules. The significant difference that was detected was a small improvement in the ability of salermide + oxamflatin to ameliorate the dmd phenotype in pronase-treated embryos when compared with manual dechorionation. Our study supports the use of pronase treatment as a dechorionation method for zebrafish drug screening experiments.

This research numerically studies the formation of a detached plasma and the energy loss processes during hydrogen (H) gas puffing by using the 1D-3 V Particle-In-Cell (PIC) code. In this study, the 1D-3 V PIC with Monte Carlo Collision (MCC) and cumulative scattering angle Coulomb collision has been developed for investigating the kinetic behavior of the detached plasma. The plasma density increases near the target plate with increasing neutral pressure. The flow velocity, the ion temperature, and the electron temperature are reduced toward the target plate when gas puffing is performed by enhancing the charge-exchange (CX), the ionization, and the excitation loss. For the 15 mTorr neutral pressure case, all of the Te||, Te⊥, Ti||, and Ti⊥ reduce to less than 1 eV. The heat flux on the target plate reduces with the increment of neutral pressure. The heat flux is remarkably suppressed for the 15 mTorr neutral pressure. The sheath and the presheath potential drop on the target are affected with changing neutral pressure. The power loss terms are enhanced according to the increase of H neutral pressure. For all cases, the CX loss is shown to be higher than 70%. The excitation loss and ionization loss are shown to be nearly 15% and 6%, respectively. The recombination loss is found to be small, but it increases with increasing neutral pressure.

The westerly wind travelling at high altitudes over eastern Asia transports aerosols from the Asian deserts and urban areas to downwind areas such as Japan. These long-range-transported aerosols include not only mineral particles but also microbial particles (bioaerosols), that impact the ice-cloud formation processes as ice nuclei. However, the detailed relations of airborne bacterial dynamics to ice nucleation in high-elevation aerosols have not been investigated. Here, we used the aerosol particles captured in the snow cover at altitudes of 2450 m on Mt Tateyama to investigate sequential changes in the ice-nucleation activities and bacterial communities in aerosols and elucidate the relationships between the two processes. After stratification of the snow layers formed on the walls of a snow pit on Mt Tateyama, snow samples, including aerosol particles, were collected from 70 layers at the lower (winter accumulation) and upper (spring accumulation) parts of the snow wall. The aerosols recorded in the lower parts mainly came from Siberia (Russia), northern Asia and the Sea of Japan, whereas those in the upper parts showed an increase in Asian dust particles originating from the desert regions and industrial coasts of Asia. The snow samples exhibited high levels of ice nucleation corresponding to the increase in Asian dust particles. Amplicon sequencing analysis using 16S rRNA genes revealed that the bacterial communities in the snow samples predominately included plant associated and marine bacteria (phyla Proteobacteria) during winter, whereas during spring, when dust events arrived frequently, the majority were terrestrial bacteria of phyla Actinobacteria and Firmicutes. The relative abundances of Firmicutes (Bacilli) showed a significant positive relationship with the ice nucleation in snow samples. Presumably, Asian dust events change the airborne bacterial communities over Mt Tateyama and carry terrestrial bacterial populations, which possibly induce ice-nucleation activities, thereby indirectly impacting climate change.

Vortex-induced reconnection originates from non-linear vortex flows due to Kelvin-Helmholtz instability in the Earth’s magnetosphere. Here, the authors perform a large-scale kinetic simulation to unveil dynamics of the vortex-induced reconnection and resulting turbulent mixing process.

Aerosol particles, including airborne microorganisms, are transported through the free troposphere from the Asian continental area to the downwind area in East Asia and can influence climate changes, ecosystem dynamics, and human health. However, the variations present in airborne bacterial communities in the free troposphere over downwind areas are poorly understood, and there are few studies that provide an in-depth examination of the effects of long-range transport of aerosols (natural and anthropogenic particles) on bacterial variations. In this study, the vertical distributions of airborne bacterial communities at high altitudes were investigated and the bacterial variations were compared between dust events and non-dust events.Aerosols were collected at three altitudes from ground level to the free troposphere (upper level: 3000 or 2500 m; middle level: 1200 or 500 m; and low level: 10 m) during Asian dust events and non-dust events over the Noto Peninsula, Japan, where westerly winds carry aerosols from the Asian continental areas. During Asian dust events, air masses at high altitudes were transported from the Asian continental area by westerly winds, and laser imaging detection and ranging (lidar) data indicated high concentrations of non-spherical particles, suggesting that dust-sand particles were transported from the central desert regions of Asia. The air samples collected during the dust events contained 10–100 times higher concentrations of microscopic fluorescent particles and optical particle counter (OPC) measured particles than in non-dust events. The air masses of non-dust events contained lower amounts of dust-sand particles. Additionally, some air samples showed relatively high levels of black carbon, which were likely transported from the Asian continental coasts. Moreover, during the dust events, microbial particles at altitudes of > 1200 m increased to the concentrations ranging from 1. 2 × 106 to 6. 6 × 106 particles m−3. In contrast, when dust events disappeared, the microbial particles at > 1200 m decreased slightly to microbial-particle concentrations ranging from 6. 4 × 104 to 8. 9 × 105 particles m−3.High-throughput sequencing technology targeting 16S rRNA genes (16S rDNA) revealed that the bacterial communities collected at high altitudes (from 500 to 3000 m) during dust events exhibited higher diversities and were predominantly composed of natural-sand/terrestrial bacteria, such as Bacillus members. During non-dust periods, airborne bacteria at high altitudes were mainly composed of anthropogenic/terrestrial bacteria (Actinobacteria), marine bacteria (Cyanobacteria and Alphaproteobacteria), and plant-associated bacteria (Gammaproteobacteria), which shifted in composition in correspondence with the origins of the air masses and the meteorological conditions. The airborne bacterial structures at high altitudes suggested remarkable changes in response to air mass sources, which contributed to the increases in community richness and to the domination of a few bacterial taxa.

We present the first results of a data analysis method, developed by Sonnerup and Hasegawa (2011), for reconstructing three-dimensional (3-D), magnetohydrostatic structures from data taken as two closely spaced satellites traverse the structures. The method is applied to a magnetic flux transfer event (FTE), which was encountered on 27 June 2007 by at least three (TH-C, TH-D, and TH-E) of the five THEMIS probes near the subsolar magnetopause. The FTE was sandwiched between two oppositely directed reconnection jets under a southward interplanetary magnetic field condition, consistent with its generation by multiple X-line reconnection. The recovered 3-D field indicates that a magnetic flux rope with a diameter of ~ 3000 km was embedded in the magnetopause. The FTE flux rope had a significant 3-D structure, because the 3-D field reconstructed from the data from TH-C and TH-D (separated by ~ 390 km) better predicts magnetic field variations actually measured along the TH-E path than does the 2-D Grad–Shafranov reconstruction using the data from TH-C (which was closer to TH-E than TH-D and was at ~ 1250 km from TH-E). Such a 3-D nature suggests that the field lines reconnected at the two X-lines on both sides of the flux rope are entangled in a complicated way through their interaction with each other. The generation process of the observed 3-D flux rope is discussed on the basis of the reconstruction results and the pitch-angle distribution of electrons observed in and around the FTE.

Understanding the behavior of the global climate system during extremely warm periods is one of the major themes of paleoclimatology. Proxy data demonstrate that the equator-to-pole temperature gradient was much lower during the mid-Cretaceous "supergreenhouse" period than at present, implying larger meridional heat transport by atmospheric and/or oceanic circulation. However, reconstructions of atmospheric circulation during the Cretaceous have been hampered by a lack of appropriate datasets based on reliable proxies. Desert distribution directly reflects the position of the subtropical high-pressure belt, and the prevailing surface-wind pattern preserved in desert deposits reveals the exact position of its divergence axis, which marks the poleward margin of the Hadley circulation. We reconstructed temporal changes in the latitude of the subtropical high-pressure belt and its divergence axis during the Cretaceous based on spatio-temporal changes in the latitudinal distribution of deserts and prevailing surface-wind patterns in the Asian interior. We found a poleward shift in the subtropical high-pressure belt during the early and late Cretaceous, suggesting a poleward expansion of the Hadley circulation. In contrast, an equatorward shift of the belt was found during the mid-Cretaceous "supergreenhouse" period, suggesting drastic shrinking of the Hadley circulation. These results, in conjunction with recent observations, suggest the existence of a threshold in atmospheric CO2 level and/or global temperature, beyond which the Hadley circulation shrinks drastically.

The Kelvin-Helmholtz Instability (KHI) can drive waves at the magnetopause. These waves can grow to form rolled-up vortices and facilitate transfer of plasma into the magnetosphere. To investigate the persistence and frequency of such waves at the magnetopause we have carried out a survey of all Double Star 1 magnetopause crossings, using a combination of ion and magnetic field measurements. Using criteria originally used in a Geotail study made by Hasegawa et al. (2006) (forthwith referred to as H2006), 17 candidate events were identified from the entire TC-1 mission (covering ~623 orbits where the magnetopause was sampled), a majority of which were on the dayside of the terminator. The relationship between density and shear velocity was then investigated, to identify the predicted signature of a rolled up vortex from H2006 and all 17 events exhibited some level of rolled up behavior. The location of the events had a clear dawn-dusk asymmetry, with 12 (71%) on the post noon, dusk flank suggesting preferential growth in this region.

A study of the interaction of solar wind magnetic field rotations with the Earth's magnetosphere is performed. For this event there is, for the first time, a full coverage over the dayside magnetosphere with multiple (multi)spacecraft missions from dawn to dusk, combined with ground magnetometers, radar and an auroral camera, this gives a unique coverage of the response of the Earth's magnetosphere. After a long period of southward IMF Bz and high dynamic pressure of the solar wind, the Earth's magnetosphere is eroded and compressed and reacts quickly to the turning of the magnetic field. We use data from the solar wind monitors ACE and Wind and from magnetospheric missions Cluster, THEMIS, DoubleStar and Geotail to investigate the behaviour of the magnetic rotations as they move through the bow shock and magnetosheath. The response of the magnetosphere is investigated through ground magnetometers and auroral keograms. It is found that the solar wind magnetic field drapes over the magnetopause, while still co-moving with the plasma flow at the flanks. The magnetopause reacts quickly to IMF Bz changes, setting up field aligned currents, poleward moving aurorae and strong ionospheric convection. Timing of the structures between the solar wind, magnetosheath and the ground shows that the advection time of the structures, using the solar wind velocity, correlates well with the timing differences between the spacecraft. The reaction time of the magnetopause and the ionospheric current systems to changes in the magnetosheath Bz seem to be almost immediate, allowing for the advection of the structure measured by the spacecraft closest to the magnetopause.

During April to July 2007 a combination of 10 spacecraft provided simultaneous monitoring of the dayside magnetopause across a wide range of local times. The array of four Cluster spacecraft, separated at large distances (10 000 km), were traversing the dawn-side magnetopause at high and low latitudes; the five THEMIS spacecraft were often in a 4 + 1 grouped configuration, traversing the low latitude, dusk-side magnetosphere, and the Double star, TC-1 spacecraft was in an equatorial orbit between the local times of the THEMIS and Cluster orbits. We show here a number of near simultaneous conjunctions of all 10 spacecraft at the magnetopause. One conjunction identifies an extended magnetic reconnection X-line, tilted in the low latitude, sub-solar region, which exists together with active anti-parallel reconnection sites extending to locations on the dawn-side flank. Oppositely moving FTE's are observed on all spacecraft, consistent with the initially strong IMF By conditions and the comparative locations of the spacecraft both dusk-ward and dawn-ward of noon. Comparison with other conjunctions of magnetopause crossings, which are also distributed over wide local times, supports the result that reconnection activity may occur at many sites simultaneously across the sub-solar and flank magnetopause, but linked to the large scale (extended) configuration of the merging line; broadly depending on IMF orientation. The occurrence of MR therefore inherently follows a "component" driven scenario irrespective of the guide field conditions. Some conjunctions allow the global magnetopause response to IMF changes to be observed and the distribution of spacecraft can directly confirm its shape, motion and deformation at local noon, dawn and dusk-side, simultaneously.

During a storm recovery phase on 15 May 2005, the Geotail spacecraft repeatedly observed high-energy (>180 keV) oxygen ions in the dayside magnetosheath near the equatorial plane. We focused on the time period from 11:20 UT to 13:00 UT, when Geotail observed the oxygen ions and the interplanetary magnetic field (IMF) was constantly northward. The magnetic reconnection occurrence northward and duskward of Geotail is indicated by the Walén analysis and convective flows in the magnetopause boundary layer. Anisotropic pitch angle distributions of ions suggest that high-energy oxygen ions escaped from the northward of Geotail along the reconnected magnetic field lines. From the low-energy particle precipitation in the polar cap observed by DMSP, which is consistent with magnetic reconnection occurring between the magnetosheath field lines and the magnetospheric closed field lines, we conclude that these oxygen ions are of ring current origin. Our results thus suggest a new escape route of oxygen ions during northward IMF. In the present event, this escape mechanism is more dominant than the leakage via the finite Larmor radius effect across the dayside equatorial magnetopause.

Recently, Keiling et al. (2006) showed that periodic (~90 s) traveling compression regions (TCRs) during a substorm had properties of Pi2 pulsations, prompting them to call this type of periodic TCRs "lobe Pi2". It was further shown that time-delayed ground Pi2 had the same period as the lobe Pi2 located at 16 RE, and it was concluded that both were remotely driven by periodic, pulsed reconnection in the magnetotail. In the study reported here, we give further evidence for this association by reporting additional periodic TCR events (lobe Pi2s) at 18 RE all of which occurred in succession during a geomagnetically very quiet, non-substorm period. Each quiet-time periodic TCR event occurred during an interval of small H-bay-like ground disturbance (H bays were superposed by Pi2s. These ground Pi2s are compared to the TCRs in the tail lobe (Cluster) and both magnetic pulsations and flow variations at 9 RE inside the plasma sheet (Geotail). The main results of this study are: (1) Further evidence is given that periodic TCRs in the tail lobe at distances of 18 RE and ground Pi2 are related phenomena. In particular, it is shown that both had the same periodicity and occurred simultaneously (allowing for propagation time delays) strongly suggesting that both had the same periodic source. Since the TCRs were propagating Earthward, this source was located in the outer magnetosphere beyond 18 RE. (2) The connection of periodic TCRs and ground Pi2 also exists during very quiet geomagnetic conditions with PBIs present in addition to the previous result (Keiling et al., 2006) which showed this connection during substorms. (3) Combining (1) and (2), we conclude that the frequency of PBI-associated Pi2 is controlled in the outer magnetosphere as opposed to the inner magnetosphere. We propose that this mechanism is pulsed reconnection based on previous results which combined modeled results and observations of substorm-related periodic TCRs and ground Pi2. (4) We show that TCRs with small compression ratios (ΔB/B1% (which are more commonly studied). (5) Finally, it is noted that both quiet time and substorm-related periodic TCRs had remarkably similar periods in spite of the drastically different geomagnetic conditions prevailing during the events which poses the important question of what causes this periodicity under these different conditions.

Many phenomena in the Earth's magnetotail have characteristic temporal scales of several minutes and spatial scales of a few Earth radii (RE). Examples of such transient and localized mesoscale phenomena are bursty bulk flows, beamlets, energy dispersed ion beams, flux ropes, traveling compression regions, night-side flux transfer events, and rapid flappings of the current sheet. Although most of these observations are linked to specific interpretations or theoretical models they are inter-related and can be the different aspects of a physical process or origin. Recognizing the inter-connected nature of the different transient and localized phenomena in the magnetotail, this paper reviews their observations by highlighting their important characteristics, with emphasis on the new results from Cluster multipoint observations. The multi-point Cluster measurements have provided, for the first time, the ability to distinguish between temporal and spatial variations, and to resolve spatial structures. Some examples of the new results are: flux ropes with widths of 0.3 RE, transient field aligned currents associated with bursty bulk flows and connected to the Hall current at the magnetic reconnection, flappings of the magnetotail current sheet with time scales of 100 s–10 min and thickness of few thousand km, and particle energization including velocity and time dispersed ion structures with the latter having durations of 1–3 min. The current theories of these transient and localized processes are based largely on magnetic reconnection, although the important role of the interchange and other plasma modes are now well recognized. On the kinetic scale, the energization of particles takes place near the magnetic X-point by non-adiabatic processes and wave-particle interactions. The theory, modeling and simulations of the plasma and field signatures are reviewed and the links among the different observational concepts and the theoretical frameworks are discussed. The mesoscale processes in the magnetotail and the strong coupling among them are crucial in developing a comprehensive understanding of the multiscale phenomena of the magnetosphere.

Flow separation is mostly an undesirable phenomenon and boundary layer control is an important technique for flow separation problems on airfoils and in diffusers. Longitudinal (streamwise) vortices are produced by the interaction between jets and a freestream. This technique is known as the vortex generator jet method of separation, or stall control. The vortex generator jet method is an active control technique that provides a time-varying control action to optimize performance under a wide range of flow conditions because the strength of longitudinal vortices can be adjusted by varying the jet speed. In the present study, an active separation control system using vortex generator jets with rectangular orifices has been developed. The active separation control system can be practically applied to the flow separation control of a two-dimensional diffuser. It was confirmed that the proposed active separation control system could adaptively suppress flow separation for the flow fields caused by some changes in freestream velocity and the divergence angle of the diffuser.

This article describes observations of a bursty bulk flow (BBF) in the outer central plasma sheet. The observations are made with the Cluster satellites, located approximately 19 RE downtail, close to the midnight sector in the Southern Hemisphere. 40–60 s after Cluster first detected the BBF, there was a large bipolar perturbation in the magnetic field. A Grad-Shafranov reconstruction has revealed that this is created by a field-aligned current at the flank of the BBF. Further analysis of the plasma moments has shown that the BBF has the properties of a depleted flux tube. Depleted flux tubes are an important theoretical model for how plasma and magnetic flux can be transported Earthward in the magnetotail as part of the Dungey cycle. The field aligned current is directed Earthward and is located at the dawn side of the BBF. Thus, it is consistent with the magnetic shear at the flank of an Earthward moving BBF. The total current has been estimated to be about 0.1 MA.

The structure and formation mechanism of a total of five Flux Transfer Events (FTEs), encountered on the equatorward side of the northern cusp by the Cluster spacecraft, with separation of ~5000 km, are studied by applying the Grad-Shafranov (GS) reconstruction technique to the events. The technique generates a magnetic field/plasma map of the FTE cross section, using combined magnetic field and plasma data from all four spacecraft, under the assumption that the structure is two-dimensional (2-D) and time-independent. The reconstructed FTEs consist of one or more magnetic flux ropes embedded in the magnetopause, suggesting that multiple X-line reconnection was involved in generating the observed FTEs. The dimension of the flux ropes in the direction normal to the magnetopause ranges from about 2000 km to more than 1 RE. The orientation of the flux rope axis can be determined through optimization of the GS map, the result being consistent with those from various single-spacecraft methods. Thanks to this, the unambiguous presence of a strong core field is confirmed, providing evidence for component merging. The amount of magnetic flux contained within each flux rope is calculated from the map and, by dividing it by the time interval between the preceding FTE and the one reconstructed, a lower limit of the reconnection electric field during the creation of the flux rope can be estimated; the estimated value ranges from ~0.11 to ~0.26 mV m-1, with an average of 0.19 mV m-1. This can be translated to the reconnection rate of 0.038 to 0.074, with an average of 0.056. Based on the success of the 2-D model in recovering the observed FTEs, the length of the X-lines is estimated to be at least a few RE.

Since December 2000, the Cluster satellites have been conducting detailed measurements of the magnetospheric boundaries and have confirmed the unambiguous presence of ions of terrestrial origin (e.g. O+ in regions adjacent to the dayside, mid-latitude magnetopause. In the present paper, we focus on the statistical properties of the O+ ion component at energies ranging from 30eV up to 40keV, using three years of ion data at solar maximum from the Cluster Ion Spectrometry (CIS) experiment aboard two Cluster spacecraft. The O+ density decreases on average by a factor of 6, from 0.041 to 7x10-3cm-3 when crossing the magnetopause from the magnetosphere to the magnetosheath, but depends on several parameters, such as the geomagnetic activity or the modified disturbed storm time index (Dst*), and on their location. The O+ density is significantly higher in the dusk-side than in the dawn side region, which is consistent with the view that they originate mainly from the plasma sheet. A remarkable finding is that inward of the magnetopause, O+ is the dominant contributor to the mass density 30% of the time on the dusk-side in comparison to 3% in the dawnside and 4% near noon. On an event basis in the dusk flank of the magnetopause, we point out that O+ ions, when dominating the mass composition, lower the threshold for generating the Kelvin-Helmholtz instability, which may allow plasma exchange between the magnetosheath and the plasma sheet. We also discuss the effect of a substantial O+ ion component when present in a reconnection region.

The paper discusses properties of the near-tail dawnside and boundary layer, as obtained from Cluster plasma and magnetic field measurements during a single skimming orbit on 4 and 5 July 2001 that included 24 well-defined crossings by all four spacecraft. As a result of variations of the interplanetary magnetic field, the magnetic shear across the local varied between ~0° and ~180°. Using an improved method, which takes into account acceleration and thickness variation, we have determined the orientation, speed, thickness and current for the 96 individual crossings. The orientations show clear evidence of surface waves. Magnetopause thicknesses range from ~100 to ~2500km, with an average of 753km. The speeds range from less than 10up to more than 300, with an average of 48. Both results are consistent with earlier ISEE and AMPTE results obtained for the dayside magnetopause. Importantly, scaling the thicknesses to the ion gyro radius or the ion inertial length did not reduce the large dynamic range. There is also no significant dependence of thickness on magnetic shear. Current densities range from ~0.01 up to ~0.3uA, with an average value of 0.05 . By including some extra crossings that did not involve all four spacecraft, we were able to apply the Walén test to a total of 60 by Cluster 1 and 3, and have classified 19 cases as rotational discontinuities (RDs), of which 12 and 7 were sunward and tailward of an X-line, respectively. Of these 60 crossings, 26 show no trace of a boundary layer. The only with substantial boundary layers are into the plasma mantle. Of the 26 without a boundary layer, 8 were identified as RDs. Since reconnection produces wedge-shaped boundary layers emanating from the X-line, RDs without may be considered close to the X-line, in which case the observed magnetic shear and Alfvén Mach number should be representative of the conditions at the X-line itself. It is therefore important that four of the eight cases had shear angles ≤100, i.e. the reconnecting fields were far from being anti-parallel, and that all eight cases had Alfvén Mach numbers MA>1 in the adjoining magnetosheath. Another important conclusion can be drawn from the without a that were tangential discontinuities (TDs). To observe TDs with no at such large distances from the subsolar point appears to rule out diffusion over large portions of the as an effective means for plasma transport across the magnetopause.

The Grad-Shafranov (GS) reconstruction technique, a single-spacecraft based data analysis method for recovering approximately two-dimensional (2-D) magnetohydrostatic plasma/field structures in space, is improved to become a multi-spacecraft technique that produces a single field map by ingesting data from all four Cluster spacecraft into the calculation. The plasma pressure, required for the technique, is measured in high time resolution by only two of the spacecraft, C1 and C3, but, with the help of spacecraft potential measurements available from all four spacecraft, the pressure can be estimated at the other spacecraft as well via a relationship, established from C1 and C3 data, between the pressure and the electron density deduced from the potentials. Consequently, four independent field maps, one for each spacecraft, can be reconstructed and then merged into a single map. The resulting map appears more accurate than the individual single-spacecraft based ones, in the sense that agreement between magnetic field variations predicted from the map to occur at each of the four spacecraft and those actually measured is significantly better. Such a composite map does not satisfy the GS equation any more, but is optimal under the constraints that the structures are 2-D and time-independent. Based on the reconstruction results, we show that, even on a scale of a few thousand km, the magnetopause surface is usually not planar, but has significant curvature, often with intriguing meso-scale structures embedded in the current layer, and that the thickness of both the current layer and the boundary layer attached to its earthward side can occasionally be larger than 3000km.

In this paper, we use Cluster data from one magnetopause event on 5 July 2001 to compare predictions from various methods for determination of the velocity, orientation, and thickness of the magnetopause current layer. We employ established as well as new multi-spacecraft techniques, in which time differences between the crossings by the four spacecraft, along with the duration of each crossing, are used to calculate magnetopause speed, normal vector, and width. The timing is based on data from either the Cluster Magnetic Field Experiment (FGM) or the Electric Field Experiment (EFW) instruments. The multi-spacecraft results are compared with those derived from various single-spacecraft techniques, including minimum-variance analysis of the magnetic field and deHoffmann-Teller, as well as Minimum-Faraday-Residue analysis of plasma velocities and magnetic fields measured during the crossings. In order to improve the overall consistency between multi- and single-spacecraft results, we have also explored the use of hybrid techniques, in which timing information from the four spacecraft is combined with certain limited results from single-spacecraft methods, the remaining results being left for consistency checks. The results show good agreement between magnetopause orientations derived from appropriately chosen single-spacecraft techniques and those obtained from multi-spacecraft timing. The agreement between magnetopause speeds derived from single- and multi-spacecraft methods is quantitatively somewhat less good but it is evident that the speed can change substantially from one crossing to the next within an event. The magnetopause thickness varied substantially from one crossing to the next, within an event. It ranged from 5 to 10 ion gyroradii. The density profile was sharper than the magnetic profile: most of the density change occured in the earthward half of the magnetopause. Key words. Magnetospheric physics (magnetopause, cusp and boundary layers; instruments and techniques) – Space plasma physics (discontinuities)

A recently developed technique for reconstructing approximately two-dimensional (∂/∂z≈0), time-stationary magnetic field structures in space is applied to two magnetopause traversals on the dawnside flank by the four Cluster spacecraft, when the spacecraft separation was about 2000km. The method consists of solving the Grad-Shafranov equation for magnetohydrostatic structures, using plasma and magnetic field data measured along a single spacecraft trajectory as spatial initial values. We assess the usefulness of this single-spacecraft-based technique by comparing the magnetic field maps produced from one spacecraft with the field vectors that other spacecraft actually observed. For an optimally selected invariant (z)-axis, the correlation between the field components predicted from the reconstructed map and the corresponding measured components reaches more than 0.97. This result indicates that the reconstruction technique predicts conditions at the other spacecraft locations quite well. The optimal invariant axis is relatively close to the intermediate variance direction, computed from minimum variance analysis of the measured magnetic field, and is generally well determined with respect to rotations about the maximum variance direction but less well with respect to rotations about the minimum variance direction. In one of the events, field maps recovered individually for two of the spacecraft, which crossed the magnetopause with an interval of a few tens of seconds, show substantial differences in configuration. By comparing these field maps, time evolution of the magnetopause structures, such as the formation of magnetic islands, motion of the structures, and thickening of the magnetopause current layer, is discussed. Key words. Magnetospheric physics (Magnetopause, cusp, and boundary layers) – Space plasma physics (Experimental and mathematical techniques, Magnetic reconnection)

The phospholipids from murine mastocytoma FMA3 and P-815 clone cells were quantitatively analyzed, and the major glycerophospholipids were examined for their fatty acyl chain distribution. In these cells, the content of histamine was less than 1/100 of normal mouse mast cells, and FMA3 cells had 1.5-fold as much histamine content as P-815 cells. The predominant phospholipid species of both mastocytoma FMA3 and P-815 were choline-containing glycerophospholipids (48%) and ethanolamine-containing glycerophospholipids (29%). The remaining minor constituents were sphingomyelin (6%, 7%), phosphatidylinositol (7%, 5%), phosphatidylserine (2%, 5%), cardiolipin (4%, 3%), and phosphatidic acid (2%, 1% for FMA3 and P-815, respectively). The choline-containing glycerophospholipids consisted of high amounts of 1-O-alkyl-2-acyl type (31%, 25%) and 1,2-diacyl type (63%, 66%) and a smaller amount of 1-O-alk-1'-enyl-2-acyl type (7%, 8%). In contrast, ethanolamine-containing glycerophospholipids were characterized by high contents of 1-O-alk-1'-enyl-2-acyl type (36%, 31%) and 1,2-diacyl type (55%, 58%), and a lower level of 1-O-alkyl-2-acyl type (12% and 11% for FMA3 and P-815, respectively). Unlike choline-containing glycerophospholipids and sphingomyelin that were rich in palmitic acid, ethanolamine-containing glycerophospholipids, phosphatidylserine and phosphatidylinositol showed a high proportion of stearic acid in the overall fatty acid composition. The content of arachidonic acid was highest in phosphatidylinositol. Sphingomyelin had a large amount of long chain and polyunsaturated fatty acids. In both choline- and ethanolamine-containing glycerophospholipids, the predominant fatty acids in the sn-1-position were palmitic, stearic, and oleic acid.(ABSTRACT TRUNCATED AT 250 WORDS)

This study was aimed at identifying a quantitatively accurate reaction model of the bromate−sulfilte−ferrocyanide (BSF) pH oscillator by using the simulation-based model estimation algorithm known as the particle filter. The Rábai−Kaminaga−Hanazaki (RKH) model proposed for the BSF system was extended by adding the protonation equilibrium of SO42−, for which the particle filter analysis was carried out to optimize the rate constants involved with reference to the measured pH oscillation data. The extended RKH model with the optimized rate constants almost completely reproduced the measured pH oscillations and the state diagram, showing the validity of the present analysis. Chemical oscillators such as the BSF system show drastic switching of the dominant reaction path, which strongly disturbs the convergence of the rate constants if the objective function is defined in a conventional manner to reflect only a single time step datum. In this study, the objective function was defined as the residual sum of squares with respect to pH taken over an interval longer than one oscillatory period, so that all of the relevant reaction steps can contribute to the objective function. This is the first report which exemplifies the effectiveness of the particle filter in the analysis of real complex chemical systems. [ABSTRACT FROM AUTHOR]

Small animal SPECT imaging systems have multiple potential applications in biomedical research. Whereas SPECT data are commonly interpreted qualitatively in a clinical setting, the ability to accurately quantify measurements will increase the utility of the SPECT data for laboratory measurements involving small animals. In this work, we assess the effect of photon attenuation, scatter and partial volume errors on the quantitative accuracy of small animal SPECT measurements, first with Monte Carlo simulation and then confirmed with experimental measurements. The simulations modeled the imaging geometry of a commercially available small animal SPECT system. We simulated the imaging of a radioactive source within a cylinder of water, and reconstructed the projection data using iterative reconstruction algorithms. The size of the source and the size of the surrounding cylinder were varied to evaluate the effects of photon attenuation and scatter on quantitative accuracy. We found that photon attenuation can reduce the measured concentration of radioactivity in a volume of interest in the center of a rat-sized cylinder of water by up to 50% when imaging with iodine-125, and up to 25% when imaging with technetium-99m. When imaging with iodine-125, the scatter-to-primary ratio can reach up to approximately 30%, and can cause overestimation of the radioactivity concentration when reconstructing data with attenuation correction. We varied the size of the source to evaluate partial volume errors, which we found to be a strong function of the size of the volume of interest and the spatial resolution. These errors can result in large (>50%) changes in the measured amount of radioactivity. The simulation results were compared with and found to agree with experimental measurements. The inclusion of attenuation correction in the reconstruction algorithm improved quantitative accuracy. We also found that an improvement of the spatial resolution through the use of resolution recovery techniques (i.e. modeling the finite collimator spatial resolution in iterative reconstruction algorithms) can significantly reduce the partial volume errors. [ABSTRACT FROM AUTHOR]

Computed tomography (CT) remains a critical diagnostic tool for evaluating patients with cerebrovascular disease, and the advent of specialized systems for imaging rodents has extended these techniques to small animal models of these diseases. We therefore have evaluated in vivo methods of imaging rat models of hemorrhagic stroke using a high resolution compact computed tomography (''microCT'') system (FLEX(tm) X-O(tm), Gamma Medica-Ideas, Northridge, CA). For all in vivo studies, the head of the anesthetized rat was secured in a custom immobilization device for microCT imaging with 512 projections over 2 min at 60 kVp and 0.530 mA (Itube × t/rotation = 63.6 mAs). First, imaging without iodinated contrast was performed (a) to differentiate the effect of contrast agent in contrast-enhanced CT and (b) to examine the effectiveness of the immobilization device between two time points of CT acquisitions. Then, contrast-enhanced CT was performed with continuous administration of iopromide (300 mgI ml[?]1 at 1.2 ml min[?]1) to visualize aneurysms and other vascular formations in the carotid and cerebral arteries that may precede subarachnoid hemorrhage. The accuracy of registration between the noncontrast and contrast-enhanced CT images with the immobilization device was compared against the images aligned with normalized mutual information using FMRIB''s linear image registration tool (FLIRT). Translations and rotations were examined between the FLIRT-aligned noncontrast CT image and the nonaligned noncontrast CT image. These two data sets demonstrated translational and rotational differences of less than 0.5 voxel ([?]85 µm) and 0.5°, respectively. Noncontrast CT demonstrated a very small volume (0.1 ml) of femoral arterial blood introduced surgically into the rodent brain. Continuous administration of iopromide during the CT acquisition produced consistent vascular contrast in the reconstructed CT images. As a result, carotid arteries and major cerebral blood vessels were visible with contrast-enhanced CT, but not with noncontrast CT. In conclusion, the CT-compatible immobilization device was useful for in vivo microCT imaging of intracranial blood and of vascular structures within and immediately adjacent to the rodent brain. The microCT imaging technique is also compatible with continuous administration of a conventional iodinated contrast agent (e.g. iopromide) and therefore does not require specialized small animal specific contrast agent that has comparatively long in vivo residence time. [ABSTRACT FROM AUTHOR]

Avalanche photodiodes (APDs), and in particular position-sensitive avalanche photodiodes (PSAPDs), are an attractive alternative to photomultiplier tubes (PMTs) for reading out scintillators for PET and SPECT. These solid-state devices offer high gain and quantum efficiency, and can potentially lead to more compact and robust imaging systems with improved spatial and energy resolution. In order to evaluate this performance improvement, we have conducted Monte Carlo simulations of gamma cameras based on avalanche photodiodes. Specifically, we investigated the relative merit of discrete and PSAPDs in a simple continuous crystal gamma camera. The simulated camera was composed of either a 4 × 4 array of four channels 8 × 8 mm2PSAPDs or an 8 × 8 array of 4 × 4 mm2discrete APDs. These configurations, requiring 64 channels readout each, were used to read the scintillation light from a 6 mm thick continuous CsI:Tl crystal covering the entire 3.6 × 3.6 cm2photodiode array. The simulations, conducted with GEANT4, accounted for the optical properties of the materials, the noise characteristics of the photodiodes and the nonlinear charge division in PSAPDs. The performance of the simulated camera was evaluated in terms of spatial resolution, energy resolution and spatial uniformity at 99mTc (140 keV) and 125I (30 keV) energies. Intrinsic spatial resolutions of 1.0 and 0.9 mm were obtained for the APD- and PSAPD-based cameras respectively for 99mTc, and corresponding values of 1.2 and 1.3 mm FWHM for 125I. The simulations yielded maximal energy resolutions of 7% and 23% for 99mTc and 125I, respectively. PSAPDs also provided better spatial uniformity than APDs in the simple system studied. These results suggest that APDs constitute an attractive technology especially suitable to build compact, small field of view gamma cameras dedicated, for example, to small animal or organ imaging. [ABSTRACT FROM AUTHOR]

Electron swarm parameters, such as the drift velocity and the ionization coefficient, in water vapour have been measured for relatively wide ranges in reduced electric fields (E/N) at room temperature. The drift velocity (Wm) was obtained based upon the arrival-time spectra of electrons by using a double-shutter drift tube for the E/Nfrom 60 to 1000?Td, while the first and second ionization coefficients (? and ?) were determined by the steady-state Townsend method from 50 to 3000?Td. A comparison between the results and other data in the literature shows that our results for both the drift velocity and the effective ionization coefficient are lower than those of the other data in the above ranges. [ABSTRACT FROM AUTHOR]

The distribution of calcium ion (Ca-ion) was demonstrated in the mantle epithelial cells by means of two-step chemical precipitation of Ca-ion in the pulmonate snail, Euhadra hickonis. K-oxalate/K-antimonate chemical replacement was done using a simultaneous computerized microwave-stimulated fixation method. These precipitates in the mantle epithelial cells were studied by electron-probe X-ray microanalysis (EDX) and electron energy-filtered imaging (EFI). The calculated values of the essential-elemental X-ray pulse ratios, which were obtained by computerized EDX analysis, agreed with those of the standard samples and theoretical values of Ca-antimonate. Typical EFI images and spectra energy of Ca and Sb were obtained from the surface mantle epithelial cells in the same tissue block as that used for EDX analysis where the elemental distribution of Ca and Sb was the same. The Ca-antimonate precipitates were formed in the intercellular space, vesicles and microvilli of mantle cells. In day 13 embryos, the columella and peripheral area were almost calcified, but in the dorsal area, Ca was still in the ionic state; the binding ratio of the former case was Ca:Sb = 1:0, that of the latter was Ca:Sb = 1:2, and that of the small intermediate area was Ca:Sb = 1:1.4. After day 17, the embryonal periostracum was almost calcified. Between days 15 and 20, strong Ca2+-ATPase and carbonic anhydrase (CAHase) enzymatic activities were observed at the sites where Ca-ion was observed; intercellular spaces, vacuoles in the mantle cells, periostracum and homogeneous layer between the periostracum and mantle cell, and in the mitochondria. Ca2+-ATPase may play important roles in the transportation of Ca-ion from the basal cell layer to the surface layer of the mantle cells and CAHase may play important roles in the chemical binding of Ca-ion and carbonate ion to produce calcium carbonate crystals in the newly developing embryonal shell. [ABSTRACT FROM AUTHOR]

Background/Purpose. Radiofrequency ablation (RFA) and microwave coagulation therapy (MCT) have been gaining acceptance as a standard method in the management strategy of liver cancer, for reasons of minimally invasive techniques and effective results. We present our experience of RFA and MCT in patients with liver cancer, and analyze retrospectively the advantages and disadvantages of both of the percutaneous and laparoscopic approaches. Methods. Thirty-two consecutive patients (23 men and 9 women) with 19 hepatocellular carcinomas (HCC), 12 metastatic liver cancers, and recurrent cholangiocellular carcinoma (CCC), were enrolled in this study. Out of these 32 patients, as a prior laparotomy, 19 underwent hepatectomy, colectomy, gastrectomy or cholecystectomy, and 15 were treated with the laparoscopic approach, 17 treated with the percutaneous approach, and 2 treated with the combined approach of those two. All of these procedures were carried out under general anesthesia with ultrasound guidance. Seven and 30 days after these procedures, an assessment helical computed tomography was done. Results. No sign of the residual tissues was noted in all patients except only one case. Conclusions. The percutaneous approach was thought to be a more practical and less invasive method regardless previous laparotomy. For the laparoscopic approach, tumors located at the hepatic surface or margin were preferable candidates. [ABSTRACT FROM AUTHOR]

Eigen-vortical-axis line proposed as a vortical axis is analysed with respect to the characteristics of the passage of the core region of a vortex in isotropic homogeneous turbulence. Comparison with the vorticity line shows that the difference between them is derived from the geometrical features of a vortex especially vorticity components parallel to swirl plane in weak region of a vortex. Eigen-vortical-axis line tends to pass through the core region with strong intensity of swirling (swirlity) and vorticity, and high flow symmetry. [ABSTRACT FROM AUTHOR]

Energy storage systems are necessary for renewable energy sources such as solar power in order to stabilize their output power, which fluctuates widely depending on the weather. Since ‘flywheel energy storage systems’ (FWSSs) do not use chemical reactions, they do not deteriorate due to charge or discharge. This is an advantage of FWSSs in applications for renewable energy plants. A conventional FWSS has capacity limitation because of the mechanical bearings used to support the flywheel. Therefore, we have designed a superconducting magnetic bearing composed of a superconducting coil stator and a superconducting bulk rotor in order to solve this problem, and have experimentally manufactured a large scale FWSS with a capacity of 100 kWh and an output power of 300 kW. The superconducting magnetic bearing can levitate 4 tons and enables the flywheel to rotate smoothly. A performance confirmation test will be started soon. An overview of the superconducting FWSS is presented in this paper. [ABSTRACT FROM AUTHOR]

The behavior of the nuclear shell structure far away from the valley of stability is discussed in this paper with an emphasis on the N = 20 magic number. Two experimental examples, namely g-spectroscopy of 27Ne by 1H(28Ne, 27Ne) reaction and single-particle spectroscopy of 23O by 22O(d, p)23O* - 22O+n process in inverse kinematics, are shown providing indirect indication and a direct evidence for the extreme decrease of the N = 20 shell gap and, consequently, the vanishing N = 20 magicity. [ABSTRACT FROM AUTHOR]

Introduction: The slow atrioventricular nodal pathway (SP) typically has a single atrial breakthrough site., Methods and Results: We report a case of fast-slow type AVNRT with two distinct atrial breakthrough sites during tachycardia. The earliest atrial activation site (EAAS) was at the right inferior septum, followed by the inferolateral wall of the tricuspid annulus. Ablation at the EAAS shifted it to the inferolateral wall., Conclusion: This case demonstrates two distinct atrial breakthrough sites from a single SP in AVNRT., (© 2024 Wiley Periodicals LLC.)