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In this work we propose two solutions to the problem we consider when we have multivariate experimental data of individuals with structure of groups that have been repeated at different occasions or experimental situations. We obtain the first solution applying dual version of canonical STATIS proposed by Vallejo-Arboleda et al. (2007), and the second with doubly multivariate repeated measures model. We use the data of SWALE project (Stephen et al. 2004), with 7 physical-chemical variables, measured in 4 treatments, each one with 9 repetitions and during 4 weeks (selected between 10 original weeks in the project) to compare these solutions.En este trabajo proponemos dos soluciones al problema que se plantea cuando se pretende analizar datos multivariantes, para un conjunto de individuos con estructura de grupos, que además han sido replicados bien sea en ocasiones o en situaciones experimentales diferentes. La primera solución se obtiene aplicando la versión dual del STATIS canónico propuesto por Vallejo- Arboleda et al. (2007); la segunda, aplicando el modelo de medidas repetidas doblemente multivariantes. Usamos los datos del proyecto SWALE (Stephen et al. 2004) para una selección de 7 variables físico-químicas medidas para 4 tratamientos, cada uno con 9 réplicas, durante 4 semanas (elegidas entre las 10 iniciales del proyecto) para comparar las dos soluciones.

The search for natural food additives makes propolis an exciting alternative due to its known antimicrobial activity. This work aims to investigate propolis' behavior as a nitrite substitute ingredient in cooked ham (a ready-to-eat product) when confronted with pathogenic microorganisms of food interest. The microbial evolution of Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, and Clostridium sporogenes inoculated at known doses was examined in different batches of cooked ham. The design of a challenge test according to their shelf life (45 days), pH values, and water activity allowed the determination of the mesophilic aerobic flora, psychotropic, and acid lactic bacteria viability. The test was completed with an organoleptic analysis of the samples, considering possible alterations in color and texture. The cooked ham formulation containing propolis instead of nitrites limited the potential growth (δ < 0.5 log10) of all the inoculated microorganisms until day 45, except for L. monocytogenes, which in turn exhibited a bacteriostatic effect between day 7 and 30 of the storage time. The sensory analysis revealed the consumer's acceptance of cooked ham batches including propolis as a natural additive. These findings suggest the functionality of propolis as a promising alternative to artificial preservatives for ensuring food safety and reducing the proliferation risk of foodborne pathogens in ready-to-eat products. [ABSTRACT FROM AUTHOR]

Objective: To determine if the addition of the assessment of levator ani muscle (LAM) avulsion to the measurement of the difference in the pubis‐uterine fundus distance between rest and with the Valsalva maneuver could increase the diagnostic capacity of ultrasound for uterine prolapse (UP). Methods: This multicenter, observational and prospective study included 145 patients. Ultrasound assessment was performed, establishing the diagnosis of UP as a difference between the pubic‐uterine fundus distance at rest and during the Valsalva maneuver ≥15 mm (standard technique), while LAM avulsion was defined as an abnormal LAM insertion in three central slices using multislice ultrasound. A binary multivariate logistic regression model was made using nonautomated methods to predict surgical UP (general population, premenopausal, and postmenopausal patients), including the difference between the pubis‐uterine fundus distance at rest and with the Valsalva maneuver as well as LAM avulsion. Results: A total of 143 patients completed the study. The addition of LAM avulsion criteria to the standard dynamic distance‐based protocol for the diagnosis of UP resulted in a higher sensitivity for the general population (79.7 vs 68.1%) as well as for premenopausal (89.3 vs 79.9%) and postmenopausal patients (76 vs 66.1%). In contrast, the standard technique showed a higher specificity than the model based on the standard technique associated with LAM avulsion for the general population (89.2 vs 74.3%) and premenopausal women (91.7 vs 63.2%). For postmenopausal patients, the model based on the standard technique associated with LAM avulsion had a higher sensitivity (76 vs 66.1%) and specificity (91.7 vs 86.8%) than the ultrasound diagnosis of UP. Conclusion: The implementation of the assessment of LAM avulsion in the ultrasound diagnosis of UP is useful in postmenopausal patients, increasing sensitivity and specificity relative to the ultrasound assessment based only on the difference between the pubis‐uterine fundus distance at rest and with the Valsalva maneuver. [ABSTRACT FROM AUTHOR]

Background: Caesarean section is necessary to save the lives of mothers and newborns at times, but it is important to perform it only when it is essential due to all the risks involved. This study aimed to examine the rate of caesarean sections performed at a tertiary hospital using the Robson classification to detect methods for the detection of and/or reduction in these caesarean section rates. Methods: A descriptive, cross-sectional study of a retrospective database was carried out. Results: A total of 10,317 births were assessed. The Robson classification was used to assess these interventions and verify whether the indication for performed caesarean sections was appropriate. In total, 2036 births by caesarean section were performed in the whole sample. The annual caesarean section rate varied between 18.67% and 21.18%. Conclusions: Caesarean sections increased by about 20% in 2021 compared to 2020 even though the trend over the years of study was decreasing. Vaginal delivery after caesarean section is a reasonable and safe option. Caesarean section rates could be improved, mostly in Robson's Group 2. The Robson classification facilitated progress in the implementation of measures aimed at improving care and adjusting caesarean section rates. [ABSTRACT FROM AUTHOR]

The antioxidant, anti-inflammatory, and antimicrobial characteristics of propolis, a bioactive compound collected from hives, have prompted its use in the food sector in recent times. This study investigated the physicochemical characteristics, phenolic profile, and antioxidant capacity of 31 propolis extracts collected from Northern Spain. The physicochemical composition (resins, waxes, ashes mineral content, and heavy metals) was within the allowable regulatory limits. The analysis of bioactive compounds enabled the identification of 51 constituents: flavonoids (apigenin, catechin, chrysin, quercetin, and pinocembrin) and phenolic acids (caffeic, ferulic, and coumaric). The mean value of total polyphenols was 42.72 ± 13.19 Pinocembrin–Galangin Equivalents/100 g, whereas a range between 1.64 ± 0.04 and 4.95 ± 0.36 Quercetin Equivalents (QE) g/100 g was found for total flavonoids content. The determination of bioactivities revealed significant antioxidant capacity using DPPH (1114.28 ± 10.39 µM Trolox Equivalents and 3487.61 ± 318.66 µM Vitamin C Equivalents). Resin content in propolis samples was positively and significantly correlated with both polyphenols (rho = 0.365; p = 0.043) and flavonoid composition (rho = 0.615; p = 0.000) as well as the antioxidant capacity TEAC DPPH (rho = 0.415; p = 0.020). A multiple regression analysis modeled the correlation between resin composition, flavonoids, and TEAC DPPH values, yielding a significant regression equation (R2 = 0.618; F (2,28) = 22.629; p < 0.000; d = 2.299). Therefore, evaluating physicochemical parameters and biological activities provides a promising framework for predicting propolis' quality and antioxidant properties, thus suggesting its potential as a functional and bioactive compound for the food industry. [ABSTRACT FROM AUTHOR]

Objectives: To validate an ultrasound software that uses transperineal ultrasound to diagnose uterine prolapse (UP). Methods: Multicenter, observational and prospective study with 155 patients that had indications for surgical intervention for dysfunctional pelvic floor pathology. Each patient underwent an examination with Pozzi tenaculum forceps was performed in the operating room with the patient anesthetized, followed by surgical correction of stages II–IV UP. Transperineal ultrasound was used to assess the difference in the pubis–uterine fundus measurement. With a multivariate logistic regression binary model (with the measurement ultrasound at rest, the Valsalva maneuver and age) using nonautomated methods to predict UP. With the purpose of evaluating the model, a table with coordinates of the receiver operating characteristic (ROC) curve, after which sensitivity and specificity were assessed. Results: A total of 153 patients were included (73 with a diagnosis of surgical UP). It was obtained from the AUC (0.89) of the probabilities predicted by the model (95% confidence interval, 0.84–0.95; P <.0005). Based on the ROC curve for the model, obtaining a sensitivity of 91.8% and a specificity of 72.7%, values that were superior to those for the clinical exam for surgical UP (sensitivity: 80.8%; specificity: 71.3%). Conclusions: We validated software that uses transperineal ultrasound of the pelvic floor and patient age to generate a more reliable diagnosis of surgical UP than that obtained from clinical examinations. [ABSTRACT FROM AUTHOR]

Objectives: It is unknown whether diagnosing uterine prolapse (UP) via ultrasound or surgical criteria is superior. Our objective is to determine whether the diagnostic capacity of ultrasound with surgical criteria differs from that of surgical criteria only. Methods: This was a multicenter prospective observational study with 54 premenopausal patients with surgical criteria for a dysfunctional pelvic floor pathology who were consecutively recruited for 1 year. Clinical UP with surgical criteria was defined when UP stage II–IV was identified (during pelvic floor consultation), and UP diagnosed by ultrasound with surgical criteria was established when a difference ≥15 mm was found between rest and Valsalva applied to the pubis‐uterine fundus. The sensitivity, specificity and positive and negative predictive values were determined to evaluate clinical and ultrasound methodologies as diagnostic tests. Results: UP diagnosed by ultrasound with surgical criteria presented better sensitivity (78.57 vs 35.71%), specificity (92.11 vs 81.58%), positive predictive value (61.83 vs 23.99%), and negative predictive value (96.35 vs 11.37%) than UP diagnosed by surgical criteria only. Conclusion: Ultrasound with surgical criteria is superior to surgical criteria alone when diagnosing UP. [ABSTRACT FROM AUTHOR]

The prevalence of Listeria monocytogenes in 30 samples of poultry was determined using culture-dependent (isolation on OCLA and confirmation by conventional polymerase chain reaction -PCR-, OCLA&PCR) and culture-independent (real-time polymerase chain reaction, q-PCR) methods. L. monocytogenes was detected in 15 samples (50.0%) by OCLA&PCR and in 20 (66.7%) by q-PCR. The concentrations (log10 cfu/g) of L. monocytogenes (q-PCR) ranged from 2.40 to 5.22 (total cells) and from <2.15 to 3.93 (viable cells). The two methods, q-PCR using a viability marker (v-PCR) and OCLA&PCR (gold standard), were compared for their capacity to detect viable cells of L. monocytogenes, with the potential to cause human disease. The values for sensitivity, specificity and efficiency of the v-PCR were 100%, 66.7% and 83.3%, respectively. The agreement between the two methods (kappa coefficient) was 0.67. The presence of nine virulence genes (hlyA, actA, inlB, inlA, inlC, inlJ, prfA, plcA and iap) was studied in 45 L. monocytogenes isolates (three from each positive sample) using PCR. All the strains harbored between six and nine virulence genes. Fifteen isolates (33.3% of the total) did not show the potential to form biofilm on a polystyrene surface, as determined by a crystal violet assay. The remaining strains were classified as weak (23 isolates, 51.1% of the total), moderate (one isolate, 2.2%) or strong (six isolates, 13.3%) biofilm producers. The strains were tested for susceptibility to a panel of 15 antibiotics. An average of 5.11 ± 1.30 resistances per isolate was observed. When the values for resistance and for reduced susceptibility were taken jointly, this figure rose to 6.91 ± 1.59. There was a prevalence of resistance or reduced susceptibility of more than 50.0% for oxacillin, cefoxitin, cefotaxime, cefepime ciprofloxacin, enrofloxacin and nitrofurantoin. For the remaining antibiotics tested, the corresponding values ranged from 0.0% for chloramphenicol to 48.9% for rifampicin. The high prevalence and level of L. monocytogenes with numerous virulence factors in poultry underline how crucial it is to follow correct hygiene procedures during the processing of this foodstuff in order to reduce the risk of human listeriosis. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the use of intraoperative ultrasound (IUS) to achieve a postoperative optimal placement of the tension‐free vaginal tape‐obturator (TVT‐O). Methods: A cohort study was performed among women who underwent TVT‐O placement. In 25 women, ultrasound was used for the placement, and based on current evidence, the optimal intraoperative and postoperative ultrasound pattern was selected. They were compared with 25 women where IUS was not used. Results: IUS increased operative time (by 7.5 min), and in 36% (9/25) of cases it was necessary to perform some corrections based on the ultrasound findings. After 1 month, in patients of the IUS group, the tapes were more frequently placed at a distance of 3–5 mm (P = 0.01), and it was more common for it to be placed between 40% and 70% relative to the urethral length (P = 0.011). Of tapes placed with IUS, 76% (met the optimal postoperative ultrasound pattern, as opposed to only 48% placed without IUS (P = 0.041). No differences were found in the complications or the functional results at 1 month post‐surgery. Conclusion: The use of IUS for the placement of TVT‐O allows us to position them optimally and avoid erroneous placements, although IUS increases the operative time without improving the functional results and the rate of complications. Synopsis: The use of intraoperative ultrasound for the placement of tension‐free vaginal tape‐obturator improves its optimal positioning and prevents erroneous placements. [ABSTRACT FROM AUTHOR]

Introduction: Forceps use is the main risk factor for levator ani muscle (LAM) injuries. We believe that the disengagement of the forceps branches before delivery of the fetal head could influence LAM injuries, so we aimed to determine the influence of the disengagement of the forceps on the occurrence of LAM avulsion during forceps delivery.Material and Methods: A prospective, observational, multicenter study was conducted with 261 women who underwent forceps delivery. The women were classified according to whether the branches of the forceps had been disengaged before delivery of the fetal head. LAM avulsion was defined using a multislice mode (3 central slices).Results: In all, 255 women completed the study (160 without disengagement and 95 with disengagement). LAM avulsions were observed in 37.9% of women in the group with disengagement and in 41.9% of women in the group without disengagement. The crude OR (without disengagement vs with disengagement) for avulsion was 0.90 (95% CI 0.49-1.67, P = 0.757) and an adjusted OR of 0.82 (95% CI 0.40-1.69, P = 0.603).Conclusions: We did not observe a statistically significant reduction in the LAM avulsion rate with disengagement of the forceps branches before delivery of the fetal head. [ABSTRACT FROM AUTHOR]

Different Machine Learning techniques to detect software vulnerabilities have emerged in scientific and industrial scenarios. Different actors in these scenarios aim to develop algorithms for predicting security threats without requiring human intervention. However, these algorithms require data-driven engines based on the processing of huge amounts of data, known as datasets. This paper introduces the SonarCloud Vulnerable Code Prospector for C (SVCP4C). This tool aims to collect vulnerable source code from open source repositories linked to SonarCloud, an online tool that performs static analysis and tags the potentially vulnerable code. The tool provides a set of tagged files suitable for extracting features and creating training datasets for Machine Learning algorithms. This study presents a descriptive analysis of these files and overviews current status of C vulnerabilities, specifically buffer overflow, in the reviewed public repositories. [ABSTRACT FROM AUTHOR]

Objective: To assess the impact of Triepi-45-a tool that enables an episiotomy angle of 45° to be marked on the perineum at rest-on the incidence of obstetric anal sphincter injuries (OASIS) during operative vaginal delivery (OVD).Method: A retrospective-prospective cohort study was performed among successive women who underwent OVD at Complejo Asistencial Universitario de de León, Spain, between 2011 and 2013 (preintervention cohort, n=986) and between 2014 and 2016 (intervention cohort, n=986) after implementation of an interventional programme in 2013 to improve the episiotomy angle, including use of Triepi-45, in OVD.Results: The intervention cohort had a lower incidence of OASIS than the preintervention cohort (70/986 [7.1%] vs 93/986 [9.4%]), but the difference was not significant, owing to the low use of Triepi-45 in the intervention cohort (n=375). However, the OASIS incidence was significantly lower in the Triepi-45 cohort than in the preintervention cohort (18/375 [4.8%] vs 93/986 [9.4%]; odds ratio, 0.47; 95% confidence interval, 0.26-0.86).Conclusion: Use of Triepi-45 had a positive impact on reducing OASIS in OVD. It remains essential to raise obstetricians' awareness of the importance of the episiotomy angle and to implement the systematic use of tools to reduce the incidence of OASIS. [ABSTRACT FROM AUTHOR]

Beef preparations (meatballs, minced meat, hamburgers, white sausages, and red sausages) from northwest Spain were tested. Microbial counts ranged from 0.70 ± 0.00 log10 cfu/g (enterococci) to 9.57 ± 0.37 log10 cfu/g (psychrotrophs). In 73.3% of cases, total aerobic counts were higher than the microbiological limits set for the end of the manufacturing process in the European Union (EU Regulation 2073/2005). Forty Escherichia coli isolates were tested against thirteen clinically important antibiotics (disk diffusion method; CLSI). Three of the strains (7.5%) were susceptible to all the antibiotics, four (10.0%) showed resistance or reduced susceptibility to one antibiotic, and 33 (82.5%) were multiresistant (with resistance or reduced susceptibility to between two and eight antibiotics), with an average of 1.85 resistances per strain. The highest rates of resistance were observed for two antimicrobials widely used on cattle farms (cefalotin and tetracycline). The findings in this research emphasize the need to correctly handle beef preparations with the aim of reducing risks to consumers. [ABSTRACT FROM AUTHOR]

Introduction: Levator ani avulsion rates after assisted vaginal delivery have been reported in the literature. However, there are no definitive data regarding the association between overdistention and assisted vaginal delivery. Therefore, our aim is to report overdistention rates after assisted vaginal delivery with a postpartum ultrasound examination. Materials and Methods: This multicenter study involved a retrospective analysis of data from primiparous women (n = 602) who had previously been recruited at three tertiary hospitals between January 2015 and January 2017. Overdistention was assessed at 6 months postpartum using three‐/four‐dimensional transperineal ultrasound. Patients with levator ani muscle avulsion were excluded. Overdistention was defined as a levator hiatal area ≥ 25 cm2 on Valsalva. Results: Of the 602 primiparous patients, 250 patients who satisfied the inclusion criteria (139 patients who underwent forceps delivery and 111 patients who underwent vacuum delivery) were evaluated. Overdistention occurred in 20% (50 of 250) of these patients. Overdistention was observed for 1% (1/111) of vacuum deliveries and 35.3% (49 of 139) of forceps deliveries. We found an increased risk of overdistention following forceps delivery compared to vacuum delivery, with a crude odds ratio (OR) of 59.9 (95% confidence interval [CI]: 8.1, 442.2) and an adjusted OR (adjusted for maternal age, second‐stage duration, and head circumference) of 17.6 (95% CI: 2.3, 136.7). Conclusions: Postpartum overdistention occurred for 20% of assisted vaginal deliveries, with an increased risk of overdistention following forceps delivery compared to vacuum delivery. [ABSTRACT FROM AUTHOR]

Objectives: The objective of this study is to investigate a multicenter study to establish if differences exist in the levator ani muscle avulsion (LAM) rates between deliveries performed with Malmstrom's vacuum and the Kiwi vacuum. Study design: A prospective, multicenter observational study with 199 primiparous subjects was performed. All patients had undergone vaginal delivery by vacuum (Malmstrom's or Kiwi). Avulsion was defined as an abnormal insertion of LAM in the lower pubic branch in the multiplanar mode, as identified in the three central sections by transperineal 3/4D echography 6 months after delivery. The area of ​​the levator hiatus was measured in the plane of minimum dimensions at rest, during the Valsalva maneuver and during contraction. Results: LAM avulsion occurred in 33.1% of cases in which Malmstrom's vacuum was used and in 29.4% of cases in which the Kiwi vacuum was used (the difference was not statistically significant), which resulted in a crude odds ratio (OR) of 0.977 (0.426, 2.241; P =.957) and an adjusted OR of 2.90 (0.691; 12.20; P =.146). Women in the Malmstrom's vacuum group had a larger LHA at rest 14.77 vs 12.64 cm2; P =.001) and at maximum contraction (13.41 vs 10.83 cm2; P < 0.001) in comparison with the Kiwi group, although the difference did not reach statistical significance under Valsalva maneuver (18.71 vs 17.21 cm2; P =.051).Differences between both groups were detected in the measurements of the hiatus area levator at rest (14.77 vs 12.64 cm2), during the Valsalva maneuver (18.71 vs 17.21 cm2) and during maximum contraction (13.41 vs 10.83 cm2). Conclusions: In the present study, Malmstrom's vacuum was not associated with a higher risk of LAM in comparison with Kiwi's Omnicup. [ABSTRACT FROM AUTHOR]

Abstract The use of Supercomputers is currently very widespread, constituting an essential component in many fields of science. The interest in the use of high performance computation is increasing in a wider and more diverse population of higher education students, mainly senior undergraduates and postgraduates, because the use of these infrastructures allows learners to improve their skills and the results of their training. For this reason, the demand of courses related to supercomputing increases continuously. In this paper we propose, through a wide review of primary studies, several questions that have been considered as a way of knowing the most widely-used contents in Supercomputing training. We have focused on the factors considered for improving training in Supercomputing, in order to improve the results of researchers in higher education organizations, to identify the limitations of Supercomputing training, and to provide solutions for these limitations. During the search procedure for answering research questions, 1911 studies were considered in the first selection. Through the definition of inclusion and exclusion codes in the results of searching databases, 136 published articles were studied. Finally, using quality criteria, 34 studies were identified as relevant in answering the research questions. Several factors were described, such as the way in which courses related to Supercomputing are organized, the adaptations that are currently being applied in curricula related to the students of these techniques, the use of problem-solving training and the qualification of teachers, among the most relevant ones, as well as several limitations of this type of training and the identification of solutions for these limitations. Data was collected by searching keywords related to Supercomputing training and education in the most important databases used in Computational Science, finding empirical evidence to support the positive effect of High Performance Computers (HPC) on educators and researchers. The implications of this study are: first, it provides a summary of the most relevant factors in improving training, as well as the factors that improve the results through the use of a Supercomputer; and second, it provides the analysis of the limitations found for a better performance of learners and the solutions for these limitations. [ABSTRACT FROM AUTHOR]

Because listeriosis is one of the deadliest foodborne diseases, controlling and eradicating Listeria monocytogenes biofilms is a serious challenge for food safety. Biofilms (24 h old) formed on polystyrene by a L. monocytogenes strain of food origin were exposed for a further 24 h to 12 different concentrations (from 100 to 1011 PFU/mL) of the bacteriophage P100 (Listex P100). The structural parameters of biofilms were studied by using confocal laser scanning microscopy and digital image analysis. The biovolume in the observation field (14,121 μm2) of control (untreated) biofilms was 237,333.1 ± 2,692.6 μm3. The biomass of treated biofilms ranged from 164.7 ± 89.0 μm3 (biofilms exposed to 1010 PFU/mL) to 231,170.5 ± 15,142.0 μm3 (100 PFU/mL). The lowest biomass was achieved after treatment with 108 PFU/mL, with no further decrease in biovolume when higher phage concentrations were used. A strong (P < 0.001) correlation was found between phage concentration (log units) and biovolume (−0.965), surface coverage (−0.939), roughness (0.976), maximum thickness (−0.853), and average thickness (−0.965). Findings from this research suggest that bacteriophage P100 at concentrations equal to or greater than 8 log PFU/mL successfully removes L. monocytogenes biofilms from polystyrene surfaces. [ABSTRACT FROM AUTHOR]

This article assumes the goal of proposing a simulation-based theoretical model comparison methodology with application to two time series road accident models. The model comparison exercise helps to quantify the main differences and similarities between the two models and comprises of three main stages: (1) simulation of time series through a true model with predefined properties; (2) estimation of the alternative model using the simulated data; (3) sensitivity analysis to quantify the effect of changes in the true model parameters on alternative model parameter estimates through analysis of variance, ANOVA. The proposed methodology is applied to two time series road accident models: UCM (unobserved components model) and DRAG (Demand for Road Use, Accidents and their Severity). Assuming that the real data-generating process is the UCM, new datasets approximating the road accident data are generated, and DRAG models are estimated using the simulated data. Since these two methodologies are usually assumed to be equivalent, in a sense that both models accurately capture the true effects of the regressors, we are specifically addressing the modeling of the stochastic trend, through the alternative model. Stochastic trend is the time-varying component and is one of the crucial factors in time series road accident data. Theoretically, it can be easily modeled through UCM, given its modeling properties. However, properly capturing the effect of a non-stationary component such as stochastic trend in a stationary explanatory model such as DRAG is challenging. After obtaining the parameter estimates of the alternative model (DRAG), the estimates of both true and alternative models are compared and the differences are quantified through experimental design and ANOVA techniques. It is observed that the effects of the explanatory variables used in the UCM simulation are only partially captured by the respective DRAG coefficients. This a priori, could be due to multicollinearity but the results of both simulation of UCM data and estimating of DRAG models reveal that there is no significant static correlation among regressors. Moreover, in fact, using ANOVA, it is determined that this regression coefficient estimation bias is caused by the presence of the stochastic trend present in the simulated data. Thus, the results of the methodological development suggest that the stochastic component present in the data should be treated accordingly through a preliminary, exploratory data analysis. [ABSTRACT FROM PUBLISHER]

Virtual reality simulators represent an affordable alternative for teaching and learning specific skills in environments not available in reality for educational purposes. Their goal is to provide a simulated environment where students can practice real tasks in a safer way and with no real consequences in fields as engineering or surgery. For a simulator to become a more complete teaching and learning tool, it should also include the expert knowledge required to provide instant feedback to the user. SHULE is a framework for building haptic simulators to be used witheducational purposes that includes expert knowledge. In order to increase users' motivation, we have also considered game features in the framework design, development and integration with other tools. This process includes the elements needed to provide a challenging goal for the simulator with the correct amount of uncertainty that would increase user's curiosity enough to keep on using it until certain skill is achieved. Simulators produced with SHULE can be integrated with a Learning Management System (LMS) such as Moodle by means of web services. Their functionality offers the teacher the possibility to create new activities to be performed at the simulator, and also offers the students the possibility to see the results of their simulator sessions in the LMS. [ABSTRACT FROM AUTHOR]

The degree of development and operability of the indicators for the Marine Strategy Framework Directive (MSFD) using Descriptor 1 (D1) Biological Diversity was assessed. To this end, an overview of the relevance and degree of operability of the underlying parameters across 20 European countries was compiled by analysing national directives, legislation, regulations, and publicly available reports. Marked differences were found between countries in the degree of ecological relevance as well as in the degree of implementation and operability of the parameters chosen to indicate biological diversity. The best scoring EU countries were France, Germany, Greece and Spain, while the worst scoring countries were Italy and Slovenia. No country achieved maximum scores for the implementation of MSFD D1. The non-EU countries Norway and Turkey score as highly as the top-scoring EU countries. On the positive side, the chosen parameters for D1 indicators were generally identified as being an ecologically relevant reflection of Biological Diversity. On the negative side however, less than half of the chosen parameters are currently operational. It appears that at a pan-European level, no consistent and harmonized approach currently exists for the description and assessment of marine biological diversity. The implementation of the MSFD Descriptor 1 for Europe as a whole can therefore at best be marked as moderately successful. [ABSTRACT FROM PUBLISHER]

Teaching and learning does not only imply transferring knowledge, but also experience. And there is a special kind of experience that involves the use of hand skills and both visual-spatial and bodily-kinesthetic intelligence. One possible solution involves the use of knowledge-based systems to transmit expertise through virtual reality simulators. These simulators have to include the sense of touch, so haptic devices are needed. They have also to act as teaching/learning tools whose goal is to improve students' performance, so individual assessment is a vital characteristic. In order to fulfil these requirements, a framework and the associated methodology for building haptic simulators as learning objects is proposed. The use of this framework reduces the time to develop haptic simulators by using software engineering and including expert knowledge as the core of the system. There are two main actors, besides the user, involved in the development of every new simulator: the expert and the engineer. The expert brings the knowledge to be included in the simulator. The engineer is in charge of building the system using the framework as foundation. The main fields where haptics are being used are engineering and surgery. A proof of concept on surgery is included bringing together engineering and surgery. [ABSTRACT FROM AUTHOR]

The article discusses a study that determines the types of behaviors that occur between humans and dogs to provide insights on how to create robot behaviors. It offers brief overview of the purpose of the study and its proposed concept of social monitoring. However, it argues that the study lacks experimental data regarding the levels of interaction between humans and robot needed for different types of social robots.

The production of H2was studied using a packed bed reactor with polyurethane foam acting as support material. Experiments were performed using mixed microflora under non sterile conditions. The system was initially operated with synthetic wastewater as the sole substrate. Subsequently, cheese whey permeate was added to the system at varying organic loading rates (OLR). The performance of the reactor was evaluated by applying a continuous decrease in OLR. As a result, a significant decrease in H2yields (HY) was observed with the decrease in OLR from 18.8 to 6.3 g chemical oxygen demand (COD)/L d. Microbial analysis demonstrated that the prevalence of non-hydrogen producers,Sporolactobacillus sp.andPrevotella,was the main reason for low HYs obtained. This behavior indicates that the fermentation under non-sterile conditions was favored by high concentrations of substrate by creating an adverse environment for nonhydrogen producer organisms. [ABSTRACT FROM AUTHOR]

The Declaration of Bologna proclaims to change both the academic organization and the way of teaching that are currently implemented in European Higher Education. With regard to teaching methods, an instructional strategy focused on lecturers' teaching is being replaced by methodologies focused on students' learning. However, the application of a student-centered instructional strategy, known as 'active learning', is a formidable task, requiring a great deal of effort from all those involved, plus a suitable instructional process and a set of specific instructional activities. This paper shows the pilot case study of an introductory programming course of the degree in Computer Engineering based on an active learning strategy. Such a pilot case study was carried out to analyze the outcomes of applying the Bologna principles and, based upon those outcomes, to identify the needs and distinctiveness of the Declaration as well as the methodological changes required to implement such principles. During a whole semester, learners followed a continuous assessment process and a student centered instructional strategy that involved an extra effort from both students and teachers. The results show learners benefited from the experience but the system is not entirely prepared to support the new instructional process and several adaptations should be made. Throughout this paper, in addition to the explanation of the pilot study, a set of recommendations, instructional principles, educational tools, and methodological directions will be outlined in order to provide a discussion framework that stimulates and focuses the debate on the application of the Bologna principles to the learning programming activity. [ABSTRACT FROM AUTHOR]

The basic idea of service-oriented learning is that a learning environment should be conceived as a set of independent units of learning packaged as learning services. The design, development and deployment of a learning system based on integrating different learning services needs both a technological platform to support the system as well as a methodology that manages the development process. Such technological platform is provided by the Service-Oriented Learning Architecture (SOLA). SOLA proposes an architecture style, which defines how to deploy several units of packeged-learning services. The purpose of this paper is to review the SOLA concept and to present a specific method based on specifying instructional domains, to complement the SOLA approach. Our method suggests the definition of a feature model, which represents the characteristics of the instructional domain, in order to address the identification and clasiffication of candidate services. The approach here proposed facilitates the definition of candidate-learning services in order to enhance the integration of such services. [ABSTRACT FROM AUTHOR]

Simple Summary: Listeria monocytogenes is the bacterium responsible for the majority of cases of human listeriosis, a foodborne infection that, in certain groups in the population (children, elderly, pregnant women and immunocompromised individuals), exhibits a high fatality rate (of up to 30%), and the need for hospital admission in more than 90% of cases. An awareness of the minimal concentrations for disinfectants and antibiotics necessary to destroy L. monocytogenes, may assist with choosing the most effective antimicrobials for controlling this microorganism, whether in the food industry or in the health system. The lethal concentrations of three disinfectants (sodium hypochlorite, benzalkonium chloride, and peracetic acid) and eight antibiotics (ampicillin, cephalothin, cefoxitin, erythromycin, chloramphenicol, gentamicin, tetracycline, vancomycin, and fosfomycin) for eight different strains of L. monocytogenes were determined in this research work. It was demonstrated that the lethal concentrations for the disinfectants tested were much lower than the concentrations customarily used of these compounds. The characteristics of the cell surface play an important role in the tolerance of L. monocytogenes to these biocides. A considerable prevalence of resistance to most of the antibiotics tested was noted, making it clear that the necessary measures to control resistance in L. monocytogenes must be adopted. When selecting effective doses of antimicrobials, be they biocides or antibiotics, it is essential to know the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of these substances. The present research determined the MICs and MBCs for three biocides, sodium hypochlorite (SH), benzalkonium chloride (BC), and peracetic acid (PAA), and nine antibiotics in eight strains of Listeria monocytogenes of varying serotypes. Marked intra-species differences were observed in the resistance of L. monocytogenes to the biocides and antibiotics. The MICs (ppm) for the biocides ranged between 1750 and 4500 for SH, 0.25 and 20.00 for BC, and 1050 and 1700 for PAA. Their MBCs (ppm) ranged from 2250 to 4500 for SH, 0.50 to 20.00 for BC, and 1150 to 1800 for PAA. The MICs (ppm) for antibiotics lay between 1 and 15 for ampicillin, 8 and 150 for cephalothin, 20 and 170 for cefoxitin, 0.05 and 0.20 for erythromycin, 4 and 50 for chloramphenicol, 3 and 100 for gentamicin, 2 and 15 for tetracycline, 2 and 80 for vancomycin, and 160 and 430 for fosfomycin. The corresponding MBCs (ppm) were from 5 to 20 for ampicillin, 9 to 160 for cephalothin, 70 to 200 for cefoxitin, 4 to 5 for erythromycin, 9 to 70 for chloramphenicol, 5 to 100 for gentamicin, 3 to 30 for tetracycline, 3 to 90 for vancomycin, and 160 to 450 for fosfomycin. Notably, erythromycin showed considerable efficacy, demonstrated by the low values for both MIC and MBC. Based on EUCAST and the CLSI criteria, all strains were susceptible to erythromycin. All strains were resistant to cephalothin, cefoxitin, gentamicin, and fosfomycin. Further values for resistance were 87.50% for ampicillin and vancomycin, 75.00% for tetracycline, and 62.50% for chloramphenicol. The high prevalence of antibiotic resistance is a matter for concern. A positive correlation was found between MIC and MBC values for most of the biocides and antibiotics. The higher the hydrophobicity of the cell surface, the higher the susceptibility to biocides, suggesting that surface characteristics of bacterial cells influence resistance to these compounds. [ABSTRACT FROM AUTHOR]

The development of computer-supported learning systems is a complex task that must take into account diverse issues and perspectives. Given the difficulties attached to this process, different authors have proposed the use of software engineering as a reference to optimize the courseware development process. Following this trend, and given the efficiency of the outcomes, it seems convenient to adapt existing product line development principles from software engineering to the development of learning resources. The purpose of this paper is to postulate a specific analysis method for computer-supported learning systems that guarantees the quality of the development. This specific analysis method can facilitate the learning object reusability and minimize the relevance of the expertise in the development of learning resources. [ABSTRACT FROM AUTHOR]

Instructional engineering provides methods to conduct the design and adaptation of competence development programmes by the combination of diverse learning components (i.e. units of learning, learning activities, learning resources and learning services). It occurs through an established process workflow in which models with diverse levels of abstraction are used to depict such learning components. This paper presents a model-driven generative method used to adapt and reuse a set of learning components for the delivery of a competence development programme concerned with a given learning objective and which serves for a specific instructional context. [ABSTRACT FROM AUTHOR]

In this paper, the authors describe a mechanism for the introduction of small variations in the original learning design process defined in a particular Unit of Learning (UoL). The objective is to increase the UoL reusability by offering the designers an alternative to introduce slight variations on the original design instead of creating a new one each time they want to reuse it. No changes or extensions to the Learning Design definition are required to perform these modifications. The use of design patterns to include the adaptations offers the possibility to easily introduce new operations, such as tracing the activity progress, for instance. The structure of a Learning Design player that is able to process the desired adaptation information and to apply it at runtime will be outlined. The player will be part of an architecture for the automatic adaptation of UoLs to their actual context of execution. [ABSTRACT FROM AUTHOR]

Background: The prognostic value of a prolonged QT interval in SARS-Cov2 infection is not well known. Objective: To determine whether the presence of a prolonged QT on admission is an independent factor for mortality in SARS-Cov2 hospitalized patients. Methods: Single-center cohort of 623 consecutive patients with positive polymerase-chain-reaction test (PCR) to SARS Cov2, recruited from 27 February to 7 April 2020. An electrocardiogram was taken on these patients within the first 48 h after diagnosis and before the administration of any medication with a known effect on QT interval. A prolonged QT interval was defined as a corrected QT (QTc) interval >480 milliseconds. Patients were followed up with until 10 May 2020. Results: Sixty-one patients (9.8%) had prolonged QTc and only 3.2% had a baseline QTc > 500 milliseconds. Patients with prolonged QTc were older, had more comorbidities, and higher levels of immune-inflammatory markers. There were no episodes of ventricular tachycardia or ventricular fibrillation during hospitalization. All-cause death was higher in patients with prolonged QTc (41.0% vs. 8.7%, p < 0.001, multivariable HR 2.68 (1.58–4.55), p < 0.001). Conclusions: Almost 10% of patients with COVID-19 infection have a prolonged QTc interval on admission. A prolonged QTc was independently associated with a higher mortality even after adjustment for age, comorbidities, and treatment with hydroxychloroquine and azithromycin. An electrocardiogram should be included on admission to identify high-risk SARS-CoV-2 patients. [ABSTRACT FROM AUTHOR]