100 results on '"Feo, Salvatore"'
Search Results
2. RIP-Chip analysis supports different roles for AGO2 and GW182 proteins in recruiting and processing microRNA targets
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Perconti, Giovanni, Rubino, Patrizia, Contino, Flavia, Bivona, Serena, Bertolazzi, Giorgio, Tumminello, Michele, Feo, Salvatore, Giallongo, Agata, and Coronnello, Claudia
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- 2019
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3. Social stress under binge-like alcohol withdrawal in adolescence: evidence of cannabidiol effect on maladaptive plasticity in rats.
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Brancato, Anna, Castelli, Valentina, Lavanco, Gianluca, D'Amico, Cesare, Feo, Salvatore, Pizzolanti, Giuseppe, Kuchar, Martin, and Cannizzaro, Carla
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CANNABIDIOL ,ANIMAL behavior ,NEURAL transmission ,NEUROENDOCRINE system ,ADRENOCORTICAL hormones ,ALCOHOL withdrawal delirium ,ANIMAL experimentation ,BASAL ganglia ,BINGE drinking ,NEUROPLASTICITY ,RATS ,DOPAMINE ,MOLECULAR biology ,TEENAGERS' conduct of life ,EXCITATORY amino acid agents ,RESEARCH funding ,PSYCHOLOGICAL stress ,ADOLESCENCE - Abstract
Background: Alcohol binge drinking may compromise the functioning of the nucleus accumbens (NAc), i.e. the neural hub for processing reward and aversive responses. Methods: As socially stressful events pose particular challenges at developmental stages, this research applied the resident–intruder paradigm as a model of social stress, to highlight behavioural neuroendocrine and molecular maladaptive plasticity in rats at withdrawal from binge-like alcohol exposure in adolescence. In search of a rescue agent, cannabidiol (CBD) was selected due to its favourable effects on alcohol- and stress-related harms. Results: Binge-like alcohol exposed intruder rats displayed a compromised defensive behaviour against the resident and a blunted response of the stress system, in addition to indexes of abnormal dopamine (DA)/glutamate plasticity and dysfunctional spine dynamics in the NAc. CBD administration (60 mg/kg) was able to: (1) increase social exploration in the binge-like alcohol exposed intruder rats, at the expenses of freezing time, and in control rats, which received less aggressive attacks from the resident; (2) reduce corticosterone levels independently on alcohol previous exposure; (3) restore DA transmission and (4) facilitate excitatory postsynaptic strength and remodelling. Conclusions: Overall, the maladaptive behavioural and synaptic plasticity promoted by the intersection between binge-like alcohol withdrawal and exposure to adverse social stress can be rescued by a CBD détente effect that results in a successful defensive strategy, supported by a functional endocrine and synaptic plasticity. The current data highlight CBD's relevant therapeutic potential in alcohol- and stress-related harms, and prompt further investigation on its molecular targets. [ABSTRACT FROM AUTHOR]
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- 2023
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4. CBD enhances the cognitive score of adolescent rats prenatally exposed to THC and fine-tunes relevant effectors of hippocampal plasticity.
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Castelli, Valentina, Lavanco, Gianluca, D'Amico, Cesare, Feo, Salvatore, Tringali, Giuseppe, Kuchar, Martin, Cannizzaro, Carla, and Brancato, Anna
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CANNABINOIDS ,CANNABIDIOL ,MAZE tests ,HIPPOCAMPUS (Brain) ,TEENAGERS ,PRENATAL exposure ,SCAFFOLD proteins - Abstract
Introduction: An altered neurodevelopmental trajectory associated with prenatal exposure to ?-9-tetrahydrocannabinol (THC) leads to aberrant cognitive processing through a perturbation in the effectors of hippocampal plasticity in the juvenile offspring. As adolescence presents a unique window of opportunity for "brain reprogramming", we aimed at assessing the role of the nonpsychoactive phytocannabinoid cannabidiol (CBD) as a rescue strategy to temper prenatal THC-induced harm. Methods: To this aim, Wistar rats prenatally exposed to THC (2 mg/kg s.c.) or vehicle (gestational days 5-20) were tested for specific indexes of spatial and configural memory in the reinforcement-motivated Can test and in the aversiondriven Barnes maze test during adolescence. Markers of hippocampal excitatory plasticity and endocannabinoid signaling--NMDAR subunits NR1 and 2A-, mGluR5-, and their respective scaffold proteins PSD95- and Homer 1-; CB1Rand the neuromodulatory protein HINT1 mRNA levels were evaluated. CBD (40 mg/kg i.p.) was administered to the adolescent offspring before the cognitive tasks. Results: The present results show that prenatal THC impairs hippocampal memory functions and the underlying synaptic plasticity; CBD is able to mitigate cognitive impairment in both reinforcement- and aversion-related tasks and the neuroadaptation of hippocampal excitatory synapses and CB1Rrelated signaling. Discussion: While this research shows CBD potential in dampening prenatal THCinduced consequences, we point out the urgency to curb cannabis use during pregnancy in order to avoid detrimental bio-behavioral outcomes in the offspring. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Oxidative stress preconditioning of mouse perivascular myogenic progenitors selects a subpopulation of cells with a distinct survival advantage in vitro and in vivo
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Gargioli, Cesare, Turturici, Giuseppina, Barreca, Maria M., Spinello, Walter, Fuoco, Claudia, Testa, Stefano, Feo, Salvatore, Cannata, Stefano M., Cossu, Giulio, Sconzo, Gabriella, and Geraci, Fabiana
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- 2018
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6. Prenatal Exposure to Δ9-Tetrahydrocannabinol Affects Hippocampus-Related Cognitive Functions in the Adolescent Rat Offspring: Focus on Specific Markers of Neuroplasticity.
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Castelli, Valentina, Lavanco, Gianluca, Feo, Salvatore, D'Amico, Cesare, Micale, Vincenzo, Kuchar, Martin, Plescia, Fulvio, Brancato, Anna, and Cannizzaro, Carla
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COGNITIVE ability ,PRENATAL exposure ,MAZE tests ,OBJECT recognition (Computer vision) ,NEUROPLASTICITY - Abstract
Previous evidence suggests that prenatal exposure to THC (pTHC) derails the neurodevelopmental trajectories towards a vulnerable phenotype for impaired emotional regulation and limbic memory. Here we aimed to investigate pTHC effect on hippocampus-related cognitive functions and markers of neuroplasticity in adolescent male offspring. Wistar rats were exposed to THC (2 mg/kg) from gestational day 5 to 20 and tested for spatial memory, object recognition memory and reversal learning in the reinforce-motivated Can test and in the aversion-driven Barnes maze test; locomotor activity and exploration, anxiety-like behaviour, and response to natural reward were assessed in the open field, elevated plus maze, and sucrose preference tests, respectively. The gene expression levels of NMDA NR1-2A subunits, mGluR5, and their respective scaffold proteins PSD95 and Homer1, as well as CB1R and the neuromodulatory protein HINT1, were measured in the hippocampus. pTHC offspring exhibited deficits in spatial and object recognition memory and reversal learning, increased locomotor activity, increased NR1-, decreased NR2A- and PSD95-, increased mGluR5- and Homer1-, and augmented CB1R- and HINT1-hippocampal mRNA levels. Our data shows that pTHC is associated with specific impairment in spatial cognitive processing and effectors of hippocampal neuroplasticity and suggests novel targets for future pharmacological challenges. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Regulation of Translocated c-myc Genes Transfected into Plasmacytoma Cells
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Feo, Salvatore, Harvey, Richard, Showe, Louise, and Croce, Carlo M.
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- 1986
8. Molecular Cloning and Nucleotide Sequence of a Full-Length cDNA for Human α Enolase
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Giallongo, Agata, Feo, Salvatore, Moore, Robert, Croce, Carlo M., and Showe, Louise C.
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- 1986
9. A Strategy to Detect and Isolate an Intron-Containing Gene in the Presence of Multiple Processed Pseudogenes
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Davies, Brendan, Feo, Salvatore, Heard, Edith, and Fried, Mike
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- 1989
10. Tongue stretching: technique and clinical proposal.
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Buscemi, Andrea, Coco, Marinella, Rapisarda, Alessandro, Frazzetto, Giulia, Di Rosa, Daniela, Feo, Salvatore, Piluso, Marta, Presente, Lilia Paola, Campisi, Santi Scirè, and Desirò, Paolo
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THERAPEUTICS ,SKELETAL muscle ,DEGLUTITION ,POSTURAL balance ,TONGUE ,DEGLUTITION disorders ,TREATMENT effectiveness ,MANIPULATION therapy - Abstract
The tongue is an organ with multiple functions, from sucking to phonation, from swallowing to postural control and equilibrium. An incorrect position or mechanics of the tongue can causes sucking problems in the newborn or atypical swallowing in the adult, with repercussions on the position of the head and neck, up to influencing upright posture and other problems. Tongue dysfunctions are quite frequent (10–15%) in the population. For the manual therapist, this frequency indicates one to two subjects every 30 patients. Exercises have been proposed to improve the tone and strength of the swallowing muscles but the results are not so clear in the literature. The aim of this study is to describe and provide a tongue muscle normalization technique that helps the manual therapist in the treatment of problems related to it. The literature has been investigated through pubmed, Google scholar of the last 10 years, the keywords used and combined with the Boolean operators AND and OR, are: "tongue, tongue habits, tongue diseases, taste disorder, neck pain, posture, postural balance, atypical swallowing, muscle stretching exercise, tissue expansion, soft tissue therapy, osteopathic manipulative treatment". The technique is possible to be executed even in a sitting position, in the case the patient is unable to assume a supine position, the subject should provides immediate feedback that allows the therapist to understand if the technique has been correctly executed. The simplicity of execution and application of the technique makes it a possible and immediate therapeutic tool in the clinical setting. [ABSTRACT FROM AUTHOR]
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- 2022
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11. Chimeric amplicons containing the c-myc gene in HL60 cells
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Mangano, Raffaella, Piddini, Eugenia, Carramusa, Letizia, Duhig, Trevor, Feo, Salvatore, and Fried, Mike
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- 1998
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12. C4, BF, C3 allele distribution and complement activity in healthy aged people and centenarians
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Bellavia, Diego, Frada, Giovanni, Di Franco, Pietro, Feo, Salvatore, Franceschi, Claudio, Sansoni, Paolo, and Brai, Melchiorre
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Antigens -- Research ,Gerontology -- Research ,Aged -- Physiological aspects ,Immune system -- Research ,Health ,Seniors - Abstract
The aim of this study was to examine the complement system and the distribution of some human leukocyte antigen (HLA) class III alleles (C4, BF) in healthy aged people (77 centenarians and 89 elderly subjects). We have also studied the alleles of C3, a complement component genetically unrelated to HLA, the immunochemical levels of C4 and C3 and serum functional hemolytic activity for classical (CH50) and alternative (AP50) complement pathway. The levels of C3 and C4 and the CH50 and AP50 were found to be within the normal range. The frequencies of C3, BF, and C4A alleles were similar in the cohorts that have been studied. For C4B null allele (C4BQ0) a trend toward an increase in the older cohort was observed, although the differences were not significant after statistical correction. Our data suggest that the complement system is well preserved in centenarians and elderly subjects and class III HLA antigens are equally distributed in aged cohorts and in young healthy individuals.
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- 1999
13. Anti-inflammatory effects of annexin-1: stimulation of IL-10 release and inhibition of nitric oxide synthesis
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Ferlazzo, Viviana, DʼAgostino, Pietro, Milano, Salvatore, Caruso, Rosalba, Feo, Salvatore, Cillari, Enrico, and Parente, Luca
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- 2003
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14. Chemically modified tetracyclines induce cytotoxic effects against J774 tumour cell line by activating the apoptotic pathway
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DʼAgostino, Pietro, Ferlazzo, Viviana, Milano, Salvatore, La Rosa, Marzia, Di Bella, Gloria, Caruso, Rosalba, Barbera, Caterina, Grimaudo, Stefania, Tolomeo, Manlio, Feo, Salvatore, and Cillari, Enrico
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- 2003
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15. ENO1 gene product binds to the c- myc promoter and acts as a transcriptional repressor: relationship with Myc promoter-binding protein 1 (MBP-1)
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Feo, Salvatore, Arcuri, Daniela, Piddini, Eugenia, Passantino, Rosa, and Giallongo, Agata
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- 2000
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16. The effects of structural changes on the anti-microbial and anti-proliferative activities of diimidazolium salts.
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Cancemi, Patrizia, Buttacavoli, Miriam, D'Anna, Francesca, Feo, Salvatore, Fontana, Rosa Maria, Noto, Renato, Sutera, Alberto, Vitale, Paola, and Gallo, Giuseppe
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IMIDAZOLES ,ANTI-infective agents ,ERYTHROCYTES - Abstract
An array of diimidazolium salts has been synthesized and used to investigate their anti-microbial and anti-proliferative activities. In particular, salts based on the 3,3′-di-n-alkyl-1,1′-(1,n-phenylenedimethylene)diimidazolium cation and differing in the alkyl chain length on the imidazolium ion, the isomeric substitution on the aromatic spacer and in the anion nature were used. The anti-proliferative activity was evaluated against cervical (HeLa), colon adenocarcinoma (HT-29) and breast (SKBR3) cancer cell lines. In the latter case, also a morphological assessment after treatment with salts was performed. All salts were tested for their hemolytic activity against human erythrocytes. On the other hand, the anti-microbial activity was investigated using both Gram-negative (Escherichia coli) and Gram-positive (Kokuria rhizophila, Staphilococcus aureus and Bacillus subtilis) strains. On the whole, the data collected demonstrate that biological activity is the result of the combined action of both cation and anion structures. In general, the cation hydrophobicity plays the most significant role with structural features of the anion becoming more relevant in the presence of a shorter alkyl chain on the cationic head. [ABSTRACT FROM AUTHOR]
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- 2017
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17. Granulocyte-colony stimulating factor plus plerixafor in patients with β-thalassemia major results in the effective mobilization of primitive CD34+ cells with specific gene expression profile.
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Baiamonte, Elena, Barone, Rita, Contino, Flavia, Di Stefano, Rosalia, Marfia, Anna, Filosa, Aldo, D'Angelo, Emanuela, Feo, Salvatore, Acuto, Santina, and Maggio, Aurelio
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THALASSEMIA treatment ,GRANULOCYTE-macrophage colony-stimulating factor ,GENE therapy ,CD34 antigen ,LEUKAPHERESIS ,GENE expression ,BETA-Thalassemia ,THERAPEUTICS - Abstract
Successful gene therapy for β-thalassemia requires optimal numbers of autologous gene-transduced hematopoietic stem and progenitor cells (HSPCs) with high repopulating capacity. Previous studies suggested superior mobilization in these patients by the combination of granulocyte-colony stimulating factor (G-CSF) plus plerixafor over single agents. We mobilized four adult patients using G-CSF+plerixafor to assess the intra-individual variation of the circulating CD34+ cells number and subtypes pre- and post-plerixafor administration. The procedure was well-tolerated and the target cell dose of ≥8x10
6 CD34+ cells/kg was achieved in three of them with one apheresis procedure. The addition of plerixafor unanimously increased the number of circulating CD34+ cells, and the frequency of the most primitive CD34+ subtypes: CD34+/38- and CD34+/133+/38- as well as the in vitro clonogenic potency. Microarray analyses of CD34+ cells purified from the leukapheresis of one patient mobilized twice, with G-CSF and with G-CSF+plerixafor, highlighted in G-CSF+plerixafor-mobilized CD34+ cells, higher levels of expression genes involved in HSPC motility, homing, and cell cycles. In conclusion, G-CSF+plerixafor in β-thalassemia patients mobilizes optimal numbers of HSPCs with characteristics that suggest high capacity of engraftment after transplantation. [ABSTRACT FROM AUTHOR]- Published
- 2017
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18. The Challenge of Using CB-HSCs As Source for Gene Therapy: Lentiviral Vector Transduction, Phenotypic Characterization and Global Gene Expression Profile of Ex-Vivo Expanded CB CD34+ Cells
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Di Stefano, Rosalia, Baiamonte, Elena, Lo Iacono, Melania, Spina, Barbara, Contino, Flavia, Coronnello, Claudia, Sacco, Massimiliano, Di Maggio, Rosario, Vitrano, Angela, Feo, Salvatore, Maggio, Aurelio, and Acuto, Santina
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- 2015
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19. Hematopoietic Stem Cell Mobilization for Gene Therapy: The Combination of G-CSF+Plerixafor in Patients with Beta-Thalassemia Major Provides High Yields of CD34+ Cells with Primitive Signatures
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Baiamonte, Elena, Barone, Rita, Di Stefano, Rosalia, Lo Iacono, Melania, Spina, Barbara, Contino, Flavia, Di Maggio, Rosario, Sacco, Massimiliano, Vitrano, Angela, Feo, Salvatore, Maggio, Aurelio, and Acuto, Santina
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- 2015
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20. Cellular stress induces cap-independent alpha-enolase/MBP-1 translation.
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Maranto, Cristina, Perconti, Giovanni, Contino, Flavia, Rubino, Patrizia, Feo, Salvatore, and Giallongo, Agata
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ENOLASE ,MYC oncogenes ,CARRIER proteins ,GLYCOLYSIS ,TUMOR suppressor genes ,CELLULAR signal transduction ,GENETIC overexpression - Abstract
Myc promoter-binding protein-1 (MBP-1) is a shorter protein variant of the glycolytic enzyme alpha-enolase. Although several lines of evidence indicate that MBP-1 acts as a tumor suppressor, the cellular mechanisms and signaling pathways underlying MBP-1 expression still remain largely elusive. To dissect these pathways, we used the SkBr3 breast cancer cell line and non-tumorigenic HEK293T cells ectopically overexpressing alpha-enolase/MBP-1. Here, we demonstrate that induced cell stresses promote MBP-1 expression through the AKT/PERK/eIF2α signaling axis. Our results contribute to shedding light on the molecular mechanisms underlying MBP-1 expression in non-tumorigenic and cancer cells. [ABSTRACT FROM AUTHOR]
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- 2015
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21. Vaccination With ENO1 DNA Prolongs Survival of Genetically Engineered Mice With Pancreatic Cancer.
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Cappello, Paola, Rolla, Simona, Chiarle, Roberto, Principe, Moitza, Cavallo, Federica, Perconti, Giovanni, Feo, Salvatore, Giovarelli, Mirella, and Novelli, Francesco
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DNA vaccines ,PANCREATIC cancer treatment ,LABORATORY mice ,PANCREATICODUODENECTOMY ,IMMUNOGLOBULIN G ,PANCREATIC duct ,TUMOR necrosis factors ,MONOCLONAL antibodies ,TUMORS - Abstract
Background & Aims: Pancreatic ductal adenocarcinoma (PDA) is an aggressive tumor, and patients typically present with late-stage disease; rates of 5-year survival after pancreaticoduodenectomy are low. Antibodies against α-enolase (ENO1), a glycolytic enzyme, are detected in more than 60% of patients with PDA, and ENO1-specific T cells inhibit the growth of human pancreatic xenograft tumors in mice. We investigated whether an ENO1 DNA vaccine elicits antitumor immune responses and prolongs survival of mice that spontaneously develop autochthonous, lethal pancreatic carcinomas. Methods: We injected and electroporated a plasmid encoding ENO1 (or a control plasmid) into Kras
G12D /Cre (KC) mice and KrasG12D /Trp53R172H /Cre (KPC) mice at 4 weeks of age (when pancreatic intraepithelial lesions are histologically evident). Antitumor humoral and cellular responses were analyzed by histology, immunohistochemistry, enzyme-linked immunosorbent assays, flow cytometry, and enzyme-linked immunosorbent spot and cytotoxicity assays. Survival was analyzed by Kaplan–Meier analysis. Results: The ENO1 vaccine induced antibody and a cellular response and increased survival times by a median of 138 days in KC mice and 42 days in KPC mice compared with mice given the control vector. On histologic analysis, the vaccine appeared to slow tumor progression. The vaccinated mice had increased serum levels of anti-ENO1 immunoglobulin G, which bound the surface of carcinoma cells and induced complement-dependent cytotoxicity. ENO1 vaccination reduced numbers of myeloid-derived suppressor cells and T-regulatory cells and increased T-helper 1 and 17 responses. Conclusions: In a genetic model of pancreatic carcinoma, vaccination with ENO1 DNA elicits humoral and cellular immune responses against tumors, delays tumor progression, and significantly extends survival. This vaccination strategy might be developed as a neoadjuvant therapy for patients with PDA. [Copyright &y& Elsevier]- Published
- 2013
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22. Negative transcriptional control of ERBB2 gene by MBP-1 and HDAC1: diagnostic implications in breast cancer.
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Contino, Flavia, Mazzarella, Claudia, Ferro, Arianna, Lo Presti, Mariavera, Roz, Elena, Lupo, Carmelo, Perconti, Giovanni, Giallongo, Agata, and Feo, Salvatore
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BREAST cancer prognosis ,GENE expression ,CARRIER proteins ,MYC oncogenes ,GENE targeting ,IMMUNOHISTOCHEMISTRY - Abstract
Background: The human ERBB2 gene is frequently amplified in breast tumors, and its high expression is associated with poor prognosis. We previously reported a significant inverse correlation between Myc promoter-binding protein-1 (MBP-1) and ERBB2 expression in primary breast invasive ductal carcinoma (IDC). MBP-1 is a transcriptional repressor of the c-MYC gene that acts by binding to the P2 promoter; only one other direct target of MBP-1, the COX2 gene, has been identified so far. Methods: To gain new insights into the functional relationship linking MBP-1 and ERBB2 in breast cancer, we have investigated the effects of MBP-1 expression on endogenous ERBB2 transcript and protein levels, as well as on transcription promoter activity, by transient-transfection of SKBr3 cells. Reporter gene and chromatin immunoprecipitation assays were used to dissect the ERBB2 promoter and identify functional MBP-1 target sequences. We also investigated the relative expression of MBP-1 and HDAC1 in IDC and normal breast tissues by immunoblot analysis and immunohistochemistry. Results: Transfection experiments and chromatin immunoprecipitation assays in SKBr3 cells indicated that MBP-1 negatively regulates the ERBB2 gene by binding to a genomic region between nucleotide -514 and -262 of the proximal promoter; consistent with this, a concomitant recruitment of HDAC1 and loss of acetylated histone H4 was observed. In addition, we found high expression of MBP-1 and HDAC1 in normal tissues and a statistically significant inverse correlation with ErbB2 expression in the paired tumor samples. Conclusions: Altogether, our in vitro and in vivo data indicate that the ERBB2 gene is a novel MBP-1 target, and immunohistochemistry analysis of primary tumors suggests that the concomitant high expression of MBP-1 and HDAC1 may be considered a diagnostic marker of cancer progression for breast IDC. [ABSTRACT FROM AUTHOR]
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- 2013
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23. Myc Promoter-Binding Protein-1 (MBP-1) Is a Novel Potential Prognostic Marker in Invasive Ductal Breast Carcinoma.
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Lo Presti, Mariavera, Ferro, Arianna, Contino, Flavia, Mazzarella, Claudia, Sbacchi, Silvia, Roz, Elena, Lupo, Carmelo, Perconti, Giovanni, Giallongo, Agata, Migliorini, Paola, Marrazzo, Antonio, and Feo, Salvatore
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BREAST cancer ,CARRIER proteins ,CYSTS (Pathology) ,ONCOLOGY ,IMMUNOGLOBULINS ,CRYOBIOLOGY ,GENE expression - Abstract
Background: Alphα-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. a-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational starting sites. MBP-1 binds to the P2 c-myc promoter and competes with TATAbox binding protein (TBP) to suppress gene transcription. Although several studies have shown an antiproliferative effect of MBP-1 overexpression on several human cancer cells, to date detailed observations of α-enolase and MBP-1 relative expression in primary tumors versus normal tissues and their correlation with clinicopathological features have not been undertaken. Methodology and Findings: We analyzed α-enolase and MBP-1 expression in normal breast epithelium and primary invasive ductal breast carcinoma (IDC) from 177 patients by Western blot and immunohistochemical analyses, using highly specific anti-α-enolase monoclonal antibodies. A significant increase in the expression of cytoplasmic α-enolase was observed in 98% of the tumors analysed, compared to normal tissues. Nuclear MBP-1 was found in almost all the normal tissues while its expression was retained in only 35% of the tumors. Statistically significant associations were observed among the nuclear expression of MBP-1 and ErbB2 status, Ki-67 expression, node status and tumor grade. Furthermore MBP-1 expression was associated with good survival of patients with IDC. Conclusions: MBP-1 functions in repressing c-myc gene expression and the results presented indicate that the loss of nuclear MBP-1 expression in a large number of IDC may be a critical step in the development and progression of breast cancer and a predictor of adverse outcome. Nuclear MBP-1 appears to be a novel and valuable histochemical marker with potential prognostic value in breast cancer. [ABSTRACT FROM AUTHOR]
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- 2010
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24. Integrated Multi-Omics Investigations of Metalloproteinases in Colon Cancer: Focus on MMP2 and MMP9.
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Buttacavoli, Miriam, Di Cara, Gianluca, Roz, Elena, Pucci-Minafra, Ida, Feo, Salvatore, and Cancemi, Patrizia
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COLON cancer ,PROTEOLYTIC enzymes ,COLORECTAL cancer ,PROGNOSIS ,EPITHELIAL-mesenchymal transition - Abstract
Colorectal cancer (CRC) develops by genetic and epigenetic alterations. However, the molecular mechanisms underlying metastatic dissemination remain unclear and could benefit from multi-omics investigations of specific protein families. Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in ECM remodeling and the processing of bioactive molecules. Increased MMP expression promotes the hallmarks of tumor progression, including angiogenesis, invasion, and metastasis, and is correlated with a shortened survival. Nevertheless, the collective role and the possible coordination of MMP members in CRC are poorly investigated. Here, we performed a multi-omics analysis of MMP expression in CRC using data mining and experimental investigations. Several databases were used to deeply mine different expressions between tumor and normal tissues, the genetic and epigenetic alterations, the prognostic value as well as the interrelationships with tumor immune-infiltrating cells (TIICs). A special focus was placed on to MMP2 and MMP9: their expression was correlated with immune markers and the interaction network of co-expressed genes disclosed their implication in epithelial to mesenchymal transition (EMT) and immune response. Finally, the activity levels of MMP2 and MMP9 in a cohort of colon cancer samples, including tissues and the corresponding sera, was also investigated by zymography. Our findings suggested that MMPs could have a high potency, as they are targeted in colon cancer, and might serve as novel biomarkers, especially for their involvement in the immune response. However, further studies are needed to explore the detailed biological functions and molecular mechanisms of MMPs in CRC, also in consideration of their expression and different regulation in several tissues. [ABSTRACT FROM AUTHOR]
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- 2021
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25. Decorin Transfection Induces Proteomic and Phenotypic Modulation in Breast Cancer Cells 8701-BC.
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Pucci-Minafra, Ida, Cancemi, Patrizia, Cara, Gianluca Di, Minafra, Luigi, Feo, Salvatore, Forlino, Antonella, Tira, M. Enrica, Tenni, Ruggero, Martini, Désirée, Ruggeri, Alessandro, and Minafra, Salvatore
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BREAST cancer ,PROTEOMICS ,CANCER cells ,PROTEOGLYCANS ,EXTRACELLULAR matrix ,CELL proliferation ,CELL adhesion - Abstract
Decorin is a prototype member of the small leucine-rich proteoglycan family widely distributed in the extracellular matrices of many connective tissues, where it has been shown to play multiple important roles in the matrix assembly process, as well as in some cellular activities. A major interest for decorin function concerns its role in tumorigenesis, as growth-inhibitor of different neoplastic cells, and potential antimetastatic agent. The aim of our research was to investigate wide-ranged effects of transgenic decorin on breast cancer cells. To this purpose we utilized the well-characterized 8701-BC cell line, isolated from a ductal infiltrating carcinoma of the breast, and two derived decorin-transfected clones, respectively, synthesizing full decorin proteoglycan or its protein core. The responses to the ectopic decorin production were examined by studying morphological changes, cell proliferation rates, and proteome modulation. The results revealed new important antioncogenic potentialities, likely exerted by decorin through a variety of distinct biochemical pathways. Major effects included the downregulation of several potential breast cancer biomarkers, the reduction of membrane ruffling, and the increase of cell-cell adhesiveness. These results disclose original aspects related to the reversion of malignant traits of a prototype of breast cancer cells induced by decorin. They also raise additional interest for the postulated clinical application of decorin. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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26. The PVT-1 oncogene is a Myc protein target that is overexpressed in transformed cells.
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Carramusa, Letizia, Contino, Flavia, Ferro, Arianna, Minafra, Luigi, Perconti, Giovanni, Giallongo, Agata, and Feo, Salvatore
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NERVOUS system tumors ,MYC oncogenes ,TUMOR proteins ,LYMPHOPROLIFERATIVE disorders ,GENETIC transformation ,PRESERVATION of organs, tissues, etc. - Abstract
The human PVT-1 gene is located on chromosome 8 telomeric to the c-Myc gene and it is frequently involved in the translocations occurring in variant Burkitt's lymphomas and murine plasmacytomas. It has been proposed that PVT-1 regulates c-Myc gene transcription over a long distance. To get new insights into the functional relationships between the two genes, we have investigated PVT-1 and c-Myc expression in normal human tissues and in transformed cells. Our findings indicate that PVT-1 expression is restricted to a relative low number of normal tissues compared to the wide distribution of c-Myc mRNA, whereas the gene is highly expressed in many transformed cell types including neuroblastoma cells that do not express c-Myc. Reporter gene assays were used to dissect the PVT-1 promoter and to identify the region responsible for the elevated expression observed in transformed cells. This region contains two putative binding sites for Myc proteins. The results of transfection experiments in RAT1-MycER cells and chromatin immunoprecipitation (ChIP) assays in proliferating and differentiated neuroblastoma cells indicate that PVT-1 is a downstream target of Myc proteins. J. Cell. Physiol. 213: 511–518, 2007. © 2007 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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27. Binge-like Alcohol Exposure in Adolescence: Behavioural, Neuroendocrine and Molecular Evidence of Abnormal Neuroplasticity... and Return.
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Brancato, Anna, Castelli, Valentina, Lavanco, Gianluca, Tringali, Giuseppe, Micale, Vincenzo, Kuchar, Martin, D'Amico, Cesare, Pizzolanti, Giuseppe, Feo, Salvatore, and Cannizzaro, Carla
- Subjects
CORTICOTROPIN releasing hormone ,REWARD (Psychology) ,ADOLESCENCE ,TEENAGERS ,SYNTHETIC marijuana ,DOPAMINE ,ALCOHOL drinking - Abstract
Binge alcohol consumption among adolescents affects the developing neural networks underpinning reward and stress processing in the nucleus accumbens (NAc). This study explores in rats the long-lasting effects of early intermittent exposure to intoxicating alcohol levels at adolescence, on: (1) the response to natural positive stimuli and inescapable stress; (2) stress-axis functionality; and (3) dopaminergic and glutamatergic neuroadaptation in the NAc. We also assess the potential effects of the non-intoxicating phytocannabinoid cannabidiol, to counteract (or reverse) the development of detrimental consequences of binge-like alcohol exposure. Our results show that adolescent binge-like alcohol exposure alters the sensitivity to positive stimuli, exerts social and novelty-triggered anxiety-like behaviour, and passive stress-coping during early and prolonged withdrawal. In addition, serum corticosterone and hypothalamic and NAc corticotropin-releasing hormone levels progressively increase during withdrawal. Besides, NAc tyrosine hydroxylase levels increase at late withdrawal, while the expression of dopamine transporter, D1 and D2 receptors is dynamically altered during binge and withdrawal. Furthermore, the expression of markers of excitatory postsynaptic signaling—PSD95; Homer-1 and -2 and the activity-regulated spine-morphing proteins Arc, LIM Kinase 1 and FOXP1—increase at late withdrawal. Notably, subchronic cannabidiol, during withdrawal, attenuates social- and novelty-induced aversion and passive stress-coping and rectifies the hyper-responsive stress axis and NAc dopamine and glutamate-related neuroplasticity. Overall, the exposure to binge-like alcohol levels in adolescent rats makes the NAc, during withdrawal, a locus minoris resistentiae as a result of perturbations in neuroplasticity and in stress-axis homeostasis. Cannabidiol holds a promising potential for increasing behavioural, neuroendocrine and molecular resilience against binge-like alcohol harmful effects. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
28. Transcriptomic Changes Following Partial Depletion of CENP-E in Normal Human Fibroblasts.
- Author
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Cilluffo, Danilo, Chiavetta, Roberta Flavia, Bivona, Serena, Contino, Flavia, Coronnello, Claudia, Feo, Salvatore, Di Leonardo, Aldo, and Barra, Viviana
- Subjects
CHROMOSOME segregation ,CHROMOSOME structure ,TRANSCRIPTOMES ,CELL cycle regulation ,GENE expression profiling ,CHROMATIDS - Abstract
The centromere is a fundamental chromosome structure in which the macro-molecular kinetochore assembles and is bound by spindle microtubules, allowing the segregation of sister chromatids during mitosis. Any alterations in kinetochore assembly or functioning or kinetochore–microtubule attachments jeopardize chromosome stability, leading to aneuploidy, a common feature of cancer cells. The spindle assembly checkpoint (SAC) supervises this process, ensuring a faithful segregation of chromosomes. CENP-E is both a protein of the kinetochore and a crucial component of the SAC required for kinetochore–microtubule capture and stable attachment, as well as congression of chromosomes to the metaphase plate. As the function of CENP-E is restricted to mitosis, its haploinsufficiency has been used to study the induced cell aneuploidy; however, the gene expression profile triggered by CENP-E reduction in normal cells has never been explored. To fill this gap, here we investigated whether a gene network exists that is associated with an siRNA-induced 50% reduction in CENP-E and consequent aneuploidy. Gene expression microarray analyses were performed at early and late timepoints after transfection. Initially, cell cycle regulation and stress response pathways were downregulated, while afterwards pathways involved in epithelial–mesenchymal transition, hypoxia and xenobiotic metabolism were altered. Collectively, our results suggest that CENP-E reduction triggers a gene expression program that recapitulates some features of tumor cells. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
29. The human genome contains a single processed pseudogene for a enolase located on chromosome 1.
- Author
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Feo, Salvatore, Oliva, Daniele, Arico, Beatrice, Barba, Giovanna, Cali, Larissa, and Giallongo, Agata
- Published
- 1990
- Full Text
- View/download PDF
30. Conserved alternative splicing in the 5'-untranslated region of the muscle-specific enolase gene.
- Author
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Oliva, Daniele, Venturella, Silvana, Passantino, Rosa, Feo, Salvatore, and Giallongo, Agata
- Subjects
DNA ,MESSENGER RNA ,ENOLASE ,GENETIC engineering ,REVERSE transcriptase ,POLYMERASE chain reaction - Abstract
Reports on the isolation and characterization of DNA covering the region of mouse and rat mRNA that encode the beta or muscle-specific isoform of the glycolytic enzyme enolase. Classes of beta-enolase transcripts with distinct sequences in their regions; Mechanism of alternative splicing; Relative expression of spliced forms in developing and adult muscle tissues by means of reverse transcription and polymerase chain reaction.
- Published
- 1995
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31. Structural features of the human gene for muscle-specific enolase.
- Author
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Giallongo, Agata, Venturella, Silvana, Oliva, Daniele, Barbieri, Giovanna, Rubino, Patrizia, and Feo, Salvatore
- Subjects
ENOLASE ,LYASES ,GLYCOLYSIS ,ENZYMES ,GENES ,MOLECULAR genetics - Abstract
We report here the isolation and characterization of the human gene for the β of muscle-specific isoform of the glycolytic enzyme enolase.The nucleotide sequence analysis revealed structural features, such as organizations as 11 coding exons, the first exon consisting of an untranslated sequence and hence resembling sequences of the other two members of the gene family, the α and γ enolase genes.The β enolase locus spans about 6 kbp genomic DNA.Sequences matching the consensus sequence for muscle-specific regulatory factors are present in the 5'-flanking region and within the first intron.A combination of primer extension, S1 nuclease protection and RNA-sequencing experiments indicates that the gene has a unique transcriptional start site, 26 bp downstream of a TATA-like box; the differential usage of two donor sites within the untranslated exon 1 generates two alternatively spliced transcripts.The existence of the two mRNA, differing from one another in the presence or absence of a 42-nucleotide fragment in the leader sequence, was confirmed by cloning the corresponding cDNA using the rapid amplification of cDNA ends strategy.Secondary-structure predictions indicated that the leader sequences of the spliced forms could form hairpin structures with different free energies of formation, suggesting translational control. [ABSTRACT FROM AUTHOR]
- Published
- 1993
- Full Text
- View/download PDF
32. Structure of the human gene for α-enolase.
- Author
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Giallongo, Agata, Oliva, Daniele, Calì, Larissa, Barba, Giovanna, Barbieri, Giovanna, and Feo, Salvatore
- Subjects
GENES ,ENOLASE ,ENZYMES ,GENOMES ,HAPLOIDY ,EXONS (Genetics) ,BIOCHEMISTRY - Abstract
In mammals there are at least three isoforms of the glycolytic enzyme enolase encoded by three similar genes: α β and γ. In this report we describe the isolation and characterization of the human α-enotase locus. The gene appears to exist as a single copy in the haploid genuine and is composed of 12 exons distributed over more than 18000 bases. The structure of this gene has a high degree of similarity to that of the human and rat γ-enolase genes, with identical positions for all the intron regions. Primer extension and S1 nuclease protection experiments indicate that transcription is initiated at multiple sites. The putative promoter region, like that of other housekeeping genes, lacks canonical TATA and CAAT boxes, is extremely G + C-rich and contains several potential SP1 binding sites. Furthermore, various sequences similar to known regulatory elements were detected. [ABSTRACT FROM AUTHOR]
- Published
- 1990
- Full Text
- View/download PDF
33. Prognostic and Functional Significant of Heat Shock Proteins (HSPs) in Breast Cancer Unveiled by Multi-Omics Approaches.
- Author
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Buttacavoli, Miriam, Di Cara, Gianluca, D'Amico, Cesare, Geraci, Fabiana, Pucci-Minafra, Ida, Feo, Salvatore, Cancemi, Patrizia, and Rosa, Massimo La
- Subjects
BREAST cancer prognosis ,HEAT shock proteins ,BREAST cancer ,GENETIC regulation ,SOOT ,PROGNOSIS ,EPITHELIAL-mesenchymal transition ,BIOLOGICAL networks - Abstract
Simple Summary: In this study, we investigated the expression pattern and prognostic significance of the heat shock proteins (HSPs) family members in breast cancer (BC) by using several bioinformatics tools and proteomics investigations. Our results demonstrated that, collectively, HSPs were deregulated in BC, acting as both oncogene and onco-suppressor genes. In particular, two different HSP-clusters were significantly associated with a poor or good prognosis. Interestingly, the HSPs deregulation impacted gene expression and miRNAs regulation that, in turn, affected important biological pathways involved in cell cycle, DNA replication, and receptors-mediated signaling. Finally, the proteomic identification of several HSPs members and isoforms revealed much more complexity of HSPs roles in BC and showed that their expression is quite variable among patients. In conclusion, we elaborated two panels of HSPs that could be further explored as potential biomarkers for BC progression and prognosis. Heat shock proteins (HSPs) are a well-characterized molecular chaperones protein family, classified into six major families, according to their molecular size. A wide range of tumors have been shown to express atypical levels of one or more HSPs, suggesting that they could be used as biomarkers. However, the collective role and the possible coordination of HSP members, as well as the prognostic significance and the functional implications of their deregulated expression in breast cancer (BC) are poorly investigated. Here, we used a systematic multi-omics approach to assess the HSPs expression, the prognostic value, and the underlying mechanisms of tumorigenesis in BC. By using data mining, we showed that several HSPs were deregulated in BC and significantly correlated with a poor or good prognosis. Functional network analysis of HSPs co-expressed genes and miRNAs highlighted their regulatory effects on several biological pathways involved in cancer progression. In particular, these pathways concerned cell cycle and DNA replication for the HSPs co-expressed genes, and miRNAs up-regulated in poor prognosis and Epithelial to Mesenchymal Transition (ETM), as well as receptors-mediated signaling for the HSPs co-expressed genes up-regulated in good prognosis. Furthermore, the proteomic expression of HSPs in a large sample-set of breast cancer tissues revealed much more complexity in their roles in BC and showed that their expression is quite variable among patients and confined into different cellular compartments. In conclusion, integrative analysis of multi-omics data revealed the distinct impact of several HSPs members in BC progression and indicate that collectively they could be useful as biomarkers and therapeutic targets for BC management. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
34. Proteomic Profiling of Colon Cancer Tissues: Discovery of New Candidate Biomarkers.
- Author
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Buttacavoli, Miriam, Albanese, Nadia Ninfa, Roz, Elena, Pucci-Minafra, Ida, Feo, Salvatore, and Cancemi, Patrizia
- Subjects
COLON cancer ,PROTEOMICS ,BIOMARKERS ,CELLULAR signal transduction ,TISSUES ,APOPTOSIS ,CELL motility - Abstract
Colon cancer is an aggressive tumor form with a poor prognosis. This study reports a comparative proteomic analysis performed by using two-dimensional differential in-gel electrophoresis (2D-DIGE) between 26 pooled colon cancer surgical tissues and adjacent non-tumoral tissues, to identify potential target proteins correlated with carcinogenesis. The DAVID functional classification tool revealed that most of the differentially regulated proteins, acting both intracellularly and extracellularly, concur across multiple cancer steps. The identified protein classes include proteins involved in cell proliferation, apoptosis, metabolic pathways, oxidative stress, cell motility, Ras signal transduction, and cytoskeleton. Interestingly, networks and pathways analysis showed that the identified proteins could be biologically inter-connected to the tumor-host microenvironment, including innate immune response, platelet and neutrophil degranulation, and hemostasis. Finally, transgelin (TAGL), here identified for the first time with four different protein species, collectively down-regulated in colon cancer tissues, emerged as a top-ranked biomarker for colorectal cancer (CRC). In conclusion, our findings revealed a different proteomic profiling in colon cancer tissues characterized by the deregulation of specific pathways involved in hallmarks of cancer. All of these proteins may represent promising novel colon cancer biomarkers and potential therapeutic targets, if validated in larger cohorts of patients. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
35. Anti-Inflammatory Action of Heterogeneous Nuclear Ribonucleoprotein A2/B1 in Patients with Autoimmune Endocrine Disorders.
- Author
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Coppola, Antonina, Cancemi, Patrizia, Tomasello, Laura, Guarnotta, Valentina, Pitrone, Maria, Failla, Valentina, Cillino, Salvatore, Feo, Salvatore, Pizzolanti, Giuseppe, and Giordano, Carla
- Subjects
HUMAN stem cells ,ENDOCRINE diseases ,BLOOD cells ,AUTOIMMUNE diseases ,PROTEIN expression - Abstract
Our previous studies documented that human fibroblast-limbal stem cells (f-LSCs) possess immunosuppressive capabilities, playing a role in regulating T-cell activity. This study highlights the molecular activities by which human f-LSCs can attenuate the inflammatory responses of self-reactive peripheral blood mononuclear cells (PBMCs) collected from patients with autoimmune endocrine diseases (AEDs). Anti-CD3 activated PBMCs from twenty healthy donors and fifty-two patients with AEDs were cocultured on f-LSC monolayer. 2D-DIGE proteomic experiments, mass spectrometry sequencing and functional in vitro assays were assessed in cocultured PBMCs. We identified the downmodulation of several human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) isoforms in healthy and AED activated PBMCs upon f-LSC interaction. The reduction of hnRNPA2/B1 protein expression largely affected the cycling ki67
+ , CD25+ , PD-1+ reactive cells and the double marked CD8+ /hnRNPA2B1+ T cell subset. Anti-PD1 blocking experiments evoked hnRNPA2/B1 overexpression, attributing putative activation function to the protein. hnRNPA2/B2 transient silencing inverted immunopolarization of the self-reactive PBMCs from AEDs toward a M2/Th2-type background. Pharmacological inhibition and co-immunoprecipitation experiments demonstrated the involvement of NF-ĸB in hnRNPA2/B activity and turnover. Our data indicate cardinal involvement of hnRNP A2/B1 protein in peripheral mechanisms of tolerance restoration and attenuation of inflammation, identifying a novel immunoplayer potentially targetable in all AEDs. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
36. Suppression of the normal mouse c-myc oncogene in human lymphoma cells.
- Author
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Feo, Salvatore, ar-Rushdi, Abbas, Huebner, Kay, Finan, Janet, Nowell, Peter C., Clarkson, Bayard, and Croce, Carlo M.
- Published
- 1985
- Full Text
- View/download PDF
37. Kinetics of Labeling of the «Cap» of the Nuclear and Cytoplasmic RNA in Sea-urchin Embryos.
- Author
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Sconzo, Gabriella, di Bernardo, Maria Grazia, di Carlo, Marta, di Liberto, Maurizio, Faraci, Maria Teresa, Feo, Salvatore, Giallongo, Agata, Roccheri, Maria Carmela, and Giudice, Giovanni
- Published
- 1978
- Full Text
- View/download PDF
38. Expression of Alpha-Enolase (ENO1), Myc Promoter-Binding Protein-1 (MBP-1) and Matrix Metalloproteinases (MMP-2 and MMP-9) Reflect the Nature and Aggressiveness of Breast Tumors.
- Author
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Cancemi, Patrizia, Buttacavoli, Miriam, Roz, Elena, and Feo, Salvatore
- Subjects
MATRIX metalloproteinases ,BREAST tumors ,BREAST cancer ,EXTRACELLULAR matrix ,NATURE - Abstract
Breast cancer is a complex and heterogeneous disease: Several molecular alterations cause cell proliferation and the acquisition of an invasive phenotype. Extracellular matrix (ECM) is considered essential for sustaining tumor growth and matrix metalloproteinases (MMPs) have been identified as drivers of many aspects of the tumor phenotype. Mounting evidence indicates that both α-enolase (ENO1) and Myc promoter-binding protein-1 (MBP-1) also played pivotal roles in tumorigenesis, although as antagonists. ENO1 is involved in cell growth, hypoxia tolerance and autoimmune activities besides its major role in the glycolysis pathway. On the contrary, MBP-1, an alternative product of ENO1, suppresses cell proliferation and the invasive ability of cancer cells. Since an important task in personalized medicine is to discriminate a different subtype of patients with different clinical outcomes including chances of recurrence and metastasis, we investigated the functional relationship between ENO1/MBP-1 expression and MMP-2 and MMP-9 activity levels in both tissues and sera of breast cancer patients. We focused on the clinical relevance of ENO1 and MMPs (MMP-2 and MMP-9) overexpression in breast cancer tissues: The association between the higher ENO1, MMP-2 and MMP-9 expression with a worse prognosis suggest that the elevated ENO1 and MMPs expression are promising biomarkers for breast cancer. A relationship seems to exist between MBP-1 expression and the decrease in the activity levels of MMP-9 in cancer tissues and MMP-2 in sera. Moreover, the sera of breast cancer patients grouped for MBP-1 expression differentially induced, in vitro, cell proliferation and migration. Our findings support the hypothesis of patient's stratification based on ENO1, MBP-1 and MMPs expression. Elucidating the molecular pathways through which MBP-1 influences MMPs expression and breast cancer regression can lead to the discovery of new management strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
39. The gelatinase MMP-9like is involved in regulation of LPS inflammatory response in Ciona robusta.
- Author
-
Cancemi, Patrizia, Di Falco, Felicia, Feo, Salvatore, Arizza, Vincenzo, and Vizzini, Aiti
- Subjects
- *
CIONA , *MATRIX metalloproteinases , *PHARYNX , *INFLAMMATION , *GELATINASES - Abstract
Abstract Matrix metalloproteinases (MMPs) are a family of endopeptidases collectively able to degrade the components of the extracellular matrix (ECM), with important roles in many biological processes, such as embryogenesis, normal tissue remodelling, angiogenesis and wound healing. New views on the function of MMPs reveal that they regulate inflammatory response and therefore might represent an early step in the evolution of the immune system. MMPs can affect the activity of cytokines involved in inflammation including TGF-β and TNF-α. MMPs are widely distributed in all kingdoms of life and have likely evolved from a single-domain protein which underwent successive rounds of duplications. In this study, we focused on the Ciona robusta (formerly known as Ciona intestinalis) MMP gelatinase homologue. Gene organization, phylogenetic analysis and 3D modeling supported the closest correlation of C. robusta gelatinase with the human MMP-9. Real-time PCR analysis and zymographic assay showed a prompt expression induced by LPS inoculation and an upregulation of enzymatic activity. Furthermore, we showed that before of the well-known increase of TGF-β and TNF-α levels, a MMP-9like boost occurred, suggesting a possible involvement of MMP-9like in regulating inflammatory response in C. robusta. Highlights • The Ciona genome reveals one gene for the gelatinase MMPs. • Domain organization, Phylogenetic and structural analysis support the closest correlation with human MMP-9. • Cr MMP-9 like is involved in inflammatory response induced by lipopolysaccharide (LPS). • Cr MMP-9 like early boost suggests a possible involvement of MMP-9like in regulating inflammatory response in C. robusta. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
40. A phage screening method to isolate intron-containing genes in the presence of multiple processed pseudogenes.
- Author
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Feo, Salvatore, Davies, Brendan, and Fried, Mike
- Published
- 1990
41. Allergens of Parietaria judaica pollen—I. Purification and characterization of a hapten and a low molecular weight allergenic peptide
- Author
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Feo, Salvatore, Cocchiara, Roberta, and Geraci, Domenico
- Published
- 1984
- Full Text
- View/download PDF
42. Nucleotide sequence of a cDNA encoding the human muscle-specific enolase (MSE).
- Author
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Cali, Larissa, Feo, Salvatore, Oliva, Daniele, and Giallongo, Agata
- Published
- 1990
43. An improved method for the screening of YAC libraries.
- Author
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Heard, Edith, Davies, Brendan, Feo, Salvatore, and Fried, Mike
- Published
- 1989
44. The Kelch protein NS1-BP interacts with alpha-enolase/MBP-1 and is involved in c-Myc gene transcriptional control
- Author
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Perconti, Giovanni, Ferro, Arianna, Amato, Felice, Rubino, Patrizia, Randazzo, Davide, Wolff, Thorsten, Feo, Salvatore, and Giallongo, Agata
- Subjects
- *
MYC oncogenes , *GENETIC transcription , *MESSENGER RNA , *CELL growth - Abstract
Abstract: Alpha-enolase is a key glycolytic enzyme that plays a functional role in several physiological processes depending on the cellular localization. The enzyme is mainly localized in the cytoplasm whereas an alternative translated form, named MBP-1, is predominantly nuclear. The MBP-1 protein has been characterized as a c-Myc promoter binding protein that negatively controls transcription. In the present study, we identified the kelch protein NS1-BP as one of the alpha-enolase/MBP-1 partners by using a yeast two-hybrid screening. Although NS1-BP has been originally described as a protein mainly localized in the nucleus, we provide evidence that NS1-BP also interacts with actin in human cells, as reported for most kelch-containing proteins. Here we showed that alpha-enolase and MBP-1 associate with NS1-BP in vitro and in vivo by GST pull-down assays and coimmunoprecipitation experiments; subsequent immunofluorescent staining confirmed colocalization of the proteins within the cells. Furthermore, functional analyses performed by cotransfection assays revealed that NS1-BP enhances the inhibitory effect exerted by MBP-1 on c-Myc promoter. In mammalian cells, the overexpression of both proteins resulted in an increased repression of basal c-Myc transcription and consistently affected the steady state levels of endogenous c-Myc mRNA. These findings further support the distinct roles of alpha-enolase and its MBP-1 variant in maintaining cell homeostasis. Moreover, our data suggest a novel function for NS1-BP in the control of cell proliferation. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
45. Chemically modified tetracyclines induce cytotoxic effects against J774 tumour cell line by activating the apoptotic pathway
- Author
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D'Agostino, Pietro, Ferlazzo, Viviana, Milano, Salvatore, La Rosa, Marzia, Di Bella, Gloria, Caruso, Rosalba, Barbera, Caterina, Grimaudo, Stefania, Tolomeo, Manlio, Feo, Salvatore, and Cillari, Enrico
- Subjects
- *
TETRACYCLINES , *APOPTOSIS , *MACROPHAGES - Abstract
Here, we have studied the effects of chemically modified tetracyclines (CMTs) on apoptosis both at the level of the cytoplasmic proteolytic caspase cascade, and on Bcl-2 and c-myc mRNA expression in the J774 macrophage cell line. The results indicate that CMTs induce morphological changes consistent with apoptotic events, as clearly demonstrated both by the acridine orange and ethidium bromide staining, and by TUNEL and fragmentation ELISA assays. Furthermore, the analysis of the cell cycle by flow cytometry shows an evident apoptotic sub-G0G1 peak, without important modifications in the cell cycle distribution. CMTs induce programmed cell death (PCD) in a dose-dependent manner and CMT-8 is the strongest among them. CMT-1 and CMT-8 activate mainly caspase-8 as attested by the inhibitory effects of Z-VAD-fmk and Z-IEDT-fmk on CMT-induced apoptosis. Part of CMT-induced PCD is due to the activation of caspase-9, since it is reduced by the specific caspase-9 inhibitor, Z-LEHD-fmk. Besides, CMTs increase Bcl-2 and c-myc mRNA expression. Collectively, these data indicate that CMTs are potentially anti-tumour agents, since they strongly trigger apoptosis both activating the proteolytic system of the caspase family and modulating genes involved in PCD regulation. [Copyright &y& Elsevier]
- Published
- 2003
- Full Text
- View/download PDF
46. Tongue stretching: technique and clinical proposal.
- Author
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Buscemi A, Coco M, Rapisarda A, Frazzetto G, Di Rosa D, Feo S, Piluso M, Presente LP, Campisi SS, and Desirò P
- Subjects
- Adult, Deglutition physiology, Head physiology, Humans, Infant, Newborn, Posture physiology, Tongue physiology, Tongue Diseases
- Abstract
Objectives: The tongue is an organ with multiple functions, from sucking to phonation, from swallowing to postural control and equilibrium. An incorrect position or mechanics of the tongue can causes sucking problems in the newborn or atypical swallowing in the adult, with repercussions on the position of the head and neck, up to influencing upright posture and other problems. Tongue dysfunctions are quite frequent (10-15%) in the population. For the manual therapist, this frequency indicates one to two subjects every 30 patients. Exercises have been proposed to improve the tone and strength of the swallowing muscles but the results are not so clear in the literature. The aim of this study is to describe and provide a tongue muscle normalization technique that helps the manual therapist in the treatment of problems related to it., Methods: The literature has been investigated through pubmed, Google scholar of the last 10 years, the keywords used and combined with the Boolean operators AND and OR, are: "tongue, tongue habits, tongue diseases, taste disorder, neck pain, posture, postural balance, atypical swallowing, muscle stretching exercise, tissue expansion, soft tissue therapy, osteopathic manipulative treatment"., Results and Conclusions: The technique is possible to be executed even in a sitting position, in the case the patient is unable to assume a supine position, the subject should provides immediate feedback that allows the therapist to understand if the technique has been correctly executed. The simplicity of execution and application of the technique makes it a possible and immediate therapeutic tool in the clinical setting., (© 2021 Andrea Buscemi et al., published by De Gruyter, Berlin/Boston.)
- Published
- 2021
- Full Text
- View/download PDF
47. Anti-Inflammatory Action of Heterogeneous Nuclear Ribonucleoprotein A2/B1 in Patients with Autoimmune Endocrine Disorders.
- Author
-
Coppola A, Cancemi P, Tomasello L, Guarnotta V, Pitrone M, Failla V, Cillino S, Feo S, Pizzolanti G, and Giordano C
- Abstract
Our previous studies documented that human fibroblast-limbal stem cells (f-LSCs) possess immunosuppressive capabilities, playing a role in regulating T-cell activity. This study highlights the molecular activities by which human f-LSCs can attenuate the inflammatory responses of self-reactive peripheral blood mononuclear cells (PBMCs) collected from patients with autoimmune endocrine diseases (AEDs). Anti-CD3 activated PBMCs from twenty healthy donors and fifty-two patients with AEDs were cocultured on f-LSC monolayer. 2D-DIGE proteomic experiments, mass spectrometry sequencing and functional in vitro assays were assessed in cocultured PBMCs. We identified the downmodulation of several human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) isoforms in healthy and AED activated PBMCs upon f-LSC interaction. The reduction of hnRNPA2/B1 protein expression largely affected the cycling ki67
+ , CD25+ , PD-1+ reactive cells and the double marked CD8+ /hnRNPA2B1+ T cell subset. Anti-PD1 blocking experiments evoked hnRNPA2/B1 overexpression, attributing putative activation function to the protein. hnRNPA2/B2 transient silencing inverted immunopolarization of the self-reactive PBMCs from AEDs toward a M2/Th2-type background. Pharmacological inhibition and co-immunoprecipitation experiments demonstrated the involvement of NF-ĸB in hnRNPA2/B activity and turnover. Our data indicate cardinal involvement of hnRNP A2/B1 protein in peripheral mechanisms of tolerance restoration and attenuation of inflammation, identifying a novel immunoplayer potentially targetable in all AEDs., Competing Interests: The authors declare that they have no conflict of interest.- Published
- 2019
- Full Text
- View/download PDF
48. A multiomics analysis of S100 protein family in breast cancer.
- Author
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Cancemi P, Buttacavoli M, Di Cara G, Albanese NN, Bivona S, Pucci-Minafra I, and Feo S
- Abstract
The S100 gene family is the largest subfamily of calcium binding proteins of EF-hand type, expressed in tissue and cell-specific manner, acting both as intracellular regulators and extracellular mediators. There is a growing interest in the S100 proteins and their relationships with different cancers because of their involvement in a variety of biological events closely related to tumorigenesis and cancer progression. However, the collective role and the possible coordination of this group of proteins, as well as the functional implications of their expression in breast cancer (BC) is still poorly known. We previously reported a large-scale proteomic investigation performed on BC patients for the screening of multiple forms of S100 proteins. Present study was aimed to assess the functional correlation between protein and gene expression patterns and the prognostic values of the S100 family members in BC. By using data mining, we showed that S100 members were collectively deregulated in BC, and their elevated expression levels were correlated with shorter survival and more aggressive phenotypes of BC (basal like, HER2 enriched, ER-negative and high grading). Moreover a multi-omics functional network analysis highlighted the regulatory effects of S100 members on several cellular pathways associated with cancer and cancer progression, expecially immune response and inflammation. Interestingly, for the first time, a pathway analysis was successfully applied on different omics data (transcriptomics and proteomics) revealing a good convergence between pathways affected by S100 in BC. Our data confirm S100 members as a promising panel of biomarkers for BC prognosis., Competing Interests: CONFLICTS OF INTEREST Authors declare no conflicts of interest.
- Published
- 2018
- Full Text
- View/download PDF
49. Anticancer activity of biogenerated silver nanoparticles: an integrated proteomic investigation.
- Author
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Buttacavoli M, Albanese NN, Di Cara G, Alduina R, Faleri C, Gallo M, Pizzolanti G, Gallo G, Feo S, Baldi F, and Cancemi P
- Abstract
Silver nanoparticles (AgNPs), embedded into a specific polysaccharide (EPS), were biogenerated by Klebsiella oxytoca DSM 29614 under aerobic (AgNPs-EPS
aer ) and anaerobic conditions (AgNPs-EPSanaer ). Both AgNPs-EPS matrices were tested by MTT assay for cytotoxic activity against human breast (SKBR3 and 8701-BC) and colon (HT-29, HCT 116 and Caco-2) cancer cell lines, revealing AgNPs-EPSaer as the most active, in terms of IC50, with a more pronounced efficacy against breast cancer cell lines. Therefore, colony forming capability, morphological changes, generation of reactive oxygen species (ROS), induction of apoptosis and autophagy, inhibition of migratory and invasive capabilities and proteomic changes were investigated using SKBR3 breast cancer cells with the aim to elucidate AgNPs-EPSaer mode of action. In particular, AgNPs-EPSaer induced a significant decrease of cell motility and MMP-2 and MMP-9 activity and a significant increase of ROS generation, which, in turn, supported cell death mainly through autophagy and in a minor extend through apoptosis. Consistently, TEM micrographs and the determination of total silver in subcellular fractions indicated that the Ag+ accumulated preferentially in mitochondria and in smaller concentrations in nucleus, where interact with DNA. Interestingly, these evidences were confirmed by a differential proteomic analysis that highlighted important pathways involved in AgNPs-EPSaer toxicity, including endoplasmic reticulum stress, oxidative stress and mitochondrial impairment triggering cell death trough apoptosis and/or autophagy activation., Competing Interests: CONFLICTS OF INTEREST Authors declare no conflicts of interest.- Published
- 2017
- Full Text
- View/download PDF
50. Pro-invasive stimuli and the interacting protein Hsp70 favour the route of alpha-enolase to the cell surface.
- Author
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Perconti G, Maranto C, Romancino DP, Rubino P, Feo S, Bongiovanni A, and Giallongo A
- Subjects
- Cell Line, Tumor, Cell Movement genetics, Gene Expression Regulation, Neoplastic, Humans, Lipopolysaccharides immunology, Phosphopyruvate Hydratase genetics, Protein Binding, Cell Membrane metabolism, HSP70 Heat-Shock Proteins metabolism, Phosphopyruvate Hydratase metabolism
- Abstract
Cell surface expression of alpha-enolase, a glycolytic enzyme displaying moonlighting activities, has been shown to contribute to the motility and invasiveness of cancer cells through the protein non-enzymatic function of binding plasminogen and enhancing plasmin formation. Although a few recent records indicate the involvement of protein partners in the localization of alpha-enolase to the plasma membrane, the cellular mechanisms underlying surface exposure remain largely elusive. Searching for novel interactors and signalling pathways, we used low-metastatic breast cancer cells, a doxorubicin-resistant counterpart and a non-tumourigenic mammary epithelial cell line. Here, we demonstrate by a combination of experimental approaches that epidermal growth factor (EGF) exposure, like lipopolysaccharide (LPS) exposure, promotes the surface expression of alpha-enolase. We also establish Heat shock protein 70 (Hsp70), a multifunctional chaperone distributed in intracellular, plasma membrane and extracellular compartments, as a novel alpha-enolase interactor and demonstrate a functional involvement of Hsp70 in the surface localization of alpha-enolase. Our results contribute to shedding light on the control of surface expression of alpha-enolase in non-tumourigenic and cancer cells and suggest novel targets to counteract the metastatic potential of tumours.
- Published
- 2017
- Full Text
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