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Abstract The almost complete absence of regulations to protect invertebrates is a common condition in legal systems, including the European one, especially when it comes to invertebrates intended for human consumption. Thus, in the vast majority of cases, edible invertebrates do not receive even the most basic protection at slaughter. Despite recent research indicating that invertebrates are capable of feeling pain and stress, the humane step of stunning is not used on them. This is also the case for land snails, which are gastropod invertebrates whose consumption has now reached significant levels, already involving tonnes and that is expected to increase significantly as edible snail farming becomes more popular as a relatively low-cost, easy-to-perform, and sustainable alternative animal husbandry, thereby making land snails an increasingly economically important species. This paper presents and investigates a proposed stunning method based on the immersion of mollusks in CO2-supplemented and refrigerated water that could be used in the snail meat production chain to reduce the slaughter suffering of millions of these invertebrates. To this end, body condition descriptors (hemolymph parameters) in snails were determined before and after CO2 treatment in cold water, while generating useful data for defining a preliminary set of reference intervals for basal values.

Bayesian networks are possibly the most successful graphical models to build decision support systems. Building the structure of large networks is still a challenging task, but Bayesian methods are particularly suited to exploit experts’ degree of belief in a quantitative way while learning the network structure from data. In this paper details are provided about how to build a prior distribution on the space of network structures by eliciting a chain graph model on structural reference features. Several structural features expected to be often useful during the elicitation are described. The statistical background needed to effectively use this approach is summarized, and some potential pitfalls are illustrated. Finally, a few seminal contributions from the literature are reformulated in terms of structural features.

This work was developed within the Project Metodologie e sistemi integrati per la qualificazione di produzioni orticole del Fucino (Methodologies and integrated systems for the classification of horticultural products in the Fucino plateau), sponsored by the Italian Ministry of Education, University and Research, Strategic Projects, Law 448/97. Agro-system managing, especially if necessary to achieve high quality in speciality crops, requires knowledge of main features and intrinsic variability of climate. Statistical models may properly summarize the structure existing behind the observed variability, furthermore they may support the agronomic manager by providing the probability that meteorological events happen in a time window of interest. More than 30 years of daily values collected in four sites located on the Fucino plateau, Abruzzo region, Italy, were studied by fitting Bayesian generalized linear models to air temperature maximum /minimum and rainfall time series. Bayesian predictive distributions of climate variables supporting decision-making processes were calculated at different timescales, 5-days for temperatures and 10-days for rainfall, both to reduce computational efforts and to simplify statistical model assumptions. Technicians and field operators, even with limited statistical training, may exploit the model output by inspecting graphs and climatic profiles of the cultivated areas during decision-making processes. Realizations taken from predictive distributions may also be used as input for agro-ecological models (e.g. models of crop growth, water balance). Fitted models may be exploited to monitor climatic changes and to revise climatic profiles of interest areas, periodically updating the probability distributions of target climatic variables. For the sake of brevity, the description of results is limited to just one of the four sites, and results for all other sites are available as supplementary information.

The gray wolf (Canis lupus) expanded its distribution in Europe over the last few decades. To better understand the extent to which wolves could re-occupy their historical range, it is important to test if anthropization can affect their fitness-related traits. After having accounted for ecologically relevant confounders, we assessed how anthropization influenced i) the growth of wolves during their first year of age (n = 53), ii) sexual dimorphism between male and female adult wolves (n = 121), in a sample of individuals that had been found dead in Italy between 1999 and 2021. Wolves in anthropized areas have a smaller overall variation in their body mass, during their first year of age. Because they already have slightly higher body weight at 3-5 months, possibly due to the availability of human-derived food sources. The difference in the body weight of adult females and males slightly increases with anthropization. However, this happens because of an increase in the body mass of males only, possibly due to sex-specific differences in dispersal and/or to "dispersal phenotypes". Anthropization in Italy does not seem to have any clear, nor large, effect on the body mass of wolves. As body mass is in turn linked to important processes, like survival and reproduction, our findings indicates that wolves could potentially re-occupy most of their historical range in Europe, as anthropized landscapes do not seem to constrain such of an important life-history trait. Wolf management could therefore be needed across vast spatial scales and in anthropized areas prone to social conflicts.