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The unmanned aerial vehicles (UAVs) are widely used for agricultural monitoring due to reduce the cost and time of crop monitoring via the acquisition of images with high spatial-temporal resolution. The normalized difference vegetation index (NDVI) is the most widely studied and used for mapping crop growth. A relatively expensive multispectral sensor is required to produce an NDVI map. The visible vegetation indices (VIs) derived from UAV images showed potential capabilities for predicting crop growth. The purpose of this paper is to evaluate the RGB indices to monitor the growth of the rice crop. The images were obtained from the study area by DJI PM4 multispectral UAV. The multispectral images were used to calculate NDVI as a reference vegetation index and different RGB indices were implemented and compared with the reference index. The results showed that RGB indices can be used as the vegetation index in the case of unavailable multispectral images.

Environmental and human health requirements necessitate replacing chemical herbicides with natural ones for weed control. Allelopathy is a form of interaction among plants that may assist in producing natural herbicides, but less research has been conducted in this important area. Allelopathic effects of extracts of five drought-tolerant plant species widely used in urban landscapes were examined using five completely randomized design experiments with four replications. The species were Rosmarinus officinalis, Lavandula officinalis, Salvia sclarea, Atriplex halimus, and Atriplex canescens. The plant species' soluble extracts were prepared at 0, 2.5, 5, and 7.5%. The seeds' germination percentage and rates of Taraxacum officinale, Conyza canadensis, and Tragopogon major were measured daily after being affected by these extracts. Increasing the concentrations of all the extract types was associated with germination percentages and rate declines. The most outstanding allelopathic effects were related to Atriplex halimus and Salvia sclarea extracts, respectively. Tragopogon major was considered the most sensitive weed species in reducing its germination rate when affected by the landscape plant extracts, except for R. officinalis extract. The reduction in germination percentage of T. major and C. canadensis under A. halimus extract compared to the control was the highest. A. halimus and S. sclarea extracts can be used as natural weed controllers for important weeds such as Taraxacum officinale.

Introduction Turfgrasses, as one of the important components of urban landscapes, have played a vital role in this regard. However, the main problems of turfgrass development are the cost of turfgrass seeds, maintenance costs such as moving, as well as the high water requirement in arid and semi-arid regions. Thus, the reduction of lawn culture in the landscape is one of the decision has taken in parks and green spaces organization of Tehran, Isfahan and even Mashhad. For over ten years, researchers have been looking for alternatives to conventional grass lawns to reduce the high cost of maintenance especially irrigation cost in urban landscapes. According to many researches, one of the potential ground cover alternatives that might be used instead of turfgrass is White Clover. Clover (Trifolium spp.) from Fabaceae family is a genus of about 300 species. Materials and Methods The aim of this experiment was to compare three common turfgrasses with two clover varieties as turf replacement to reduce landscape maintenance cost specially moving cost. So, some growth and qualitative factors of clover lawn: Trifolium repens var. Calway and Trifolium repens var. Pipolina (micro clover) and three turfgrasses of Festuca arundinacea L. and Lolium perenne L. and commercial sport turf mixture (from NAk-Nederland Ltd.) were compared together. This research was conducted in randomized complete block design with three replications in the research field of the Department of Horticultural Science and Landscape Engineering‚ Faculty of Agricultural‚ Ferdowsi University of Mashhad‚ Mashhad‚ Iran, during 2016 and 2017. The site (59º 38′ E and 36º 16 ′ N; elevation 989 m) is located in an arid and semi- arid region with mean annual rainfall 233.8 mm and long term averages of maximum and minimum temperature are 22.5 ºC and 9.3 ºC‚ respectively. Turfgrass plots were established by directly sowing the seeds in April, 2016. The planting rate of the seedling considering their pure live seeds (PLS) were 45 g.m-2 for Lolium perenne, 34 g.m-2 for Festuca arundinacea, 6.5 g.m-2 for Trifolium repens, 5.5 g.m-2 for micro clover and 39 g.m-2 for commercial mixture of sport turf. The plots were 1 m2 (1m×1m) in size and were prepared after plowing and leveling the soil. The seeds were hand sown and covered with a thin layer of leaf compost and sand. Results and Discussion According to the results in the first evaluation of emergence percentage, Lolium perenne had the highest emergence percentage. After 36 days from culture, all plants had 92-98 % coverage which did not have any significant difference among grasses with clover lawns. In terms of density, uniformity and weed density, there was no significant difference between the studied plants. In the other hands, white clover showed the best quality after clipping in both years. At the point of growth index, which have done in this experiment by measuring height and dry weight of clipping, grass lawns had the higher growth index in compered to clover lawn in both years. Festuca arundinacea and Lolium perenne had the highest growth index and white and micro clover had the lowest growth. Also, with air warming, Festuca arundinacea became dominated plant in height and clipping dry weight. In July, Festuca arundinacea produced the highest clipping dry weight by 40 gr.m-2, Lolium perenne (19.52), commercial sport turf (15.68), white clover (6.24) and micro clover (0.36) gr.m-2. Conclusion One of the problems of landscape is reduction of maintenance costs such as mowing. So, the low growth of white and micro clover is a positive factor in reduction of the moving costs than turfgrasses. Also, the coverage and proper density of clover lawns are similar to grass lawns and did not have a significant difference with them. The character of low growth can be effective in reduction of irrigation costs in white and micro clover, which requires more research and examination in future. This is a positive point in the white clover and micro clover that do not need to move or cut every months. Almost, clover moving is recommended for removing their flower. Therefore, it is recommended to use white and micro clover as a replacement with less maintenance costs in landscape. Finally, the results of cultivation of grass and clover as monoculture in this study can be used to produce clover-grass mixtures suitable for the climate of our country (arid and semi-arid regions), instead of importing turf mixtures from European countries.

High temperatures, low average rainfall, drought, and high evapotranspiration are limiting factors in arid lands. Therefore, when constructing landscapes (green spaces) in these regions, strategies should be developed to mitigate these climatic influences. One practical strategy is utilizing different types of mulches on the surface of the soil. This study was conducted as a randomized complete block design experiment with four replications during 2014 and 2015 to examine the performance of organic and inorganic mulches as non-living mulches and ground cover plants as living mulches. Ground cover plants were Carpobrotus sp., Potentilla reptans, Vinca minor, Frankenia sp. and a mixed turfgrass. Non-living mulches were turfgrass clippings, wood chips, sawdust, gravel, rubble and scoria (volcanic rock). Bare soil was used as the control treatment. The results demonstrated the application of mulches could modify soil temperature at 5 and 15 cm depths in different seasons of the year. The living mulches especially Carpobrotus sp. and turfgrass reduced the temperature more than the non-living mulches. The soil covered with sawdust and wood chips preserved soil moisture content over the soil covered with other types of mulches. It would appear the selected mulches could decrease the irrigation intervals through increased water holding capacity of the soil. The outcomes of this research could assist landscape managers operating in extreme climate conditions of arid and semi-arid regions to advance the management of soil moisture and temperatures with the objective to improve sustainability.

Creating sustainable landscapes especially in arid climates is a significant challenge. To achieve sustainable landscapes, some strategies such as using native plants and soil mulches have been suggested. This study aimed to examine the quality and growth response of the native tall fescue (Festuca arundinacea Schreb.) compared with the corresponding commercial cultivar (Festuca arundinacea ‘Jaguar’) under different planting mulches in their planting and early growth stage in arid climate Mashhad city located in the northeast of Iran. Seeds of commercial cultivar and native species of Festuca arundinacea were sown in a sandy loam texture soil and four mulch treatments including vermicompost, leaf compost, cow manure and sand as control, were applied to cover the seeds. The result showed that the native species had greener color and narrower leaves compared to the commercial cultivar but its other NTEP (National Turfgrass Evaluation Program) traits including density, uniformity, establishment, softness of grass surface, quality after clipping, and total quality was lower than that in the commercial cultivar. Using vermicompost as the planting mulch (top dressing or cover mulch at the time of planting of the turfgrass) significantly improved all of the traits in the native species. Vermicompost increased the percentage of grass coverage, clipping dry weight, and the plant height and decreased the leaf width of plants and number and percentage of weeds. Utilizing native species of tall fescue with vermicompost as the planting mulch can promise as quality lawn as their commercial cultivar for native urban landscaping in Iran.

Currently, the idea of ​​integrating nature and buildings through green roofs and green walls has been considered as a sustainable strategy in cities. However, studies on the substrates which allow the plants to fully establish in these systems are still lacking. It is also a challenge to find the substrates which provide the plants with proper growth, nutrition, and environmental sustainability. This study aimed to evaluate the performance of Frankenia thymifolia in four types of growing media (30% cocopit + 65% perlite + 5% vermicompost, 30% soil + 65% perlite + 5% vermicompost, 30% mushroom compost + 65% perlite + 5% vermicompost and common soil used in the green spaces as the control) in growing conditions of the external green walls. The experiment was conducted as a split-plot in a randomized complete block design with three replications. The main factor was different seasons of the year in four levels and the second factor was the substrates in four levels. The effect of different substrates on thermo-dynamic factors of the substrates and on morphological, qualitative, and water use efficiency characteristics of the plants were significant at the 5% probability level. The results showed that the temperature and humidity of the substrates containing organic compounds were higher than those of the soil. Also, plant height, number of nodes, percentage of coverage, and water use efficiency of the plants were higher in the growing medium containing mushroom compost. Having a high water use efficiency and keeping its qualitative characteristics to an acceptable level in the summer and spring, Frankenia was found to be a suitable species in these seasons. However, due to the cold-induced deleterious effects in winter on these characteristics, it was not a suitable plant species for external green wall systems in climatic conditions of the winter season of Mashhad. Based on this studychr('39')s results, we suggest adding washed mushroom compost to the usual green wall substrate. Frankenia can also be considered a suitable plant for external green walls in spring, summer, and autumn in similar weather conditions to Mashhad.

Considering a large number of cities located in arid and semi-arid climatic regions of the world with limited water resources, reducing water consumption and maintenance costs is an important research and implementation priority in urban landscaping. In other words, reducing the high costs of irrigation, increasing water use efficiency and reducing weed competition are important factors in achieving sustainable green spaces in arid and semiarid regions. Application of mulches is one of the suggested strategies for maintaining bed moisture and weed control. In this research, the effects of organic and inorganic mulches on the performance of the flowering plant of Blanket flower (Gaillardia sp.) were investigated in the arid climate city of Mashhad located in the northeast of Iran. The experiment was conducted as a randomized complete block design with three replications. The four mulch treatments included wood chips, scoria, pine leaves, polyethylene as a layer mulch, and no mulch as the control. The polyethylene mulch inhibited the weed growth up to 100%, and other mulch types also significantly reduced the percentage of the weed coverage (p≤0.01). Application of pine leaves delayed the flowering for seven days while polyethylene caused an early flowering to six days compared to the control. Using different mulch types in water shortage conditions of urban landscapes in arid and semi-arid regions is recommended.

Application of green walls still has not attracted interest among the contractors and people, and this can be due to poor growing performance of the plants on these systems. This study investigated the effect of four growing media types (30% cocopeat + 65% perlite + 5% vermicompost, 30% soil + 65% perlite + 5% vermicompost, 30% mushroom compost + 65% perlite + 5% vermicompost and regular soil as the control) on the performance of three plant species (Frankenia thymfolia, Vinca minor, and Potentilla sp.) on green walls under arid climate of Mashhad city in Iran. In the control growing medium with no organic matter, Frankenia maintained the highest moisture and the lowest temperature compared to the other growing media types. The growing media did not affect the root and shoot fresh and dry weight and leaf relative water content of the Frankenia. Vinca minor in the cocopeat and mushroom compost produced the highest root fresh and dry weight; however, changing to the growing media types enriched with organic matter content did not affect the shoots fresh and dry weight of this plant type. The growing media containing organic matters compared to the control growing medium, improved relative water content of the Potentilla leaves. The growing media containing organic matters, especially mushroom compost, had positive effects on improving the growth performance of the green walls, through maintaining the moisture content of the media, and thus the relative water content of the leaves, which enhanced vitality of the plants.

Kinetic models are the most important instruments for predicting and evaluating the performance of flotation circuits. To determine the kinetic order and rate of flotation of a gilsonite sample, flotation experiments were carried out in both rougher and cleaner stages. Experiments conducted using the combinations of petroleum-MIBC, gas oil-pine oil, and one test without any collector and frother. Five first order kinetic models were applied to the data obtained from the flotation tests by using the Matrix Laboratory software. Statistical analysis showed that the classic first order model perfectly matched the rougher and cleaner results performed using petroleum-MIBC combination. The kinetic constants (k) were calculated as 0.04 (s-1) and 0.01 (s-1) for the rougher and cleaner, respectively. Rougher and cleaner tests without collector and frother also matched with the modified gas/solid adsorption and rectangular models with the k values of 0.05 (s-1), and 0.01 (s-1), respectively. The relationship between flotation rate constant, maximum combustible recovery and particle size were also studied. The results showed that the maximum flotation combustible recovery and flotation rate were obtained with an intermediate particle size both in the rougher and cleaner flotation processes. The combustible recovery and flotation rate in the rougher flotation process were higher than that in the cleaner flotation process.

Creating water-conserving turfgrasses using superabsorbents in soil is a challenge especially in arid environments because their related soil and plant’s behavior is still less known. This research investigated soil moisture content and physiological characteristics of Lolium perenne L. ‘Chadegan’ (perennial ryegrass) and Festuca arundinacea (tall fescue), in the absence (control treatment) or presence of four superabsorbent types. The superabsorbents were Zeolite (zero and 10% wt), Bentonite (zero and 6%wt), Aquasorb (zero and 6 g/m2) and Boloorab (zero and 50 g/m2). The experiment was factorial based on a randomized complete block design with four replications. A one-month stop on irrigation occurred after the first five-month full irrigation regime to simulate drought stress conditions. The results showed tall fescue had higher leaf relative water content, lower relative electrolyte leakage and lower relative saturation deficit than that in perennial ryegrass after applying the drought stress. However, tall fescue had higher chlorophyll content than this factor in the ryegrass. Aquasorb and then Bentonite were able to improve the fresh weight and dry weight in roots and shoots under drought stress conditions compared to the control treatment. Using Aquasorb was associated with better physiological characters in the turfgrasses and higher water content in the soil. Overall, under drought stress conditions, planting tall fescue in a soil containing Aquazorb promised a better quality turfgrass for urban green spaces.

Application[Editor 321] of biotic growth regulators (e.g. humic components) and appropriate mulches is recommended to improve turfgrass quality especially in arid andsemi-arid climate zones. However, limited number of studies have investigated their effect on lawn establishment. To investigate the effect of humic acid (HA) and selected mulches on characteristics of Festuca arundinacea in its planting stage, a factorial experiment based on a completely randomized block design with three replications was performed. The first factor was mulch types including vermicompost, leaf compost, cow manure,[Editor 322] and sand (control) which were used to cover the seeds. The second factor was a HA solution (100 ml l-1) sprayed monthly over the period of the experiment. Plant height, fresh and dry weight of lawn clippings, photosynthetic index, leaf texture, and overall turfgrass quality were measured. Spraying HA significantly improved the measured factors except the dry weight and photosynthetic index of the plants. Among the mulches, vermicompost provided better impressions on improving the characteristics of this turfgrass species including 48% increase in fresh weight, 18% increase in height, 48% increase in total quality, and 10% reduction in leaf width of the turfgrass. This research can assist in developing knowledge for having high quality lawns in urban landscapes of arid and semi-arid environments.

Petroleum coke contains high amounts of carbon and is used in the manufacturing of anode electrodes for the aluminum extraction. In the process of aluminum production, some particles separate from anodes as waste which contain petroleum coke. Therefore, recycling and processing of these petroleum coke particles is the subject of this study. The ash content reduced to 31% and 13% in the jig and shaking table concentrate, respectively. These two steps were considered as the pre-processing methods and heavy media separation was used to decrease the ash content much more. Finally, flotation was performed in order to purify the particles with the size of less than one millimeter.

Developing strategies to tackle water stress in lawns in arid climate regions is significantly important. In such conditions, using growing media amendments as well as native plants can relieve drought stress in different growing stages of the plants. This study was carried out to investigate the effects of some types of soil amendments on the emergence and early seedling establishment of tall fescue (Festuca arundinecea Schreb ‘GazBorkhar ecotype’) under water stress conditions. The experiment was performed in a factorial trial based on a completely randomized design with three replications. The first factor, water stress, was applied based on field capacity (FC) at three levels (80% FC (control), 60% FC and 40% FC). The second factor, growing media amendments, was applied at nine levels of municipal solid waste (MSW), vermicompost (5% and 10% w/w), Stockosorb® (1% and 3% w/w) or zeolite superabsorbent (4% and 8% w/w) which were mixed with loam soil, and the loam soil was used as a control treatment. Theresults showed that using an irrigation system at 60% FC during seed emergence and early seedling growth did not reduce the quality of the turf grass, and also, in many cases, led to a higher quality lawn compared to the control, leading to water saving in turf grass management. Using MSW and manure vermicompost as soil amendments could maintain soil moisture and water supplement for the improvement of morphological, physiological and visual quality of this ecotype of Festuca arundinacea Schreb.

Mulches are relatively new landscape components and are becoming recognized for their environmental and aesthetic outcomes on urban landscapes especially in arid environments. However, the effects of mulches on landscape plants have not been extensively discussed. This study examined the effects of organic and inorganic mulches on the performance of a widely used herbaceous drought tolerant flowering plant, Zinnia elegans, toward low maintenance landscaping. This study was designed as a randomized complete block design with three replications. Four widely commercially available and utilized mulches, including wood chips, pine needles, scoria (volcanic stone) and black polyethylene were used as the treatment mulches. The study contained plots with only bare soil as the control. The results showed that utilizing the selected mulches demonstrated positive effects on the plant growth, and increased the fresh and dry weight of shoots (p

In this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Escherichia coli bacteria transformed using pBI121 plasmid and were grown on the selection media containing kanamycin. MNPs were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). Finally, the overall efficiency of different MNPs (Fe3O4, Fe3O4/SiO2, Fe3O4/SiO2/TiO2) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication “Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction” (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1].

Festuca ovina L. is an important ornamental species used in urban landscapes and pastures reclamation. However, low resistance of this species to salt stress has hindered its extended application in low-water and salty regions. To study the effects of calcium on reducing salt stress injuries in seed germination and early growth of this species, a factorial experiment based on completely randomized design with three replications was conducted. The salt levels included NaCl solutions (5, 10, 15 and 20 dS/m). Calcium source was CaCl2 with concentrations of 0, 10, 20 and 30 mmol+/L. Germination percentage and mean germination time were significantly affected by interaction of salinity and calcium. As salinity increased, mean germination time increased. However, germination percentage, shootlet length, rootlet length, shootlet fresh and dry weight, rootlet fresh and dry weight and seed vigour index decreased following the increasing salinity. Concentrations of 10 and 20 mmol+/L CaCl2 had significant effects on reducing salt injuries on seed germination of Festuca ovina. This finding was even prominent in higher salinity levels of 15 and 20 dS/m. Therefore, to facilitate using this species in urban landscapes or in pastures, pre-treatment of the seeds using 10 and 20 mmol+/L CaCl2 is recommended.

Determination of growth rate and planting space of trees in urban spaces is needed for the planting design programs and land scape management. To determine the growth rate of two tree species, Chinaberry (Melia azedarach) and Mulberry (Morus alba), data was collected on different dimensions of these trees including, tree height, tree crown height and tree crown diameter at various ages at Pardis campus of Ferdowsi University of Mashhad during their growth. Regression analysis of these dimensions as a dependent variable and age as an independent variable produced different models that can be used for estimating the growth of the measured dimensions of these two species. The results showed that the highest regression coefficient among the models was linear, polynomial and exponential function, but logarithmic types were selected as the most acceptable model for estimating the growth of measured dimensions in the two tree species of Chinaberry (tree height with r2=0.87, crown height with r2=0.67, crown diameter with r2=0.67) and Mulberry (total height with r2=0.60, crown height with r2=0.40, crown diameter with r2=0.65). Also, in both species, the most increase in growth rate of measured dimensions for the 15 years old trees was found in crown diameter.

Zirconia (ZrO2) nanopowder was synthesized using sucrose and fructose as a chelating agent from zirconium hydroxide. The synthesized powders were characterized by X-ray diffraction (XRD), simultaneously thermal analysis (STA), BET surface area, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The above-experimental results showed that the synthesized powders had particle sizes in the range 40-60 nm and mean crystallite sizes of 7-8 nm. Finally, in this research, chelating agents of sucrose and fructose were compared and the obtained results demonstrated that using fructose, nanopowder of zirconia with tetragonal phase was obtained.

The reproduction and population growth parameters of Anisopteromalus calandrae (Howard) on the 4th instar larvae of Callosobruchus maculatus (Fabricius) were investigated at temperature of 25 ± 1ï°C, 20 ± 5% R.H. and a photoperiod of 16: 8 (L: D) hours. Since the parasitoid eggs are laid inside the seeds where the host larvae live, egg hatch rate (hx) was assumed to be 1 (100%). As a result of this study, the gross and net fecundity and fertility rates were determined to be 306.51 and 236.25, respectively. The average number of eggs produced per female per day was obtained 11.48. The intrinsic and finite rates of increase on the 4th instar of C. maculatus were 0.163 and 1.18, respectively. The mean generation and doubling times were 35.30 and 4.24 days, correspondingly. The gross and net reproduction rates were 123.36 and 109.49 females/female/generation, respectively. The results of this study on the stable age distribution showed that 62.3 % and 37.7 % of the population belonged to immature stages and adults, respectively. The possible application of these results for biological control of C. maculatus is discussed.

In this paper, we investigate the entanglement dynamics of a two qutrits system interacting with a spin environment. Using negativity as the entanglement measure, we study the entanglement dynamics of the system. The calculations show that in cases where the entanglement decays quickly, the environment will have a quantum phase transition. [ABSTRACT FROM AUTHOR]

In this work, a continuous wave fast axial flow RF CO2 laser has been constructed, which is pumped by a 1 kW RF generator with a 13.56 MHz frequency. In order to optimize the laser performance, the diameter of the discharge tube, electrodes width, the ratio of the width of the electrode to the tube perimeter, gas ratio, and total pressure of the gas mixture was changed over a wide range for a constant electrode length of 10 cm and then the laser output power and efficiency were measured. The maximum output power of 63.5 W, implying% a 6.3 electric efficiency, was obtained for 26 mm discharge tube diameter, electrode width to discharge tube perimeter ratio of 0.6, gas mixture, and 90 mbar total pressure..

BACKGROUND AND OBJECTIVE: One of the factors affecting dermatophyte is hormonal factors, in which atopic dermatitis is more common in adult women. Since one of the psychotic problems regarding reproductive performance of women is premenstrual syndrome, in this study, the association between premenstrual syndrome and atopic dermatitis in reproductive age women was investigated. METHODS: This case-control study was performed on 82 women aged between 15 and 49 years old in two groups of atopic dermatitis referring to Skin Clinic of Sina Hospital in Tehran and control group including healthy women for atopic dermatitis. Demographic questionnaire and (PSST) Premenstrual Symptoms Screening Test (with score 42-0) were completed and evaluated by both groups. FINDINGS: The mean age of subjects in the case group was 17.17 ± 4.45 and in the control group was 27.29 ± 8.03 years. The results showed by controlling the possible confounding factors as age, occupation and marital status the probability of having moderate or severe premenstrual syndrome in patients with atopic dermatitis is 13.26 (OR= 13.26, 95% CI: 3.31, 53.07), as compared with healthy subjects. CONCLUSION: The results showed that there is a strong relationship between moderate to severe menstrual syndrome and atopic dermatitis in women.

Coaxial couplers with high input RF power, usually operated at 500 MHz, are one of the critical components used in accelerators. To withstand high RF power, the couplers should be tested first, under identical conditions in a test stand. A waveguide coupled to a coaxial cable is used for this purpose. The parameter that indicates the amount of returned power loss is S11. By optimizing the dimensions of the proposed model this parameter can be reduced.​​ ​MATLAB program has special and powerful tools to optimize the models which are simulated in CST STUDIO SUITE. In this paper the details of how MATLAB can control CST is described. Using MATLAB to control CST, the optimization of the coaxial coupler coupling 1800 waveguide is carried out.

BACKGROUND AND OBJECTIVE: Whooping cough is an acute contagious human respiratory disease especially in infants and children and is one of the ten major causes of death from infectious diseases. Despite reducing the risk due to vaccination with killed bacteria pathogen (Bordetella pertussis), increased disease emerged in recent decades indicates re-emerging due caused by genetic diversity of bacteria and the insufficiency of current vaccines. Therefore, the design of new and effective vaccines based on common strains of the population is essential. In this study the prevalent strain Iranian B.pertussis strains has been selected for gene amplification of pertussis toxin (PTX), the most important antigen of bacteria. The toxicity of the PTX related to its enzymatic activity therefore by site directed mutagenesis, the effective residues in the active site of the enzyme has been changed and the inactive and safe recombinant was produced. METHODS: Bacterial strain used in this study to determine the genome and gene amplification was selected from pathogenic strains isolated from Iranian patients from the pertussis reference laboratory of Microbiology of the Pasteur Institute of Iran. Genomic DNA was extracted by kit, ptx- s1(the enzymatic fragment of PTX) fragments amplified and inserted in pUC18. Site directed mutagenic primers were used for substitution of Arg 9 by Lys and Glu129 by Gly. The confirmed mutant ptx- s1 (mptx-s1) was sub-cloned into expression vector pET15b. The expression of recombinant protein in E.coli BL21 was induced by 0.5mM IPTG. The expression was analyzed by SDS-PAGE and immuno-blotting. The protein was puifird by Ni-NTA affinity chromatography under denature condition. FINDINGS: Sequencing data and restriction enzyme analysis confirmed the gene constructing and mutations. The presence of a 28 kDa band in SDS-PAGE and immunoblot showed the expression of mutant PTX. The concentration of recombinant protein was 0.36 mg/ml. CONCLUSION: The Recombinant protein which was obtained in this study provided the possibility of designing acellular vaccines on the basis of important antigens of prevalent strain of B.pertussis in Iran.

This study aimed to investigate the forming limit diagrams (FLDs), mechanical properties, and bending behavior of the three kinds of sandwich composites. The core layer in all sandwich sheets consisted of polyethylene, and just the skin layers were chosen to be AA5754 or AA3105. The results showed that the sandwich sheets are an appropriate choice to be used instead of the monometallic sheets. According to the 3-point bending test results, the flexural strength of the AL 3105/Polyethylene/AL 3105 sandwich composite is about 30 percent less than the AA3105 monometallic layer. Also, the formability of the metallic sandwich composites with non-identical skin layers was studied. The results demonstrated that using one layer AA 3105 and one layer AA5754 instead of AA5754 for both skin layers, although formability decreased about 11% in the plane strain region, the cost of fabricating the composite was decreased sheet reduces almost 75%. As a result, using skin layers with the different grades of aluminum is an appropriate way to reduce the cost of fabricating the aluminum-polymer sandwich composites, in which the formability does not decrease significantly.

Background & Aims: Blood inflammatory factors have been recognized as major risk factors of cardiovascular diseases. The purpose of this study was to compare the efficacy of two methods of excessive aerobic continuous and interval training on plasma lipoproteins (TC, TG, LDL, HDL, VLDL) and serum CRP in women. Methods: A total of 20 women aged 35-45 years were randomly divided into the two groups of aerobic continuous and interval training. Subjects participated in an 8- week either aerobic continuous or interval running program (3 day / week, at 65-75% HRmax). Twenty four hours before the first training program and 24 hours after the final training session blood samples were collected in a fasted state. Results: No significant difference was found between the two groups in regard to TC, TG, LDL, HDL, VLDL and CRP levels in both pre- and post- tests. Aerobic continuous training induced significant decrease in TC, LDL, CRP and increase in HDL. Aerobic interval training induced significant decrease in TC, TG, LDL, CRP and increase in HDL too, but no significant difference was found between the two methods of training on plasma lipoproteins and serum CRP. Conclusion: Excessive aerobic continuous and interval training can have similar effects on plasma lipoproteins and serum CRP in women.

Introduction: Type 2 diabetes is a common disease in the world. Exercise has consistently been shown to improve glucose control. The purpose of this study was to compare the effect of a single-session aerobic and resistance exercise on blood glucose in women with type 2 diabetes. Methods: Twenty four women with type 2 diabetes were voluntarily and randomly selected and divided into aerobic exercise, resistance exercise and control groups. Aerobic exercise(rhythmic exercise) was performed for 40 min at 60% HRmax and resistance exercise(weight lifting) for 40 min utilizing 60% of 1-RM. Blood samples were collected before and after exercise. Results: Results of paired sample t-test showed a single-session aerobic and resistance exercise significantly decreased blood glucose(p

Background: Trichomonas vaginalis is protozoan parasite responsible for trichomoniasis and is more common in high-risk behavior group such as prostitute individuals. Interest in trichomoni­asis is due to increase one's susceptibility to viruses such as herpes, human papillomavirus and HIV. The aim of this study was to find genotypic differences between the isolates.Methods: Forty isolates from prisoners' women in Tehran province were used in this study. The random amplified polymorphic DNA (RAPD) technique was used to determine genetic differ­ences among isolates and was correlated with patient's records. By each primer the banding pat­tern size of each isolates was scored (bp), genetic differences were studied, and the genealogical tree was constructed by using NTSYS software program and UPGMA method.Results: The least number of bands were seen by using primer OPD8 and the most by using OPD3. Results showed no significant difference in isolates from different geographical areas in Iran. By using primer OPD1 specific amplified fragment with length 1300 base pair were found in only 8 isolates. All these isolates were belonged to addicted women; however, six belonged to asymptomatic patients and two to symptomatic ones.Conclusion: There was not much genetic diversity in T vaginalis isolates from three different geo­graphical areas.

Background: Trichomoniasis is an extremely common sexually transmitted infection (STI) world­wide and is associated with important public health problems, including amplification of HIV transmission. This disease is in forms of symptomatic and asymptomatic in women and may de­pend on host as well as parasite variables. Most of the studies reported from females are based on examination of vaginal secretions and urine samples by direct smear and culture in modified Dia­mond's media. The aim of this study was checking the samples, which were negative by direct smear and culture, with PCR technique.Methods: The urine samples and vaginal discharge of patients attending Gynecology Clinics of Ma­zandaran Province, Iran with different symptoms rechecked for Trichomonas vaginalis by PCR technique using primers targeting a conserved region of the beta-tubulin genes of the para­site. Data were analyzed by Epi Info software programResults: Out of 161 negative samples by direct smear and culture, seven samples (4.3%) were posi­tive by PCR technique.Conclusion: Diagnosis of trichomoniasis by PCR is a sensitive and specific method that could play important role to help the physicians for properly treatment and control of infection.

Background: Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out.Methods: Fifty-two suspected vaginal samples were collected from 2006 to 2007 in Gynecology Maryam Hospital, Tehran, Iran. All isolates were examined by microscopic, culture and PCR tech­niques. The PCR products were analyzed by RFLP and CSGE methods and two suspected samples were sequenced.Results: Trichomonas vaginalis was identified from all 52 samples. Of 52 isolates, 45 samples were successfully cultured and amplified by PCR except one. Seven were positive only by PCR. Finally, ITS1 fragment was successfully amplified in 51 of 52. CSGE analysis and PCR products di­gestion by MspI followed by sequencing showed nucleotide mutation at position 209 (C209T) of the ITS1 fragment in two (3.9%) of them.Conclusion: The results showed mutation in ITS1 fragment of T. vaginalis in two (3.9%) of Ira­nian isolates which may be related to metronidazole resistance.

The integration of artificial intelligence (AI) in radiology has brought about substantial advancements and transformative potential in diagnostic imaging practices. This study presents an overview of the current research on the application of AI in radiology, highlighting key insights from recent studies and surveys. These recent studies have explored the expected impact of AI, encompassing machine learning and deep learning, on the work volume of diagnostic radiologists. The present and future role of AI in radiology holds great promise for enhancing diagnostic capabilities, improving workflow efficiency, and ultimately, advancing patient care., Competing Interests: Disclosure The authors have nothing to disclose., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Objective: Brain metastases (BM) are the most common adult intracranial tumors, representing a significant source of morbidity in patients with systemic malignancy. Frailty indices, including 11- and 5-factor modified frailty indices (mFI-11 and mFI-5), American Society of Anesthesiologists (ASA) physical status classification, and Charlson Comorbidity Index (CCI), have recently demonstrated an important role in predicting high-value care outcomes in neurosurgery. This study aims to investigate the efficacy of the newly developed Hospital Frailty Risk Score (HFRS) on postoperative outcomes in BM patients., Methods: Adult patients with BM treated surgically at a single institution were identified (2017-2019). HFRS was calculated using ICD-10 codes, and patients were subsequently separated into low (<5), intermediate (5-15), and high (>15) HFRS cohorts. Multivariate logistic regressions were utilized to identify associations between HFRS and complications, length of stay (LOS), hospital charges, and discharge disposition. Model discrimination was assessed using receiver operating characteristic (ROC) curves., Results: A total of 356 patients (mean age: 61.81±11.63 years; 50.6 % female) were included. The mean±SD for HFRS, mFI-11, mFI-5, ASA, and CCI were 6.46±5.73, 1.31±1.24, 0.95±0.86, 2.94±0.48, and 8.69±2.07, respectively. On multivariate analysis, higher HFRS was significantly associated with greater complication rate (OR=1.10, p<0.001), extended LOS (OR=1.13, p<0.001), high hospital charges (OR=1.14, p<0.001), and nonroutine discharge disposition (OR=1.12, p<0.001), and comparing the ROC curves of mFI-11, mFI-5, ASA,and CCI, the predictive accuracy of HFRS was the most superior for all four outcomes assessed., Conclusion: The predictive ability of HFRS on BM resection outcomes may be superior than other frailty indices, offering a new avenue for routine preoperative frailty assessment and for managing postoperative expectations., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Myocardial infarction (MI) is an important driver of both morbidity and mortality on a global scale. Elucidating social inequalities may help to identify vulnerable groups as well as treatment imbalances and guide efforts to improve care for MI., Methods: All hospitalized patient-cases with confirmed MI 2005-2020 in Germany were included in the study and stratified for socioeconomic or psychosocial factors (SPF) and the impact of SPF on treatment usage and adverse in-hospital events was analyzed., Results: Overall, 4,409,597 hospitalizations of MI patients were included; of these, 17,297 (0.4 %) were coded with SPF. These patients were more often of female sex (49.4 % vs. 36.9 %, P<0.001), older (median 77.0 [IQR: 65.0-84.0] vs. 73.0 [62.0-81.0] years, P<0.001) and revealed an aggravated cardiovascular profile. Although SPF were independently associated with increased usage of cardiac catheterization (OR 1.174 [95 %CI 1.136-1.212]) and percutaneous coronary intervention (OR 1.167 [95 %CI 1.130-1.205]), they were accompanied by higher risk for a prolonged length of in-hospital stay >7 days (OR 1.236 [95 %CI 1.198-1.276]) and >10 days (OR 1.296 [95 %CI 1.254-1.339]). While SPF were associated with increased risk for deep venous thrombosis and/or thrombophlebitis (OR 1.634 [95 %CI 1.427-1.870]), pulmonary embolism (OR 1.337 [95 %CI 1.149-1.555]), and acute renal failure (OR 1.170 [95 %CI 1.105-1.240), these SPF were inversely associated with in-hospital case-fatality (OR 0.461 [95 %CI 0.433-0.490])., Conclusions: This study demonstrates that SPF in hospitalized MI patients have significant impacts on treatments and outcomes. Fortunately, our data did not revealed an underuse of interventional treatments in MI patients with SPF., Competing Interests: Conflicts of interest OH, SA-K, PL, VHS, FK-A, DG, KP, PW, KL, AD, TM and KK report no conflict of interests. TM is PI of the DZHK (German Center for Cardiovascular Research), Partner Site Rhine-Main, Mainz, Germany. LH received lecture/consultant fees from MSD and Actelion, outside the submitted work. TG has received grant support (CARIMA study) and speaker´s honoraria from Novartis, speaker´s honoraria from Boehringer Ingelheim, Daiichi-Sankyo, MSD, Pfizer – Bristol-Myers Squibb, and Astra Zeneca., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Snakebites are considered a significant health issue. Current antivenoms contain polyclonal antibodies, which vary in their specificity against different venom components. Development and characterization of next generation antivenoms including nanobodies against Naja naja oxiana was the main aim of this study. Crude venom was injected into the Sephadex G50 filtration gel chromatography column and then toxic fractions were obtained. Then the corresponding fraction was injected into the HPLC column and the toxic peaks were identified. N. naja oxiana venom was injected into a camel and specific nanobodies screening was performed against the toxic peak using phage display technique. The obtained results showed that among the 12 clones obtained, N24 nanobody was capable of neutralizing P1, the most toxic peak obtained from HPLC chromatography. The molecular weight of P1 was measured with a mass spectrometer and was found to be about seven kDa. The results of the neutralization test of crude N. naja oxiana venom with N24 nanobody showed that 250 μg of recombinant nanobody could neutralize the toxic effects of 20 μg equivalent to LD 50  × 10 of crude venom in mice. The findings indicate the potential of the developed nanobody to serve as a novel antivenom therapy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Introduction: Several studies indicated the ameliorating effects of raloxifene supplementation on apolipoproteins and blood pressure, although others have conflicting findings. Therefore, the present study was conducted in order to accurately and definitively understands the effect of raloxifene on apolipoprotein AI (Apo-AI), apolipoprotein B (APoB), lipoprotein (a) (Lp (a)), systolic blood pressure (SBP) and diastolic blood pressure (DBP) in postmenopausal women., Methods: A systematic literature search was conducted using scientific databases including PubMed, Scopus, Embase, and Web of Science and the Cochrane Library, through May 2024. The quality of studies was assessed using Cochrane tool. Random-effects meta-analysis was used to pool standardized mean differences (SMD) and 95 % CI for the outcomes., Results: Twenty trials, with interventions ranging from 6 to 144 weeks and 2825 participants, were included. Raloxifene supplementation demonstrated significant reductions in ApoB (SMD: -0.92; 95 % CI: -1.49 to -0.35; P = 0.001), and Lp (a) (SMD: -0.25; 95 % CI: -0.39 to -0.11; P < 0.001) while increasing Apo-AI levels (SMD: 0.29; 95 % CI: 0.22-0.36; P < 0.001). Conversely, no significant effects were observed on SBP (WMD: -0.49 mmHg; 95 % CI: -3.01-2.04; P = 0.706), and DBP (WMD: -0.81 mmHg; 95 % CI: -4.04-2.41; P = 0.621). Moreover, subgroup analysis indicated that raloxifene significantly decreased DBP in studies with intervention durations of >12 weeks., Conclusions: This meta-analysis has shown that raloxifene supplementation may have beneficial effects on apolipoproteins in postmenopausal women. Future studies are needed to investigate the effect of raloxifene on health status in in postmenopausal women., Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Objective: Predicting high-value care outcomes is crucial in managing pediatric traumatic brain injuries (TBIs), where timely and accurate prognostication can significantly influence treatment decisions and resource allocation. This study aimed to enhance understanding of how well scoring systems such as the Trauma and Injury Severity Score (TRISS) can forecast high-value care outcomes. Furthermore, the authors compared the predictive power of TRISS with the routinely used Injury Severity Score (ISS)., Methods: The authors performed a retrospective review of their institutional database from June 2016 to June 2023 to identify cases of TBI based on a modified Centers for Disease Control and Prevention framework. Prolonged length of stay (LOS) was defined as a hospital stay falling into the upper quartile of the overall cohort. Discharge to an inpatient rehabilitation facility, acute care hospital, or foster care or death was defined as a nonroutine discharge disposition. Emergency department (ED) transfer to the intensive care unit (ICU) or operating room (OR) was defined as a proxy for severity of injuries. Multivariate logistic regression models were used to explore the association between ISS, TRISS, and high-value care outcomes. The DeLong test was used to assess the differences between the areas under the receiver operating characteristic curve (AUROCs)., Results: This study included 2705 patients with a mean age ± SD of 7.28 ± 5.46 years (63% male). In the overall cohort, 28% experienced prolonged LOS, 7% had a nonroutine discharge disposition from the hospital, and 23% were transferred to the ICU/OR from the ED. In multivariate regression models, both TRISS and ISS were correlated with higher odds of prolonged LOS, nonroutine discharge disposition, and transfer to the ICU/OR from the ED (all p < 0.001). TRISS had a significantly greater AUROC than ISS for nonroutine discharge disposition (0.883 vs 0.849, p < 0.001) and transfer to the ICU/OR (0.898 vs 0.887, p = 0.045), but this result was not significant for prolonged LOS (0.873 vs 0.880, p = 0.140)., Conclusions: TRISS and ISS are effective tools for predicting high-value care outcomes in pediatric TBI. Utilizing these resources can assist healthcare providers in making informed, risk-adjusted predictions.

Objective: Brain metastases (BM) constitute the most common intracranial tumor in adults. Prior literature indicates the 10-year atherosclerotic cardiovascular disease (ASCVD) risk score is associated with increased risk of cancer, potentially attributable to shared risk factors. Understanding the role of ASCVD risk scores in BM may help optimize their care and inform clinical decision-making. Our aim was to explore associations between ASCVD risk score in BM patients and their overall survival, hospital charges, and non-routine discharge disposition., Methods: Electronic medical records were reviewed to collect clinical data for BM patients undergoing surgery at a single institution (2017-2021). Regression analyses were performed accordingly and maximally selected rank statistics were employed to identify an optimal cutoff for ASCVD risk scores. The random survival forest (RSF) machine learning technique identified the most important variable associated with survival outcomes in BM patients., Results: A total of 139 patients were included with average age 62.93±9.29 years, 48.2 % male, 25.2 % with high hospital charges, and 23.7 % experiencing non-routine discharge. Among these patients, 32.3 % had prior history of an ASCVD event, while 67.7 % did not. Overall, this cohort had an average 10-year ASCVD risk score of 12.51±12.98, indicating intermediate risk of ASCVD among all BM patients. On multivariate logistic regression, prior history of ASCVD was associated with higher odds of high hospital charges (OR=3.670, p=0.018), and higher ASCVD risk scores were associated with greater odds of non-routine discharge (OR=1.059, p=0.012). On the multivariate Cox regression model, higher ASCVD risk scores correlated with worse overall survival (HR=1.031, p=0.014). A threshold of 25.1 was identified for high-risk ASCVD scores. Patients with ASCVD scores >25.1 exhibited reduced overall survival in Kaplan-Meier analysis (p=0.015) and multivariate Cox regression (HR: 2.811, p=0.016). Notably, ASCVD risk scores were found to be the most important variable in predicting worse survival outcomes in BM patients compared to other established frailty indices., Conclusion: This study indicates higher ASCVD risk scores in BM patients are associated with worse overall survival. Integrating ASCVD assessment into clinical workflow may facilitate more informed risk-based decision-making., Competing Interests: Declaration of Competing Interest Dr. Chetan Bettegowda is a consultant for Depuy-Synthes, Bionaut Labs, Haystack Oncology and Privo Technologies. Dr. Chetan Bettegowda is a co-founder of Oris-Dx and Belay Diagnostics., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: The role of probiotics and micronutrients in improving immune system function and response to vaccination has been proven. Hence, this study aimed to investigate the effects of probiotics enriched with micronutrients on the immunogenicity of PastoCovac® vaccine., Methods: The probiotic supplement BioBoost® and PastoCovac® vaccine, which contain six expressed receptor-binding domains (RBD) and conjugated with tetanus toxin, were administered concurrently. The safety and efficacy were assessed by determining Immunoglobulin G (IgG) antibody titers to RBD and cytokines, mRNA expression of Toll-like Receptors (TLRs) 5, and clinical symptoms., Results: Results revealed that the administration of the probiotics enriched with micronutrients and vitamins for 14 days before the first vaccine dose, followed by continued supplementation for 14 days after the first dose, and in conjunction with the second vaccine dose, yielded the most significant elevation in interleukin 4 (IL-4), Tumor Necrosis Factor-alpha (TNF alpha), Interferon-gamma (IFN-gamma), and anti-SARS-CoV-2 RBD IgG levels within the supernatant samples collected from spleen cultures with the highest expression of TLR5 genes in intestinal samples, compared to the control group., Conclusion: Our results indicated that the inclusion of probiotics enriched with micronutrients and vitamins significantly enhanced the immunogenicity of the PastoCovac® vaccine. Based on the recommendation to administer third and fourth vaccine doses, particularly for vulnerable and elderly individuals, the utilization of supplements containing probiotics is expected to favorably influence immune responses., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Background: Immunotoxins (ITs) represent a novel class of therapeutics with bifunctional structures that facilitate their penetration through cell membranes to induce target cell destruction. Programmed cell death ligand-1 (PD-L1), a human cell surface protein, is overexpressed in various cancers. This study aimed to construct a novel IT by genetically fusing an anti-PD-L1 Nanobody (Nb) to a truncated diphtheria toxin (DT)., Methods: The IT construct comprised a 127-amino acid anti-PD-L1 Nb fused to a 380-amino acid fragment of DT, with an N-terminal 6x-His tag. Molecular cloning techniques were employed, followed by transformation and verification through colony-PCR, enzyme digestion, and sequencing. The anti-PD-L1 Nb was expressed in WK6 E. coli cells induced by Isopropyl β-D-1- Thiogalactopyranoside (IPTG) and purified from periplasmic extracts using immobilized Metal Ion Affinity hromatography (IMAC). The IT was similarly expressed, purified, and validated via SDS-PAGE and Western blot analysis., Results: ELISA confirmed the binding activity of both Nb and IT to immobilized PD-L1 antigen, whereas truncated DT exhibited no binding. MTT assays demonstrated significant cytotoxicity of IT on A-431 cell lines compared to Nb and truncated DT controls. Statistical analyses underscored the significance of these findings., Conclusion: This study provides a thorough characterization of the constructed IT, highlighting its potential as a therapeutic agent targeting PD-L1-expressing cancer cells. The results support the potential of this IT in cancer immunotherapy, emphasizing the need for further investigation into its efficacy and safety profiles., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Immunotoxins (ITs) are specialized therapeutic agents designed for targeted treatment, particularly in cancer therapy. They consist of a monoclonal antibody or antibody fragment linked to a potent cytotoxic agent, such as bacterial- or plant-derived toxins like diphtheria toxin, ricin, or pseudomonas exotoxin. The monoclonal antibody component specifically binds to antigens expressed on the surface of target cells, facilitating the internalization of the IT. Once inside the cell, the cytotoxic agent is released, disrupting essential cellular processes and leading to cell death. This targeted approach minimizes damage to healthy tissues while effectively eliminating diseased cells. The production of ITs involves two primary methods: recombinant fusion and chemical conjugation. In recombinant fusion, genetic engineering is used to create a fusion protein that combines the antibody and toxin, ensuring precise control over their ratio and functionality. In chemical conjugation, pre-existing antibodies are chemically linked to toxins, allowing for greater flexibility in combining different antibodies and cytotoxic agents. Each method has its advantages and challenges, influencing the specificity, production complexity, and therapeutic potential of the resulting ITs. As research advances, ITs continue to show promise not only in oncology but also in treating other diseases, including inflammatory conditions and atherosclerosis. The precise targeting and potent effects of ITs make them a valuable tool in the development of new therapeutic strategies., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Background: Glioblastoma patients may develop functional deficits post-operatively that affect activities of daily living and result in worse outcomes. The Activity Measure for Post-Acute Care (AM-PAC) instrument assigns patients basic mobility and daily activity scores, but it is unknown if these scores correlate with post-operative outcomes in glioblastoma patients., Methods: Adult (≥18 years) glioblastoma patients evaluated by physical/occupational therapy after resection at a single instution (June 2008-December 2020) were identified. Patient demographics, post-operative AM-PAC scores, and clinical outcomes were collected. Multivariate regression identified associations between AM-PAC scores and post-operative outcomes., Results: 600 patients were included (mean age 59.3 years, 59.2 % male); 151 (25.3 %) and 246 (43.8 %) patients had low mobility (<42.9) and activity (<39.4) scores, respectively. 103 (17.2 %) and 177 (29.5 %) patients experienced extended lengths of stay (LOS) in the ICU (≥2 days) and overall (≥7 days), respectively. 154 (25.7 %) patients had non-home discharges. The 30-day readmission rate was 13.7 %. In multivariate analysis, low mobility scores correlated with increased odds of extended overall (p < 0.0001) and ICU (p = 0.0004) LOS, non-home discharge (p < 0.0001), and 30-day readmission (p = 0.0405). Low activity scores correlated with extended overall LOS (<0.0001) and non-home discharge (p < 0.0001). In log-rank analysis, median survival time was shorter for patients with low mobility (9.5 vs. 14.7 months, p < 0.0001) and activity (10.6 vs. 16.3 months, p < 0.0001) scores than for high-scoring patients., Conclusion: AM-PAC basic mobility and daily activity scores are associated with outcomes after glioblastoma resection. These easily obtainable scores may be useful for prognosticating and guiding decision making in post-operative glioblastoma patients., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Background: The Disintegrin and Metalloproteinase (ADAM) family, also known as the metalloproteinase/disintegrin/cysteine-rich (MDC) proteins, includes both secreted and transmembrane molecules involved in critical biological processes, such as cell migration, adhesion, and signaling. This study aimed to investigate the evolutionary relationships and structural characteristics of disintegrin and metalloproteinase proteins identified in the venom gland transcriptome of the scorpion Hemiscorpius lepturus., Methods: Using bioinformatics tools, we analyzed the open reading frame, conserved motifs, and primary, secondary, and tertiary structures of these proteins. Five proteins, named HLDis- Met1, HLDisMet2, HLDisMet3, HLDisMet4, and HLDisMet5, were identified. Their predicted 3- D structures were within normal ranges (Z-score between -4 to -9)., Results: Phylogenetic analysis revealed that HLDisMet1 shares similarities with proteins from various spider species (Nephila pilipes, Argiope bruennichi, Araneus ventricosus, and Trichonephila inaurata madagascariensis), HLDisMet2 with the scorpion Centruroides sculpturatus, HLDis- Met4 with the scorpion Tityus serrulatus, and HLDisMet5 with several snake species (Python bivittatus, Vipera anatolica senliki, Protobothrops mucrosquamatus, and Naja naja)., Conclusion: These findings highlight the significant similarities between HLDisMet proteins and those found in other venomous species, suggesting a complex and diverse evolutionary pathway for venom components. The cross-species conservation observed may indicate a convergent evolutionary strategy, where different species independently develop similar venom components to adapt to similar ecological niches or prey types. This study highlights the evolutionary significance of venom diversification and its potential applications in understanding venom biology across different species., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

The use of mRNA and ribonucleoproteins (RNPs) as therapeutic agents is a promising strategy for treating diseases such as cancer and infectious diseases. This review provides recent advancements and challenges in mRNA- and RNP-based therapies, focusing on delivery systems such as lipid nanoparticles (LNPs), which ensure efficient delivery to target cells. Strategies such as microfluidic devices are employed to prepare LNPs loaded with mRNA and RNPs, demonstrating effective genome editing and protein expression in vitro and in vivo . These applications extend to cancer treatment and infectious disease management, with promising results in genome editing for cancer therapy using LNPs encapsulating Cas9 mRNA and single-guide RNA. In addition, tissue-specific targeting strategies offer potential for improved therapeutic outcomes and reduced off-target effects. Despite progress, challenges such as optimizing delivery efficiency and targeting remain. Future research should enhance delivery efficiency, explore tissue-specific targeting, investigate combination therapies, and advance clinical translation. In conclusion, mRNA- and RNP-based therapies offer a promising avenue for treating various diseases and have the potential to revolutionize medicine, providing new hope for patients worldwide., Competing Interests: The authors declare no competing interests., (© 2024 The Author(s).)

Leukemia, a type of blood cancer marked by an abnormal increase in white blood cells, poses a significant challenge to healthcare. The key to successful treatment lies in early detection. However, traditional methods often fall short. This review investigates the potential of electrochemical biosensors for a more accurate and earlier diagnosis of leukemia. Electrochemical biosensors are compact devices that transform biological interactions into electrical signals. Their small size, ease of use, and minimal sample requirements make them perfectly suited for point-of-care applications. Their remarkable sensitivity and specificity enable the detection of subtle biomolecular changes associated with leukemia, which is crucial for early disease detection. This review delves into studies that have utilized these biosensors to identify various types of leukemia. It examines the roles of electrodes, biorecognition elements, and signal transduction mechanisms. The discussion includes the integration of nanomaterials such as gold nanoparticles and nitrogen-doped graphene into biosensor design. These materials boost sensitivity, enhance signal amplification, and facilitate multi-analyte detection, thereby providing a more holistic view of the disease. Beyond technical advancements, the review underscores the practical benefits of these biosensors. Their portability makes them a promising tool for resource-constrained settings, enabling swift diagnosis in remote areas or at a patient's bedside. The potential for monitoring treatment effectiveness and detecting minimal residual disease to prevent relapse is also explored. This review emphasizes the transformative potential of electrochemical biosensors in combating leukemia. By facilitating earlier and more accurate diagnosis, these biosensors stand to revolutionize patient care and enhance treatment outcomes., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Burnout is recognized as a negative contributor to well-being within healthcare. The purpose of this study was to evaluate whether participation in the Peace Education Program (PEP), a 10-week wellness course, promoted durable improvement of burnout and wellness in a large academic medical center. We deployed PEP virtually through a series of free, interactive, online wellness sessions focusing on 10 domains: peace, appreciation, inner strength, self-awareness, clarity, understanding, dignity, choice, hope, and contentment. Two weeks before and six months following the sessions, we distributed de-identified surveys based upon the validated Schwartz Outcome Scale-10 (SOS-10) with Likert scale scores 0-6 to assess participant wellness. The Mann-Whitney U test was used to analyze statistical differences between post- and pre-course data. Sixty-nine faculty, trainees, and staff registered to participate, 21 participants completed the pre-course survey and 10 completed the post-course survey. The pre-course mean + standard error (SE) SOS-10 score was 43.2 ± 0.8, which improved to 51.0 ± 0.7 post-course ( P < 0.001). Overall, all 10 domains demonstrated improved mean scores over time, with the five domains of "inner strength" ( P = 0.008), "understanding" ( P = 0.030), "peace" ( P = 0.048), "choice" ( P = 0.026), and "hope" ( P = 0.020) demonstrating statistically significant score improvements six months after course completion. Participants completing ≥7 sessions benefited more than their counterparts and achieved statistically significant improvement in SOS-10 wellness scores (8.77 points) six months after course completion. Our findings demonstrate improved wellness in participants following peace education course completion. This free, interactive, online course may be utilized at other medical centers to improve wellness., Competing Interests: There are no conflicts of interest., (Copyright: © 2024 Journal of Education and Health Promotion.)

Background and Objectives: Surgery for the very elderly is a progressively important paradigm as life expectancy continues to rise. Patients with glioblastoma multiforme often undergo surgery, radiotherapy (RT), and chemotherapy (CT) to prolong overall survival (OS). However, the efficacy of these treatment modalities in patients aged 80 years and older has yet to be fully assessed in the literature., Methods: The National Cancer Database was used to retrospectively identify patients aged 65 years and older with glioblastoma multiforme (1989-2016). All available patient demographic characteristics, disease characteristics, and clinical outcomes were collected. To study OS, bivariable survival models were created using Kaplan-Meier estimates. A Cox proportional-hazards model was used for final adjusted analyses., Results: A total of 578 very elderly patients (aged 80 years and older) and 2836 elderly patients (aged 65-79 years) were identified. Compared with elderly patients, very elderly patients were more likely to have Medicare (odds ratio [OR] 1.899 [95% CI: 1.417-2.544], P < .001) while less likely to have private insurance status (OR 0.544 [95% CI: 0.401-0.739], P < .001). In addition, very elderly patients were more likely to travel the least distance for treatment and have multiple tumors (P < .001). When controlling for demographic and disease characteristics, very elderly patients were less likely to receive gross total resection (GTR) (OR 0.822 [95% CI: 0.681-0.991], P < .041), RT (OR 0.385 [95% CI: 0.319-0.466], P < .001), or postoperative CT (OR 0.298 [95% CI: 0.219-0.359], P < .001) relative to elderly counterparts. Within very elderly patients, GTR, RT, and CT all independently and significantly predicted improved OS (P < .001 for all). These predictive models were deployed in an online calculator (https://spine.shinyapps.io/GBM&#95;elderly)., Conclusion: Very elderly patients are less likely to receive GTR, RT, or CT when compared with elderly counterparts despite use of these therapies conferring improved OS. Selected very elderly patients may benefit from more aggressive attempts at surgical and adjuvant treatment., (Copyright © Congress of Neurological Surgeons 2024. All rights reserved.)

Background and Aims: The primary objective of this systematic review and meta-analysis was to assess the impact of dextrose prolotherapy on individuals diagnosed with knee osteoarthritis (KOA)., Methods: To conduct a thorough investigation, a variety of leading international databases were checked, including PubMed (Medline), Scopus, Web of Sciences, EMBASE (Elsevier), ClinicalTrials.gov, and the Cochrane Library. The search covered a period from January 2000 to the end of June 2023, which facilitated the collection of relevant studies., Results: The findings of the study revealed that when the studies utilizing the Western Ontario McMaster Universities Index tool (WOMAC) were combined, patients with KOA who received prolotherapy experienced an improvement in function compared with those who received other treatments (SMD: 0.20; 95% Confidence Interval [1]: -0.11, 0.51; p value SMD = 0.221; I 2 : 78.49%; p heterogeneity  < 0.001). Additionally, there was a decrease in mean pain and stiffness among patients who received prolotherapy compared with those who received other treatments or a placebo [(SMD: -0.95; 95% CI: -1.14, -0.76; p value SMD < 0.001; I 2 : 59.35%; p heterogeneity  = 0.070) and (SMD: -0.21; 95% CI: -0.32, -0.10; p value SMD < 0.001; I 2 : 88.11%; p heterogeneity  < 0.001)]. Furthermore, based on the Visual Analog Scale (VAS) score, there was a reduction of 0.81 units out of 10 in mean pain for patients with KOA who received prolotherapy (SMD: -0.81; 95% CI: -5.63, 4.10; p value SMD = 0.693; I 2 : 48.54%; p heterogeneity  = 0.08)., Conclusion: Drawing from the data analysis performed in this meta-analysis, it is apparent that dextrose prolotherapy exhibits promising effectiveness in reducing joint pain and stiffness, as well as improving functional performance in individuals suffering from KOA. Furthermore, it is recommended that forthcoming studies incorporate follow-up periods to guide decisions concerning the duration of prolotherapy's effects., Competing Interests: The authors declare no conflict of interest., (© 2024 The Author(s). Health Science Reports published by Wiley Periodicals LLC.)

Advanced molecular testing has increasingly become an integral component for accurate diagnosis of central nervous system (CNS) tumors. We sought to establish the current state of molecular testing availability and approaches for the diagnosis of CNS tumors in US hospitals that conduct high volumes of CNS tumor resections. We distributed a 16-item survey inquiring about molecular testing approaches for CNS tumors to 115 neuropathologists at US hospitals with neurosurgery residency programs. Thirty-five neuropathologists (30.4%) responded to the survey, all of whom indicated their institutions perform molecular testing on CNS tumor tissue. The most commonly offered tests were MGMT methylation profiling and next-generation sequencing. Fourteen respondents (40%) indicated that their institution is able to test for and report all of the molecular alterations included in our survey. Nine (25.7%) respondents indicated that molecular testing is performed as standard of care for all patients with resected CNS tumors. Our results suggest that even in academic hospitals with a high volume of CNS tumor resections, molecular testing for these tumors is limited. Continued initiatives are necessary to expand the availability of molecular testing for CNS tumors to ensure diagnostic accuracy and guide targeted therapy., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Association of Neuropathologists, Inc.)

Objective: Spinal chordoma treatment guidelines recommend resection. However, in patients in whom gross-total resection (GTR) is achieved, the benefits of radiation therapy (RT) are unclear. Therefore, the authors performed a systematic review to determine if RT is associated with postoperative progression-free survival (PFS) or overall survival (OS) after achieving GTR of spinal chordoma., Methods: The PubMed database was searched for studies including individualized data of patients undergoing GTR with or without RT for spinal chordoma. Patients < 18 years of age or those who underwent stereotactic body RT were excluded. Qualitative assessment was performed using Newcastle-Ottawa Scale guidelines. Log-rank tests for time-to-event data and a Cox proportional-hazards model were generated for a multivariable statistical model., Results: Complete data of 132 patients were retrieved, with 37 (28%) patients receiving adjuvant RT and 95 (72%) not receiving adjuvant RT. The mean follow-up was not statistically significantly different between those undergoing RT and not undergoing RT (54.02 months and 65.43 months, respectively). Patients were more likely not to undergo RT if their disease was located in the sacrum versus the mobile spine (p < 0.001). When controlling for age ≥ 65 years, male sex, disease location, and treatment year ≥ 2010, patients undergoing RT had similar PFS and OS when compared with those not undergoing RT on multivariable survival analysis (HR 0.935 [95% CI 0.703-2.340], p = 0.844 and HR 2.078 [95% CI 0.848-5.090], p = 0.110, respectively). However, age ≥ 65 years was associated with poorer OS in adjusted analyses (HR 2.761 [95% CI 1.185-6.432], p = 0.018) relative to patients < 65 years of age., Conclusions: After achieving GTR of spinal chordoma, the utility of RT on PFS and OS remains unclear. Age ≥ 65 years appears to be associated with OS in spinal chordoma patients. Additional multicenter prospective studies are needed to determine the utility of RT in this patient population.

Organophosphorylated coumarin derivatives were synthesized by a three-component reaction of 4-chloro-3-formylcoumarin, aromatic amines, and dialkyl phosphite in the presence of ZnCl 2 . This process includes the formation of C(sp 3 )-P and C(sp 2 )-N bonds in one pot. The modular scope of the reaction allowed rapid access to a variety of 6 H -chromeno[4,3- b ]quinolin-7-ylphosphonate derivatives in good yields. Furthermore, photophysical studies of the products revealed their stimulating fluorescence properties.