10 results on '"Escuin S"'
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2. Fire severity assessment by using NBR (Normalized Burn Ratio) and NDVI (Normalized Difference Vegetation Index) derived from LANDSAT TM/ETM images.
- Author
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Escuin, S., Navarro, R., and Fernández, P.
- Subjects
- *
LANDSAT satellites , *SCIENTIFIC satellites , *ARTIFICIAL satellites , *PROBABILITY measures , *DIGITAL images , *WILDFIRES , *NEAR infrared spectroscopy , *IMAGE processing - Abstract
In this work, the capacity of NBR and NDVI indices derived from LANDSAT TM/ETM images has been analysed for fire severity assessment. For this purpose, three fires occurring in southern Spain were studied. Firstly, the displacements of burned and unburned pixels in the pre-/post-fire NIR-MIR and NIR-R bi-spectral spaces were analysed with the aim of establishing which of the two indices was the most sensitive for discriminating severity levels. Then, the capacity of the two indices, both from a uni-temporal (post-fire) and bi-temporal perspective (pre and post-fire), to discriminate three severity levels was studied. Based on the results, it was decided that the most suitable way to assess wildfire severity by index segmentation was to discriminate between unburned and burned pixels according to their NBR pre-/post-fire difference values (dNBR), and, subsequently, to distinguish between pixels with an extreme and moderate severity based on the NBR post-fire values. The thresholds calculated for these indices permitted fire severity mapping with an accuracy of 86.42% (±4.31%). These thresholds could be extrapolated to other fires with similar characteristics although a calculation of their own specific thresholds could improve the accuracy of the fire severity map obtained. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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3. Dual mechanism underlying failure of neural tube closure in the Zic2 mutant mouse.
- Author
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Escuin S, Rose Raza-Knight S, Savery D, Gaston-Massuet C, Galea GL, Greene NDE, and Copp AJ
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- Mice, Animals, Humans, Neural Tube metabolism, Actomyosin metabolism, Neurulation, Mammals metabolism, Nuclear Proteins metabolism, Transcription Factors metabolism, Spinal Dysraphism, Neural Tube Defects genetics
- Abstract
Understanding the molecular mechanisms that lead to birth defects is an important step towards improved primary prevention. Mouse embryos homozygous for the Kumba (Ku) mutant allele of Zic2 develop severe spina bifida with complete lack of dorsolateral hinge points (DLHPs) in the neuroepithelium. Bone morphogenetic protein (BMP) signalling is overactivated in Zic2Ku/Ku embryos, and the BMP inhibitor dorsomorphin partially rescues neural tube closure in cultured embryos. RhoA signalling is also overactivated, with accumulation of actomyosin in the Zic2Ku/Ku neuroepithelium, and the myosin inhibitor Blebbistatin partially normalises neural tube closure. However, dorsomorphin and Blebbistatin differ in their effects at tissue and cellular levels: DLHP formation is rescued by dorsomorphin but not Blebbistatin, whereas abnormal accumulation of actomyosin is rescued by Blebbistatin but not dorsomorphin. These findings suggest a dual mechanism of spina bifida origin in Zic2Ku/Ku embryos: faulty BMP-dependent formation of DLHPs and RhoA-dependent F-actin accumulation in the neuroepithelium. Hence, we identify a multi-pathway origin of spina bifida in a mammalian system that may provide a developmental basis for understanding the corresponding multifactorial human defects., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2023. Published by The Company of Biologists Ltd.)
- Published
- 2023
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4. Rho GTPases in mammalian spinal neural tube closure.
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Rolo A, Escuin S, Greene NDE, and Copp AJ
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- Animals, Cell Membrane metabolism, Humans, Neural Tube cytology, Neural Tube enzymology, Neural Tube metabolism, Spinal Cord embryology, rho GTP-Binding Proteins metabolism
- Abstract
Neural tube closure is an important morphogenetic event that involves dramatic reshaping of both neural and non-neural tissues. Rho GTPases are key cytoskeletal regulators involved in cell motility and in several developmental processes, and are thus expected to play pivotal roles in neurulation. Here, we discuss 2 recent studies that shed light on the roles of distinct Rho GTPases in different tissues during neurulation. RhoA plays an essential role in regulating actomyosin dynamics in the neural epithelium of the elevating neural folds, while Rac1 is required for the formation of cell protrusions in the non-neural surface ectoderm during neural fold fusion.
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- 2018
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5. Microtubules, polarity and vertebrate neural tube morphogenesis.
- Author
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Cearns MD, Escuin S, Alexandre P, Greene ND, and Copp AJ
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- Animals, Cell Polarity, Cell Shape, Humans, Neuroepithelial Cells physiology, Microtubules physiology, Neural Tube embryology, Neurulation
- Abstract
Microtubules (MTs) are key cellular components, long known to participate in morphogenetic events that shape the developing embryo. However, the links between the cellular functions of MTs, their effects on cell shape and polarity, and their role in large-scale morphogenesis remain poorly understood. Here, these relationships were examined with respect to two strategies for generating the vertebrate neural tube: bending and closure of the mammalian neural plate; and cavitation of the teleost neural rod. The latter process has been compared with 'secondary' neurulation that generates the caudal spinal cord in mammals. MTs align along the apico-basal axis of the mammalian neuroepithelium early in neural tube closure, participating functionally in interkinetic nuclear migration, which indirectly impacts on cell shape. Whether MTs play other functional roles in mammalian neurulation remains unclear. In the zebrafish, MTs are important for defining the neural rod midline prior to its cavitation, both by localizing apical proteins at the tissue midline and by orienting cell division through a mirror-symmetric MT apparatus that helps to further define the medial localization of apical polarity proteins. Par proteins have been implicated in centrosome positioning in neuroepithelia as well as in the control of polarized morphogenetic movements in the neural rod. Understanding of MT functions during early nervous system development has so far been limited, partly by techniques that fail to distinguish 'cause' from 'effect'. Future developments will likely rely on novel ways to selectively impair MT function in order to investigate the roles they play., (© 2016 Anatomical Society.)
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- 2016
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6. Regulation of cell protrusions by small GTPases during fusion of the neural folds.
- Author
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Rolo A, Savery D, Escuin S, de Castro SC, Armer HE, Munro PM, Molè MA, Greene ND, and Copp AJ
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- Animals, Mice, Neurulation, Cell Surface Extensions metabolism, Ectoderm cytology, Ectoderm enzymology, Neural Crest embryology, Neural Tube embryology, Neuropeptides metabolism, cdc42 GTP-Binding Protein metabolism, rac1 GTP-Binding Protein metabolism
- Abstract
Epithelial fusion is a crucial process in embryonic development, and its failure underlies several clinically important birth defects. For example, failure of neural fold fusion during neurulation leads to open neural tube defects including spina bifida. Using mouse embryos, we show that cell protrusions emanating from the apposed neural fold tips, at the interface between the neuroepithelium and the surface ectoderm, are required for completion of neural tube closure. By genetically ablating the cytoskeletal regulators Rac1 or Cdc42 in the dorsal neuroepithelium, or in the surface ectoderm, we show that these protrusions originate from surface ectodermal cells and that Rac1 is necessary for the formation of membrane ruffles which typify late closure stages, whereas Cdc42 is required for the predominance of filopodia in early neurulation. This study provides evidence for the essential role and molecular regulation of membrane protrusions prior to fusion of a key organ primordium in mammalian development.
- Published
- 2016
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7. Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion.
- Author
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Quétier I, Marshall JJT, Spencer-Dene B, Lachmann S, Casamassima A, Franco C, Escuin S, Worrall JT, Baskaran P, Rajeeve V, Howell M, Copp AJ, Stamp G, Rosewell I, Cutillas P, Gerhardt H, Parker PJ, and Cameron AJM
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- Animals, Antineoplastic Agents pharmacology, Apoptosis drug effects, Cell Line, Cell Movement drug effects, Cell Proliferation drug effects, Embryo, Mammalian cytology, Embryo, Mammalian metabolism, Embryonic Development drug effects, Genes, Reporter, Heart growth & development, Mesoderm cytology, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Electron, Scanning, Myocardium metabolism, Myocardium pathology, Protein Kinase C deficiency, Protein Kinase C metabolism, Tamoxifen analogs & derivatives, Tamoxifen pharmacology, Mesoderm metabolism, Protein Kinase C genetics
- Abstract
In animals, the protein kinase C (PKC) family has expanded into diversely regulated subgroups, including the Rho family-responsive PKN kinases. Here, we describe knockouts of all three mouse PKN isoforms and reveal that PKN2 loss results in lethality at embryonic day 10 (E10), with associated cardiovascular and morphogenetic defects. The cardiovascular phenotype was not recapitulated by conditional deletion of PKN2 in endothelial cells or the developing heart. In contrast, inducible systemic deletion of PKN2 after E7 provoked collapse of the embryonic mesoderm. Furthermore, mouse embryonic fibroblasts, which arise from the embryonic mesoderm, depend on PKN2 for proliferation and motility. These cellular defects are reflected in vivo as dependence on PKN2 for mesoderm proliferation and neural crest migration. We conclude that failure of the mesoderm to expand in the absence of PKN2 compromises cardiovascular integrity and development, resulting in lethality., (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2016
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8. Rho-kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closure.
- Author
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Escuin S, Vernay B, Savery D, Gurniak CB, Witke W, Greene ND, and Copp AJ
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- Actins genetics, Actomyosin genetics, Animals, Cofilin 1 genetics, Cofilin 1 metabolism, Lim Kinases genetics, Lim Kinases metabolism, Mice, Mice, Mutant Strains, rho GTP-Binding Proteins genetics, rho GTP-Binding Proteins metabolism, rho-Associated Kinases genetics, rhoA GTP-Binding Protein, Actins metabolism, Actomyosin metabolism, Neural Tube embryology, rho-Associated Kinases metabolism
- Abstract
The cytoskeleton is widely considered essential for neurulation, yet the mouse spinal neural tube can close despite genetic and non-genetic disruption of the cytoskeleton. To investigate this apparent contradiction, we applied cytoskeletal inhibitors to mouse embryos in culture. Preventing actomyosin cross-linking, F-actin assembly or myosin II contractile activity did not disrupt spinal closure. In contrast, inhibiting Rho kinase (ROCK, for which there are two isoforms ROCK1 and ROCK2) or blocking F-actin disassembly prevented closure, with apical F-actin accumulation and adherens junction disturbance in the neuroepithelium. Cofilin-1-null embryos yielded a similar phenotype, supporting the hypothesis that there is a key role for actin turnover. Co-exposure to Blebbistatin rescued the neurulation defects caused by RhoA inhibition, whereas an inhibitor of myosin light chain kinase, ML-7, had no such effect. We conclude that regulation of RhoA, Rho kinase, LIM kinase and cofilin signalling is necessary for spinal neural tube closure through precise control of neuroepithelial actin turnover and actomyosin disassembly. In contrast, actomyosin assembly and myosin ATPase activity are not limiting for closure., (© 2015. Published by The Company of Biologists Ltd.)
- Published
- 2015
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9. Mutations in the planar cell polarity genes CELSR1 and SCRIB are associated with the severe neural tube defect craniorachischisis.
- Author
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Robinson A, Escuin S, Doudney K, Vekemans M, Stevenson RE, Greene ND, Copp AJ, and Stanier P
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- Animals, Cadherins metabolism, Cell Line, Dogs, Fetus, Gene Order, Humans, Immunoprecipitation, Membrane Proteins metabolism, Mutation, Missense, Neural Tube Defects metabolism, Protein Transport genetics, Tumor Suppressor Proteins metabolism, Cadherins genetics, Genetic Predisposition to Disease, Membrane Proteins genetics, Mutation, Neural Tube Defects genetics, Tumor Suppressor Proteins genetics
- Abstract
Craniorachischisis (CRN) is a severe neural tube defect (NTD) resulting from failure to initiate closure, leaving the hindbrain and spinal neural tube entirely open. Clues to the genetic basis of this condition come from several mouse models, which harbor mutations in core members of the planar cell polarity (PCP) signaling pathway. Previous studies of humans with CRN failed to identify mutations in the core PCP genes, VANGL1 and VANGL2. Here, we analyzed other key PCP genes: CELSR1, PRICKLE1, PTK7, and SCRIB, with the finding of eight potentially causative mutations in both CELSR1 and SCRIB. Functional effects of these unique or rare human variants were evaluated using known protein-protein interactions as well as subcellular protein localization. While protein interactions were not affected, variants from five of the 36 patients exhibited a profound alteration in subcellular protein localization, with diminution or abolition of trafficking to the plasma membrane. Comparable effects were seen in the crash and spin cycle mouse Celsr1 mutants, and the line-90 mouse Scrib mutant. We conclude that missense variants in CELSR1 and SCRIB may represent a cause of CRN in humans, as in mice, with defective PCP protein trafficking to the plasma membrane a likely pathogenic mechanism., (© 2011 Wiley Periodicals, Inc.)
- Published
- 2012
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10. Integrin alpha5beta1 is necessary for regulation of radial migration of cortical neurons during mouse brain development.
- Author
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Marchetti G, Escuin S, van der Flier A, De Arcangelis A, Hynes RO, and Georges-Labouesse E
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- Animals, Cell Differentiation physiology, Cells, Cultured, Electroporation methods, HeLa Cells, Humans, Integrin alpha5beta1 genetics, Mice, Mice, Knockout, Neurons cytology, RNA Interference, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Cell Movement physiology, Cerebral Cortex cytology, Cerebral Cortex embryology, Cerebral Cortex growth & development, Integrin alpha5beta1 metabolism, Neurons physiology
- Abstract
During cerebral cortex development, post-mitotic neurons interact with radial glial fibers and the extracellular environment to migrate away from the ventricular region and form a correct laminar structure. Integrin receptors are major mediators of cell-cell and cell-extracellular matrix interactions. Several integrin heterodimers are present during formation of the cortical layers. The alpha5beta1 receptor is expressed in the neural progenitors of the ventricular zone during cerebral cortex formation. Using in utero electroporation to introduce short hairpin RNAs in the brain at embryonic day 15.5, we were able to inhibit acutely the expression of alpha5 integrin in the developing cortex. The knockdown of alpha5 integrin expression level in neural precursors resulted in an inhibition of radial migration, without perturbing the glial scaffold. Moreover, the same inhibitory effect on neuronal migration was observed after electroporation of a Cre recombinase expression plasmid into the neural progenitors of conditional knockout mice for alpha5 integrin. In both types of experiments, the electroporated cells expressing reduced levels of alpha5 integrin accumulated in the premigratory region with an abnormal morphology. At postnatal day 2, ectopic neurons were observed in cortical layer V, while a deficit of neurons was observed in cortical layer II-IV. We show that these neurons do not express a layer V-specific marker, suggesting that they have not undergone premature differentiation. Overall, these results indicate that alpha5beta1 integrin functions in the regulation of neural morphology and migration during cortical development, playing a role in cortical lamination.
- Published
- 2010
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